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https://www.readbyqxmd.com/read/27891064/proteomic-based-approach-for-characterizing-4-hydroxy-2-nonenal-induced-oxidation-of-buffalo-bubalus-bubalis-and-goat-capra-hircus-meat-myoglobins
#1
Naveena B Maheswarappa, K Usha Rani, Y Praveen Kumar, Vinayak V Kulkarni, Srikanth Rapole
BACKGROUND: Myoglobin (Mb) is a sarcoplasmic heme protein primarily responsible for meat color and its chemistry is species specific. 4-hydroxy-2-nonenal (HNE) is a cytotoxic lipid derived aldehyde detected in meat and was reported to covalently adduct with nucleophilic histidine residues of Mb and predispose it to greater oxidation. However, no literature is available on characterization of lipid oxidation induced oxidation of Indian water buffalo (Bubalus bubalis) and goat (Capra hircus) myoglobins...
2016: Proteome Science
https://www.readbyqxmd.com/read/27870918/time-dependent-impact-of-irreversible-electroporation-on-pancreas-liver-blood-vessels-and-nerves-a-systematic-review-of-experimental-studies
#2
J A Vogel, E van Veldhuisen, P Agnass, J Crezee, F Dijk, J Verheij, T M van Gulik, M R Meijerink, L G Vroomen, K P van Lienden, M G Besselink
INTRODUCTION: Irreversible electroporation (IRE) is a novel ablation technique in the treatment of unresectable cancer. The non-thermal mechanism is thought to cause mostly apoptosis compared to necrosis in thermal techniques. Both in experimental and clinical studies, a waiting time between ablation and tissue or imaging analysis to allow for cell death through apoptosis, is often reported. However, the dynamics of the IRE effect over time remain unknown. Therefore, this study aims to summarize these effects in relation to the time between treatment and evaluation...
2016: PloS One
https://www.readbyqxmd.com/read/27798673/identification-and-expression-profiling-of-mirnaome-in-goat-longissimus-dorsi-muscle-from-prenatal-stages-to-a-neonatal-stage
#3
Jiazhong Guo, Wei Zhao, Siyuan Zhan, Li Li, Tao Zhong, Linjie Wang, Yao Dong, Hongping Zhang
Skeletal muscle development is a complex biological process regulated by numerous genes and non-coding RNAs, such as microRNAs (miRNAs). In the current study, we made use of the deep sequencing data from Jianzhou Da'er goat longissimus dorsi sampled on days 45, 60, and 105 of gestation, as well as day three after birth to identify miRNAs that regulate goat skeletal myogenesis, and examine their temporal expression profiles. A total of 410 known goat miRNAs, 752 miRNA homologs and 88 novel miRNAs were identified across four stages...
2016: PloS One
https://www.readbyqxmd.com/read/27744371/distribution-and-depletion-of-ractopamine-in-goat-plasma-urine-and-various-muscle-tissues
#4
Zhen Zhao, Xu Gu, Xiaoou Su, Junguo Li, Jun Li, Yingchao Dong, Yujuan Yang, Ting Yao, Yuchang Qin
This study investigated the ractopamine (RAC) distribution and depletion process in goat plasma, urine and various muscle tissues which were associated with a potential risk for consumer health. The experiment was carried out in 21 goats (18 treated and 3 controls). Treated animals were administered orally a dose of 1 mg/kg body mass per day for 28 consecutive days and randomly sacrificed on Days 0.25, 1, 3, 7, 14 and 21 of the withdrawal period. RAC in goat samples was analyzed by using ultra-high performance liquid chromatography-quadrupole-orbitrap high-resolution mass spectrometry...
October 15, 2016: Journal of Analytical Toxicology
https://www.readbyqxmd.com/read/27743852/the-mixture-of-liquid-foam-soap-ethanol-and-citric-acid-as-a-new-fixative-preservative-solution-in-veterinary-anatomy
#5
Erkut Turan, Ozay Gules, Figen Sevil Kilimci, Mehmet Erkut Kara, Omer Gurkan Dilek, Seyyid Said Sabanci, Musa Tatar
The present study investigates the efficiency of liquid foam soap, ethanol, citric acid and benzalkonium chloride as a fixative-preservative solution (a soap-and ethanol-based fixing solution, or SEFS). In this study, ethanol serves as the fixative and preservative, liquid foam soap as the modifying agent, citric acid as the antioxidant and benzalkonium chloride as the disinfectant. The goat cadavers perfused with SEFS (n=8) were evaluated over a period of one year with respect to hardness, colour and odour using objective methods...
October 13, 2016: Annals of Anatomy, Anatomischer Anzeiger: Official Organ of the Anatomische Gesellschaft
https://www.readbyqxmd.com/read/27741564/the-anatomy-of-caprine-female-urethra-and-characteristics-of-muscle-and-bone-marrow-derived-caprine-cells-for-autologous-cell-therapy-testing
#6
A Burdzinska, B Dybowski, W Zarychta-Wisniewska, A Kulesza, R Zagozdzon, Z Gajewski, L Paczek
Cell therapy is emerging as an alternative treatment of stress urinary incontinence. However, many aspects of the procedure require further optimization. A large animal model is needed to reliably test cell delivery methods. In this study we aim to determine suitability of the goat as an experimental animal for testing intraurethral autologous cell transplantation in terms of urethral anatomy and cell culture parameters. The experiments were performed in 12 mature/aged female goats. Isolated caprine muscle derived cells (MDC) were myogenic in vitro and mesenchymal stem cells (MSC) population was able to differentiate into adipo-, osteo- and chondrogenic lineages...
October 14, 2016: Anatomical Record: Advances in Integrative Anatomy and Evolutionary Biology
https://www.readbyqxmd.com/read/27736224/benign-peripheral-nerve-sheath-tumor-in-a-wild-toco-toucan-ramphastos-toco
#7
Marcelo P N Carvalho, Natalia C C A Fernandes, Viviane C Nemer, Ramiro N Dias Neto, Rodrigo H F Teixeira, Bruna S Miranda, Maria J Mamprim, José L Catão-Dias, Rodrigo A Réssio
Peripheral nerve sheath tumors are a heterogeneous group of neoplasms that comprise neurofibromas, schwannomas, neurilemmomas, and perineuromas. In animals, peripheral nerve sheath neoplasms are most commonly diagnosed in dogs and cattle, followed by horses, goats, and cats, but their occurrence is uncommon in birds. An adult, free-living, male toco (common) toucan ( Ramphastos toco ) was admitted to the zoo animal clinic with weight loss, dehydration, and presence of a soft nodule adhered to the medial portion of the left pectoral muscle...
September 2016: Journal of Avian Medicine and Surgery
https://www.readbyqxmd.com/read/27605760/a-survey-on-sarcocystis-spp-infection-in-cattle-of-tabriz-city-iran
#8
Mohammad Mirzaei, Hadi Rezaei
Sarcocystis is one of the most prevalent protozoan parasites in the striated muscles of livestock slaughtered for food such as cattle, sheep and goat. Meat that is heavily infected may be condemned as unfit for human consumption. The aim of this study was to determine the prevalence of Sarcocystis spp. in slaughtered cattle in Tabriz, northwest of Iran. The prevalence of Sarcocystis spp. infection was investigated in 670 cattle, slaughtered from April 2013 to October 2013 in the Tabriz abattoir, Iran using naked eye examination for macroscopic Sarcocysts, and peptic digestion, muscle squash, squeezing methods for microscopic types...
September 2016: Journal of Parasitic Diseases: Official Organ of the Indian Society for Parasitology
https://www.readbyqxmd.com/read/27561543/inferring-the-skeletal-muscle-developmental-changes-of-grazing-and-barn-fed-goats-from-gene-expression-data
#9
Jinyu Huang, Jinzhen Jiao, Zhi-Liang Tan, Zhixiong He, Karen A Beauchemin, Robert Forster, Xue-Feng Han, Shao-Xun Tang, Jinghe Kang, Chuanshe Zhou
Thirty-six Xiangdong black goats were used to investigate age-related mRNA and protein expression levels of some genes related to skeletal muscle structural proteins, MRFs and MEF2 family, and skeletal muscle fiber type and composition during skeletal muscle growth under grazing (G) and barn-fed (BF) feeding systems. Goats were slaughtered at six time points selected to reflect developmental changes of skeletal muscle during nonrumination (days 0, 7, and 14), transition (day 42), and rumination phases (days 56 and 70)...
September 14, 2016: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/27554596/increase-in-antioxidant-activity-by-sheep-goat-whey-protein-through-nuclear-factor-like-2-nrf2-is-cell-type-dependent
#10
Efthalia Kerasioti, Dimitrios Stagos, Aggeliki Tzimi, Dimitrios Kouretas
The aim of the present study was to investigate the molecular mechanisms through which sheep/goat whey protein exerts its antioxidant activity. Thus, it was examined whey protein's effects on the expression of transcription factor, nuclear factor-like 2 (Nrf2) and on the expression and activity of a number of antioxidant and phase II enzymes, superoxide dismutase (SOD), catalase (CAT), heme oxygenase 1 (HO-1), synthase glutamyl cysteine (GCS) and glutathione-s-transferase (GST), in muscle C2C12 and EA.hy926 endothelial cells...
August 20, 2016: Food and Chemical Toxicology
https://www.readbyqxmd.com/read/27550073/genome-wide-identification-and-characterization-of-long-non-coding-rnas-in-developmental-skeletal-muscle-of-fetal-goat
#11
Siyuan Zhan, Yao Dong, Wei Zhao, Jiazhong Guo, Tao Zhong, Linjie Wang, Li Li, Hongping Zhang
BACKGROUND: Long non-coding RNAs (lncRNAs) have been studied extensively over the past few years. Large numbers of lncRNAs have been identified in mouse, rat, and human, and some of them have been shown to play important roles in muscle development and myogenesis. However, there are few reports on the characterization of lncRNAs covering all the development stages of skeletal muscle in livestock. RESULTS: RNA libraries constructed from developing longissimus dorsi muscle of fetal (45, 60, and 105 days of gestation) and postnatal (3 days after birth) goat (Capra hircus) were sequenced...
2016: BMC Genomics
https://www.readbyqxmd.com/read/27461387/efficient-talen-mediated-myostatin-gene-editing-in-goats
#12
Baoli Yu, Rui Lu, Yuguo Yuan, Ting Zhang, Shaozheng Song, Zhengqiang Qi, Bin Shao, Mengmin Zhu, Fei Mi, Yong Cheng
BACKGROUND: Myostatin (MSTN) encodes a negative regulator of skeletal muscle mass that might have applications for promoting muscle growth in livestock. In this study, we aimed to test whether targeted MSTN editing, mediated by transcription activator-like effector nucleases (TALENs), is a viable approach to create myostatin-modified goats (Capra hircus). RESULTS: We obtained a pair of TALENs (MTAL-2) that could recognize and cut the targeted MSTN site in the goat genome...
2016: BMC Developmental Biology
https://www.readbyqxmd.com/read/27417210/generation-and-evaluation-of-myostatin-knock-out-rabbits-and-goats-using-crispr-cas9-system
#13
Rihong Guo, Yongjie Wan, Dan Xu, Libin Cui, Mingtian Deng, Guomin Zhang, Ruoxin Jia, Wenjun Zhou, Zhen Wang, Kaiping Deng, Mingrui Huang, Feng Wang, Yanli Zhang
Myostatin (Mstn) is a conserved negative regulator of skeletal muscle mass in mammals. However, whether precise disruption of Mstn in livestock can be achieved and safely used to improve meat productivity has not been proven. We applied CRISPR/Cas9 system to generate Mstn knock-out (KO) rabbits and goats and then analyzed the changes in their phenotypes to answer this question. We efficiently generated 24 Mstn KO rabbits out of 32 newborn infants after embryo injection with two sgRNAs targeting rabbit Mstn, and found that the Mstn KO rabbits exhibited increased birthweight and a significantly increase in the weight ratios of the quadriceps and biceps muscles to the whole body...
2016: Scientific Reports
https://www.readbyqxmd.com/read/27412707/fermented-goat-milk-consumption-during-anaemia-recovery-ergogenic-effect-and-improvement-of-skeletal-muscle-homeostasis
#14
Jorge Moreno-Fernandez, Javier Diaz-Castro, Mario Pulido-Moran, Maria J M Alferez, Teresa Nestares, Inmaculada Lopez-Aliaga
PURPOSE: Anaemia is associated with fatigue and diminished muscular oxygenation, which may affect skeletal muscle (SM). No studies are available about the SM modifications during anaemia recovery; therefore, the aim of this study is to study SM homeostasis during anaemia recovery with fermented milks. METHODS: Forty male Wistar rats were placed on a pre-experimental period of 40 days, divided in two groups (control group receiving normal-Fe diet and Fe-deficient group receiving low-Fe diet)...
July 13, 2016: European Journal of Nutrition
https://www.readbyqxmd.com/read/27388698/seroprevalence-of-rift-valley-fever-virus-in-sheep-and-goats-in-zamb%C3%A3-zia-mozambique
#15
Anne-Lie Blomström, Isabelle Scharin, Hedvig Stenberg, Jaquline Figueiredo, Ofélia Nhambirre, Ana Abilio, Mikael Berg, José Fafetine
BACKGROUND: The Rift Valley fever virus (RVFV) is a vector-borne virus that causes disease in ruminants, but it can also infect humans. In humans, the infection can be asymptomatic but can also lead to illness, ranging from a mild disease with fever, headache and muscle pain to a severe disease with encephalitis and haemorrhagic fever. In rare cases, death can occur. In infected animals, influenza-like symptoms can occur, and abortion and mortality in young animals are indicative of RVFV infection...
2016: Infection Ecology & Epidemiology
https://www.readbyqxmd.com/read/27307997/comparison-of-myofibrillar-protein-degradation-antioxidant-profile-fatty-acids-metmyoglobin-reducing-activity-physicochemical-properties-and-sensory-attributes-of-gluteus-medius-and-infraspinatus-muscles-in-goats
#16
Kazeem D Adeyemi, Rafiat M Shittu, Azad B Sabow, Ahmed A Abubakar, Roselina Karim, Saiful A Karsani, Awis Q Sazili
BACKGROUND: The functionality of myofibrillar proteins is a major factor influencing the quality attributes of muscle foods. Nonetheless, the relationships between muscle type and oxidative changes in chevon during ageing are meagrely elucidated. Postmortem changes in antioxidant status and physicochemical properties of glycolytic gluteus medius (GM) and oxidative infraspinatus (IS) muscles in goats were compared. METHODS: Twenty Boer bucks (9-10 months old, body weight of 36...
2016: Journal of Animal Science and Technology
https://www.readbyqxmd.com/read/27276429/the-first-outbreak-of-taenia-ovis-infection-in-china
#17
Wangui Shi, Wei He, Xiaola Guo, Quanyuan Liu, Shengzhi Gao, Fang Zhan, Xu Liu, Yonghong Pan, Xuenong Luo, Yadong Zheng
Infection of Taenia ovis metacestodes in sheep or goats causes great economic losses due to condemnation of carcasses. T. ovis infection is not formally recorded in China to date. In October, 2015, T. ovis infection occurred in Jingtai County, China, and 113 of 192 sheep from one farm were infected. Cysts resided in the cardiac and skeletal muscle, and evaginated metacestodes had four suckers and scolex armed with approximately 23 hooks. Using cox1 and nad1 as molecular markers, the sample was further identified and the results showed that the cox1 and nad1 nucleotide sequences of the sample shared 99% identity with that of T...
October 2016: Parasitology International
https://www.readbyqxmd.com/read/27234532/white-snakeroot-poisoning-in-goats-variations-in-toxicity-with-different-plant-chemotypes
#18
T Z Davis, B L Stegelmeier, S T Lee, M G Collett, B T Green, J A Pfister, T J Evans, D S Grum, S Buck
Tremetone and possibly other benzofuran ketones are believed to be the toxic compounds in white snakeroot. However, disease has not been reproduced with purified toxins and the concentrations of the benzofuran ketones in white snakeroot populations that cause toxicosis have not been documented. The objectives of this study were to compare the toxicity of seven plant populations, better characterize the clinical and pathologic changes of poisoning, and correlate intoxication with benzofuran ketone content. Four of the seven white snakeroot collections were toxic at the dose and duration used in the study...
June 2016: Research in Veterinary Science
https://www.readbyqxmd.com/read/27149942/investigation-of-effect-of-fiber-orientation-on-mechanical-behavior-of-skeletal-muscle
#19
Chetan D Kuthe, Rashmi V Uddanwadiker
BACKGROUND: Existing studies have included little discussion of anisotropic and material non linearity of muscle tissue, and fiber orientation-based material properties of skeletal muscle tissue are not reported well in literature. There has been some dispute about material response of muscle in different fiber directions. It is necessary to have a better understanding of fiber orientation based material properties of skeletal muscle, to ensure the accuracy of computational models of muscle...
2016: Journal of Applied Biomaterials & Functional Materials
https://www.readbyqxmd.com/read/27138001/influence-of-diet-and-postmortem-ageing-on-oxidative-stability-of-lipids-myoglobin-and-myofibrillar-proteins-and-quality-attributes-of-gluteus-medius-muscle-in-goats
#20
Kazeem Dauda Adeyemi, Rafiat Morolayo Shittu, Azad Behnan Sabow, Mahdi Ebrahimi, Awis Qurni Sazili
This study appraised the effects of dietary blend of 80% canola oil and 20% palm oil and postmortem ageing on oxidative stability, fatty acids and quality attributes of gluteus medius (GM) muscle in goats. Twenty-four Boer bucks were randomly allotted to diet supplemented with 0, 4 and 8% oil blend, fed for 100 days and slaughtered, and the GM muscle was subjected to a 7 d chill storage (4±1°C). Diet had no effect (P> 0.05) on the colour, drip loss, thiobarbituric acid-reactive substances (TBARS) value, free thiol, carbonyl, myoglobin and metmyoglobin contents, metmyoglobin reducing activity (MRA), antioxidant enzyme activities and abundance of myosin heavy chain (MHC) and actin in the GM muscle in goats...
2016: PloS One
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