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https://www.readbyqxmd.com/read/28534278/the-effect-of-antioxidant-agents-addition-and-freezing-method-on-quality-parameters-of-frozen-thawed-ram-semen
#1
Leonidas Vichas, Ioannis A Tsakmakidis, Dimitrios Vafiadis, Georgios Tsousis, Eleni Malama, Constantin M Boscos
The aim of this study was to evaluate the effect of antioxidant agents and freezing methods on the ability of ram sperm to preserve its post-thaw quality characteristics. Six Chios rams were subjected to 52 weekly semen collections. Each ram was used as semen donor for freezing experiments once every 2 weeks. Equal number of good quality spermatozoa from each ejaculate (concentration ≥1 × 10(9) spermatozoa/ml, motility ≥70%, motility score ≥3.5) were pooled. Three equal aliquots of the pooled sample were diluted using three different fractions of a milk-based and glycerol extender (control, quercetin-enriched, α-tocopherol-enriched)...
May 22, 2017: Cell and Tissue Banking
https://www.readbyqxmd.com/read/28532835/effect-of-cholesterol-loaded-cyclodextrin-supplementation-on-tyrosine-phosphorylation-and-apoptosis-like-changes-in-frozen-thawed-hariana-bull-spermatozoa
#2
Hanuman P Yadav, Anuj Kumar, Nadeem Shah, Dharmendra S Chauhan, Atul Saxena, Sarvajeet Yadav, Dilip Kumar Swain
The beneficial effects of cholesterol loaded cyclodextrin (CLC) addition were evaluated in cryopreserved bull semen. Forty ejaculates were collected from Hariana bulls (n = 4), pooled and divided into 4 aliquots. All the aliquots were initially diluted in to egg yolk tris citrate and supplemented with CLC @ 0.5 mg (Group-II), 1.0 mg (G-III) and 2.0 mg (G-IV) CLC/120 × 10(6) spermatozoa or without CLC (G-I) that served as control. Extended semen was cryopreserved at -196 °C for 24 h. Seminal attributes like motility, viability, cryocapacitation like changes, tyrosine phosphorylation, apoptosis like changes in terms of mitochondrial transmembrane potential and DNA integrity were evaluated after equilibration and thawing...
July 1, 2017: Theriogenology
https://www.readbyqxmd.com/read/28531319/improved-sperm-kinematics-in-semen-samples-collected-after-2-h-versus-4-7-days-of-ejaculation-abstinence
#3
H Alipour, G Van Der Horst, O B Christiansen, F Dardmeh, N Jørgensen, H I Nielsen, C Hnida
STUDY QUESTION: Does a short abstinence period of only 2 h yield spermatozoa with better motility characteristics than samples collected after 4-7 days? SUMMARY ANSWER: Despite lower semen volume, sperm concentration, total sperm counts and total motile counts, higher percentages of motile spermatozoa with higher velocity and progressiveness were detected in samples obtained after 2 h. WHAT IS KNOWN ALREADY: Most studies that have assessed the effect of abstinence periods on sperm motility parameters in men with a sperm concentration below 15 million/ml have detected a higher percentage of motile spermatozoa in samples obtained after short abstinence periods...
May 22, 2017: Human Reproduction
https://www.readbyqxmd.com/read/28525330/effect-of-boar-ejaculate-fraction-extender-type-and-time-of-storage-on-quality-of-spermatozoa
#4
A Dziekońska, K Świąder, M Koziorowska-Gilun, K Mietelska, Ł Zasiadczyk, W Kordan
The aim of this study was to investigate the effect the sperm-rich fraction (F1) and the post-F1 fraction (F2) on the quality of boar spermatozoa stored in a liquid state. Ejaculates were collected from three Polish Landrace boars. Each ejaculate fraction was diluted with BTS short-term extender and Safe-Cell Plus (SCP) long-term extender and stored for seven days (D1-D7) at 17°C. Analyses included sperm motility parameters, normal apical ridge (NAR) acrosomes and plasma membrane integrity (PMI). Prior to the dilution of fractions, marked changes (p<0...
March 28, 2017: Polish Journal of Veterinary Sciences
https://www.readbyqxmd.com/read/28521061/extracellular-camp-activates-molecular-signalling-pathways-associated-with-sperm-capacitation-in-bovines
#5
Carlos Agustín I Alonso, Claudia E Osycka-Salut, Luciana Castellano, Andreína Cesari, Nicolás Di Siervi, Adrián Mutto, Anders Johannisson, Jane M Morrell, Carlos Davio, Silvina Perez-Martinez
STUDY QUESTION: Is extracellular cAMP involved in the regulation of signalling pathways in bovine sperm capacitation? SUMMARY ANSWER: Extracellular cAMP-induced sperm capacitation through the activation of different signalling pathways that involve phospholipase C (PLC), PKC/ERK1-2 signalling and an increase in sperm Ca2+ levels, as well as soluble AC and cAMP/protein kinase A (PKA) signalling. WHAT IS KNOWN ALREADY: In order to fertilize the oocyte, ejaculated spermatozoa must undergo a series of changes in the female reproductive tract, known as capacitation...
May 18, 2017: Molecular Human Reproduction
https://www.readbyqxmd.com/read/28520723/follicular-size-predicts-success-in-artificial-insemination-with-frozen-thawed-sperm-in-donkeys
#6
Joseph Saragusty, Alemayehu Lemma, Thomas Bernd Hildebrandt, Frank Göritz
In asses, semen collection, cryopreservation, and artificial insemination (AI) with frozen-thawed semen have been scarcely described and success rate, particularly following AI, is reportedly low. In the absence of reliable protocols, assisted reproductive technologies cannot support the conservation efforts aimed at endangered wild ass species and domestic donkey breeds. Two experiments were conducted in this study. In experiment 1 we evaluated freezing Abyssinian donkey (N = 5, 4 ejaculates each) spermatozoa using three freezing extenders (Berliner Cryomedium + glycerol, BC+G; BotuCrio, BOTU; INRAFreeze, INRA) and two cryopreservation techniques (liquid nitrogen vapour, LNV; directional freezing, DF)...
2017: PloS One
https://www.readbyqxmd.com/read/28517368/testicular-thermoregulation-scrotal-surface-temperature-patterns-and-semen-quality-of-water-buffalo-bulls-reared-in-a-tropical-climate
#7
L K X Silva, J S Sousa, A O A Silva, J B Lourenço Junior, C Faturi, L G Martorano, I M Franco, M H A Pantoja, D V Barros, A R Garcia
This study evaluated the capacity of thermoregulation and its consequences on the scrotal surface temperature patterns and semen quality of buffalo bulls raised in a wet tropical climate. Eleven water buffaloes were evaluated in the rainiest, in the transitional and in the less rainy season. Air temperature and humidity were consistently high, but the animals did not show thermal stress in any season. The scrotal temperature gradient of buffalo bulls using infrared thermography was described, and three parallel and decreasing thermal bands were characterised...
May 18, 2017: Andrologia
https://www.readbyqxmd.com/read/28517043/sperm-chromatin-quality-and-dna-integrity-in-partial-versus-total-globozoospermia
#8
A R Talebi, J Ghasemzadeh, M A Khalili, I Halvaei, F Fesahat
Globozoospermia is a severe form of teratozoospermia with low incidence in infertile patients, considered as one of the important causes of male infertility. The objective was to investigate the chromatin/DNA integrity as well as apoptosis in ejaculated spermatozoa of cases with partial or total globozoospermia. Fifty-seven semen samples were divided into three groups of partial globozoospermia (n = 17), total globozoospermia (n = 10) and normozoospermia (control; n = 30). Sperm chromatin condensation, DNA integrity and apoptosis were assessed using cytochemical assays...
May 18, 2017: Andrologia
https://www.readbyqxmd.com/read/28514992/sexual-difficulties-faced-by-men-in-the-solomon-islands-a-mixed-methods-study
#9
Paraniala Silas C Lui, Michael P Dunne, Philip Baker, Verzilyn Isom
Background: To date there has been little research into men's sexual and reproductive health in Pacific Island countries. The aim of this study was to describe men's sexual difficulties and barriers to their seeking reproductive health care in the Solomon Islands. Methods: The study included qualitative inquiry (17 individual interviews and three focus group discussions with a total of 21 men) and a quantitative quasi-randomised quota sample household survey (n=400). The prevalence of sexual difficulties and potential risk factors, such as chronic diseases, health risk behaviours, depression and psychological distress were measured using standardised questions translated into pidgin...
May 18, 2017: Sexual Health
https://www.readbyqxmd.com/read/28511862/effect-of-different-concentrations-of-egg-yolk-and-virgin-coconut-oil-in-tris-based-extenders-on-chilled-and-frozen-thawed-bull-semen
#10
A A Tarig, H Wahid, Y Rosnina, N Yimer, Y M Goh, F H Baiee, A M Khumran, H Salman, M Ebrahimi
The aim of this study was to evaluate the effects of 8% virgin coconut oil (VCO) combined with different percentages of egg yolk in Tris extender on the quality of chilled and frozen-thawed bull semen. A total of 24 ejaculates from four bulls were collected using an electroejaculator. Semen samples were diluted with 8% VCO in Tris extender which contained different concentrations 0% (control), 4%, 8%, 12%, 16% and 20% egg yolk. The diluted semen samples were divided into two fractions: one was chilled and stored at 4°C until evaluation after 24, 72, and 144h; the second fraction was processed by chilling for 3h at 4°C to equilibrate, then packaged in 0...
April 10, 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/28508664/aphrodisiac-activity-of-the-aqueous-crude-extract-of-purple-corn-zea-mays-in-male-rats
#11
Miguel Carro-Juárez, Magdalena G Rodríguez-Santiago, Miguel Angel Franco, María Eugenia Hueletl-Soto
In the present study, the aphrodisiac properties of the purple corn ( Zea mays) in male rats were analyzed. The aqueous crude extract of purple corn (at 25, 50, and 75 mg/kg) was administered to ( a) copulating male rats and ( b) anesthetized and spinal cord transected male rats. Behavioral parameters of copulatory behavior and parameters of the genital motor pattern of ejaculation previous to its inhibition, under the influence of the purple corn extract, are described. Administration of the aqueous crude extract of purple corn significantly facilitates the arousal and execution of male rat sexual behavior without significant influences on the ambulatory behavior...
January 1, 2017: Journal of Evidence-based Complementary & Alternative Medicine
https://www.readbyqxmd.com/read/28508598/for-debate-should-we-worry-about-hematospermia-in-adolescents-and-young-men-a-report-of-six-cases-and-review-of-the-literature
#12
EDITORIAL
Vincenzo De Sanctis, Javaad Zargooshi, Alberto Marsciani, Ashraf T Soliman, Heba Elsedfy, Nada A Soliman, Salvatore Di Maio
Hematospermia or hemospermia is defined as the presence of blood in ejaculate. The true prevalence of the condition is unknown because many cases escape the patient's notice, and remain unrecognized and unreported. There are two main aims in the patient evaluation: first, to ensure that there is no specific condition that is treatable; second, to reassure the patient's parents that no causative factor is present. Many physicians are unfamiliar with this disorder and this forms the basis for our current review...
March 2017: Pediatric Endocrinology Reviews: PER
https://www.readbyqxmd.com/read/28507884/a-retrospective-analysis-of-complications-associated-with-bone-morphogenetic-protein-2-in-anterior-lumbar-interbody-fusion
#13
Kevork Hindoyan, Justin Tilan, Zorica Buser, Jeremiah R Cohen, Darrel S Brodke, Jim A Youssef, Jong-Beom Park, S Tim Yoon, Hans-Joerg Meisel, Jeffrey C Wang
STUDY DESIGN: Retrospective review. OBJECTIVE: The aim of our study was to quantify the frequency of complications associated with recombinant human bone morphogenetic protein 2 (rhBMP-2) use in anterior lumbar interbody fusion (ALIF). METHODS: The orthopedic subset of the Medicare database (PearlDiver) was queried for this retrospective cohort study using International Statistical Classification of Diseases 9 (ICD-9) and Current Procedure Terminology (CPT) codes for ALIF procedures with and without rhBMP-2 between 2005 and 2010...
April 2017: Global Spine Journal
https://www.readbyqxmd.com/read/28507189/oxidative-stress-affects-sperm-performance-and-ejaculate-redox-status-in-subordinate-house-sparrows
#14
Alfonso Rojas Mora, Alexandra Firth, Sophie Blareau, Armelle Vallat, Fabrice Helfenstein
Oxidative stress (OS) is the result of random cellular damage caused by reactive oxygen species that leads to cell death, ageing, or illness. Most physiological processes can result in OS, which in turn has been identified as a major cause of infertility. In promiscuous species, the fertilizing ability of the ejaculate partly determines the male reproductive success. When dominance determines access to fertile females, theory predicts that lower ranking males should increase resource investment into enhancing ejaculate quality...
May 15, 2017: Journal of Experimental Biology
https://www.readbyqxmd.com/read/28505937/re-does-abstinence-from-ejaculation-before-prostate-mri-improve-evaluation-of-the-seminal-vesicles
#15
Cary Siegel
No abstract text is available yet for this article.
June 2017: Journal of Urology
https://www.readbyqxmd.com/read/28505932/re-rasch-analysis-of-the-premature-ejaculation-diagnostic-tool-pedt-and-the-international-index-of-erectile-function-iief-in-an-iranian-sample-of-prostate-cancer-patients
#16
https://www.readbyqxmd.com/read/28501790/obesity-aggravates-toxic-effect-of-bpa-on-spermatogenesis
#17
Weiyue Hu, Tianyu Dong, Lingling Wang, Quanquan Guan, Ling Song, Daozhen Chen, Zuomin Zhou, Minjian Chen, Yankai Xia, Xinru Wang
Both bisphenol A (BPA) and obesity affect male reproductive system. However, whether there is an interaction between them remains poorly understood. The aim of the present study was to evaluate the interaction between BPA exposure and obesity on semen quality and elucidate the mechanism in humans and animals. We firstly analyzed the interaction on semen volume, sperm count per ejaculate, sperm concentration and sperm motility in 357 men, and found that urinary BPA concentration was significantly correlated with sperm count per ejaculate in obese men (β=-34...
May 11, 2017: Environment International
https://www.readbyqxmd.com/read/28501545/betamethasone-causes-intergenerational-reproductive-impairment-in-male-rats
#18
Cibele Dos Santos Borges, Taina Louise Pacheco, Katiussia Pinho da Silva, Fábio Henrique Fernandes, Mary Gregory, André Sampaio Pupo, Daisy Maria F Salvadori, Daniel G Cyr, Wilma De G Kempinas
Prenatal betamethasone (BM) exposure in rats negatively impacts sperm quality and male fertility. Studies have shown that BM can cause multi-generational effects on the pituitary-adrenal-axis of rats. The objective of this study was to assess the reproductive development and fertility of male rats (F2) whose fathers (F1) were exposed to BM (0.1mg/kg) on gestational days 12, 13, 18 and 19. In F2 rats, there was a significant reduction in body weights of the BM-treated group at PND 1 as well as delayed onset of puberty, and decreases in FSH levels, Leydig cell volume, sperm number and motility, seminal vesicle contractility and ejaculated volume...
May 10, 2017: Reproductive Toxicology
https://www.readbyqxmd.com/read/28497478/comparison-of-the-clinical-efficacy-and-safety-of-the-on-demand-use-of-paroxetine-dapoxetine-sildenafil-and-combined-dapoxetine-with-sildenafil-in-treatment-of-patients-with-premature-ejaculation-a-randomised-placebo-controlled-clinical-trial
#19
M Abu El-Hamd, A Abdelhamed
The aim of the study was to compare the clinical efficacy and safety of the on-demand use of paroxetine, dapoxetine, sildenafil and combined dapoxetine with sildenafil in treatment of patients with premature ejaculation (PE). In a single-blind placebo-controlled clinical study, 150 PE patients without erectile dysfunction (ED) were included during the period of March 2015 to May 2016. Patients were randomly divided into five groups (30 patients each). On demand placebo, paroxetine (30 mg), dapoxetine (30 mg), sildenafil citrate (50 mg) and combined dapoxetine (30 mg) with sildenafil citrate (50 mg) were given for patients for 6 weeks in each group respectively...
May 12, 2017: Andrologia
https://www.readbyqxmd.com/read/28497461/icsi-outcome-in-patients-with-high-dna-fragmentation-testicular-versus-ejaculated-spermatozoa
#20
M Arafa, A AlMalki, M AlBadr, H Burjaq, A Majzoub, S AlSaid, H Elbardisi
Sperm DNA fragmentation (SDF) has emerged as an important biomarker in the assessment of male fertility potential with contradictory results regarding its effect on ICSI. The aim of this study was to evaluate intracytoplasmic sperm injection (ICSI) outcomes in male patients with high SDF using testicular versus ejaculated spermatozoa. This is a prospective study on 36 men with high-SDF levels who had a previous ICSI cycle from their ejaculates. A subsequent ICSI cycle was performed using spermatozoa retrieved through testicular sperm aspiration...
May 12, 2017: Andrologia
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