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myoblast transplantation MDX

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https://www.readbyqxmd.com/read/26941360/induction-of-ccaat-enhancer-binding-protein-%C3%AE-expression-with-the-phosphodiesterase-inhibitor-isobutylmethylxanthine-improves-myoblast-engraftment-into-dystrophic-muscle
#1
Neena Lala-Tabbert, Dechen Fu, Nadine Wiper-Bergeron
UNLABELLED: Duchenne muscular dystrophy (DMD), caused by mutations in the dystrophin gene, is the most common muscular dystrophy. Characterized by rounds of muscle degeneration and regeneration, DMD features progressive muscle wasting and is fatal. One approach for treatment is transplantation of muscle progenitor cells to repair and restore dystrophin expression to damaged muscle. However, the success of this approach has been limited by difficulties in isolating large numbers of myogenic progenitors with strong regenerative potential, poor engraftment, poor survival of donor cells, and limited migration in the diseased muscle...
April 2016: Stem Cells Translational Medicine
https://www.readbyqxmd.com/read/26459029/laminin-therapy-for-the-promotion-of-muscle-regeneration
#2
REVIEW
Ingo Riederer, Adriana Cesar Bonomo, Vincent Mouly, Wilson Savino
Muscle regeneration is essentially due to activation of satellite cells, which can be isolated and amplified ex vivo, thus representing good candidates for cell therapy. Accumulating data show that the local microenvironment plays a major role during muscle regeneration. In the satellite cell niche, a major extracellular matrix protein is laminin. Human myoblasts transplanted into immunodeficient mice are preferentially located in laminin-enriched areas. Additionally, laminin-111 enhances myoblast proliferation in vitro and increases expression of the α7β1 integrin-type laminin receptor...
November 14, 2015: FEBS Letters
https://www.readbyqxmd.com/read/26223276/mmp1-gene-expression-enhances-myoblast-migration-and-engraftment-following-implanting-into-mdx-scid-mice
#3
Haiying Pan, Kinga Vojnits, Thomas T Liu, Fanwei Meng, Lei Yang, Yigang Wang, Johnny Huard, Charles S Cox, Kevin P Lally, Yong Li
Myoblast transplantation (MT) is a method to introduce healthy genes into abnormal skeletal muscle. It has been considered as a therapeutic modality in the last few decades for diseases such as Duchenne Muscular Dystrophy (DMD). However, challenges including cell death and poor graft engraftment have limited its application. The current experiment utilizes MMP1 gene transfer to improve the efficacy of myoblast transplantation into the diseased dystrophic skeletal muscle of mdx mice. Our results indicated that MMP1 expression can promote myogenic differentiation and fusion capacities, increase migration of MMP1 expressing myoblasts in vitro, as well as improve engraftment of dystrophin positive myofibers in vivo...
2015: Cell Adhesion & Migration
https://www.readbyqxmd.com/read/24919771/options-for-tracking-gfp-labeled-transplanted-myoblasts-using-in-vivo-fluorescence-imaging-implications-for-tracking-stem-cell-fate
#4
Zhong Yang, Yaming Wang, Yanan Li, Qiang Liu, Qing Zeng, Xiaoyin Xu
BACKGROUND: Green fluorescent protein (GFP) is a useful biomarker, widely used in biomedical research to track stem cells after transplantation and/or to assess therapeutic transgene expression. However, both GFP and therapeutic gene products themselves may be immunogenic to the recipient. The main aim of this study was to use animal models to evaluate potential impact of GFP on the cell engraftment and to optimize tracking strategies prior to transplantation. RESULTS: By using a fluorescent imaging (FLI) system, we investigated the dynamic cell behavior of GFP-transduced myoblasts in tibialis anterior (TA) muscles of immunocompetent mdx mice and immuno-compromised nude mice over a period of three months...
2014: BMC Biotechnology
https://www.readbyqxmd.com/read/23977226/matrix-metalloproteinase-9-inhibition-improves-proliferation-and-engraftment-of-myogenic-cells-in-dystrophic-muscle-of-mdx-mice
#5
Sajedah M Hindi, Jonghyun Shin, Yuji Ogura, Hong Li, Ashok Kumar
Duchenne muscular dystrophy (DMD) caused by loss of cytoskeletal protein dystrophin is a devastating disorder of skeletal muscle. Primary deficiency of dystrophin leads to several secondary pathological changes including fiber degeneration and regeneration, extracellular matrix breakdown, inflammation, and fibrosis. Matrix metalloproteinases (MMPs) are a group of extracellular proteases that are involved in tissue remodeling, inflammation, and development of interstitial fibrosis in many disease states. We have recently reported that the inhibition of MMP-9 improves myopathy and augments myofiber regeneration in mdx mice (a mouse model of DMD)...
2013: PloS One
https://www.readbyqxmd.com/read/23568004/molecular-imaging-to-target-transplanted-muscle-progenitor-cells
#6
Kelly Gutpell, Rebecca McGirr, Lisa Hoffman
Duchenne muscular dystrophy (DMD) is a severe genetic neuromuscular disorder that affects 1 in 3,500 boys, and is characterized by progressive muscle degeneration. In patients, the ability of resident muscle satellite cells (SCs) to regenerate damaged myofibers becomes increasingly inefficient. Therefore, transplantation of muscle progenitor cells (MPCs)/myoblasts from healthy subjects is a promising therapeutic approach to DMD. A major limitation to the use of stem cell therapy, however, is a lack of reliable imaging technologies for long-term monitoring of implanted cells, and for evaluating its effectiveness...
2013: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/23526927/neuromuscular-electrical-stimulation-as-a-method-to-maximize-the-beneficial-effects-of-muscle-stem-cells-transplanted-into-dystrophic-skeletal-muscle
#7
Giovanna Distefano, Ricardo Jose Ferrari, Christopher Weiss, Bridget M Deasy, Michael L Boninger, G Kelley Fitzgerald, Johnny Huard, Fabrisia Ambrosio
Cellular therapy is a potential approach to improve the regenerative capacity of damaged or diseased skeletal muscle. However, its clinical use has often been limited by impaired donor cell survival, proliferation and differentiation following transplantation. Additionally, functional improvements after transplantation are all-too-often negligible. Because the host microenvironment plays an important role in the fate of transplanted cells, methods to modulate the microenvironment and guide donor cell behavior are warranted...
2013: PloS One
https://www.readbyqxmd.com/read/23056408/macrophages-improve-survival-proliferation-and-migration-of-engrafted-myogenic-precursor-cells-into-mdx-skeletal-muscle
#8
Pierre-François Lesault, Marine Theret, Mélanie Magnan, Sylvain Cuvellier, Yiming Niu, Romain K Gherardi, Jacques P Tremblay, Luc Hittinger, Bénédicte Chazaud
Transplantation of muscle precursor cells is of therapeutic interest for focal skeletal muscular diseases. However, major limitations of cell transplantation are the poor survival, expansion and migration of the injected cells. The massive and early death of transplanted myoblasts is not fully understood although several mechanisms have been suggested. Various attempts have been made to improve their survival or migration. Taking into account that muscle regeneration is associated with the presence of macrophages, which are helpful in repairing the muscle by both cleansing the debris and deliver trophic cues to myoblasts in a sequential way, we attempted in the present work to improve myoblast transplantation by coinjecting macrophages...
2012: PloS One
https://www.readbyqxmd.com/read/22990676/myoblasts-derived-from-normal-hescs-and-dystrophic-hipscs-efficiently-fuse-with-existing-muscle-fibers-following-transplantation
#9
Sébastien Goudenege, Carl Lebel, Nicolas B Huot, Christine Dufour, Isao Fujii, Jean Gekas, Joël Rousseau, Jacques P Tremblay
Human embryonic stem cells (hESCs) and human-induced pluripotent stem cells (hiPSCs) have an endless self-renewal capacity and can theoretically differentiate into all types of lineages. They thus represent an unlimited source of cells for therapies of regenerative diseases, such as Duchenne muscular dystrophy (DMD), and for tissue repair in specific medical fields. However, at the moment, the low number of efficient specific lineage differentiation protocols compromises their use in regenerative medicine. We developed a two-step procedure to differentiate hESCs and dystrophic hiPSCs in myogenic cells...
November 2012: Molecular Therapy: the Journal of the American Society of Gene Therapy
https://www.readbyqxmd.com/read/22764051/hypoxia-promotes-satellite-cell-self-renewal-and-enhances-the-efficiency-of-myoblast-transplantation
#10
Weiyi Liu, Yefei Wen, Pengpeng Bi, Xinsheng Lai, X Shawn Liu, Xiaoqi Liu, Shihuan Kuang
Microenvironmental oxygen (O(2)) regulates stem cell activity, and a hypoxic niche with low oxygen levels has been reported in multiple stem cell types. Satellite cells are muscle-resident stem cells that maintain the homeostasis and mediate the regeneration of skeletal muscles. We demonstrate here that hypoxic culture conditions favor the quiescence of satellite cell-derived primary myoblasts by upregulating Pax7, a key regulator of satellite cell self-renewal, and downregulating MyoD and myogenin. During myoblast division, hypoxia promotes asymmetric self-renewal divisions and inhibits asymmetric differentiation divisions without affecting the overall rate of proliferation...
August 2012: Development
https://www.readbyqxmd.com/read/22449443/administration-of-a-soluble-activin-type-iib-receptor-promotes-the-transplantation-of-human-myoblasts-in-dystrophic-mice
#11
Raouia Fakhfakh, Se-Jin Lee, Jacques P Tremblay
Duchenne muscular dystrophy (DMD) is a recessive disease caused by a dystrophin gene mutation. Myoblast transplantation permits the introduction of the dystrophin gene into dystrophic muscle fibers. However, this strategy has so far produced limited results. Modulation of transforming growth factor-β (TGF-β) superfamily signaling promotes skeletal muscle differentiation and growth and myogenic regeneration. We investigated the possibility that the combination of TGF-β superfamily signaling inhibition with myoblast transplantation might be an effective therapeutic approach in dystrophin-deficient patients...
2012: Cell Transplantation
https://www.readbyqxmd.com/read/22183053/alterations-in-the-expression-of-leukemia-inhibitory-factor-following-exercise-comparisons-between-wild-type-and-mdx-muscles
#12
Liam C Hunt, Chantal Anthea Coles, Christopher M Gorman, Elizabeth M Tudor, Gayle M Smythe, Jason D White
BACKGROUND: Leukemia inhibitory factor (LIF) is a pleiotropic cytokine, belonging to the interleukin-6 family of cytokines, that has been suggested to have positive effects on myogenesis following injury and to minimise dystrophic pathology in mdx mice. Previous reports have suggested that Lif mRNA is up-regulated in the limb and diaphragm muscles of mdx mice, in human cases of dystrophy and acutely following exercise. This study examined expression of Lif mRNA in the quadriceps muscles of mdx and wild-type mice that were either sedentary or allowed to exercise voluntarily for two weeks...
2011: PLoS Currents
https://www.readbyqxmd.com/read/21903674/alpha-sarcoglycan-is-required-for-fgf-dependent-myogenic-progenitor-cell-proliferation-in-vitro-and-in-vivo
#13
Marco Cassano, Arianna Dellavalle, Francesco Saverio Tedesco, Mattia Quattrocelli, Stefania Crippa, Flavio Ronzoni, Agnese Salvade, Emanuele Berardi, Yvan Torrente, Giulio Cossu, Maurilio Sampaolesi
Mice deficient in α-sarcoglycan (Sgca-null mice) develop progressive muscular dystrophy and serve as a model for human limb girdle muscular dystrophy type 2D. Sgca-null mice suffer a more severe myopathy than that of mdx mice, the model for Duchenne muscular dystrophy. This is the opposite of what is observed in humans and the reason for this is unknown. In an attempt to understand the cellular basis of this severe muscular dystrophy, we isolated clonal populations of myogenic progenitor cells (MPCs), the resident postnatal muscle progenitors of dystrophic and wild-type mice...
October 2011: Development
https://www.readbyqxmd.com/read/21854253/human-muscle-derived-cell-populations-isolated-by-differential-adhesion-rates-phenotype-and-contribution-to-skeletal-muscle-regeneration-in-mdx-scid-mice
#14
Steven M Chirieleison, Joseph M Feduska, Rebecca C Schugar, Yuko Askew, Bridget M Deasy
Muscle-derived stem cells (MDSCs) isolated from murine skeletal tissue by the preplate method have displayed the capability to commit to the myogenic lineage and regenerate more efficiently than myoblasts in skeletal and cardiac muscle in murine Duchenne Muscular Dystrophy mice (mdx). However, until now, these studies have not been translated to human muscle cells. Here, we describe the isolation, by a preplate technique, of candidate human MDSCs, which exhibit myogenic and regenerative characteristics similar to their murine counterparts...
February 2012: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/21689628/follistatin-improves-skeletal-muscle-healing-after-injury-and-disease-through-an-interaction-with-muscle-regeneration-angiogenesis-and-fibrosis
#15
Jinhong Zhu, Yong Li, Aiping Lu, Burhan Gharaibeh, Jianqun Ma, Tetsuo Kobayashi, Andres J Quintero, Johnny Huard
Recovery from skeletal muscle injury is often incomplete because of the formation of fibrosis and inadequate myofiber regeneration; therefore, injured muscle could benefit significantly from therapies that both stimulate muscle regeneration and inhibit fibrosis. To this end, we focused on blocking myostatin, a member of the transforming growth factor-β superfamily and a negative regulator of muscle regeneration, with the myostatin antagonist follistatin. In vivo, follistatin-overexpressing transgenic mice underwent significantly greater myofiber regeneration and had less fibrosis formation compared with wild-type mice after skeletal muscle injury...
August 2011: American Journal of Pathology
https://www.readbyqxmd.com/read/21535912/losartan-enhances-the-success-of-myoblast-transplantation
#16
Raouia Fakhfakh, Yann Lamarre, Daniel Skuk, Jacques P Tremblay
Duchenne muscular dystrophy is a recessive X-linked genetic disease caused by dystrophin gene mutations. Cell therapy can be a potential approach aiming to introduce a functional dystrophin in the dystrophic patient myofibers. However, this strategy produced so far limited results. Transforming growth factor-β (TGF-β) is a negative regulator of skeletal muscle development and is responsible for limiting myogenic regeneration. The combination of TGF-β signaling inhibition with myoblast transplantation can be an effective therapeutic approach in dystrophin-deficient patients...
2012: Cell Transplantation
https://www.readbyqxmd.com/read/21535909/fibrin-gel-improves-the-survival-of-transplanted-myoblasts
#17
Catherine Gerard, Marie Anne Forest, Genevieve Beauregard, Daniel Skuk, Jacques P Tremblay
Duchenne muscular dystrophy (DMD) is the most frequent muscular dystrophy in children and young adults. Currently, there is no cure for the disease. The transplantation of healthy myoblasts is an experimental therapeutic strategy, since it could restore the expression of dystrophin in DMD muscles. Nevertheless, this cellular therapy is limited by immune reaction, low migration of the implanted cells, and high early cell death that could be at least partially due to anoikis. To avoid the lack of attachment of the cells to an extracellular matrix after the transplantation, which is the cause of anoikis, we tested the use of a fibrin gel for myoblast transplantation...
2012: Cell Transplantation
https://www.readbyqxmd.com/read/21408080/contribution-of-human-muscle-derived-cells-to-skeletal-muscle-regeneration-in-dystrophic-host-mice
#18
Jinhong Meng, Carl F Adkin, Shi-wen Xu, Francesco Muntoni, Jennifer E Morgan
BACKGROUND: Stem cell transplantation is a promising potential therapy for muscular dystrophies, but for this purpose, the cells need to be systemically-deliverable, give rise to many muscle fibres and functionally reconstitute the satellite cell niche in the majority of the patient's skeletal muscles. Human skeletal muscle-derived pericytes have been shown to form muscle fibres after intra-arterial transplantation in dystrophin-deficient host mice. Our aim was to replicate and extend these promising findings...
2011: PloS One
https://www.readbyqxmd.com/read/21355312/-co-transplantation-of-myoblasts-and-schwann-cells-in-the-therapy-of-duchenne-muscular-dystrophy
#19
Li Luo, Hong-yu Zhou
OBJECTIVE: To study the fusion of transplanted myoblast with host muscle cells and the expression of dystrophin in the muscle of mdx mice model for Duchenne muscular dystrophy (DMD) after local co-transplantation of myoblasts and schwann cells. METHODS: Myoblasts and schwann cells were prepared with gastrocnemius muscle taken from newborn C57BL/10 mice. Twenty four mdx mice were divided into two groups, one group received the injection of myoblasts into gastrocnemius muscle of left behind limb of mdx mice, another group received the cotransplantation of schwann cells and myoblasts in a ratio of 1:10...
January 2011: Sichuan da Xue Xue Bao. Yi Xue Ban, Journal of Sichuan University. Medical Science Edition
https://www.readbyqxmd.com/read/21192317/the-survival-of-myoblasts-after-intramuscular-transplantation-is-improved-when-fewer-cells-are-injected
#20
Kathryn L Pellegrini, Manfred W Beilharz
BACKGROUND: Myoblast transplantation has long been studied as a potential therapy for Duchenne muscular dystrophy as the incorporation of donor myoblasts into host muscle allows the production of functional dystrophin protein. However, the clinical feasibility of this approach is limited by the poor survival of the donor cells in the weeks after transplantation. It has recently been determined that the intramuscular transplantation of large numbers of cells can lead to the formation of ischemic necrosis in the center of these cell masses...
March 15, 2011: Transplantation
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