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https://www.readbyqxmd.com/read/29031244/development-of-new-recombinant-dgk-antigen-for-diagnosis-of-dirofilaria-immitis-infections-in-dogs-using-elisa-technique-and-its-comparison-to-molecular-methods
#1
Rahmat Solgi, Seyed Mahmoud Sadjjadi, Mehdi Mohebali, Zabihollah Zarei, Majid Golkar, Abbasali Raz
Background: Dirofilaria immitis is a cosmopolitan zoonotic, vector-borne parasite of carnivorous animals causing dirofilariasis in human beings. Common commercial serodiagnostic tests for canine dirofilariasis usually lead to different results in their sensitivity and specificity. The present study reports development of recombinant DgK (rDgK) antigen of D. immitis for accurate immunodiagnosis of D. Immitis-infected dogs using indirect ELISA test. Methods: The rDgK coding sequence was successfully sequenced, codon optimized and cloned in pET-24a(+) expression vector and then expressed in Escherichia coli...
October 15, 2017: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/29031179/carbosilane-metallodendrimers-based-on-copper-ii-complexes-synthesis-epr-characterization-and-anticancer-activity
#2
Natalia Sanz Del Olmo, Marta Maroto-Díaz, Rafael Gómez, Paula Ortega, Michela Cangiotti, M Francesca Ottaviani, F Javier de la Mata
A series of new organometallic carbosilane dendrimers functionalized with Copper(II) complex on the surface were synthesized and characterized as potential anticancer agents. These metallodendrimers were synthesized through the reaction of dendritic ligands containing N,N- and N,O- donor atoms able to act as chelating agents with CuCl2 as metallic ion precursor. The structural characterization of these complexes was addressed through the use of different analytical and spectroscopical techniques. Particularly, an electron paramagnetic resonance study was performed to corroborate the coordination properties of these dendritic ligands...
September 27, 2017: Journal of Inorganic Biochemistry
https://www.readbyqxmd.com/read/29031159/development-of-fingermarks-on-latex-gloves-the-solution-to-a-challenging-surface
#3
Tomer Arbeli, Yakir Liptz, Ravell Bengiat, Michal Levin-Elad
Used Latex gloves found at crime scenes can provide strong evidence against a suspect as they almost certainly contain both the fingermarks and DNA of the perpetrator who had worn them. However, over the years, Latex gloves have proved to be a rather difficult substrate for fingermarks development, with most of the standard techniques producing poor results. In this study, the two main protocols for development on either porous or non-porous surfaces: Ninhydrin-HFE and superglue fuming followed by crystal violet (CV) dyeing, respectively, had been examined on 100 disposable Latex gloves from twenty five donors...
September 28, 2017: Forensic Science International
https://www.readbyqxmd.com/read/29031155/the-effect-of-nanoparticle-aggregation-on-surfactant-foam-stability
#4
Zuhair A AlYousef, Mohammed A Almobarky, David S Schechter
The combination of nanoparticles (NPs) and surfactant may offer a novel technique of generating stronger foams for gas mobility control. This study evaluates the potential of silica NPs to enhance the foam stability of three nonionic surfactants. Results showed that the concentration of surfactant and NPs is a crucial parameter for foam stability and that there is certain concentrations for strong foam generation. A balance in concentration between the nonionic surfactants and the NPs can enhance the foam stability as a result of forming flocs in solutions...
September 20, 2017: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/29031149/highly-efficient-photocatalysis-toward-tetracycline-of-nitrogen-doped-carbon-quantum-dots-sensitized-bismuth-tungstate-based-on-interfacial-charge-transfer
#5
Jin Zhang, Xingzhong Yuan, Longbo Jiang, Zhibin Wu, Xiaohong Chen, Hou Wang, Hui Wang, Guangming Zeng
In this study, a novel N-CQDs/Bi2WO6 was synthesized through a facile hydrothermal method. Multiple techniques were applied to investigate the structures, morphologies, optical and electronic properties and photocatalytic performance of as-prepared samples. The results indicated that the hybrid materials were formed with N-CQDs attached on the surface of sphere-like Bi2WO6. The photocatalytic activity of N-CQDs/Bi2WO6 materials was evaluated sufficiently by using tetracycline (TC) as target organic pollutant...
September 22, 2017: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/29031111/immobilization-of-sugars-in-supermacroporous-cryogels-for-the-purification-of-lectins-by-affinity-chromatography
#6
Gabriel Ramos Ferreira Gonçalves, Olga Reinert Ramos Gandolfi, Leandro Soares Santos, Renata Cristina Ferreira Bonomo, Cristiane Martins Veloso, Lizzy Ayra Alcântara Veríssimo, Rafael da Costa Ilhéu Fontan
Lectins are glycoproteins that bind to carbohydrates or glycoconjugates by specific interactions. The specificity of lectins to various carbohydrates is a determinant factor in the choice of ligand for the chromatographic matrix when using chromatography as a lectin purification technique. In this work, the immobilization of three different aminated carbohydrates on the surface of macroporous polymeric cryogels was evaluated. Carbohydrates were immobilized on cryogel surfaces via the glutaraldehyde method to create spacer arms, reducing steric hindrance...
October 9, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/29031107/accuracy-precision-and-inter-rater-reliability-of-micro-ct-analysis-of-false-starts-on-bones-a-preliminary-validation-study
#7
Guido Pelletti, Giovanni Cecchetto, Alessia Viero, Paolo Fais, Michael Weber, Diego Miotto, Massimo Montisci, Guido Viel, Chiara Giraudo
Micro computed tomography (micro-CT) has already been proposed as a useful technique for the qualitative analysis of false starts (FS) produced on human bones, although the reliability and the error rate of this technique have not been tested yet, neither for qualitative nor for quantitative assessments. The aim of the present study was to test the morphological agreement, accuracy, precision and inter-rater reliability of micro-CT analysis of FS on bones. The morphological agreement was assessed through the degree of concordance among the 3 independent blind raters in the identification of the shape of 24 FS manually produced on bones by 3 different saws (8 FS for each saw)...
October 6, 2017: Legal Medicine
https://www.readbyqxmd.com/read/29031099/shear-wave-elastography-for-characterization-of-breast-lesions-shearlet-transform-and-local-binary-pattern-histogram-techniques
#8
U Rajendra Acharya, Wei Lin Ng, Kartini Rahmat, Vidya K Sudarshan, Joel E W Koh, Jen Hong Tan, Yuki Hagiwara, Arkadiusz Gertych, Farhana Fadzli, Chai Hong Yeong, Kwan Hoong Ng
Shear wave elastography (SWE) examination using ultrasound elastography (USE) is a popular imaging procedure for obtaining elasticity information of breast lesions. Elasticity parameters obtained through SWE can be used as biomarkers that can distinguish malignant breast lesions from benign ones. Furthermore, the elasticity parameters extracted from SWE can speed up the diagnosis and possibly reduce human errors. In this paper, Shearlet transform and local binary pattern histograms (LBPH) are proposed as an original algorithm to differentiate malignant and benign breast lesions...
October 2, 2017: Computers in Biology and Medicine
https://www.readbyqxmd.com/read/29031098/stepwise-construction-of-a-metabolic-network-in-event-b-the-heat-shock-response
#9
Usman Sanwal, Luigia Petre, Ion Petre
There is a high interest in constructing large, detailed computational models for biological processes. This is often done by putting together existing submodels and adding to them extra details/knowledge. The result of such approaches is usually a model that can only answer questions on a very specific level of detail, and thus, ultimately, is of limited use. We focus instead on an approach to systematically add details to a model, with formal verification of its consistency at each step. In this way, one obtains a set of reusable models, at different levels of abstraction, to be used for different purposes depending on the question to address...
September 30, 2017: Computers in Biology and Medicine
https://www.readbyqxmd.com/read/29031083/neural-network-robust-tracking-control-with-adaptive-critic-framework-for-uncertain-nonlinear-systems
#10
Ding Wang, Derong Liu, Yun Zhang, Hongyi Li
In this paper, we aim to tackle the neural robust tracking control problem for a class of nonlinear systems using the adaptive critic technique. The main contribution is that a neural-network-based robust tracking control scheme is established for nonlinear systems involving matched uncertainties. The augmented system considering the tracking error and the reference trajectory is formulated and then addressed under adaptive critic optimal control formulation, where the initial stabilizing controller is not needed...
September 21, 2017: Neural Networks: the Official Journal of the International Neural Network Society
https://www.readbyqxmd.com/read/29031049/heart-valve-scaffold-fabrication-bioinspired-control-of-macro-scale-morphology-mechanics-and-micro-structure
#11
Antonio D'Amore, Samuel K Luketich, Giuseppe M Raffa, Salim Olia, Giorgio Menallo, Antonino Mazzola, Flavio D'Accardi, Tamir Grunberg, Xinzhu Gu, Michele Pilato, Marina V Kameneva, Vinay Badhwar, William R Wagner
Valvular heart disease is currently treated with mechanical valves, which benefit from longevity, but are burdened by chronic anticoagulation therapy, or with bioprosthetic valves, which have reduced thromboembolic risk, but limited durability. Tissue engineered heart valves have been proposed to resolve these issues by implanting a scaffold that is replaced by endogenous growth, leaving autologous, functional leaflets that would putatively eliminate the need for anticoagulation and avoid calcification. Despite the diversity in fabrication strategies and encouraging results in large animal models, control over engineered valve structure-function remains at best partial...
October 6, 2017: Biomaterials
https://www.readbyqxmd.com/read/29031039/the-anti-apoptotic-mechanism-of-metformin-against-apoptosis-induced-by-ionizing-radiation-in-human-peripheral-blood-mononuclear-cells
#12
S Kolivand, E Motevaseli, M Cheki, A Mahmoudzadeh, A Shirazi, V Fait
BACKGROUND: In a previous article, we showed that metformin (MET) can reduce ionizing radiation (IR) induced apoptosis in human peripheral blood mononuclear cells. However, the anti-apoptotic mechanism of MET against IR remains unclear. The present study attempts to investigate the mechanism of action of MET in limiting X-ray induced apoptosis in human peripheral blood mononuclear cells. MATERIAL AND METHODS: Mononuclear cells were treated with MET for 2 hours and irradiated with 6 MV X-rays...
2017: Klinická Onkologie: Casopis Ceské a Slovenské Onkologické Spolecnosti
https://www.readbyqxmd.com/read/29031022/fetal-analysis-with-invasive-method-fa-i-and-fetal-analysis-with-non-invasive-method-fa-ni-replacing-current-deceptively-imprecise-clinical-nomenclature
#13
Frank A Chervenak, Laurence B McCullough, Joachim Dudenhausen
There is a problem with the current nomenclature of prenatal evaluation. The current nomenclature of "prenatal testing" and "prenatal screening" - along with their subsets of "ultrasound testing," "ultrasound screening," "non-invasive prenatal testing," "non-invasive prenatal screening," and "prenatal diagnosis" - has become so imprecise that clinical misinterpretation and distortion of the informed consent process are increasingly difficult to avoid. To remedy this problem, we propose a new, precise nomenclature: "fetal analysis with invasive method" (FA-I) and "fetal analysis with non-invasive method," (FA-NI) using various techniques...
October 14, 2017: Journal of Perinatal Medicine
https://www.readbyqxmd.com/read/29029360/laser-desorption-ionization-mass-spectrometry-recent-progress-in-matrix-free-and-label-assisted-techniques
#14
REVIEW
Arundhoti Mandal, Monisha Singha, Partha Sarathi Addy, Amit Basak
The MALDI-based mass spectrometry, over the last three decades, has become an important analytical tool. It is a gentle ionization technique, usually applicable to detect and characterize analytes with high molecular weights like proteins and other macromolecules. The earlier difficulty of detection of analytes with low molecular weights like small organic molecules and metal ion complexes with this technique arose due to the cluster of peaks in the low molecular weight region generated from the matrix. To detect such molecules and metal ion complexes, a four-prong strategy has been developed...
October 13, 2017: Mass Spectrometry Reviews
https://www.readbyqxmd.com/read/29029355/over-sewing-of-staple-line-in-laparoscopic-sleeve-gastrectomy-initial-experience-of-a-comparative-study
#15
Giorgio Lisi, Roberto Rossini, Irene Gentile, Giacomo Ruffo
INTRODUCTION: The main drawback of laparoscopic sleeve gastrectomy (LSG) is the severity of postoperative complications. Staple line reinforcement (SLR) is strongly advocated. Recently, over-sewing has been proposed as a cost-effective and helpful method for reinforcing the staple line. The purpose of this study was to report our initial experience with LSG comparing over-sewing over the entire staple line with the upper-third staple line. MATERIALS AND METHODS: All obese patients seen at Negrar Sacro Cuore, Don Calabria Hospital were entered into our prospective database and were retrospectively evaluated...
October 12, 2017: Surgical Technology International
https://www.readbyqxmd.com/read/29029335/cortical-reorganization-in-dual-innervation-by-single-peripheral-nerve
#16
Mou-Xiong Zheng, Yun-Dong Shen, Xu-Yun Hua, Ao-Lin Hou, Yi Zhu, Wen-Dong Xu
BACKGROUND: Functional recovery after peripheral nerve injury and repair is related with cortical reorganization. However, the mechanism of innervating dual targets by 1 donor nerve is largely unknown. OBJECTIVE: To investigate the cortical reorganization when the phrenic nerve simultaneously innervates the diaphragm and biceps. METHODS: Total brachial plexus (C5-T1) injury rats were repaired by phrenic nerve-musculocutaneous nerve transfer with end-to-side (n = 15) or end-to-end (n = 15) neurorrhaphy...
September 21, 2017: Neurosurgery
https://www.readbyqxmd.com/read/29029321/rapid-novel-facile-biosynthesized-silver-nanoparticles-from-bacterial-release-induce-biogenicity-and-concentration-dependent-in-vivo-cytotoxicity-with-embryonic-zebrafish-a-mechanistic-insight
#17
Suresh K Verma, Ealisha Jha, Pritam Kumar Panda, Anurag Mishra, Arun Thirumurugan, Biswadeep Das, S K S Parashar, Mrutyunjay Suar
In this study, rapid one step facile synthesis of silver nanoparticles (AgNP) was done using culture supernatant of two Gram positive (B.thuringiensis, S.aureus) and Gram negative (E.coli, S.typhimurium) bacterial strains and were termed as "BTAgNP", "SAAgNP", "ECAgNP" and "STAgNP" respectively. Synthesized AgNPs were well characterized with the help of different standard techniques like FESEM, DLS, UV-Vis spectroscopy and FTIR. Mechanism of AgNPs synthesis was elucidated using in silico approach. In vivo cytotoxicity of synthesized AgNPs was assessed in embryonic Zebrafish model with the help of uptake, oxidative stress and apoptosis induction experimental assays and the mechanism was investigated through in silico approach at the molecular level...
September 28, 2017: Toxicological Sciences: An Official Journal of the Society of Toxicology
https://www.readbyqxmd.com/read/29029292/minimizing-retraction-by-pia-arachnoidal-10-0-sutures-in-intrasulcal-dissection
#18
Kutluay Uluc, Ulas Cikla, Deniz B Morkan, Alperen Sirin, Azam S Ahmed, Kyle Swanson, Mustafa K Baskaya
BACKGROUND: In contemporary microneurosurgery reducing retraction-induced injury to the brain is essential. Self-retaining retractor systems are commonly used to improve visualization and decrease the repetitive microtrauma, but sometimes self-retaining retractor systems can be cumbersome and the force applied can cause focal ischemia or contusions. This may increase the morbidity and mortality. Here, we describe a technique of retraction using 10-0 sutures in the arachnoid. OBJECTIVE: To evaluate the imaging and clinical results in patients where 10-0 suture retraction was used to aid the surgical procedure...
September 19, 2017: Operative Neurosurgery (Hagerstown, Md.)
https://www.readbyqxmd.com/read/29029290/nmda-receptor-mediated-ca2-influx-in-the-absence-of-mg2-block-disrupts-rest-activity-rhythms-in-drosophila
#19
Qian Song, Ge Feng, Jiaxing Zhang, Xuechun Xia, Min Ji, Lei Lv, Yong Ping
Introduction: The correlated activation of pre- and postsynaptic neurons is essential for the NMDA receptor-mediated Ca2+ influx by removing Mg2+ from block site and NMDA receptors have been implicated in phase resetting of circadian clocks. So we assessed rest:activity rhythms in Mg2+ block defective animals. Methods: Using Drosophila locomotor monitoring system, we checked circadian rest:activity rhythms of different mutants under constant darkness (DD) and light:dark (LD) conditions...
October 3, 2017: Sleep
https://www.readbyqxmd.com/read/29029275/surgical-management-of-intrinsic-tumors-of-the-facial-nerve
#20
Sampath Chandra Prasad, Melissa Laus, Manjunath Dandinarasaiah, Enrico Piccirillo, Alessandra Russo, Abdelkader Taibah, Mario Sanna
BACKGROUND: Intrinsic tumors of the facial nerve are a rare entity. Dealing with this subset of tumors is challenging both in terms of decision making and surgical intervention. OBJECTIVE: To review the outcomes of surgical management of facial nerve tumors and cable nerve graft interpositioning. METHODS: A retrospective analysis was performed at a referral center for skull base pathology. One hundred fifteen patients who were surgically treated for facial nerve tumors were included...
September 29, 2017: Neurosurgery
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