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https://www.readbyqxmd.com/read/29909597/recent-advances-and-future-of-gene-therapy-for-bone-regeneration
#1
REVIEW
Galina Shapiro, Raphael Lieber, Dan Gazit, Gadi Pelled
PURPOSE OF REVIEW: The purpose of this review is to discuss the recent advances in gene therapy as a treatment for bone regeneration. While most fractures heal spontaneously, patients who present with fracture nonunion suffer from prolonged pain, disability, and often require additional operations to regain musculoskeletal function. RECENT FINDINGS: In the last few years, BMP gene delivery by means of electroporation and sonoporation resulted in repair of nonunion bone defects in mice, rats, and minipigs...
June 16, 2018: Current Osteoporosis Reports
https://www.readbyqxmd.com/read/29909577/population-pharmacokinetic-model-for-tumescent-lidocaine-in-women-undergoing-breast-cancer-surgery
#2
Camille Riff, Aurélie Bourgoin, Amelie Marsot, Laurent Allanioux, Marc Leone, Olivier Blin, Romain Guilhaumou
PURPOSE: Tumescent lidocaine anesthesia (TLA) is an opportunity to perform mastectomy for breast cancer without general anesthesia in elderly women. Few reports are available on the pharmacokinetics of lidocaine in a context of TLA during a unilateral mastectomy. The aim of this study was to describe lidocaine pharmacokinetics in elderly women undergoing breast cancer surgery after TLA and to explore the risk of the toxicity of this technique. METHODS: A prospective study was conducted to examine the pharmacokinetics of lidocaine in women undergoing TLA...
June 16, 2018: European Journal of Clinical Pharmacology
https://www.readbyqxmd.com/read/29909567/paravertebral-blocks-reduce-the-risk-of-postoperative-urinary-retention-in-inguinal-hernia-repair
#3
E Bojaxhi, J Lee, S Bowers, R D Frank, S H Pak, A Rosales, S Padron, R A Greengrass
PURPOSE: Inguinal hernia repair and general anesthesia (GA) are known risk factors for urinary retention. Paravertebral blocks (PVBs) have been utilized to facilitate enhanced recovery after surgery. We evaluate the benefit of incorporating PVBs into our anesthetic technique in a large cohort of ambulatory patients undergoing inguinal hernia repair. METHODS: Records of 619 adults scheduled for ambulatory inguinal hernia repair between 2010 and 2015 were reviewed and categorized based on anesthetic and surgical approach [GA and open (GAO), GA and laparoscopic (GAL), PVB and open (PVBO), and GA/PVB and open (GA/PVBO)]...
June 16, 2018: Hernia: the Journal of Hernias and Abdominal Wall Surgery
https://www.readbyqxmd.com/read/29909556/the-urinary-microbiome-in-women-with-mixed-urinary-incontinence-compared-to-similarly-aged-controls
#4
Yuko M Komesu, Holly E Richter, Benjamin Carper, Darrell L Dinwiddie, Emily S Lukacz, Nazema Y Siddiqui, Vivian W Sung, Halina M Zyczynski, Beri Ridgeway, Rebecca G Rogers, Lily A Arya, Donna Mazloomdoost, Marie G Gantz
INTRODUCTION & HYPOTHESIS: Previous studies have suggested that women with urinary incontinence have an altered urinary microbiome. We hypothesized that the microbiome in women with mixed urinary incontinence (MUI) differed from controls and tested this hypothesis using bacterial gene sequencing techniques. METHODS: This multicenter study compared the urinary microbiome in women with MUI and similarly aged controls. Catheterized urine samples were obtained; v4-6 regions of the 16S rRNA gene were sequenced to identify bacteria...
June 16, 2018: International Urogynecology Journal
https://www.readbyqxmd.com/read/29909552/early-outcomes-of-270-degree-spinal-canal-decompression-by-using-tessys-isee-technique-in-patients-with-lumbar-spinal-stenosis-combined-with-disk-herniation
#5
Chengjie Xiong, Tao Li, Hui Kang, Hao Hu, Jing Han, Feng Xu
PURPOSE: Traditional open approach is an efficient way to treat lumbar spinal stenosis (SS) combined with disk herniation (DH); however, risk factors such as advanced age, osteoporosis etc. are associated with the complications after the surgery. This study aims to analyze the early clinical and radiological outcomes of treatment on SS&DH by using newly developed minimal invasive TESSYS-ISEE technique. METHODS: Patients with limp and unilateral lower limb radiculopathy underwent minimal invasive surgery by using TESSYS-ISEE technique...
June 16, 2018: European Spine Journal
https://www.readbyqxmd.com/read/29909528/accuracy-of-computer-aided-design-models-of-the-jaws-produced-using-ultra-low-mdct-doses-and-asir-and-mbir
#6
Asma'a A Al-Ekrish, Sara A Alfadda, Wadea Ameen, Romed Hörmann, Wolfgang Puelacher, Gerlig Widmann
PURPOSE: To compare the surface of computer-aided design (CAD) models of the maxilla produced using ultra-low MDCT doses combined with filtered backprojection (FBP), adaptive statistical iterative reconstruction (ASIR) and model-based iterative reconstruction (MBIR) reconstruction techniques with that produced from a standard dose/FBP protocol. METHODS: A cadaveric completely edentulous maxilla was imaged using a standard dose protocol (CTDIvol: 29.4 mGy) and FBP, in addition to 5 low dose test protocols (LD1-5) (CTDIvol: 4...
June 16, 2018: International Journal of Computer Assisted Radiology and Surgery
https://www.readbyqxmd.com/read/29909512/the-effectiveness-of-endoscopic-gastroplasty-for-obesity-treatment-according-to-fda-thresholds-systematic-review-and-meta-analysis-based-on-randomized-controlled-trials
#7
REVIEW
Antonio Coutinho Madruga-Neto, Wanderley Marques Bernardo, Diogo Turiani Hourneaux de Moura, Vitor Ottoboni Brunaldi, Rafael Krieger Martins, Iatagan Rocha Josino, Eduardo Turiani Hourneaux de Moura, Thiago Ferreira de Souza, Marco Aurélio Santo, Eduardo Guimarães Hourneaux de Moura
Endoscopic bariatric therapies (EBTs) are promising alternatives to conventional surgery for obesity. The aim of this study is to compare efficacy and safety through a systematic review and meta-analysis of the endoscopic gastroplasty techniques versus conservative treatment. We searched MEDLINE, EMBASE, Cochrane CENTRAL, Lilacs/Bireme. Randomized controlled trials (RCTs) enrolling obese patients comparing endoscopic gastroplasty to sham or diet/exercise were considered eligible. Among 6014 records, three RCTs were selected for meta-analysis...
June 16, 2018: Obesity Surgery
https://www.readbyqxmd.com/read/29909465/anteromedial-femoral-neck-plate-with-cannulated-screws-for-the-treatment-of-irreducible-displaced-femoral-neck-fracture-in-young-patients-a-preliminary-study
#8
Linbo Zhuang, Lisheng Wang, Dongming Xu, Zhiyong Wang
BACKGROUNDS: Although most displaced femoral neck fractures of young patients can be repositioned successfully by closed reduction, there are still some can not repositioned successfully by this way and open reductions are required. This type of fracture was defined as irreducible displaced femoral neck fracture in this study. The purpose of this study was to introduce a new technique using anteromedial femoral neck plate with cannulated screws fixation by open reduction for the treatment of irreducible displaced femoral neck fractures in young patients...
June 16, 2018: European Journal of Trauma and Emergency Surgery: Official Publication of the European Trauma Society
https://www.readbyqxmd.com/read/29909459/electrochemical-nonenzymatic-sensor-for-cholesterol-determination-in-food
#9
Ksenia Derina, Elena Korotkova, Yekaterina Taishibekova, Lyazat Salkeeva, Bohumil Kratochvil, Jiri Barek
The treatment of some inborn metabolism errors requires cholesterol substitution therapy. Cholesterol plays a vital role in the human body. Therefore, the majority of cholesterol determination techniques are targeted to blood and blood serum. Nevertheless, cholesterol determination in food is important as well. In this paper, cholesterol determination using differential pulse voltammetry (DPV) in dairy products (e.g., milk, clotted cream, yogurt, butter, etc.) is reported with a novel nonenzymatic sensor based on diphosphonic acid of 1,4-diacetylglycoluril (DPADGU) as an electrode surface modifier...
June 16, 2018: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/29909455/comparison-of-%C3%AE-atr-ftir-spectroscopy-and-py-gcms-as-identification-tools-for-microplastic-particles-and-fibers-isolated-from-river-sediments
#10
Andrea Käppler, Marten Fischer, Barbara M Scholz-Böttcher, Sonja Oberbeckmann, Matthias Labrenz, Dieter Fischer, Klaus-Jochen Eichhorn, Brigitte Voit
In recent years, many studies on the analysis of microplastics (MP) in environmental samples have been published. These studies are hardly comparable due to different sampling, sample preparation, as well as identification and quantification techniques. Here, MP identification is one of the crucial pitfalls. Visual identification approaches using morphological criteria alone often lead to significant errors, being especially true for MP fibers. Reliable, chemical structure-based identification methods are indispensable...
June 16, 2018: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/29909446/graft-considerations-in-posterior-cruciate-ligament-reconstruction
#11
REVIEW
Pierce Johnson, Sean M Mitchell, Simon Görtz
PURPOSE OF REVIEW: To highlight current and established concepts regarding PCL injury and reconstruction. RECENT FINDINGS: Recent biomechanical and clinical studies have brought attention to improved surgical techniques and clinical outcomes of PCL reconstruction. In contrast to anterior cruciate ligament (ACL) injuries, isolated posterior cruciate ligament (PCL) injuries occur much less frequently and have traditionally been treated non-operatively. Even when a PCL injury meets operative indications, outcomes of PCL reconstruction historically do not match the success rates of ACL reconstruction procedures...
June 16, 2018: Current Reviews in Musculoskeletal Medicine
https://www.readbyqxmd.com/read/29909434/ionomer-and-protein-size-analysis-by-analytical-ultracentrifugation-and-electrospray-scanning-mobility-particle-sizer
#12
Simon E Wawra, Martin Thoma, Johannes Walter, Christian Lübbert, Thaseem Thajudeen, Cornelia Damm, Wolfgang Peukert
By combining analytical ultracentrifugation (AUC) in liquid phase and scanning mobility particle sizer (SMPS) in the gas phase, additional information on the particle size and morphology has been obtained for rigid particles. In this paper, we transfer this concept to soft particles, allowing us to analyze the size and molar mass of the short side chain perfluorosulfonic acid ionomer Aquivion® in a dilute aqueous suspension. The determination of the primary size and exact molar mass of this class of polymers is challenging since they are optically transparent and due to the formation of different aggregate structures depending on the concentration and solvent properties...
June 16, 2018: European Biophysics Journal: EBJ
https://www.readbyqxmd.com/read/29909430/an-intra-oral-approach-to-facial-skin-lumps-a-move-towards-scarless-surgery
#13
Arpan Tahim, Sarah Ali, Leo Cheng
INTRODUCTION: The aim of surgical management of benign facial skin lumps is to remove the lesion in its entirety, with minimal damage to adjacent tissue structures, while maintaining facial aesthetics. A method to achieve these goals is to remove peri-oral lesions in the labial and buccal zones via an intra-oral approach. We report an extended cases series of facial skin lumps that were excised using an intra-oral approach. METHODS: A retrospective analysis was performed on all patients who underwent excisions of benign cutaneous lesions on the face via an intra-oral approach...
June 16, 2018: Oral and Maxillofacial Surgery
https://www.readbyqxmd.com/read/29909421/application-of-isobaric-tags-for-relative-and-absolute-quantification-itraq-coupled-with-two-dimensional-liquid-chromatography-tandem-mass-spectrometry-in-quantitative-proteomic-analysis-for-discovery-of-serum-biomarkers-for-idiopathic-pulmonary-fibrosis
#14
Ying Zhang, Qian Xin, Zhen Wu, Chaochao Wang, Yongbin Wang, Qian Wu, Rui Niu
BACKGROUND The present study was performed to explore the presence of informative protein biomarkers of human serum proteome in idiopathic pulmonary fibrosis (IPF). MATERIAL AND METHODS Serum samples were profiled using iTRAQ coupled with two-dimensional liquid chromatography/tandem mass spectrometry (2D-LC-MS/MS) technique, and ELISA was used to validate candidate biomarkers. RESULTS A total of 394 proteins were identified and 97 proteins were associated with IPF. Four biomarker candidates generated from iTRAQ experiments - CRP, fibrinogen-α chain, haptoglobin, and kininogen-1 - were successfully verified using ELISA...
June 17, 2018: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/29909418/cell-block-in-cytological-diagnostics-review-of-preparatory-techniques
#15
REVIEW
Leena Krogerus, Ivana Kholová
OBJECTIVE: The cell block (CB) technique refers to the processing of sediments, blood clots, or grossly visible tissue fragments from cytological specimens into paraffin blocks that can be cut and stained by the same methods used for histopathology. The technique brings additional tissue architectural information. CB can be used for ancillary techniques such as immunocytochemistry and molecular techniques. STUDY DESIGN: We reviewed the literature on the various preparatory techniques of CBs...
June 15, 2018: Acta Cytologica
https://www.readbyqxmd.com/read/29909384/common-femoral-artery-aneurysm-repair-using-bifurcated-graft
#16
Sesi Ayodele Hotonu, Charles Henry North Johnson, Nisheeth Kansal, Vish Bhattacharya
Common femoral artery aneurysms are rare, and surgical repair is indicated if they are significantly large, or if they are symptomatic (thrombosis causing limb ischaemia and compression of surrounding structures). Synthetic grafts are preferred, especially in cases involving large aneurysms, or the bifurcation of the common femoral artery. We present a case of bilateral common femoral artery aneurysms extending into the bifurcation repaired using a synthetic graft which is traditionally used for an axillobifemoral bypass...
June 15, 2018: BMJ Case Reports
https://www.readbyqxmd.com/read/29909379/unique-percutaneous-direct-puncture-technique-for-occlusion-of-a-hypoglossal-canal-dural-arteriovenous-fistula
#17
Orlando M Diaz, Maria M Toledo, John O F Roehm, Richard P Klucznik, Ponraj Chinnadurai, Gloria Viviana Lopez, Gavin W Britz
PURPOSE: To report percutaneous transcranial puncture, embolisation and occlusion of a very symptomatic hypoglossal canal/anterior condylar vein dural arteriovenous fistula (DAVF) using syngo iGuide navigational software in a patient in whom transarterial and transvenous embolisation and surgery had failed. METHODS: After unsuccessful arterial and venous embolisation and surgical treatment of a symptomatic hypoglossal canal DAVF, a 47-year-old man was transferred for further management...
June 16, 2018: Journal of Neurointerventional Surgery
https://www.readbyqxmd.com/read/29909363/excitability-tests-using-high-density-surface-emg-a-novel-approach-to-studying-single-motor-units
#18
Boudewijn T H M Sleutjes, Judith Drenthen, Ernest Boskovic, Leonard J van Schelven, Maria O Kovalchuk, Paul G E Lumens, Leonard H van den Berg, Hessel Franssen
OBJECTIVE: To study excitability of single motor units (MUs) using high-density surface-EMG. METHODS: Motor unit action potentials (MUAPs) were evoked by submaximal stimulation of the median nerve at the wrist and recorded with a 9 × 14 electrode grid on the skin overlying the thenar muscles. For excitability tests of single MUs, the most optimal specific single-channel surface-EMG signal was selected based on the spatiotemporal profile of single MUs. RESULTS: Axonal excitability measures were successfully obtained from 14 single MUs derived from ten healthy subjects...
June 1, 2018: Clinical Neurophysiology: Official Journal of the International Federation of Clinical Neurophysiology
https://www.readbyqxmd.com/read/29909351/a-contribution-to-the-characterization-of-the-silicate-water-interface-part-i-implication-of-a-new-polished-sample-hydration-technique
#19
T Sowoidnich, L Gordon, C Naber, F Bellmann, J Neubauer, D Joester
The analysis of the atomic composition of the interface between tricalcium silicate (C3 S), the main compound of Ordinary Portland Cement, and surrounding solution is still a challenging task. At the same time, that knowledge is of profound importance for describing the basic processes during hydration. By means of Scanning Electron Microscopy (SEM) and Atom Probe Tomography (APT) we combine modern techniques in order to shed light on this topic in the present study. The results of these methods are compared with conduction calorimetry as a standard technique to study the hydration kinetics of cement...
June 11, 2018: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/29909331/particle-scale-understanding-of-cypermethrin-in-sediment-desorption-bioavailability-and-bioaccumulation-in-benthic-invertebrate-lumbriculus-variegatus
#20
Jie Zhang, Jing You, Huizhen Li, W Tyler Mehler, Eddy Y Zeng
Influence of sediment particle size on the desorption, bioavailability, and bioaccumulation potential of cypermethrin was investigated in the present study using two biomimetic techniques (Tenax extraction and solid-phase microextraction (SPME)) and bioaccumulation testing with Lumbriculus variegatus. A field-collected sediment was wet sieved to obtain five particle-size fractions (<20, 20-63, 63-180, 180-500, and >500 μm) and used for cypermethrin spiking. The finest sediment (<20 μm) had the highest rapid desorption fraction (Fr ) and rate (kr ) when compared to coarser sediments...
June 14, 2018: Science of the Total Environment
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