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https://www.readbyqxmd.com/read/29236340/flow-cytometry-a-versatile-technology-for-specific-quantification-and-viability-assessment-of-microorganisms-in-multi-strain-probiotic-products
#1
Camille Chiron, Thomas A Tompkins, Pierre Burguière
AIMS: Classical microbiology techniques are the gold standard for probiotic enumeration. However, these techniques are limited by parameters of time, specificity and incapacity to detect viable but non-culturable (VBNC) microorganisms and non-viable cells. The aim of the study was to evaluate flow cytometry as a novel method for the specific quantification of viable and non-viable probiotics in multi-strain products. METHODS AND RESULTS: Custom polyclonal antibodies were produced against five probiotic strains from different species (Bifidobacterium bifidum R0071, Bifidobacterium longum ssp...
December 13, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/29236261/reactivation-assay-to-identify-direct-targets-of-the-nonsense-mediated-mrna-decay-pathway-in-drosophila
#2
Jonathan O Nelson, Mark M Metzstein
Transcriptome analysis provides a snapshot of cellular gene expression and is used to determine how cells and organisms respond to genetic or environmental changes. Identifying the transcripts whose expression levels are regulated directly by the manipulation being examined from those whose expression changes as a secondary cause from the primary changes requires additional analyses. Here we present a technique used to distinguish direct targets of the nonsense-mediated mRNA decay (NMD) pathway in Drosophila from secondary gene expression effects caused by loss of this pathway...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29236257/identifying-risc-components-using-ago2-immunoprecipitation-and-mass-spectrometry
#3
Tingfang Yi
Complex immunoprecipitation (Co-IP) is a powerful technique for precipitating an intact protein complex out of solution and cell lysates using an antibody that specifically binds to a particular protein in a large complex of proteins. Mass spectrometry (MS) is used to identify, sequence, and quantify proteins. RNA-induced silencing complexes (RISCs), Ago2 centered protein assemblies, are essential for miRNA mediated RNA decay and gene expression regulation; however, the complete list of RISCs is unknown. Here we describe methods used to combine IP and MS to identify new components of RISCs...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29236255/identifying-mirna-targets-using-ago-ripseq
#4
Rebecca Petri, Johan Jakobsson
microRNAs (miRNA) are small, noncoding RNAs that bind to messenger RNAs (mRNAs) and regulate their activity. They are, therefore, important posttranscriptional regulators. In recent years it has become clear that miRNAs regulate large genetic networks, rather than single genes, and that one gene can be targeted by several miRNAs. To understand the role of miRNAs in cells or tissues, it is therefore important to analyze the targetome of miRNAs. Here, we present a technique called Argonaute-RNA Immunoprecipitation (AGO-RIP) which takes advantages of the fact that miRNAs and their targets are directly bound by the Argonaute protein family...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29236253/iclip-of-the-piwi-protein-aubergine-in-drosophila-embryos
#5
Bridlin Barckmann, Jérémy Dufourt, Martine Simonelig
Piwi-interacting RNAs (piRNAs) are a class of small noncoding RNAs bound to specific Argonaute proteins, the PIWI proteins. piRNAs target mRNAs by complementarity to silence them; they play an important role in the repression of transposable elements in the germ line of many species. piRNAs and PIWI proteins are also involved in diverse biological processes through their role in the regulation of cellular mRNAs. In the Drosophila embryo, they contribute to the maternal mRNA decay occurring during the maternal-to-zygotic transition...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29236247/5-bromouridine-ip-chase-bric-seq-to-determine-rna-half-lives
#6
Toshimichi Yamada, Naoto Imamachi, Rena Onoguchi-Mizutani, Katsutoshi Imamura, Yutaka Suzuki, Nobuyoshi Akimitsu
Analysis of RNA stability at genome-wide level is an advanced method in RNA biology that examines the half-life of each transcript. In particular, a pulse-labeling method using uridine analogs enables the determination of half-life of each transcript under physiologically undisturbed conditions. The technique involves pulse labeling of endogenous RNAs in mammalian cells with 5'-bromouridine (BrU), followed by measuring the chronological decrease of BrU-labeled RNAs using deep sequencing (BRIC-seq). Here, we describe a detailed protocol and technical tips for BRIC-seq...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29236223/transit-and-lifespan-in-neutrophil-production-implications-for-drug-intervention
#7
Daniel Câmara De Souza, Morgan Craig, Tyler Cassidy, Jun Li, Fahima Nekka, Jacques Bélair, Antony R Humphries
A comparison of the transit compartment ordinary differential equation modelling approach to distributed and discrete delay differential equation models is studied by focusing on Quartino's extension to the Friberg transit compartment model of myelosuppression, widely relied upon in the pharmaceutical sciences to predict the neutrophil response after chemotherapy, and on a QSP delay differential equation model of granulopoiesis. An extension to the Quartino model is provided by considering a general number of transit compartments and introducing an extra parameter that allows for the decoupling of the maturation time from the production rate of cells...
December 13, 2017: Journal of Pharmacokinetics and Pharmacodynamics
https://www.readbyqxmd.com/read/29236212/post-operative-refractive-prediction-error-after-phacovitrectomy-a-retrospective-study
#8
Gijsbert J Hötte, Daniël P de Bruyn, Joeri de Hoog
INTRODUCTION: Many authors have reported on a myopic post-operative refractive prediction error when combining phacoemulsification with pars plana vitrectomy (phacovitrectomy). In this study we evaluate the amount of this error in our facility and try to elucidate the various factors involved. METHODS: This was a retrospective study which included 140 patients who underwent phacovitrectomy (39 with macular holes, 88 with puckers, and 13 with floaters). Post-operative refractive error was defined as the difference between the actual spherical equivalent (SEQ) and expected SEQ based on the SRK/T and Holladay-II formulas...
December 13, 2017: Ophthalmology and Therapy
https://www.readbyqxmd.com/read/29236177/effect-of-er-yag-laser-enamel-conditioning-and-moisture-on-the-microleakage-of-a-hydrophilic-sealant
#9
Zeynep Aslı Güçlü, Andrew Paul Hurt, Nazmiye Dönmez, Nichola Jayne Coleman
For a given sealant, successful pit and fissure sealing is principally governed by the enamel conditioning technique and the presence of moisture contamination. A new generation of hydrophilic resin sealants is reported to tolerate moisture. This study investigates the impact of Er:YAG laser pre-conditioning and moisture contamination on the microleakage of a recent hydrophilic sealant. Occlusal surfaces of extracted human molars were either acid etched (n = 30), or successively lased and acid etched (n = 30)...
December 13, 2017: Odontology
https://www.readbyqxmd.com/read/29236175/an-assessment-of-metal-humus-complexes-in-river-waters-of-the-upper-amur-basin-russia
#10
Svetlana Levshina
The dissolved metal concentrations have been assessed for Co, Cr, Cu, Fe, Mn, Ni, V, Zn, Ca, Mg, and Cd, and their complex compounds with humic substances (Me-HS complexes) in river waters of the Upper Amur basin using the inductively coupled plasma-mass spectrometry technique. The study shows an increase in the amount of Me-HS complexes from the southwestern steppe and forest-steppe to the northeastern mountainous taiga areas. In alkali waters of the steppe and forest-steppe areas with low concentrations of HS, the amount of Zn-HS complexes increases considerably...
December 13, 2017: Environmental Monitoring and Assessment
https://www.readbyqxmd.com/read/29236134/determination-of-halogenated-flame-retardants-by-gc-api-ms-ms-and-gc-ei-ms-a-multi-compound-multi-matrix-method
#11
Frank Neugebauer, Annekatrin Dreyer, Nina Lohmann, Jan Koschorreck
The extensive application of halogenated flame retardants has led to their widespread distribution in the environment. Recently, concerns emerged regarding their potential persistence, (bio)accumulation, and/or toxicity. Particularly halogenated flame retardants based on norbornene structures, like Dechlorane Plus as well as other brominated PBDE replacements, generically called emerging, novel, or alternative flame retardants, are in the focus of interest. A comprehensive analytical method for the determination of 21 halogenated flame retardants (HFRs) of different substance classes (dechloranes, brominated aromates, brominated ethers, cyclic BFR) in a broad variety of matrices (tree leaves, fish fillet, birds eggs, suspended particles) was developed in order to assess their environmental levels as well as temporal trends, especially for the use within environmental specimen banks (ESBs)...
December 13, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/29236112/interfacial-and-thermal-energy-driven-growth-and-evolution-of-langmuir-schaefer-monolayers-of-au-nanoparticles
#12
Mala Mukhopadhyay, S Hazra
Structures of Langmuir-Schaefer (LS) monolayers of thiol-coated Au-nanoparticles (DT-AuNPs) deposited on H-terminated and OTS self-assembled Si substrates (of different hydrophobic strength and stability) and their evolution with time under ambient conditions, which plays an important role for their practical use as 2D-nanostructures over large areas, were investigated using the X-ray reflectivity technique. The strong effect of substrate surface energy (γ) on the initial structures and the competitive role of room temperature thermal energy (kT) and the change in interfacial energy (Δγ) at ambient conditions on the evolution and final structures of the DT-AuNP LS monolayers are evident...
December 13, 2017: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/29236078/an-artificial-neural-network-for-movement-pattern-analysis-to-estimate-blood-alcohol-content-level
#13
Pedram Gharani, Brian Suffoletto, Tammy Chung, Hassan A Karimi
Impairments in gait occur after alcohol consumption, and, if detected in real-time, could guide the delivery of "just-in-time" injury prevention interventions. We aimed to identify the salient features of gait that could be used for estimating blood alcohol content (BAC) level in a typical drinking environment. We recruited 10 young adults with a history of heavy drinking to test our research app. During four consecutive Fridays and Saturdays, every hour from 8 p.m. to 12 a.m., they were prompted to use the app to report alcohol consumption and complete a 5-step straight-line walking task, during which 3-axis acceleration and angular velocity data was sampled at a frequency of 100 Hz...
December 13, 2017: Sensors
https://www.readbyqxmd.com/read/29236074/morphological-and-optical-characteristics-of-chitosan-1-x-cuox-4-%C3%A2-x-%C3%A2-12-based-polymer-nano-composites-optical-dielectric-loss-as-an-alternative-method-for-tauc-s-model
#14
Shujahadeen B Aziz
In this work, copper (Cu) nanoparticles with observable surface plasmonic resonance (SPR) peaks were synthesized by an in-situ method. Chitosan host polymer was used as a reduction medium and a capping agent for the Cu nanoparticles. The surface morphology of the samples was investigated through the use of scanning electron micrograph (SEM) technique. Copper nanoparticles appeared as chains and white specks in the SEM images. The strong peaks due to the Cu element observed in the spectrum of energy dispersive analysis of X-rays...
December 13, 2017: Nanomaterials
https://www.readbyqxmd.com/read/29236060/the-use-of-poly-d-l-lactic-acid-pdlla-devices-for-bone-augmentation-techniques-a-systematic-review
#15
REVIEW
Marco Annunziata, Livia Nastri, Gennaro Cecoro, Luigi Guida
Poly-d,l-lactic acid (PDLLA) has been proposed in dentistry for regenerative procedures in the form of membranes, screws, and pins. The aim of this review was to evaluate the efficacy of bone augmentation techniques using PDLLA devices. A literature search was carried out by two independent and calibrated reviewers. All interventional and observational studies assessing the efficacy of bone augmentation techniques using PDLLA devices were included. Six studies were included. The relevant variability of design and methods impeded any qualitative or quantitative comparison...
December 13, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29236048/adaptive-wavelet-coding-applied-in-a-wireless-control-system
#16
Felipe O S Gama, Luiz F Q Silveira, Andrés O Salazar
Wireless control systems can sense, control and act on the information exchanged between the wireless sensor nodes in a control loop. However, the exchanged information becomes susceptible to the degenerative effects produced by the multipath propagation. In order to minimize the destructive effects characteristic of wireless channels, several techniques have been investigated recently. Among them, wavelet coding is a good alternative for wireless communications for its robustness to the effects of multipath and its low computational complexity...
December 13, 2017: Sensors
https://www.readbyqxmd.com/read/29236047/hasc-and-dfat-multipotent-stem-cells-for-regenerative-medicine-a-comparison-of-their-potential-differentiation-in-vitro
#17
Marco Saler, Laura Caliogna, Laura Botta, Francesco Benazzo, Federica Riva, Giulia Gastaldi
Adipose tissue comprises both adipose and non-adipose cells such as mesenchymal stem cells. These cells show a surface antigenic profile similar to that of bone-marrow-derived MSC. The cells derived from the dedifferentiation of mature adipocytes (DFAT) are another cell population with characteristics of stemness. The aim of this study is to provide evidence of the stemness, proliferation, and differentiation of human adipose stem cells (hASC) and DFAT obtained from human subcutaneous AT and evaluate their potential use in regenerative medicine...
December 13, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29236046/advances-in-proteomic-techniques-for-cytokine-analysis-focus-on-melanoma-research
#18
REVIEW
Helena Kupcova Skalnikova, Jana Cizkova, Jakub Cervenka, Petr Vodicka
Melanoma is a skin cancer with permanently increasing incidence and resistance to therapies in advanced stages. Reports of spontaneous regression and tumour infiltration with T-lymphocytes makes melanoma candidate for immunotherapies. Cytokines are key factors regulating immune response and intercellular communication in tumour microenvironment. Cytokines may be used in therapy of melanoma to modulate immune response. Cytokines also possess diagnostic and prognostic potential and cytokine production may reflect effects of immunotherapies...
December 13, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29236042/single-channel-eeg-artifact-identification-using-two-dimensional-multi-resolution-analysis
#19
Mojtaba Taherisadr, Omid Dehzangi, Hossein Parsaei
As a diagnostic monitoring approach, electroencephalogram (EEG) signals can be decoded by signal processing methodologies for various health monitoring purposes. However, EEG recordings are contaminated by other interferences, particularly facial and ocular artifacts generated by the user. This is specifically an issue during continuous EEG recording sessions, and is therefore a key step in using EEG signals for either physiological monitoring and diagnosis or brain-computer interface to identify such artifacts from useful EEG components...
December 13, 2017: Sensors
https://www.readbyqxmd.com/read/29235971/western-blot-analysis-of-cells-encapsulated-in-self-assembling-peptide-hydrogels
#20
Kyle A Burgess, Aline F Miller, Delvac Oceandy, Alberto Saiani
Continuous optimization of in vitro analytical techniques is ever more important, especially given the development of new materials for tissue engineering studies. In particular, isolation of cellular components for downstream applications is often hindered by the presence of biomaterials, presenting a major obstacle in understanding how cell-matrix interactions influence cell behavior. Here, we describe an approach for western blot analysis of cells that have been encapsulated in self-assembling peptide hydrogels (SAPHs), which highlights the need for complete solubilization of the hydrogel construct...
December 1, 2017: BioTechniques
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