keyword
MENU ▼
Read by QxMD icon Read
search

Nucleolar pattern

keyword
https://www.readbyqxmd.com/read/27871485/unusual-processing-generates-spa-lncrnas-that-sequester-multiple-rna-binding-proteins
#1
Huang Wu, Qing-Fei Yin, Zheng Luo, Run-Wen Yao, Chuan-Chuan Zheng, Jun Zhang, Jian-Feng Xiang, Li Yang, Ling-Ling Chen
We identify a type of polycistronic transcript-derived long noncoding RNAs (lncRNAs) that are 5' small nucleolar RNA (snoRNA) capped and 3' polyadenylated (SPAs). SPA processing is associated with nascent mRNA 3' processing and kinetic competition between XRN2 trimming and Pol II elongation. Following cleavage/polyadenylation of its upstream gene, the downstream uncapped pre-SPA is trimmed by XRN2 until this exonuclease reaches the co-transcriptionally assembled snoRNP. This snoRNP complex prevents further degradation, generates a snoRNA 5' end, and allows continuous Pol II elongation...
November 3, 2016: Molecular Cell
https://www.readbyqxmd.com/read/27852862/adeno-associated-virus-assembly-activating-protein-is-not-an-essential-requirement-for-capsid-assembly-of-aav-serotypes-4-5-and-11
#2
Lauriel F Earley, John M Powers, Kei Adachi, Joshua T Baumgart, Nancy L Meyer, Qing Xie, Michael S Chapman, Hiroyuki Nakai
: Adeno-associated virus (AAV) vectors have made great progress in their use for gene therapy; however, fundamental aspects of AAV's capsid assembly remain poorly characterized. In this regard, the discovery of assembly-activating protein (AAP) sheds new light on this crucial part of AAV biology and vector production. Previous studies have shown AAP to be essential for assembly; however, how its mechanistic roles in assembly might differ among AAV serotypes remains uncharacterized. Here, we show that biological properties of AAPs and capsid assembly processes are surprisingly distinct among the AAV serotypes 1 to 12...
November 16, 2016: Journal of Virology
https://www.readbyqxmd.com/read/27795408/the-nonstructural-protein-nss-of-schmallenberg-virus-is-targeted-to-the-nucleolus-and-induces-nucleolar-disorganization
#3
Julie Gouzil, Aurore Fablet, Estelle Lara, Grégory Caignard, Marielle Cochet, Cindy Kundlacz, Massimo Palmarini, Mariana Varela, Emmanuel Breard, Corinne Sailleau, Cyril Viarouge, Muriel Coulpier, Stéphan Zientara, Damien Vitour
: Schmallenberg virus (SBV) was discovered in Germany in late 2011 and then spread rapidly to many European countries. SBV is an orthobunyavirus that causes abortion and congenital abnormalities in ruminants. A virus-encoded non-structural protein, termed NSs, is a major virulence factor of SBV and it is known to promote the degradation of Rpb1, a subunit of the RNA Pol II complex, and therefore hampers global cellular transcription. In this study we found that NSs is mainly localized in the nucleus of infected cells, and, specifically appears to target the nucleolus through a nucleolar localization signal (NoLS) localized between residues 33 and 51 of the protein...
October 19, 2016: Journal of Virology
https://www.readbyqxmd.com/read/27790225/cytomolecular-analysis-of-ribosomal-dna-evolution-in-a-natural-allotetraploid-brachypodium-hybridum-and-its-putative-ancestors-dissecting-complex-repetitive-structure-of-intergenic-spacers
#4
Natalia Borowska-Zuchowska, Miroslaw Kwasniewski, Robert Hasterok
Nucleolar dominance is an epigenetic phenomenon associated with nuclear 35S rRNA genes and consists in selective suppression of gene loci inherited from one of the progenitors in the allopolyploid. Our understanding of the exact mechanisms that determine this process is still fragmentary, especially in case of the grass species. This study aimed to shed some light on the molecular basis of this genome-specific inactivation of 35S rDNA loci in an allotetraploid Brachypodium hybridum (2n = 30), which arose from the interspecific hybridization between two diploid ancestors that were very similar to modern B...
2016: Frontiers in Plant Science
https://www.readbyqxmd.com/read/27728911/nucleolar-dominance-and-repression-of-45s-ribosomal-rna-genes-in-hybrids-between-xenopus-borealis-and-x-muelleri-2n-36
#5
Sebastian Maciak, Katarzyna Michalak, Shiv D Kale, Pawel Michalak
Nucleolar dominance is a dramatic disruption in the formation of nucleoli and the expression of ribosomal RNA (rRNA) genes, characteristic of some plant and animal hybrids. Here, we report that F1 hybrids produced from reciprocal crosses between 2 sister species of Xenopus clawed frogs, X. muelleri and X. borealis, undergo nucleolar dominance somewhat distinct from a pattern previously reported in hybrids between phylogenetically more distant Xenopus species. Patterns of nucleolar development, 45S rRNA expression, and gene copy inheritance were investigated using a combination of immunostaining, pyrosequencing, droplet digital PCR, flow cytometry, and epigenetic inhibition...
October 12, 2016: Cytogenetic and Genome Research
https://www.readbyqxmd.com/read/27707747/gene-conversion-events-and-variable-degree-of-homogenization-of-rdna-loci-in-cultivars-of-brassica-napus
#6
Jana Sochorová, Olivier Coriton, Alena Kuderová, Jana Lunerová, Anne-Marie Chèvre, Aleš Kovařík
BACKGROUND AND AIMS: Brassica napus (AACC, 2n = 38, oilseed rape) is a relatively recent allotetraploid species derived from the putative progenitor diploid species Brassica rapa (AA, 2n = 20) and Brassica oleracea (CC, 2n = 18). To determine the influence of intensive breeding conditions on the evolution of its genome, we analysed structure and copy number of rDNA in 21 cultivars of B. napus, representative of genetic diversity. METHODS: We used next-generation sequencing genomic approaches, Southern blot hybridization, expression analysis and fluorescence in situ hybridization (FISH)...
October 5, 2016: Annals of Botany
https://www.readbyqxmd.com/read/27481902/ethnic-differences-in-autoantibody-diversity-and-hierarchy-more-clues-from-a-us-cohort-of-patients-with-systemic-sclerosis
#7
Sarada L Nandiwada, Lisa K Peterson, Maureen D Mayes, Troy D Jaskowski, Elisabeth Malmberg, Shervin Assassi, Minoru Satoh, Anne E Tebo
OBJECTIVE: To determine the autoantibody repertoire and clinical associations in a multiethnic cohort of American patients with systemic sclerosis (SSc). METHODS: There were 1000 patients with SSc (196 Hispanic, 228 African American, 555 white, and 21 other) who were screened for antinuclear antibodies (ANA), including anticentromere antibodies (ACA) by indirect immunofluorescence assay, antitopoisomerase-1 (topo-1/Scl-70) by immunodiffusion, and anti-RNA polymerase III (RNAP III) by ELISA...
October 2016: Journal of Rheumatology
https://www.readbyqxmd.com/read/27473587/clinical-significance-of-histone-demethylase-no66-in-invasive-colorectal-cancer
#8
Yujiro Nishizawa, Naohiro Nishida, Masamitsu Konno, Koichi Kawamoto, Ayumu Asai, Jun Koseki, Hidekazu Takahashi, Naotsugu Haraguchi, Junichi Nishimura, Taishi Hata, Chu Matsuda, Tsunekazu Mizushima, Taroh Satoh, Yuichiro Doki, Masaki Mori, Hideshi Ishii
BACKGROUND: Targeting epigenetic regulators is a promising therapeutic strategy against cancer. However, because of the broad spectrum of targets, selective inhibition of cancer-associated genes remains a major challenge. To address this issue, we focused on the oncogene-regulated histone demethylase, nucleolar protein 66 (NO66 [C14orf169/MAPJD]), which is known to work coordinately with the well-characterized oncogene, c-MYC. METHODS: To investigate expression patterns and clinical significance of NO66 in colorectal cancer (CRC), we performed immunohistochemical staining in 114 CRC cases...
July 29, 2016: Annals of Surgical Oncology
https://www.readbyqxmd.com/read/27252267/development-and-multi-center-evaluation-of-a-novel-immunoadsorption-method-for-anti-dfs70-antibodies
#9
C Bentow, R Rosenblum, P Correia, E Karayev, D Karayev, D Williams, J Kulczycka, M J Fritzler, M Mahler
OBJECTIVE: Antinuclear antibodies (ANA) represent a hallmark in the diagnosis of ANA-associated rheumatic diseases (AARD). However, anti-DFS70 antibodies are present in a higher portion of the healthy individuals (HI) than in patients with AARD. Consequently, we developed a novel, highly specific indirect immunofluorescence (IIF) method that blocks anti-DFS70 antibodies from binding to HEp-2 cells and to evaluate the method in a multi-center study. METHODS: A total of 18 samples from systemic lupus erythematosus patients (SLE, n = 7) and HI (n = 11) were used for the initial development of the immunoadsorption method...
July 2016: Lupus
https://www.readbyqxmd.com/read/27187571/spatial-segregation-of-heterochromatin-uncovering-functionality-in-a-multicellular-organism
#10
Daphne S Cabianca, Susan M Gasser
Multiple layers of regulation are required to ensure appropriate patterns of gene expression for accurate cell differentiation. Interphase chromatin is non-randomly distributed within the nucleus, with highly compacted, transcriptionally silent heterochromatin enriched at the nuclear and nucleolar periphery. Whether this spatial organization serves a function in organismal physiology, rather than simply being a byproduct of chromatin metabolism, is a fundamental question. Recent work performed in C. elegans embryos characterized the molecular mechanisms that drive the perinuclear anchoring of heterochromatin...
May 3, 2016: Nucleus
https://www.readbyqxmd.com/read/27173106/serum-antinuclear-antibody-in-adult-thais
#11
Chanwit Prapinjumrune, Chalakorn Prucktrakul, Trakarn Sooktonglarng, Kobkan Thongprasom
OBJECTIVE: This study investigated the presence of antinuclear antibody (ANA) in older Thais compared with middle-age and younger participants. BACKGROUND: Antinuclear antibody represents the first step in the diagnostic testing for lupus erythematosus (LE) and other autoimmune diseases. Due to the lack of reference ANA levels in older, middle-age and younger Thais healthy participants, this study will be useful for determining the proper diagnostic and treatment criteria...
May 12, 2016: Gerodontology
https://www.readbyqxmd.com/read/27021793/genome-of-leptomonas-pyrrhocoris-a-high-quality-reference-for-monoxenous-trypanosomatids-and-new-insights-into-evolution-of-leishmania
#12
Pavel Flegontov, Anzhelika Butenko, Sergei Firsov, Natalya Kraeva, Marek Eliáš, Mark C Field, Dmitry Filatov, Olga Flegontova, Evgeny S Gerasimov, Jana Hlaváčová, Aygul Ishemgulova, Andrew P Jackson, Steve Kelly, Alexei Y Kostygov, Maria D Logacheva, Dmitri A Maslov, Fred R Opperdoes, Amanda O'Reilly, Jovana Sádlová, Tereza Ševčíková, Divya Venkatesh, Čestmír Vlček, Petr Volf, Jan Votýpka, Kristína Záhonová, Vyacheslav Yurchenko, Julius Lukeš
Many high-quality genomes are available for dixenous (two hosts) trypanosomatid species of the genera Trypanosoma, Leishmania, and Phytomonas, but only fragmentary information is available for monoxenous (single-host) trypanosomatids. In trypanosomatids, monoxeny is ancestral to dixeny, thus it is anticipated that the genome sequences of the key monoxenous parasites will be instrumental for both understanding the origin of parasitism and the evolution of dixeny. Here, we present a high-quality genome for Leptomonas pyrrhocoris, which is closely related to the dixenous genus Leishmania...
2016: Scientific Reports
https://www.readbyqxmd.com/read/26954256/the-hex-110-hexamerin-is-a-cytoplasmic-and-nucleolar-protein-in-the-ovaries-of-apis-mellifera
#13
Juliana Ramos Martins, Márcia Maria Gentile Bitondi
Hexamerins are insect storage proteins abundantly secreted by the larval fat body into the haemolymph. The canonical role of hexamerins consists of serving as an amino acid reserve for development toward the adult stage. However, in Apis mellifera, immunofluorescence assays coupled to confocal laser-scanning microscopy, and high-throughput sequencing, have recently shown the presence of hexamerins in other organs than the fat body. These findings have led us to study these proteins with the expectation of uncovering additional functions in insect development...
2016: PloS One
https://www.readbyqxmd.com/read/26934290/nucleolar-protein-4-like-has-a-complex-expression-pattern-in-zebrafish-embryos
#14
Supriya Borah, Praveen Barrodia, Rajeeb K Swain
The nucleolar protein 4-like (NOL4L) gene is present on chromosome 20 (20q11.21) in humans. Parts of this gene have been shown to fuse with RUNX1 and PAX5 in acute myeloid leukemia and acute lymphoblastic leukemia, respectively. The normal function of NOL4L in humans and other organisms is not well understood. The expression patterns and functions of NOL4L homologs during vertebrate development have not been reported. We sought to address these questions by studying the expression pattern of zebrafish nol4l during embryogenesis...
2016: International Journal of Developmental Biology
https://www.readbyqxmd.com/read/26934153/karyotypic-diversity-among-three-species-of-the-genus-astyanax-characiformes-characidae
#15
P B Nishiyama, M M R Vieira, F E Porto, L A Borin, A L B Portela-Castro, I C M Santos
The group Incertae sedis within the Characidae family currently includes 88 genera, previously included in the subfamily Tetragonopterinae. Among them is the genus Astyanax comprising a group of species with similar morphology and widely distributed in the Neotropics. Thus, the present study aimed to analyze the karyotype diversity in Astyanax species from different watersheds by conventional Giemsa staining, C-banding and fluorescence in situ hybridization (FISH rDNA 18S) probe.specimens of Astyanax aff. paranae belonging to the "scabripinnis complex", Astyanax asunsionensis and Astyanax aff...
June 2016: Brazilian Journal of Biology, Revista Brasleira de Biologia
https://www.readbyqxmd.com/read/26854071/high-frame-rate-resolution-of-cell-division-during-candida-albicans-filamentation
#16
Darren D Thomson, Judith Berman, Alexandra C Brand
The commensal yeast, Candida albicans, is an opportunistic pathogen in humans and forms filaments called hyphae and pseudohyphae, in which cell division requires precise temporal and spatial control to produce mononuclear cell compartments. High-frame-rate live-cell imaging (1 frame/min) revealed that nuclear division did not occur across the septal plane. We detected the presence of nucleolar fragments that may be extrachromosomal molecules carrying the ribosomal RNA genes. Cells occasionally maintained multiple nucleoli, suggesting either polyploidy, multiple nuclei and/or aneuploidy of ChrR...
March 2016: Fungal Genetics and Biology: FG & B
https://www.readbyqxmd.com/read/26846693/serological-profile-and-clinical-features-of-nucleolar-antinuclear-pattern-in-patients-with-systemic-lupus-erythematosus-from-southwestern-spain
#17
C M Cabrera, E Fernández-Grande, J M Urra
Nucleolar staining of antinuclear antibodies (ANAs) is not exclusive to patients suffering systemic sclerosis (SSc) since it can occur in other autoimmune diseases, such as systemic lupus erythematosus (SLE). The nucleolar ANA pattern presents a low incidence in patients with SLE, with less than 9% reported in some studies. The significance of nucleolar staining and antinucleolar antibodies (ANoA) in SLE is still unknown, as is its association with clinical manifestations. To address these issues, a case-control study was carried out...
August 2016: Lupus
https://www.readbyqxmd.com/read/26771299/closely-related-syntopic-cytotypes-of-astyanax-taeniatus-jenyns-1842-from-the-upper-piranga-river-upper-doce-basin-in-southeastern-brazil
#18
Marina Souza da Cunha, Vinícius José Carvalho Reis, Jorge Abdala Dergam
Astyanax taeniatus occurs in coastal areas of southeastern Brazil, and it is very abundant in the Upper Doce River Basin. Our objective was to study C-, argyrophilic nucleolar organizer region (Ag-NOR) and fluorescent in situ hybridization (FISH) banding patterns using 5S, 18S, CA(15), and GA(15) repetitive DNA probes on a population of A. taeniatus present in the Piranga River, in the Doce Basin. Two syntopic cytotypes were found, both with 2n = 50: cytotype A (14m + 12sm + 16st + 8t) and cytotype B (10m + 14sm + 18st + 8t)...
April 2016: Zebrafish
https://www.readbyqxmd.com/read/26749075/rna-synthesis-and-turnover-in-the-molluscan-nervous-system-studied-by-click-it-method
#19
Victor N Ierusalimsky, Pavel M Balaban
RNA synthesis can be detected by means of the in vivo incorporation of 5-ethynyluridine (EU) in newly-synthesized RNA with the relatively simple Click-iT method. We used this method to study the RNA synthesis in the CNS tissue of adult and juvenile terrestrial snails Helix lucorum L. Temporally, first labeled neurons were detected in the adult CNS after 4-h of isolated CNS incubation in EU solution, while 12-h of incubation led to extensive labeling of most CNS neurons. The EU labeling was present as the nuclear and nucleolar staining...
February 15, 2016: Brain Research
https://www.readbyqxmd.com/read/26740135/brief-review-of-the-chicken-major-histocompatibility-complex-the-genes-their-distribution-on-chromosome-16-and-their-contributions-to-disease-resistance
#20
REVIEW
Marcia M Miller, Robert L Taylor
Nearly all genes presently mapped to chicken chromosome 16 (GGA 16) have either a demonstrated role in immune responses or are considered to serve in immunity by reason of sequence homology with immune system genes defined in other species. The genes are best described in regional units. Among these, the best known is the polymorphic major histocompatibility complex-B (MHC-B) region containing genes for classical peptide antigen presentation. Nearby MHC-B is a small region containing two CD1 genes, which encode molecules known to bind lipid antigens and which will likely be found in chickens to present lipids to specialized T cells, as occurs with CD1 molecules in other species...
February 2016: Poultry Science
keyword
keyword
114788
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"