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https://www.readbyqxmd.com/read/29590648/calcium-calmodulin-dependent-protein-kinase-iv-mediates-ifn-%C3%AE-induced-immune-behaviors-in-skeletal-muscle-cells
#1
RuiCai Gu, MaoChao Ding, DanDan Shi, Tao Huang, MengXia Guo, Lei Yu, JiJie Hu, WenHua Huang, Hua Liao
BACKGROUND/AIMS: Whether calcium/calmodulin-dependent protein kinase IV (CaMKIV) plays a role in regulating immunologic features of muscle cells in inflammatory environment, as it does for immune cells, remains mostly unknown. In this study, we investigated the influence of endogenous CaMKIV on the immunological characteristics of myoblasts and myotubes received IFN-γ stimulation. METHODS: C2C12 and murine myogenic precursor cells (MPCs) were cultured and differentiated in vitro, in the presence of pro-inflammatory IFN-γ...
March 22, 2018: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29570076/pinpoint-delivery-of-molecules-by-using-electron-beam-addressing-virtual-cathode-display
#2
Takayuki Hoshino, Moto Yoshioka, Akira Wagatsuma, Hiroki Miyazako, Kunihiko Mabuchi
Electroporation, a physical transfection method to introduce genomic molecules in selective living cells, could be implemented by microelectrode devices. A local electric field generated by a finer electrode can induces cytomembrane poration in the electrode vicinity. To employ fine, high-speed scanning electrodes, we developed a fine virtual cathode pattern, which was generated on a cell adhesive surface of 100-nm-thick SiN membrane by inverted-electron beam lithography. The SiN membrane works as both a vacuum barrier and the display screen of the virtual cathode...
March 2018: IEEE Transactions on Nanobioscience
https://www.readbyqxmd.com/read/29558395/yak-igf2-promotes-fibroblast-proliferation-via-suppression-of-igf1r-and-pi3kcg-expression
#3
Quanwei Zhang, Qi Wang, Jishang Gong, Jiaxing Du, Yong Zhang, Xingxu Zhao
Insulin-like growth factor 2 (IGF2) recapitulates many of the activities of insulin and promotes differentiation of myoblasts and osteoblasts, which likely contribute to genetic variations of growth potential. However, little is known about the functions and signaling properties of IGF2 variants in yaks. The over-expression vector and knockdown sequence of yak IGF2 were transfected into yak fibroblasts, and the effects were detected by a series of assays. IGF2 expression in yak muscle tissues was significantly lower than that of other tissues...
March 20, 2018: Genes
https://www.readbyqxmd.com/read/29512306/preventing-muscle-wasting-by-osteoporosis-drug-alendronate-in-vitro-and-in-myopathy-models-via-sirtuin-3-down-regulation
#4
Hsien-Chun Chiu, Chen-Yuan Chiu, Rong-Sen Yang, Ding-Cheng Chan, Shing-Hwa Liu, Chih-Kang Chiang
BACKGROUND: A global consensus on the loss of skeletal muscle mass and function in humans refers as sarcopenia and cachexia including diabetes, obesity, renal failure, and osteoporosis. Despite a current improvement of sarcopenia or cachexia with exercise training and supportive therapies, alternative and specific managements are needed to discover for whom are unable or unwilling to embark on these treatments. Alendronate is a widely used drug for osteoporosis in the elderly and postmenopausal women...
March 6, 2018: Journal of Cachexia, Sarcopenia and Muscle
https://www.readbyqxmd.com/read/29508126/mir-152-regulates-the-proliferation-and-differentiation-of-c2c12-myoblasts-by-targeting-e2f3
#5
Mailin Gan, Jingjing Du, Linyuan Shen, Dongli Yang, Anan Jiang, Qiang Li, Yanzhi Jiang, Guoqing Tang, Mingzhou Li, Jinyong Wang, Xuewei Li, Shunhua Zhang, Li Zhu
The development of skeletal muscle is a complex process involving the proliferation, differentiation, apoptosis, and changing of muscle fiber types in myoblasts. Many reports have described the involvement of microRNAs in the myogenesis of myoblasts. In this study, we found that the expression of miR-152 was gradually down-regulated during myoblast proliferation, but gradually up-regulated during the differentiation of myoblasts. Transfection with miR-152 mimics restrained cell proliferation and decreased the expression levels of cyclin E, CDK4, and cyclin D1, but promoted myotube formation and significantly increased the mRNA expression levels of MyHC, MyoD, MRF4, and MyoG in C2C12 myoblasts...
March 5, 2018: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/29505923/mir-204-5p-regulates-c2c12-myoblast-differentiation-by-targeting-mef2c-and-err%C3%AE
#6
Xiao Cheng, Jingjing Du, Linyuan Shen, Zhendong Tan, Dongmei Jiang, Anan Jiang, Qiang Li, Guoqing Tang, Yanzhi Jiang, Jinyong Wang, Xuewei Li, Shunhua Zhang, Li Zhu
Myogenic differentiation, which occurs in the process of muscle development, is a highly ordered process. Increasing evidence indicates that microRNAs (miRNAs) are important regulators in myogenic processes. In this study, we found that miR-204-5p expression gradually decreased when myoblasts were induced to differentiate. Our results suggested that miR-204-5p blunted myoblast differentiation, which was accompanied with a decreased proportion of myosin heavy chain (MyHC)-positive cells in myoblasts with augmented expression of miR-204-5p...
March 1, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29328071/sumoylation-of-histone-deacetylase-1-regulates-myod-signaling-during-myogenesis
#7
Hosouk Joung, Sehee Kwon, Kyoung-Hoon Kim, Yun-Gyeong Lee, Sera Shin, Duk-Hwa Kwon, Yeong-Un Lee, Taewon Kook, Nakwon Choe, Jeong Chul Kim, Young-Kook Kim, Gwang Hyeon Eom, Hyun Kook
Sumoylation, the conjugation of a small ubiquitin-like modifier (SUMO) protein to a target, has diverse cellular effects. However, the functional roles of the SUMO modification during myogenesis have not been fully elucidated. Here, we report that basal sumoylation of histone deacetylase 1 (HDAC1) enhances the deacetylation of MyoD in undifferentiated myoblasts, whereas further sumoylation of HDAC1 contributes to switching its binding partners from MyoD to Rb to induce myocyte differentiation. Differentiation in C2C12 skeletal myoblasts induced new immunoblot bands above HDAC1 that were gradually enhanced during differentiation...
January 12, 2018: Experimental & Molecular Medicine
https://www.readbyqxmd.com/read/29215104/a-novel-ptp1b-inhibitor-extracted-from-ganoderma-lucidum-ameliorates-insulin-resistance-by-regulating-irs1-glut4-cascades-in-the-insulin-signaling-pathway
#8
Zhou Yang, Fan Wu, Yanming He, Qiang Zhang, Yuan Zhang, Guangrong Zhou, Hongjie Yang, Ping Zhou
Insulin resistance caused by the overexpression of protein tyrosine phosphatase 1 B (PTP1B) as well as the dephosphorylation of its target is one of the main causes of type 2 diabetes (T2D). A newly discovered proteoglycan, Fudan-Yueyang Ganoderma lucidum (FYGL) extracted from Ganoderma lucidum, was first reported to be capable of competitively inhibiting PTP1B activity in vitro in our previous work. In the present study, we sought to reveal the mechanism of PTP1B inhibition by FYGL at the animal and cellular levels...
January 24, 2018: Food & Function
https://www.readbyqxmd.com/read/29207028/role-of-the-cofilin-2-gene-in-regulating-the-myosin-heavy-chain-genes-in-mouse-myoblast-c2c12-cells
#9
Hongyan Zhu, Huixin Yang, Song Zhao, Junfeng Zhang, Dan Liu, Yumin Tian, Zhiyi Shen, Yuhong Su
The cofilin 2 (CFL2) and myosin heavy chain (MyHC) genes play a key role in muscle development and myofibrillar formation. The aim of the present study was to investigate the effect of CFL2 on genes involved in fiber formation and the mechanisms underlying this process. Undifferentiated and differentiated C2C12 cells (UDT and DT, respectively) were transfected with CFL2 small interfering RNA (siRNA). CFL2 mRNA and protein levels were assessed using reverse transcription polymerase chain reaction (RT-PCR) and western blotting, respectively...
February 2018: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/29161332/osteoglycin-inhibition-by-microrna-mir-155-impairs-myogenesis
#10
Paula Paccielli Freire, Sarah Santiloni Cury, Grasieli de Oliveira, Geysson Javier Fernandez, Leonardo Nazario Moraes, Bruno Oliveira da Silva Duran, Juarez Henrique Ferreira, César Seigi Fuziwara, Edna Teruko Kimura, Maeli Dal-Pai-Silva, Robson Francisco Carvalho
Skeletal myogenesis is a regulated process in which mononucleated cells, the myoblasts, undergo proliferation and differentiation. Upon differentiation, the cells align with each other, and subsequently fuse to form terminally differentiated multinucleated myotubes. Previous reports have identified the protein osteoglycin (Ogn) as an important component of the skeletal muscle secretome, which is expressed differentially during muscle development. However, the posttranscriptional regulation of Ogn by microRNAs during myogenesis is unknown...
2017: PloS One
https://www.readbyqxmd.com/read/29113462/simultaneous-transplantation-of-fetal-ventral-mesencephalic-tissue-and-encapsulated-genetically-modified-cells-releasing-gdnf-in-a-hemi-parkinsonian-rat-model-of-parkinson-s-disease
#11
Alberto Perez-Bouza, Stefano Di Santo, Stefanie Seiler, Morten Meyer, Lukas Andereggen, Alexander Huber, Raphael Guzman, Hans R Widmer
Transplantation of fetal ventral mesencephalic (VM) neurons for Parkinson's disease (PD) is limited by poor survival and suboptimal integration of grafted tissue into the host brain. In a 6-hydroxydopamine rat model of PD, we investigated the feasibility of simultaneous transplantation of rat fetal VM tissue and polymer-encapsulated C2C12 myoblasts genetically modified to produce glial cell line-derived neurotrophic factor (GDNF) or mock-transfected myoblasts on graft function. Amphetamine-induced rotations were assessed prior to transplantation and 2, 4, 6 and 9 wk posttransplantation...
September 2017: Cell Transplantation
https://www.readbyqxmd.com/read/29107054/selecting-the-correct-cellular-model-for-assessing-of-the-biological-response-of-collagen-based-biomaterials
#12
Natalia Davidenko, Samir Hamaia, Daniel V Bax, Jean-Daniel Malcor, Carlos F Schuster, Donald Gullberg, Richard W Farndale, Serena M Best, Ruth E Cameron
Accurate evaluation of the biological performance of biomaterials requires the correct assessment of their native-like cell ligation properties. However, cell attachment studies often overlook the details of the substrate-cell binding mechanisms, be they integrin-mediated or non-specific, and ignore the class- and species-specificities of the cell adhesion receptor involved. In this work we have used different collagen (Col) substrates (fibrillar collagens I, II and III and network-forming Col IV), containing different affinity cell-recognition motifs, to establish the influence of the receptor identity and species-specificity on collagen-cell interactive properties...
January 2018: Acta Biomaterialia
https://www.readbyqxmd.com/read/29080903/human-endoglin-as-a-potential-new-partner-involved-in-platelet-endothelium-interactions
#13
Elisa Rossi, Miguel Pericacho, Christilla Bachelot-Loza, Dominique Pidard, Pascale Gaussem, Sonia Poirault-Chassac, Francisco J Blanco, Carmen Langa, Consuelo González-Manchón, Jose M Lopez Novoa, David M Smadja, Carmelo Bernabeu
Complex interactions between platelets and activated endothelium occur during the thrombo-inflammatory reaction at sites of vascular injuries and during vascular hemostasis. The endothelial receptor endoglin is involved in inflammation through integrin-mediated leukocyte adhesion and transmigration; and heterozygous mutations in the endoglin gene cause hereditary hemorrhagic telangiectasia type 1. This vascular disease is characterized by a bleeding tendency that is postulated to be a consequence of telangiectasia fragility rather than a platelet defect, since platelets display normal functions in vitro in this condition...
October 28, 2017: Cellular and Molecular Life Sciences: CMLS
https://www.readbyqxmd.com/read/29021711/ginsenoside-rg1-from-panax-ginseng-enhances-myoblast-differentiation-and-myotube-growth
#14
Ga-Yeon Go, Sang-Jin Lee, Ayoung Jo, Jaecheol Lee, Dong-Wan Seo, Jong-Sun Kang, Si-Kwan Kim, Su-Nam Kim, Yong Kee Kim, Gyu-Un Bae
BACKGROUND: Ginsenoside Rg1 belongs to protopanaxatriol-type ginsenosides and has diverse pharmacological activities. In this report, we investigated whether Rg1 could upregulate muscular stem cell differentiation and muscle growth. METHODS: C2C12 myoblasts, MyoD-transfected 10T1/2 embryonic fibroblasts, and HEK293T cells were treated with Rg1 and differentiated for 2 d, subjected to immunoblotting, immunocytochemistry, or immunoprecipitation. RESULTS: Rg1 activated promyogenic kinases, p38MAPK (mitogen-activated protein kinase) and Akt signaling, that in turn promote the heterodimerization with MyoD and E proteins, resulting in enhancing myogenic differentiation...
October 2017: Journal of Ginseng Research
https://www.readbyqxmd.com/read/28993734/osmotin-protects-h9c2-cells-from-simulated-ischemia-reperfusion-injury-through-adipor1-pi3k-akt-signaling-pathway
#15
Jianhua Liu, Hua Sui, Jianlin Zhao, Yan Wang
Objective: This study aimed to investigate the effect of osmotin on myocardial ischemia/reperfusion (I/R), as well as the underlying mechanisms. Methods:In vitro I/R injury model was established on rat cardiac myoblast H9c2 cells by oxygen and glucose deprivation followed by reperfusion (OGD/R). Cells were administrated with osmotin, and transfected with small interfering RNAs (siRNAs) which specifically target adiponectin receptor 1 or 2 (AdipoR1/2). Besides, the cells were incubated with or without LY294002 as inhibitor of phosphatidylinositol 3-kinase (PI3K) under OGD/R condition...
2017: Frontiers in Physiology
https://www.readbyqxmd.com/read/28992079/sustained-activation-of-p2x7-induces-mmp-2-evoked-cleavage-and-functional-purinoceptor-inhibition
#16
Christopher N J Young, Natalia Chira, Justyna Róg, Rasha Al-Khalidi, Magalie Benard, Ludovic Galas, Philippe Chan, David Vaudry, Krzysztof Zablocki, Dariusz C Górecki
P2X7 purinoceptor promotes survival or cytotoxicity depending on extracellular adenosine triphosphate (ATP) stimulus intensity controlling its ion channel or P2X7-dependent large pore (LP) functions. Mechanisms governing this operational divergence and functional idiosyncrasy are ill-understood. We have discovered a feedback loop where sustained activation of P2X7 triggers release of active matrix metalloproteinase 2 (MMP-2), which halts ion channel and LP responses via the MMP-2-dependent receptor cleavage...
August 18, 2017: Journal of Molecular Cell Biology
https://www.readbyqxmd.com/read/28981327/molecular-cloning-and-motif-identification-of-the-sheep-musclin-gene-promoter
#17
Weizhen Li, Hongqiang Zhang, Shuai Qi, Jian Qin, Hong Guan, Jianwei Li, Xiaorong An, Rong Du
Musclin is a bioactive factor that functions in regulating the muscle growth and metabolism. To investigate the transcriptional regulatory mechanism of the gene, the 1.4 kb musclin promoter in sheep was cloned (GenBank accession: JX966391) and the sequence was analyzed to predict the motifs associated with muscle growth. Next the enhanced green fluorescent protein (EGFP) was selected as the reporter gene and various wild-type and motif-mutant vectors were constructed. The transcriptional regulatory activities were compared by observing the fluorescence strength and detecting the EGFP mRNA expression in C2C12 myoblasts transfected with the vectors...
October 5, 2017: DNA and Cell Biology
https://www.readbyqxmd.com/read/28951939/biological-and-pro-angiogenic-properties-of-genetically-modified-human-primary-myoblasts-overexpressing-placental-growth-factor-in-in-vitro-and-in-vivo-studies
#18
Agnieszka Zimna, Bartosz Wiernicki, Tomasz Kolanowski, Natalia Rozwadowska, Agnieszka Malcher, Wojciech Labedz, Tomasz Trzeciak, Katarzyna Chojnacka, Katarzyna Bednarek-Rajewska, Przemyslaw Majewski, Maciej Kurpisz
Cardiovascular diseases are a growing problem in developing countries; therefore, there is an ongoing intensive search for new approaches to treat these disorders. Currently, cellular therapies are focused on healing the damaged heart by implanting stem cells modified with pro-angiogenic factors. This approach ensures that the introduced cells are capable of fulfilling the complex requirements of the environment, including the replacement of the post-infarction scar with cells that are able to contract and promote the formation of new blood vessels that can supply the ischaemic region with nutrients and oxygen...
September 26, 2017: Archivum Immunologiae et Therapiae Experimentalis
https://www.readbyqxmd.com/read/28919162/mir-101-1-expression-pattern-in-qinchuan-cattle-and-its-role-in-the-regulation-of-cell-differentiation
#19
Jiyao Wu, Dandan He, Binglin Yue, Chunlei Zhang, Xingtang Fang, Hong Chen
MiRNAs have emerged as key regulators of skeletal muscle development, but the knowledge of miRNAs in the molecular network of muscle development remains poorly understood. In this study, we designed to examine the biological function of bovine-miR-101-1. The bovine miR-101-1 was detected in the skeletal muscle of fetal, calf and adult cattle. Its abundance was significantly higher in the skeletal muscle of calf cattle than that in fetal and adult cattle. In the course of C2C12 myoblast differentiation, the expression of miR-101-1 gradually increased...
December 15, 2017: Gene
https://www.readbyqxmd.com/read/28803542/spin-infection-enables-efficient-gene-delivery-to-muscle-stem-cells
#20
Yusaku Kodaka, Yoko Asakura, Atsushi Asakura
Viral vector-mediated foreign gene expression in cultured cells has been extensively used in stem cell studies to explore gene function. However, it is difficult to obtain high-quality stem cells and primary cells after viral vector infection. Here, we describe a new protocol for high-efficiency retroviral infection of primary muscle stem cell (satellite cell) cultures. We compared multiple commercially available transfection reagents to determine which was optimal for retroviral infections of primary myoblasts...
August 1, 2017: BioTechniques
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