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cardiac function mitochondria

Rinat A Mukhamadiyarov, Evgeniya A Senokosova, Sergey S Krutitsky, Darya V Voevoda, Inna A Pyshnaya, Vladimir V Ivanov, Martin J Lewis, Igor Khaliulin
Liposomes have the potential to be used for drug delivery. Meanwhile, liposome size may affect their accumulation in the target tissue. We investigated the myocardial accumulation of two populations of liposomes (∼70 and 110 nm diameter) during ischaemia and their effect on ischaemia/reperfusion injury.Isolated rat hearts were subjected to 30 min low-flow ischemia with the liposomes followed by 30 min liposome-free reperfusion. The liposomes were loaded with the fluorescent dye Nile Red to assess their accumulation in myocardium...
May 28, 2018: Journal of Cardiovascular Pharmacology
Navratan Bagwan, Elena Bonzon-Kulichenko, Enrique Calvo, Ana Victoria Lechuga-Vieco, Spiros Michalakopoulos, Marco Trevisan-Herraz, Iakes Ezkurdia, José Manuel Rodríguez, Ricardo Magni, Ana Latorre-Pellicer, José Antonio Enríquez, Jesús Vázquez
Post-translational modifications hugely increase the functional diversity of proteomes. Recent algorithms based on ultratolerant database searching are forging a path to unbiased analysis of peptide modifications by shotgun mass spectrometry. However, these approaches identify only one-half of the modified forms potentially detectable and do not map the modified residue. Moreover, tools for the quantitative analysis of peptide modifications are currently lacking. Here, we present a suite of algorithms that allows comprehensive identification of detectable modifications, pinpoints the modified residues, and enables their quantitative analysis through an integrated statistical model...
June 19, 2018: Cell Reports
Cristiane Cecatto, Alessandro Wajner, Carmen Regla Vargas, Simone Magagnin Wajner, Alexandre Umpierrez Amaral, Moacir Wajner
Patients affected by long-chain 3-hydroxyacyl-CoA dehydrogenase (LCHAD) deficiency predominantly present severe liver and cardiac dysfunction, as well as neurological symptoms during metabolic crises, whose pathogenesis is still poorly known. In this study, we demonstrate for the first time that pathological concentrations of 3-hydroxypalmitic acid (3HPA), the long-chain hydroxyl fatty acid (LCHFA) that most accumulates in LCHAD deficiency, significantly decreased adenosine triphosphate-linked and uncoupled mitochondrial respiration in intact cell systems consisting of heart fibers, cardiomyocytes, and hepatocytes, but less intense in diced forebrain...
June 20, 2018: Journal of Cellular Biochemistry
Edoardo Bertero, Christoph Maack
The heart consumes large amounts of energy in the form of ATP that is continuously replenished by oxidative phosphorylation in mitochondria and, to a lesser extent, by glycolysis. To adapt the ATP supply efficiently to the constantly varying demand of cardiac myocytes, a complex network of enzymatic and signalling pathways controls the metabolic flux of substrates towards their oxidation in mitochondria. In patients with heart failure, derangements of substrate utilization and intermediate metabolism, an energetic deficit, and oxidative stress are thought to underlie contractile dysfunction and the progression of the disease...
June 18, 2018: Nature Reviews. Cardiology
Dharendra Thapa, Michael W Stoner, Manling Zhang, Bingxian Xie, Janet R Manning, Danielle Guimaraes, Sruti Shiva, Michael J Jurczak, Iain Scott
Mitochondria supply ~90% of the ATP required for contractile function in cardiac cells. While adult cardiomyocytes preferentially utilize fatty acids as a fuel source for oxidative phosphorylation, cardiac mitochondria can switch to other substrates when required. This change is driven in part by a combination of extracellular and intracellular signal transduction pathways that alter mitochondrial gene expression and enzymatic activity. The mechanisms by which extracellular metabolic information is conveyed to cardiac mitochondria are not currently well defined...
June 9, 2018: Redox Biology
Swati Dey, Deeptankar DeMazumder, Agnieszka Sidor, D B Foster, Brian O'Rourke
<u>Rationale:</u> Despite increasing prevalence and incidence of heart failure (HF), therapeutic options remain limited. In early stages of HF, sudden cardiac death (SCD) from ventricular arrhythmias claims many lives. Reactive oxygen species (ROS) have been implicated in both arrhythmias and contractile dysfunction. However, little is known about how ROS in specific subcellular compartments contribute to HF or SCD pathophysiology. The role of ROS in chronic proteome remodeling has not been explored...
June 13, 2018: Circulation Research
Kristin M O'Brien, Anna S Rix, Stuart Egginton, Anthony P Farrell, Elizabeth L Crockett, Karen Schlauch, Rebekah Woolsey, Megan Hoffman, Sean Merriman
Studies in temperate fishes provide evidence that cardiac mitochondrial function and the capacity to fuel cardiac work contributes to thermal tolerance. Here we tested the hypothesis that decreased cardiac aerobic metabolic capacity contributes to the lower thermal tolerance of the haemoglobinless Antarctic icefish, Chaenocephalus aceratus , compared to the red-blooded Antarctic species, Notothenia coriiceps. Maximal activities of citrate synthase (CS) and lactate dehydrogenase (LDH), respiration rates of isolated mitochondria, adenylate levels, and changes in mitochondrial protein expression were quantified from hearts of animals held at ambient temperature or exposed to their critical thermal maximum (CTMAX )...
June 12, 2018: Journal of Experimental Biology
Einat Bigelman, Lena Cohen, Genya Aharon-Hananel, Ran Levy, Zach Rozenbaum, Ann Saada, Gad Keren, Michal Entin-Meer
BACKGROUND: Mitochondria hold crucial importance in organs with high energy demand especially the heart. We investigated whether chronic kidney disease (CKD), which eventually culminates in cardiorenal syndrome, could affect cardiac mitochondria and assessed the potential involvement of angiotensin II (AngII) in the process. METHODS: Male Lewis rats underwent 5/6 nephrectomy allowing CKD development for eight months or for eleven weeks. Short-term CKD rats were administered with AngII receptor blocker (ARB)...
2018: PloS One
Dongshi Liang, Airong Huang, Yimei Jin, Miaomiao Lin, Xiaojiao Xia, Xiaoli Chen, Airong Huang
This study aimed to examine whether exogenous NaHS can protect myocardial mitochondrial injury from sepsis by enhancing the peroxisome proliferator-activated receptor gamma co-activator 1α (PGC-1α)/ nuclear factor erythroid-2-related factor 2 (NRF2) pathway and mitochondrial biosynthesis in mice. Animals were divided into sham-operated, sepsis, sepsis + 25 μmol/L NaHS, sepsis + 50 μmol/L NaHS, sepsis + 100 μmol/L NaHS, and sepsis + 200 μmol/L NaHS groups. The myocardial damage was evaluated by hematoxylin and eosin staining for myocardial microstructure and serum cardiac troponin I (cTnI) detection...
2018: American Journal of Translational Research
Wenjun Xiong, Jinghai Hua, Zuheng Liu, Wanqiang Cai, Yujia Bai, Qiong Zhan, Wenyan Lai, Qingchun Zeng, Hao Ren, Dingli Xu
BACKGROUND: Mitochondrial quality control is crucial to the development of angiotensin II (AngII)-induced cardiac hypertrophy. PTEN induced putative kinase 1 (PINK1) is rapidly degraded in normal mitochondria but accumulates in damaged mitochondria, triggering autophagy to protect cells. PINK1 mediates mitophagy in general, but whether PINK1 mediates AngII-induced mitophagy and the effects of PINK1 on AngII-induced injury are unknown. This study was designed to investigate the function of PINK1 in an AngII stimulation model and its regulation of AngII-induced mitophagy...
September 1, 2018: International Journal of Cardiology
Irina Odinokova, Yulia Baburina, Alexey Kruglov, Irina Fadeeva, Alena Zvyagina, Linda Sotnikova, Vladimir Akatov, Olga Krestinina
Excessive generation of reactive oxygen species (ROS) in mitochondria and the opening of the nonselective mitochondrial permeability transition pore are important factors that promote cardiac pathologies and dysfunction. The hormone melatonin (MEL) is known to improve the functional state of mitochondria via an antioxidant effect. Here, the effect of MEL administration on heart mitochondria from aged rats with acute cardiac failure caused by isoprenaline hydrochloride (ISO) was studied. A histological analysis revealed that chronic intake of MEL diminished the age-dependent changes in the structure of muscle fibers of the left ventricle, muscle fiber swelling, and injury zones characteristic of acute cardiac failure caused by ISO...
May 23, 2018: International Journal of Molecular Sciences
Tik-Chee Cheng, Jennifer L Philip, Diana Marcela Tabima, Timothy A Hacker, Naomi C Chesler
Right ventricular failure (RVF) is the major cause of death in pulmonary hypertension. Recent studies have characterized changes in RV structure in RVF, including hypertrophy, fibrosis and abnormalities in mitochondria. Few if any studies have explored the relationships between these multi-scale structural changes and functional changes in RVF. Pulmonary artery banding (PAB) was used to induce RVF due to pressure overload in male rats. Eight-weeks post-surgery, terminal invasive measurements demonstrated RVF with decreased ejection fraction (70{plus minus}10% vs 45{plus minus}15%, Sham vs...
June 8, 2018: American Journal of Physiology. Heart and Circulatory Physiology
Gabriel Heras, Arvind Venkat Namuduri, Leonardo Traini, Ganna Shevchenko, Alexander Falk, Sara Bergström Lind, Jia Mi, Geng Tian
The Muscle RING-finger protein-1 (MuRF1) is an E3 ubiquitin ligase expressed in skeletal and cardiac muscle tissues and it plays important roles in muscle remodeling. Upregulation of MuRF1 gene transcription participates in skeletal muscle atrophy, on contrary downregulation of protein expression leads to cardiac hypertrophy. MuRF1 gene point mutations have been found to generate protein aggregate myopathies (PAMs) defined as muscle disorder characterized by protein accumulation in muscle fibers. We have discovered that MuRF1 turned out to be also a target for a new post-translational modification arbitrated by conjugation of SUMO-1 and it is mediated by the SUMO ligases E2 UBC9 and the E3 PIASγ/4...
June 4, 2018: Journal of Molecular Cell Biology
Jing-Yi Zhang, Min Wang, Rui-Ying Wang, Xiao Sun, Yu-Yang Du, Jing-Xue Ye, Gui-Bo Sun, Xiao-Bo Sun
Arsenic trioxide (ATO) is used as a therapeutic agent in the treatment of acute promyelocytic leukemia (APL). The therapeutic use of arsenic is limited due to its severe cardiovascular side effects. The cardio-protective effect of salvianolic acid A (Sal A) against ATO cardiotoxicity has been reported. However, the distinct role of the mitochondria in the cardio-protection of Sal A is not understood. The aim of this study was to determine whether Sal A preconditioning protects against ATO-induced heart injury by maintaining cardiac mitochondrial function and biogenesis...
2018: Frontiers in Pharmacology
Siva S V P Sakamuri, Jared A Sperling, Venkata N Sure, Monica H Dholakia, Nicholas R Peterson, Ibolya Rutkai, Padmini S Mahalingam, Ryosuke Satou, Prasad V G Katakam
Mitochondria play a critical role in the cardiomyocyte physiology by generating majority of the ATP required for the contraction/relaxation through oxidative phosphorylation (OXPHOS). Aging is a major risk factor for cardiovascular diseases (CVD) and mitochondrial dysfunction has been proposed as potential cause of aging. Recent technological innovations in Seahorse XFe24 Analyzer enhanced the detection sensitivity of oxygen consumption rate and proton flux to advance our ability study mitochondrial function...
June 2, 2018: GeroScience
Hamid Niksirat, Christoph Steinbach
Detection of patterns of subcellular calcium distribution in the cardiovascular system can contribute to understanding its role in cardiac and blood function. The present study localized calcium in heart atrium, ventricle, and bulbus arteriosus as well as in erythrocytes of zebrafish Danio rerio using an oxalate-pyroantimonate technique combined with transmission electron microscopy. Intracellular calcium stores were detected in caveolae, mitochondria, and the nuclei of several zebrafish cardiac cell types...
May 24, 2018: Micron: the International Research and Review Journal for Microscopy
Jake Jianjun Wen, Yuhui Whitney Yin, Nisha Jain Garg
Chagasic cardiomyopathy is caused by Trypanosoma cruzi infection. Poly(ADP-ribose) polymerase 1 (PARP1) is known for its function in nuclear DNA repair. In this study, we have employed genetic deletion and chemical inhibition approaches to determine the role of PARP1 in maintaining mtDNA dependent mitochondrial function in Chagas disease. Our data show that expression of PARP1 and protein PARylation were increased by >2-fold and >16-fold, respectively, in the cytosolic, nuclear, and mitochondrial fractions of the human cardiac myocytes and the myocardium of wildtype (WT) mice chronically infected with T...
May 2018: PLoS Pathogens
Tomas A Schiffer, Håkan Gustafsson, Fredrik Palm
The kidneys receive approximately 25% of cardiac output, which is a prerequisite in order to maintain sufficient glomerular filtration rate. However, both intrarenal regional renal blood flow and tissue oxygen levels are heterogeneous with decreasing levels in the inner part of the medulla. These differences in combination with the heterogeneous metabolic activity of the different nephron segment located in the different parts of the kidney may constitute a functional problem when challenged. The proximal tubule and the medullary thick ascending limb of Henle are considered to have the highest metabolic rate, which is relating to the high mitochondria content needed to sustain sufficient ATP production from oxidative phosphorylation in order to support high electrolyte transport activity in these nephron segments...
May 30, 2018: American Journal of Physiology. Renal Physiology
Fang Li, Fangfang Lang, Yidan Wang, Chunxiao Zhai, Chuanbei Zhang, Liping Zhang, Enkui Hao
Cyanidin, an anthocyanin pigment, demonstrates anti-oxidant and anti-inflammatory properties. Here, we examined the mechanistic role of cyanidin in endotoxin induced myocardial injury in inflammation and oxidative stress. In lipopolysaccharide (LPS) induced myocardial injury model, cyanidin ameliorated cardiac injury (Lactate dehydrogenase or LDH, Creatine Kinase or CK, cardiac troponin I or cTnI and cardiac myosin light chains 1 or cMLC1), cell death (caspase 3 activity and PARP activity), and improved cardiac function (ejection fraction or EF and end diastolic left ventricular inner dimension or LVID)...
May 24, 2018: Food and Chemical Toxicology
Da Hye Kim, Shelly Chauhan
Cardiac metabolism depends on a high rate of ATP production to maintain its myocardial pump function. Under physiological conditions, more than 70% of ATP generated in the heart is derived from beta oxidation of fatty acids (FA) in the mitochondria. This article is protected by copyright. All rights reserved.
May 21, 2018: Journal of Physiology
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