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sparc, osteonectin

Maria Chatzinikolaidou, Charalampos Pontikoglou, Konstantina Terzaki, Maria Kaliva, Athanasia Kalyva, Eleni Papadaki, Maria Vamvakaki, Maria Farsari
The regeneration of bone via a tissue engineering approach involves components from the macroscopic to the nanoscopic level, including appropriate 3D scaffolds, cells and growth factors. In this study, hexagonal scaffolds of different diagonals were fabricated by Direct Laser Writing using a photopolymerizable hybrid material. The proliferation of bone marrow (BM) mesenchymal stem cells (MSCs) cultured on structures with various diagonals, 50, 100, 150 and 200μm increased significantly after 10days in culture, however without significant differences among them...
October 13, 2016: Colloids and Surfaces. B, Biointerfaces
Brett Nixon, Heath Ecroyd, Jean-Louis Dacheux, Francoise Dacheux, Valerie Labas, Steve D Johnston, Russell C Jones
As monotremes are the earliest offshoot of the mammalian lineage, the platypus and short-beaked echidna were studied as model animals to assess the origin and biological significance of adaptations considered unique to therian mammals: epididymal maturation and subsequent capacitation. We show that spermatozoa from both species assemble into bundles of approximately 100 cells during passage through the epididymis and that an epididymal protein, secreted protein, acidic, cysteine-rich (osteonectin) (SPARC), is involved in bundle formation...
August 24, 2016: Biology of Reproduction
Qi Chen, Yuan-Ting Yao, Huan Xu, Yan-Bo Chen, Meng Gu, Zhi-Kang Cai, Zhong Wang
Prostate cancer is the most diagnosed noncutaneous cancer and ranks as the second leading cause of cancer-related deaths in American males. Metastasis is the primary cause of prostate cancer mortality. Survival rate is only 28% for metastatic patients, but is nearly 100% for patients with localized prostate cancers. Molecular mechanisms that underlie this malignancy remain obscure, and this study investigated the role of SPARC/osteonectin, cwcv, and kazal-like domain proteoglycan 1 (SPOCK1) in prostate cancer progression...
2016: Drug Design, Development and Therapy
Anjie Zhu, Peng Yuan, Feng Du, Ruoxi Hong, Xiaoyan Ding, Xiuqing Shi, Ying Fan, Jiayu Wang, Yang Luo, Fei Ma, Pin Zhang, Qing Li, Binghe Xu
SPARC/osteonectin expression is reportedly altered in various malignancies. However, little is known regarding to the prognostic value of SPARC in triple-negative breast cancer (TNBC) patients. In this study, immunohistochemistry and immunoreactive scores (IRSs) were used to evaluate SPARC protein expression in primary tumors from 211 TNBC patients with up to 10 years of clinical follow-up data. High SPARC expression (IRS ≥3) was detected in 52.1% of primary tumors. Patients expressing high SPARC levels had worse disease-free survival (DFS) (HR=1...
July 11, 2016: Oncotarget
Paola Ciceri, Francesca Elli, Laura Cappelletti, Delfina Tosi, Federica Savi, Gaetano Bulfamante, Mario Cozzolino
Osteonectin, also termed SPARC, is a noncollagenous protein of bone matrix. Since there are controversial results regarding its role during the process of vascular calcification, we investigated osteonectin expression in our in vitro calcification model. Rat vascular smooth muscle cells (VSMCs) were challenged with high phosphate (5 mmol/L Pi) and analyzed quantifying calcium levels, through immunohistochemical studies, and studying gene expression. We detected a peak of osteonectin expression at day 7 in cell treated with high phosphate...
June 23, 2016: Calcified Tissue International
Estanislao Peixoto, Catalina Atorrasagasti, Mariana Malvicini, Esteban Fiore, Marcelo Rodriguez, Mariana Garcia, Paola Finocchieto, Juan J Poderoso, Fernando Corrales, Guillermo Mazzolini
SPARC, also known as osteonectin and BM-40, is a matricellular protein with a number of biological functions. Hepatic SPARC expression is induced in response to thioacetamide, bile-duct ligation, and acute injuries such as concanavalin A and lipopolysacharide (LPS)/D-galactosamine. We have previously demonstrated that the therapeutic inhibition of SPARC or SPARC gene deletion protected mice against liver injury. We investigated the mechanisms involved in the protective effect of SPARC inhibition in mice. We performed a proteome analysis of livers from SPARC+/+ and SPARC-/- mice chronically treated with thioacetamide...
May 18, 2016: Oncotarget
Jinghui Yang, Qiwei Yang, Jing Yu, Ximeng Li, Shan Yu, Xuewen Zhang
Sparc/osteonectin, cwcv and kazal-like domains proteoglycan (testican) 1 (SPOCK1) has been reported to promote the growth and progression of various tumors. In this study, we focus on assessing the effect of SPOCK1 on proliferation, migration and invasion in glioma cells and elucidating its related mechanisms. The results of our present study demonstrated that overexpression of SPOCK1 promoted the proliferation and inhibited apoptosis in glioma cells. Additionally, overexpression of SPOCK1 promoted the migration and invasion potential of glioma cells...
June 2016: Oncology Reports
Emma L Wilson, Mark Garton, Heidi R Fuller
Phenytoin is an antiepileptic drug used in the management of partial and tonic-clonic seizures. In previous studies we have shown that valproate, another antiepileptic drug, reduced the amount of two key bone proteins, pro-collagen I and osteonectin (SPARC, BM-40), in both skin fibroblasts and cultured osteoblast-like cells. Here we show that phenytoin also reduces pro-collagen I production in osteoblast-like cells, but does not appear to cause a decrease in osteonectin message or protein production. Instead, a 24h exposure to a clinically relevant concentration of phenytoin resulted in a dose-dependent change in electrophoretic mobility of osteonectin, which was suggestive of a change in post-translational modification status...
May 2016: Epilepsy Research
Monica K Rossi, Manu Gnanamony, Christopher S Gondi
Pancreatic ductal adenocarcinoma (PDAC) is one of the most clinically challenging cancers to manage. An estimated 48,960 people will be diagnosed with pancreatic cancer in 2015, of that population, 94% are projected to perish within 5 years. These dismal survival rates can be attributed, in part, to an advanced diagnosis occurring in 80% of cases. The heterogeneous and dynamic microenvironment of pancreatic cancer, and the lack of both specific risk factors and efficacious screening tools contribute to the challenge of diagnosing pancreatic cancer in its early stages...
May 2016: International Journal of Oncology
Yingying Hong, Jianyun Zhang, Heyu Zhang, Xuefen Li, Jiafei Qu, Jiemei Zhai, Lei Zhang, Feng Chen, Tiejun Li
Nevoid basal cell carcinoma syndrome (NBCCS) is an autosomal dominant disorder characterized by bone and skin abnormalities and a predisposition to various tumors. Keratocystic odontogenic tumors (KCOTs), which are common tumors of the jaw that cause extensive damage to the jawbone, are usually accompanied with NBCCS. Germline PTCH1 mutations in NBCCS tumorigenesis have been frequently studied; however, little is known regarding the pathogenesis of bone abnormalities in this disease. This study sought to investigate the mechanism underlying heterozygous PTCH1 mutation-mediated abnormal bone metabolism in patients with NBCCS...
July 2016: Journal of Bone and Mineral Research: the Official Journal of the American Society for Bone and Mineral Research
Emilie M Rosset, Amy D Bradshaw
Secreted protein acidic and rich in cysteine (SPARC/osteonectin/BM40) is one of the most abundant non-collagenous protein expressed in mineralized tissues. This review will focus on elucidating functional roles of SPARC in bone formation building upon results from non-mineralized cells and tissues, the phenotype of SPARC-null bones, and recent discoveries of human diseases with either dysregulated expression of SPARC or mutations in the gene encoding SPARC that give rise to bone pathologies. The capacity of SPARC to influence pathways involved in extracellular matrix assembly such as procollagen processing and collagen fibril formation as well as the capacity to influence osteoblast differentiation and osteoclast activity will be addressed...
May 2016: Matrix Biology: Journal of the International Society for Matrix Biology
E Płuciennik, M Nowakowska, M Gałdyszyńska, M Popęda, A K Bednarek
The purpose of the present study was to investigate the role of WW domain containing oxidoreductase (WWOX) downregulation in biological cancer-related processes in normal (non-malignant) and cancer endometrial cell lines. We created an in vitro model using the normal endometrial cell line, THESC, and 2 endometrial cancer cell lines with varying degrees of differentiation, the Ishikawa (well-differentiated) and the MFE296 (moderately differentiated) cells, in which the WWOX tumor suppressor gene was silenced using Gipz lentiviral shRNA...
March 2016: International Journal of Molecular Medicine
Yu-Ching Chen, Sarah Statt, Reen Wu, Hao-Teng Chang, Jiunn-Wang Liao, Chien-Neng Wang, Woei-Cherng Shyu, Chen-Chen Lee
Epithelial-mesenchymal transition (EMT) is implicated in bronchial remodeling and loss of lung function in chronic inflammatory airway diseases. Previous studies showed the involvement of the high mobility group box 1 (HMGB1) protein in the pathology of chronic pulmonary inflammatory diseases. However, the role of HMGB1 in EMT of human airway epithelial cells is still unclear. In this study, we used RNA sequencing to show that HMGB1 treatment regulated EMT-related gene expression in human primary-airway epithelial cells...
2016: Scientific Reports
Neha S Dole, Anne M Delany
Single nucleotide polymorphisms (SNPs) are the most abundant genetic variants that contribute to the heritability of bone mass. MicroRNAs (miRNAs, miRs) are key post-transcriptional regulators that modulate the differentiation and function of skeletal cells by targeting multiple genes in the same or distinct signaling pathways. SNPs in miRNA genes and miRNA binding sites can alter miRNA abundance and mRNA targeting. This review describes the potential impact of miRNA-related SNPs on skeletal phenotype. Although many associations between SNPs and bone mass have been described, this review is limited to gene variants for which a function has been experimentally validated...
March 2016: Bone
Antonia P Popova, Tracy X Cui, Niko Kaciroti, Adam M Goldsmith, Marisa J Linn, Gloria S Pryhuber, Marc B Hershenson
BACKGROUND: Isolation of tracheal aspirate mesenchymal stromal cells (MSCs) from premature infants has been associated with increased risk of bronchopulmonary dysplasia (BPD). MSCs show high levels of mRNAs encoding matricellular proteins, non-structural extracellular proteins that regulate cell-matrix interactions and participate in tissue remodeling. We hypothesized that lung matricellular protein expression predicts BPD development. METHODS: We collected tracheal aspirates and MSCs from mechanically-ventilated premature infants during the first week of life...
2015: PloS One
Michel Goldberg, Akram Njeh, Emel Uzunoglu
The importance of inflammation has been underestimated in pulpal healing, and in the past, it has been considered only as an undesirable effect. Associated with moderate inflammation, necrosis includes pyroptosis, apoptosis, and nemosis. There are now evidences that inflammation is a prerequisite for pulp healing, with series of events ahead of regeneration. Immunocompetent cells are recruited in the apical part. They slide along the root and migrate toward the crown. Due to the high alkalinity of the capping agent, pulp cells display mild inflammation, proliferate, and increase in number and size and initiate mineralization...
2015: Mediators of Inflammation
Emanuela Matteucci, Paola Maroni, Andrea Disanza, Paola Bendinelli, Maria Alfonsina Desiderio
The pathogenesis of bone metastasis is unclear, and much focus in metastatic biology and therapy relays on epigenetic alterations. Since DNA-methyltransferase blockade with 5-aza-2'-deoxycytidine (dAza) counteracts tumour growth, here we utilized dAza to clarify whether molecular events undergoing epigenetic control were critical for bone metastatization. In particular, we investigated the patterns of secreted-protein acidic and rich in cysteine (SPARC) and of Endothelin 1, affected by DNA methyltransferases in tumours, with the hypothesis that in bone metastasis a coordinate function of SPARC and Endothelin 1, if any occurs, was orchestrated by DNA methylation...
January 2016: Biochimica et Biophysica Acta
Francisco J López-Murcia, Beatrice Terni, Artur Llobet
Elimination of the excess synaptic contacts established in the early stages of neuronal development is required to refine the function of neuronal circuits. Here we investigate whether secreted protein acidic and rich in cysteine (SPARC), a molecule produced by glial cells, is involved in synapse removal. SPARC production peaks when innervation of the rat superior cervical ganglion and the tail of Xenopus tropicalis tadpoles are remodeled. The formation of new cholinergic synapses in autaptic single-cell microcultures is inhibited by SPARC...
October 27, 2015: Proceedings of the National Academy of Sciences of the United States of America
Adam J Isabella, Sally Horne-Badovinac
Basement membranes (BMs) are sheet-like extracellular matrices that provide essential support to epithelial tissues. Recent evidence suggests that regulated changes in BM architecture can direct tissue morphogenesis, but the mechanisms by which cells remodel BMs are largely unknown. The Drosophila egg chamber is an organ-like structure that transforms from a spherical to an ellipsoidal shape as it matures. This elongation coincides with a stage-specific increase in Type IV Collagen (Col IV) levels in the BM surrounding the egg chamber; however, the mechanisms and morphogenetic relevance of this remodeling event have not been established...
October 15, 2015: Developmental Biology
Luca Dalle Carbonare, Alessandro Matte', Maria Teresa Valenti, Angela Siciliano, Antonio Mori, Vittorio Schweiger, Gino Zampieri, Luigi Perbellini, Lucia De Franceschi
Sickle cell disease (SCD) is a worldwide distributed hereditary red cell disorder, characterized by severe organ complication. Sickle bone disease (SBD) affects a large part of the SCD patient population, and its pathogenesis has been only partially investigated. Here, we studied bone homeostasis in a humanized mouse model for SCD. Under normoxia, SCD mice display bone loss and bone impairment, with increased osteoclast and reduced osteoblast activity. Hypoxia/reperfusion (H/R) stress, mimicking acute vaso-occlusive crises (VOCs), increased bone turnover, osteoclast activity (RankL), and osteoclast recruitment (Rank) with upregulation of IL-6 as proresorptive cytokine...
November 12, 2015: Blood
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