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https://www.readbyqxmd.com/read/28289857/-neuronal-like-differentiation-of-murine-mesenchymal-stem-cell-line-stimulation-by-juglans-regia-l-oil
#1
Varsha Singh, Mingma Sherpa
Mesenchymal stem cells have been extensively used for cell-based therapies especially in neuronal diseases. Studies still continue to delineate mechanisms involved in differentiating mesenchymal stem cells into neuronal cells under experimental conditions as they have low mortality rate and hence, the number of cells available for experiments is much more limited. Culturing and differentiating of neuronal cell is more challenging as they do not undergo cell division thus, bringing them to differentiate proves to be a difficult task...
March 13, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/28286541/an-evaluation-of-inexpensive-methods-for-root-image-acquisition-when-using-rhizotrons
#2
Awaz Mohamed, Yogan Monnier, Zhun Mao, Guillaume Lobet, Jean-Luc Maeght, Merlin Ramel, Alexia Stokes
BACKGROUND: Belowground processes play an essential role in ecosystem nutrient cycling and the global carbon budget cycle. Quantifying fine root growth is crucial to the understanding of ecosystem structure and function and in predicting how ecosystems respond to climate variability. A better understanding of root system growth is necessary, but choosing the best method of observation is complex, especially in the natural soil environment. Here, we compare five methods of root image acquisition using inexpensive technology that is currently available on the market: flatbed scanner, handheld scanner, manual tracing, a smartphone application scanner and a time-lapse camera...
2017: Plant Methods
https://www.readbyqxmd.com/read/28271176/classification-and-ordination-of-understory-vegetation-using-multivariate-techniques-in-the-pinus-wallichiana-forests-of-swat-valley-northern-pakistan
#3
Inayat Ur Rahman, Nasrullah Khan, Kishwar Ali
An understory vegetation survey of the Pinus wallichiana-dominated temperate forests of Swat District was carried out to inspect the structure, composition and ecological associations of the forest vegetation. A quadrat method of sampling was used to record the floristic and phytosociological data necessary for the analysis using 300 quadrats of 10 × 10 m each. Some vegetation parameters viz. frequency and density for trees (overstory vegetation) as well as for the understory vegetation were recorded. The results revealed that in total, 92 species belonging to 77 different genera and 45 families existed in the area...
April 2017: Die Naturwissenschaften
https://www.readbyqxmd.com/read/28257470/rethinking-the-history-of-common-walnut-juglans-regia-l-in-europe-its-origins-and-human-interactions
#4
Paola Pollegioni, Keith Woeste, Francesca Chiocchini, Stefano Del Lungo, Marco Ciolfi, Irene Olimpieri, Virginia Tortolano, Jo Clark, Gabriel E Hemery, Sergio Mapelli, Maria Emilia Malvolti
Common walnut (Juglans regia L) is an economically important species cultivated worldwide for its high-quality wood and nuts. It is generally accepted that after the last glaciation J. regia survived and grew in almost completely isolated stands in Asia, and that ancient humans dispersed walnuts across Asia and into new habitats via trade and cultural expansion. The history of walnut in Europe is a matter of debate, however. In this study, we estimated the genetic diversity and structure of 91 Eurasian walnut populations using 14 neutral microsatellites...
2017: PloS One
https://www.readbyqxmd.com/read/28243724/in-vitro-gene-expression-and-mrna-translocation-from-transformed-walnut-juglans-regia-rootstocks-expressing-dsred-fluorescent-protein-to-wild-type-scions
#5
Xiaochen Liu, Sriema L Walawage, Charles A Leslie, Abhaya M Dandekar, David M Tricoli, Hengkang Hu, Youjun Huang, Jiaqi Zhang, Chuanmei Xv, Jianqin Huang, Qixiang Zhang
An in vitro grafting method was developed for examining gene translocation from rootstock to scion in walnut. Results showed the DsRED gene itself was not translocated but expressed mRNA was. Grafting is widely used in plants, especially in fruit and nut crops. Selected rootstocks can control scion growth and physiological traits, including shortening of the juvenile phase and controlling tree size. Rootstocks also can provide improved soil adaptation and pathogen resistance. Development of genetically modified (GM) fruit crops has progressed recently, but commercial cultivation is still limited due to the time required for evaluation and issues with deregulation...
February 27, 2017: Plant Cell Reports
https://www.readbyqxmd.com/read/28191338/accelerated-burn-wound-closure-in-mice-with-a-new-formula-based-on-traditional-medicine
#6
Mehrnaz Mehrabani, Seyyed Mohsen Seyyedkazemi, Mohammad Hadi Nematollahi, Elham Jafari, Mitra Mehrabani, Mohammad Mehdipour, Zahra Sheikhshoaee, Ali Mandegary
BACKGROUND: A combination of the oils of sesame, hemp, wild pistachio, and walnut has been used for treatment of skin disorders, including wound burns, in some parts of Kerman, Iran. Evaluation of this remedy in the form of a pharmaceutical formulation in animal models can pave the way for its future application in wound burn healing in humans. OBJECTIVES: This experimental study investigated the healing potential of a new formula (NF) based on folk medicine from Iran for the treatment of third degree burns in mice...
November 2016: Iranian Red Crescent Medical Journal
https://www.readbyqxmd.com/read/28185293/assessing-frost-damages-using-dynamic-models-in-walnut-trees-exposure-rather-than-vulnerability-controls-frost-risks
#7
Guillaume Charrier, Isabelle Chuine, Marc Bonhomme, Thierry Améglio
Frost damages develop when exposure overtakes frost vulnerability. Frost risk assessment therefore needs dynamic simulation of frost hardiness using temperature and photoperiod in interaction with developmental stage. Two models, including or not the effect of photoperiod were calibrated using five years of frost hardiness monitoring (2007-2012), in two locations (low and high elevation) for three walnut genotypes with contrasted phenology and maximum hardiness (Juglans regia cv Franquette, Juglans regia x nigra 'Early' and 'Late')...
February 9, 2017: Plant, Cell & Environment
https://www.readbyqxmd.com/read/28132043/the-effect-of-extract-of-greek-walnut-juglans-regia-l-septa-on-some-functional-characteristics-of-erythrocytes
#8
L Ramishvili, M Gordeziani, E Tavdishvili, N Bedineishvili, D Dzidziguri, N Kotrikadze
Administration of plant extracts for the treatment of several different diseases is an important approach ofmodern medicine.The reason must be an easy way of application, low price and the complex action of herbal medicines. The aim of the work was to study the effect of extract of walnut (Juglans regia L.) septa on the functional characteristics of erythrocytes during administration of cytotoxic agent - cyclophosphamide (experimental model of leukopenia). The material for the study was blood of the intact and experimental white mice...
December 2016: Georgian Medical News
https://www.readbyqxmd.com/read/28101461/acute-and-subchronic-toxicity-study-of-the-median-septum-of-juglans-regia-in-wistar-rats
#9
Asma Ravanbakhsh, Majid Mahdavi, Ghader Jalilzade-Amin, Shahram Javadi, Masoud Maham, Daryosh Mohammadnejad, Mohammad Reza Rashidi
Purpose: Median septum of Juglans regia L. (Juglandaceae) with anti-diabetic effects has been used in Iranian traditional medicine. The present study estimates both oral acute and subchronic toxicities. Methods: In the oral acute toxicity study, female Wistar rats were treated with doses of 10, 100, 1000, 1600, 2900 and 5000 mg/ kg of the Juglans regia septum of methanol extract (JRSME), and were monitored for 14 days. In subchronic study, JRSME was administered by gavage at dose of 1000 mg/kg daily in Wistar rats for 28 days...
December 2016: Advanced Pharmaceutical Bulletin
https://www.readbyqxmd.com/read/28070926/identification-and-implication-of-an-allergenic-pr-10-protein-from-walnut-in-birch-pollen-associated-walnut-allergy
#10
Andrea Wangorsch, Annette Jamin, Jonas Lidholm, Nora Gräni, Claudia Lang, Barbara Ballmer-Weber, Stefan Vieths, Stephan Scheurer
SCOPE: English walnut (Juglans regia) belongs to the most important allergenic tree nuts. Co-sensitization with birch (Betula verrucosa) pollen has been reported. We aimed to identify a walnut allergen homologous to the major birch pollen allergen Bet v 1. METHODS AND RESULTS: A cDNA encoding a Bet v 1-homologous allergen (Jug r 5) in walnut kernels was cloned by RT-PCR. Jug r 5 was expressed in E.coli, purified by column chromatography and characterized by CD-spectroscopy...
January 9, 2017: Molecular Nutrition & Food Research
https://www.readbyqxmd.com/read/28004034/klebsiella-pneumoniae-planktonic-and-biofilm-reduction-by-different-plant-extracts-in-vitro-study
#11
Lucas De Paula Ramos, Carlos Eduardo da Rocha Santos, Daphne Camargo Reis Mello, Lígia Nishiama Theodoro, Felipe Eduardo De Oliveira, Graziella N Back Brito, Juliana Campos Junqueira, Antonio Olavo Cardoso Jorge, Luciane Dias de Oliveira
This study evaluated the action of Pfaffia paniculata K., Juglans regia L., and Rosmarius officinalis L. extracts against planktonic form and biofilm of Klebsiella pneumoniae (ATCC 4352). Minimum inhibitory concentration (MIC) and minimum microbicidal concentration (MMC) values were determined for each extract by microdilution broth method, according to Clinical and Laboratory Standards Institute. Next, antimicrobial activity of the extracts on biofilm was analyzed. For this, standardized suspension at 10(7) UFC/mL of K...
2016: TheScientificWorldJournal
https://www.readbyqxmd.com/read/28000512/developmental-role-of-phenylalanine-ammonia-lyase-pal-and-cinnamate-4-hydroxylase-c4h-genes-during-adventitious-rooting-of-juglans-regia-l-microshoots
#12
Monireh Cheniany, Ali Ganjeali
Phenylalanine-ammonia-lyase and cinnamate-4-hydroxylase play important role in the phenylpropanoid pathway, which produces many biologically important secondary metabolites participating in normal plant development. Flavonol quercetin is the main representant of these compounds that has been identified in numerous Juglans spp. In this survey, the developmental expression patterns of PAL and C4H genes during in vitro rooting of two walnut cultivars 'Sunland' and 'Howard' was examined by RT-PCR. To understand the potential role in rooting, the changing pattern of endogenous content of quercetin was also analyzed by HPLC...
December 2016: Acta Biologica Hungarica
https://www.readbyqxmd.com/read/27986993/characterization-of-a-vacuolar-h-atpase-g-subunit-gene-from-juglans-regia-jrvhag1-involved-in-mannitol-induced-osmotic-stress-tolerance
#13
Zhenggang Xu, Yunlin Zhao, Yu Ge, Jiao Peng, Meng Dong, Guiyan Yang
JrVHAG1 is an important candidate gene for plant osmotic tolerance regulation. Vacuolar H(+)-ATPase (V-ATPase) is important for plant responses to abiotic stress; the G subunit is a vital part of V-ATPase. In this study, a G subunit of V-ATPase was cloned from Juglans regia (JrVHAG1) and functionally characterized. JrVHAG1 transcription was induced by mannitol that increasing 17.88-fold in the root at 12 h and 19.16-fold in the leaf at 96 h compared to that under control conditions. JrVHAG1 was overexpressed in Arabidopsis and three lines (G2, G6, and G9) with highest expression levels were selected for analysis...
March 2017: Plant Cell Reports
https://www.readbyqxmd.com/read/27860167/both-jrwrky2-and-jrwrky7-of-juglans-regia-mediate-responses-to-abiotic-stresses-and-abscisic-acid-through-formation-of-homodimers-and-interaction
#14
G Yang, W Zhang, Z Liu, A-Y Yi-Maer, M Zhai, Z Xu
WRKY transcription factors belong to a large protein family that is involved in diverse developmental processes and abiotic stress responses. Currently, there is little understanding of the role of WRKY transcription factors in regulatory mechanisms in plants, especially in the protein-protein interactions that are essential for biological regulatory functions and networks. In the present study, yeast one-hybrid, yeast two-hybrid, transient expression and quantitative RT-PCR were applied to investigate the potential characteristics of two WRKY proteins from Juglans regia, JrWRKY2 (GenBank Accession No...
March 2017: Plant Biology
https://www.readbyqxmd.com/read/27852104/a-pair-of-taxifolin-3-o-arabinofuranoside-isomers-from-juglans-regia-l
#15
Huanxin Zhao, Hong Bai, Yuanchao Jing, Wei Li, Shujun Yin, Honglei Zhou
Diaphragma juglandis Fructus has been known for its effect on tonifying kidney in traditional Uighur medicine. During our ongoing studies on the discovery of biologically active compounds from folk medicine, a pair of flavanonol glycoside isomers named (2S,3S)-taxifolin-3-O-α-d-arabinofuranoside (1) and (2S,3S)-taxifolin-3-O-α-l-arabinofuranoside (2) were isolated, among them, 1 was a new compound. It is the first report of taxifolin-3-O-arabinofuranosides in both α-d and α-l configuration forms in genus Juglans...
April 2017: Natural Product Research
https://www.readbyqxmd.com/read/27794507/a-novel-survey-of-the-ethno-medicinal-knowledge-of-dental-problems-in-manoor-valley-northern-himalaya-pakistan
#16
Inayat Ur Rahman, Farhana Ijaz, Zafar Iqbal, Aftab Afzal, Niaz Ali, Muhammad Afzal, Muhammad Azhar Khan, Said Muhammad, Ghulam Qadir, Muhammad Asif
ETHNOPHARMACOLOGICAL RELEVANCE: In the study area, the traditional knowledge and cultural practices of utilizing medicinal plants in the human healthcare is primarily restricted to the old age members of the community, while younger generation is mostly unaware of the natural wealth usage. AIM OF THE STUDY: We provide the first ever ethno medicinal insights into the management of dental disorders in Pakistan. The prime objective of the study was to explore novel knowledge of these local and remote community members and share it with rest of the world in documented form...
December 24, 2016: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/27744101/comparison-of-antifungal-activity-of-extracts-from-different-juglans-regia-cultivars-and-juglone
#17
D Wianowska, S Garbaczewska, A Cieniecka-Roslonkiewicz, A L Dawidowicz, A Jankowska
This study discusses the similarities and differences between the antifungal activity of extracts from walnut green husks of Lake, Koszycki, UO1, UO2 and non-grafted cultivars as well as juglone against the plant pathogenic fungi such as Alternaria alternata, Rhizoctonia solani, Botrytis cinerea, Fusarium culmorum, Phytophthora infestans as well as Ascosphaera apis causing chalkbrood disease in honey bees. The obtained data show that the antifungal activities of the extracts do not always depend on the antifungal activity of juglone, and that they can be modulated by their other components...
November 2016: Microbial Pathogenesis
https://www.readbyqxmd.com/read/27734599/cultivated-walnut-trees-showed-earlier-but-not-final-advantage-over-its-wild-relatives-in-competing-for-seed-dispersers
#18
COMPARATIVE STUDY
Hongmao Zhang, Wei Chu, Zhibin Zhang
Little is known about seeding regeneration of cultivated trees compared to wild relatives in areas where seed dispersers are shared. Here, we investigated the differences in seed fates of cultivated walnut (Juglans regia) and wild Manchurian walnut (Juglans mandshurica) trees under rodent predation and dispersal. J. regia seeds have higher nutritional value (large size, mass and kernel mass) and lower mechanical defensiveness (thin endocarp) than J. mandshurica seeds. We tracked seeds of J. regia and J. mandshurica under both enclosure and field conditions to assess differences in competing for seed dispersers of the two co-occurring tree species of the same genus...
January 2017: Integrative Zoology
https://www.readbyqxmd.com/read/27717454/short-versus-long-term-effects-of-cyanide-on-sugar-metabolism-and-transport-in-dormant-walnut-kernels
#19
Zahra Gerivani, Elham Vashaee, Hamid Reza Sadeghipour, Mahnaz Aghdasi, Zahra-Sadat Shobbar, Majid Azimmohseni
Tree seed dormancy release by cold stratification accompanies with the embryo increased gluconeogenesis competence. Cyanide also breaks seed dormancy however, integrated information about its effects on carbon metabolism is lacking. Accordingly, the impacts of HCN on germination, lipid gluconeogenesis and sugar transport capacity of walnut (Juglans regia L.) kernels were investigated during 10-days period prior to radicle protrusion. HCN increased walnut kernel germination and within four days of kernel incubation, hastened the decline of starch, reducing and non-reducing sugars and led to greater activities of alkaline invertase and glucose-6-phosphate dehydrogenase...
November 2016: Plant Science: An International Journal of Experimental Plant Biology
https://www.readbyqxmd.com/read/27679440/antioxidant-and-anticancer-activities-of-walnut-juglans-regia-l-protein-hydrolysates-using-different-proteases
#20
Raheleh Jahanbani, S Mahmood Ghaffari, Maryam Salami, Kourosh Vahdati, Houri Sepehri, Nazanin Namazi Sarvestani, Nader Sheibani, Ali Akbar Moosavi-Movahedi
Walnut (Juglans regia L.) contains approximately 20-25 % protein with abundant essential amino acids. The enzymatic hydrolysate of Persian walnut (Chandler) seed proteins was prepared by incubation with three different proteases, including pancreatic chymotrypsin and trypsin, and a microbial enzyme proteinase K. The hydrolysates were found to possess excellent antioxidant capacities. The peptide fractions scavenged the 2, 2'-anizo-bis-(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) free radicals and inhibited the activity of reactive oxygen species...
December 2016: Plant Foods for Human Nutrition
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