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https://www.readbyqxmd.com/read/29668464/effects-of-%C3%AE-prazosin-and-yohimbine-and-%C3%AE-receptors-activity-on-camp-generation-and-ucp1-gene-expression-in-brown-adipocytes
#1
Hirendra M Biswas
BACKGROUND: Brown adipose tissue (BAT) contains both α- and β-adrenergic receptors. In the literature, the activity of α-adrenoreceptors is less documented, and their functions still remain puzzling. The present investigation has been undertaken to understand α-adrenoreceptors' activity and their relation between uncoupling protein 1 (UCP1) mRNA expression and cyclic AMP (cAMP) generation in BAT. METHODS: BAT precursor cells from young mice were grown in culture...
April 18, 2018: Journal of Basic and Clinical Physiology and Pharmacology
https://www.readbyqxmd.com/read/29644261/detection-and-analysis-of-circular-rnas-by-rt-pcr
#2
Amaresh C Panda, Myriam Gorospe
Gene expression in eukaryotic cells is tightly regulated at the transcriptional and posttranscriptional levels. Posttranscriptional processes, including pre-mRNA splicing, mRNA export, mRNA turnover, and mRNA translation, are controlled by RNA-binding proteins (RBPs) and noncoding (nc)RNAs. The vast family of ncRNAs comprises diverse regulatory RNAs, such as microRNAs and long noncoding (lnc)RNAs, but also the poorly explored class of circular (circ)RNAs. Although first discovered more than three decades ago by electron microscopy, only the advent of high-throughput RNA-sequencing (RNA-seq) and the development of innovative bioinformatic pipelines have begun to allow the systematic identification of circRNAs (Szabo and Salzman, 2016; Panda et al ...
March 20, 2018: Bio-protocol
https://www.readbyqxmd.com/read/29593895/cross-sectional-serological-survey-of-human-fascioliasis-in-canutama-municipality-in-western-amazon-brazil
#3
Marcel Gonçalves Maciel, Walter Dos Santos Lima, Francisco Lazaro Moreira de Almeida, Leila Inês Aguiar Raposo Câmara Coelho, Guilherme Alfredo Novelino Araújo, Mariana Gomes Lima, Luiz Henrique Gonçalves Maciel, Cíntia Aparecida de Jesus Pereira, Thaís Costa da Silva Maciel, Jorge Augusto de Oliveira Guerra, Rosa Amélia Gonçalves Santana, Maria das Graças Vale Barbosa Guerra
Background: Fascioliasis is an important parasitic disease. In the northern region of Brazil, a human parasite infection has been reported through a coprological survey. Eggs of Fasciola hepatica were found in fecal samples of 11 individuals. Knowledge of the infection in animals or the presence of snails is necessary to address the possibility of the parasite cycle occurrence in that region. The aim of this study was to describe the transmission of human fascioliasis in Canutama, Amazonas, in Western Amazonia, Brazil...
2018: Journal of Parasitology Research
https://www.readbyqxmd.com/read/29588481/comparative-genome-wide-analysis-of-extracellular-small-rnas-from-the-mucormycosis-pathogen-rhizopus-delemar
#4
Muxing Liu, Gillian O Bruni, Christopher M Taylor, Zhengguang Zhang, Ping Wang
Rhizopus delemar is an emerging fungal pathogen causing devastating mucormycosis in immunocompromised individuals. The organism remains understudied and there are urgent needs for new methods of rapid disease diagnosis for timely therapy. Extracellular vesicles with encapsulated RNAs have recently been discovered to have great potential applications for disease diagnoses and treatments. To explore the utilization of ex-RNA in studies of mucormycosis, we have performed RNA-Seq of ex-sRNAs from two clinical strains of R...
March 27, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29574975/lncrna-flvcr1-as1-acts-as-mir-513c-sponge-to-modulate-cancer-cell-proliferation-migration-and-invasion-in-hepatocellular-carcinoma
#5
Kunsong Zhang, Zhenxian Zhao, Junfeng Yu, Wei Chen, Qiongcong Xu, Liuhua Chen
In the present study, we aimed to search for dysregulated lnRNAs in Hepatocellular carcinoma (HCC) tissues, and analyze the relationship of its expression level with the clinicopathological feature and patient prognosis. The biological function of FLVCR1-AS1, the identified lncRNA, in the process of HCC development, and progression was investigated in vitro and in vivo. The underlying molecular mechanism was further explored. We determined FLVCR1-AS1 expression in HCC tissues and peri-tumor tissues by bioinformatic analysis, qRT-PCR, Northern blot and in situ hybridization...
March 25, 2018: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/29554924/molecular-evidence-for-the-transovarial-passage-of-babesia-gibsoni-in-haemaphysalis-hystricis-acari-ixodidae-ticks-from-taiwan-a-novel-vector-for-canine-babesiosis
#6
Frans Jongejan, Bi-Ling Su, Hsiang-Ju Yang, Laura Berger, Judith Bevers, Pin-Chen Liu, Jou-Chien Fang, Ya-Wen Cheng, Charlotte Kraakman, Nadine Plaxton
BACKGROUND: Babesia gibsoni is the predominant tick-borne protozoan blood parasite affecting dogs throughout the Oriental region. Babesia gibsoni is transmitted by Haemaphysalis longicornis, whereas a similar role has been suggested for Rhipicephalus sanguineus. Haemaphysalis longicornis does not occur in Taiwan, but R. sanguineus is widely distributed on dogs. However, clinical cases of babesiosis are mainly restricted to the northern part of the island. The discrepancy between tick distribution and clinical cases stimulated us to investigate the tick species distribution on dogs in northern Taiwan, with the aim to identify the local vector for canine babesiosis...
March 20, 2018: Parasites & Vectors
https://www.readbyqxmd.com/read/29550499/serodiagnosis-of-borrelia-miyamotoi-disease-by-measuring-antibodies-against-glpq-and-variable-major-proteins
#7
Joris Koetsveld, Nadezhda M Kolyasnikova, Alex Wagemakers, Olga A Stukolova, Dieuwertje Hoornstra, Denis S Sarksyan, Marina G Toporkova, Anna J Henningsson, Dag Hvidsten, Wim Ang, Ram Dessau, Alexander E Platonov, Joppe W Hovius
OBJECTIVES: Borrelia miyamotoi disease (BMD) is an emerging tick-borne disease in the Northern hemisphere. Serodiagnosis by measuring antibodies against glycerophosphodiester-phosphodiesterase (GlpQ) is performed experimentally, but has not been extensively clinically validated. Since we have previously shown the differential expression of antigenic variable major proteins (Vmps) in B. miyamotoi, our aim was to study antibody responses against GlpQ and Vmps in PCR-proven BMD patients and controls...
March 14, 2018: Clinical Microbiology and Infection
https://www.readbyqxmd.com/read/29542020/hsp60-expression-profile-under-different-extreme-temperature-stress-in-albino-northern-snakehead-channa-argus
#8
Aiguo Zhou, Shaolin Xie, Zhenlu Wang, Yanfeng Chen, Yue Zhang, Lanfen Fan, Fang Zeng, Jixing Zou
The great albino northern snakehead, Channa argus, is one of the most important economical fish in China. In the present study, cDNA encoding heat shock protein 60 (HSP60) was cloned and characterized. The cDNA was 2462 bp, containing an open reading frame (ORF) encoding a 575-amino-acids polypeptide of 61.10 kDa (theoretical isoelectric point [pI]: 5.66). BLAST analysis showed that AcaHSP60 was highly similar with other HSP60s, and three conserved amino acid blocks and characteristic motifs or domains defined as HSP60 protein family signatures...
March 14, 2018: Cell Stress & Chaperones
https://www.readbyqxmd.com/read/29527194/a-glycine-riboswitch-in-streptococcus-pyogenes-controls-expression-of-a-sodium-alanine-symporter-family-protein-gene
#9
Afsaneh Khani, Nicole Popp, Bernd Kreikemeyer, Nadja Patenge
Regulatory RNAs play important roles in the control of bacterial gene expression. In this study, we investigated gene expression regulation by a putative glycine riboswitch located in the 5'-untranslated region of a sodium:alanine symporter family (SAF) protein gene in the group A Streptococcus pyogenes serotype M49 strain 591. Glycine-dependent gene expression mediated by riboswitch activity was studied using a luciferase reporter gene system. Maximal reporter gene expression was observed in the absence of glycine and in the presence of low glycine concentrations...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29513794/the-hepatic-ectonucleotide-pyrophosphatase-phosphodiesterase-1-gene-mrna-abundance-is-reduced-by-insulin-and-induced-by-dexamethasone
#10
Huiwen Ma, Ping Wang, Dan Jin, Ting Jia, Hong Mao, Jiandi Zhang, Shi Zhao
Hormones regulate hepatic gene expressions to maintain metabolic homeostasis. Ectonucleotide pyrophosphatase/phosphodiesterase 1 has been thought to interfere with insulin signaling. To determine its potential role in the regulation of metabolism, we analyzed its gene (Enpp1) expression in the liver of rats experiencing fasting and refeeding cycles, and in primary rat hepatocytes and human hepatoma HepG2 cells treated with insulin and dexamethasone using northern blot and real-time PCR techniques. Hepatic Enpp1 expression was induced by fasting and reduced by refeeding in the rat liver...
March 1, 2018: Brazilian Journal of Medical and Biological Research, Revista Brasileira de Pesquisas Médicas e Biológicas
https://www.readbyqxmd.com/read/29490613/identification-and-characterization-of-micrornas-involved-in-ascidian-larval-metamorphosis
#11
Xiaoming Zhang, Xiaozhuo Liu, Chengzhang Liu, Jiankai Wei, Haiyan Yu, Bo Dong
BACKGROUND: Metamorphosis takes place within the life cycle of most marine invertebrates. The marine ascidian is a classical model to study complex cellular processes and underlying molecular mechanisms involved in its larval metamorphosis. The detailed molecular signaling pathways remain elusive, though extracellular signal-regulated kinases (ERKs) and c-Jun N-terminal kinase (JNK) have been revealed to regulate cell migration, differentiation, and apoptosis in ascidian larval organ regression and juvenile organ development...
March 1, 2018: BMC Genomics
https://www.readbyqxmd.com/read/29483058/occurrence-of-tick-borne-haemoparasites-in-cattle-in-the-mungwi-district-northern-province-zambia
#12
Stephen Tembo, Nicola E Collins, Kgomotso P Sibeko-Matjila, Milana Troskie, Ilse Vorster, Charles Byaruhanga, Marinda C Oosthuizen
Little is known about the occurrence of haemoparasites in cattle in communal grazing areas of Mungwi District of Northern Province, Zambia. Clinical signs and post mortem lesions are pathognomonic of mixed tick-borne infections especially babesiosis, anaplasmosis and East Coast fever. The main objective of this study was to screen selected communal herds of cattle for tick-borne haemoparasites, and identify the tick vectors associated with the high cattle mortalities due to suspected tick-borne diseases in the local breeds of cattle grazing along the banks of the Chambeshi River in Mungwi District, Northern Province, Zambia...
February 16, 2018: Ticks and Tick-borne Diseases
https://www.readbyqxmd.com/read/29482189/downregulation-of-the-cl-hco3-exchanger-pendrin-in-kidneys-of-mice-with-cystic-fibrosis-role-in-the-pathogenesis-of-metabolic-alkalosis
#13
Mujan Varasteh Kia, Sharon Barone, Alicia A McDonough, Kamyar Zahedi, Jie Xu, Manoocher Soleimani
BACKGROUND/AIMS: Patients with cystic fibrosis (CF) are prone to the development of metabolic alkalosis; however, the pathogenesis of this life threatening derangement remains unknown. We hypothesized that altered acid base transport machinery in the kidney collecting duct underlies the mechanism of impaired bicarbonate elimination in the CF kidney. METHODS: Balance studies in metabolic cages were performed in WT and CFTR knockout (CF) mice with the intestinal rescue in response to bicarbonate loading or salt restriction, and the expression levels and cellular distribution of acid base and electrolyte transporters in the proximal tubule, collecting duct and small intestine were examined by western blots, northern blots and/or immunofluorescence labeling...
February 21, 2018: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29469690/non-canonical-escherichia-coli-transcripts-lacking-a-shine-dalgarno-motif-have-very-different-translational-efficiencies-and-do-not-form-a-coherent-group
#14
Petra Ludwig, Madeleine Huber, Matthias Lehr, Marius Wegener, Karolin Zerulla, Christian Lange, Joerg Soppa
Translation initiation in 50-70 % of transcripts in Escherichia coli requires base pairing between the Shine-Dalgarno (SD) motif in the mRNA and the anti-SD motif at the 3' end of the 16S rRNA. However, 30-50 % of E. coli transcripts are non-canonical and are not preceded by an SD motif. The 5' ends of 44 E. coli transcripts were determined, all of which contained a 5'-UTR (no leaderless transcripts), but only a minority contained an SD motif. The 5'-UTR lengths were compared with those listed in RegulonDB and reported in previous publications, and the identities and differences were obtained in all possible combinations...
February 22, 2018: Microbiology
https://www.readbyqxmd.com/read/29455023/an-innovative-paradigm-of-methods-in-micrornas-detection-highlighting-dnazymes-the-illuminators
#15
REVIEW
Mojdeh Mahdiannasser, Zahra Karami
miRNAs are short regulatory sequences that monitor gene expression at post-transcriptional level. miRNAs play outstanding roles in cellular processes and their altered expression or aberrant function has been reported in diseases such as cancer. Furthermore, some of them serve as biomarkers for prognosis, diagnosis, and prediction of drug effectiveness as well as probable utility in the field of personalized medicine. As a result, proper and sensitive detection of miRNAs is a crucial matter. Traditional approaches including Northern blotting, Microarray, and Quantitative reverse transcription polymerase chain reaction have been extensively employed by means of miRNA detection for decades...
February 8, 2018: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/29453091/identification-of-mirnas-and-their-targets-in-maize-in-response-to-sugarcane-mosaic-virus-infection
#16
Zihao Xia, Zhenxing Zhao, Mingjun Li, Ling Chen, Zhiyuan Jiao, Yuanhua Wu, Tao Zhou, Weichang Yu, Zaifeng Fan
MicroRNAs (miRNAs) are endogenous non-coding small RNAs that play essential regulatory roles in plant development and environmental stress responses. Maize (Zea mays L.) is a global economically important food and forage crop. To date, a number of maize miRNAs have been identified as being involved in plant development and stress responses. However, the miRNA-mediated gene regulatory networks responsive to virus infections in maize remain largely unknown. In this study, the profiles of small RNAs in buffer- and Sugarcane mosaic virus (SCMV)-inoculated maize plants were obtained by high-throughput sequencing, respectively...
January 31, 2018: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/29441981/long-non-coding-rna-sox4-promotes-proliferation-and-migration-by-activating-wnt-%C3%AE-catenin-signaling-pathway-in-osteosarcoma
#17
Zhoubin Tian, Guang Yang, Peng Jiang, Laibo Zhang, Jian Wang, Shui Sun
Osteosarcoma is a bone tumor without effective treatment in the world. Recently, long non-coding RNAs (lncRNAs) are considered as essential regulators in cancer progression. LncSox4 plays crucial roles in liver tumor-initiating cells self-renewal and tumor initiation. However, the effect of lncSox4 in osteosarcoma remains largely unclear. Quantitative real-time PCR (qRT-PCR) and Northern blot were performed to detect lncSox4 expressions in osteosarcoma. The functions of lncSox4 in osteosarcoma were determined using cell viability and migration assays...
September 1, 2017: Die Pharmazie
https://www.readbyqxmd.com/read/29440670/characterization-of-conserved-and-novel-micrornas-in-lilium-lancifolium-thunb-by-high-throughput-sequencing
#18
Xiangfeng He, Awraris Getachew Shenkute, Wenhe Wang, Shufa Xu
MicroRNAs (miRNAs) are among the class of noncoding small RNA molecules and play a crucial role in post-transcriptional regulation in plants. Although Lilium is one of the most popular ornamental flowers worldwide, however, there is no report on miRNAs identification. In the present study, therefore, miRNAs and their targets were identified from flower, leaf, bulblet and bulb of Lilium lancifolium Thunb. by high-throughput sequencing and bioinformatics analysis. In this study, a total of 38 conserved miRNAs belonging to 17 miRNA families and 44 novel miRNAs were identified...
February 13, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29440318/monitoring-the-5-methoxycarbonylmethyl-2-thiouridine-mcm5s2u-modification-in-eukaryotic-trnas-via-the-gamma-toxin-endonuclease
#19
Jenna M Lentini, Jillian Ramos, Dragony Fu
The post-transcriptional modification of tRNA at the wobble position is a universal process occurring in all domains of life. In eukaryotes, the wobble uridine of particular tRNAs is transformed to the 5-methoxycarbonylmethyl-2-thiouridine (mcm5s2U) modification which is critical for proper mRNA decoding and protein translation. However, current methods to detect mcm5s2U are technically challenging and/or require specialized instrumental expertise. Here, we show that gamma-toxin endonuclease from the yeast Kluyveromyces lactis can be used as a probe for assaying mcm5s2U status in the tRNA of diverse eukaryotic organisms ranging from protozoans to mammalian cells...
February 13, 2018: RNA
https://www.readbyqxmd.com/read/29434609/frg3-a-target-of-slmir482e-3p-provides-resistance-against-the-fungal-pathogen-fusarium-oxysporum-in-tomato
#20
Hui-Min Ji, Min Zhao, Ying Gao, Xin-Xin Cao, Hui-Ying Mao, Yi Zhou, Wen-Yu Fan, Katherine A Borkovich, Shou-Qiang Ouyang, Peng Liu
The vast majority of plant disease resistance (R) genes encode nucleotide binding site-leucine-rich repeat (NBS-LRR) proteins, which specifically determine the plant immune response and have been demonstrated to be targets of several microRNA (miRNA) families. The fungus Fusarium oxysporum f. sp. lycopersici (FOL) causes vascular wilt disease in tomato worldwide. Here, we explored a possible role for FGR3 in tomato defense against FOL. FRG3 is a predicted NBS-LRR like gene that is targeted by slmiR482e-3p, a member of slmiR482 miRNA family...
2018: Frontiers in Plant Science
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