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https://www.readbyqxmd.com/read/28103012/a-bi-modal-cationic-and-water-soluble-calix-4-arene-conjugate-design-synthesis-characterization-and-transfection-of-rfp-encoded-plasmid-in-cancer-cells
#1
Kushal Samanta, Dnyanesh S Ranade, Aekta Upadhyay, Prasad Padmakar Kulkarni, Chebrolu Pulla Rao
A new bimodal fluorescent cationic calix[4]arene (L1) conjugate has been synthesized in multiple steps and well characterized by NMR and ESI-MS techniques. L1 has been investigated for its DNA binding ability by various spectroscopy techniques like, absorption, fluorescence and circular dichroism (CD). The formation of L1-DNA complex has been confirmed by the gel electrophoresis in presence of incremental concentration of L1. To visualize the packing of the plasmid (pBR322), detailed tapping mode AFM study has been performed, which revealed blob like structure of plasmid upon addition of the incremental amount of L1...
January 19, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28102851/cellular-prion-protein-mediates-early-apoptotic-proteome-alternation-and-phospho-modification-in-human-neuroblastoma-cells
#2
Saima Zafar, Christina Behrens, Hassan Dihazi, Matthias Schmitz, Inga Zerr, Walter J Schulz-Schaeffer, Sanja Ramljak, Abdul R Asif
Anti-apoptotic properties of physiological and elevated levels of the cellular prion protein (PrP(c)) under stress conditions are well documented. Yet, detrimental effects of elevated PrP(c) levels under stress conditions, such as exposure to staurosporine (STS) have also been described. In the present study, we focused on discerning early apoptotic STS-induced proteome and phospho-proteome changes in SH-SY5Y human neuroblastoma cells stably transfected either with an empty or PRNP-containing vector, expressing physiological or supraphysiological levels of PrP(c), respectively...
January 19, 2017: Cell Death & Disease
https://www.readbyqxmd.com/read/28102395/sesamol-ameliorates-high-fat-and-high-fructose-induced-cognitive-defects-via-improving-insulin-signaling-disruption-in-the-central-nervous-system
#3
Zhigang Liu, Yali Sun, Qinglian Qiao, Tong Zhao, Wentong Zhang, Bo Ren, Qian Liu, Xuebo Liu
Sesamol, a nutritional component from sesame, possesses antioxidant, lipid lowering and antidepressant activities. Nonetheless, few studies report its effects on high-energy-dense diet-induced cognitive loss. The present research aimed to elucidate the action of sesamol on high-fat and high-fructose (HFFD) "western"-diet-induced central nervous system insulin resistance and learning and memory impairment, and further determined the possible underlying mechanism. 3 month-old C57BL/6J mice were divided into 3 groups with/without sesamol in the drinking water (0...
January 19, 2017: Food & Function
https://www.readbyqxmd.com/read/28101920/quantitative-proteomic-analysis-of-hiv-1-tat-induced-dysregulation-in-sh-sy5y-neuroblastoma-cells
#4
Tariq Ganief, Putuma Gqamana, Shaun Garnett, Jackie Hoare, Dan J Stein, John Joska, Nelson Soares, Jonathan M Blackburn
Despite affecting up to 70% of HIV-positivepatients and being the leading cause of dementia in patients under 40 years, the molecular mechanisms involved in the onset of HIV-associated neurocognitive disorders (HAND) are not well understood. To address this, we performed SILAC-based quantitative proteomic analysis on HIV-Tat treated SH-SY5Y neuroblastoma cells. Isolated protein was fractionated by SDS-PAGE and analysed by nLC-MS/MS on an Orbitrap Velos. Using MaxQuant, we identified and quantified 3077 unique protein groups, of which 407 were differentially regulated...
January 19, 2017: Proteomics
https://www.readbyqxmd.com/read/28099881/mesencephalic-astrocyte-derived-neurotrophic-factor-alleviated-6-ohda-induced-cell-damage-via-ros-ampk-mtor-mediated-autophagic-inhibition
#5
Jingxing Zhang, Qiong Cai, Ming Jiang, Yigang Liu, Hua Gu, Jia Guo, Hui Sun, Jianmin Fang, Lingjing Jin
Autophagy and apoptosis are commonly involved in the dopaminergic neuron damage in the pathogenesis of Parkinson's disease. Recently, the autophagy pathway is thought to be critical to the process of PD. Therefore, the regulation of autophagy may be a potential strategy for PD treatment. Mesencephalic astrocyte-derived neurotrophic factor (MANF) has been reported to have neuroprotective effects through anti-apoptosis, anti-oxidative, and anti-inflammatory mechanisms in PD. In this study, we investigated the role of autophagy system in MANF-mediated neuroprotection against 6-hydroxydopamine (6-OHDA)-induced neurotoxicity...
January 15, 2017: Experimental Gerontology
https://www.readbyqxmd.com/read/28098253/the-clinically-approved-antiviral-drug-sofosbuvir-inhibits-zika-virus-replication
#6
Carolina Q Sacramento, Gabrielle R de Melo, Caroline S de Freitas, Natasha Rocha, Lucas Villas Bôas Hoelz, Milene Miranda, Natalia Fintelman-Rodrigues, Andressa Marttorelli, André C Ferreira, Giselle Barbosa-Lima, Juliana L Abrantes, Yasmine Rangel Vieira, Mônica M Bastos, Eduardo de Mello Volotão, Estevão Portela Nunes, Diogo A Tschoeke, Luciana Leomil, Erick Correia Loiola, Pablo Trindade, Stevens K Rehen, Fernando A Bozza, Patrícia T Bozza, Nubia Boechat, Fabiano L Thompson, Ana M B de Filippis, Karin Brüning, Thiago Moreno L Souza
Zika virus (ZIKV) is a member of the Flaviviridae family, along with other agents of clinical significance such as dengue (DENV) and hepatitis C (HCV) viruses. Since ZIKV causes neurological disorders during fetal development and in adulthood, antiviral drugs are necessary. Sofosbuvir is clinically approved for use against HCV and targets the protein that is most conserved among the members of the Flaviviridae family, the viral RNA polymerase. Indeed, we found that sofosbuvir inhibits ZIKV RNA polymerase, targeting conserved amino acid residues...
January 18, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28095495/dna-methylation-analysis-of-brd1-promoter-regions-and-the-schizophrenia-rs138880-risk-allele
#7
Mads Dyrvig, Per Qvist, Jacek Lichota, Knud Larsen, Mette Nyegaard, Anders D Børglum, Jane H Christensen
The bromodomain containing 1 gene, BRD1 is essential for embryogenesis and CNS development. It encodes a protein that participates in histone modifying complexes and thereby regulates the expression of a large number of genes. Genetic variants in the BRD1 locus show association with schizophrenia and bipolar disorder and risk alleles in the promoter region correlate with reduced BRD1 expression. Insights into the transcriptional regulation of BRD1 and the pathogenic mechanisms associated with BRD1 risk variants, however, remain sparse...
2017: PloS One
https://www.readbyqxmd.com/read/28094965/taxodikaloids-a-and-b-two-dimeric-abietane-type-diterpenoids-from-taxodium-ascendens-possessing-an-oxazoline-ring-linkage
#8
Xing-Hao Huang, Ling-Xue Tao, Chang-Qiang Ke, Chunping Tang, Hai-Yan Zhang, Yang Ye, Li-Gen Lin, Sheng Yao
Two dimeric diterpenoids, taxodikaloids A (1) and B (2), have been identified from the seeds of Taxodium ascendens. The diterpenoid structures were established on the basis of comprehensive spectroscopic analysis, and the absolute configuration of taxodikaloid A (1) was further confirmed by single-crystal X-ray diffraction. Both structures feature an unprecedented oxazoline ring linkage connecting two abietane diterpenoid monomers. A plausible biosynthetic pathway for compounds 1 and 2 is proposed. Both compounds show potential neuroprotective activity against Aβ25-35-induced damage in SH-SY5Y cells...
January 17, 2017: Organic Letters
https://www.readbyqxmd.com/read/28089584/pterostilbene-attenuates-high-glucose-induced-oxidative-injury-in-hippocampal-neuronal-cells-by-activating-nuclear-factor-erythroid-2-related-factor-2
#9
Yang Yang, Chongxi Fan, Bodong Wang, Zhiqiang Ma, Dongjin Wang, Bing Gong, Shouyin Di, Shuai Jiang, Yue Li, Tian Li, Zhi Yang, Erping Luo
In the present study, neuroblastoma (SH-SY5Y) cells were used to investigate the mechanisms mediating the potential protective effects of pterostilbene (PTE) against mitochondrial metabolic impairment and oxidative stress induced by hyperglycemia for mimicking the diabetic encephalopathy. High glucose medium (100 mM) decreased cellular viability after 24 h incubation which was evidenced by: (i) reduced mitochondrial complex I and III activities; (ii) reduced mitochondrial cytochrome C; (iii) increased reactive oxygen species (ROS) generation; (iv) decreased mitochondrial membrane potential (ΔΨm); and (v) increased lactate dehydrogenase (LDH) levels...
January 9, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28079261/effect-of-parthanatos-on-ropivacaine-induced-damage-in-sh-sy5y-cells
#10
Ting Zheng, Chun-Ying Zheng, Xiao-Chun Zheng, Ruo-Guang Zhao, Yan-Qing Chen
Ropivacaine is one of the most common but toxic local anesthetics, and the mechanisms underlying its neurotoxicity are still largely unknown. This study was conducted to prepare a ropivacaine-induced neuronal injury model and research the effects of ropivacaine on PARP-1 activation and NAD(+) depletion. The cell death and apoptosis of ropivacaine-induced SH-SY5Y cells were detected with flow cytometry. The lactate dehydrogenase cycling reaction measured the NAD(+) level, and Western blots were used to analyze the expression levels of PARP-1 and AIF after ropivacaine treatments with different concentrations and durations...
January 12, 2017: Clinical and Experimental Pharmacology & Physiology
https://www.readbyqxmd.com/read/28077289/vitamin-d3-protects-against-a%C3%AE-peptide-cytotoxicity-in-differentiated-human-neuroblastoma-sh-sy5y-cells-a-role-for-s1p1-p38mapk-atf4-axis
#11
Federica Pierucci, Mercedes Garcia-Gil, Alessia Frati, Francesca Bini, Maria Martinesi, Eleonora Vannini, Marco Mainardi, Federico Luzzati, Paolo Peretto, Matteo Caleo, Elisabetta Meacci
Besides its classical function of bone metabolism regulation, 1A,25-dihydroxyvitamin D3 (1,25(OH)2D3), acts on a variety of tissues including the nervous system, where the hormone plays an important role as neuroprotective, antiproliferating and differentiating agent. Sphingolipids are bioactive lipids that play critical and complex roles in regulating cell fate. In the present paper we have investigated whether sphingolipids are involved in the protective action of 1,25(OH)2D3. We have found that 1,25(OH)2D3 prevents amyloid-β peptide (Aβ(1-42)) cytotoxicity both in differentiated SH-SY5Y human neuroblastoma cells and in vivo...
January 7, 2017: Neuropharmacology
https://www.readbyqxmd.com/read/28075401/anti-neuroblastoma-properties-of-a-recombinant-sunflower-lectin
#12
Marcela Pinedo, Melanie Genoula, María Ximena Silveyra, André De Oliveira Carvalho, Mariana Regente, Marianela Del Río, Júlia Ribeiro Soares, Valdirene Moreira Gomes, Laura De La Canal
According to their sugar recognition specificity, plant lectins are proposed as bioactive proteins with potential in cancer treatment and diagnosis. Helja is a mannose-specific jacalin-like lectin from sunflower which was shown to inhibit the growth of certain fungi. Here, we report its recombinant expression in a prokaryotic system and its activity in neurobalstoma cells. Helja coding sequence was fused to the pET-32 EK/LIC, the enterokinase/Ligation-independent cloning vector and a 35 kDa protein was obtained in Escherichia coli representing Helja coupled to thioredoxin (Trx)...
January 10, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28073699/activation-of-nrf2-attenuates-carbonyl-stress-induced-by-methylglyoxal-in-human-neuroblastoma-cells-increase-in-gsh-levels-is-a-critical-event-for-the-detoxification-mechanism
#13
Shoichi Nishimoto, Shin Koike, Naho Inoue, Toshihiro Suzuki, Yuki Ogasawara
The present study focused on the methylglyoxal (MG) detoxification mechanism in neuroblastoma cells. The involvement of nuclear factor erythroid 2-related factor 2 (Nrf2)/Kelch-like ECH-associated protein 1 (Keap1) pathway as a defense response against the formation of MG-modified proteins, which is well-known evidence of carbonyl stress, was also examined. We found that MG treatment resulted in accumulation of modified proteins bearing the structure of advanced glycation end products (AGEs) derived from MG in SH-SY5Y cells...
January 7, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28073324/3-methoxy-aroylhydrazones-free-radicals-scavenging-anticancer-and-cytoprotective-potency
#14
Nadya Hristova-Avakumova, Krassimira Yoncheva, Boryana Nikolova-Mladenova, Trayko Traykov, Georgi Momekov, Vera Hadjimitova
OBJECTIVE: This study aimed to determine the capability of newly designed 3-methoxy derivatives of salicylaldehyde benzoylhydrazone to influence the oxidative stress processes and to test their in vitro cytotoxicity. METHODS: We have used chemiluminescent and spectrophotometric model systems containing different types of reactive oxygen species (OH(●), OCl(─) and O2(─●)). The hydrazones effect on the viability of Hep-G2, HEK-293 and SH-SY5Y cell lines was determined via MTT assay...
January 10, 2017: Redox Report: Communications in Free Radical Research
https://www.readbyqxmd.com/read/28069584/role-of-norepinephrine-in-a%C3%AE-related-neurotoxicity-dual-interactions-with-tyr10-and-snk-26-28-of-a%C3%AE
#15
Mengmeng Liu, Liping Wan, Yannan Bin, Juan Xiang
With their capability to inhibit the formation of amyloid-β peptide (Aβ) fibril, norepinephrine (NE), and other catechol derivatives have been considered for the potential treatment of Alzheimer's disease (AD). Such treatment, however, remains debatable because of the diverse functions of Aβ and NE in AD pathology. Moreover, the complicated oxidation accompanying NE has caused the majority of the previous research to focus on the binding of NE oxides onto Aβ. The molecular mechanism by which Aβ interacts with the reduction state of NE, which is correlated with the brain function, should be urgently explored...
January 8, 2017: Acta Biochimica et Biophysica Sinica
https://www.readbyqxmd.com/read/28069442/novel-signaling-of-dynorphin-at-%C3%AE%C2%BA-opioid-receptor-bradykinin-b2-receptor-heterodimers
#16
Bingyuan Ji, Haiqing Liu, Rumin Zhang, Yunlu Jiang, Chunmei Wang, Sheng Li, Jing Chen, Bo Bai
The κ-opioid receptor (KOR) and bradykinin B2 receptor (B2R) are involved in a variety of important physiological processes and share many similar characteristics in terms of their distribution and functions in the nervous system. We first demonstrated the endogenous expression of KOR and B2R in human SH-SY5Y cells and their co-localization on the membrane of human embryonic kidney 293 (HEK293) cells. Bioluminescence and fluorescence resonance energy transfer and the proximity ligation assay were exploited to demonstrate the formation of functional KOR and B2R heteromers in transfected cells...
January 7, 2017: Cellular Signalling
https://www.readbyqxmd.com/read/28067045/neurotoxicity-of-%C3%AE-keto-amphetamines-deathly-mechanisms-elicited-by-methylone-and-mdpv-in-human-dopaminergic-sh-sy5y-cells
#17
Maria Joao Valente, Maria Lourdes Bastos, Eduarda Fernandes, Félix Carvalho, Paola Guedes de Pinho, Marcia Carvalho
Synthetic cathinones (β-keto amphetamines) act as potent CNS stimulants similarly to classical amphetamines, which raise concerns about their potential neurotoxic effects. The present in vitro study aimed to explore and compare the mechanisms underlying the neurotoxicity of two commonly abused cathinone derivatives, 3,4-methylenedioxymethcathinone (methylone) and 3,4-methylenedioxypyrovalerone (MDPV), with those of 3,4-methylenedioxymethamphetamine (MDMA), using undifferentiated and differentiated SH-SY5Y cells...
January 9, 2017: ACS Chemical Neuroscience
https://www.readbyqxmd.com/read/28065858/role-of-transglutaminase-2-in-pac1-receptor-mediated-protection-against-hypoxia-induced-cell-death-and-neurite-outgrowth-in-differentiating-n2a-neuroblastoma-cells
#18
Alanood S Algarni, Alan J Hargreaves, John M Dickenson
The PAC1 receptor and tissue transglutaminase (TG2) play important roles in neurite outgrowth and modulation of neuronal cell survival. In this study, we investigated the regulation of TG2 activity by the PAC1 receptor in retinoic acid-induced differentiating N2a neuroblastoma cells. TG2 transamidase activity was determined using an amine incorporation and a peptide cross linking assay. In situ TG2 activity was assessed by visualising the incorporation of biotin-X-cadaverine using confocal microscopy. TG2 phosphorylation was monitored via immunoprecipitation and Western blotting...
January 5, 2017: Biochemical Pharmacology
https://www.readbyqxmd.com/read/28063130/glucocorticoids-curtail-stimuli-induced-creb-phosphorylation-in-trh-neurons-through-interaction-of-the-glucocorticoid-receptor-with-the-catalytic-subunit-of-protein-kinase-a
#19
Israim Sotelo-Rivera, Antonieta Cote-Vélez, Rosa-María Uribe, Jean-Louis Charli, Patricia Joseph-Bravo
PURPOSE: Corticosterone prevents cold-induced stimulation of thyrotropin-releasing hormone (Trh) expression in rats, and the stimulatory effect of dibutyryl cyclic-adenosine monophosphate (dB-cAMP) on Trh transcription in hypothalamic cultures. We searched for the mechanism of this interference. METHODS: Immunohistochemical analyses of phosphorylated cAMP-response element binding protein (pCREB) were performed in the paraventricular nucleus (PVN) of Wistar rats, and in cell cultures of 17-day old rat hypothalami, or neuroblastoma SH-SY5Y cells...
January 6, 2017: Endocrine
https://www.readbyqxmd.com/read/28062998/high-glucose-enhances-isoflurane-induced-neurotoxicity-by-regulating-trpc-dependent-calcium-influx
#20
ZhongJie Liu, ChangQing Ma, Wei Zhao, QingGuo Zhang, Rui Xu, HongFei Zhang, HongYi Lei, ShiYuan Xu
Isoflurane is a commonly used inhalational anesthetic that can induce neurotoxicity via elevating cytosolic calcium (Ca(2+)). High glucose regulates the expression of a family of non-selective cation channels termed transient receptor potential canonical (TRPC) channels that may contribute to Ca(2+) influx. In the present study, we investigated whether high glucose enhances isoflurane-induced neurotoxicity by regulating TRPC-dependent Ca(2+) influx. First, we evaluated toxic damage in mice primary cultured hippocampal neurons and human neuroblastoma cells (SH-SY5Y cells) after hyperglycemia and isoflurane exposure...
January 6, 2017: Neurochemical Research
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