Tingting Zhang, Daosheng Ai, Pingli Wei, Ying Xu, Zhanying Bi, Fengfei Ma, Fengzhi Li, Xing-Jun Chen, Zhaohuan Zhang, Xiaoxiao Zou, Zongpei Guo, Yue Zhao, Jun-Liszt Li, Meng Ye, Ziyan Feng, Xinshuang Zhang, Lijun Zheng, Jie Yu, Chunli Li, Tianqi Tu, Hongkui Zeng, Jianfeng Lei, Hongqi Zhang, Tao Hong, Li Zhang, Benyan Luo, Zhen Li, Chao Xing, Chenxi Jia, Lingjun Li, Wenzhi Sun, Woo-Ping Ge
The subcommissural organ (SCO) is a gland located at the entrance of the aqueduct of Sylvius in the brain. It exists in species as distantly related as amphioxus and humans, but its function is largely unknown. To explore its function, we compared transcriptomes of SCO and non-SCO brain regions and found three genes, Sspo , Car3 , and Spdef , that are highly expressed in the SCO. Mouse strains expressing Cre recombinase from endogenous promoter/enhancer elements of these genes were used to genetically ablate SCO cells during embryonic development, resulting in severe hydrocephalus and defects in neuronal migration and development of neuronal axons and dendrites...
March 31, 2024: bioRxiv