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Candida auris

Masako Mizusawa, Heather Miller, Rachel Green, Richard Lee, Mariann Durante, Rosalie Perkins, Caroline Hewitt, Patricia J Simner, Karen C Carroll, Randall T Hayden, Sean X Zhang
Candida auris, an emerging multi-drug resistant yeast associated with high mortality, has been increasingly reported from outside of the US to cause outbreaks in the hospital settings (1).….
November 23, 2016: Journal of Clinical Microbiology
Snigdha Vallabhaneni, Alex Kallen, Sharon Tsay, Nancy Chow, Rory Welsh, Janna Kerins, Sarah K Kemble, Massimo Pacilli, Stephanie R Black, Emily Landon, Jessica Ridgway, Tara N Palmore, Adrian Zelzany, Eleanor H Adams, Monica Quinn, Sudha Chaturvedi, Jane Greenko, Rafael Fernandez, Karen Southwick, E Yoko Furuya, David P Calfee, Camille Hamula, Gopi Patel, Patricia Barrett, Patricia Lafaro, Elizabeth L Berkow, Heather Moulton-Meissner, Judith Noble-Wang, Ryan P Fagan, Brendan R Jackson, Shawn R Lockhart, Anastasia P Litvintseva, Tom M Chiller
Candida auris, an emerging fungus that can cause invasive infections, is associated with high mortality and is often resistant to multiple antifungal drugs. C. auris was first described in 2009 after being isolated from external ear canal discharge of a patient in Japan (1). Since then, reports of C. auris infections, including bloodstream infections, have been published from several countries, including Colombia, India, Israel, Kenya, Kuwait, Pakistan, South Africa, South Korea, Venezuela, and the United Kingdom (2-7)...
November 11, 2016: MMWR. Morbidity and Mortality Weekly Report
Michael McCarthy
No abstract text is available yet for this article.
November 7, 2016: BMJ: British Medical Journal
Silke Schelenz, Ferry Hagen, Johanna L Rhodes, Alireza Abdolrasouli, Anuradha Chowdhary, Anne Hall, Lisa Ryan, Joanne Shackleton, Richard Trimlett, Jacques F Meis, Darius Armstrong-James, Matthew C Fisher
BACKGROUND: Candida auris is a globally emerging multidrug resistant fungal pathogen causing nosocomial transmission. We report an ongoing outbreak of C. auris in a London cardio-thoracic center between April 2015 and July 2016. This is the first report of C. auris in Europe and the largest outbreak so far. We describe the identification, investigation and implementation of control measures. METHODS: Data on C. auris case demographics, environmental screening, implementation of infection prevention/control measures, and antifungal susceptibility of patient isolates were prospectively recorded then analysed retrospectively...
2016: Antimicrobial Resistance and Infection Control
Tae-Hyoung Kim, Oh Joo Kweon, Hye Ryoun Kim, Mi-Kyung Lee
Recently, several studies have revealed that commercial microbial identification systems do not accurately identify the uncommon causative species of candidiasis, including Candida famata, Meyerozyma guilliermondii, and C. auris. We investigated the accuracy of species-level identification in a collection of clinical isolates previously identified as C. famata (N=38), C. lusitaniae (N=12), and M. guilliermondii (N=5) by the Vitek 2 system. All 55 isolates were re-analyzed by the Phoenix system (Becton Dickinson Diagnostics), two matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) analyzers (a Vitek MS and a Bruker Biotyper), and by sequencing of internal transcribed space (ITS) regions or 26S rRNA gene D1/D2 domains...
October 6, 2016: Journal of Microbiology and Biotechnology
A Chowdhary, A Voss, J F Meis
Since being first reported in an ear swab in 2009, and in blood cultures in 2011, invasive infections with Candida auris have been reported in many countries across several continents. We review current knowledge of the epidemiology of this emerging multidrug-resistant pathogen. The importance of species identification and the inadequacies of many widely used identification systems are considered. We recommend that hospitals develop their own policies for the prevention and control of infections with this pathogen...
August 15, 2016: Journal of Hospital Infection
C Sharma, N Kumar, R Pandey, J F Meis, A Chowdhary
Candida auris is an emerging multidrug resistant yeast that causes nosocomial fungaemia and deep-seated infections. Notably, the emergence of this yeast is alarming as it exhibits resistance to azoles, amphotericin B and caspofungin, which may lead to clinical failure in patients. The multigene phylogeny and amplified fragment length polymorphism typing methods report the C. auris population as clonal. Here, using whole genome sequencing analysis, we decipher for the first time that C. auris strains from four Indian hospitals were highly related, suggesting clonal transmission...
September 2016: New Microbes and New Infections
Andrew M Borman, Adrien Szekely, Elizabeth M Johnson
Candida auris, first described in 2009, has since emerged as an important, multidrug-resistant, nosocomial agent of candidemia, with large outbreaks reported worldwide and high mortality rates associated with therapeutic failure. The current study employed C. auris isolates from a variety of centers in the United Kingdom to evaluate the pathogenicity of this emerging pathogen compared to that of other common pathogenic yeast species in the invertebrate Galleria mellonella infection model. We showed that C...
July 2016: MSphere
Belinda Calvo, Analy S A Melo, Armindo Perozo-Mena, Martin Hernandez, Elaine Cristina Francisco, Ferry Hagen, Jacques F Meis, Arnaldo Lopes Colombo
OBJECTIVES: Characterization of a hospital outbreak of Candida auris candidemia that involved 18 critically ill patients in Venezuela. METHOD: Bloodstream isolates of C. auris obtained from 18 patients admitted at a medical center in Maracaibo, between March, 2012 and July, 2013 were included. Species identification was confirmed by ITS rDNA sequencing. Isolates were subsequently typed by amplified fragment length polymorphism fingerprinting (AFLP). Susceptibility testing was performed according to CLSI...
October 2016: Journal of Infection
Rafaella C Grenfell, Afonso R da Silva Junior, Gilda M B Del Negro, Regina B Munhoz, Viviane M F Gimenes, Diego M Assis, Anna C Rockstroh, Adriana L Motta, Flavia Rossi, Luiz Juliano, Gil Benard, João N de Almeida Júnior
Candida haemulonii is now considered a complex of two species and one variety: C. haemulonii sensu stricto, Candida duobushaemulonii and the variety C. haemulonii var. vulnera. Identification (ID) of these species is relevant for epidemiological purposes and for therapeutic management, but the different phenotypic commercial systems are unable to provide correct species ID for these emergent pathogens. Hence, we evaluated the MALDI-TOF MS performance for the ID of C. haemulonii species, analyzing isolates/strains of C...
2016: Frontiers in Microbiology
Victoria Girard, Sandrine Mailler, Marion Chetry, Céline Vidal, Géraldine Durand, Alex van Belkum, Arnaldo L Colombo, Ferry Hagen, Jacques F Meis, Anuradha Chowdhary
Candida auris is an emerging antifungal resistant yeast species causing nosocomial and invasive infections, emphasising the need of improved diagnostics and epidemiological typing methods. We show that MALDI-TOF VITEK-MS followed by amplified length polymorphisms allows for accurate species identification and subsequent epidemiological characterisation of strains encountered during potential outbreaks.
August 2016: Mycoses
A Prakash, C Sharma, A Singh, P Kumar Singh, A Kumar, F Hagen, N P Govender, A L Colombo, J F Meis, A Chowdhary
Candida auris is a multidrug-resistant nosocomial bloodstream pathogen that has been reported from Asian countries and South Africa. Herein, we studied the population structure and genetic relatedness among 104 global C. auris isolates from India, South Africa and Brazil using multilocus sequence typing (MLST), amplified fragment length polymorphism (AFLP) fingerprinting and matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS). RPB1, RPB2 and internal transcribed spacer (ITS) and D1/D2 regions of the ribosomal DNA were sequenced for MLST...
March 2016: Clinical Microbiology and Infection
Sharanya Chatterjee, Shuba Varshini Alampalli, Rishi Kumar Nageshan, Sivarajan T Chettiar, Sangeeta Joshi, Utpal S Tatu
BACKGROUND: Candida auris is a multidrug resistant, emerging agent of fungemia in humans. Its actual global distribution remains obscure as the current commercial methods of clinical diagnosis misidentify it as C. haemulonii. Here we report the first draft genome of C. auris to explore the genomic basis of virulence and unique differences that could be employed for differential diagnosis. RESULTS: More than 99.5 % of the C. auris genomic reads did not align to the current whole (or draft) genome sequences of Candida albicans, Candida lusitaniae, Candida glabrata and Saccharomyces cerevisiae; thereby indicating its divergence from the active Candida clade...
September 7, 2015: BMC Genomics
Cheshta Sharma, Nitin Kumar, Jacques F Meis, Rajesh Pandey, Anuradha Chowdhary
Candida auris is a multidrug-resistant yeast incriminated in a wide spectrum of invasive infections, especially in intensive care settings. The first draft genome sequence of C. auris, VPCI 479/P/13, from a case with fungemia was sequenced using the Illumina MiSeq platform. The estimated genome size is 12.3 Mb, with 6,675 coding sequences.
2015: Genome Announcements
Dharmendra Kumar, Tuhina Banerjee, Chandra Bhan Pratap, Ragini Tilak
Since the emergence of pathogenic non-albicans Candida species, a number of new isolates have been added to the list. One such unusual species is Candida auris (C. auris), recently isolated and studied in few reports. In this study, a case of vulvovaginitis caused by Candida auris incidentally identified by molecular methods using internal transcribed spacer polymerase chain reaction (ITS PCR) is described. Antifungal susceptibility testing revealed the isolate to be resistant to itraconazole (MIC ≥ 2 µg/ml) and expressed important virulence factors including phospholipase, proteinase and hemolysin activity...
April 2015: Journal of Infection in Developing Countries
Shallu Kathuria, Pradeep K Singh, Cheshta Sharma, Anupam Prakash, Aradhana Masih, Anil Kumar, Jacques F Meis, Anuradha Chowdhary
Candida auris is a multidrug-resistant yeast that causes a wide spectrum of infections, especially in intensive care settings. We investigated C. auris prevalence among 102 clinical isolates previously identified as Candida haemulonii or Candida famata by the Vitek 2 system. Internal transcribed spacer region (ITS) sequencing confirmed 88.2% of the isolates as C. auris, and matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) easily separated all related species, viz., C...
June 2015: Journal of Clinical Microbiology
Pedro Henrique de Azambuja Carvalho, Auri Rocha Duval, Fabio Renato Manzolli Leite, Fernanda Nedel, Wilson Cunico, Rafael Guerra Lund
OBJECTIVE: This work describes the anti-enzymatic activity of (7-chloroquinolin-4-yl)arylhydrazones against Candida albicans and examines their cytotoxicity. MATERIAL AND METHODS: Ten C. albicans strains [nine isolates and one azole-resistant standard strain (ATCC 62342)] were used to assess the anti-enzymatic activity. Fifteen compounds at sub-antifungal concentrations ranging from 12.5 to 100 µg/ml were assessed after a 30-min exposure. The strains were seeded onto petri dishes with selective agar media for aspartyl proteases (Saps) and phospholipases (PLs)...
2016: Journal of Enzyme Inhibition and Medicinal Chemistry
A K Ghosh, S Paul, P Sood, S M Rudramurthy, A Rajbanshi, T J Jillwin, A Chakrabarti
Few studies have systematically standardised and evaluated matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) for identification of yeasts from bloodstream infections. This is rapidly becoming pertinent for early identification of yeasts and appropriate antifungal therapy. We used 354 yeast strains identified by polymerase chain reaction (PCR) sequencing for standardisation and 367 blind clinical strains for validation of our MALDI-TOF MS protocols. We also evaluated different sample preparation methods and found the on-plate formic acid extraction method as most cost- and time-efficient...
April 2015: Clinical Microbiology and Infection
Arunaloke Chakrabarti, Prashant Sood, Shivaprakash M Rudramurthy, Sharon Chen, Harsimran Kaur, Malini Capoor, Deepinder Chhina, Ratna Rao, Vandana Kalwaje Eshwara, Immaculata Xess, Anupama J Kindo, P Umabala, Jayanthi Savio, Atul Patel, Ujjwayini Ray, Sangeetha Mohan, Ranganathan Iyer, Jagdish Chander, Anita Arora, Raman Sardana, Indranil Roy, B Appalaraju, Ajanta Sharma, Anjali Shetty, Neelam Khanna, Rungmei Marak, Sanjay Biswas, Shukla Das, B N Harish, Sangeeta Joshi, Deepak Mendiratta
PURPOSE: A systematic epidemiological study on intensive care unit (ICU)-acquired candidemia across India. METHOD: A prospective, nationwide, multicentric, observational study was conducted at 27 Indian ICUs. Consecutive patients who acquired candidemia after ICU admission were enrolled during April 2011 through September 2012. Clinical and laboratory variables of these patients were recorded. The present study is an analysis of data specific for adult patients...
February 2015: Intensive Care Medicine
A Chowdhary, V Anil Kumar, C Sharma, A Prakash, K Agarwal, R Babu, K R Dinesh, S Karim, S K Singh, F Hagen, J F Meis
Candida auris is a recently described rare agent of fungemia. It is notable for its antifungal resistance. A total of 15 C. auris isolates, originating from seven cases of fungemia, three cases of diabetic gangrenous foot, and one case of bronchopneumonia from a tertiary care hospital in south India, were investigated. All of the 15 isolates were identified by sequencing and 14 of these along with 12 C. auris isolates previously reported from two hospitals in Delhi, north India, two each from Japan and Korea were genotyped by amplified fragment length polymorphism (AFLP)...
June 2014: European Journal of Clinical Microbiology & Infectious Diseases
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