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Helicobacter hepaticus

Christelle Péré-Védrenne, Martina Prochazkova-Carlotti, Benoit Rousseau, Wencan He, Lucie Chambonnier, Elodie Sifré, Alice Buissonnière, Pierre Dubus, Francis Mégraud, Christine Varon, Armelle Ménard
Cytolethal distending toxins (CDTs) are common among pathogenic bacteria of the human and animal microbiota. CDTs exert cytopathic effets, via their active CdtB subunit. No clear description of those cytopathic effects has been reported at the cellular level in the target organs in vivo. In the present study, xenograft mouse models of colon and liver cell lines were set up to study the effects of the CdtB subunit of Helicobacter hepaticus. Conditional transgenic cell lines were established, validated in vitro and then engrafted into immunodeficient mice...
2017: Frontiers in Cellular and Infection Microbiology
C Wang, G Gong, A Sheh, S Muthupalani, E M Bryant, D A Puglisi, H Holcombe, E A Conaway, N A P Parry, V Bakthavatchalu, S P Short, C S Williams, G N Wogan, S R Tannenbaum, J G Fox, B H Horwitz
The risk of colon cancer is increased in patients with Crohn's disease and ulcerative colitis. Inflammation-induced DNA damage could be an important link between inflammation and cancer, although the pathways that link inflammation and DNA damage are incompletely defined. RAG2-deficient mice infected with Helicobacter hepaticus (Hh) develop colitis that progresses to lower bowel cancer. This process depends on nitric oxide (NO), a molecule with known mutagenic potential. We have previously hypothesized that production of NO by macrophages could be essential for Hh-driven carcinogenesis, however, whether Hh infection induces DNA damage in this model and whether this depends on NO has not been determined...
February 15, 2017: Mucosal Immunology
L Majlessi, F Sayes, J-F Bureau, A Pawlik, V Michel, G Jouvion, M Huerre, M Severgnini, C Consolandi, C Peano, R Brosch, E Touati, C Leclerc
Epidemiological and experimental observations suggest that chronic microbial colonization can impact the immune control of other unrelated pathogens contracted in a concomitant or sequential manner. Possible interactions between Mycobacterium tuberculosis infection and persistence of other bacteria have scarcely been investigated. Here we demonstrated that natural colonization of the digestive tract with Helicobacter hepaticus in mice is concomitant with modification of the gut microbiota, subclinical inflammation, and drastic impairment of immune control of the growth of subsequently administered M...
September 2017: Mucosal Immunology
Zhongming Ge, Yan Feng, Lili Ge, Nicola Parry, Sureshkumar Muthupalani, James G Fox
Multiple pathogenic Gram-negative bacteria produce the cytolethal distending toxin (CDT) with activity of DNase I; CDT can induce DNA double-strand breaks (DSBs), G2/M cell cycle arrest, and apoptosis in cultured mammalian cells. However, the link of CDT to in vivo tumorigenesis is not fully understood. In this study, 129/SvEv Rag2(-/-) mice were gavaged with wild-type Helicobacter hepatics 3B1(Hh) and its isogenic cdtB mutant HhcdtBm7, followed by infection for 10 and 20 weeks (WPI). HhCDT deficiency did not affect cecal colonization levels of HhcdtBm7, but attenuated severity of cecal pathology in HhcdtBm7-infected mice...
July 2017: Cellular Microbiology
Danmei Su, Yuanyang Nie, Airu Zhu, Zishuo Chen, Pengfei Wu, Li Zhang, Mei Luo, Qun Sun, Linbi Cai, Yuchen Lai, Zhixiong Xiao, Zhongping Duan, Sujun Zheng, Guihui Wu, Richard Hu, Hidekazu Tsukamoto, Aurelia Lugea, Zhenqui Liu, Stephen J Pandol, Yuan-Ping Han
Metabolic syndrome (MetS), characterized as obesity, insulin resistance, and non-alcoholic fatty liver diseases (NAFLD), is associated with vitamin D insufficiency/deficiency in epidemiological studies, while the underlying mechanism is poorly addressed. On the other hand, disorder of gut microbiota, namely dysbiosis, is known to cause MetS and NAFLD. It is also known that systemic inflammation blocks insulin signaling pathways, leading to insulin resistance and glucose intolerance, which are the driving force for hepatic steatosis...
2016: Frontiers in Physiology
Marie-Odile Husson, Delphine Ley, Céline Portal, Madeleine Gottrand, Thomas Hueso, Jean-Luc Desseyn, Frédéric Gottrand
OBJECTIVES: Although n-3 long-chain polyunsaturated fatty acids (n-3 LC-PUFAs) are used widely in the treatment of chronic inflammatory diseases, their effect in infectious disease requires a particular attention. METHODS: The present article discusses their anti-inflammatory and immune properties involved in the host defence and presents a systematic review of the effects of their oral administration on the prevention and outcome of experimental and clinical infections...
December 2016: Journal of Infection
Miaoli Wu, Dan Rao, Yujun Zhu, Jing Wang, Wen Yuan, Yu Zhang, Ren Huang, Pengju Guo
BACKGROUND: Enterohepatic Helicobacter species (EHS) are widespread in rodent species around the world. Several studies have demonstrated that infection with EHS can interfere with the outcomes of animal experiments in cancer research and significantly influence the study results. Therefore, it is essential to establish a rapid detection and identification of EHS for biomedical research using laboratory rodents. Our study aimed to develop a rapid and sensitive method to detect and distinguish five enterohepatic Helicobacter species...
April 2017: Helicobacter
Heping Qin, Guodu Tang, Ping Yi, Xinyi Pan, Huali Huang, Renjie Chang, Zhe Shi, Muhammad Aqeel Ashraf
The present study aimed to establish a genus-specific PCR-based assay to detect helicobacters using 16S rRNA gene as the target template. We designed the hemi-nested primers based on sequences of 16S rRNA gene of 34 types of Helicobacter species. The inclusivity, sensitivity, and specificity of the PCR assay using these primers were examined in three different models, comprising feces simulated samples, BLAB/c mice infection model and clinic patients samples. The detection sensitivity of Helicobacter pylori, Helicobacter hepaticus and Helicobacter bilis strains from feces simulated samples was all 102 CFU/ml...
May 2016: Saudi Pharmaceutical Journal: SPJ: the Official Publication of the Saudi Pharmaceutical Society
Verena Brucklacher-Waldert, Cristina Ferreira, Silvia Innocentin, Shraddha Kamdar, David R Withers, Marika C Kullberg, Marc Veldhoen
The discovery of Th17 cell plasticity, in which CD4(+) IL-17-producing Th17 cells give rise to IL-17/IFN-γ double-producing cells and Th1-like IFNγ(+) ex-Th17 lymphocytes, has raised questions regarding which of these cell types contribute to immunopathology during inflammatory diseases. In this study, we show using Helicobacter hepaticus-induced intestinal inflammation that IL-17A(Cre)- or Rag1(Cre)-mediated deletion of Tbx21 has no effect on the generation of IL-17/IFN-γ double-producing cells, but leads to a marked absence of Th1-like IFNγ(+) ex-Th17 cells...
June 15, 2016: Journal of Immunology: Official Journal of the American Association of Immunologists
Mathilde J H Girard-Madoux, Juliane L Ober-Blöbaum, Léa M M Costes, Junda M Kel, Dicky J Lindenbergh-Kortleve, Inge Brouwers-Haspels, Astrid P Heikema, Janneke N Samsom, Björn E Clausen
Although IL-10 promotes a regulatory phenotype of CD11c+ dendritic cells and macrophages in vitro, the role of IL-10 signaling in CD11c+ cells to maintain intestinal tolerance in vivo remains elusive. To this aim, we generated mice with a CD11c-specific deletion of the IL-10 receptor alpha (Cd11ccreIl10rafl/fl). In contrast to the colon, the small intestine of Cd11ccreIl10rafl/fl mice exhibited spontaneous crypt hyperplasia, increased numbers of intraepithelial lymphocytes and lamina propria T cells, associated with elevated levels of T cell-derived IFNγ and IL-17A...
May 31, 2016: Oncotarget
Christelle Péré-Védrenne, Bruno Cardinaud, Christine Varon, Iulia Mocan, Alice Buissonnière, Julien Izotte, Francis Mégraud, Armelle Ménard
Enterohepatic Helicobacter species are associated with several digestive diseases. Helicobacter pullorum is an emerging human foodborne pathogen, and Helicobacter hepaticus is a mouse pathogen; both species are associated with intestinal and/or hepatic diseases. They possess virulence factors, such as cytolethal distending toxin (CDT). Data indicate that CDT may be involved in chronic inflammatory responses, via its active subunit, CdtB. The proinflammatory properties of the CdtB of H. pullorum and H. hepaticus were assessed on human intestinal and hepatic epithelial cells in vitro...
June 15, 2016: Journal of Infectious Diseases
J Zimmermann, A A Kühl, M Weber, J R Grün, J Löffler, C Haftmann, R Riedel, P Maschmeyer, K Lehmann, K Westendorf, M-F Mashreghi, M Löhning, M Mack, A Radbruch, H D Chang
The transcription factor T-bet is highly expressed by Th cells isolated from the inflamed intestine of Crohn's disease patients, and has been regarded a critical driver of murine T cell-induced colitis. However, we show here that T-bet expression by Th cells is not required for the manifestation of T-cell-induced colitis in the presence of segmented filamentous bacteria and Helicobacter hepaticus. T-bet expression by Th cells controls their survival and localization, their repertoire of chemokine and chemokine receptor expression, the accumulation of monocytes and macrophages in the inflamed colon, and their differentiation to the M1 type, i...
February 17, 2016: Mucosal Immunology
Soraya J Kaewpitoon, Ryan A Loyd, Ratana Rujirakul, Sukij Panpimanmas, Likit Matrakool, Taweesak Tongtawee, Nusorn Kootanavanichpong, Prasit Pengsaa, Ponthip Kompor, Wasugree Chavengkun, Jirawoot Kujapun, Jun Norkaew, Sukanya Ponphimai, Natnapa Padchasuwan, Poowadol Polsripradist, Thawatchai Eksanti, Tanida Phatisena, Natthawut Kaewpitoon
Several infectious agents are considered to be causes of cancer in human, mainly hepatitis B and C viruses, high-risk human pailloma viruses, Helicobacter pylori, Clonorchis sinensis, and Opisthorchis viverrini. Here we described the evident research and the association between Helicobacter spp. and biliary tract cancer particularly cholangiocarcinoma (CCA). Global epidemiological studies have suggested that Helicobacter spp. are possible risk factors for biliary tract diseases. Molecular studies support a linkage of Helicobacter spp...
2016: Asian Pacific Journal of Cancer Prevention: APJCP
Jeroen Frank, Celia Dingemanse, Arnoud M Schmitz, Rolf H A M Vossen, Gert-Jan B van Ommen, Johan T den Dunnen, Els C Robanus-Maandag, Seyed Yahya Anvar
BACKGROUND: Immuno-compromised mice infected with Helicobacter typhlonius are used to model microbially inducted inflammatory bowel disease (IBD). The specific mechanism through which H. typhlonius induces and promotes IBD is not fully understood. Access to the genome sequence is essential to examine emergent properties of this organism, such as its pathogenicity. To this end, we present the complete genome sequence of H. typhlonius MIT 97-6810, obtained through single-molecule real-time sequencing...
2015: Frontiers in Microbiology
Oscar Medina-Contreras, Akihito Harusato, Hikaru Nishio, Kyle L Flannigan, Vu Ngo, Giovanna Leoni, Philipp-Alexander Neumann, Duke Geem, Loukia N Lili, Ravisankar A Ramadas, Benoit Chassaing, Andrew T Gewirtz, Jacob E Kohlmeier, Charles A Parkos, Jennifer E Towne, Asma Nusrat, Timothy L Denning
IL-1 family members are central mediators of host defense. In this article, we show that the novel IL-1 family member IL-36γ was expressed during experimental colitis and human inflammatory bowel disease. Germ-free mice failed to induce IL-36γ in response to dextran sodium sulfate (DSS)-induced damage, suggesting that gut microbiota are involved in its induction. Surprisingly, IL-36R-deficient (Il1rl2(-/-)) mice exhibited defective recovery following DSS-induced damage and impaired closure of colonic mucosal biopsy wounds, which coincided with impaired neutrophil accumulation in the wound bed...
January 1, 2016: Journal of Immunology: Official Journal of the American Association of Immunologists
Peter J Morrison, Sarah J Ballantyne, Sandy J Macdonald, John W J Moore, David Jenkins, Jill F Wright, Lynette A Fouser, Marika C Kullberg
Type 17 helper T-cell cytokines have been implicated in the pathogenesis of inflammatory bowel disease, a chronic condition affecting the gastrointestinal tract, but information regarding their contribution to pathology in different regions of the gut is lacking. By using a murine model of bacteria-induced typhlocolitis, we investigated the role of IL-17A, IL-17F, and IL-22 in cecal versus colonic inflammation. Cecal, but not colonic, pathology in C57BL/6 mice inoculated with Helicobacter hepaticus plus anti-IL-10 receptor (IL-10R) monoclonal antibody was exacerbated by co-administration of anti-IL-17A monoclonal antibody, suggesting a disease-protective role for IL-17A in the cecum...
December 2015: American Journal of Pathology
Avijit Ray, Sreemanti Basu, Raad Z Gharaibeh, Lydia C Cook, Ranjit Kumar, Elliot J Lefkowitz, Catherine R Walker, Casey D Morrow, Craig L Franklin, Terrence L Geiger, Nita H Salzman, Anthony Fodor, Bonnie N Dittel
It is clear that IL-10 plays an essential role in maintaining homeostasis in the gut in response to the microbiome. However, it is unknown whether IL-10 also facilitates immune homeostasis at distal sites. To address this question, we asked whether splenic immune populations were altered in IL-10-deficient (Il10(-/-)) mice in which differences in animal husbandry history were associated with susceptibility to spontaneous enterocolitis that is microbiome dependent. The susceptible mice exhibited a significant increase in splenic macrophages, neutrophils, and marginal zone (MZ) B cells that was inhibited by IL-10 signaling in myeloid, but not B cells...
October 1, 2015: Journal of Immunology: Official Journal of the American Association of Immunologists
Mark E Anderson, Emily C O'Brien, Emily N Grayek, James K Hermansen, Heather K Hunt
Current bacterial detection techniques are relatively slow, require bulky instrumentation, and usually require some form of specialized training. The gold standard for bacterial detection is culture testing, which can take several days to receive a viable result. Therefore, simpler detection techniques that are both fast and sensitive could greatly improve bacterial detection and identification. Here, we present a new method for the detection of the bacteria Helicobacter hepaticus using whispering-gallery mode (WGM) optical microcavity-based sensors...
2015: Biosensors
I C Arnold, S Mathisen, J Schulthess, C Danne, A N Hegazy, F Powrie
In inflammatory bowel diseases, a breakdown in host microbial interactions accompanies sustained activation of immune cells in the gut. Functional studies suggest a key role for interleukin-23 (IL-23) in orchestrating intestinal inflammation. IL-23 can be produced by various mononuclear phagocytes (MNPs) following acute microbial stimulation, but little is known about the key cellular sources of IL-23 that drive chronic intestinal inflammation. Here we have addressed this question using a physiological model of bacteria-driven colitis...
March 2016: Mucosal Immunology
Deepthi Nammi, Ravi C P K Srimath-Tirumala-Peddinti, Nageswara Rao R Neelapu
Helicobacter pylori colonize stomach, inducing gastritis, ulcers and gastric cancer. Drugs are used to relieve pain, but not H. pylori infections. Hence, there is a need for discovery of drug targets and drugs for H. pylori. An objective of this current study is to identify drug targets for H. pylori. RAST was used to compare genomes of 23 H. pylori strains with Homo sapiens sapiens, other Helicobacter species (H. acinonychis, H. hepaticus, H. mustalae) and among them, to identify 13471 unique genes. Bacterial genes which are non-homologous to humans and essential for pathogen are identified using BLASTp...
2016: Current Cancer Drug Targets
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