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Helicobacter hepaticus

Marie-Odile Husson, Delphine Ley, Céline Portal, Madeleine Gottrand, Thomas Hueso, Jean-Luc Desseyn, Frédéric Gottrand
OBJECTIVES: Although n-3 long-chain polyunsaturated fatty acids (n-3 LC-PUFAs) are used widely in the treatment of chronic inflammatory diseases, their effect in infectious disease requires a particular attention. METHODS: The present article discusses their anti-inflammatory and immune properties involved in the host defence and presents a systematic review of the effects of their oral administration on the prevention and outcome of experimental and clinical infections...
October 14, 2016: Journal of Infection
Miaoli Wu, Dan Rao, Yujun Zhu, Jing Wang, Wen Yuan, Yu Zhang, Ren Huang, Pengju Guo
BACKGROUND: Enterohepatic Helicobacter species (EHS) are widespread in rodent species around the world. Several studies have demonstrated that infection with EHS can interfere with the outcomes of animal experiments in cancer research and significantly influence the study results. Therefore, it is essential to establish a rapid detection and identification of EHS for biomedical research using laboratory rodents. Our study aimed to develop a rapid and sensitive method to detect and distinguish five enterohepatic Helicobacter species...
September 30, 2016: Helicobacter
Heping Qin, Guodu Tang, Ping Yi, Xinyi Pan, Huali Huang, Renjie Chang, Zhe Shi, Muhammad Aqeel Ashraf
The present study aimed to establish a genus-specific PCR-based assay to detect helicobacters using 16S rRNA gene as the target template. We designed the hemi-nested primers based on sequences of 16S rRNA gene of 34 types of Helicobacter species. The inclusivity, sensitivity, and specificity of the PCR assay using these primers were examined in three different models, comprising feces simulated samples, BLAB/c mice infection model and clinic patients samples. The detection sensitivity of Helicobacter pylori, Helicobacter hepaticus and Helicobacter bilis strains from feces simulated samples was all 102 CFU/ml...
May 2016: Saudi Pharmaceutical Journal: SPJ: the Official Publication of the Saudi Pharmaceutical Society
Verena Brucklacher-Waldert, Cristina Ferreira, Silvia Innocentin, Shraddha Kamdar, David R Withers, Marika C Kullberg, Marc Veldhoen
The discovery of Th17 cell plasticity, in which CD4(+) IL-17-producing Th17 cells give rise to IL-17/IFN-γ double-producing cells and Th1-like IFNγ(+) ex-Th17 lymphocytes, has raised questions regarding which of these cell types contribute to immunopathology during inflammatory diseases. In this study, we show using Helicobacter hepaticus-induced intestinal inflammation that IL-17A(Cre)- or Rag1(Cre)-mediated deletion of Tbx21 has no effect on the generation of IL-17/IFN-γ double-producing cells, but leads to a marked absence of Th1-like IFNγ(+) ex-Th17 cells...
June 15, 2016: Journal of Immunology: Official Journal of the American Association of Immunologists
Mathilde J H Girard-Madoux, Juliane L Ober-Blöbaum, Léa M M Costes, Junda M Kel, Dicky J Lindenbergh-Kortleve, Inge Brouwers-Haspels, Astrid P Heikema, Janneke N Samsom, Björn E Clausen
Although IL-10 promotes a regulatory phenotype of CD11c+ dendritic cells and macrophages in vitro, the role of IL-10 signaling in CD11c+ cells to maintain intestinal tolerance in vivo remains elusive. To this aim, we generated mice with a CD11c-specific deletion of the IL-10 receptor alpha (Cd11ccreIl10rafl/fl). In contrast to the colon, the small intestine of Cd11ccreIl10rafl/fl mice exhibited spontaneous crypt hyperplasia, increased numbers of intraepithelial lymphocytes and lamina propria T cells, associated with elevated levels of T cell-derived IFNγ and IL-17A...
May 31, 2016: Oncotarget
Christelle Péré-Védrenne, Bruno Cardinaud, Christine Varon, Iulia Mocan, Alice Buissonnière, Julien Izotte, Francis Mégraud, Armelle Ménard
Enterohepatic Helicobacter species are associated with several digestive diseases. Helicobacter pullorum is an emerging human foodborne pathogen, and Helicobacter hepaticus is a mouse pathogen; both species are associated with intestinal and/or hepatic diseases. They possess virulence factors, such as cytolethal distending toxin (CDT). Data indicate that CDT may be involved in chronic inflammatory responses, via its active subunit, CdtB. The proinflammatory properties of the CdtB of H. pullorum and H. hepaticus were assessed on human intestinal and hepatic epithelial cells in vitro...
June 15, 2016: Journal of Infectious Diseases
J Zimmermann, A A Kühl, M Weber, J R Grün, J Löffler, C Haftmann, R Riedel, P Maschmeyer, K Lehmann, K Westendorf, M-F Mashreghi, M Löhning, M Mack, A Radbruch, H D Chang
The transcription factor T-bet is highly expressed by Th cells isolated from the inflamed intestine of Crohn's disease patients, and has been regarded a critical driver of murine T cell-induced colitis. However, we show here that T-bet expression by Th cells is not required for the manifestation of T-cell-induced colitis in the presence of segmented filamentous bacteria and Helicobacter hepaticus. T-bet expression by Th cells controls their survival and localization, their repertoire of chemokine and chemokine receptor expression, the accumulation of monocytes and macrophages in the inflamed colon, and their differentiation to the M1 type, i...
February 17, 2016: Mucosal Immunology
Soraya J Kaewpitoon, Ryan A Loyd, Ratana Rujirakul, Sukij Panpimanmas, Likit Matrakool, Taweesak Tongtawee, Nusorn Kootanavanichpong, Prasit Pengsaa, Ponthip Kompor, Wasugree Chavengkun, Jirawoot Kujapun, Jun Norkaew, Sukanya Ponphimai, Natnapa Padchasuwan, Poowadol Polsripradist, Thawatchai Eksanti, Tanida Phatisena, Natthawut Kaewpitoon
Several infectious agents are considered to be causes of cancer in human, mainly hepatitis B and C viruses, high-risk human pailloma viruses, Helicobacter pylori, Clonorchis sinensis, and Opisthorchis viverrini. Here we described the evident research and the association between Helicobacter spp. and biliary tract cancer particularly cholangiocarcinoma (CCA). Global epidemiological studies have suggested that Helicobacter spp. are possible risk factors for biliary tract diseases. Molecular studies support a linkage of Helicobacter spp...
2016: Asian Pacific Journal of Cancer Prevention: APJCP
Jeroen Frank, Celia Dingemanse, Arnoud M Schmitz, Rolf H A M Vossen, Gert-Jan B van Ommen, Johan T den Dunnen, Els C Robanus-Maandag, Seyed Yahya Anvar
BACKGROUND: Immuno-compromised mice infected with Helicobacter typhlonius are used to model microbially inducted inflammatory bowel disease (IBD). The specific mechanism through which H. typhlonius induces and promotes IBD is not fully understood. Access to the genome sequence is essential to examine emergent properties of this organism, such as its pathogenicity. To this end, we present the complete genome sequence of H. typhlonius MIT 97-6810, obtained through single-molecule real-time sequencing...
2015: Frontiers in Microbiology
Oscar Medina-Contreras, Akihito Harusato, Hikaru Nishio, Kyle L Flannigan, Vu Ngo, Giovanna Leoni, Philipp-Alexander Neumann, Duke Geem, Loukia N Lili, Ravisankar A Ramadas, Benoit Chassaing, Andrew T Gewirtz, Jacob E Kohlmeier, Charles A Parkos, Jennifer E Towne, Asma Nusrat, Timothy L Denning
IL-1 family members are central mediators of host defense. In this article, we show that the novel IL-1 family member IL-36γ was expressed during experimental colitis and human inflammatory bowel disease. Germ-free mice failed to induce IL-36γ in response to dextran sodium sulfate (DSS)-induced damage, suggesting that gut microbiota are involved in its induction. Surprisingly, IL-36R-deficient (Il1rl2(-/-)) mice exhibited defective recovery following DSS-induced damage and impaired closure of colonic mucosal biopsy wounds, which coincided with impaired neutrophil accumulation in the wound bed...
January 1, 2016: Journal of Immunology: Official Journal of the American Association of Immunologists
Peter J Morrison, Sarah J Ballantyne, Sandy J Macdonald, John W J Moore, David Jenkins, Jill F Wright, Lynette A Fouser, Marika C Kullberg
Type 17 helper T-cell cytokines have been implicated in the pathogenesis of inflammatory bowel disease, a chronic condition affecting the gastrointestinal tract, but information regarding their contribution to pathology in different regions of the gut is lacking. By using a murine model of bacteria-induced typhlocolitis, we investigated the role of IL-17A, IL-17F, and IL-22 in cecal versus colonic inflammation. Cecal, but not colonic, pathology in C57BL/6 mice inoculated with Helicobacter hepaticus plus anti-IL-10 receptor (IL-10R) monoclonal antibody was exacerbated by co-administration of anti-IL-17A monoclonal antibody, suggesting a disease-protective role for IL-17A in the cecum...
December 2015: American Journal of Pathology
Avijit Ray, Sreemanti Basu, Raad Z Gharaibeh, Lydia C Cook, Ranjit Kumar, Elliot J Lefkowitz, Catherine R Walker, Casey D Morrow, Craig L Franklin, Terrence L Geiger, Nita H Salzman, Anthony Fodor, Bonnie N Dittel
It is clear that IL-10 plays an essential role in maintaining homeostasis in the gut in response to the microbiome. However, it is unknown whether IL-10 also facilitates immune homeostasis at distal sites. To address this question, we asked whether splenic immune populations were altered in IL-10-deficient (Il10(-/-)) mice in which differences in animal husbandry history were associated with susceptibility to spontaneous enterocolitis that is microbiome dependent. The susceptible mice exhibited a significant increase in splenic macrophages, neutrophils, and marginal zone (MZ) B cells that was inhibited by IL-10 signaling in myeloid, but not B cells...
October 1, 2015: Journal of Immunology: Official Journal of the American Association of Immunologists
Mark E Anderson, Emily C O'Brien, Emily N Grayek, James K Hermansen, Heather K Hunt
Current bacterial detection techniques are relatively slow, require bulky instrumentation, and usually require some form of specialized training. The gold standard for bacterial detection is culture testing, which can take several days to receive a viable result. Therefore, simpler detection techniques that are both fast and sensitive could greatly improve bacterial detection and identification. Here, we present a new method for the detection of the bacteria Helicobacter hepaticus using whispering-gallery mode (WGM) optical microcavity-based sensors...
2015: Biosensors
I C Arnold, S Mathisen, J Schulthess, C Danne, A N Hegazy, F Powrie
In inflammatory bowel diseases, a breakdown in host microbial interactions accompanies sustained activation of immune cells in the gut. Functional studies suggest a key role for interleukin-23 (IL-23) in orchestrating intestinal inflammation. IL-23 can be produced by various mononuclear phagocytes (MNPs) following acute microbial stimulation, but little is known about the key cellular sources of IL-23 that drive chronic intestinal inflammation. Here we have addressed this question using a physiological model of bacteria-driven colitis...
March 2016: Mucosal Immunology
Deepthi Nammi, Ravi C P K Srimath-Tirumala-Peddinti, Nageswara Rao R Neelapu
Helicobacter pylori colonize stomach, inducing gastritis, ulcers and gastric cancer. Drugs are used to relieve pain, but not H. pylori infections. Hence, there is a need for discovery of drug targets and drugs for H. pylori. An objective of this current study is to identify drug targets for H. pylori. RAST was used to compare genomes of 23 H. pylori strains with Homo sapiens sapiens, other Helicobacter species (H. acinonychis, H. hepaticus, H. mustalae) and among them, to identify 13471 unique genes. Bacterial genes which are non-homologous to humans and essential for pathogen are identified using BLASTp...
2016: Current Cancer Drug Targets
Irene Maier, Robert H Schiestl
Intestinal microbiota affect cell responses to ionizing radiation at the molecular level and can be linked to the development of the immune system, controlled cell death or apoptosis. We have developed a microbiota mouse model and report here that high-linear energy transfer (LET) radiation induced the repair of chromosomal DNA lesions more efficiently in conventional than in restricted intestinal microbiota mice. Based on different phylotype densities after whole-body irradiation, bacterial indicator phylotypes were found to be more abundant in restricted in microbiota than in conventional microbiota...
June 2015: Radiation Research
Jessica R Lakritz, Theofilos Poutahidis, Sheyla Mirabal, Bernard J Varian, Tatiana Levkovich, Yassin M Ibrahim, Jerrold M Ward, Ellen C Teng, Brett Fisher, Nicola Parry, Stephanie Lesage, Natalie Alberg, Sravya Gourishetti, James G Fox, Zhongming Ge, Susan E Erdman
Recent studies suggest that gastrointestinal tract microbiota modulate cancer development in distant non-intestinal tissues. Here we tested mechanistic hypotheses using a targeted pathogenic gut microbial infection animal model with a predilection to breast cancer. FVB-Tg(C3-1-TAg)cJeg/JegJ female mice were infected by gastric gavage with Helicobacter hepaticus at three-months-of-age putting them at increased risk for mammary tumor development. Tumorigenesis was multifocal and characterized by extensive infiltrates of myeloperoxidase-positive neutrophils otherwise implicated in cancer progression in humans and animal models...
April 20, 2015: Oncotarget
Isabelle C Arnold, Claire Hutchings, Ivanela Kondova, Ariann Hey, Fiona Powrie, Peter Beverley, Elma Tchilian
BCG, the only licensed vaccine against tuberculosis (TB), provides geographically variable protection, an effect ascribed to exposure to environmental mycobacteria (EM). Here we show that altering the intestinal microbiota of mice by early-life infection with the commensal bacterium Helicobacter hepaticus (Hh) increases their susceptibility to challenge with Mycobacterium tuberculosis (Mtb). Furthermore Hh-infected mice immunised parenterally with the recombinant subunit vaccine, human adenovirus type 5 expressing the immunodominant antigen 85A of Mtb (Ad85A), display a reduced lung immune response and protection against Mtb challenge is also reduced...
April 8, 2015: Vaccine
Cynthia G Alvarado, Andrew G Kocsis, Marcia L Hart, Marcus J Crim, Matthew H Myles, Craig L Franklin
Helicobacter spp. are some of the most prevalent bacterial contaminants of laboratory mice. Although abundant data regarding the diseases associated with H. hepaticus infection are available, little is known about the pathogenicity of H. ganmani, which was first isolated in 2001 from the intestines of laboratory mice. The objective of this study was to evaluate the host response to H. ganmani colonization in H. hepaticus disease-resistant C57BL/6 and disease-susceptible A/J and IL10-deficient mice. Mice were inoculated with H...
February 2015: Comparative Medicine
Fany Karina Segura-López, Alfredo Güitrón-Cantú, Javier Torres
Hepatobiliary cancers are highly lethal cancers that comprise a spectrum of invasive carcinomas originating in the liver hepatocellular carcinoma, the bile ducts intrahepatic cholangiocarcinoma and extrahepatic cholangiocarcinoma, the gallbladder and the ampulla of Vater (collectively known as biliary tract cancers). These tumors account for approximately 13% of all annual cancer-related deaths worldwide and for 10%-20% of deaths from hepatobiliary malignancies. Cholangiocarcinoma (CCA) is a devastating disease that displays a poor survival rate for which few therapeutic options are available...
February 7, 2015: World Journal of Gastroenterology: WJG
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