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Saulo de Tarso de Sá Pereira Segundo, Edgar Santiago Valesin, Mario Lenza, Durval do Carmo Barros Santos, Laercio Alberto Rosemberg, Mario Ferretti
Objective: To measure the interobserver reproducibility of the radiographic evaluation of lumbar spine instability. Methods: Measurements of the dynamic radiographs of the lumbar spine in lateral view were performed, evaluating the anterior translation and the angulation among the vertebral bodies. The tests were evaluated at workstations of the organization, through the Carestream Health Vue RIS (PACS), version Inc. 2009© system. Results: Agreement in detecting cases of radiographic instability among the observers varied from 88...
July 2016: Einstein
Rafael José Marques Peixoto, Karla Rodrigues Miranda, Leandro Araujo Lobo, Alessandra Granato, Pedro de Carvalho Maalouf, Hugo Emiliano de Jesus, Caio T C C Rachid, Saulo Roni Moraes, Henrique Fragoso Dos Santos, Raquel Silva Peixoto, Alexandre Soares Rosado, Regina Maria Cavalcanti Pilotto Domingues
The Antarctic soil microbial community has a crucial role in the growth and stabilization of higher organisms, such as vascular plants. Analysis of the soil microbiota composition in that extreme environmental condition is crucial to understand the ecological importance and biotechnological potential. We evaluated the efficiency of isolation and abundance of strict anaerobes in the vascular plant Deschampsia antarctica rhizosphere collected in the Antarctic's Admiralty Bay and associated biodiversity to metabolic perspective and enzymatic activity...
October 5, 2016: Extremophiles: Life Under Extreme Conditions
Saulo Pamato, Accácio-Lins do Valle, Gustavo-Henrique-Barbosa de Andrade, Hugo-Alberto Vidotti, Marcus-Vinícius-Reis Só, Jefferson-Ricardo Pereira
BACKGROUND: Evaluate the influence of different hybridization bonding techniques of a self-adhesive resin cement. MATERIAL AND METHODS: 30 human health molars were divided into six groups (n=10). The specimens received three longitudinal sections, allowing insertion of central cuts in PVC matrices. Each group received a different dentin pretreatment according to the manufacturer's recommendations, except the control group (G1), as follows. G2 - a 3-step total-etch adhesive system (Optibond™ FL, Kerr); G3 - a 3-step total-etch adhesive system (Adper™ Scotchbond™ Multi-Purpose, 3M ESPE); G4 - a 2-step total-etch adhesive system (Adper™ Single Bond 2, 3M ESPE); G5 - a single-step self-etching system (Bond Force, Tokuyama); and G6 - universal bonding system (Single Bond Universal, 3M ESPE)...
October 2016: Journal of Clinical and Experimental Dentistry
Tinde R van Andel, Rachel S Meyer, Saulo A Aflitos, Judith A Carney, Margaretha A Veltman, Dario Copetti, Jonathan M Flowers, Reinout M Havinga, Harro Maat, Michael D Purugganan, Rod A Wing, M Eric Schranz
African rice (Oryza glaberrima) and African cultivation practices are said to have influenced emerging colonial plantation economies in the Americas(1,2). However, the level of impact of African rice practices is difficult to establish because of limited written or botanical records(2,3). Recent findings of O. glaberrima in rice fields of Suriname Maroons bear evidence of the high level of knowledge about rice among African slaves and their descendants, who consecrate it in ancestor rituals(4,5). Here we establish the strong similarity, and hence likely origin, of the first extant New World landrace of O...
October 3, 2016: Nature Plants
Bruno L Anjos, Paulo V Peixoto, Saulo A Caldas, Daniel Bhaltazar, Ticiana N França, Aníbal G Armién
Plant intoxications in wildlife are difficult to diagnose, are overlooked, or are sometimes even neglected. Hence, factors that induce wild animals to ingest poisonous plants have not been sufficiently documented. An outbreak of glycoprotein storage disease in sambar deer ( Cervus unicolor ), induced by ingestion of the swainsonine-containing plant, common wireweed (Sida carpinifolia), is reported. Nine out of 55 deer held by a zoo in Brazil were affected. The poisoning was characterized by emaciation and neurologic signs followed by unexpected death in some of the animals...
September 2016: Journal of Zoo and Wildlife Medicine: Official Publication of the American Association of Zoo Veterinarians
Dermot O'Rourke, Saulo Martelli, Murk Bottema, Mark Taylor
Understanding the sensitivity of FE models to assumptions and uncertainties in geometric parameters and material properties is a fundamental step in understanding the reliability of model predictions. The computational cost of individual simulations and the large number of required models limits comprehensive quantification of model sensitivity. To quickly assess the sensitivity of a FE model, we built linear and Kriging surrogate models of a FE model of the intact hemipelvis. The percentage of the total sum of squares (%TSS) was used to determine the most influential input parameters and their possible interactions on the median, 95th percentile and maximum equivalent strains...
September 29, 2016: Journal of Biomechanical Engineering
Saulo Henrique Pires de Oliveira, Jiye Shi, Charlotte M Deane
MOTIVATION: Co-evolution methods have been used as contact predictors to identify pairs of residues that share spatial proximity. Such contact predictors have been compared in terms of the precision of their predictions, but there is no study that compares their usefulness to model generation. RESULTS: We compared eight different co-evolution methods for a set of ~3,500 proteins and found that metaPSICOV stage 2 produces, on average, the most precise predictions...
September 27, 2016: Bioinformatics
Michele Munk, Luiz O Ladeira, Bruno C Carvalho, Luiz S A Camargo, Nádia R B Raposo, Raquel V Serapião, Carolina C R Quintão, Saulo R Silva, Jaqueline S Soares, Ado Jorio, Humberto M Brandão
The pellucid zone (PZ) is a protective embryonic cells barrier against chemical, physical or biological substances. This put, usual transfection methods are not efficient for mammal oocytes and embryos as they are exclusively for somatic cells. Carbon nanotubes have emerged as a new method for gene delivery, and they can be an alternative for embryos transfection, however its ability to cross the PZ and mediated gene transfer is unknown. Our data confirm that multiwall carbon nanotubes (MWNTs) can cross the PZ and delivery of pDNA into in vitro-fertilized bovine embryos...
2016: Scientific Reports
Giuliano Lamberto, Saulo Martelli, Aurelio Cappozzo, Claudia Mazzà
Musculoskeletal models are widely used to estimate joint kinematics, intersegmental loads, and muscle and joint contact forces during movement. These estimates can be heavily affected by the soft tissue artefact (STA) when input positional data are obtained using stereophotogrammetry, but this aspect has not yet been fully characterised for muscle and joint forces. This study aims to assess the sensitivity to the STA of three open-source musculoskeletal models, implemented in OpenSim. A baseline dataset of marker trajectories was created for each model from experimental data of one healthy volunteer...
August 24, 2016: Journal of Biomechanics
T Portolés, C Sales, M Abalos, J Sauló, E Abad
The use of the novel atmospheric pressure chemical ionization (APCI) source for gas chromatography (GC) coupled to triple quadrupole using tandem mass spectrometry (MS/MS) and its potential for the simultaneous determination of the 12 dioxin-like polychlorobiphenyls (DL-PCBs) in complex food and feed matrices has been evaluated. In first place, ionization and fragmentation behavior of DL-PCBs on the APCI source under charge transfer conditions has been studied followed by their fragmentation in the collision cell...
September 21, 2016: Analytica Chimica Acta
Keigo Murakami, Yasuhiro Nakamura, Saulo J A Felizola, Ryo Morimoto, Fumitoshi Satoh, Kentaro Takanami, Hideki Katakami, Seiichi Hirota, Yoshiyu Takeda, Makiko Meguro-Horike, Shin-Ichi Horike, Michiaki Unno, Hironobu Sasano
Solitary fibrous tumors occasionally present with hypoglycemia because of the excessive release of insulin-like growth factor II. We report the first case of pancreatic solitary fibrous tumor causing ectopic adrenocorticotropic hormone syndrome. An 82-year-old Japanese man presented with lower limb edema, uncontrolled hypertension, hypokalemia, and baseline hypercortisolism. Distal pancreatectomy was performed after the clinical diagnosis of a neuroendocrine tumor with ectopic secretion of adrenocorticotropic hormone...
November 15, 2016: Molecular and Cellular Endocrinology
Saulo Molina-Giraldo, José Luis Pérez-Olivo, José Luis Rojas Arias, Edgar Acuña, Diana Alfonso, Mortimer Arreaza, Marcela Buitrago Leal
OBJECTIVES: The purpose of this study was to establish intracranial translucency reference values in healthy fetuses from a Latin American population. METHODS: This work was a cross-sectional retrospective correlational study. A review of sonographic reports from women between gestational ages of 11 weeks and 13 weeks 6 days at 2 health institutes in Bogota, Colombia, whose fetuses had a crown-rump length of 45 to 84 mm was conducted between January 1, 2010, and December 31, 2012...
October 2016: Journal of Ultrasound in Medicine: Official Journal of the American Institute of Ultrasound in Medicine
Lincoln S Taniguchi, Ulysses S Torres, Saulo M Souza, Lucas R Torres, Giuseppe D'Ippolito
BACKGROUND: The most widely accepted computed tomography (CT) protocol for diagnosis of acute pyelonephritis (APN) includes at least a pre- and post-contrast scan, which may expose patients to higher doses of ionizing radiation. PURPOSE: To establish the accuracy, reproducibility, and degree of confidence in CT diagnosis of acute pyelonephritis (APN) and urolithiasis using only images obtained during the nephrographic phase. MATERIAL AND METHODS: A retrospective study of 100 consecutive patients (88 women; age range, 19-70 years) with clinical and laboratory suspicion of APN and who underwent triphasic abdominal CT scans (non-contrast, nephrographic, and excretory phases) was performed...
August 25, 2016: Acta Radiologica
Aryane C Reis, Ana Luiza Franco, Victória R Campos, Flávia R Souza, Cristiane Zorzatto, Lyderson F Viccini, Saulo M Sousa
Agapanthus (Agapanthaceae) has 10 species described. However, most taxonomists differ respect to this number because the great phenotypic plasticity of the species. The cytogenetic has been an important tool to aid the plant taxon identification, and to date, all taxa of Agapanthus L'Héritier studied cytologically, presented 2n = 30. Although the species possess large chromosomes, the group is karyologically little explored. This work aimed to increase the cytogenetic knowledge of Agapanthus africanus (L.) Hoffmanns by utilization of chromosome banding techniques with DAPI / CMA3 and Fluorescent in situ Hybridization (FISH)...
August 18, 2016: Anais da Academia Brasileira de Ciências
Jaqueline L Damasceno, Pollyanna F Oliveira, Mariza A Miranda, Luis F Leandro, Nathália O Acésio, Saulo D Ozelin, Jairo K Bastos, Denise C Tavares
Solanum cernuum Vell is a Brazilian shrub or small tree, restricted to Southeast states of the country. The leaves are commercialized as "panacéia" and indicated for the treatment of urinary disorders, gonorrhea, scabies, skin diseases and as desobstruent, diuretic and antiarrhythmic. The hydroalcholic extract is active in the treatment of gastric ulcer. The aim of this study was to evaluate the genotoxic and antigenotoxic potential of S. cernuum hydroalcoholic extract (SC) in Swiss mice by micronucleus and comet assays...
August 20, 2016: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
Ronaldo Kobal, Irineu Loturco, Renato Barroso, Saulo Gil, Rogério Cuniyochi, Carlos Ugrinowitsch, Hamilton Roschel, Valmor Tricoli
The combination of strength (ST) and plyometric training (PT) has been shown to be effective for improving sport specific performance. However, there is no consensus about the most effective way to combine these methods in the same training session, in order to produce greater improvements in neuromuscular performance of soccer players. Thus, the purpose of this study was to compare the effects of different combinations of ST and PT sequences on strength, jump, speed, and agility capacities of elite young soccer players...
August 18, 2016: Journal of Strength and Conditioning Research
Dennis Becker, Saulo Varela Della Giustina, Sara Rodriguez-Mozaz, Rob Schoevaart, Damià Barceló, Matthias de Cazes, Marie-Pierre Belleville, José Sanchez-Marcano, Jean de Gunzburg, Olivier Couillerot, Johannes Völker, Jörg Oehlmann, Martin Wagner
In this study, the performance of immobilised laccase (Trametes versicolor) was investigated in combination with the mediator syringaldehyde (SYR) in removing a mixture of 38 antibiotics in an enzymatic membrane reactor (EMR). Antibiotics were spiked in osmosed water at concentrations of 10μg·L(-1) each. Laccase without mediator did not reduce the load of antibiotics significantly. The addition of SYR enhanced the removal: out of the 38 antibiotics, 32 were degraded by >50% after 24h. In addition to chemical analysis, the samples' toxicity was evaluated in two bioassays (a growth inhibition assay and the Microtox assay)...
November 2016: Bioresource Technology
Valéria N Lima, Cícera D M Oliveira-Tintino, Enaide S Santos, Luís P Morais, Saulo R Tintino, Thiago S Freitas, Yuri S Geraldo, Raimundo L S Pereira, Rafael P Cruz, Irwin R A Menezes, Henrique D M Coutinho
The indiscriminate use of antimicrobial drugs has increased the spectrum of exposure of these organisms. In our studies, these phenolic compounds were evaluated: gallic acid, caffeic acid and pyrogallol. The antibacterial, antifungal and modulatory of antibiotic activities of these compounds were assayed using microdilution method of Minimum Inhibitory Concentration (MIC) to bacteria and Minimum Fungicide Concentration (MFC) to fungi. The modulation was made by comparisons of the MIC and MFC of the compounds alone and combined with drugs against bacteria and fungi respectively, using a sub-inhibitory concentration of 128 μg/mL of substances (MIC/8)...
October 2016: Microbial Pathogenesis
Guilherme Curty Lechuga, Júlio Cesar Borges, Claudia Magalhães Calvet, Humberto Pinheiro de Araújo, Aline Araujo Zuma, Samara Braga do Nascimento, Maria Cristina Machado Motta, Alice Maria Rolim Bernardino, Mirian Claudia de Souza Pereira, Saulo Cabral Bourguignon
Chagas disease is a neglected tropical disease caused by the flagellated protozoan Trypanosoma cruzi. The current drugs used to treat this disease have limited efficacy and produce severe side effects. Quinolines, nitrogen heterocycle compounds that form complexes with heme, have a broad spectrum of antiprotozoal activity and are a promising class of new compounds for Chagas disease chemotherapy. In this study, we evaluated the activity of a series of 4-arylaminoquinoline-3-carbonitrile derivatives against all forms of Trypanosoma cruzi in vitro...
July 14, 2016: International Journal for Parasitology, Drugs and Drug Resistance
Rhaine Borges Santos Pedreira, Saulo Vasconcelos Rocha, Clarice Alves Dos Santos, Lélia Renata Carneiro Vasconcelos, Martha Cerqueira Reis
OBJECTIVE: Assess the content validity of the Elderly Health Assessment Tool with low education. METHODS: The data collection instrument/questionnaire was prepared and submitted to an expert panel comprising four healthcare professionals experienced in research on epidemiology of aging. The experts were allowed to suggest item inclusion/exclusion and were asked to rate the ability of individual items in questionnaire blocks to encompass target dimensions as "not valid", "somewhat valid" or "valid", using an interval scale...
April 2016: Einstein
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