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https://www.readbyqxmd.com/read/29154223/mitochondrial-f1-atpase-extends-glycolysis-and-ph-decline-in-an-in-vitro-model
#1
Sulaiman K Matarneh, Mariane Beline, Saulo de Luz E Silva, Hao Shi, David E Gerrard
The experiment was conducted to identify the mitochondrial protein responsible for enhancing glycolytic flux. We hypothesized that mitochondrial F1-ATPase promotes ATP hydrolysis and thereby the flux through glycolysis. Porcine longissimus muscle mitochondria were incorporated into an in vitro system designed to recapitulate postmortem glycolysis with or without Na-azide to specifically inhibit the β-subunit of mitochondrial F1-ATPase that catalyzes ATP hydrolysis. Addition of mitochondria enhanced ATP hydrolysis, glycogen degradation, lactate accumulation, and pH decline in the in vitro system...
November 7, 2017: Meat Science
https://www.readbyqxmd.com/read/29152619/dentin-%C3%AE-tbs-and-hardness-of-bulk-fill-and-conventional-composites-placed-in-a-box-shaped-cavity-preparation
#2
Francisca Daniele Jardilino Silame, Gizele de Pádua Geraldeli, Mário Alexandre Coelho Sinhoreti, Fernanda de Carvalho Panzeri Pires-de-Souza, Jean-Francois Roulet, Saulo Geraldeli
PURPOSE: To compare the dentin microtensile bond strength (μTBS) and the Knoop hardness of bulk-fill and conventional restorative composites in box-shaped Class I cavities using different insertion techniques. MATERIALS AND METHODS: Forty box-shaped Class I preparatons 4 mm deep were performed in the pulp chamber of sound human third molars. The restorations were made using either a conventional microhybrid (Z250, 3M ESPE) or bulk-fill (Tetric EvoCeram Bulk-fill, TCBF) composite using two incremental thicknesses: 2 mm or 4 mm (n = 10)...
November 17, 2017: Journal of Adhesive Dentistry
https://www.readbyqxmd.com/read/29149864/kinetic-estimated-glomerular-filtration-rate-in-critically-ill-patients-beyond-the-acute-kidney-injury-severity-classification-system
#3
Flávio de Oliveira Marques, Saulo Aires Oliveira, Priscila Ferreira de Lima E Souza, Wandervânia Gomes Nojoza, Maiara da Silva Sena, Taynara Muniz Ferreira, Bruno Gabriele Costa, Alexandre Braga Libório
BACKGROUND: Although significant advances have been achieved in acute kidney injury (AKI) research following its classification, potential pitfalls can be identified in clinical practice. The nonsteady-state (kinetic) estimated glomerular filtration rate (KeGFR) could add clinical and prognostic information in critically ill patients beyond the current AKI classification system. METHODS: This was a retrospective analysis using data from the Multiparameter Intelligent Monitoring in Intensive Care II project...
November 18, 2017: Critical Care: the Official Journal of the Critical Care Forum
https://www.readbyqxmd.com/read/29136098/sequential-search-leads-to-faster-more-efficient-fragment-based-de-novo-protein-structure-prediction
#4
Saulo H P de Oliveira, Eleanor C Law, Jiye Shi, Charlotte M Deane
Motivation: Most current de novo structure prediction methods randomly sample protein conformations and thus require large amounts of computational resource. Here, we consider a sequential sampling strategy, building on ideas from recent experimental work which shows that many proteins fold cotranslationally. Results: We have investigated whether a pseudo-greedy search approach, which begins sequentially from one of the termini, can improve the performance and accuracy of de novo protein structure prediction...
November 9, 2017: Bioinformatics
https://www.readbyqxmd.com/read/29118633/checklist-of-the-freshwater-fishes-of-colombia-a-darwin-core-alternative-to-the-updating-problem
#5
Carlos DoNascimiento, Edgar Esteban Herrera-Collazos, Guido A Herrera-R, Armando Ortega-Lara, Francisco A Villa-Navarro, José Saulo Usma Oviedo, Javier A Maldonado-Ocampo
The present work is part of a process to create a Catalogue of the Freshwater Fishes of Colombia and consisted in the depuration and updating of the taxonomic and geographic components of the checklist of the freshwater fishes of Colombia. An exhaustive revision of the 1435 species recorded in 2008 was necessary to: 1. Add new species described since 2009 and species originally described from Colombia but inadvertently omitted in 2008; 2. Add new records of already described species; 3. Delete species whose presence in Colombia was not supported by voucher specimens in ichthyological collections; and 4...
2017: ZooKeys
https://www.readbyqxmd.com/read/29112282/antifungals-discovery-an-insight-into-new-strategies-to-combat-antifungal-resistance
#6
REVIEW
Alexandre M Fuentefria, Bruna Pippi, Daiane F Dalla Lana, Katarzyna K Donato, Saulo F de Andrade
Undeniably, new antifungal treatments are necessary against pathogenic fungi. Fungal infections have significantly increased in recent decades, being highlighted as important causes of morbidity and mortality, particularly in immunocompromised patients. Five main antifungal classes are used: (i) azoles, (ii) echinocandins, (iii) polyenes, (iv) allylamines and (v) pyrimidine analogs. Moreover, the treatment of mycoses has several limitations, such as; undesirable side effects, narrow activity spectrum, a small number of targets and fungal resistance; which are still of major concern in clinical practice...
November 7, 2017: Letters in Applied Microbiology
https://www.readbyqxmd.com/read/29100064/outbreak-of-chikungunya-virus-in-a-vulnerable-population-of-sergipe-brazil-a-molecular-and-serological-survey
#7
Marielton Dos Passos Cunha, Cliomar Alves Dos Santos, Daniel Ferreira de Lima Neto, Alessandra Soares Schanoski, Shahab Zaki Pour, Saulo Duarte Passos, Mércia Simone Feitosa de Souza, Danuza Duarte Costa, Paolo Marinho de Andrade Zanotto
BACKGROUND: Chikungunya virus (CHIKV) is a re-emerging arbovirus that is causing outbreaks in several countries of the Americas. The virus was introduced in Brazil in 2014, and since then, several Brazilian states have notified autochthonous cases. OBJECTIVES: Provide additional evidence on a CHIKV outbreak and an outline of the laboratory and clinical profile of symptomatic patients in Sergipe, Brazil. STUDY DESIGN: In February 2016, we collected 142 serum samples from symptomatic patients for arboviruses in Sergipe, Brazil...
October 31, 2017: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/29093309/urethral-prolapse-corrected-by-microsurgery-in-a-cat-a-case-report
#8
Luciana Gonçalves Teixeira, Michelli Westphal De Ataide, Vanessa Milech, Felipe Baldissarella Gavioli, Bernardo Nascimento Antunes, Stephanie Lanzarini Abati, Mauricio Veloso Brun, Saulo Tadeu Lemos Pinto Filho
Urethral prolapse is a rare condition in dogs and cats. A 7-month-old male cat presented with intermittent penile bleeding and a red mass protruding from the tip of the penis. Urethral prolapse was diagnosed by using a surgical microcamera to examine the genital area. Urethroplasty and preputial advancement surgery were performed using a surgical microscope, to successfully correct the urethral prolapse.
October 31, 2017: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/29090398/photobiomodulation-therapy-for-the-improvement-of-muscular-performance-and-reduction-of-muscular-fatigue-associated-with-exercise-in-healthy-people-a-systematic-review-and-meta-analysis
#9
REVIEW
Adriane Aver Vanin, Evert Verhagen, Saulo Delfino Barboza, Leonardo Oliveira Pena Costa, Ernesto Cesar Pinto Leal-Junior
Researches have been performed to investigate the effects of phototherapy on improving performance and reduction of muscular fatigue. However, a great variability in the light parameters and protocols of the trials are a concern to establish the efficacy of this therapy to be used in sports or clinic. The aim of this study is to investigate the effectiveness, moment of application of phototherapy within an exercise protocol, and which are the parameters optimally effective for the improvement of muscular performance and the reduction of muscular fatigue in healthy people...
October 31, 2017: Lasers in Medical Science
https://www.readbyqxmd.com/read/29072713/leisure-time-physical-inactivity-among-healthcare-workers
#10
Saulo Vasconcelos Rocha, Aline Rodrigues Barbosa, Tania Maria Araújo
OBJECTIVES: To estimate the prevalence of leisure-time physical inactivity (LTPI) and associated factors among healthcare workers. MATERIAL AND METHODS: The cross-sectional study carried out with 2684 healthcare workers from 4 municipalities from the northeast region, Brazil. The LTPI was assessed by dichotomous question. The association between LTPI and the various independent variables was examined through the multinomial logistic regression analysis (crude and adjusted)...
October 26, 2017: International Journal of Occupational Medicine and Environmental Health
https://www.readbyqxmd.com/read/29071115/acceptability-and-perceptions-of-end-users-towards-an-online-sports-health-surveillance-system
#11
Saulo Delfino Barboza, Caroline Silveira Bolling, Joske Nauta, Willem van Mechelen, Evert Verhagen
AIM: To describe the acceptability and the perceptions of athletes and staff members (ie, end-users) towards an online sports-health surveillance system. METHODS: A pilot study with a mixed-methods approach was pursued. Descriptive analysis was conducted to present the adherence of judo (n=34), swimming (n=21) and volleyball (n=14) athletes to an online registration of their sport exposure and any health complaints between April 2014 and January 2015. End-users' perceptions towards the system were investigated qualitatively with semistructured interviews (n=21)...
2017: BMJ Open Sport & Exercise Medicine
https://www.readbyqxmd.com/read/29050543/granulomatous-necrotizing-myositis-in-swine-affected-by-porcine-circovirus-disease
#12
Guilherme Konradt, Raquel A S Cruz, Daniele M Bassuino, Matheus V Bianchi, Caroline P de Andrade, Fernando S da Silva, David Driemeier, Saulo P Pavarini
Porcine circovirus type 2 (PCV2) is associated with multiple clinical syndromes in pigs, known as porcine circovirus diseases. This work describes an outbreak of porcine circovirus diseases with severe lesions affecting the skeletal muscle. Ninety-two pigs had apathy, weight loss, and diarrhea over a clinical course of 7 to 10 days. Approximately 30 of the pigs had stiff gait, muscle weakness, hind limb paresis, and recumbency. Twelve of the 92 pigs were necropsied, and 4 had pale discoloration of skeletal muscles with microscopic lesions of granulomatous necrotizing myositis...
January 1, 2017: Veterinary Pathology
https://www.readbyqxmd.com/read/29043920/simultaneity-of-leisure-behaviors-and-common-mental-disorders-among-healthcare-workers
#13
Saulo Vasconcelos Rocha, Aline Rodrigues Barbosa, Tania Maria de Araújo
OBJECTIVE: To investigate the aggregation of four leisure behaviors and the association between the number of leisure behaviors and common mental disorders (CMD). METHODS: A cross-sectional study was carried out with a representative sample of healthcare workers of Brazil. The aggregation of the types of leisure activities was assessed by comparing the prevalence observed with the expected prevalence. The logistic regression analysis evaluated the association between the number of leisure behaviors and CMD...
October 18, 2017: Archives of Environmental & Occupational Health
https://www.readbyqxmd.com/read/29041860/maternal-behavior-of-the-mouse-dam-toward-pups-implications-for-maternal-separation-model-of-early-life-stress
#14
Rodrigo Orso, Luis Eduardo Wearick-Silva, Kerstin Camile Creutzberg, Anderson Centeno-Silva, Laura Glusman Roithmann, Rafaelly Pazzin, Saulo Gantes Tractenberg, Fernando Benetti, Rodrigo Grassi-Oliveira
Maternal care is essential for an adequate pup development, as well as for the health of the dam. Exposure to stress in early stages of life can disrupt this dam-pup relationship promoting altered neurobiological and behavioral phenotypes. However, there is a lack of consensus regarding the effects of daily maternal separation (MS) on the pattern of maternal behavior. The aim of this study is to compare the patterns of maternal behavior between mice exposed to MS and controls. BALB/c mice were subjected to MS for a period of 180 min/day from postnatal day 2-7 (n = 17) or designated to be standard animal facility reared (AFR) controls (n = 19)...
October 18, 2017: Stress: the International Journal on the Biology of Stress
https://www.readbyqxmd.com/read/28990013/primary-oral-melanoma-a-clinicopathologic-review-and-case-presentation
#15
Mayara Santos de Castro, Bruno Saulo de Assis Reis, Denismar Alves Nogueira, Marina Lara de Carli, João Adolfo Costa Hanemann, Alessandro Antônio Costa Pereira, Oslei Paes de Almeida, Felipe Fornias Sperandio
OBJECTIVE: The purpose of this manuscript is to present a clinicopathologic review of the literature concerning all the detailed cases of primary oral melanoma (OM) that were confirmed by immunohistochemistry. In addition, a pertinent case presentation is addressed. DATA SOURCES: An extensive electronic search of the literature was performed using PubMed/Medline from 1953 to 2017. Relevant articles were selected based on specific inclusion criteria. Statistical analyses were conducted by the Shapiro-Wilk, Fisher's exact, chi-square, and Z tests (α = ...
October 6, 2017: Quintessence International
https://www.readbyqxmd.com/read/28989902/quantitative-assessment-of-image-artifacts-from-root-filling-materials-on-cbct-scans-made-using-several-exposure-parameters
#16
Katharina Alves Rabelo, Yuri Wanderley Cavalcanti, Martina Gerlane de Oliveira Pinto, Saulo Leonardo Sousa Melo, Paulo Sérgio Flores Campos, Luciana Soares de Andrade Freitas Oliveira, Daniela Pita de Melo
PURPOSE: To quantify artifacts from different root filling materials in cone-beam computed tomography (CBCT) images acquired using different exposure parameters. MATERIALS AND METHODS: Fifteen single-rooted teeth were scanned using 8 different exposure protocols with 3 different filling materials and once without filling material as a control group. Artifact quantification was performed by a trained observer who made measurements in the central axial slice of all acquired images in a fixed region of interest using ImageJ...
September 2017: Imaging Science in Dentistry
https://www.readbyqxmd.com/read/28978781/crystal-structure-of-pharmaceutical-cocrystals-of-2-6-diaminopyridine-with-piracetam-and-theophylline
#17
Melissa Hidekel Durán-Palma, Sonia Sanet Mendoza-Barraza, Nancy Evelyn Magaña-Vergara, Francisco Javier Martínez-Martínez, Juan Saulo González-González
Pharmaceutical cocrystals are crystalline solids formed by an active pharmaceutical ingredient and a cocrystal former. The cocrystals 2,6-diaminopyridine (DAP)-piracetam [PIR; systematic name: 2-(2-oxopyrrolidin-1-yl)acetamide] (1/1), C5H7N3·C6H10N2O2, (I), and 2,6-diaminopyridine-theophylline (TEO; systematic name: 1,3-dimethyl-7H-purine-2,6-dione) (1/1), C5H7N3·C7H8N4O2, (II), were prepared by the solvent-assisted grinding method and were characterized by IR spectroscopy and powder X-ray diffraction. Cocrystal (I) crystallized in the orthorhombic space group Pbca and showed a 1:1 stoichiometry...
October 1, 2017: Acta Crystallographica. Section C, Structural Chemistry
https://www.readbyqxmd.com/read/28965247/doxycycline-loaded-nanotube-modified-adhesives-inhibit-mmp-in-a-dose-dependent-fashion
#18
Jadesada Palasuk, L Jack Windsor, Jeffrey A Platt, Yuri Lvov, Saulo Geraldeli, Marco C Bottino
OBJECTIVES: This article evaluated the drug loading, release kinetics, and matrix metalloproteinase (MMP) inhibition of doxycycline (DOX) released from DOX-loaded nanotube-modified adhesives. DOX was chosen as the model drug, since it is the only MMP inhibitor approved by the U.S. Food and Drug Administration. MATERIALS AND METHODS: Drug loading into the nanotubes was accomplished using DOX solution at distinct concentrations. Increased concentrations of DOX significantly improved the amount of loaded DOX...
September 30, 2017: Clinical Oral Investigations
https://www.readbyqxmd.com/read/28953138/local-flap-technique-after-removing-premaxilla-for-closure-of-oronasal-fistula
#19
Ighor Andrade Fernandes, Dhelfeson Willya Douglas-de-Oliveira, Saulo Gabriel Moreira Falci, Cássio Roberto Rocha Dos Santos
This letter aimed to describe a surgical technique for oronasal fistula closure that promotes efficient healing and minimizes recurrence. The oronasal fistula was repaired by buccal flap. The technique proved to be simple and efficient.
November 2017: Journal of Craniofacial Surgery
https://www.readbyqxmd.com/read/28951306/anti-platelet-aggregation-activity-of-two-novel-acidic-asp49-phospholipases-a2-from-bothrops-brazili-snake-venom
#20
Juliana C Sobrinho, Anderson M Kayano, Rodrigo Simões-Silva, Jorge J Alfonso, Ana F Gomez, Maria C Vega Gomez, Fernando B Zanchi, Laura A Moura, Vivian R Souza, André L Fuly, Eliandre de Oliveira, Saulo L da Silva, José R Almeida, Juliana P Zuliani, Andreimar M Soares
Phospholipases A2 (PLA2s) are important enzymes present in snake venoms and are related to a wide spectrum of pharmacological effects, however the toxic potential and therapeutic effects of acidic isoforms have not been fully explored and understood. Due to this, the present study describes the isolation and biochemical characterization of two new acidic Asp49-PLA2s from Bothrops brazili snake venom, named Braziliase-I and Braziliase-II. The venom was fractionated in three chromatographic steps: ion exchange, hydrophobic interaction and reversed phase...
September 23, 2017: International Journal of Biological Macromolecules
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