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Clara Bodelon, Hannah Oh, Nilanjan Chatterjee, Montserrat Garcia-Closas, Maya Palakal, Mark E Sherman, Ruth M Pfeiffer, Berta M Geller, Pamela M Vacek, Donald L Weaver, Rachael E Chicoine, Daphne Papathomas, Jackie Xiang, Deesha A Patel, Zeina G Khodr, Laura Linville, Susan E Clare, Daniel W Visscher, Carolyn Mies, Stephen M Hewitt, Louise A Brinton, Anna Maria Storniolo, Chunyan He, Stephen J Chanock, Gretchen L Gierach, Jonine D Figueroa
Terminal duct lobular units (TDLUs) are the predominant source of future breast cancers, and lack of TDLU involution (higher TDLU counts, higher acini count per TDLU and the product of the two) is a breast cancer risk factor. Numerous breast cancer susceptibility single nucleotide polymorphisms (SNPs) have been identified, but whether they are associated with TDLU involution is unknown. In a pooled analysis of 872 women from two studies, we investigated 62 established breast cancer SNPs and relationships with TDLU involution...
February 15, 2017: International Journal of Cancer. Journal International du Cancer
Jolanta M Topczewska, Ramy A Shoela, Joanna P Tomaszewski, Rupa B Mirmira, Arun K Gosain
Using morphological, histological, and TEM analyses of the cranium, we provide a detailed description of bone and suture growth in zebrafish. Based on expression patterns and localization, we identified osteoblasts at different degrees of maturation. Our data confirm that, unlike in humans, zebrafish cranial sutures maintain lifelong patency to sustain skull growth. The cranial vault develops in a coordinated manner resulting in a structure that protects the brain. The zebrafish cranial roof parallels that of higher vertebrates and contains five major bones: one pair of frontal bones, one pair of parietal bones, and the supraoccipital bone...
2016: PloS One
Yang Li, Xu-Qing Tang, Zhonghu Bai, Xiaofeng Dai
Exploring the intrinsic differences among breast cancer subtypes is of crucial importance for precise diagnosis and therapeutic decision-making in diseases of high heterogeneity. The subtypes defined with several layers of information are related but not consistent, especially using immunohistochemistry markers and gene expression profiling. Here, we explored the intrinsic differences among the subtypes defined by the estrogen receptor, progesterone receptor and human epidermal growth factor receptor 2 based on the decision tree...
October 27, 2016: Scientific Reports
Xiang Zhang, Lijuan Wang, Yingmei Wang, Shenjia Shi, Huayu Zhu, Fengjin Xiao, Jing Yang, Angang Yang, Xiaoke Hao
Forkhead box Q1 (FOXQ1) has been recognized as an oncogene that is overexpressed in different cancers, and several studies have shown that FOXQ1 is related to apoptosis and proliferation in many cancer types. However, the role and the molecular mechanism of FOXQ1 in prostate cancer remains unclear. In this study, we aimed to explore the role of FOXQ1 in regulating cell apoptosis, proliferation and invasion in prostate cancer and the underlying mechanism. We found that FOXQ1 was highly expressed in the prostate cancer tissues and cell lines...
October 2016: Oncology Reports
Hock Chuan Yeo, Sherwin Ting, Romulo Martin Brena, Geoffrey Koh, Allen Chen, Siew Qi Toh, Yu Ming Lim, Steve Kah Weng Oh, Dong-Yup Lee
The differentiation efficiency of human embryonic stem cells (hESCs) into heart muscle cells (cardiomyocytes) is highly sensitive to culture conditions. To elucidate the regulatory mechanisms involved, we investigated hESCs grown on three distinct culture platforms: feeder-free Matrigel, mouse embryonic fibroblast feeders, and Matrigel replated on feeders. At the outset, we profiled and quantified their differentiation efficiency, transcriptome, transcription factor binding sites and DNA-methylation. Subsequent genome-wide analyses allowed us to reconstruct the relevant interactome, thereby forming the regulatory basis for implicating the contrasting differentiation efficiency of the culture conditions...
2016: Scientific Reports
Ying Cui, Aijun Qiao, Tao Jiao, Huabing Zhang, Yuan Xue, Yongkang Zou, Anfang Cui, Fude Fang, Yongsheng Chang
AIM/HYPOTHESIS: Hepatic forkhead box q1 (FOXQ1) expression levels are regulated by nutritional and pathophysiological status. In this study we investigated the role of FOXQ1 in the regulation of hepatic gluconeogenesis. METHODS: We used multiple mouse and cell models to study the role of FOXQ1 in regulating expression of gluconeogenic genes, and cellular and hepatic glucose production. RESULTS: Expression of hepatic FOXQ1 was regulated by fasting in normal mice and was dysregulated in diabetic mice...
October 2016: Diabetologia
LingYun Yang, Ke Yi, HongJing Wang, YiQi Zhao, MingRong Xi
Long non-coding RNAs are emerging to be novel regulators in gene expression. In current study, lncRNAs microarray and lncRNA-mRNA co-expression analysis were performed to explore the alternation and function of lncRNAs in cervical cancer cells. We identified that 4750 lncRNAs (15.52%) were differentially expressed in SiHa (HPV-16 positive) (2127 up-regulated and 2623 down-regulated) compared with C-33A (HPV negative), while 5026 lncRNAs (16.43%) were differentially expressed in HeLa (HPV-18 positive) (2218 up-regulated and 2808 down-regulated) respectively...
August 2, 2016: Oncotarget
Anja Fleger-Weckmann, Yasemin Üstün, Jennifer Kloepper, Ralf Paus, Wilhelm Bloch, Zu-Lin Chen, Jeannine Wegner, Lydia Sorokin, Lutz Langbein, Beate Eckes, Paola Zigrino, Thomas Krieg, Roswitha Nischt
Laminins are the most abundant non-collagenous basement membrane (BM) components, composed of an α, β and γ chain. The laminin γ1 chain, encoded by LAMC1, is the most abundant γ chain. The main laminin isoforms in the dermo-epidermal junction (DEJ) are laminin-332, laminin-511 and laminin-211, the latter being restricted to the lower part of hair follicles (HFs). Complete deletion of LAMC1 results in lethality around embryonic day 5.5. To study the function of laminin γ1 containing isoforms in skin development and maturation after birth, we generated mice lacking LAMC1 expression in basal keratinocytes (LAMC1(EKO)) using the keratin 14 (K14) Cre/loxP system...
December 2016: Matrix Biology: Journal of the International Society for Matrix Biology
Yang Li, Yefei Zhang, Zhendong Yao, Sisi Li, Zhenhua Yin, Min Xu
As a member of the Forkhead box protein family, Forkhead box Q1 (FOXQ1) is a transcription factor that functions to regulate cell differentiation. Recently, an increasing number of studies have demonstrated that FOXQ1 is significantly associated with the pathogenesis of tumors. This review aims to predominantly discuss the relationship between FOXQ1 and various types of tumor. The FOXQ1 gene is located at human chromosome 6p25.3 and encodes a functional 403 amino acid protein, which has many physiological functions, including promoting epithelial differentiation, inhibiting smooth muscle differentiation, activating T cells and autoimmunity, and controlling mucin gene expression and granule content in stomach surface mucous cells...
July 2016: International Journal of Oncology
Wenhao Weng, Yoshinaga Okugawa, Shusuke Toden, Yuji Toiyama, Masato Kusunoki, Ajay Goel
PURPOSE: Colorectal cancer ranks as the third most frequent cancer type, and its incidence continues to rise gradually worldwide, highlighting the need to identify previously unrecognized molecular events that propel development of this malignancy. Recent evidence suggests that dysregulated expression of FOX family of transcription factors may be critical in various genetic disorders as well as cancer; however, the functional and clinical significance of this pathway in colorectal cancer remains unclear...
October 1, 2016: Clinical Cancer Research: An Official Journal of the American Association for Cancer Research
Su-Hyeong Kim, Catherine H Kaschula, Nolan Priedigkeit, Adrian V Lee, Shivendra V Singh
Diallyl trisulfide (DATS), a metabolic byproduct of garlic, is known to inhibit the growth of breast cancer cells in vitro and in vivo This study demonstrates that DATS targets breast cancer stem cells (bCSC). Exposure of MCF-7 and SUM159 human breast cancer cells to pharmacological concentrations of DATS (2.5 and 5 μm) resulted in dose-dependent inhibition of bCSC, as evidenced by a mammosphere assay and flow cytometric analysis of aldehyde dehydrogenase 1 (ALDH1) activity and the CD44(high)/CD24(low)/epithelial specific antigen-positive fraction...
June 24, 2016: Journal of Biological Chemistry
Radhakrishnan Vishnubalaji, Rimi Hamam, Shijun Yue, Omar Al-Obeed, Moustapha Kassem, Fei-Fei Liu, Abdullah Aldahmash, Nehad M Alajez
Colorectal cancer (CRC) is the third most common cancer causing high mortality rates world-wide. Delineating the molecular mechanisms leading to CRC development and progression, including the role of microRNAs (miRNAs), are currently being unravelled at a rapid rate. Here, we report frequent downregulation of the microRNA miR-320 family in primary CRC tissues and cell lines. Lentiviral-mediated re-expression of miR-320c (representative member of the miR-320 family) inhibited HCT116 CRC growth and migration in vitro, sensitized CRC cells to 5-Fluorouracil (5-FU), and inhibited tumor formation in SCID mice...
June 14, 2016: Oncotarget
Jing Zhang, Yimin Liu, Jia Zhang, Xiaohai Cui, Gang Li, Jiansheng Wang, Hong Ren, Yunfeng Zhang
Gastric cancer (GC) is one of the most common cancers, and the second most common cause of cancer deaths worldwide. Forkhead box Q1 (FOXQ1) is a member of the forkhead transcription factor family and its upregulation is closely correlated with tumor progression and prognosis of multiple cancer types, including GC. FOXQ1 has been shown to regulate EMT and function in human cancers. However, the role of FOXQ1 in regulating EMT in GC and the exactly mechanism has not been clarified. The purpose of this study was to investigate the effects of FOXQ1 on EMT in human GC...
June 2016: Oncology Reports
K Hardy, F Wu, W Tu, A Zafar, T Boulding, R McCuaig, C R Sutton, A Theodoratos, S Rao
Epithelial-to-mesenchymal transition (EMT) is physiological in embryogenesis and wound healing but also associated with the formation of cancer stem cells (CSCs). Many EMT signaling pathways are implicated in CSC formation, but the precise underlying mechanisms of CSC formation remain elusive. We have previously demonstrated that PKC is critical for EMT induction and CSC formation in inducible breast EMT/CSC models. Here, we used formaldehyde-assisted isolation of regulatory elements-sequencing (FAIRE-seq) to investigate DNA accessibility changes after PKC activation and determine how they influence EMT and CSC formation...
2016: Nucleus
Mingting Zhang, Qingli Xu, Shufen Yan, Zhigang Li, Wei Yan, Xiaojing Jia
MicroRNAs (miRNAs) play a pivotal role in cancer progression and development, representing novel therapeutic tools for cancer therapy. Forkhead box Q1 (FOXQ1) functions as an oncogene in various cancer types. However, the functional significance of FOXQ1 in cervical cancer remains unknown. In this study, we investigated the biological function of FOXQ1 in cervical cancer and tested whether or not FOXQ1 can be targeted and regulated by specific miRNAs. We found that FOXQ1 was highly expressed in cervical cancer cell lines...
May 2016: Oncology Reports
Bo Xiao, Huazhen Liu, Zeyun Gu, Cheng Ji
INTRODUCTION: MicroRNA (miR) was implicated in the tumorigenesis of many types of cancer, but no study was conducted on the exact role of miR-133 in lung cancer. METHODS: We have identified miR-133 as a putative regulator of FOXQ1 expression, and investigated the potential involvement of miR-133 in the migration and invasion of lung cancer cells, as well as the underlying molecular mechanism. RESULTS: MiR-133 directly targeted and down-regulated FOXQ1 expression, which in turn reduced TGF-β level...
October 2016: Archivos de Bronconeumología
Alfonso Bettin, Ismael Reyes, Niradiz Reyes
BACKGROUND: The aim of this study was to evaluate the gene expression profiles of a set of prostate cancer-associated genes in prostate cancer cell lines, to determine their association with different cancer phenotypes and identify potential novel biomarkers for this disease. METHODS: Quantitative real-time PCR was used to determine the expression profiles of 21 prostate cancer-associated genes in the human prostate cancer cell lines PC-3 and LNCaP, using the nontumorigenic cell line PWR-1E as control cell line...
May 28, 2016: International Journal of Biological Markers
Andrea de Faria F Belone, Patrícia S Rosa, Ana P F Trombone, Luciana R V Fachin, Cássio C Guidella, Somei Ura, Jaison A Barreto, Mabel G Pinilla, Alex F de Carvalho, Dirce M Carraro, Fernando A Soares, Cleverson T Soares
Leprosy, an infectious disease caused by Mycobacterium leprae, affects millions of people worldwide. However, little is known regarding its molecular pathophysiological mechanisms. In this study, a comprehensive assessment of human mRNA was performed on leprosy skin lesions by using DNA chip microarrays, which included the entire spectrum of the disease along with its reactional states. Sixty-six samples from leprotic lesions (10TT, 10BT, 10BB, 10BL, 4LL, 14R1, and 10R2) and nine skin biopsies from healthy individuals were used as controls (CC) (ages ranged from 06 to 83 years, 48 were male and 29 female)...
2015: Frontiers in Genetics
Jie Zhang, Wei Li, Song Dai, Xuhui Tai, Jianping Jia, Xing Guo
Forkhead box Q1 (FOXQ1) is a forkhead transcription factor that is involved in numerous biological processes and has been shown to participate in tumorigenesis. However, the clinical significance of the expression of this protein in laryngeal carcinoma, and the mechanisms underlying its regulation in this disease remain unclear. The aim of present study was to measure the expression of FOXQ1 in laryngeal carcinoma, and to examine its effect on tumorigenesis. In the present study, reverse transcription-quantitative polymerase chain reaction and western blotting were employed to measure FOXQ1 expression in laryngeal carcinoma tissue samples, small interfering RNA specific to FOXQ1, was transfected into Hep2 cells and its effect on cell proliferation, cell cycle progression and cell migration was examined, using a CCK-8 assay, flow cytometry and a transwell migration assay, respectively...
October 2015: Oncology Letters
Kathie-Anne Walters, Rachael Olsufka, Rolf E Kuestner, Xiagang Wu, Kai Wang, Shawn J Skerrett, Adrian Ozinsky
BACKGROUND: Francisella infection attenuates immune cell infiltration and expression of selected pro-inflammatory cytokines in response to endogenous LPS, suggesting the bacteria is actively antagonizing at least some part of the response to Toll-like receptor 4 (TLR4) engagement. The ability of different Francisella strains to inhibit the ability of E. coli LPS to induce a pulmonary inflammatory response, as measured by gene expression profiling, was examined to define the scope of modulation and identify of inflammatory genes/pathways that are specifically antagonized by a virulent F...
2015: BMC Genomics
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