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https://www.readbyqxmd.com/read/29249164/effect-of-river-water-exposition-on-adhesion-and-invasion-abilities-of-salmonella-oranienburg-and-saintpaul
#1
Mitzi Dayanira Estrada-Acosta, Karina Ramirez, José Andrés Medrano-Félix, Nohelia Castro-Del Campo, Hector S López-Moreno, Maribel Jimenez Edeza, Jaime Martínez-Urtaza, Cristóbal Chaidez
This study was performed to evaluate in vitro the adherence and invasiveness capacity of Salmonella Oranienburg and Saintpaul (isolated from river water) exposed to laboratory and river water growth conditions and inoculated into epithelial HEp-2 cell. Results showed that Salmonella Oranienburg and Salmonella Saintpaul showed lower ability to adhere and invade epithelial HEp-2 cells under both growth conditions as compared to Salmonella Typhimurium reference strain. S. Oranienburg adhesion capacity was not affected by the growth conditions, while S...
December 18, 2017: International Journal of Environmental Health Research
https://www.readbyqxmd.com/read/29221141/plasma-derived-extracellular-vesicle-proteins-as-a-source-of-biomarkers-for-lung-adenocarcinoma
#2
Jody Vykoukal, Nan Sun, Clemente Aguilar-Bonavides, Hiroyuki Katayama, Ichidai Tanaka, Johannes F Fahrmann, Michela Capello, Junya Fujimoto, Mitzi Aguilar, Ignacio I Wistuba, Ayumu Taguchi, Edwin J Ostrin, Samir M Hanash
Exosomes and other extracellular vesicles (EVs) have been implicated as mediators of intercellular communication. Their release into the circulation has the potential to inform about tumor status. In-depth proteomic characterization of plasma-derived EVs has been limited by challenges in isolating EVs from protein-abundant biological fluids. We implemented a novel single-step density gradient flotation workflow for efficient and rapid isolation of highly enriched circulating EVs from plasma. Mass-spectrometry analysis of plasma EVs from subjects with lung adenocarcinoma and matched controls resulted in the identification of 640 proteins...
November 10, 2017: Oncotarget
https://www.readbyqxmd.com/read/29187638/numb-prevents-a-complete-epithelial-mesenchymal-transition-by-modulating-notch-signalling
#3
Federico Bocci, Mohit K Jolly, Satyendra C Tripathi, Mitzi Aguilar, Samir M Hanash, Herbert Levine, José N Onuchic
Epithelial-mesenchymal transition (EMT) plays key roles during embryonic development, wound healing and cancer metastasis. Cells in a partial EMT or hybrid epithelial/mesenchymal (E/M) phenotype exhibit collective cell migration, forming clusters of circulating tumour cells-the primary drivers of metastasis. Activation of cell-cell signalling pathways such as Notch fosters a partial or complete EMT, yet the mechanisms enabling cluster formation remain poorly understood. Using an integrated computational-experimental approach, we examine the role of Numb-an inhibitor of Notch intercellular signalling-in mediating EMT and clusters formation...
November 2017: Journal of the Royal Society, Interface
https://www.readbyqxmd.com/read/29160493/neonatal-appendicitis-a-survival-case-study
#4
Izabela Linha Secco, Taine Costa, Etiene Letícia Leone de Moraes, Márcia Helena de Souza Freire, Mitzy Tannia Reichembach Danski, Regina Paula Guimarães Vieira Cavalcante
OBJECTIVE: To report a case of neonatal appendicitis in a children's hospital in southern Brazil, demonstrating the impact on neonatal survival. METHOD: Case study with data collection from medical records, approved by the Institution and Ethics Committee for Research with Human Beings. RESULTS: The clinical picture is initially characterized by food intolerance, evolving to hypoactivity, alteration of vital signs and septicemia due to intestinal perforation...
November 2017: Revista Brasileira de Enfermagem
https://www.readbyqxmd.com/read/29142285/blockade-of-cb1-cannabinoid-receptor-alters-gut-microbiota-and-attenuates-inflammation-and-diet-induced-obesity
#5
Pegah Mehrpouya-Bahrami, Kumaraswamy Naidu Chitrala, Mitra S Ganewatta, Chuanbing Tang, E Angela Murphy, Reilly T Enos, Kandy T Velazquez, Jamie McCellan, Mitzi Nagarkatti, Prakash Nagarkatti
Obesity is characterized by chronic low-grade, systemic inflammation, altered gut microbiota, and gut barrier disruption. Additionally, obesity is associated with increased activity of endocannabinoid system (eCB). However, the clear connection between gut microbiota and the eCB system in the regulation of energy homeostasis and adipose tissue inflammation and metabolism, remains to be established. We investigated the effect of treatment of mice with a cannabinoid receptor 1 (CB1) antagonist on Diet-Induced Obesity (DIO), specifically whether such a treatment that blocks endocannabinoid activity can induce changes in gut microbiota and anti-inflammatory state in adipose tissue...
November 15, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29115189/neuropsychological-feedback-services-improve-quality-of-life-and-social-adjustment
#6
Dana L Rosado, Susan Buehler, Emilie Botbol-Berman, Maia Feigon, Adeline León, Hien Luu, Carmen Carrión, Mitzi Gonzalez, Julia Rao, Taylor Grief, Michael Seidenberg, Neil H Pliskin
OBJECTIVE: In more recent years, studies have begun to examine levels of satisfaction of individuals or family members of individuals who undergo neuropsychological evaluation. However, to date there have been only a handful of formal studies that have specifically examined the role and contribution of neuropsychological assessment in patient care and management. This study sought to examine one specific component of neuropsychological assessment, namely the impact of patient feedback regarding neuropsychological testing on patient outcome...
November 8, 2017: Clinical Neuropsychologist
https://www.readbyqxmd.com/read/29070704/bivalent-complexes-of-prc1-with-orthologs-of-brd4-and-moz-morf-target-developmental-genes-in-drosophila
#7
Hyuckjoon Kang, Youngsook L Jung, Kyle A McElroy, Barry M Zee, Heather A Wallace, Jessica L Woolnough, Peter J Park, Mitzi I Kuroda
Regulatory decisions in Drosophila require Polycomb group (PcG) proteins to maintain the silent state and Trithorax group (TrxG) proteins to oppose silencing. Since PcG and TrxG are ubiquitous and lack apparent sequence specificity, a long-standing model is that targeting occurs via protein interactions; for instance, between repressors and PcG proteins. Instead, we found that Pc-repressive complex 1 (PRC1) purifies with coactivators Fs(1)h [female sterile (1) homeotic] and Enok/Br140 during embryogenesis. Fs(1)h is a TrxG member and the ortholog of BRD4, a bromodomain protein that binds to acetylated histones and is a key transcriptional coactivator in mammals...
October 1, 2017: Genes & Development
https://www.readbyqxmd.com/read/29056709/detection-of-leptospira-spp-in-water-turtle-trachemys-scripta-living-in-ponds-of-urban-parks
#8
Daniela Dezzutto, Raffaella Barbero, Giuseppina Canale, Pier Luigi Acutis, Cristina Biolatti, Andrea Dogliero, Mauthe Degerfeld Mitzy, Paola Francone, Alberto Colzani, Stefania Bergagna, Maria Silvia Gennero
Urban parks are green areas of cities where families and children spend hours outside. Turtles often inhabit urban parks. However, even if the animals seem harmless, they may serve as both reservoirs or accidental hosts for different serotypes of Leptospira spp. Leptospira spp. is a waterborne zoonotic bacterium relevant for public health. Reptiles and amphibians may play a role in the epidemiology, transmission, and persistence of Leptospira spp. In the present study, we observed the presence of anti-leptospiral agglutinins in a group of freshwater turtles (Trachemys scripta) captured in three urban ponds of the metropolitan city of Turin, Italy...
October 10, 2017: Veterinary Sciences
https://www.readbyqxmd.com/read/29037928/melanoma-of-the-foot-is-associated-with-advanced-disease-and-poorer-survival
#9
Barbara E Adams, Peter D Peng, Mitzi L Williams
The purpose of the present study was to review the outcomes and assess the prognostic factors associated with foot melanoma. We hypothesized that primary melanoma of the foot would be more likely to present at an advanced stage and be associated with poorer outcomes. Both univariate and multivariate analyses were conducted to examine the relationships between patients' demographic, clinical, and pathologic characteristics and deaths within 5 years. Categorical data were summarized as frequencies and percentages and continuous variables as mean ± standard deviation...
October 13, 2017: Journal of Foot and Ankle Surgery: Official Publication of the American College of Foot and Ankle Surgeons
https://www.readbyqxmd.com/read/29036605/survey-of-laboratory-practices-for-diagnosis-of-fungal-infection-in-seven-asian-countries-an-asia-fungal-working-group-afwg-initiative
#10
Ariya Chindamporn, Arunaloke Chakrabarti, Ruoyu Li, Pei-Lun Sun, Ban-Hock Tan, Mitzi Chua, Retno Wahyuningsih, Atul Patel, Zhengyin Liu, Yee-Chun Chen, Methee Chayakulkeeree
An online survey of mycology laboratories in seven Asian countries was conducted to assess the status, competence, and services available. Country representatives from the Asia Fungal Working Group (AFWG) contacted as many laboratories performing mycology diagnosis as possible in their respective countries, requesting that the laboratory heads complete the online survey. In total, 241 laboratories responded, including 71 in China, 104 in India, 11 in Indonesia, 26 in the Philippines, four in Singapore, 18 in Taiwan, and seven in Thailand...
September 20, 2017: Medical Mycology: Official Publication of the International Society for Human and Animal Mycology
https://www.readbyqxmd.com/read/29032988/clinical-and-radiographic-outcomes-after-total-shoulder-arthroplasty-with-an-anatomic-press-fit-short-stem
#11
Benjamin W Szerlip, Brent J Morris, Mitzi S Laughlin, Christopher M Kilian, T Bradley Edwards
BACKGROUND: There is limited information assessing outcomes after total shoulder arthroplasty (TSA) with a press-fit short stem. The purpose of this study was to evaluate early clinical and radiographic outcomes and complications after TSA with an anatomic press-fit short stem. METHODS: We identified 118 TSAs completed with press-fit short stem and minimum 2-year follow-up; 85 of these patients had a grit-blasted (GB) short stem placed, whereas 33 patients had short stems with proximal porous coating (PPC)...
October 9, 2017: Journal of Shoulder and Elbow Surgery
https://www.readbyqxmd.com/read/29025731/macrophage-depletion-using-clodronate-liposomes-decreases-tumorigenesis-and-alters-gut-microbiota-in-the-aom-dss-mouse-model-of-colon-cancer
#12
Jackie E Bader, Reilly T Enos, Kandy T Velazquez, Meredith S Carson, Mitzi Nagarkatti, Prakash S Nagarkatti, Ioulia Chatzistamou, J Mark Davis, James A Carson, Cory Robinson, E Angela Murphy
We examined the role of macrophages in inflammation associated colorectal cancer (CRC). Given the emerging evidence on immune-microbiota interactions in CRC, we also sought to examine the interaction between macrophages and gut microbiota. To induce CRC, male C57BL/6 mice (n=32) received a single injection of azoxymethane (AOM) followed by three cycles of dextran sodium sulfate (DSS) supplemented water at weeks 1, 4, and 7, respectively. Prior to the final DSS cycle (week 7) and twice weekly until sacrifice, mice (n=16/group) received either 200μl i...
October 12, 2017: American Journal of Physiology. Gastrointestinal and Liver Physiology
https://www.readbyqxmd.com/read/29017969/vigorous-exercise-mobilizes-cd34-hematopoietic-stem-cells-to-peripheral-blood-via-the-%C3%AE-2-adrenergic-receptor
#13
Nadia H Agha, Forrest L Baker, Hawley E Kunz, Rachel Graff, Rod Azadan, Chad Dolan, Mitzi S Laughlin, Chitra Hosing, Melissa M Markofski, Richard A Bond, Catherine M Bollard, Richard J Simpson
Acute dynamic exercise mobilizes CD34+ hematopoietic stem cells (HSCs) to the bloodstream, potentially serving as an economical adjuvant to boost the collection of HSCs from stem cell transplant donors. The mechanisms responsible for HSC mobilization with exercise are unknown but are likely due to hemodynamic perturbations, endogenous granulocyte-colony stimulating factor (G-CSF), and/or β2-adrenergic receptor (β2-AR) signaling. We characterized the temporal response of HSC mobilization and plasma G-CSF following exercise, and determined the impact of in vivo β-AR blockade on the exercise-induced mobilization of HSCs...
October 7, 2017: Brain, Behavior, and Immunity
https://www.readbyqxmd.com/read/28976179/trio-act-of-boronolectin-with-antibiotic-metal-complexed-macromolecules-toward-broad-spectrum-antimicrobial-efficacy
#14
Peng Yang, Marpe Bam, Parasmani Pageni, Tianyu Zhu, Yung Pin Chen, Mitzi Nagarkatti, Alan W Decho, Chuanbing Tang
Bacterial infections, particularly by Gram-negative pathogens, have become a serious threat to global healthcare due to the diminishing effectiveness of existing antibiotics. We report a nontraditional therapy to combine three components in one macromolecular system, in which boronic acid adheres to peptidoglycan or lipopolysaccharide via boron-polyol based boronolectin chemistry, cationic metal polymer frameworks interact with negatively charged cell membranes, and β-lactam antibiotics are reinstated with enhanced vitality to attack bacteria via evading the detrimental enzyme-catalyzed hydrolysis...
November 10, 2017: ACS Infectious Diseases
https://www.readbyqxmd.com/read/28972634/students-perceptions-of-a-poem-to-evaluate-learning-a-qualitative-study
#15
Mitzi M Saunders, Sonya L Kowalski, Suzanne Weathers
BACKGROUND: How best to use poetry in nursing education remains uncertain. This study explored students' perceptions of incorporating a poem into a final examination to evaluate learning in an advanced physical assessment course. METHOD: Qualitative design and method were used to collect and analyze data retrieved from private interviews with seven graduate nursing students. RESULTS: The themes were Being There, Think More, and Feeling Rushed...
October 1, 2017: Journal of Nursing Education
https://www.readbyqxmd.com/read/28951128/nemo-peptide-inhibits-the-growth-of-pancreatic-ductal-adenocarcinoma-by-blocking-nf-%C3%AE%C2%BAb-activation
#16
Zhuonan Zhuang, Hao Li, Harold Lee, Mitzi Aguilar, Takashi Gocho, Huaiqiang Ju, Tomonori Iida, Jianhua Ling, Jie Fu, Min Wu, Yichen Sun, Yu Lu, Paul J Chiao
NF-κB essential modulator (NEMO) binds and regulates IκB kinase (IKK) and is required for NF-κB activation. The NEMO-binding domain peptide (NBDP) of IKK was found to inhibit NF-κB activation and promote apoptosis in cancer cells. Studies have shown that constitutive NF-κB activation, one of the signature molecular alterations in pancreatic ductal adenocarcinoma (PDAC), is a potential therapeutic target. However, preclinical and therapeutic evidence that supports direct targeting of IKK activation in therapy is lacking...
September 23, 2017: Cancer Letters
https://www.readbyqxmd.com/read/28867182/deficiency-of-klf4-compromises-the-lung-function-in-an-acute-mouse-model-of-allergic-asthma
#17
Jeanette A Nimpong, Wintana Gebregziabher, Udai P Singh, Prakash Nagarkatti, Mitzi Nagarkatti, Johnie Hodge, Chunming Liu, Daping Fan, Walden Ai
Asthma is a chronic inflammatory disease of the airways and the mechanisms are not fully understood. Myeloid-derived suppressor cells (MDSCs) are a heterogeneous group of monocytes, granulocyte and myeloid cells at early stage of differentiation. They possess phenotypic plasticity and regulate airway inflammation. We recently reported that Kruppel-like factor 4 (KLF4) regulates MDSC differentiation into fibrocytes, emerging effectors in chronic inflammation. However, the role of KLF4 in asthma is not known...
November 4, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28857632/adherence-persistence-and-discontinuation-among-hispanic-and-african-american-patients-with-multiple-sclerosis-treated-with-fingolimod-or-glatiramer-acetate
#18
Mitzi J Williams, Kristen Johnson, Helen M Trenz, Stephanie Korrer, Rachel Halpern, Yujin Park, Vivian Herrera
OBJECTIVE: Few studies have examined compliance to disease-modifying therapies (DMTs) for multiple sclerosis (MS) in minority populations. This study compared adherence, discontinuation, and persistence for fingolimod (FTY) and glatiramer acetate (GA) initiators among Hispanic and African American patients with MS. METHODS: This retrospective claims data study examined Hispanic and African American adults with MS who initiated FTY or GA between September 1, 2010 and June 30, 2014...
October 3, 2017: Current Medical Research and Opinion
https://www.readbyqxmd.com/read/28857382/nurse-manager-perspective-of-staff-participation-in-unit-level-shared-governance
#19
Sheila Cox Sullivan, Mitzi R Norris, Lana M Brown, Karen J Scott
AIM: To examine the nurse manager perspective surrounding implementation of unit level shared governance in one Veterans Health Administration facility. BACKGROUND: Nursing shared governance is a formal model allowing nursing staff decision-making input into clinical practice, quality improvement, evidence-based practice and staff professional development. Unit level shared governance is a management process where decision authority is delegated to nursing staff at the unit level...
August 30, 2017: Journal of Nursing Management
https://www.readbyqxmd.com/read/28851787/a-qualitative-study-exploring-the-social-and-environmental-context-of-recently-acquired-hiv-infection-among-men-who-have-sex-with-men-in-south-east-england
#20
Annabelle Gourlay, Julie Fox, Mitzy Gafos, Sarah Fidler, Nneka Nwokolo, Amanda Clarke, Richard Gilson, Chloe Orkin, Simon Collins, Kholoud Porter, Graham Hart
OBJECTIVES: A key UK public health priority is to reduce HIV incidence among gay and other men who have sex with men (MSM). This study aimed to explore the social and environmental context in which new HIV infections occurred among MSM in London and Brighton in 2015. DESIGN: A qualitative descriptive study, comprising in-depth interviews, was carried out as a substudy to the UK Register of HIV Seroconverters cohort: an observational cohort of individuals whose date of HIV seroconversion was well estimated...
August 28, 2017: BMJ Open
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