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myocardiac apoptosis

Mei-Chi Chang, Yi-Jane Chen, Eric Jein-Wein Liou, Wan-Yu Tseng, Chiu-Po Chan, Hseuh-Jen Lin, Wan-Chuen Liao, Ya-Ching Chang, Po-Yuan Jeng, Jiiang-Huei Jeng
Cardiovascular diseases (atherosclerosis, stroke, myocardiac infarction etc.) are the major systemic diseases of elder peoples in the world. This is possibly due to increased levels of oxidized low-density lipoproteins (oxLDLs) such as 7-ketocholesterol (7-KC) and lysophosphatidylcholine (LPC) that damage vascular endothelial cells, induce inflammatory responses, to elevate the risk of cardiovascular diseases, Alzheimer's disease, and age-related macular degeneration. However the toxic effects of 7-KC on endothelial cells are not known...
November 15, 2016: Oncotarget
C Li, C Zhang, T Wang, J Xuan, C Su, Y Wang
BACKGROUND: Although recent studies have found that heme oxygenase (HO)-1 plays an important role in myocardiac cell survival, the precise mechanisms occurring during hypoxia/reoxygenation (H/R) injury remain unclear. Therefore, the aim of this study was to investigate the cytoprotective mechanisms of HO-1 against H/R-induced myocardiac cell apoptosis and to explore whether the Akt signaling pathway contributed to the protection provided by HO-1. METHODS: Cobalt protoporphyrin (CoPP, a pharmacologic inducer of HO-1) was employed to induce the over-expression of HO-1 before H/R in H9c2 cells...
December 2016: Herz
Lu Ye, Shuangqiang Yang
OBJECTIVE: To investigate the effects of Sirtuin 3 on cytochrome C (CytC) release and caspase-3 activation in myocardiac H9c2 cells with calcium overload. METHODS: H9c2 cells were randomly divided into four groups: control group, Sirtuin 3 group, ionomycin group, ionomycin & Sirtuin 3 group. Myocyte calcium overload models were established by ionomycin revulsion. The levels of CytC and caspase-3 in mitochondria and cytoplasm were examined by Western blotting. Intracellular caspase-3 activity was examined by spectrophotometry...
August 2015: Xi Bao Yu Fen Zi Mian Yi Xue za Zhi, Chinese Journal of Cellular and Molecular Immunology
Zhong-wei Liu, Jun-kui Wang, Chuan Qiu, Gong-chang Guan, Xin-hong Liu, Shang-jian Li, Zheng-rong Deng
AIM: Matrine is an alkaloid from Sophora alopecuroides L, which has shown a variety of pharmacological activities and potential therapeutic value in cardiovascular diseases. In this study we examined the protective effects of matrine against diabetic cardiomyopathy (DCM) in rats. METHODS: Male SD rats were injected with streptozotocin (STZ) to induce DCM. One group of DCM rats was pretreated with matrine (200 mg·kg(-1)·d(-1), po) for 10 consecutive days before STZ injection...
March 2015: Acta Pharmacologica Sinica
Meng Jiang, Lin Wang, Hai-He Jiang
OBJECTIVE: To explore the role of spinal MAPK-ERK signal pathway in myocardial ischemia-reperfusion (I/R) injury. METHODS: Sixty male Sprague-Dawley(SD) rats (80-100 g) were randomly divided into 3 groups: sham (n=10), PD98059 (n=25) and I/R groups (n=25). Three days after successful intrathecal implantation, 5 μg DMSO was injected intrathecally into the sham group, and then the left coronary arteries were separated without being tied. Rats in the I/R and PD98059 groups were injected with 5 μL DMSO and PD98059 (5 μg) 30 minutes before thoractomy respectively...
May 2013: Zhongguo Dang Dai Er Ke za Zhi, Chinese Journal of Contemporary Pediatrics
Fahir Baraković, Zumreta Kusljugić, Izet Masić
Diagnosis of primarly cardiomypathies refers to genes discorders in chromosomes. Aim of this paper is to show genetics and molecular knowledges published so far. Familiar form hypertrophic cardiomyopathy is hereditable autosomatically dominantly in any of 10 genes that regulate contractile, structural and regulative function with predomination of mutation in gene for heavy chaire of myocardiac beta myosin localized at 14 chromosome (more than 200 mutation). Sporadic forms appears autosomatically recessively as the result of new mutation or as non-genetic form...
2007: Medicinski Arhiv
Xiao-ping LI, Lang LI, Rui-zhen CHEN, Tang-wei LIU, Wei-feng WU, E SHEN, Ying-zhen YANG, Hao-zhu CHEN
OBJECTIVE: in septic mice, myocardial calpain was activated and induced caspase-3 activation, the association between calpain activation and apoptosis was explored in this experiment. METHODS: in in vivo model, adult C57 mice were injected with lipopolysaccharide (LPS, 4 mg/kg, i.p.) to induce sepsis. Myocardial calpain and caspase-3 activities, protein levels of calpain-1, calpain-2, calpastatin, Bcl-2 and Bid were detected by Western blot analysis and myocardial apoptosis was detected by TUNEL, myocardiac function was evaluated by Langendorff system...
September 2010: Zhonghua Xin Xue Guan Bing za Zhi
Mu-Jang Lee, Han-Min Chen, Bor-Show Tzang, Chiu-Wen Lin, Chau-Jong Wang, Jer-Yuh Liu, Shao-Hsuan Kao
Increased cell death of cardiomyocyte by oxidative stress is known to cause dysfunction of the heart. O. gratissimum is one of the more well-known medicinal plants among the Ocimum species and widely used in treatment of inflammatory diseases. In this study, we hypothesized that aqueous extract of O. gratissimum leaf (OGE) may protect myocardiac cell H9c2 from oxidative injury by hydrogen peroxide (H(2)O(2)). Our results revealed that OGE pretreatment dose-dependently protects H9c2 cells from cell death when exposed to H(2)O(2)...
2011: Evidence-based Complementary and Alternative Medicine: ECAM
Linda Chularojmontri, Yoshito Ihara, Eiji Muroi, Shinji Goto, Takahito Kondo, Suvara K Wattanapitayakul
OBJECTIVE: To examine cytoprotective effect of Phyllanthus urinaria (PU) ethanolic extract in doxorubicin (DOX)-induced toxicity. The research focus was on the mechanism of action in association with the expression and localization of glutathione-S transferase (GST) in cardiac H9c2 cells. MATERIAL AND METHOD: The presence of GST isoforms was evaluated in H9c2 cells using western blot analysis and confocal immunofluorescence visualization. Cells were then treated with DOX in the presence and absence of PU and several cytoprotective indices were evaluated, including the expression of the rate-limiting enzyme for glutathione synthesis, gamma-glutamylcysteine synthetase (gamma-GCS), manganese superoxide dismutase (MnSOD), copper-zinc SOD (CuZnSOD), and GST activity from cell lysate...
June 2009: Journal of the Medical Association of Thailand, Chotmaihet Thangphaet
Xiao-Qiao Wu, Yong-Hong Li, Xian Zhu, Mao-Wang Wu, Sheng-Ming Hu
OBJECTIVE: To study the pathogenesis of viral myocarditis (VMC) and dilated cardiomyopathy (DCM) and their relationship. METHODS: Sixty samples including 20 VMC, 20 DCM and 20 controls were collected. The expression of Fas protein in myocardium of each group was detected by modified immunohistochemistry with unequivocal brown staining in the myocardial membrane scored as positive, and the results of positive reaction were analyzed by Ridit test. RESULTS: Fas protein expression increased obviously in VMC and DCM groups as compared with that of the control group...
December 2008: Fa Yi Xue za Zhi
Fernando Soler, Antonio Lax, Francisco Fernández-Belda
Experimental sarcoplasmic reticulum damage induced by 3 microM thapsigargin or 1 microg/ml tunicamycin provoked viability loss of the cell population in approximately 72 h. Release of cytochrome c from mitochondria was an early event and Bax translocation to the mitochondria preceded or was simultaneous with cytochrome c release. The release of cytochrome c was not related with mitochondria depolarization or caspase activation. Irreversible stress in the sarcoplasmic reticulum, detected by the early activation of caspase 12, was functionally linked to the mitochondrial apoptotic pathway...
October 15, 2007: Archives of Biochemistry and Biophysics
Gábor Jancsó, Barbara Cserepes, Balázs Gasz, László Benkó, Balázs Borsiczky, Andrea Ferenc, Mária Kürthy, Boglárka Rácz, János Lantos, János Gál, Endre Arató, Lószló Sínayc, György Wéber, Erzsébet Róth
In the study the authors aimed to demonstrate the expression and protective effect of heme oxygenase-1 (HO-1) in the delayed preconditioning (PC) on cultured myocardiac cells. Neonatal rat cardiac myocytes were exposed to ischemic (ischemic medium [IM] for 20 min) and pharmacological (adenosine, epinephrine, opioid) PC. Twenty-four hours later cells were subjected to a simulated ischemia (SI)--culturing for 3 h in IM, followed by 2-h reperfusion in normal medium--and then lactate dehydrogenase (LDH), live/death ratio, and apoptosis were measured...
January 2007: Annals of the New York Academy of Sciences
Antonio Lax, Fernando Soler, Francisco Fernández-Belda
The incubation of H9c2 cells with 10 microM thapsigargin (TG) was associated with the appearance of a two-component cytoplasmic Ca2+ peak. Experiments performed in a Ca2+-free medium indicated that both components came from intracellular sources. The first component of the signal corresponded to the discharge of the sarco-endoplasmic reticulum (SER) Ca2+ store. The appearance of the second component was prevented by cell preincubation with cyclosporin A (CsA) and gave rise to a clear and permanent depolarization of the mitochondrial inner membrane...
September 2006: Biochimica et Biophysica Acta
Yoshishige Urata, Yoshito Ihara, Hiroaki Murata, Shinji Goto, Takehiko Koji, Junji Yodoi, Satoshi Inoue, Takahito Kondo
The GSH/glutaredoxin (GRX) system is involved in the redox regulation of certain enzyme activities, and this system protects cells from H2O2-induced apoptosis by regulating the redox state of Akt (Murata, H., Ihara, Y., Nakamura, H., Yodoi, J., Sumikawa, K., and Kondo, T. (2003) J. Biol. Chem. 278, 50226-50233). Estrogens, such as 17beta-estradiol (E2), play an important role in development, growth, and differentiation and appear to have protective effects on oxidative stress mediated by estrogen receptor alpha (ERalpha)...
May 12, 2006: Journal of Biological Chemistry
Yoshito Ihara, Yoshishige Urata, Shinji Goto, Takahito Kondo
Calreticulin (CRT), a Ca2+-binding molecular chaperone in the endoplasmic reticulum, plays a vital role in cardiac physiology and pathology. Oxidative stress is a main cause of myocardiac apoptosis in the ischemic heart, but the function of CRT under oxidative stress is not fully understood. In the present study, the effect of overexpression of CRT on susceptibility to apoptosis under oxidative stress was examined using myocardiac H9c2 cells transfected with the CRT gene. Under oxidative stress due to H2O2, the CRT-overexpressing cells were highly susceptible to apoptosis compared with controls...
January 2006: American Journal of Physiology. Cell Physiology
Chie Yasuoka, Yoshito Ihara, Satoshi Ikeda, Yoshiyuki Miyahara, Takahito Kondo, Shigeru Kohno
Cell survival signaling of the Akt/protein kinase B pathway was influenced by a change in the cytoplasmic free calcium concentration ([Ca2+]i) for over 2 h via the regulation of a Ser/Thr phosphatase, protein phosphatase 2Ac (PP2Ac), in rat myocardiac H9c2 cells. Akt was down-regulated when [Ca2+]i was elevated by thapsigargin, an inhibitor of the endoplasmic reticulum Ca(2+)-ATPase, but was up-regulated when it was suppressed by 1,2-bis(o-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid tetra(acetoxymethyl)ester (BAPTA-AM), a cell permeable Ca2+ chelator...
December 3, 2004: Journal of Biological Chemistry
Yong-jun Li, Wen-hui Ding, Wei Gao, Yong Huo, Tao Hong, Ren-ying Zhu, Da-long Ma
OBJECTIVE: To observe the dynamic changes of plasma inflammatory cytokine interleukin-1 beta (IL-1 beta) in patients with acute myocardiac infarction (AMI) before and after recanalization of the infarct related artery (IRA) and to observe the effect of recombinant human IL-1 receptor antagonist (rhIL-1ra) on the postischemic reperfused myocardium in experimental rabbits. METHODS: (1) ELISA was used to measure the plasma IL-1 beta of 22 AMI patients, 20 males and 2 females, aged 64 +/- 12, before emergency percutaneous coronary intervention (PCI), and 12 hours and 24 hours after-intervention, and measure the plasma IL-1 beta of 8 healthy controls, 6 males and 2 females, aged 56 +/- 9...
April 2, 2004: Zhonghua Yi Xue za Zhi [Chinese medical journal]
Liang-qing Zhang, Jun-fa Xu, Kang-rong Cai, Li-zhi Li
OBJECTIVE: To investigate the effect of adenosine preconditioning on cardiac myocyte apoptosis and the expression of nuclear factor-kappaB (NF-kappaB)during myocardial ischemia/reperfusion (I/R) in rats. METHODS: A model of I/R injury (I/R group) and pretreatment with adenosine (AP group) was reproduced in rats. The morphologic features of apoptosis were identified histochemically by TdT-mediated dUTP nick end (TUNEL) staining. Expression of NF-kappaB in apoptosis myocardiac cells was observed by immunohistochemical stain and image analysis...
March 2004: Zhongguo Wei Zhong Bing Ji Jiu Yi Xue, Chinese Critical Care Medicine, Zhongguo Weizhongbing Jijiuyixue
D S Choi, S J Ward, N Messaddeq, J M Launay, L Maroteaux
During embryogenesis, serotonin has been reported to be involved in craniofacial and cardiovascular morphogenesis. The detailed molecular mechanisms underlying these functions, however remain unknown. From mouse and human species, we have recently reported the cloning of 5-HT2B receptors which share signal transduction pathways with other 5-HT2 receptor subtypes (5-HT2A and 5-HT2C). In addition to phospholipase C stimulation, it appears that these three subtypes of receptor transduce a common serotonin-induced mitogenic activity, which could be important for cell differentiation and proliferation...
May 1997: Development
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