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https://www.readbyqxmd.com/read/28527179/making-time-conservation-of-biological-clocks-from-fungi-to-animals
#1
Jay C Dunlap, Jennifer J Loros
The capacity for biological timekeeping arose at least three times through evolution, in prokaryotic cyanobacteria, in cells that evolved into higher plants, and within the group of organisms that eventually became the fungi and the animals. Neurospora is a tractable model system for understanding the molecular bases of circadian rhythms in the last of these groups, and is perhaps the most intensively studied circadian cell type. Rhythmic processes described in fungi include growth rate, stress responses, developmental capacity, and sporulation, as well as much of metabolism; fungi use clocks to anticipate daily environmental changes...
May 2017: Microbiology Spectrum
https://www.readbyqxmd.com/read/28521843/high-prevalence-of-trichomonas-gallinae-in-wild-columbids-across-western-and-southern-europe
#2
Melanie Marx, Gerald Reiner, Hermann Willems, Gregorio Rocha, Klaus Hillerich, Juan F Masello, Sylvia L Mayr, Sarah Moussa, Jenny C Dunn, Rebecca C Thomas, Simon J Goodman, Keith C Hamer, Benjamin Metzger, Jacopo G Cecere, Fernando Spina, Steffen Koschkar, Luciano Calderón, Tanja Romeike, Petra Quillfeldt
BACKGROUND: Avian trichomonosis is known as a widespread disease in columbids and passerines, and recent findings have highlighted the pathogenic character of some lineages found in wild birds. Trichomonosis can affect wild bird populations including endangered species, as has been shown for Mauritian pink pigeons Nesoenas mayeri in Mauritius and suggested for European turtle doves Streptopelia turtur in the UK. However, the disease trichomonosis is caused only by pathogenic lineages of the parasite Trichomonas gallinae...
May 18, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/28516859/recombinant-esterase-from-corynebacterium-pseudotuberculosis-in-dna-and-subunit-recombinant-vaccines-partially-protects-mice-against-challenge
#3
Alexandre Antunes Brum, Andrea de Fatima Silva Rezende, Francisco Silvestre Brilhante, Thais Collares, Karine Begnine, Fabiana Kommling Seixas, Tiago Veiras Collares, Odir Antônio Dellagostin, Vasco Azevedo, Anderson Santos, Ricardo Wagner Portela, Sibele Borsuk
PURPOSE: We tested the efficacy of the esterase encoded by cp1002_RS09720 from Corynebacteriumpseudotuberculosis in recombinant subunit and DNA caseous lymphadenitis (CLA) vaccines. This target was predicted as one of the best CLA vaccine candidates by mature epitope density analysis. METHODOLOGY: Gene cp1002_RS09720 was cloned into two different vectors (pAE for subunit vaccine and pTARGET for DNA vaccine). Four groups of 15 mice each were immunized with the recombinant esterase rCP09720 associated with aluminium hydroxide adjuvant (G1), pTARGET/cp09720 DNA vaccine (G2), a naked pTARGET (G3) or PBS as a negative control (G4)...
May 2017: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28513491/results-of-a-new-mirror-tuck-technique-for-fixation-of-lacrimal-bypass-tube-in-conjunctivodacryocystorhinostomy
#4
Ruchi Goel, Divya Kishore, Smriti Nagpal, Sushil Kumar, Neha Rathie
CONTEXT: Conjunctivodacryocystorhinostomy (CDCR) is the procedure of choice for proximal canalicular blocks. However, the complications of tube migration and extrusion limit its widespread practice. AIM: The aim of this study is to evaluate the efficacy and complications of the new "mirror tuck technique" for fixation of lacrimal bypass glass tube without holes in proximal canalicular blocks in laser CDCR. MATERIALS AND METHODS: A prospective interventional study was conducted in forty consecutive eyes of adult patients, undergoing 980 nm diode laser CDCR for proximal canalicular blocks...
April 2017: Indian Journal of Ophthalmology
https://www.readbyqxmd.com/read/28504618/butyrate-reduces-high-fat-diet-induced-metabolic-alterations-hepatic-steatosis-and-pancreatic-beta-cell-and-intestinal-barrier-dysfunctions-in-prediabetic-mice
#5
V A Matheus, Lcs Monteiro, R B Oliveira, D A Maschio, C B Collares-Buzato
In this study, we investigated the effect of diet supplementation with sodium butyrate (5% w/w), a short-chain fatty acid produced by the intestinal microbiota, on metabolic parameters, body adiposity, hepatic and pancreatic lipid accumulation, beta cell function/mass as well as on the structure and function of the tight junction-mediated intestinal epithelial barrier in both normal and obese/prediabetic C57 mice fed a regular (control) or high-fat diet for 60 days, respectively. Butyrate treatment significantly inhibited all the high-fat-induced metabolic dysfunctions evaluated, i...
January 1, 2017: Experimental Biology and Medicine
https://www.readbyqxmd.com/read/28502899/the-expression-of-p450-genes-mediating-fenpropathrin-resistance-is-regulated-by-cncc-and-maf-in-tetranychus-cinnabarinus-boisduval
#6
Li Shi, Mengyao Wang, Yichao Zhang, Guangmao Shen, Haishan Di, Yue Wang, Lin He
Although overexpression of genes encoding detoxification enzymes is a well-known mechanism of pesticide resistance of mites, the regulators involved in this process are still illiterate. Previous studies in our laboratory demonstrated that the overexpression of six P450 genes contributes to fenpropathrin resistance in T. cinnabarinus. In this study, six transcription factor genes that likely regulate the expression of P450 genes were identified and characterized. Quantitative PCR (qPCR) analysis showed that three transcription factor genes were highly expressed in a fenpropathrin-resistant (FeR) strain of T...
May 11, 2017: Comparative Biochemistry and Physiology. Toxicology & Pharmacology: CBP
https://www.readbyqxmd.com/read/28501121/validation-of-a-field-spinal-motion-restriction-protocol-in-a-level-i-trauma-center
#7
James M Tatum, Nicolas Melo, Ara Ko, Navpreet K Dhillon, Eric J T Smith, Dorothy A Yim, Galinos Barmparas, Eric J Ley
BACKGROUND: Spinal motion restriction (SMR) after traumatic injury has been a mainstay of prehospital trauma care for more than 3 decades. Recent guidelines recommend a selective approach with cervical spine clearance in the field when criteria are met. MATERIALS AND METHODS: In January 2014, the Department of Health Services of the City of Los Angeles, California, implemented revised guidelines for cervical SMR after blunt mechanism trauma. Adult patients (aged ≥18 y) with an initial Glasgow Coma Scale (GCS) score of ≥13 presented to a single level I trauma center after blunt mechanism trauma over the following 1-y period were retrospectively reviewed...
May 1, 2017: Journal of Surgical Research
https://www.readbyqxmd.com/read/28488626/surgery-for-superior-hypophyseal-artery-aneurysms-a-new-classification-and-surgical-considerations
#8
Chaitanya Godbole, Sanjay Behari, Kamlesh K Bhaisora, Jayesh Sardhara, Arun Srivastava, Anant Mehrotra, Kuntal Kanti Das, Raj Kumar, Awadhesh K Jaiswal
BACKGROUND: Superior hypophyseal artery (SHA) aneurysms form a unique subgroup of paraclinoid aneurysms having a propensity to grow to a large size in the suprasellar region resulting in compression of the optic nerve, chiasma, and/or tract. AIM: A new classification of SHA aneurysms is proposed that helps in identifying the surgical issues encountered during surgical clipping of these medially directed aneurysms located at different segments of the medial surface of the internal carotid artery (ICA)...
May 2017: Neurology India
https://www.readbyqxmd.com/read/28486433/human-indoor-exposure-to-airborne-halogenated-flame-retardants-influence-of-airborne-particle-size
#9
Mark J La Guardia, Erika D Schreder, Nancy Uding, Robert C Hale
Inhalation of halogenated flame-retardants (HFRs) released from consumer products is an important route of exposure. However, not all airborne HFRs are respirable, and thus interact with vascular membranes within the gas exchange (alveolar) region of the lung. HFRs associated with large (>4 µm), inhalable airborne particulates are trapped on the mucosal lining of the respiratory tract and then are expelled or swallowed. The latter may contribute to internal exposure via desorption from particles in the digestive tract...
May 9, 2017: International Journal of Environmental Research and Public Health
https://www.readbyqxmd.com/read/28464079/effects-of-roughage-inclusion-and-particle-size-on-digestion-and-ruminal-fermentation-characteristics-of-beef-steers
#10
C P Weiss, W W Gentry, C M Meredith, B E Meyer, N A Cole, L O Tedeschi, F T McCollum, J S Jennings
Roughage is fed in finishing diets to promote ruminal health and decrease digestive upset, but the inclusion rate is limited because of the cost per unit of energy and feed management issues. Rumination behavior of cattle may be a means to standardize roughage in beef cattle finishing diets, and increasing the particle size of roughage could modulate the ruminal environment and aid in maintaining ruminal pH. Therefore, this experiment was conducted to determine the effects of corn stalk (CS) inclusion rate and particle size in finishing diets on digestibility, rumination, and ruminal fermentation characteristics of beef steers...
April 2017: Journal of Animal Science
https://www.readbyqxmd.com/read/28445809/inhibition-of-14q32-microrna-mir-495-reduces-lesion-formation-intimal-hyperplasia-and-plasma-cholesterol-levels-in-experimental-restenosis
#11
Sabine M J Welten, Rob C M de Jong, Anouk Wezel, Margreet R de Vries, Martin C Boonstra, Laura Parma, J Wouter Jukema, Tetje C van der Sluis, Ramon Arens, Ilze Bot, Sudhir Agrawal, Paul H A Quax, A Yaël Nossent
BACKGROUND AND AIMS: We aimed at investigating the role of 14q32 microRNAs in intimal hyperplasia and accelerated atherosclerosis; two major contributors to restenosis. Restenosis occurs regularly in patients treated for coronary artery disease and peripheral arterial disease. We have previously shown that inhibition of 14q32 microRNAs leads to increased post-ischemic neovascularization, and microRNA miR-494 also decreased atherosclerosis, while increasing plaque stability. We hypothesized that 14q32 microRNA inhibition has beneficial effects on intimal hyperplasia, as well as accelerated atherosclerosis...
April 13, 2017: Atherosclerosis
https://www.readbyqxmd.com/read/28429117/a-comparison-of-mcgrath-mac%C3%A2-and-standard-direct-laryngoscopy-in-simulated-immobilized-cervical-spine-pediatric-intubation-a-manikin-study
#12
Marcin Madziala, Jacek Smereka, Marek Dabrowski, Steve Leung, Kurt Ruetzler, Lukasz Szarpak
Emergency airway management in children is generally considered to be challenging, and endotracheal intubation requires a high level of personal skills and experience. Immobilization of the cervical spine is indicated in all patients with the risk of any cervical spine injury but significantly aggravates endotracheal intubation. The best airway device in this setting has not been established yet, although the use of videolaryngoscopes is generally promising. Seventy-five moderately experienced paramedics of the Emergency Medical Service of Poland performed endotracheal intubations in a pediatric manikin in three airway scenarios: (A) normal airway, (B) manual in-line cervical immobilization, and (C) cervical immobilization using a Patriot cervical extrication collar and using two airway techniques: (1) McGrath videolaryngoscope and (2) Macintosh blade in a randomized sequence...
April 21, 2017: European Journal of Pediatrics
https://www.readbyqxmd.com/read/28420542/improving-prehospital-trauma-care-in-rwanda-through-continuous-quality-improvement-an-interrupted-time-series-analysis
#13
John W Scott, Jeanne D'Arc Nyinawankusi, Samuel Enumah, Rebecca Maine, Eric Uwitonze, Yihan Hu, Ignace Kabagema, Jean Claude Byiringiro, Robert Riviello, Sudha Jayaraman
INTRODUCTION: Injury is a major cause of premature death and disability in East Africa, and high-quality pre-hospital care is essential for optimal trauma outcomes. The Rwandan pre-hospital emergency care service (SAMU) uses an electronic database to evaluate and optimize pre-hospital care through a continuous quality improvement programme (CQIP), beginning March 2014. MATERIALS AND METHODS: The SAMU database was used to assess pre-hospital quality metrics including supplementary oxygen for hypoxia (O2), intravenous fluids for hypotension (IVF), cervical collar placement for head injuries (c-collar), and either splinting (splint) or administration of pain medications (pain) for long bone fractures...
April 5, 2017: Injury
https://www.readbyqxmd.com/read/28417524/biofilms-of-candida-spp-from-the-ocular-conjunctiva-of-horses-with-reduced-azole-susceptibility-a-complicating-factor-for-the-treatment-of-keratomycosis
#14
Raimunda Sâmia Nogueira Brilhante, Paula Vago Bittencourt, Débora de Souza Collares Castelo-Branco, Glaucia Morgana de Melo Guedes, Jonathas Sales de Oliveira, Lucas Pereira Alencar, Rossana de Aguiar Cordeiro, Mariana Pinheiro, Evilázio Fernandes Nogueira-Filho, Waldemiro de Aquino Pereira-Neto, José Júlio Costa Sidrim, Marcos Fábio Gadelha Rocha
OBJECTIVES: This study aimed to assess the biofilm-forming ability of Candida spp. from the ocular conjunctiva of horses and to investigate the antifungal susceptibility of these biofilms. PROCEDURES: Initially, the biofilm-forming ability of 15 strains was assessed by crystal violet staining, which reveals the fungal biomass adhered to the polystyrene plates, and scanning electron microscopy. Then, the minimum inhibitory concentrations (MICs) of amphotericin B, fluconazole, itraconazole, and caspofungin were initially determined against strains in planktonic form...
April 18, 2017: Veterinary Ophthalmology
https://www.readbyqxmd.com/read/28408149/leptinotarsa-cap-n-collar-isoform-c-kelch-like-ech-associated-protein%C3%A2-1-signaling-is-critical-for-the-regulation-of-ecdysteroidogenesis-in-the-larvae
#15
Qiang-Kun Sun, Qing-Wei Meng, Qing-Yu Xu, Pan Deng, Wen-Chao Guo, Guo-Qing Li
Drosophila cap 'n' collar isoform C (CncC) and Kelch-like ECH associated protein 1 (Keap1) regulate metamorphosis by transcriptional control of a subset of genes involved in ecdysteroidogenesis, 20-hydroxyecdysone (20E) signaling, and juvenile hormone (JH) degradation. In the present paper, we found that prothoracicotropic hormone signal was required for the activation of LdCncC and LdKeap1 in Leptinotarsa decemlineata. Moreover, RNA interference of LdCncC or LdKeap1 in the fourth-instar larvae delayed development...
April 10, 2017: Insect Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/28395497/-assessment-and-surgical-treatment-for-58-substernal-goiter
#16
X Zhu, Z C Huang, X Feng, F Tao
Objective: To investigate the diagnosis and treatment of substernal goiter. Methods: A total of 58 cases with substernal goiters undergoing surgery between January 2005 and December 2015 were analysed retrospectively. There were 10 males and 48 females and their age ranged from 38 to 82 years with a median age of 58 years. According to enhanced CT and clinical signs of substernal goiters, there were 40 cases for typeⅠ, 17 cases for type Ⅱ, and 1 case for type Ⅲ. Results: Patients with typeⅠand Ⅱ substernal goiters (8 cases of papillary carcinoma) underwent low neck and collar-type incision, and patients with type Ⅲ substernal goiters received carotid chest surgery...
March 7, 2017: Zhonghua Er Bi Yan Hou Tou Jing Wai Ke za Zhi, Chinese Journal of Otorhinolaryngology Head and Neck Surgery
https://www.readbyqxmd.com/read/28393557/recurrent-stingers-in-an-adolescent-football-player-dilemmas-of-return-to-play-a-case-report-and-review-of-the-literature
#17
Jason L Zaremski, MaryBeth Horodyski, Daniel C Herman
We present the case of a 16-year-old football linebacker with a history of recurrent stingers. Initial physical examination was normal as were cervical spine radiographs. MRI of the cervical spine revealed relative stenosis. Electrodiagnostic testing revealed chronic bilateral neurogenic changes of the superior trunk of the brachial plexus. A Kerr Collar was obtained to minimize head acceleration and force transmission through the neck. While there are return-to-play guidelines for recurrent stingers, there are inconsistencies with those recommendations...
April 10, 2017: Research in Sports Medicine
https://www.readbyqxmd.com/read/28382855/natural-infections-with-pigeon-paramyxovirus-serotype-1-pathologic-changes-in-eurasian-collared-doves-streptopelia-decaocto-and-rock-pigeons-columba-livia-in-the-united-states
#18
M Isidoro-Ayza, C L Afonso, J B Stanton, S Knowles, H S Ip, C L White, H Fenton, M G Ruder, A C Dolinski, J Lankton
Pigeon paramyxovirus serotype 1 (PPMV-1) is a globally distributed, virulent member of the avian paramyxovirus serotype 1 serogroup that causes mortality in columbiformes and poultry. Following introduction into the United States in the mid-1980s, PPMV-1 rapidly spread causing numerous mortality events in Eurasian collared-doves ( Streptopelia decaocto) (ECDOs) and rock pigeons ( Columba livia) (ROPIs). The investigators reviewed pathological findings of 70 naturally infected, free-ranging columbiforms from 25 different mortality events in the United States...
January 1, 2017: Veterinary Pathology
https://www.readbyqxmd.com/read/28373929/work-related-correlates-of-occupational-sitting-in-a-diverse-sample-of-employees-in-midwest-metropolitan-cities
#19
Lin Yang, J Aaron Hipp, Jung Ae Lee, Rachel G Tabak, Elizabeth A Dodson, Christine M Marx, Ross C Brownson
The worksite serves as an ideal setting to reduce sedentary time. Yet little research has focused on occupational sitting, and few have considered factors beyond the personal or socio-demographic level. The current study i) examined variation in occupational sitting across different occupations, ii) explored whether worksite level factors (e.g., employer size, worksite supports and policies) may be associated with occupational sitting. Between 2012 and 2013, participants residing in four Missouri metropolitan areas were interviewed via telephone and provided information on socio-demographic characteristics, schedule flexibility, occupation, work related factors, and worksite supports and policies...
June 2017: Preventive Medicine Reports
https://www.readbyqxmd.com/read/28341929/molecular-characterisation-of-four-echinostomes-digenea-echinostomatidae-from-birds-in-new-zealand-with-descriptions-of-echinostoma-novaezealandense-n-sp-and-echinoparyphium-poulini-n-sp
#20
Simona Georgieva, Isabel Blasco-Costa, Aneta Kostadinova
Morphological and molecular characterisation of echinostome specimens (Digenea: Echinostomatidae) recovered in one Anas platyrhynchos L. and one Cygnus atratus (Latham) (Anseriformes: Anatidae) from New Zealand revealed the presence of two known species, Echinostoma miyagawai Ishii, 1932 and Echinoparyphium ellisi (Johnston & Simpson, 1944) and two species new to science. Comparative morphological and phylogenetic analyses supported the distinct species status of Echinostoma novaezealandense n. sp. ex Branta canadensis (L...
March 24, 2017: Systematic Parasitology
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