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Xiaobo Wang, Jielian Wu, Shaoqing Jian, Gang Yang, Baoqing Hu, Chungen Wen
The small Mafs, MafF, MafG and MafK play critical roles in morphogenesis and homeostasis through associating with Cap "n" Collar family of transcription factors. In this study, we tried to identify a small Maf protein in the freshwater mussel Cristaria plicata. The MafK cDNA of C. plicata, designated as CpMafK, was cloned from the hemocytes using degenerate primers by the rapid amplification of cDNA ends PCR. The full length cDNA of CpMafK is 2170 bp, which includes an open reading frame of 570 bp, encoding 190 amino acids...
March 15, 2018: Gene
David C Kieser, P J Cox, S C J Kieser
PURPOSE: Hirayama disease is an initially progressive disease caused by cervical neck flexion compressing the anterior horns of the lower cervical spinal cord. It is primarily seen in young males of Indian or Asian descent. With increasing dispersion of these populations this condition is increasingly being encountered internationally. This grand round reviews this rare but increasingly recognized condition. MATERIALS AND METHODS: We present a classic case of a young Indian male with progressive hand and forearm weakness...
March 14, 2018: European Spine Journal
Michael J Moses, Jared C Tishelman, Saqib Hasan, Peter L Zhou, Ioanna Zevgaras, Justin S Smith, Aaron J Buckland, Yong Kim, Afshin Razi, Themistocles S Protopsaltis
STUDY DESIGN: Cross-Sectional Study. OBJECTIVE: The goal of this study is to investigate how surgeons differ in collar and narcotic use, as well as return to driving recommendations following cervical spine surgeries and the associated medico-legal ramifications of these conditions. SUMMARY OF BACKGROUND DATA: Restoration of quality of life is one of the main goals of cervical spine surgery. Patients frequently inquire when they may safely resume driving after cervical spine surgery...
March 9, 2018: Spine
Jeavana Sritharan, Jill MacLeod, Shelley Harris, Donald C Cole, Anne Harris, Michael Tjepkema, Paul A Peters, Paul A Demers
As there are no well-established modifiable risk factors for prostate cancer, further evidence is needed on possible factors such as occupation. Our study uses one of the largest Canadian worker cohorts to examine occupation, industry, and prostate cancer and to assess patterns of prostate cancer rates. The Canadian Census Health and Environment Cohort (CanCHEC) was established by linking the 1991 Canadian Census Cohort to the Canadian Cancer Database (1969-2010), Canadian Mortality Database (1991-2011), and Tax Summary Files (1981-2011)...
March 1, 2018: Cancer Medicine
Eduarda Schultze, Tiago Collares, Caroline Lucas, Fabiana K Seixas
All-trans retinoic acid (ATRA), a derivative of vitamin A, has been shown to potentiate cancer chemotherapy due to its ability to induce signals for cell differentiation or death, and inhibit cell proliferation. The combination of ATRA with taxoids, kinase inhibitors, natural compounds, retinoids, ER or HER2 inhibitors, chemotherapeutic drugs, proteasome inhibitors and nanoformulations of tretinoin have demonstrated additive or synergistic effects in anti-cancer activities. The mechanisms by which the compounds exert their synergistic effects depend on the tumor and the cell type...
February 16, 2018: Chemico-biological Interactions
Samuel C Overley, Robert K Merrill, Evan O Baird, Joshua J Meaike, Samuel K Cho, Andrew C Hecht, Sheeraz A Qureshi
Study Design: Prospective randomized control trial. Objective: To investigate the role of cervical collars in postoperative care following 1- and 2-level instrumented anterior cervical discectomy and fusion (ACDF). Methods: The Cervical Spine Research Society Resident Fellow Grant funded this project. Fifty consecutive patients undergoing 1- or 2-level ACDF surgery were randomized into groups receiving either no brace or a cervical brace for 6 weeks postoperatively...
February 2018: Global Spine Journal
Karen K Klyczek, Deborah Jacobs-Sera, Tamarah L Adair, Sandra D Adams, Sarah L Ball, Robert C Benjamin, J Alfred Bonilla, Caroline A Breitenberger, Charles J Daniels, Bobby L Gaffney, Melinda Harrison, Lee E Hughes, Rodney A King, Gregory P Krukonis, A Javier Lopez, Kirsten Monsen-Collar, Marie C Pizzorno, Claire A Rinehart, Amanda K Staples, Emily L Stowe, Rebecca A Garlena, Daniel A Russell, Steven G Cresawn, Welkin H Pope, Graham F Hatfull
We report here the complete genome sequences of 44 phages infecting Arthrobacter sp. strain ATCC 21022. These phages have double-stranded DNA genomes with sizes ranging from 15,680 to 70,707 bp and G+C contents from 45.1% to 68.5%. All three tail types (belonging to the families Siphoviridae , Myoviridae , and Podoviridae ) are represented.
February 1, 2018: Genome Announcements
K F Coble, J M DeRouchey, M D Tokach, S S Dritz, R D Goodband, J C Woodworth
Two experiments were conducted to determine the duration of high-fiber ingredient removal from finishing pig diets before marketing to restore carcass yield and carcass fat iodine value (IV), similar to pigs continuously fed a corn-soybean meal diet. In Exp. 1, 288 pigs (initially 38.4 ± 0.3 kg BW) were used in an 88-d study and fed either a low-fiber corn-soybean meal diet from d 0 to 88, or a high-fiber diet containing 30% corn distillers dried grains with solubles and 19% wheat middlings until 20, 15, 10, 5, or 0 d before slaughter and switched to the low-fiber corn-soybean meal diet thereafter...
February 8, 2018: Journal of Animal Science
Raimunda Sâmia Nogueira Brilhante, Jonathas Sales de Oliveira, Antonio José de Jesus Evangelista, Vandbergue Santos Pereira, Lucas Pereira Alencar, Débora de Souza Collares Maia Castelo-Branco, Lília Maria Carneiro Câmara, Reginaldo Gonçalves de Lima-Neto, Rossana de Aguiar Cordeiro, José Júlio Costa Sidrim, Marcos Fábio Gadelha Rocha
The aim of this study was to evaluate the effect of promethazine on the antifungal minimum inhibitory concentrations against planktonic cells and mature biofilms of Candida tropicalis, as well as investigate its potential mechanisms of cell damage against this yeast species. Three C. tropicalis isolates (two azole-resistant and one azole-susceptible) were evaluated for their planktonic and biofilm susceptibility to promethazine alone and in combination with itraconazole, fluconazole, voriconazole, amphotericin B, and caspofungin...
October 9, 2017: Medical Mycology: Official Publication of the International Society for Human and Animal Mycology
Ângela Sena-Lopes, Francisco Silvestre Brilhante Bezerra, Raquel Nascimento das Neves, Rodrigo Barros de Pinho, Mara Thais de Oliveira Silva, Lucielli Savegnago, Tiago Collares, Fabiana Seixas, Karine Begnini, João Antonio Pêgas Henriques, Mariana Roesch Ely, Luciane C Rufatto, Sidnei Moura, Thiago Barcellos, Francine Padilha, Odir Dellagostin, Sibele Borsuk
Most studies of Brazilian red propolis have explored the composition and biological properties of its ethanolic extracts. In this work, we chemically extracted and characterized the essential oil of Brazilian red propolis (EOP) and assessed its adjuvant, antiparasitic and cytotoxic activities. The chemical composition of EOP was analyzed using gas chromatography with mass spectrometry (GC-MS). EOP was tested for in vitro activity against Trichomonas vaginalis (ATCC 30236 isolate); trophozoites were treated with different concentrations of EOP (ranging from 25 to 500 μg/mL) in order to establish the MIC and IC50 values...
2018: PloS One
Arnaud Metlaine, Fabien Sauvet, Danielle Gomez-Merino, Thierry Boucher, Maxime Elbaz, Jean Yves Delafosse, Damien Leger, Mounir Chennaoui
Professional burnout syndrome has been described in association with insomnia and metabolic, inflammatory and immune correlates. We investigated the interest of exploring biological parameters and sleep disturbances in relation to burnout symptoms among white-collar workers. Fifty-four participants with burnout were compared to 86 healthy control participants in terms of professional rank level, sleep, job strain (Karasek questionnaire), social support, anxiety and depression (HAD scale). Fasting concentrations of glycaemia, glycosylated hemoglobin (HbA1C), total-cholesterol, triglycerides, C-reactive protein (CRP), thyroid stimulating hormone (TSH), 25-hydroxyvitamin D (25[OH]D), and white blood cell (WBC) counts were assessed...
2018: PloS One
Jackie Eagers, Richard C Franklin, Matthew K Yau, Kieran Broome
BACKGROUND: The transition from work to retirement is a complex process and unique experience with a relationship existing between work and retirement with implications for health in later life. This review explored the relationship between pre-retirement job and participation in the work-to-retirement transition process in Australia by exploring: (i) factors influencing retirement in relation to pre-retirement job; (ii) how jobs are classified; (iii) the effect of pre-retirement job (based on categories) on this occupational transition; and (iv) the potential role for occupational therapy in this occupational transition...
January 24, 2018: Australian Occupational Therapy Journal
Susanne C Tonnon, Rozan van der Veen, Anja Th C M de Kruif, Suzan J W Robroek, Hidde P van der Ploeg, Karin I Proper, Allard J van der Beek
BACKGROUND: The aging work force makes sustainable employability (SE) of workers a priority. However, it is unknown to what extent employees use implemented SE measures. OBJECTIVE: To determine the utilization of 1) SE measures offered by employers, 2) employee SE strategies, and 3) to identify barriers and facilitators of SE strategies. METHODS: Survey data were collected among 731 blue collar and 879 white collar workers to determine the utilization of employer SE measures...
January 19, 2018: Work: a Journal of Prevention, Assessment, and Rehabilitation
Calogero C Tedesco, Fabrizio Veglia, Ulf de Faire, Sudhir Kurl, Andries J Smit, Rainer Rauramaa, Philippe Giral, Mauro Amato, Alice Bonomi, Alessio Ravani, Beatrice Frigerio, Samuela Castelnuovo, Daniela Sansaro, Elmo Mannarino, Steve E Humphries, Anders Hamsten, Elena Tremoli, Damiano Baldassarre
BACKGROUND AND AIMS: We aimed to examine the association between socioeconomic status (SES) and subclinical atherosclerosis, as assessed by carotid intima-media-thickness (C-IMT) and to investigate whether the effect of social inequality on C-IMT is mediated by cardiovascular (CV) risk factors and whether it is dissimilar in men and women, and in different European countries. METHODS: We assessed the association of lifelong occupation and educational level with C-IMT in the IMPROVE study cohort including 3703 subjects (median age 64...
December 20, 2017: Atherosclerosis
Xiaoying Zhou, Bin Wang, Jillian M Emerson, Carol S Ringelberg, Scott A Gerber, Jennifer J Loros, Jay C Dunlap
Circadian clocks are ubiquitous in eukaryotic organisms where they are used to anticipate regularly occurring diurnal and seasonal environmental changes. Nevertheless, little is known regarding pathways connecting the core clock to its output pathways. Here, we report that the HAD family phosphatase CSP-6 is required for overt circadian clock output but not for the core oscillation. The loss of function Δcsp-6 deletion mutant is overtly arrhythmic on race tubes under free running conditions; however, reporter assays confirm that the FREQUENCY-WHITE COLLAR COMPLEX core circadian oscillator is functional, indicating a discrete block between oscillator and output...
January 19, 2018: PLoS Genetics
E G Lopes, A P Sevá, F Ferreira, C M Nunes, L B Keid, R M Hiramoto, H L Ferreira, T M F S Oliveira, F G Ovallos, E A B Galati, T J Villegas, D V Bortoletto, S Y O B Valadas, R M Soares
Although a national programme for control of visceral leishmaniosis (VL) is being run in Brazil, the disease continues to spread. This programme is essentially based on culling infected dogs from endemic regions. Thus, there is an urgent need to develop other control measures against VL to deter its advance. Here, a subunit vaccine, a recombinant vaccine, an insecticide-impregnated collar and the associations between these measures were evaluated for reducing the incidence of Leishmania infection in dogs. This was through a cohort study conducted in an endemic region of Brazil, considering the incidence and time of total exposure over a period of 1 year...
January 18, 2018: Epidemiology and Infection
Ying He, Tonya L Taylor, Kiril M Dimitrov, Salman L Butt, James B Stanton, Iryna V Goraichuk, Heather Fenton, Rebecca Poulson, Jian Zhang, Corrie C Brown, Hon S Ip, Marcos Isidoro-Ayza, Claudio L Afonso
BACKGROUND: Newcastle disease viruses (NDV) are highly contagious and cause disease in both wild birds and poultry. A pigeon-adapted variant of genotype VI NDV, often termed pigeon paramyxovirus 1, is commonly isolated from columbids in the United States and worldwide. Complete genomic characterization of these genotype VI viruses circulating in wild columbids in the United States is limited, and due to the genetic variability of the virus, failure of rapid diagnostic detection has been reported...
January 12, 2018: Virology Journal
S A Barth, S Blome, D Cornelis, J Pietschmann, M Laval, O Maestrini, L Geue, F Charrier, E Etter, C Menge, M Beer, F Jori
On the Mediterranean island of Corsica, cohabitation between sympatric domestic pigs and Eurasian wild boar (Sus scrofa) is common and widespread and can facilitate the maintenance and dissemination of several pathogens detrimental for the pig industry or human health. In this study, we monitored a population of free-ranging domestic pigs reared in extensive conditions within a 800-ha property located in Central Corsica which was frequently visited by a sympatric population of wild boar between 2013 and 2015...
January 10, 2018: Transboundary and Emerging Diseases
Duarte S Viana, José Enrique Granados, Paulino Fandos, Jesús M Pérez, Francisco Javier Cano-Manuel, Daniel Burón, Guillermo Fandos, María Ángeles Párraga Aguado, Jordi Figuerola, Ramón C Soriguer
Background: Space use by animals is determined by the interplay between movement and the environment, and is thus mediated by habitat selection, biotic interactions and intrinsic factors of moving individuals. These processes ultimately determine home range size, but their relative contributions and dynamic nature remain less explored. We investigated the role of habitat selection, movement unrelated to habitat selection and intrinsic factors related to sex in driving space use and home range size in Iberian ibex, Capra pyrenaica...
2018: Movement Ecology
Xuhui Zhou, Xia Xu, Guiyao Zhou, Yiqi Luo
Temperature sensitivity of soil organic carbon (SOC) decomposition is one of the major uncertainties in predicting climate-carbon (C) cycle feedback. Results from previous studies are highly contradictory with old soil C decomposition being more, similarly, or less sensitive to temperature than decomposition of young fractions. The contradictory results are partly from difficulties in distinguishing old from young SOC and their changes over time in the experiments with or without isotopic techniques. In this study, we have conducted a long-term field incubation experiment with deep soil collars (0-70 cm in depth, 10 cm in diameter of PVC tubes) for excluding root C input to examine apparent temperature sensitivity of SOC decomposition under ambient and warming treatments from 2002 to 2008...
January 3, 2018: Global Change Biology
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