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https://www.readbyqxmd.com/read/28218268/rapid-and-sensitive-diagnoses-of-dry-root-rot-pathogen-of-chickpea-rhizoctonia-bataticola-taub-butler-using-loop-mediated-isothermal-amplification-assay
#1
Raju Ghosh, Avijit Tarafdar, Mamta Sharma
Dry root rot (DRR) caused by the fungus Rhizoctonia bataticola (Taub.) Butler, is an emerging disease in chickpea. The disease is often mistaken with other root rots like Fusarium wilt, collar rot and black root rot in chickpea. Therefore, its timely and specific detection is important. Current detection protocols are either based on mycological methods or on protocols involving DNA amplification by polymerase chain reaction (PCR). Here we report the rapid and specific detection of R. bataticola using loop-mediated isothermal amplification (LAMP) assay targeting fungal specific 5...
February 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28217398/comparison-of-intubation-success-and-glottic-visualization-using-king-vision-and-c-mac-videolaryngoscopes-in-patients-with-cervical-spine-injuries-with-cervical-immobilization-a-randomized-clinical-trial
#2
Dhanyasi Shravanalakshmi, Prasanna U Bidkar, K Narmadalakshmi, Suman Lata, Sandeep K Mishra, S Adinarayanan
BACKGROUND: Glottic visualization can be difficult with cervical immobilization in patients with cervical spine injury. Indirect laryngoscopes may provide better glottic visualization in these groups of patients. Hence, we compared King Vision videolaryngoscope, C-MAC videolaryngoscope for endotracheal intubation in patients with proven/suspected cervical spine injury. METHODS: After standard induction of anesthesia, 135 patients were randomized into three groups: group C (conventional C-MAC videolaryngoscope), group K (King Vision videolaryngoscope), and group D (D blade C-MAC videolaryngoscope)...
2017: Surgical Neurology International
https://www.readbyqxmd.com/read/28216009/upregulating-nrf2-dependent-antioxidant-defenses-in-pacific-oysters-crassostrea-gigas-investigating-the-nrf2-keap1-pathway-in-bivalves
#3
Naissa Maria Danielli, Rafael Trevisan, Danielle Ferraz Mello, Kelvis Fischer, Vanessa Schadeck Deconto, Daiane da Silva Acosta, Adalto Bianchini, Afonso C D Bainy, Alcir Luiz Dafre
Analysis of the Pacific oyster Crassostrea gigas annotated genome revealed genes with conserved sequences belonging to typical cap 'n' collar Nrf2 domain, a major player in antioxidant protection, and domains belonging to Nrf2 cytoplasmic repressor (Keap1), but little is known about Nrf2/Keap1 induction in bivalves. C. gigas were exposed to waterborne 10 and 30μM curcumin, a known inducer of the mammalian Nrf2. Curcumin disappeared from the seawater after 10h, and accumulated in the gills (10h) and digestive gland (10-96h)...
February 12, 2017: Comparative Biochemistry and Physiology. Toxicology & Pharmacology: CBP
https://www.readbyqxmd.com/read/28177378/shelter-type-and-birth-number-influence-the-birth-and-death-sites-of-lambs-and-ewe-movement-around-lambing-time
#4
J C Broster, R L Dehaan, D L Swain, S M Robertson, B J King, M A Friend
A significant number of lambs born each yr in Australia die within 72 h of birth. Periods of high wind, combined with rain and low temperatures, can lead to marked increases in the level of mortality. Under these weather conditions mortality levels may be reduced with the provision of shelter, provided it is utilized by lambs. This study used GPS collars to determine the use of shelter by ewes and lambs, to compare the movement of ewes with twin lambs across 2 types of shelter (hedgerows and shrubs), while also comparing ewes with single and twin lambs in a single shelter type (hedgerows)...
January 2017: Journal of Animal Science
https://www.readbyqxmd.com/read/28141768/atlantoaxial-rotatory-subluxation-in-children
#5
Elizabeth C Powell, Jeffrey R Leonard, Cody S Olsen, David M Jaffe, Jennifer Anders, Julie C Leonard
OBJECTIVES: Pediatric cervical injuries are uncommon. This study was to describe injury circumstances, clinical findings, and management among children diagnosed with atlantoaxial rotatory subluxation (AARS) to aid in its recognition and management. METHODS: Subanalysis of a large case-control study from January 2000 to December 2004 in 17 hospitals in the Pediatric Emergency Care Applied Research Network was performed. Cases were children younger than 16 years with AARS after blunt trauma (n = 55); controls were (a) children with other cervical spine injuries (other CSI, n = 485) and (b) those with normal imaging of the cervical spine (non-CSI, n = 1060)...
February 2017: Pediatric Emergency Care
https://www.readbyqxmd.com/read/28138819/asymmetric-competition-for-nectar-between-a-large-nectar-thief-and-a-small-pollinator-an-energetic-point-of-view
#6
Eliška Padyšáková, Jan Okrouhlík, Mark Brown, Michael Bartoš, Štěpán Janeček
There are two alternative hypotheses related to body size and competition for restricted food sources. The first one supposes that larger animals are superior competitors because of their increased feeding abilities, whereas the second one assumes superiority of smaller animals because of their lower food requirements. We examined the relationship between two unrelated species of different size, drinking technique, energy requirements and roles in plant pollination system, to reveal the features of their competitive interaction and mechanisms enabling their co-existence while utilising the same nectar source...
January 30, 2017: Oecologia
https://www.readbyqxmd.com/read/28135606/hla-g-variability-and-haplotypes-detected-by-massively-parallel-sequencing-procedures-in-the-geographicaly-distinct-population-samples-of-brazil-and-cyprus
#7
Erick C Castelli, Petroula Gerasimou, Michelle A Paz, Jaqueline Ramalho, Iane O P Porto, Thálitta H A Lima, Andréia S Souza, Luciana C Veiga-Castelli, Cristhianna V A Collares, Eduardo A Donadi, Celso T Mendes-Junior, Paul Costeas
The HLA-G molecule presents immunomodulatory properties that might inhibit immune responses when interacting with specific Natural Killer and T cell receptors, such as KIR2DL4, ILT2 and ILT4. Thus, HLA-G might influence the outcome of situations in which fine immune system modulation is required, such as autoimmune diseases, transplants, cancer and pregnancy. The majority of the studies regarding the HLA-G gene variability so far was restricted to a specific gene segment (i.e., promoter, coding or 3' untranslated region), and was performed by using Sanger sequencing and probabilistic models to infer haplotypes...
March 2017: Molecular Immunology
https://www.readbyqxmd.com/read/28100212/the-effects-of-breakfast-on-short-term-cognitive-function-among-chinese-white-collar-workers-protocol-for-a-three-phase-crossover-study
#8
Zhenchuang Tang, Na Zhang, Ailing Liu, Dechun Luan, Yong Zhao, Chao Song, Guansheng Ma
BACKGROUND: As the first meal of the day, breakfast plays an important role in supplying energy and nutrients, which are critical to working and learning activities. A three-phase crossover study was designed to investigate the effects of breakfast on cognitive function among Chinese white-collar workers. The planned study protocol is presented. METHODS: A total of 264 participants aged 25-45 years will be recruited from Shenyang and Chongqing. Self-administered questionnaires will be used to collect information on age, gender, marital status, education level, occupation, smoking habits, drinking habits, and breakfast behaviours...
January 18, 2017: BMC Public Health
https://www.readbyqxmd.com/read/28073868/delayed-diagnosis-of-fractured-anterior-arch-of-the-atlas-in-a-young-child
#9
Sergio B Sesia, Friederike Prüfer, Michael Walther, Daniel Studer
A 2-year-old girl fell off a bunk bed onto a parquet floor. She immediately reported neck pain and presented with muscle spasm and limited motion of the cervical spine (C-spine). Plain X-rays of the C-spine showed no osseous lesion. Owing to persisting pain and limited motion in the neck, MRI of the C-spine was obtained which revealed intact ligaments and cervical spinal cord, as well as soft tissue swelling in front of the anterior arch of the atlas. Subsequent CT of the C-spine confirmed a complete, undisplaced fracture of the anterior arch of the atlas (Gehweiler type I fracture)...
January 10, 2017: BMJ Case Reports
https://www.readbyqxmd.com/read/28056454/collar-sign-in-incompletely-occluded-aneurysms-after-pipeline-embolization-evaluation-with-angiography-and-optical-coherence-tomography
#10
C J Griessenauer, R Gupta, S Shi, A Alturki, R Motiei-Langroudi, N Adeeb, C S Ogilvy, A J Thomas
BACKGROUND AND PURPOSE: Flow diversion with the Pipeline Embolization Device has emerged as an attractive treatment for cerebral aneurysms. Processes involved in aneurysm occlusion include changes in intra-aneurysmal hemodynamics and endothelialization of the device. Here, we call attention to a radiographic sign not previously reported that is detected in incompletely occluded aneurysms after treatment with the Pipeline Embolization Device at angiographic follow-up and referred to as the "collar sign...
November 10, 2016: AJNR. American Journal of Neuroradiology
https://www.readbyqxmd.com/read/28041757/comparison-of-the-macintosh-laryngoscope-and-blind-intubation-via-the-igel-for-intubation-with-cervical-spine-immobilization-a-randomized-crossover-manikin-trial
#11
Pawel Gawlowski, Jacek Smereka, Marcin Madziala, Lukasz Szarpak, Michael Frass, Oliver Robak
INTRODUCTION: Endotracheal intubation (ETI) using a Macintosh laryngoscope (MAC) requires the head to be positioned in a modified Jackson position, slightly reclined and elevated. Intubation of trauma patients with an injured neck or spine is therefore difficult, since the neck usually cannot be turned or is already immobilized in order to prevent further injury. The iGEL supraglottic airway seems optimal for such conditions due to its blind insertion without the need of a modified Jackson position...
November 30, 2016: American Journal of Emergency Medicine
https://www.readbyqxmd.com/read/28030568/space-use-and-movement-of-a-neotropical-top-predator-the-endangered-jaguar
#12
Ronaldo G Morato, Jared A Stabach, Chris H Fleming, Justin M Calabrese, Rogério C De Paula, Kátia M P M Ferraz, Daniel L Z Kantek, Selma S Miyazaki, Thadeu D C Pereira, Gediendson R Araujo, Agustin Paviolo, Carlos De Angelo, Mario S Di Bitetti, Paula Cruz, Fernando Lima, Laury Cullen, Denis A Sana, Emiliano E Ramalho, Marina M Carvalho, Fábio H S Soares, Barbara Zimbres, Marina X Silva, Marcela D F Moraes, Alexandre Vogliotti, Joares A May, Mario Haberfeld, Lilian Rampim, Leonardo Sartorello, Milton C Ribeiro, Peter Leimgruber
Accurately estimating home range and understanding movement behavior can provide important information on ecological processes. Advances in data collection and analysis have improved our ability to estimate home range and movement parameters, both of which have the potential to impact species conservation. Fitting continuous-time movement model to data and incorporating the autocorrelated kernel density estimator (AKDE), we investigated range residency of forty-four jaguars fit with GPS collars across five biomes in Brazil and Argentina...
2016: PloS One
https://www.readbyqxmd.com/read/27984785/comparison-of-n-and-o-linked-glycosylation-patterns-of-ebolavirus-glycoproteins
#13
Amanda L Collar, Elizabeth C Clarke, Eduardo Anaya, Denise Merrill, Sarah Yarborough, Scott M Anthony, Jens H Kuhn, Christine Merle, Manfred Theisen, Steven B Bradfute
Ebolaviruses are emerging pathogens that cause severe and often fatal viral hemorrhagic fevers. Four distinct ebolaviruses are known to cause Ebola virus disease in humans. The ebolavirus envelope glycoprotein (GP1,2) is heavily glycosylated, but the precise glycosylation patterns of ebolaviruses are largely unknown. Here we demonstrate that approximately 50 different N-glycan structures are present in GP1,2 derived from the four pathogenic ebolaviruses, including high mannose, hybrid, and bi-, tri-, and tetra-antennary complex glycans with and without fucose and sialic acid...
February 2017: Virology
https://www.readbyqxmd.com/read/27942178/white-collar-rhabdomyolysis-with-acute-kidney-injury
#14
R K Bhakthavatsalam, G Venu, P Krishnam Raju, H C Madhusudan
Rhabdomyolysis is a clinical syndrome resulting from the disintegration of muscle cell and spillage of toxic intracellular contents into circulation. Strenuous, unaccustomed exercise leads to exertional rhabdomyolysis and cause AKI. We report a 26-year-old female who developed white collar rhabdomyolysis with AKI after performing sit-ups (Super Yoga Brain) for 108 times in temple. She was managed with hemodialysis and supporting therapy. She made a full recovery after 4 weeks. Awareness of this condition and early diagnosis is highlighted...
November 2016: Indian Journal of Nephrology
https://www.readbyqxmd.com/read/27933209/prevalence-and-incidence-of-gout-in-southern-sweden-from-the-socioeconomic-perspective
#15
Meliha C Kapetanovic, Mohaned Hameed, Aleksandra Turkiewicz, Tuhina Neogi, Tore Saxne, Lennart Jacobsson, Martin Englund
OBJECTIVES: To estimate the prevalence and cumulative incidence of gout in southern Sweden with respect to socioeconomic status. METHODS: Among residents of Skåne region in the year 2013 (total population 1.3 million), adult persons (age 18 years +) who between 1998 and 2013 received a diagnosis of gout (International Classification of Disease 10th Edition (ICD-10) code M10) by any physician were identified using the Skåne Healthcare Register. We calculated the point prevalence by end of 2013 and annual cumulative incidence in 2013 standardised to the whole Skåne population according to sex, individual information on occupation (white collar/blue collar), income (low/middle/high) and level of education (primary school/high school/university)...
2016: RMD Open
https://www.readbyqxmd.com/read/27933063/aire-downregulation-is-associated-with-changes-in-the-posttranscriptional-control-of-peripheral-tissue-antigens-in-medullary-thymic-epithelial-cells
#16
Ernna H Oliveira, Claudia Macedo, Cristhianna V Collares, Ana Carolina Freitas, Paula Barbim Donate, Elza T Sakamoto-Hojo, Eduardo A Donadi, Geraldo A Passos
Autoimmune regulator (Aire) is a transcriptional regulator of peripheral tissue antigens (PTAs) and microRNAs (miRNAs) in medullary thymic epithelial cells (mTECs). In this study, we tested the hypothesis that Aire also played a role as an upstream posttranscriptional controller in these cells and that variation in its expression might be associated with changes in the interactions between miRNAs and the mRNAs encoding PTAs. We demonstrated that downregulation of Aire in vivo in the thymuses of BALB/c mice imbalanced the large-scale expression of these two RNA species and consequently their interactions...
2016: Frontiers in Immunology
https://www.readbyqxmd.com/read/27931926/evaluating-wildlife-cattle-contact-rates-to-improve-the-understanding-of-dynamics-of-bovine-tuberculosis-transmission-in-michigan-usa
#17
Michael J Lavelle, Shannon L Kay, Kim M Pepin, Daniel A Grear, Henry Campa, Kurt C VerCauteren
Direct and indirect contacts among individuals drive transmission of infectious disease. When multiple interacting species are susceptible to the same pathogen, risk assessment must include all potential host species. Bovine tuberculosis (bTB) is an example of a disease that can be transmitted among several wildlife species and to cattle, although the potential role of several wildlife species in spillback to cattle remains unclear. To better understand the complex network of contacts and factors driving disease transmission, we fitted proximity logger collars to beef and dairy cattle (n=37), white-tailed deer (Odocoileus virginianus; n=29), raccoon (Procyon lotor; n=53), and Virginia opossum (Didelphis virginiana; n=79) for 16 months in Michigan's Lower Peninsula, USA...
December 1, 2016: Preventive Veterinary Medicine
https://www.readbyqxmd.com/read/27928446/gastrostomy-with-peritoneal-collar-versus-percutaneous-endoscopic-gastrostomy
#18
Tudor C, Branescu C, Savlovschi C, El-Khatib A, Pantu H, Nica A, Dascalu Am, Masoumeh B, Tudor As, Oprescu Sm, Serban D
Aim. The present study aimed to perform a medico-surgical comparative analysis of the 2 most widely used techniques: gastrostomy with peritoneal collar versus percutaneous endoscopic gastrostomy, based on the vast clinical experience in an Upper Digestive Surgery Clinic. Materials and method. A retrospective study was carried out between January 2010 and January 2015 on the patients admitted for a surgical solution for feeding. The indications, preoperative preparation, surgical techniques, and postoperative outcomes were analyzed...
October 2016: Journal of Medicine and Life
https://www.readbyqxmd.com/read/27925064/does-hair-coat-length-affect-flea-infestation-in-naturally-infested-dogs
#19
Guilherme Araujo Collares da Silva, Luciana Araujo Lins, Márcio Josué Costa Irala, Marcial Corrêa Cárcamo, Paulo Bretanha Ribeiro
The Siphonaptera are parasitic insects of endothermic animals and Ctenocephalides felis and Pulex irritans are important parasites of dogs. This study evaluated the effect of hair coat length and time of year on the population size of C. felis and P. irritans in naturally infested dogs. Fleas were collected from 14 dogs on a monthly basis for a year (February 2015 to January 2016) at a rural property in Bagé, Rio Grande do Sul, Brazil. The dogs were divided into two groups based on hair coat length: short coat (coat length < 5...
October 2016: Revista Brasileira de Parasitologia Veterinária, Brazilian Journal of Veterinary Parasitology
https://www.readbyqxmd.com/read/27918863/ems-providers-beliefs-regarding-spinal-precautions-for-pediatric-trauma-transport
#20
Cindy D Chang, Remle P Crowe, Melissa A Bentley, Alyssa R Janezic, Julie C Leonard
OBJECTIVE: Describe prehospital Emergency Medical Services (EMS) providers' beliefs regarding spinal precautions for pediatric trauma transport. METHODS: We randomly surveyed nationally certified EMS providers. We assessed providers' beliefs about specific precautions, and preferred precautions given a child's age (0-4 or 5-18 years) and presence of specific cervical spine injury (CSI) risk factors. RESULTS: We received 5,400 responses (17%)...
December 5, 2016: Prehospital Emergency Care
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