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P. gingivalis

Riyoko Tamai, Michiyo Kobayashi-Sakamoto, Yusuke Kiyoura
Galectin-1 and galectin-3 are C-type lectin receptors that bind to lipopolysaccharide (LPS) in the cell wall of gram-negative bacteria. In this study, we investigated the effects of galectin-1 and galectin-3 on adhesion to and invasion of the human gingival epithelial cell line Ca9-22 by Porphyromonas gingivalis, a periodontal pathogenic gram-negative bacterium. Recombinant galectin-1, but not galectin-3, enhanced P. gingivalis adhesion and invasion, although both galectins bound similarly to P. gingivalis...
March 15, 2018: Canadian Journal of Microbiology
Monica Raina, Amber M Bates, Carol L Fischer, Ann Progulske-Fox, Taher Abbasi, Shireen Vali, Kim A Brogden
BACKGROUND: Matrix metalloproteinases (MMPs) are zinc- or calcium-dependent proteinases involved in normal maintenance of extracellular matrix. When elevated, they contribute to the tissue destruction seen in periodontal disease. Recently, we found that human beta defensin 3 (HBD3), a cationic antimicrobial peptide, alters chemokine and proinflammatory cytokine responses in human myeloid dendritic cells exposed to Porphyromonas gingivalis hemagglutinin B (HagB). In this study, the hypotheses that HagB induces MMP production in dendritic cells and that HBD3 mixed with HagB prior to treatment alters HagB-induced MMP profiles were tested...
March 2018: Journal of Periodontology
Lazaros I Sakkas, Dimitrios P Bogdanos
PURPOSE OF REVIEW: This review highlights the most recent data obtained in this field and provides clues toward the better understanding of the close interplay between microbiota and host, leading to autoimmune diseases. RECENT FINDINGS: A well-described model of microbiota/host interaction of relevance to autoimmunity is that linking anti-citrullinated peptide antibody positive rheumatoid arthritis and alterations of microbiota largely concentrating on Porphyromonas gingivalis and more recently of Aggregatibacter actinomycetemcomitans and Prevotella copri...
March 13, 2018: Current Opinion in Rheumatology
Hyun-Joo Kim, Gil Sun Cha, Hyung-Joon Kim, Eun-Young Kwon, Ju-Youn Lee, Jeomil Choi, Ji-Young Joo
Purpose: The aim of this study was to evaluate the ability of Porphyromonas gingivalis ( P. gingivalis ) to induce oxidation of high-density lipoprotein (HDL) and to determine whether the oxidized HDL induced by P. gingivalis exhibited altered antiatherogenic function or became proatherogenic. Methods: P. gingivalis and THP-1 monocytes were cultured, and the extent of HDL oxidation induced by P. gingivalis was evaluated by a thiobarbituric acid-reactive substances (TBARS) assay...
February 2018: Journal of Periodontal & Implant Science
Yan Xu, Tshepiso Selerio-Poely, Xingru Ye
Purpose: To evaluate the clinical and microbiological effects of the local use of egg yolk immunoglobulin against Porphyromonas gingivalis (anti- P.g. IgY) as an adjunct to scaling and root planing (SRP) in the treatment of moderate to severe chronic periodontitis. Methods: This was a randomized, placebo-controlled, double-blind trial involving 60 systematically healthy patients with moderate to severe chronic periodontitis. Subjects (n=20/group) were randomly assigned to receive SRP combined with subgingival irrigation of anti- P...
February 2018: Journal of Periodontal & Implant Science
Lívia Jacovassi Tavares, Marlise Inêz Klein, Beatriz Helena Dias Panariello, Erica Dorigatti de Avila, Ana Cláudia Pavarina
Purpose: The goal of this study was to develop and validate a standardized in vitro pathogenic biofilm attached onto saliva-coated surfaces. Methods: Fusobacterium nucleatum (F. nucleatum) and Porphyromonas gingivalis (P. gingivalis) strains were grown under anaerobic conditions as single species and in dual-species cultures. Initially, the bacterial biomass was evaluated at 24 and 48 hours to determine the optimal timing for the adhesion phase onto saliva-coated polystyrene surfaces...
February 2018: Journal of Periodontal & Implant Science
Mizuki Sekiya, Yu Shimoyama, Taichi Ishikawa, Minoru Sasaki, Masamitsu Futai, Mayumi Nakanishi-Matsui
Porphyromonas gingivalis is a well-known Gram-negative bacterium that causes periodontal disease. The bacterium metabolizes amino acids and peptides to obtain energy. An ion gradient across its plasma membrane is thought to be essential for nutrient import. However, it is unclear whether an ion-pumping ATPase responsible for the gradient is required for bacterial growth. Here, we report the inhibitory effect of protonophores and inhibitors of a proton-pumping ATPase on the growth of P. gingivalis. Among the compounds examined, curcumin and citreoviridin appreciably reduced the bacterial growth...
March 9, 2018: Biochemical and Biophysical Research Communications
U Kandalam, N Ledra, H Laubach, K V Venkatachalam
BACKGROUND AND OBJECTIVES: Pathogenic infections caused by Porphyromonas gingivalis, Treponema denticola, and Tannerella forsythia can result in the production of volatile sulfur compounds (VSC's) and other toxic compounds from methionine catabolism that can lead to halitosis and periodontitis. Our aim is to block the activity of methionine gammalyase-deaminase (Mgld) of methionine catabolism to prevent halitosis/periodontitis. DESIGNS: Cloned, expressed, Mgld protein was tested for purity by SDS-PAGE and western blotting...
March 1, 2018: Archives of Oral Biology
Seiya Kato, Daisuke Ekuni, Shigehisa Kawakami, Acing Habibie Mude, Manabu Morita, Shogo Minagi
OBJECTIVE: This study was conducted to investigate whether the masseter muscle activity shows any specific pattern in relation to the severity of periodontitis. DESIGN: Sixteen subjects with no or mild periodontitis (NMP group) and 15 subjects with moderate or severe periodontitis (MSP group) were enrolled. Plasma IgG antibody titer was examined using Porphyromonas gingivalis as a bacterial antigen. Surface electromyography (EMG) of the masseter muscles was continuously recorded using an ambulatory surface EMG recording device while patients were awake and asleep...
March 1, 2018: Archives of Oral Biology
Carina M Silva-Boghossian, Paola C Cesário, Anna Thereza T Leão, Ana Paula V Colombo
BACKGROUND: This study compared the composition of subgingival microbiota between obese and non-obese women with or without periodontal disease. METHODS: Full-mouth periodontal clinical assessments were carried out in 76 obese women (17 periodontally healthy and 59 with periodontal disease), and 34 non-obese women (12 periodontally healthy, 22 with periodontal disease). Subgingival biofilm samples were individually obtained from seven sites of each individual, and the prevalence and counts of 40 bacterial taxa were determined by the checkerboard method...
February 2018: Journal of Periodontology
Daniel Belstrøm, Maria Anastasia Grande, Maria Lynn Sembler-Møller, Nikolai Kirkby, Sean L Cotton, Bruce J Paster, Palle Holmstrup
BACKGROUND: The purpose of this study was to characterize and compare subgingival and salivary microbiotas before and after periodontal treatment to learn if any changes of the subgingival microbiota were reflected in saliva. We tested the hypothesis that salivary levels of specific periopathogens correlate with corresponding subgingival levels before and after periodontal treatment. METHODS: Twenty-five patients with generalized chronic periodontitis completed the study...
February 19, 2018: Journal of Periodontology
Hua Qi, Baosheng Li, Heling Wang, Qing Cai, Xu Quan, Yunxia Cui, Weiyan Meng
BACKGROUND: When presented with a surface or an interface, bacteria often grow as biofilms in which cells are held together by an extracellular matrix. Biofilm formation on implants is an initiating factor for their failure. Porphyromonas gingivalis is the primary etiologic bacteria of initiation and progression of periodontal disease. This microorganism is also the risk factor of many systemic diseases, such as cardiovascular disease, diabetes, and pulmonary infection. To date, no medication that can remove such biofilm has been accepted for clinical use...
March 2018: Journal of Periodontology
Jia Ni, Lei Chen, Sulan Zhong, Qiaoxue Chai, Lin Zhang, Dan Wang, Shaobing Li, Jincai Zhang
BACKGROUND: The aim of the study was to explore the influence of periodontitis and scaling and root planning on insulin resistance and hepatic CD36 in obese rats with periodontitis. METHODS: Thirty-two specific pathogen free Sprague-Dawley rats were randomly divided into four groups of eight animals each as follows: healthy rats (healthy group), obese rats (obesity group), obese rats with periodontitis (non-therapy group), and obese rats with periodontitis who underwent periodontal scaling and root planning (SRP) (therapy group)...
February 22, 2018: Journal of Periodontology
P Zhang, W Zhang, D Zhang, M Wang, R Aprecio, N Ji, O Mohamed, Y Li, Y Ding, Q Wang
BACKGROUND AND OBJECTIVE: Periodontitis is an increasingly prevalent complication of diabetes mellitus (known as diabetes mellitus-associated periodontitis), and 25-hydroxyvitamin D3 (25VD3 ) was recently found to be a critical regulator of innate immunity in this disease, but the underlying mechanisms remain poorly understood. T-cell protein tyrosine phosphatase non-receptor type 2 (PTPN2) is a potential downstream protein of the 25VD3 /vitamin D receptor pathway. The aim of this study was to investigate the regulation of PTPN2 in periodontal inflammation in diabetes mellitus-associated periodontitis...
March 8, 2018: Journal of Periodontal Research
Ahmad Al-Attar, Yelena Alimova, Sreenatha Kirakodu, Anastasia Kozal, Michael John Novak, Arnold J Stromberg, Luis Orraca, Janis Gonzalez-Martinez, Melween Martinez, Jeffrey L Ebersole, Octavio A Gonzalez
P. gingivalis (Pg) is an oral pathogen with the ability to induce oral dysbiosis and periodontal disease. Nevertheless, the mechanisms by which mucosal responses to the oral microbiota in the presence of specific pathogens such as Pg could abrogate the host-microbe symbiotic relationship leading to periodontitis remain unclear. Herein, we identified the Notch-1/PLA2 -IIA axis as a new molecular pathway through which Pg could be specifically modulating oral epithelial antimicrobial and inflammatory responses...
March 7, 2018: Mucosal Immunology
Giorgio Gabarrini, Laura M Palma Medina, Tim Stobernack, Rianne C Prins, Marines du Teil Espina, Jeroen Kuipers, Monika A Chlebowicz, John W A Rossen, Arie Jan van Winkelhoff, Jan Maarten van Dijl
The oral pathogen Porphyromonas gingivalis is one of the major periodontal agents and it has been recently hailed as a potential cause of the autoimmune disease rheumatoid arthritis. In particular, the peptidylarginine deiminase enzyme of P. gingivalis (PPAD) has been implicated in the citrullination of certain host proteins and the subsequent appearance of antibodies against citrullinated proteins, which might play a role in the etiology of rheumatoid arthritis. The aim of this study was to investigate the extracellular localization of PPAD in a large panel of clinical P...
January 1, 2018: Virulence
Norihiro Sonoi, Hiroshi Maeda, Toshimitsu Murauchi, Tadashi Yamamoto, Kazuhiro Omori, Susumu Kokeguchi, Koji Naruishi, Shogo Takashiba
An insertion sequence, IS1598 (IsPg4) has been found in virulent strains of Porphyromonas gingivalis in a murine abscess model. The present study was performed to investigate the effects of genetic rearrangements by IS1598 on the phenotypic characteristics of the virulent strains. For this purpose, we searched for a common insertion site of IS1598 among the virulent strains. Through cloning and database search, a common insertion site was identified beside an nrdD-like gene in the virulent FDC 381, W83 and W50 strains...
January 2018: New Microbiologica
Jiamin Aw, Glen M Scholz, Noorjahan Laila Huq, Jennifer Huynh, Neil M O'Brien-Simpson, Eric C Reynolds
Porphyromonas gingivalis is a keystone pathogen in chronic periodontitis. Its expression of gingipain proteases (Kgp and RgpA/B) is central to the stimulation of chronic inflammation. In this study, we investigated the inflammatory response of oral epithelial cells to P. gingivalis. The cells responded by upregulating the expression of the orphan chemokine CXCL14. The stimulation of CXCL14 expression was largely triggered by the gingipain proteases, and was dependent on the host protease-activated receptor PAR-3...
March 5, 2018: Cellular Microbiology
Ingar Olsen, Sim K Singhrao
Lipopolysaccharide (LPS) of Porphyromonas gingivalis exists in at least two known forms, O-LPS and A-LPS. A-LPS shows heterogeneity in which two isoforms designated LPS1 ,435/1 ,449 and LPS1 ,690 appear responsible for tissue-specific immune signalling pathways activation and increased virulence. The modification of lipid A to tetra-acylated1 ,435/1 ,449 and/or penta-acylated1 ,690 fatty acids indicates poor growth conditions and bioavailability of hemin. Hemin protects P. gingivalis from serum resistance and the lipid A serves as a site for its binding...
2018: Journal of Oral Microbiology
Martin Eckert, Danuta Mizgalska, Anton Sculean, Jan Potempa, Andreas Stavropoulos, Sigrun Eick
Porphyromonas gingivalis and Tannerella forsythia secrete proteases, gingipains and KLIKK-proteases. In addition, T. forsythia produces a serpin (miropin) with broad inhibitory spectrum. The aim of this pilot study was to determine the level of expression of miropin and individual proteases in vivo in periodontal and peri-implant health and disease conditions. Biofilm and gingival/peri-implant crevicular fluid (GCF or PISF respectively) samples from healthy tooth and implant sites (n=10), gingivitis and mucositis sites (n=12), and periodontitis and peri-implantitis sites (n=10)...
March 2, 2018: Molecular Oral Microbiology
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