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https://www.readbyqxmd.com/read/28635593/the-protein-slr1143-is-an-active-diguanylate-cyclase-in-synechocystis-sp-pcc-6803-and-interacts-with-the-photoreceptor-cph2
#1
Veronika Angerer, Philipp Schwenk, Thomas Wallner, Volkhard Kaever, Andreas Hiltbrunner, Annegret Wilde
Cyclic-di-GMP is an ubiquitous second messenger in bacteria. Several c-di-GMP receptor proteins have been identified to date, and downstream signalling pathways are often mediated through protein-protein interactions. The photoreceptor Cph2 from the cyanobacterium Synechocystis sp. PCC 6803 comprises three domains related to c-di-GMP metabolism: two GGDEF and one EAL domain. It has been shown that the C-terminal GGDEF domain acts as blue-light triggered c-di-GMP producer thereby inhibiting motility of the cells in blue light...
June 21, 2017: Microbiology
https://www.readbyqxmd.com/read/28632959/defect-based-modulation-of-opto-electronic-properties-for-bio-functionalized-hexagonal-boron-nitride-nanosheets
#2
Mehdi Shakourian-Fard, Hadiseh Heydari, Ganesh Kamath
Defect engineering could potentially allow for dramatic tuning of optoelectronic properties of two-dimensional materials. With the help of density functional theory (DFT) calculations, we present a systematic study of the DNA Nucleobases (NBs) adsorbed on hexagonal boron-nitride nanoflake (h-BNNF) and boron and nitrogen monovacancies. The presence of such VN and VB defects increases the binding strength of NBs by 9 kcal/mol and 34 kcal/mol, respectively (h-BNNF-VB> h-BNNF-VN> h-BNNF). A more negative electrostatic potential at the B vacancy-site makes h-BNNF-VB surface more reactive than h-BNNF-VN surface enabling H-bonding interactions with NBs...
June 20, 2017: Chemphyschem: a European Journal of Chemical Physics and Physical Chemistry
https://www.readbyqxmd.com/read/28631315/a-two-year-study-of-%C3%AE-9-tetrahydrocannabinol-concentrations-in-drivers-part-2-physiological-signs-on-drug-recognition-expert-dre-and-non-dre-examinations
#3
Kari Declues, Shelli Perez, Ariana Figueroa
Whole blood samples were examined for ∆(9) -Tetrahydrocannabinol (THC) over 2 years in drivers suspected of driving under the influence. Part one of the study examined the link between [THC] and performance on field sobriety tests. This portion examined objective signs, eye examinations and physiological indicators; and their relationship to the presence of THC. Several objective signs were excellent indicators of the presence of THC: red eyes (94%), droopy eyelids (85.6%), affected speech (87.6%), tongue coating (96...
June 20, 2017: Journal of Forensic Sciences
https://www.readbyqxmd.com/read/28630783/highly-sensitive-colorimetric-assay-for-determining-fe-3-based-on-gold-nanoparticles-conjugated-with-glycol-chitosan
#4
Kyungmin Kim, Yun-Sik Nam, Yeonhee Lee, Kang-Bong Lee
A highly sensitive and simple colorimetric assay for the detection of Fe(3+) ions was developed using gold nanoparticles (AuNPs) conjugated with glycol chitosan (GC). The Fe(3+) ion coordinates with the oxygen atoms of GC in a hexadentate manner (O-Fe(3+)-O), decreasing the interparticle distance and inducing aggregation. Time-of-flight secondary ion mass spectrometry showed that the bound Fe(3+) was coordinated to the oxygen atoms of the ethylene glycol in GC, which resulted in a significant color change from light red to dark midnight blue due to aggregation...
2017: Journal of Analytical Methods in Chemistry
https://www.readbyqxmd.com/read/28630286/efficient-switching-of-mcherry-fluorescence-using-chemical-caging
#5
Bas M C Cloin, Elke De Zitter, Desiree Salas, Vincent Gielen, Gert E Folkers, Marina Mikhaylova, Maike Bergeler, Bartosz Krajnik, Jeremy Harvey, Casper C Hoogenraad, Luc Van Meervelt, Peter Dedecker, Lukas C Kapitein
Fluorophores with dynamic or controllable fluorescence emission have become essential tools for advanced imaging, such as superresolution imaging. These applications have driven the continuing development of photoactivatable or photoconvertible labels, including genetically encoded fluorescent proteins. These new probes work well but require the introduction of new labels that may interfere with the proper functioning of existing constructs and therefore require extensive functional characterization. In this work we show that the widely used red fluorescent protein mCherry can be brought to a purely chemically induced blue-fluorescent state by incubation with β-mercaptoethanol (βME)...
June 19, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28628685/a-systematic-review-of-light-emitting-diode-led-phototherapy-for-treatment-of-psoriasis-an-emerging-therapeutic-modality
#6
Derek Ho, Eugene Koo, Andrew Mamalis, Jared Jagdeo
<p>Background: Psoriasis is a chronic, inflammatory skin condition. The economic burden of psoriasis is approximately $35.2 billion in the United States per year, and treatment costs are increasing at a higher rate than general inflation. Light emitting diode (LED) phototherapy may represent a cost-effective, efficacious, safe, and portable treatment modality for psoriasis.</p> <p>Objective: The goal of our manuscript is to review the published literature and provide evidence-based recommendations on LED phototherapy for the treatment of psoriasis...
May 1, 2017: Journal of Drugs in Dermatology: JDD
https://www.readbyqxmd.com/read/28628125/enhanced-insulin-sensitivity-in-successful-long-term-weight-loss-maintainers-compared-with-matched-controls-with-no-weight-loss-history
#7
L D Clamp, D J Hume, E V Lambert, J Kroff
BACKGROUND: Weight gain is associated with deterioration in metabolic health, whereas weight loss improves insulin sensitivity. This study assesses the impact of long-term, successfully maintained weight loss and weight-loss relapse on measures of insulin sensitivity and identifies factors that explain variability in insulin sensitivity. METHODS: Women (20-45 years) were recruited into four groups: reduced-overweight/obese (RED, n=15); body mass index (BMI)-matched controls (stable low-weight, n=19), BMI⩽27 kg m(-2); relapsed-overweight/obese subjects (REL, n=11); and BMI-matched controls (obese stable weight, n=11), BMI⩾27 kg m(-2)...
June 19, 2017: Nutrition & Diabetes
https://www.readbyqxmd.com/read/28627345/-effects-of-microrna-21-inhibitor-on-apoptosis-of-type-ii-alveolar-epithelial-cells-in-rats-with-hyperoxia-induced-acute-lung-injury
#8
Lei Shi, Ying He, Bing Bai, Miao Chen
OBJECTIVE: To observe the effects of microRNA-21 (miR-21) inhibitor on apoptosis of type II alveolar epithelial cells (AEC II) in rats with hyperoxia-induced acute lung injury (HALI). METHODS: Eighty Sprague-Dawley (SD) rats were divided into air-control group, hyperoxia injury group, empty-virus control group (200 μL solution with lentivirus was dropped into the nasal) and miR-21 inhibitor pretreatment group (200 μL solution with lentivirus contained miR-21 inhibitor was dropped through the nasal) by random number table...
March 2017: Zhonghua Wei Zhong Bing Ji Jiu Yi Xue
https://www.readbyqxmd.com/read/28625924/protoporphyrin-ix-fluorescence-as-potential-indicator-of-psoriasis-severity-and-progression
#9
Bo Wang, Yu-Ting Xu, Li Zhang, Jie Zheng, Ronald Sroka, Hong-Wei Wang, Xiu-Li Wang
BACKGROUND AND OBJECTIVES: In psoriatic lesions, fluorescence diagnosis with blue light can detect protoporphyrin IX accumulation, especially after topical 5-aminolaevulinic acid (ALA) application. However, variable fluorescence distributions, interpersonal variations and long incubation time limit its wide application in clinic. This study is aimed to identify a consistent and convenient method to facilitate diagnosis and evaluation of psoriatic lesions. METHODS: 104 psoriatic lesions from 30 patients were evaluated...
June 15, 2017: Photodiagnosis and Photodynamic Therapy
https://www.readbyqxmd.com/read/28623354/tomographic-particle-image-velocimetry-using-smartphones-and-colored-shadows
#10
Andres A Aguirre-Pablo, Meshal K Alarfaj, Er Qiang Li, J F Hernández-Sánchez, Sigurdur T Thoroddsen
We demonstrate the viability of using four low-cost smartphone cameras to perform Tomographic PIV. We use colored shadows to imprint two or three different time-steps on the same image. The back-lighting is accomplished with three sets of differently-colored pulsed LEDs. Each set of Red, Green & Blue LEDs is shone on a diffuser screen facing each of the cameras. We thereby record the RGB-colored shadows of opaque suspended particles, rather than the conventionally used scattered light. We subsequently separate the RGB color channels, to represent the separate times, with preprocessing to minimize noise and cross-talk...
June 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28622567/the-application-of-traffic-light-food-labelling-in%C3%A2-a%C3%A2-worksite-canteen-intervention-in-taiwan
#11
H-J Chen, S-H Weng, Y-Y Cheng, A Y Z Lord, H-H Lin, W-H Pan
OBJECTIVE: This study evaluated customer attitudes, perceptions, and utilisation of a traffic-light food labelling (TFL) programme before and after the TFL was implemented in a worksite canteen in Taiwan. STUDY DESIGN: A one-arm intervention was implemented in the canteen and buffet of a research park in Taiwan. Phase 1 consisted of dissemination of information regarding the TFL, targeting the customers (June-July, 2014); phase 2 consisted of implementation of the TFL in the buffet starting in August 2014...
June 13, 2017: Public Health
https://www.readbyqxmd.com/read/28622287/a-niche-for-cyanobacteria-producing-chlorophyll-f-within-a-microbial-mat
#12
Satoshi Ohkubo, Hideaki Miyashita
Acquisition of additional photosynthetic pigments enables photosynthetic organisms to survive in particular niches. To reveal the ecological significance of chlorophyll (Chl) f, we investigated the distribution of Chl and cyanobacteria within two microbial mats. In a 7-mm-thick microbial mat beneath the running water of the Nakabusa hot spring, Japan, Chl f was only distributed 4.0-6.5 mm below the surface, where the intensity of far-red light (FR) was higher than that of photosynthetically active radiation (PAR)...
June 16, 2017: ISME Journal
https://www.readbyqxmd.com/read/28621997/cooling-a-harmonic-oscillator-by-optomechanical-modification-of-its-bath
#13
Xunnong Xu, Thomas Purdy, Jacob M Taylor
Optomechanical systems show tremendous promise for the high-sensitivity sensing of forces and modification of mechanical properties via light. For example, similar to neutral atoms and trapped ions, laser cooling of mechanical motion by radiation pressure can take single mechanical modes to their ground state. Conventional optomechanical cooling is able to introduce an additional damping channel to mechanical motion while keeping its thermal noise at the same level, and, as a consequence, the effective temperature of the mechanical mode is lowered...
June 2, 2017: Physical Review Letters
https://www.readbyqxmd.com/read/28621945/concentration-driven-selectivity-of-energy-transfer-channels-and-color-tunability-in-ba3la-po4-3-tb-3-sm-3-for-warm-white-leds
#14
Weijie Zhou, Meng Gu, Yiyi Ou, Caihua Zhang, Xuejie Zhang, Lei Zhou, Hongbin Liang
Here, we report the large-scale emission color tunability in Ba3La(PO4)3:Tb(3+), Sm(3+) (BLPO:TS) system based on the detailed discussion on the concentration-driven selectivity of energy transfer (ET) channels from Tb(3+) to Sm(3+). It is induced by the concentration-dependent (5)D3 and (5)D4 emissions of Tb(3+) and the different interaction mechanisms of ET from Tb(3+) to Sm(3+) via (5)D3 and (5)D4 channels. In the diluted Tb(3+) scenario, the red emission of Sm(3+) is efficiently sensitized via the (5)D3 channel, while in the concentrated Tb(3+) case, the contribution of (5)D4 channel is dominant...
June 16, 2017: Inorganic Chemistry
https://www.readbyqxmd.com/read/28619638/development-and-characterization-of-cuttlefish-sepia-officinalis-skin-gelatin-protein-isolate-blend-films
#15
Hela Kchaou, Mourad Jridi, Ola Abdelhedi, Benbettaïeb Nasreddine, Thomas Karbowiak, Moncef Nasri, Frédéric Debeaufort
In the present study, cuttlefish skin gelatin (CSG) and protein isolate (CSPI) blend films were prepared and their physicochemical and antioxidant properties were characterized. CSG (4%, w/v) was blended with CSPI (3%, w/v) at different ratios and 15% of glycerol (w/w, plasticizer/polymer dry matter). Results showed that increasing of CSPI content induced lower lightness but higher redness, yellowness and color difference values compared to CSG film. In addition, CSPI film had the lowest tensile strength (TS) and elongation at break (EAB) values...
June 12, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28619535/evolution-and-regulation-of-bigelowiella-natans-light-harvesting-antenna-system
#16
Jonathan A D Neilson, Pattarasiri Rangsrikitphoti, Dion G Durnford
Bigelowiella natans is a mixotrophic flagellate and member of the chlorarachniophytes (Rhizaria), whose plastid is derived from a green algal endosymbiont. With the completion of the B. natans nuclear genome we are able to begin the analysis of the structure, function and evolution of the photosynthetic apparatus. B. natans has undergone substantial changes in photosystem structure during the evolution of the plastid from a green alga. While Photosystem II (PSII) composition is well conserved, Photosystem I (PSI) composition has undergone a dramatic reduction in accessory protein subunits...
May 31, 2017: Journal of Plant Physiology
https://www.readbyqxmd.com/read/28619534/cryptochrome-photoreceptors-in-green-algae-unexpected-versatility-of-mechanisms-and-functions
#17
Tilman Kottke, Sabine Oldemeyer, Sandra Wenzel, Yong Zou, Maria Mittag
Green algae have a highly complex and diverse set of cryptochrome photoreceptor candidates including members of the following subfamilies: plant, plant-like, animal-like, DASH and cryptochrome photolyase family 1 (CPF1). While some green algae encode most or all of them, others lack certain members. Here we present an overview about functional analyses of so far investigated cryptochrome photoreceptors from the green algae Chlamydomonas reinhardtii (plant and animal-like cryptochromes) and Ostreococcus tauri (CPF1) with regard to their biological significance and spectroscopic properties...
May 31, 2017: Journal of Plant Physiology
https://www.readbyqxmd.com/read/28619506/near-infra-red-light-attenuates-corneal-endothelial-cell-dysfunction-in-situ-and-in%C3%A2-vitro
#18
Claudia Núñez-Álvarez, Susana Del Olmo-Aguado, Jesús Merayo-Lloves, Neville N Osborne
In the present study mechanical damage to the corneal endothelium was induced by elevation of intraocular pressure (IOP, 140 mmHg, 60 min) to one eye of rats, delivered either in complete darkness or in the presence of red light (16.5 W/m(2), 3000 lx, 625-635 nm). IOP raised in the dark revealed the endothelium to be damaged as staining for the gap junction protein ZO-1 was irregular in appearance with some cells displaced in position or lost to leave gaps or holes. This damage was clearly attenuated when red light was focused through the pupil during the insult of raised IOP...
June 13, 2017: Experimental Eye Research
https://www.readbyqxmd.com/read/28619056/ophthalmic-artery-occlusion-following-neuro-embolization-of-the-external-carotid-artery-a-case-report
#19
Ng Wei Loon, Balwant Singh Gendeh, Rozman Zakaria, Jemaima Che Hamzah, Norshamsiah Md Din
BACKGROUND: Embozene® is a new neuroembolizing microsphere used to reduce intraoperative bleeding for head and neck tumours. We report a case of iatrogenic ophthalmic artery occlusion after Embozene® embolization of the external carotid artery (ECA). CASE PRESENTATION: A 22-year-old African gentleman presented with left nasal obstruction and epistaxis for 2 years and was diagnosed with nasopharyngeal carcinoma. He subsequently underwent embolization of the maxillary branch of the left ECA using Embozene® Microspheres - 250 μm in size before endoscopic tumour excision to reduce intra-operative bleeding...
June 15, 2017: BMC Ophthalmology
https://www.readbyqxmd.com/read/28618783/pulsed-light-emitting-diodes-for-higher-phytochemical-level-in-microgreens
#20
Viktorija Vaštakaitė, Akvilė Viršilė, Aušra Brazaitytė, Giedrė Samuolienė, Julė Jankauskienė, Algirdas Novičkovas, Pavelas Duchovskis
A novel research of pulsed LED lighting versus continuous lighting was conducted by analyzing phytochemicals levels in microgreens. Red pak choi (Brassica rapa var. chinensis), mustard (Brassica juncea L.) and tatsoi (Brassica rapa var. rosularis) were grown indoors under HPS lamps supplemented with monochromatic (455 nm, 470 nm, 505 nm, 590 nm and 627 nm) LEDs (total PPFD 200±10 µmol m(-2) s(-1); 16 h day(-1)). For pulsed light treatments, the frequencies at 2 Hz, 32 Hz, 256 Hz, and 1024 Hz with a duty cycle of 50% of monochromatic LEDs were applied...
June 15, 2017: Journal of Agricultural and Food Chemistry
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