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https://www.readbyqxmd.com/read/28732344/visible-light-driven-label-free-photoelectrochemical-immunosensor-based-on-tio2-s-bivo4-ag2s-nanocomposites-for-sensitive-detection-ota
#1
Jinhui Feng, Yueyun Li, Zengqiang Gao, Hui Lv, Xiaobo Zhang, Dawei Fan, Qin Wei
A label-free photoelectrochemical (PEC) platform with high visible-light activity for quantitative detection of the ochratoxin A (OTA) was developed by assembly of Ag2S nanoparticles (NPs) sensitized on titanium dioxide/red blood cell-like shape bismuth vanadate (TiO2/S-BiVO4) electrode via layer-by-layer (LBL) strategy. In this protocol, ascorbic acid was used as an efficient electron donor for scavenging photo-generated holes and inhibiting light driven electron-hole pair recombination. TiO2 has good photoelectric activity and large surface area...
July 13, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28732105/shining-a-light-on-the-arabidopsis-circadian-clock
#2
REVIEW
Rachael J Oakenfull, Seth J Davis
The circadian clock provides essential timing information to ensure optimal growth to prevailing external environmental conditions. A major time-setting mechanism (zeitgeber) in clock synchronisation is light. Differing light wavelengths, intensities and photoperiodic duration are processed for the clock-setting mechanism. Many studies on light-input pathways to the clock have focused on Arabidopsis thaliana. Photoreceptors are specific chromic proteins that detect light signals and transmit this information to the central circadian oscillator through a number of different signalling mechanisms...
July 21, 2017: Plant, Cell & Environment
https://www.readbyqxmd.com/read/28731851/the-frequency-of-occurrence-of-fish-shaped-red-blood-cells-in-different-haematologic-disorders
#3
Christoph Robier, Carolin Körber, Franz Quehenberger, Manfred Neubauer, Albert Wölfler
BACKGROUND: Red blood cells (RBC) resembling the silhouette of a fish are rarely observed in peripheral blood (PB) smears. In this study, we determined the frequency of occurrence of fish-shaped RBC in different haematologic diseases. METHODS: We examined PB smears of patients with iron deficiency anaemia (IDA) (n=23), β-thalassaemia minor (BTM) (n=30), sickle cell disease (SCD) (n=7), autoimmune haemolytic anaemia (AIHA) (n=13), microangiopathic haemolytic anaemia (MAHA) (n=11), hereditary sphaerocytosis (HS) (n=4), hereditary elliptocytosis (HE) (n=3), vitamin B12 and folate deficiency (n=15), anaemia in liver disease (LD) (n=17), myelodysplastic syndrome (MDS) (n=15), acute myeloid leukaemia (AML) (n=29), chronic myeloid leukaemia (CML) (n=18), primary myelofibrosis (PMF) (n=12), chronic myelo-monocytic leukaemia (CMML) (n=15) and 21 healthy controls by light microscopy for the occurrence of fish-shaped erythrocytes...
July 21, 2017: Clinical Chemistry and Laboratory Medicine: CCLM
https://www.readbyqxmd.com/read/28728814/a-tour-de-force-the-discovery-properties-and-function-of-piezo-channels
#4
P A Gottlieb
Mechanical transducers appear throughout cell biology and are used to convert mechanical stress into chemical or electrical signals that allow the cell to respond to environmental changes. In the past six years, a eukaryotic mechanical channel family with two members, Piezo1 and Piezo2, has been identified. Piezo1 was shown to be a cation-selective channel that does not require ancillary proteins for activity. Mouse Piezo1 is large, with over 2500 amino acids, and is not homologous to other ion channels. Both piezo channels have rapid voltage-dependent inactivation with a reversal potential near 0mV...
2017: Current Topics in Membranes
https://www.readbyqxmd.com/read/28727070/short-term-heat-stress-causes-altered-intracellular-signaling-in-oxidative-skeletal-muscle
#5
S Ganesan, C M Summers, S C Pearce, N K Gabler, R J Valentine, L H Baumgard, R P Rhoads, J T Selsby
Heat stress (HS) causes morbidities and mortalities, in part by inducing organ-specific injury and dysfunction. Further, HS markedly reduces farm animal productivity, and this is especially true for lean tissue accretion. The purpose of this investigation was to determine the extent to which short-term HS caused muscle dysfunction in skeletal muscle. We have previously found increased free radical injury in skeletal muscle following 24 h of HS. Thus, we hypothesized that HS would lead to apoptosis, autophagy, and decreased mitochondrial content in skeletal muscle...
June 2017: Journal of Animal Science
https://www.readbyqxmd.com/read/28726908/mn-4-li-co-doped-srmgal10o17-phosphor-in-glass-application-in-high-power-warm-w-leds
#6
Jiasong Zhong, Wei Xu, Qingli Chen, Shuo Yuan, Zhengguo Ji, Daqin Chen
Currently, the shortage of red components and easy aging of organic silicone still remain challenges for high-power phosphor-converted warm white light-emitting diodes (w-LEDs). Aiming to alleviate these issues, phosphor-in-glass (PiG), fabricated by co-sintering red- and yellow-emitting phosphors and low-melting glass, has been regarded as a promising color converter for w-LEDs. In this study, a Li(+),Mn(4+) co-doped SrMgAl10O17 red phosphor was synthesized via a conventional solid-state reaction. The as-prepared phosphor exhibits a deep red emission, ascribed to Mn(4+):(2)Eg → (4)A2g spin-forbidden transition in the range of 600-700 nm with a narrow-band full width at half maximum of 55 nm, and provides an ideal broadband excitation extending from 250 to 530 nm...
July 20, 2017: Dalton Transactions: An International Journal of Inorganic Chemistry
https://www.readbyqxmd.com/read/28726210/sodium-dodecyl-sulfate-cytotoxicity-towards-hacat-keratinocytes-comparative-analysis-of-methods-for-evaluation-of-cell-viability
#7
A L Rusanov, N G Luzgina, A V Lisitsa
Viability of keratinocytes of HaCaT immortalized line incubated with sodium dodecyl sulfate for 3 min, 1 and 48 h, was studied by light microscopy, MTT test, and neutral red absorption test. The IC50 values were determined for each of the studied lengths of exposure. HaCaT cells exhibited a dose-dependent decrease of viability under the effect of sodium dodecyl sulfate, proportional to the length of exposure. The values measured by different methods (MTT test and neutral red absorption test) varied, the differences were determined by the duration of exposure to sodium dodecyl sulfate...
July 18, 2017: Bulletin of Experimental Biology and Medicine
https://www.readbyqxmd.com/read/28725900/luminescence-properties-and-warm-white-led-application-of-a-ternary-alkaline-fluoride-red-phosphor-k2naalf6-mn-4-%C3%A2
#8
L Y Wang, E H Song, T T Deng, Y Y Zhou, Z F Liao, W R Zhao, B Zhou, Q Y Zhang
Herein, a Mn(4+) ion doped complex ternary-alkaline fluoride red phosphor K2NaAlF6:Mn(4+) has been synthesized through a facile two-step co-precipitation method at room temperature. The crystal structure, morphological properties and influence of the dopant concentration, temperature and humidity on luminescence properties as well as the performance of the as-synthesized phosphor used in white light emitting diodes (WLEDs) were investigated carefully. Intense absorption in the blue region (∼460 nm) and bright narrow-band red emission (∼630 nm) with high color purity were observed from this resultant powder...
July 20, 2017: Dalton Transactions: An International Journal of Inorganic Chemistry
https://www.readbyqxmd.com/read/28724968/ce-3-ion-surface-oxygen-vacancy-and-visible-light-induced-photocatalytic-dye-degradation-and-photocapacitive-performance-of-ceo2-graphene-nanostructures
#9
Mohammad Ehtisham Khan, Mohammad Mansoob Khan, Moo Hwan Cho
Cerium oxide nanoparticles (CeO2 NPs) were fabricated and grown on graphene sheets using a facile, low cost hydrothermal approach and subsequently characterized using different standard characterization techniques. X-ray photoelectron spectroscopy and electron paramagnetic resonance revealed the changes in surface states, composition, changes in Ce(4+) to Ce(3+) ratio, and other defects. Transmission electron microscopy (TEM) and high resolution TEM revealed that the fabricated CeO2 NPs to be spherical with particle size of ~10-12 nm...
July 19, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28724916/involvement-of-the-p62-nrf2-signal-transduction-pathway-on-erythrophagocytosis
#10
Inês B Santarino, Michelle S Viegas, Neuza S Domingues, Ana M Ribeiro, Miguel P Soares, Otília V Vieira
Erythrophagocytosis, the phagocytic removal of damaged red blood cells (RBC), and subsequent phagolysosome biogenesis are important processes in iron/heme metabolism and homeostasis. Phagolysosome biogenesis implies the interaction of nascent phagosomes with endocytic compartments and also autophagy effectors. Here, we report that besides recruitment of microtubule-associated protein-1-light chain 3 (LC3), additional autophagy machinery such as sequestosome 1 (p62) is also acquired by single-membrane phagosomes at very early stages of the phagocytic process and that its acquisition is very important to the outcome of the process...
July 19, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28724347/use-of-a-special-brazilian-red-light-emitting-railroad-worm-luciferase-in-bioassays-of-nek7-protein-kinase-and-creatine-kinase
#11
Arina Marina Perez, Bruno Aquino, Vadim Viviani, Jörg Kobarg
BACKGROUND: Luciferases, enzymes that catalyze bioluminescent reactions in different organisms, have been extensively used for bioanalytical purposes. The most well studied bioluminescent system is that of firefly and other beetles, which depends on a luciferase, a benzothiazolic luciferin and ATP, and it is being widely used as a bioanalytical reagent to quantify ATP. Protein kinases are proteins that modify other proteins by transferring phosphate groups from a nucleoside triphosphate, usually ATP...
July 19, 2017: BMC Biochemistry
https://www.readbyqxmd.com/read/28722049/photochrome-doped-organic-films-for-photonic-keypad-locks-and-multi-state-fluorescence
#12
Chris Ritchie, George Vamvounis, Hamid Soleimaninejad, Trevor A Smith, Evan J Bieske, Viktoras Dryza
The spectroscopic properties of poly(methyl methacrylate) polymer films doped with two kinds of photochromic molecular switches are investigated. A green-fluorescent sulfonyl diarylethene (P1) is combined with either a non-fluorescent diarylethene (P2) or red-fluorescent spiropyran (P3). Photoswitching between the colorless and colored isomers (P1: o-BTFO4 ↔ c-BTFO4, P2: o-DTE ↔ c-DTE, P3: SP ↔ MC) enables the P1 + P2 and P1 + P3 films to be cycled through three distinct states. From the initial state (o-BTFO4 + o-DTE/SP), irradiation with UV light generates the second state (c-BTFO4 + c-DTE/MC), where c-BTFO4 → c-DTE/MC energy transfer is established...
July 19, 2017: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/28721720/multicolor-tunable-luminescence-based-on-tb-3-eu-3-doping-through-a-facile-hydrothermal-route
#13
Chao Wang, Ting Zhou, Jing Jiang, Huiyuan Geng, Zhanglei Ning, Xin Lai, Jian Bi, Daojiang Gao
Ln(3+)-doped fluoride is a far efficient material for realizing multicolor emission, which plays an important part in full-color displays, biolabeling and MRI. However, studies on the multicolor tuning properties of Ln(3+)-doped fluoride are mainly concentrated on a complicated process using three or more dopants and the principle of energy transfer mechanism is still unclear. Herein, multicolor tunable emission is successfully obtained only by co-doping with Tb(3+) and Eu(3+) ions in β-NaGdF4 submicrocrystals via a facile hydrothermal route...
July 19, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28721487/influence-of-light-intensity-and-spectral-composition-of-artificial-light-at-night-on-melatonin-rhythm-and-mrna-expression-of-gonadotropins-in-roach-rutilus-rutilus
#14
Anika Brüning, Franz Hölker, Steffen Franke, Wibke Kleiner, Werner Kloas
In this study we investigated the influence of artificial light at night (ALAN) of different intensities (0, 1, 10, 100 lx) and different colours (blue, green, red) on the daily melatonin rhythm and mRNA expression of gonadotropins in roach Rutilus rutilus, a ubiquitous cyprinid, which occur in standing and moderately flowing freshwater habitats of central Europe. Melatonin concentrations were significantly lowered under nocturnal white light already at 1 lx. Low intensity blue, green and red ALAN lowered the melatonin levels significantly in comparison to a dark control...
July 18, 2017: Fish Physiology and Biochemistry
https://www.readbyqxmd.com/read/28720973/functional-and-physicochemical-characteristics-of-cookies-prepared-from-amorphophallus-paeoniifolius-flour
#15
M Suriya, Reshu Rajput, Chagam Koteswara Reddy, Sundaramoorthy Haripriya, Mudasir Bashir
This study was undertaken to prepare novelly formulated cookies from elephant foot yam flour (EFYF) with refined wheat flour (RWF) and evaluate their proximate composition, quality characteristics, texture, pasting and organoleptic properties. The formulated cookies prepared from EFYF, substituting RWF up to 70% had sensory properties similar to that of refined flour cookies (control). EFYF and RWF blend revealed reduced water and oil absorption capacity with increased peak and final viscosities when compared with RWF...
June 2017: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/28720937/quality-characteristics-of-broiler-chicken-meat-from-free-range-and-industrial-poultry-system-for-the-consumers
#16
Débora Cristina Fernandes da Silva, Alex Martins Varela de Arruda, Alex Augusto Gonçalves
ABSTRACT: The aim of this study was to determine and compare the quality parameters of broiler chicken meat from free-range and industrial poultry system. Proximate composition, color, pH, shear force, microbial quality and sensory characteristics were evaluated. Both free-range and industrial chicken meat presented PSE (pale, soft and exudative) anomaly (L* > 53). An inverse correlation between lightness, pH and shear force was observed. The free range broiler meat had higher yellow color (b* 11...
June 2017: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/28718216/one-compound-based-highly-efficient-deep-red-to-near-infrared-thermally-activated-delayed-fluorescence-organic-solid-films-and-electroluminescent-devices
#17
Yue Wang, Chenglong Li, Ruihong Duan, Baoyan Liang, Guangchao Han, Shipan Wang, Kaiqi Ye, Yu Liu, Yuanping Yi
The design and synthesis of highly efficient deep red (DR) and near-infrared (NIR) organic emitting materials with characteristic of thermally activated delayed fluorescence (TADF) still remains a great challenge. In this contribution, we developed a strategy to construct TADF organic solid films with strong DR or NIR emission feature. The triphenylamine (TPA) and quinoxaline-6,7-dicarbonitrile (QCN) were respectively employed as electron donor (D) and acceptor (A) to synthesize a TADF compound, TPA-QCN. The TPA-QCN molecule with orange-red emission in solution was employed as a dopant to prepare DR and NIR luminescent solid thin films...
July 17, 2017: Angewandte Chemie
https://www.readbyqxmd.com/read/28717570/chip-on-the-tip-compact-flexible-endoscopic-epifluorescence-video-microscope-for-in-vivo-imaging-in-medicine-and-biomedical-research
#18
Gregor Matz, Bernhard Messerschmidt, Werner Göbel, Severin Filser, Christian S Betz, Matthias Kirsch, Ortrud Uckermann, Marcel Kunze, Sven Flämig, André Ehrhardt, Klaus-Martin Irion, Mareike Haack, Mario M Dorostkar, Jochen Herms, Herbert Gross
We demonstrate a 60 mg light video-endomicroscope with a cylindrical shape of the rigid tip of only 1.6 mm diameter and 6.7 mm length. A novel implementation method of the illumination unit in the endomicroscope is presented. It allows for the illumination of the biological sample with fiber-coupled LED light at 455 nm and the imaging of the red-shifted fluorescence light above 500 nm in epi-direction. A large numerical aperture of 0.7 leads to a sub-cellular resolution and yields to high-contrast images within a field of view of 160 μm...
July 1, 2017: Biomedical Optics Express
https://www.readbyqxmd.com/read/28717563/multiphoton-in-vivo-imaging-with-a-femtosecond-semiconductor-disk-laser
#19
Fabian F Voigt, Florian Emaury, Philipp Bethge, Dominik Waldburger, Sandro M Link, Stefano Carta, Alexander van der Bourg, Fritjof Helmchen, Ursula Keller
We use an ultrafast diode-pumped semiconductor disk laser (SDL) to demonstrate several applications in multiphoton microscopy. The ultrafast SDL is based on an optically pumped Vertical External Cavity Surface Emitting Laser (VECSEL) passively mode-locked with a semiconductor saturable absorber mirror (SESAM) and generates 170-fs pulses at a center wavelength of 1027 nm with a repetition rate of 1.63 GHz. We demonstrate the suitability of this laser for structural and functional multiphoton in vivo imaging in both Drosophila larvae and mice for a variety of fluorophores (including mKate2, tdTomato, Texas Red, OGB-1, and R-CaMP1...
July 1, 2017: Biomedical Optics Express
https://www.readbyqxmd.com/read/28716739/phototoxicity-in-a-laryngeal-cancer-cell-line-enhanced-by-a-targeting-amphiphilic-chlorin-photosensitizer
#20
Milene N O Moritz, Carlos Rossa, Kleber T de Oliveira, Marciana P Uliana, Janice R Perussi
BACKGROUND: Photodynamic therapy (PDT) has been established in several countries as an alternative therapy for the treatment of various malignancies. This therapy involves the incorporation of a photosensitizer (PS) that is activated by visible light and form reactive oxygen species leading to target cell death by apoptosis or necrosis. Previously, our group has demonstrated that CHL-T (semi-synthesized from chlorophyll a and containing a linked solubilizing group TRISMA(®)) presented a pronounced potential to induce death in HeLa cell line after PDT...
July 14, 2017: Photodiagnosis and Photodynamic Therapy
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