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https://www.readbyqxmd.com/read/29150002/gene-editing-and-crispr-therapeutics-strategies-taught-by-cell-and-gene-therapy
#1
Juan C Ramirez
A few years ago, we assisted in the demonstration for the first time of the revolutionary idea of a type of adaptive-immune system in the bacteria kingdom. This system, named CRISPR, and variants engineered in the lab, have been demonstrated as functional with extremely high frequency and fidelity in almost all eukaryotic cells studied to date. The capabilities of this RNA-guided nuclease have added to the interest that was announced with the advent of previous technologies for genome editing tools, such as ZFN and TALEN...
2017: Progress in Molecular Biology and Translational Science
https://www.readbyqxmd.com/read/29148594/metabolic-study-of-paeoniflorin-and-total-paeony-glucosides-from-paeoniae-radix-rubra-in-rats-by-high-performance-liquid-chromatography-coupled-with-sequential-mass-spectrometry-uplc-esi-ms-n
#2
Lijun Zhu, Shanshan Sun, Yanxi Hu, Yufeng Liu
A clear understanding of the metabolism of Traditional Chinese Medicine (TCM) is extremely important in their rational clinical application and effective material foundation research. A novel and reliable strategy was performed to find more metabolites of paeoniflorin, determine the metabolites of total paeony glucosides (TPG) by means of determining those metabolites of paeoniflorin, and compare the metabolism differences between paeoniflorin and TPG by intragastric administration. This strategy was characterized as follows: firstly, the rats were divided into two groups (the paeoniflorin group and the TPG group) to find different metabolism mechanism between paeoniflorin and TPG; secondly, UPLC-FT-ICR MS and UPLC-Q-TOF MS(2) were applied to obtain accurate molecular weight and structural information respectively; thirdly, the metabolites were tentatively identified by a combination of data-processing methods including mass defect screening, characteristic neutral loss screening and product ion screening; finally, a comparative study was employed in the metabolism of paeoniflorin and TPG...
November 17, 2017: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/29141604/genome-wide-identification-of-mirnas-and-lncrnas-in-cajanus-cajan
#3
Chandran Nithin, Amal Thomas, Jolly Basak, Ranjit Prasad Bahadur
BACKGROUND: Non-coding RNAs (ncRNAs) are important players in the post transcriptional regulation of gene expression (PTGR). On one hand, microRNAs (miRNAs) are an abundant class of small ncRNAs (~22nt long) that negatively regulate gene expression at the levels of messenger RNAs stability and translation inhibition, on the other hand, long ncRNAs (lncRNAs) are a large and diverse class of transcribed non-protein coding RNA molecules (> 200nt) that play both up-regulatory as well as down-regulatory roles at the transcriptional level...
November 15, 2017: BMC Genomics
https://www.readbyqxmd.com/read/29140570/rapid-screening-of-the-endodermal-differentiation-potential-of-human-pluripotent-stem-cells
#4
Richard Siller, Gareth J Sullivan
Human pluripotent stem cells (hPSCs) hold tremendous promise for regenerative medicine, disease modeling, toxicology screening, and developmental biology. These applications are hindered due to inherent differences in differentiation potential observed among different hPSC lines. This is particularly true for the differentiation of hPSCs toward the endodermal lineage. Several groups have developed methods to screen hPSCs for their endodermal differentiation potential (EP). Particularly notable studies include (i) the use of WNT3A expression as a predictive biomarker, (ii) an embryoid body-based screen, and (iii) a transcriptomics-based approach...
November 15, 2017: Current Protocols in Stem Cell Biology
https://www.readbyqxmd.com/read/29127487/towards-a-unified-understanding-of-lithium-action-in-basic-biology-and-its-significance-for-applied-biology
#5
REVIEW
Eric Jakobsson, Orlando Argüello-Miranda, See-Wing Chiu, Zeeshan Fazal, James Kruczek, Santiago Nunez-Corrales, Sagar Pandit, Laura Pritchet
Lithium has literally been everywhere forever, since it is one of the three elements created in the Big Bang. Lithium concentration in rocks, soil, and fresh water is highly variable from place to place, and has varied widely in specific regions over evolutionary and geologic time. The biological effects of lithium are many and varied. Based on experiments in which animals are deprived of lithium, lithium is an essential nutrient. At the other extreme, at lithium ingestion sufficient to raise blood concentration significantly over 1 mM/, lithium is acutely toxic...
November 10, 2017: Journal of Membrane Biology
https://www.readbyqxmd.com/read/29127266/injuries-in-female-and-male-elite-taekwondo-athletes-a-10-year-prospective-epidemiological-study-of-1466-injuries-sustained-during-250-000-training-hours
#6
Ki Jun Park, Brian Byung Song
OBJECTIVES: We aimed to determine the injury patterns associated with training activities in elite South Korean taekwondo athletes training for the Olympic Games. METHODS: We collected data prospectively from 2007 to 2016 at the Korea National Training Center in Seoul, South Korea. A sports injury was defined as acute or chronic musculoskeletal signs and symptoms due to taekwondo activities during training sessions. Athletes were assessed by an on-site sports medicine specialist...
November 10, 2017: British Journal of Sports Medicine
https://www.readbyqxmd.com/read/29125753/current-advances-on-the-structure-bioactivity-synthesis-and-metabolic-regulation-of-novel-ubiquinone-derivatives-in-the-edible-and-medicinal-mushroom-antrodia-cinnamomea
#7
Bo-Bo Zhang, Peng-Fei Hu, Jing Huang, Yong-Dan Hu, Lei Chen, Gan-Rong Xu
In recent years, Antrodia cinnamomea has attracted great attention around the world as an extremely precious edible and medicinal mushroom. Ubiquinone derivatives, which are characteristic metabolites of A. cinnamomea, have shown great bioactivities. Some of them have been regarded as promising therapeutic agents and approved into clinical trial by US FDA. Although some excellent reviews have been published covering different aspects of A. cinnamomea, this review brings, for the first time, complete information about the structure, bioactivity, chemical synthesis, biosynthesis and metabolic regulation of ubiquinone derivatives in A...
November 10, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/29124375/reductive-augmentation-of-the-breast
#8
Paul E Chasan
AIM: Although breast reduction surgery plays an invaluable role in the correction of macromastia, it almost always results in a breast lacking in upper pole fullness and/or roundness. We present a technique of breast reduction combined with augmentation termed "reductive augmentation" to solve this problem. The technique is also extremely useful for correcting breast asymmetry, as well as revising significant pseudoptosis in the patient who has previously undergone breast augmentation with or without mastopexy...
November 9, 2017: Aesthetic Plastic Surgery
https://www.readbyqxmd.com/read/29121005/de-novo-variants-in-the-alternative-exon-5-of-scn8a-cause-epileptic-encephalopathy
#9
(no author information available yet)
PurposeAs part of the Epilepsy Genetics Initiative, we re-evaluated clinically generated exome sequence data from 54 epilepsy patients and their unaffected parents to identify molecular diagnoses not provided in the initial diagnostic interpretation.MethodsWe compiled and analyzed exome sequence data from 54 genetically undiagnosed trios using a validated analysis pipeline. We evaluated the significance of the genetic findings by reanalyzing sequence data generated at Ambry Genetics, and from a number of additional case and control cohorts...
October 2, 2017: Genetics in Medicine: Official Journal of the American College of Medical Genetics
https://www.readbyqxmd.com/read/29120894/advances-in-pharmacotherapies-for-movement-disorders-in-children-current-limitations-and-future-progress
#10
Jean-Pierre Lin
PURPOSE OF REVIEW: In childhood, movement disorders are generated by a very large number of disorders of the nervous system, and the very different developmental ages at which these occur make studies of pharmacotherapy efficacy extremely difficult. In most clinical practices, medication used in management is by trial and error, and limited by lack of efficacy and/or adverse drug reactions leading to drug intolerance. Nevertheless, symptom reduction using polypharmacy must be balanced against any accompanying comorbidities such as poor attention and concentration, constipation, ileus, urinary retention, blurred vision sedation and respiratory depression...
December 2017: Current Opinion in Pediatrics
https://www.readbyqxmd.com/read/29120703/maternal-obesity-gestational-weight-gain-and-asthma-in-offspring
#11
Kristen J Polinski, Jihong Liu, Nansi S Boghossian, Alexander C McLain
INTRODUCTION: Obesity is common among women of childbearing age; intrauterine exposure to maternal obesity or gestational weight gain may influence the development of asthma in early childhood. We examined the relationships of maternal obesity and gestational weight gain with asthma in offspring. METHODS: We used data from the Early Childhood Longitudinal Study-Birth Cohort, which has a nationally representative sample of children followed from birth in 2001 through age 4 (n = 6,450)...
November 9, 2017: Preventing Chronic Disease
https://www.readbyqxmd.com/read/29119957/-the-psychological-security-in-the-framework-of-the-system-of-factors-responsible-for-the-effectiveness-of-spa-and-health-resort-based-rehabilitation
#12
T M Krasnyanskaya, V G Tylets
AIM: The present work was designed to address the problem of ensuring the psychological security in the framework of organization of the rehabilitative treatment based at the spa and health resort facilities. MATERIAL AND METHODS: We undertook the questionnaire study for which 650 subjects were recruited among those attending spa and health resort facilities of the cities of Essentuki and Kislovodsk. The participants were asked to estimate the level of their personal security and the quality of the provided means for the rehabilitative treatment and general health improvement...
2017: Voprosy Kurortologii, Fizioterapii, i Lechebnoĭ Fizicheskoĭ Kultury
https://www.readbyqxmd.com/read/29119027/dosing-of-ertapenem-in-an-extreme-obesity-a-case-report-of-250%C3%A2-kg-patient
#13
Jana Lass, Kadri Tamme, Karin Kipper, Joel Starkopf
Limited available data for dosing in obesity of the medicines used in this case are discussed, with the emphasis on ertapenem. The case illustrates the difficulties in dosing medicines to morbidly overweight patients. The number of such patients is increasing but data on adequate doses of medicines are scarce. We demonstrate that ertapenem 1,5 g i.v. once daily provided adequate drug exposure for susceptible bacteria in a 250 kg patient with normal renal function. The case suggests the usefulness of therapeutic drug monitoring of antibiotics, especially in critically ill patients...
2017: Case Reports in Critical Care
https://www.readbyqxmd.com/read/29118424/methods-for-scarless-selection-free-generation-of-human-cells-and-allele-specific-functional-analysis-of-disease-associated-snps-and-variants-of-uncertain-significance
#14
Nicole B Coggins, Jacob Stultz, Henriette O'Geen, Luis G Carvajal-Carmona, David J Segal
With the continued emergence of risk loci from Genome-Wide Association studies and variants of uncertain significance identified from patient sequencing, better methods are required to translate these human genetic findings into improvements in public health. Here we combine CRISPR/Cas9 gene editing with an innovative high-throughput genotyping pipeline utilizing KASP (Kompetitive Allele-Specific PCR) genotyping technology to create scarless isogenic cell models of cancer variants in ~1 month. We successfully modeled two novel variants previously identified by our lab in the PALB2 gene in HEK239 cells, resulting in isogenic cells representing all three genotypes for both variants...
November 8, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29116983/-rising-to-the-level-of-your-incompetence-exploring-what-physicians-self-assessment-of-their-performance-reveals-about-the-impact-of-the-imposter-syndrome-in-medicine
#15
Kori A LaDonna, Shiphra Ginsburg, Christopher Watling
PURPOSE: Mistakes are ubiquitous in medicine; when confronted by error, physicians may experience anxiety, guilt, and self-doubt. Feedback may be useful for navigating these feelings, but only if it matches a physician's self-assessment; self-doubt and the imposter syndrome are examples of inaccurate self-assessments that may affect receptivity to feedback. The impact of real or imagined underperformance on seemingly competent physicians is poorly understood. This study aimed to develop a deeper understanding in order to identify strategies to support all physicians who struggle...
November 7, 2017: Academic Medicine: Journal of the Association of American Medical Colleges
https://www.readbyqxmd.com/read/29115906/challenges-of-running-a-gmp-facility-for-regenerative-medicine-in-a-public-hospital
#16
Mariele Viganò, Rosaria Giordano, Lorenza Lazzari
Advanced therapy medicinal products represent a new generation of medicinal products for regenerative medicine. Since the implementation of the EU regulation for this innovative class of drugs, the academic and hospital institutions have played a central role in their development and manufacture. For these institutions that are not familiar with the industrial context, being in compliance with the pharmaceutical standards is extremely challenging. This report describes how we dealt with some specific issues during our hospital-based GMP experience...
November 8, 2017: Regenerative Medicine
https://www.readbyqxmd.com/read/29108136/application-and-optimisation-of-the-comparison-on-extreme-laboratory-tests-cert-algorithm-for-detection-of-adverse-drug-reactions-transferability-across-national-boundaries
#17
Mun Yee Tham, Qing Ye, Pei San Ang, Liza Y Fan, Dukyong Yoon, Rae Woong Park, Zheng Jye Ling, James W Yip, Bee Choo Tai, Stephen Jw Evans, Cynthia Sung
PURPOSE: The Singapore regulatory agency for health products (Health Sciences Authority), in performing active surveillance of medicines and their potential harms, is open to new methods to achieve this goal. Laboratory tests are a potential source of data for this purpose. We have examined the performance of the Comparison on Extreme Laboratory Tests (CERT) algorithm, developed by Ajou University, Korea, as a potential tool for adverse drug reaction detection based on the electronic medical records of the Singapore health care system...
November 6, 2017: Pharmacoepidemiology and Drug Safety
https://www.readbyqxmd.com/read/29107890/potentially-inappropriate-prescribing-according-to-stopp-2-criteria-among-patients-discharged-from-internal-medicine-prevalence-involved-drugs-and-economic-cost
#18
Alfredo José Pardo-Cabello, Victoria Manzano-Gamero, Mónica Zamora-Pasadas, Francisco Gutiérrez-Cabello, David Esteva-Fernández, Juan de Dios Luna-Del Castillo, Juan Jiménez-Alonso
AIM: This study aims to determine the prevalence of potentially inappropriate prescribing (PIP) among patients discharged from Internal Medicine, the drugs and factors associated and economic cost of PIP. METHODS: This retrospective cross-sectional, single-center study included participants aged ≥65 years consecutively discharged from the Internal Medicine Unit in a tertiary hospital of Southern Spain. PIP was defined by the Screening Tool for Older Persons Prescriptions (STOPP-2) criteria version 2 (2015 update)...
November 2, 2017: Archives of Gerontology and Geriatrics
https://www.readbyqxmd.com/read/29106407/highly-accurate-fluorogenic-dna-sequencing-with-information-theory-based-error-correction
#19
Zitian Chen, Wenxiong Zhou, Shuo Qiao, Li Kang, Haifeng Duan, X Sunney Xie, Yanyi Huang
Eliminating errors in next-generation DNA sequencing has proved challenging. Here we present error-correction code (ECC) sequencing, a method to greatly improve sequencing accuracy by combining fluorogenic sequencing-by-synthesis (SBS) with an information theory-based error-correction algorithm. ECC embeds redundancy in sequencing reads by creating three orthogonal degenerate sequences, generated by alternate dual-base reactions. This is similar to encoding and decoding strategies that have proved effective in detecting and correcting errors in information communication and storage...
November 6, 2017: Nature Biotechnology
https://www.readbyqxmd.com/read/29104735/tracing-and-targeting-cancer-stem-cells-new-venture-for-personalized-molecular-cancer-therapy
#20
REVIEW
Ramin Radpour
Tumors consist of a mixture of heterogeneous cell types. Cancer stem cells (CSCs) are a minor sub-population within the bulk cancer fraction which has been found to reconstitute and propagate the disease and to be frequently resistant to chemotherapy, irradiation, cytotoxic drugs and probably also against immune attack. CSCs are considered as the seeds of tumor recurrence, driving force of tumorigenesis and metastases. This underlines the urgent need for innovative methods to identify and target CSCs. However, the role and existence of CSCs in therapy resistance and cancer recurrence remains a topic of intense debate...
October 26, 2017: World Journal of Stem Cells
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