keyword
MENU ▼
Read by QxMD icon Read
search

Labeled antibodies

keyword
https://www.readbyqxmd.com/read/28805931/mrp1-protein-expression-in-leukemic-stem-cells-as-a-negative-prognostic-marker-in-acute-myeloid-leukemia-patients
#1
Maria Paprocka, Aleksandra Bielawska-Pohl, Joanna Rossowska, Agnieszka Krawczenko, Danuta Duś, Marek Kiełbiński, Olga Haus, Maria Podolak-Dawidziak, Kazimierz Kuliczkowski
BACKGROUND: It is well established that expression of multi-drug resistance (MDR) proteins (MDR1, BCRP, MDR3, MRP1 and LRP) in leukemic blasts correlates with AML patients clinical response. Assuming that leukemic stem cells (LSC) are resistant to chemotherapy and responsible for relapse it might be clinically relevant to evaluate the expression level of MDR proteins in LSC and relate it to the clinical outcome. METHODS: Bone marrow samples from 26 patients with de novo AML were labeled with antibodies to distinguish CD34+CD38-CD123+ LSC population and with antibodies against MDR1, BCRP, MDR3, MRP1 or LRP proteins...
August 14, 2017: European Journal of Haematology
https://www.readbyqxmd.com/read/28805868/a-microfluidic-flow-cytometer-enabling-absolute-quantification-of-single-cell-intracellular-proteins
#2
Xiufeng Li, Beiyuan Fan, Shanshan Cao, Deyong Chen, Xiaoting Zhao, Dong Men, Wentao Yue, Junbo Wang, Jian Chen
Quantification of single-cell proteomics provides key insights into cellular heterogeneity while conventional flow cytometry cannot provide absolute quantification of intracellular proteins of single cells due to the lack of calibration approaches. This paper presents a constriction channel (with a cross sectional area smaller than cells) based microfluidic flow cytometer, capable of collecting copy numbers of specific intracellular proteins. In this platform, single cells stained with fluorescence labelled antibodies were forced to squeeze through the constriction channel with the fluorescence intensities quantified and since cells fully filled the constriction channel during the squeezing process, solutions with fluorescence labelled antibodies were flushed into the constriction channel to obtain calibration curves...
August 14, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/28805559/effect-of-stress-on-the-expression-of-galanin-receptors-in-rat-heart
#3
J Šípková, P Šída, N Kaspříková, I Kramáriková, S Hynie, V Klenerová
Neuropeptide galanin, galanin-like peptide and galanin receptors 1, 2 and 3 are a crucial part of the so-called galaninergic system. Our previous studies have shown the possible role of this system in mood modulation, especially regarding stress. So far, the galanin receptors have been found in different tissues including brain and heart. Our study deals with changes in expression of galanin receptor subtypes in the heart of Wistar rats exposed to three different types of stress. Galanin receptor subtypes were determined in fluorescently labelled samples using specific primary antibodies, and all sections were analysed in an immunofluorescent microscope and microphotographs...
2017: Folia Biologica (Praha)
https://www.readbyqxmd.com/read/28803963/model-analysis-of-bidirectional-interference-in-two-stage-labeled-ligand-immunoassays
#4
Eleanor R Lewin, Amitava Dasgupta, Roland Valdes, Douglas F Stickle
OBJECTIVES: Immunoassays involving sample incubation followed by a wash step prior to introduction of labeled analyte are potentially subject to both positive and negative interference (bidirectional interference) by a competing ligand. We examine this phenomenon from a theoretical standpoint using a mathematical model for sequential-step immunoassays in the presence of interferent. DESIGN & METHODS: Competitive binding to antibody between analyte and interferent was modeled for sequential-step immunoassays...
August 10, 2017: Clinical Biochemistry
https://www.readbyqxmd.com/read/28803843/high-throughput-screening-of-hybridoma-supernatants-using-multiplexed-fluorescent-cell-barcoding-on-live-cells
#5
Mei Lu, Brian M Chan, Peter W Schow, Wesley S Chang, Chadwick T King
With current available assay formats using either immobilized protein (ELISA, enzyme-linked immunosorbent assay) or immunostaining of fixed cells for primary monoclonal antibody (mAb) screening, researchers often fail to identify and characterize antibodies that recognize the native conformation of cell-surface antigens. Therefore, screening using live cells has become an integral and important step contributing to the successful identification of therapeutic antibody candidates. Thus the need for developing high-throughput screening (HTS) technologies using live cells has become a major priority for therapeutic mAb discovery and development...
August 10, 2017: Journal of Immunological Methods
https://www.readbyqxmd.com/read/28803798/radiosynthesis-and-preclinical-pet-evaluation-of-89-zr-nivolumab-bms-936558-in-healthy-non-human-primates
#6
Erin L Cole, Joonyoung Kim, David J Donnelly, R Adam Smith, Daniel Cohen, Virginie Lafont, Paul E Morin, Richard Y-C Huang, Patrick L Chow, Wendy Hayes, Samuel Bonacorsi
Cancer immunotherapy, unlike traditional cytotoxic chemotherapeutic treatments, engages the immune system to identify cancer cells and stimulate immune responses. The Programmed Death-1 (PD-1) protein is an immunoinhibitory receptor expressed by activated cytotoxic T-lymphocytes (CTL) that seek out and destroy cancer cells. Multiple cancer types express and upregulate the Programmed Death-Ligand 1 (PD-L1) and 2 (PD-L2) which bind to PD-1 as an immune escape mechanism. Nivolumab is a fully human IgG4 anti-PD-1 monoclonal antibody (mAb) approved for treatment of multiple cancer types...
August 4, 2017: Bioorganic & Medicinal Chemistry
https://www.readbyqxmd.com/read/28803008/two-step-large-volume-magnetic-separation-combined-with-pcr-assay-for-sensitive-detection-of-listeria-monocytogenes-in-pasteurized-milk
#7
Dan Luo, Xiaolin Huang, Yan Mao, Chaochao Chen, Fulai Li, Hengyi Xu, Yonghua Xiong
Immunomagnetic separation (IMS) is an effective tool for the preconcentration and purification of food-borne pathogens from complex food samples because of its high capture efficiency (CE). In conventional IMS, antibodies are usually conjugated on the surface of magnetic beads (MB); the random orientation and conformation changes of antibodies on the MB surface can decrease their bioactivity. Moreover, the Brownian motion of immobilized antibodies is weakened, thereby rendering their binding efficiency with bacteria lower than that of free antibodies...
August 9, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28802075/overcoming-severe-adverse-reactions-to-venom-immunotherapy-by-using-anti-ige-antibodies-in-combination-with-a-high-maintenance-dose
#8
Eva Stretz, Eva Maria Oppel, Räwer Helen-Caroline, René Chatelain, Sebastian Mastnik, Bernhard Przybilla, Franziska Ruëff
BACKGROUND: An omalizumab treatment and a high maintenance venom dose may both help to prevent recurrent systemic allergic reactions (SAR) to venom immunotherapy (VIT). The effectiveness of this combination therapy, however, is unclear. OBJECTIVE: We wanted to explore the possibility whether a temporary treatment with the anti-IgE antibody omalizumab, combined with a VIT using an elevated maintenance dose of >100 μg venom may establish a permanent tolerance of maintenance VIT...
August 12, 2017: Clinical and Experimental Allergy: Journal of the British Society for Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/28801845/accurate-analytical-method-for-human-plasma-glucagon-levels-using-liquid-chromatography-high-resolution-mass-spectrometry-comparison-with-commercially-available-immunoassays
#9
Atsushi Miyachi, Masaki Kobayashi, Eri Mieno, Moritaka Goto, Kenichi Furusawa, Takashi Inagaki, Tadahiro Kitamura
Accurate quantification of plasma glucagon levels in humans is necessary for understanding the physiological and pathological importance of glucagon. Although several immunoassays for glucagon are available, they provide inconsistent glucagon values owing to cross-reactivity of the antibodies with peptides other than glucagon. To overcome this limitation, we developed a novel method to measure glucagon levels by a liquid chromatography (LC)-high-resolution mass spectrometry (HRMS) assay via parallel reaction monitoring (PRM) without immunoaffinity enrichment...
August 12, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28797727/immunogenicity-and-safety-of-a-booster-dose-of-the-13-valent-pneumococcal-conjugate-vaccine-in-children-primed-with-the-10-valent-or-13-valent-pneumococcal-conjugate-vaccine-in-the-czech-republic-and-slovakia
#10
Ingrid Urbancikova, Roman Prymula, David Goldblatt, Lucy Roalfe, Karolina Prymulova, Pavel Kosina
BACKGROUND: Although both the 13-valent pneumococcal conjugate vaccine (PCV13) and the 10-valent pneumococcal non-typeable Haemophilus influenzae protein D-conjugate vaccine (PHiD-CV) are widely used, it is unclear how interchangeable they are in terms of immunogenicity. METHODS: Two phase 3, open-label, multicenter studies were conducted to assess the immunogenicity and safety of a booster dose of PCV13 in children primed with PHiD-CV or PCV13. In the Czech Republic, 12-15-month-old children received a PCV13 booster after 3-dose priming with either PHiD-CV or PCV13...
August 7, 2017: Vaccine
https://www.readbyqxmd.com/read/28796576/antibody-affinity-against-2009-a-h1n1-influenza-and-pandemrix-vaccine-nucleoproteins-differs-between-childhood-narcolepsy-patients-and-controls
#11
Alexander Lind, Eva Freyhult, Anita Ramelius, Tomas Olsson, Lisen Arnheim-Dahlström, Favelle Lamb, Mohsen Khademi, Aditya Ambati, Markus Maeurer, Izaura Lima Bomfim, Katharina Fink, Malin Fex, Carina Törn, Helena Elding Larsson, Åke Lernmark
Increased narcolepsy incidence was observed in Sweden following the 2009 influenza vaccination with Pandemrix(®). A substitution of the 2009 nucleoprotein for the 1934 variant has been implicated in narcolepsy development. The aims were to determine (a) antibody levels toward wild-type A/H1N1-2009[A/California/04/2009(H1N1)] (NP-CA2009) and Pandemrix-[A/Puerto Rico/8/1934(H1N1)] (NP-PR1934) nucleoproteins in 43 patients and 64 age-matched controls; (b) antibody affinity in reciprocal competitive assays in 11 childhood narcolepsy patients compared with 21 age-matched controls; and (c) antibody levels toward wild-type A/H1N1-2009[A/California/04/2009(H1N1)] (H1N1 NS1), not a component of the Pandemrix vaccine...
August 10, 2017: Viral Immunology
https://www.readbyqxmd.com/read/28796316/effects-of-tocilizumab-on-neutrophil-function-and-kinetics
#12
Laurence S C Lok, Neda Farahi, Jatinder K Juss, Chrystalla Loutsios, Chandra K Solanki, A Michael Peters, Francis Donaldson, Benjamin Porter-Brown, Edwin R Chilvers
BACKGROUND: Decreases in circulating neutrophils (polymorphonuclear leukocytes, PMNs) have been reported in patients treated with the anti-interleukin-6 receptor (IL-6R) antibody tocilizumab (TCZ); the mechanism for this is unclear. We hypothesize that TCZ reduces circulating neutrophils by affecting margination and / or bone marrow trafficking without affecting neutrophil function or apoptosis. MATERIALS AND METHODS: 18 healthy subjects were randomized to single intravenous dose of TCZ 8 mg/kg (n = 12) or placebo (n = 6) on day 0...
August 10, 2017: European Journal of Clinical Investigation
https://www.readbyqxmd.com/read/28794452/the-anti-influenza-m2e-antibody-response-is-promoted-by-xcr1-targeting-in-pig-skin
#13
Charlotte Deloizy, Even Fossum, Christophe Barnier-Quer, Céline Urien, Tiphany Chrun, Audrey Duval, Maelle Codjovi, Edwige Bouguyon, Pauline Maisonnasse, Pierre-Louis Hervé, Céline Barc, Olivier Boulesteix, Jérémy Pezant, Christophe Chevalier, Nicolas Collin, Marc Dalod, Bjarne Bogen, Nicolas Bertho, Isabelle Schwartz-Cornil
XCR1 is selectively expressed on a conventional dendritic cell subset, the cDC1 subset, through phylogenetically distant species. The outcome of antigen-targeting to XCR1 may therefore be similar across species, permitting the translation of results from experimental models to human and veterinary applications. Here we evaluated in pigs the immunogenicity of bivalent protein structures made of XCL1 fused to the external portion of the influenza virus M2 proton pump, which is conserved through strains and a candidate for universal influenza vaccines...
August 9, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28794344/papillary-thyroid-carcinoma-with-desmoid-type-fibromatosis-a-clinical-pathological-and-immunohistochemical-study-of-14-cases
#14
Nami Takada, Mitsuyoshi Hirokawa, Masahiro Ito, Aki Ito, Ayana Suzuki, Miyoko Higuchi, Seiji Kuma, Toshitetsu Hayashi, Masao Kishikawa, Shuichi Horikawa, Akira Miyauchi
Papillary thyroid carcinoma (PTC) with desmoid-type fibromatosis (DTF) is characterized by genetic alterations of the fibroblasts. However, PTC-DTF is extremely rare, and the reports on such cases have been sporadic. Immunohistochemical staining using the antibody for beta-catenin is useful in diagnosing the variant. This report aims to describe the clinical, pathological, and immunohistochemical findings in 14 cases of PTC-DTF and to clarify the diagnostic significance of the variant. The patients included 9 women and 5 men, with a mean age of 49...
August 10, 2017: Endocrine Journal
https://www.readbyqxmd.com/read/28794324/christmas-tree-derived-amplification-immuno-strategy-for-sensitive-visual-detection-of-vibrio-parahaemolyticus-based-on-gold-label-silver-stain-technology
#15
Xinxin Song, Yanjie Wu, Lin Wu, Yufang Hu, Wenrou Li, Zhiyong Guo, Xiurong Su, Xiaohua Jiang
A developed Christmas-tree derived immunosensor based on a gold label silver stain (GLSS) technique was fabricated for a highly sensitive analysis of Vibrio parahaemolyticu (VP). In this strategy, captured VP antibody (cAb) was immobilized on a solid substrate; then, the VPs were sequentially tagged with a signal probe by incubating the assay with a detection VP antibody (dAb) conjugated gold nanoparticles (AuNPs)-labeled graphite-like carbon nitride (g-C3N4). Finally, the attached signal probe could harvest a visible signal by the silver meal deposition, and then followed by homebrew Matlab 6...
2017: Analytical Sciences: the International Journal of the Japan Society for Analytical Chemistry
https://www.readbyqxmd.com/read/28794078/long-term-efficacy-and-safety-in-patients-with-rheumatoid-arthritis-continuing-on-sb4-or-switching-from-reference-etanercept-to-sb4
#16
Paul Emery, Jiří Vencovský, Anna Sylwestrzak, Piotr Leszczyński, Wieslawa Porawska, Barbara Stasiuk, Joanna Hilt, Zdenka Mosterova, Soo Yeon Cheong, Jeehoon Ghil
OBJECTIVES: SB4 (Benepali, Brenzys) is a biosimilar of reference etanercept (ETN). In a randomised, double-blind, 52-week study, SB4 demonstrated comparable efficacy and safety to ETN in patients with rheumatoid arthritis (RA). The open-label extension period evaluated long-term efficacy, safety and immunogenicity when continuing SB4 versus switching from ETN to SB4. METHODS: In the randomised, double-blind phase, patients received weekly subcutaneous administration of 50 mg SB4 or ETN with background methotrexate for up to 52 weeks...
August 9, 2017: Annals of the Rheumatic Diseases
https://www.readbyqxmd.com/read/28793328/liver-tissue-fragments-obtained-from-males-are-the-most-promising-source-of-human-hepatocytes-for-cell-based-therapies-flow-cytometric-analysis-of-albumin-expression
#17
Karolina Ewa Zakrzewska, Anna Samluk, Agnieszka Wencel, Krzysztof Dudek, Dorota Genowefa Pijanowska, Krzysztof Dariusz Pluta
Cell-based therapies that could provide an alternative treatment for the end-stage liver disease require an adequate source of functional hepatocytes. There is little scientific evidence for the influence of patient's age, sex, and chemotherapy on the cell isolation efficiency and metabolic activity of the harvested hepatocytes. The purpose of this study was to investigate whether hepatocytes derived from different sources display differential viability and biosynthetic capacity. Liver cells were isolated from 41 different human tissue specimens...
2017: PloS One
https://www.readbyqxmd.com/read/28792915/fast-rbc-loading-by-fluorescent-antibodies-and-nuclei-staining-dye-and-their-potential-bioanalytical-applications
#18
Mohamed K Al-Essa, Susanne Melzer, Attila Tarnok, Kamal A Hadidi, Mohammed El-Khateeb
This study was designed to load different antibodies (Abs) and a fluorescent dye onto the red blood cell (RBC) surface. We have used fluorescein isothiocyanate (FITC)-conjugate anti-human Ab, CD22-PE (B-cell marker-phycoerythrin Ab), and 4',6-diamidino-2-phenylindole (DAPI) for insertion over the RBC surface. In a first step, conjugation experiments were performed: in dimethyl sulfoxide (DMSO), RBCs were conserved and modified by succinic anhydride to create an additional -COOH group, and then activated with 3-(3-dimethylaminopropyl)carbodiimide-N-hydroxysuccinimide (EDC-NHS) in 2-(N-morpholino) ethanesulfonic acid hydrate buffer for insertion of labeled Abs or DAPI...
August 9, 2017: Zeitschrift Für Naturforschung. C, A Journal of Biosciences
https://www.readbyqxmd.com/read/28791648/assessment-of-specificity-of-an-adenovirus-targeted-to-her3-4
#19
Sheena H MacLeod, Kyle G Potts, Shyambabu Chaurasiya, Mary M Hitt
Gene therapy with viral vectors, such as adenovirus (Ad), targeted to the human epidermal growth factor receptors 3 and 4 (HER3/4) are potentially useful for cancer therapy. Testing the expression of a reporter gene from these viruses in target cells is essential to determine functionality of the targeted virus. A competition assay with a relevant ligand (heregulin, HRG) can provide convincing evidence that blocking binding to the HER3/4 receptor results in decreased reporter gene expression. Labeling individual viruses with a fluorescent molecule allows examination of the targeted virus in specific steps in the infection...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28790849/immunotargeting-relapsed-or-refractory-precursor-b-cell-acute-lymphoblastic-leukemia-role-of-blinatumomab
#20
REVIEW
Manon Queudeville, Rupert Handgretinger, Martin Ebinger
Patients with refractory or relapsed (R/R) acute lymphoblastic leukemia (ALL) have a dismal prognosis of around 5% long-term survival when treated with cytotoxic chemotherapy and allogenic stem cell transplantation. T-cell immunobased strategies open up new therapeutic perspectives. Blinatumomab is the first of a new class of antibody constructs that was labeled bispecific T-cell engager (BiTE): it consists of two single chain variable fragment connected with a flexible linker, one side binding CD3, the other CD19...
2017: OncoTargets and Therapy
keyword
keyword
114411
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"