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Min Yang, Iuri Perisse, Zhiqiang Fan, Misha Regouski, Mirella Meyer-Ficca, Irina A Polejaeva
Serial cloning by somatic cell nuclear transfer (SCNT) is a critical tool for the expansion of precious transgenic lines or resetting the lifespan of primary transgenic cells for multiple genetic modifications. We successfully produced second-generation cloned goats using donor neonatal fibroblasts from first-generation clones. However, our attempts to produce any third-generation clones failed. SCNT efficiency decreased progressively with the clonal generations. The rate of pregnancy loss was significantly greater in recloning groups (P<0...
May 17, 2018: Reproduction, Fertility, and Development
Jasmine Chong, Othman Soufan, Carin Li, Iurie Caraus, Shuzhao Li, Guillaume Bourque, David S Wishart, Jianguo Xia
We present a new update to MetaboAnalyst (version 4.0) for comprehensive metabolomic data analysis, interpretation, and integration with other omics data. Since the last major update in 2015, MetaboAnalyst has continued to evolve based on user feedback and technological advancements in the field. For this year's update, four new key features have been added to MetaboAnalyst 4.0, including: (1) real-time R command tracking and display coupled with the release of a companion MetaboAnalystR package; (2) a MS Peaks to Pathways module for prediction of pathway activity from untargeted mass spectral data using the mummichog algorithm; (3) a Biomarker Meta-analysis module for robust biomarker identification through the combination of multiple metabolomic datasets and (4) a Network Explorer module for integrative analysis of metabolomics, metagenomics, and/or transcriptomics data...
May 14, 2018: Nucleic Acids Research
Rodrigo A Mendes, Shawan K C Almeida, Iuri N Soares, Cristina A Barboza, Renato G Freitas, Alex Brown, Gabriel L C de Souza
In this work, we present a computational study on the antioxidant potential of myricetin 3,4[Formula: see text]-di-O-α-L-rhamnopyranoside (Compound M). A density functional theory (DFT) approach with the B3LYP and LC-ωPBE functionals and with both the 6-311G(d,p) and 6-311+G(d,p) basis sets was used. The focus of the investigation was on the structural and energetic parameters including both bond dissociation enthalpies (BDEs) and ionization potentials (IPs), which provide information on the potential antioxidant activity...
May 11, 2018: Journal of Molecular Modeling
Caio Vinícius de Mira-Mendes, Danilo Silva Ruas, Renan Manoel de Oliveira, Indira Maria Castro, Iuri Ribeiro Dias, Julio Ernesto Baumgarten, Flora Acuña Juncá, Mirco Solé
An inventory of the amphibians of the Reserva Ecológica Michelin - REM in southern Bahia, Brazil is presented. Sixty-nine species were recorded during a ten-year sampling period. Amphibians were distributed in two orders (Gymnophiona and Anura), belonging to twelve families [Aromobatidae (1), Bufonidae (3), Centrolenidae (1), Craugastoridae (5), Eleutherodactylidae (3), Hemiphractidae (2), Hylidae (34), Phyllomedusidae (5) Leptodactylidae (7), Microhylidae (4), Odontophrynidae (3) and Caeciliidae (1)]. Fifty per cent of the reproductive modes known for Atlantic forest anurans were recorded...
2018: ZooKeys
Joao Paulo Leonardo-Pinto, Cristina Laguna Benetti-Pinto, Iuri Quagliato, Daniela Angerame Yela
Thoracic endometriosis syndrome is a rare condition that includes four entities: catamenial pneumothorax, catamenial hemothorax, catamenial hemoptysis and lung nodules. We describe the case of a 23-year-old woman with complaints of hemoptysis during menstrual period in the two years prior to the appointment. Initially, a treatment for tuberculosis was established with no success. Further investigation showed a 4 mm nodule in the right lung, and the transvaginal ultrasonography indicated the presence of deep endometriosis...
April 27, 2018: Revista Brasileira de Ginecologia e Obstetrícia
Gabriela Tonini Peterle, Lucas Lima Maia, Leonardo Oliveira Trivilin, Mayara Mota de Oliveira, Joaquim Gasparini Dos Santos, Suzanny Oliveira Mendes, Elaine Stur, Lidiane Pignaton Agostini, Lília Alves Rocha, Raquel Ajub Moysés, Patrícia Maluf Cury, Fábio Daumas Nunes, Iúri Drumond Louro, Marcelo Dos Santos, Adriana Madeira Álvares da Silva
BACKGROUND: In oral squamous cell carcinoma (OSCC), the HIF-1 complex promotes the expression of genes involved in specific mechanisms of cell survival under hypoxic conditions, such as plasminogen activator inhibitor-1 (PAI-1), carbonic anhydrase 9 (CAIX) and vascular endothelial growth factor A (VEGFA). The study aimed to investigate the presence and prognostic value of PAI-1, CAIX, and VEGFA in OSCC. MATERIALS AND METHODS: Immunohistochemistry was used to analyze the expressions of these proteins in 52 tumoral tissue samples of patients with OSCC, surgically treated and followed by a minimum of 24 months after surgery...
April 25, 2018: Journal of Oral Pathology & Medicine
João Gustavo Rocha Peixoto Dos Santos, Ana Luiza Costa Zaninotto, Renato Amaro Zângaro, Ana Maria Costa Carneiro, Iuri Santana Neville, Almir Ferreira de Andrade, Manoel Jacobsen Teixeira, Wellingson Silva Paiva
BACKGROUND: Photobiomodulation describes the use of red or near-infrared light to stimulate or regenerate tissue. It was discovered that near-infrared wavelengths (800-900 nm) and red (600 nm) light-emitting diodes (LED) are able to penetrate through the scalp and skull and have the potential to improve the subnormal cellular activity of compromised brain tissue. Different experimental and clinical studies were performed to test LED therapy for traumatic brain injury (TBI) with promising results...
April 24, 2018: Trials
Victor G D Orrico, Iuri R Dias, Euvaldo Marciano-Jr
A new species of the genus Phyllodytes is described from the State of Bahia, in the Atlantic Rain Forest of Northeastern Brazil. Phyllodytes praeceptor sp. nov. can be differentiated from other species of Phyllodytes by its medium size (SVL 20.7-25.8 mm in males); odontoids moderately developed; vocal sac externally visible; eyes large and prominent; dorsum homogenously cream, except for a few scattered spots and blotches; venter areolate with two parallel, paramedial lines of larger tubercles; few tubercles in the ventral surface of thighs, the largest being the medial one; a large tubercle on the skin around the tibio-tarsal articulation; nuptial pad rounded and moderately expanded...
April 9, 2018: Zootaxa
Iury Oliveira Castro, Bruno Frazão Gribel, Ana Helena Gonçalves de Alencar, José Valladares-Neto, Carlos Estrela
PURPOSE: To compare crown inclination and angulation results obtained after orthodontic treatment to the Roth prescription. METHODS: The study design was based on files and documents obtained from a database of 26 patients who had undergone orthodontic treatment using the straight-wire technique and the Roth prescription. The crown inclination and angulation were measured using a three-dimensional (3D) cephalometric module (VistaDent, Dentsply, New York, NY, USA) by an orthodontist...
April 11, 2018: Journal of Orofacial Orthopedics, Fortschritte der Kieferorthopädie
Cintya Yukie Hayashi, Iuri Santana Neville, Priscila Aparecida Rodrigues, Ricardo Galhardoni, André Russowsky Brunoni, Ana Luiza Zaninotto, Vinicius Monteiro de Paula Guirado, Ana Sofia Cueva, Daniel Ciampi de Andrade, Manoel Jacobsen Teixeira, Wellingson Silva Paiva
Background: Overactivation of NMDA-mediated excitatory processes and excess of GABA-mediated inhibition are attributed to the acute and subacute phases, respectively, after a traumatic brain injury (TBI). However, there are few studies regarding the circuitry during the chronic phase of brain injury. Objective: To evaluate the cortical excitability (CE) during the chronic phase of TBI in victims diagnosed with diffuse axonal injury (DAI). Methods: The 22 adult subjects were evaluated after a minimum of 1 year from the onset of moderate or severe TBI...
2018: Frontiers in Neurology
Iuri Marques, Sarah Caroline Willis, Ellen Ingrid Schafheutle, Karen Hassell
Purpose Organisational culture (OC) shapes individuals' perceptions and experiences of work. However, no instrument capable of measuring specific aspects of OC in community pharmacy exists. The purpose of this paper is to report the development and validation of an instrument to measure OC in community pharmacy in Great Britain (GB), and conduct a preliminary analysis of data collected using it. Design/methodology/approach Instrument development comprised three stages: Stage I: 12 qualitative interviews and relevant literature informed instrument design; Stage II: 30 cognitive interviews assessed content validity; and Stage III: a cross-sectional survey mailed to 1,000 community pharmacists in GB, with factor analysis for instrument validation...
April 9, 2018: Journal of Health Organization and Management
Lucas de Lima Maia, Gabriela Tonini Peterle, Marcelo Dos Santos, Leonardo Oliveira Trivilin, Suzanny Oliveira Mendes, Mayara Mota de Oliveira, Joaquim Gasparini Dos Santos, Elaine Stur, Lidiane Pignaton Agostini, Cinthia Vidal Monteiro da Silva Couto, Juliana Dalbó, Aricia Leone Evangelista Monteiro de Assis, Anderson Barros Archanjo, Ana Maria Da Cunha Mercante, Rossana Veronica Mendoza Lopez, Fábio Daumas Nunes, Marcos Brasilino de Carvalho, Eloiza Helena Tajara, Iúri Drumond Louro, Adriana Madeira Álvares-da-Silva
AIMS: Jumonji Domain-Containing 1A (JMJD1A) protein promotes demethylation of histones, especially at lysin-9 of di-methylated histone H3 (H3K9me2) or mono-methylated (H3K9me1). Increased levels of H3 histone methylation at lysin-9 (H3K9) is related to tumor suppressor gene silencing. JMJD1A gene target Adrenomeduline (ADM) has shown to promote cell growth and tumorigenesis. JMJD1A and ADM expression, as well as H3K9 methylation level have been related with development risk and prognosis of several tumor types...
2018: PloS One
Priscila Marinho de Abreu, Anna Clara Gregório Có, Pedro Leite Azevedo, Isabella Bittencourt do Valle, Karine Gadioli de Oliveira, Sônia Alves Gouvea, Melissa Freitas Cordeiro-Silva, Iúri Drummond Louro, José Roberto Vasconcelos de Podestá, Jeferson Lenzi, Agenor Sena, Elismauro Francisco Mendonça, Sandra Lúcia Ventorin von Zeidler
BACKGROUND: The prevalence of high-risk human papillomavirus (HPV) DNA in cases of oral cavity squamous cell carcinoma (SCC) varies widely. The aim of this study is to investigate the frequency of high-risk HPV DNA in a large Brazilian cohort of patients with oral cavity SCC. METHODS: Biopsy and resected frozen and formalin-fixed paraffin-embedded specimens of oral cavity SCC were available from 101 patients who were recruited at two Brazilian centres. Stringent measures with respect to case selection and prevention of sample contamination were adopted to ensure reliability of the data...
March 27, 2018: BMC Cancer
Rodolfo Jaffé, Xavier Prous, Allan Calux, Markus Gastauer, Gilberto Nicacio, Robson Zampaulo, Pedro W M Souza-Filho, Guilherme Oliveira, Iuri V Brandi, José O Siqueira
The degradation of subterranean habitats is believed to represent a serious threat for the conservation of obligate subterranean dwellers (troglobites), many of which are short-range endemics. However, while the factors influencing cave biodiversity remain largely unknown, the influence of the surrounding landscape and patterns of subterranean connectivity of terrestrial troglobitic communities have never been systematically assessed. Using spatial statistics to analyze the most comprehensive speleological database yet available for tropical caves, we first assess the influence of iron cave characteristics and the surrounding landscape on troglobitic communities from the Eastern Amazon...
2018: PeerJ
Alyne Marem, Debora N Okamoto, Lilian C G Oliveira, Diego M Ruiz, Roberto A Paggi, Marcia Y Kondo, Iuri E Gouvea, Maria A Juliano, Rosana E de Castro, Luiz Juliano, Marcelo Y Icimoto
Nep (Natrialba magadii extracellular protease) is a halolysin-like peptidase secreted by the haloalkaliphilic archaeon Natrialba magadii. Many extracellular proteases have been characterized from archaea to bacteria as adapted to hypersaline environments retaining function and stability until 4.0M NaCl. As observed in other secreted halolysins, this stability can be related to the presence of a C-terminal extension (CTE) sequence. In the present work, we compared the biochemical properties of recombinant Nep protease with the truncated form at the 134 amino acids CTE (Nep∆CTE), that was more active in 4M NaCl than the non-truncated wild type enzyme...
July 1, 2018: International Journal of Biological Macromolecules
Beatriz Grinsztejn, Brenda Hoagland, Ronaldo I Moreira, Esper G Kallas, Jose V Madruga, Silvia Goulart, Iuri C Leite, Lucilene Freitas, Luana M S Martins, Thiago S Torres, Ricardo Vasconcelos, Raquel B De Boni, Peter L Anderson, Albert Liu, Paula M Luz, Valdiléa G Veloso
BACKGROUND: PrEP Brasil was a demonstration study to assess feasibility of daily oral tenofovir diphosphate disoproxil fumarate plus emtricitabine provided at no cost to men who have sex with men (MSM) and transgender women at high risk for HIV within the Brazilian public health system. We report week 48 pre-exposure prophylaxis (PrEP) retention, engagement, and adherence, trends in sexual behaviour, and incidence of HIV and sexually transmitted infections in this study cohort. METHODS: PrEP Brasil was a 48 week, open-label, demonstration study that assessed PrEP delivery at three referral centres for HIV prevention and care in Rio de Janeiro, Brazil (Fundação Oswaldo Cruz), and São Paulo, Brazil (Universidade de São Paulo and Centro de Referência e Treinamento em DST e AIDS)...
March 2018: Lancet HIV
Carlos D S Silva, Iury A Paz, Felipe D Abreu, Aurideia P de Sousa, Carla P Veríssimo, Nilberto R F Nascimento, Tércio F Paulo, Davila Zampieri, Marcos N Eberlin, Ana C S Gondim, Loraine C Andrade, Idalina M M Carvalho, Eduardo H S Sousa, Luiz G F Lopes
Nitric oxide has been involved in many key biological processes such as vasodilation, platelet aggregation, apoptosis, memory function, and this has drawn attention to the development of exogenous NO donors. Metallonitrosyl complexes are an important class of these compounds. Here, two new ruthenium nitrosyl complexes containing a thiocarbonyl ligand, with the formula cis-[Ru(phen)2 (L)(NO)](PF6 )3 (phen = phenantroline, L = thiourea or thiobenzamide), were synthesized and characterized by electronic spectroscopy, FTIR, NMR, mass spectrometry and voltammetric techniques...
May 2018: Journal of Inorganic Biochemistry
Gianpiero Fasola, Jessica Menis, Alessandro Follador, Elisa De Carlo, Francesca Valent, Giuseppe Aresu, Alessandro De Pellegrin, Francesco Giacomuzzi, David Iuri, Emilio Lugatti, Giuseppe Parisi, Guglielmo Pacileo
BACKGROUND: Non small cell lung cancer (NSCLC) diagnosis and treatment is a highly complex process, requiring managerial skills merged with clinical knowledge and experience. Integrated care pathways (ICPs) might be a good strategy to overview and improve patient's management. The aim of this study was to review the ICPs of NSCLC patients in a University Hospital and to identify areas of quality improvement. MATERIALS AND METHODS: The electronic medical records of 169 NSCLC patients visited at the University Hospital were retrospectively reviewed...
January 2018: International Journal of Technology Assessment in Health Care
Gustavo Gameiro, Pedro Monsalve, Iuri Golubev, Lori Ventura, Vittorio Porciatti
PURPOSE: The Water Drinking Test (WDT) is currently used to temporarily elevate intraocular pressure (IOP) and predict peak IOP in glaucoma. This study investigates neurovascular changes associated with WDT in normal subjects. METHODS: The effect of WDT (1 liter in 5▒min) on IOP, mean brachial blood pressure (MAP), heart rate (HR), and pattern electroretinogram (PERG) was assessed within the next hour in 16 healthy subjects (mean age 33.5±7.9▒y). As a control, testing was repeated in the same subjects without WDT one week later...
February 2, 2018: Journal of Glaucoma
Sergio L Gianizella, Thiago F Martins, Valeria C Onofrio, Nair O Aguiar, Waleska Gravena, Carlos A R do Nascimento, Laérzio C Neto, Diogo L Faria, Natália A S Lima, Monica R Solorio, Louise Maranhão, Ivan J Lima, Iury V D Cobra, Tamily Santos, Gerson P Lopes, Emiliano E Ramalho, Hermes R Luz, Marcelo B Labruna
The tick fauna of Brazil is currently composed by 72 species. The state of Amazonas is the largest of Brazil, with an area of ≈ 19% of the Brazilian land. Besides its vast geographic area, only 19 tick species have been reported for Amazonas. Herein, lots containing ticks from the state of Amazonas were examined in three major tick collections from Brazil. A total of 5933 tick specimens were examined and recorded, comprising 2693 males, 1247 females, 1509 nymphs, and 484 larvae. These ticks were identified into the following 22 species: Amblyomma cajennense sensu lato, Amblyomma calcaratum, Amblyomma coelebs, Amblyomma dissimile, Amblyomma dubitatum, Amblyomma geayi, Amblyomma goeldii, Amblyomma humerale, Amblyomma latepunctatun, Amblyomma longirostre, Amblyomma naponense, Amblyomma oblongoguttatum, Amblyomma ovale, Amblyomma rotundatum, Amblyomma scalpturatum, Amblyomma varium, Dermacentor nitens, Haemaphysalis juxtakochi, Ixodes cf...
February 2018: Experimental & Applied Acarology
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