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https://www.readbyqxmd.com/read/28889055/cells-qpcr-as-a-direct-quantitative-pcr-method-to-avoid-microbial-dna-extractions-in-grape-musts-and-wines
#1
Verónica Soares-Santos, Isabel Pardo, Sergi Ferrer
A novel quantitative PCR assay called Cells-qPCR has been developed for the rapid detection and quantification of yeasts, lactic acid bacteria (LAB) and acetic acid bacteria (AAB) directly from grape must and wine that does not require DNA extraction. The assay was tested on Brettanomyces bruxellensis, Saccharomyces cerevisiae, Lactobacillus plantarum, Oenococcus oeni, Acetobacter aceti and Gluconobacter oxydans in culture media, and in white and red grape musts and wines. Standard curves were constructed from DNA and cells for the six target species in all the matrices...
September 4, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/28863341/acetic-acid-bacteria-in-fermented-foods-and-beverages
#2
REVIEW
Jonas De Roos, Luc De Vuyst
Although acetic acid bacteria (AAB) are commonly found in spontaneous or backslopped fermented foods and beverages, rather limited knowledge about their occurrence and functional role in natural food fermentation ecosystems is available. Not only is their cultivation, isolation, and identification difficult, their cells are often present in a viable but not culturable state. Yet, they are promising starter cultures either to better control known food fermentation processes or to produce novel fermented foods and beverages...
August 29, 2017: Current Opinion in Biotechnology
https://www.readbyqxmd.com/read/28822049/potential-for-diagnosis-versus-therapy-monitoring-of-attention-deficit-hyperactivity-disorder-a-new-epigenetic-biomarker-interacting-with-both-genotype-and-auto-immunity
#3
Walter Adriani, Emilia Romano, Mariangela Pucci, Esterina Pascale, Luca Cerniglia, Silvia Cimino, Renata Tambelli, Paolo Curatolo, Oleg Granstrem, Mauro Maccarrone, Giovanni Laviola, Claudio D'Addario
In view of the need for easily accessible biomarkers, we evaluated in ADHD children the epigenetic status of the 5'-untranslated region (UTR) in the SLC6A3 gene, coding for human dopamine transporter (DAT). We analysed buccal swabs and sera from 30 children who met DSM-IV-TR criteria for ADHD, assigned to treatment according to severity. Methylation levels at six-selected CpG sites (among which, a CGGCGGCGG and a CGCG motif), alone or in combination with serum titers in auto-antibodies against dopamine transporter (DAT aAbs), were analysed for correlation with CGAS scores (by clinicians) and Conners' scales (by parents), collected at recruitment and after 6 weeks...
August 18, 2017: European Child & Adolescent Psychiatry
https://www.readbyqxmd.com/read/28800944/systematic-review-tumor-associated-antigen-autoantibodies-and-ovarian-cancer-early-detection
#4
REVIEW
Renée Turzanski Fortner, Antje Damms-Machado, Rudolf Kaaks
OBJECTIVES: Tumor-associated autoantibodies (AAbs), produced as an immune response to tumor-associated antigens (TAAs), are a novel pathway of early detection markers. METHODS: We conducted a systematic review on AAbs and ovarian cancer to summarize the diagnostic performance of individual AAbs and AAb panels. A total of 29 studies including 85 AAbs were included; 27 of the studies were conducted in prevalent cases and cancer-free controls and 2 investigations included pre-diagnosis samples...
August 8, 2017: Gynecologic Oncology
https://www.readbyqxmd.com/read/28724905/flowering-time-in-banana-musa-spp-a-day-neutral-plant-is-controlled-by-at-least-three-flowering-locus-t-homologues
#5
Akhilesh K Chaurasia, Hemant B Patil, Bal Krishna, V R Subramaniam, Prafullachandra V Sane, Aniruddha P Sane
Banana is an important day neutral food crop with a long flowering/fruiting cycle that is affected by hot summers or cold winters in different places. Manipulating its life cycle requires an understanding of its flowering time machinery to bypass these stresses. Twelve FLOWERING LOCUS T (FT) and two TWIN SISTER OF FT (TSF) members were isolated from banana and their organization and expression pattern studied during development in two varieties that differ in flowering time namely Grand Nain (AAA genotype) and Hill banana (AAB genotype)...
July 19, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28679329/autoantibodies-against-%C3%AE-1-adrenergic-receptor-response-to-induction-therapy-with-bortezomib-containing-regimens-for-multiple-myeloma-patients
#6
Wen Gao, Wen-Jia Guo, Dong-Yan Hou, Guang-Zhong Yang, Yin Wu, Yan-Chen Li, Yun Leng, Yu Tang, Lin Xu, Jia-Mei Liu, Hua Wang, Xin Wang, Juan Zhang, Wen-Shu Zhao, Wen-Ming Chen, Lin Zhang
This study aims to investigate the predictive value of pre-chemotherapy β1R-AABs by evaluating the response of newly diagnosed symptomatic multiple myeloma (MM) patients to their treatment with a bortezomib-containing regimen. Forty-five de novo MM patients and 50 normal controls (NCs) were prospectively enrolled in this study. Serum titers of β1R-AABs were detected by ELISA. These 45 MM patients were divided into two groups (positive and negative groups) according to their β1R-AABs. Follow-up examinations were performed on these patients during chemotherapy induction...
July 6, 2017: Leukemia & Lymphoma
https://www.readbyqxmd.com/read/28667320/complete-genome-sequence-of-the-cellulose-producing-strain-komagataeibacter-nataicola-rzs01
#7
Heng Zhang, Xuran Xu, Xiao Chen, Fanshu Yuan, Bianjing Sun, Yunhua Xu, Jiazhi Yang, Dongping Sun
Komagataeibacter nataicola is an acetic acid bacterium (AAB) that can produce abundant bacterial cellulose and tolerate high concentrations of acetic acid. To globally understand its fermentation characteristics, we present a high-quality complete genome sequence of K. nataicola RZS01. The genome consists of a 3,485,191-bp chromosome and 6 plasmids, which encode 3,514 proteins and bear three cellulose synthase operons. Phylogenetic analysis at the genome level provides convincing evidence of the evolutionary position of K...
June 30, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28665954/prevalence-and-correlation-of-cytokine-specific-autoantibodies-with-epidemiological-factors-and-c-reactive-protein-in-8-972-healthy-individuals-results-from-the-danish-blood-donor-study
#8
Jakob Hjorth von Stemann, Andreas Stribolt Rigas, Lise Wegner Thørner, Daniel Guldager Kring Rasmussen, Ole Birger Pedersen, Klaus Rostgaard, Christian Erikstrup, Henrik Ullum, Morten Bagge Hansen
Natural cytokine-specific autoantibodies (c-aAb) have been measured in healthy and diseased individuals, and have been considered as both endogenous immune-regulators and pathogenic factors. Overall, the etiology and potential pathology of c-aAb are still undefined. To further characterize the sero-prevalence, predictors and consequences of high c-aAb levels, we performed the largest population-based study of c-aAb to date, using participants and epidemiological data from the Danish Blood Donor Study. Using a validated bead-based multiplex assay we assessed plasma levels of IL-1α, IL-6, IL-10, IFNα and GM-CSF-specific c-aAb in 8,972 healthy blood donors...
2017: PloS One
https://www.readbyqxmd.com/read/28631819/protective-effects-of-tannic-acid-on-pressure-overload-induced-cardiac-hypertrophy-and-underlying-mechanisms-in-rats
#9
Li Chu, Pinya Li, Tao Song, Xue Han, Xuan Zhang, Qiongtao Song, Tao Liu, Yuanyuan Zhang, Jianping Zhang
OBJECTIVES: The aim of this study was to examine the cardioprotective effects and latent mechanism of tannic acid (TA) on cardiac hypertrophy. METHODS: Abdominal aortic banding (AAB) was used to induce pressure overload-induced cardiac hypertrophy in male Wistar rats, sham-operated rats served as controls. AAB rats were treated with TA (20 and 40 mg/kg) or captoril. KEY FINDINGS: Abdominal aortic banding rats that received TA showed ameliorated pathological changes in cardiac morphology and coefficients, decreased cardiac hypertrophy and apoptosis, a reduction in over expressions of angiotensin type 1 receptor (AT1 R), angiotensin type 2 receptor (AT2 R), phosphorylation of extracellular signal-regulated kinase 1/2 (ERK1/2) and transforming growth factor-β (TGF-β) mRNA, and modified expression of matrix metal proteinase-9 (MMP-9) mRNA in AAB rat hearts...
June 20, 2017: Journal of Pharmacy and Pharmacology
https://www.readbyqxmd.com/read/28619110/effect-of-aspartic-acid-and-glutamate-on-metabolism-and-acid-stress-resistance-of-acetobacter-pasteurianus
#10
Haisong Yin, Renkuan Zhang, Menglei Xia, Xiaolei Bai, Jun Mou, Yu Zheng, Min Wang
BACKGROUND: Acetic acid bacteria (AAB) are widely applied in food, bioengineering and medicine fields. However, the acid stress at low pH conditions limits acetic acid fermentation efficiency and high concentration of vinegar production with AAB. Therefore, how to enhance resistance ability of the AAB remains as the major challenge. Amino acids play an important role in cell growth and cell survival under severe environment. However, until now the effects of amino acids on acetic fermentation and acid stress resistance of AAB have not been fully studied...
June 15, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28615718/genome-wide-analyses-of-sweet-family-proteins-reveal-involvement-in-fruit-development-and-abiotic-biotic-stress-responses-in-banana
#11
Hongxia Miao, Peiguang Sun, Qing Liu, Yulu Miao, Juhua Liu, Kaixing Zhang, Wei Hu, Jianbin Zhang, Jingyi Wang, Zhuo Wang, Caihong Jia, Biyu Xu, Zhiqiang Jin
Sugars Will Eventually be Exported Transporters (SWEET) are a novel type of sugar transporter that plays crucial roles in multiple biological processes. From banana, for the first time, 25 SWEET genes which could be classified into four subfamilies were identified. Majority of MaSWEETs in each subfamily shared similar gene structures and conserved motifs. Comprehensive transcriptomic analysis of two banana genotypes revealed differential expression patterns of MaSWEETs in different tissues, at various stages of fruit development and ripening, and in response to abiotic and biotic stresses...
June 14, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28596995/cardiac-myosin-binding-protein-c-autoantibodies-are-potential-early-indicators-of-cardiac-dysfunction-and-patient-outcome-in-acute-coronary-syndrome
#12
Thomas L Lynch, Diederik W D Kuster, Beverly Gonzalez, Neelam Balasubramanian, Nandini Nair, Sharlene Day, Jenna E Calvino, Yanli Tan, Christoph Liebetrau, Christian Troidl, Christian W Hamm, Ahmet Güçlü, Barbara McDonough, Ali J Marian, Jolanda van der Velden, Christine E Seidman, Gordon S Huggins, Sakthivel Sadayappan
The degradation and release of cardiac myosin binding protein-C (cMyBP-C) upon cardiac damage may stimulate an inflammatory response and autoantibody (AAb) production. We determined whether the presence of cMyBP-C-AAbs associated with adverse cardiac function in CVD patients. Importantly, cMyBP-C-AAbs were significantly detected in ACS patient sera upon arrival to the emergency department, particularly in STEMI patients. Patients positive for cMyBP-C-AAbs had a reduced LVEF and elevated levels of clinical biomarkers of MI...
April 2017: JACC. Basic to Translational Science
https://www.readbyqxmd.com/read/28595995/mir-146b-probably-assists-mirna-146a-in%C3%A2-the-suppression-of-keratinocyte-proliferation-and-inflammatory-responses%C3%A2-in-psoriasis
#13
Helen Hermann, Toomas Runnel, Alar Aab, Hansjörg Baurecht, Elke Rodriguez, Nathaniel Magilnick, Egon Urgard, Liisi Šahmatova, Ele Prans, Julia Maslovskaja, Kristi Abram, Maire Karelson, Bret Kaldvee, Paula Reemann, Uku Haljasorg, Beate Rückert, Paulina Wawrzyniak, Michael Weichenthal, Ulrich Mrowietz, Andre Franke, Christian Gieger, Jonathan Barker, Richard Trembath, Lam C Tsoi, James T Elder, Eric R Tkaczyk, Kai Kisand, Pärt Peterson, Külli Kingo, Mark Boldin, Stephan Weidinger, Cezmi A Akdis, Ana Rebane
miR-146a inhibits inflammatory responses in human keratinocytes and in different mouse models of skin inflammation. Little is known about the role of miR-146b in the skin. In this study, we confirmed the increased expression of miR-146a and miR-146b (miR-146a/b) in the lesional skin of patients with psoriasis. The expression of miR-146a was approximately twofold higher than that of miR-146b in healthy human skin, and it was more strongly induced by stimulation of proinflammatory cytokines in keratinocytes and fibroblasts...
September 2017: Journal of Investigative Dermatology
https://www.readbyqxmd.com/read/28576368/efficacy-of-gaseous-ozone-to-counteract-postharvest-table-grape-sour-rot
#14
L Pinto, L Caputo, L Quintieri, S de Candia, F Baruzzi
This work aims at studying the efficacy of low doses of gaseous ozone in postharvest control of the table grape sour rot, a disease generally attributed to a consortium of non-Saccharomyces yeasts (NSY) and acetic acid bacteria (AAB). Sour rot incidence of wounded berries, inoculated with 8 NSYstrains, or 7 AAB, or 56 yeast-bacterium associations, was monitored at 25 °C up to six days. Sour rot incidence in wounded berries inoculated with yeast-bacterium associations resulted higher than in berries inoculated with one single NSY or AAB strain...
September 2017: Food Microbiology
https://www.readbyqxmd.com/read/28557506/correction-to-chun-et-al-2017
#15
(no author information available yet)
Reports an error in "A psychometric investigation of gender differences and common processes across borderline and antisocial personality disorders" by Seokjoon Chun, Alexa Harris, Margely Carrion, Elizabeth Rojas, Stephen Stark, Carl Lejuez, William V. Lechner and Marina A. Bornovalova (Journal of Abnormal Psychology, 2017[Jan], Vol 126[1], 76-88). In the original record, there were two errors in the article's suppemental material. The supplemental material stated, "In each case, if the relaxed model fit significantly better than the baseline model (i...
May 29, 2017: Journal of Abnormal Psychology
https://www.readbyqxmd.com/read/28537647/robotic-assisted-total-hip-arthroplasty-outcomes-at-minimum-two-year-follow-up
#16
Richard L Illgen, Brandon R Bukowski, Rasheed Abiola, Paul Anderson, Morad Chughtai, Anton Khlopas, Michael A Mont
BACKGROUND: Component malposition in total hip arthroplasty (THA) contributes to instability and early failure. Robotic-assisted total hip arthroplasty (rTHA) utilizes CT-based planning with haptically-guided bone preparation and implant insertion to optimize component position accuracy. This study compared acetabular component position and postoperative complications following manual THA (mTHA) with rTHA. MATERIALS AND METHODS: Consecutive primary THAs performed by one surgeon at three intervals were analyzed in this retrospective cohort study: the initial 100 consecutive manual THAs (mTHA) in clinical practice (year 2000), the last consecutive 100 mTHA before rTHA introduction (year 2011), and the first consecutive 100 rTHA (year 2012)...
May 24, 2017: Surgical Technology International
https://www.readbyqxmd.com/read/28526919/antibodies-to-post-translationally-modified-insulin-as-a-novel-biomarker-for-prediction-of-type-1-diabetes-in-children
#17
Rocky Strollo, Chiara Vinci, Nicola Napoli, Paolo Pozzilli, Johnny Ludvigsson, Ahuva Nissim
AIMS/HYPOTHESIS: We have shown that autoimmunity to insulin in type 1 diabetes may result from neoepitopes induced by oxidative post-translational modifications (oxPTM). Antibodies specific to oxPTM-insulin (oxPTM-INS-Ab) are present in most newly diagnosed individuals with type 1 diabetes and are more common than autoantibodies to native insulin. In this study, we investigated whether oxPTM-INS-Ab are present before clinical onset of type 1 diabetes, and evaluated the ability of oxPTM-INS-Ab to identify children progressing to type 1 diabetes...
August 2017: Diabetologia
https://www.readbyqxmd.com/read/28503176/impact-of-autoantibodies-against-glycolytic-enzymes-on-pathogenicity-of-autoimmune-retinopathy-and-other-autoimmune-disorders
#18
REVIEW
Grazyna Adamus
Autoantibodies (AAbs) against glycolytic enzymes: aldolase, α-enolase, glyceraldehyde-3-phosphate dehydrogenase, and pyruvate kinase are prevalent in sera of patients with blinding retinal diseases, such as paraneoplastic [cancer-associated retinopathy (CAR)] and non-paraneoplastic autoimmune retinopathies, as well as in many other autoimmune diseases. CAR is a degenerative disease of the retina characterized by sudden vision loss in patients with cancer and serum anti-retinal AAbs. In this review, we discuss the widespread serum presence of anti-glycolytic enzyme AAbs and their significance in autoimmune diseases...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28432850/a-detailed-experimental-study-of-a-dna-computer-with-two-endonucleases
#19
Sebastian Sakowski, Tadeusz Krasiński, Joanna Sarnik, Janusz Blasiak, Jacek Waldmajer, Tomasz Poplawski
Great advances in biotechnology have allowed the construction of a computer from DNA. One of the proposed solutions is a biomolecular finite automaton, a simple two-state DNA computer without memory, which was presented by Ehud Shapiro's group at the Weizmann Institute of Science. The main problem with this computer, in which biomolecules carry out logical operations, is its complexity - increasing the number of states of biomolecular automata. In this study, we constructed (in laboratory conditions) a six-state DNA computer that uses two endonucleases (e...
July 14, 2017: Zeitschrift Für Naturforschung. C, A Journal of Biosciences
https://www.readbyqxmd.com/read/28428295/multiple-genome-sequences-of-heteropolysaccharide-forming-acetic-acid-bacteria
#20
Julia U Brandt, Frank Jakob, Andreas J Geissler, Jürgen Behr, Rudi F Vogel
We report here the complete genome sequences of the acetic acid bacteria (AAB) Acetobacter aceti TMW 2.1153, A. persici TMW 2.1084, and Neoasaia chiangmaiensis NBRC 101099, which secrete biotechnologically relevant heteropolysaccharides (HePSs) into their environments. Upon genome sequencing of these AAB strains, the corresponding HePS biosynthesis pathways were identified.
April 20, 2017: Genome Announcements
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