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Cordyceps militaris

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https://www.readbyqxmd.com/read/28197639/cordycepin-induces-apoptosis-in-sgc%C3%A2-7901-cells-through-mitochondrial-extrinsic-phosphorylation-of-pi3k-akt-by-generating-ros
#1
Moussa Ide Nasser, Muqaddas Masood, Wei Wei, Xiaochun Li, Yifa Zhou, Bao Liu, Jiang Li, Xiaomeng Li
Medicinal plants are affluent sources of several effectual natural drugs. Among them cordycepin which is extracted from Cordyceps militaris is a hopeful chemotherapy agent due to its extensive anti-inflammatory, anti-proliferative, antioxidant, and antitumor characteristics. This study investigated the efficacy of cordycepin in the context of human gastric cancer SGC‑7901 and searched for the cell death procedure. Cordycepin incorporates mitochondrial-mediated apoptosis in SGC‑7901 cells with the help of regulating mitochondrial extrinsic pathways by inhibition of A3AR and drive activation of DR3, which promote the activation of PI3K/Akt protein expression as well as collapse of mitochondrial membrane potential (MMP)...
January 25, 2017: International Journal of Oncology
https://www.readbyqxmd.com/read/28074332/functional-analysis-of-ribonucleotide-reductase-from-cordyceps-militaris-expressed-in-escherichia-coli
#2
Tatsuya Kato, Suparmin Ahmad, Enoch Y Park
Cordyceps militaris produces cordycepin (3'-deoxyadenosine), which has various activities, including anti-oxidant, anti-tumoral, anti-viral, and anti-inflammatory. Ribonucleotide reductase (RNR) seems to be a candidate to produce cordycepin in C. militaris because RNR catalyzes the reduction of nucleotides to 2'-deoxynucleotides, whose structures are similar to that of cordycepin. However, the role of RNR has not been confirmed yet. In this study, complementary DNAs (cDNAs) of C. militaris RNR (CmRNR) large and small subunits (CmR1 and CmR2) were cloned from C...
January 10, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/28071691/comparative-mitochondrial-genomics-toward-exploring-molecular-markers-in-the-medicinal-fungus-cordyceps-militaris
#3
Shu Zhang, Ai-Jing Hao, Yu-Xiang Zhao, Xiao-Yu Zhang, Yong-Jie Zhang
Cordyceps militaris is a fungus used for developing health food, but knowledge about its intraspecific differentiation is limited due to lack of efficient markers. Herein, we assembled the mitochondrial genomes of eight C. militaris strains and performed a comparative mitochondrial genomic analysis together with three previously reported mitochondrial genomes of the fungus. Sizes of the 11 mitochondrial genomes varied from 26.5 to 33.9 kb mainly due to variable intron contents (from two to eight introns per strain)...
January 10, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28068557/cordycepin-inhibits-migration-of-human-glioblastoma-cells-by-affecting-lysosomal-degradation-and-protein-phosphatase-activation
#4
Dueng-Yuan Hueng, Ching-Hsuan Hsieh, Yu-Chen Cheng, Wen-Chiuan Tsai, Ying Chen
Cordycepin, a nucleoside-derivative-isolated form Cordyceps militaris, has been reported to suppress tumor cell proliferation and cause apoptosis. This study investigates the effect of cordycepin on the migration of human glioblastoma cells. Cordycepin suppressed the migration of the human glioblastoma cell lines U87MG and LN229 in transwell and wound healing assays. Cordycepin decreased protein expression of integrin α1, focal adhesion kinase (FAK), p-FAK, paxillin and p-paxillin. The lysosomal inhibitor NH4Cl blocked the ability of cordycepin to inhibit focal adhesion protein expression and glioma cell migration...
December 29, 2016: Journal of Nutritional Biochemistry
https://www.readbyqxmd.com/read/28035061/cordycepin-induces-autophagy-mediated-c-flipl-degradation-and-leads-to-apoptosis-in-human-non-small-cell-lung-cancer-cells
#5
Xinghui Yu, Jianya Ling, Xianfang Liu, Sen Guo, Yidan Lin, Xiangguo Liu, Ling Su
Cordycepin, a main active composition extracted from Cordyceps militaris, has been reported to exert anti-tumor activity in a broad spectrum of cancer types. However, the function of cordycepin on human non-small cell lung cancer cells is still obscure. Our present work showed that cordycepin inhibited cell growth by inducing apoptosis and autophagy in human NSCLC cells. Further study revealed that cordycepin triggered extrinsic apoptosis associated with down-regulation of c-FLIPL which suppresses the activity of caspase-8...
January 24, 2017: Oncotarget
https://www.readbyqxmd.com/read/28008812/anti-inflammation-properties-of-fruiting-bodies-and-submerged-cultured-mycelia-of-culinary-medicinal-higher-basidiomycetes-mushrooms
#6
Rao-Chi Chien, Lan-Min Lin, Yuan-Hua Chang, Yu-Chieh Lin, Pei-Hsuan Wu, Mikheil D Asatiani, Sophie-Gabrielle Wasser, Maxim Krakhmalnyi, Abed Agbarya, Solomon P Wasser, Jeng-Leun Mau
This research shows the phenolic composition and anti-inflammation properties of fruiting bodies and mycelia of 15 strains of 12 species of higher Basidiomycetes medicinal mushrooms. In this research, 15 extracts were prepared and their effects on inflammation-related mediators in RAW 264.7 cells were evaluated. In the extracts, amounts of total phenols ranged from 8.47 to 70.32 gallic acid equivalents mg/g and amounts of flavonoids ranged from 0.13 to 15.21 rutin equivalents mg/g. The production of nitric oxide, tumor necrosis factor-α, and interleukin-6 was decreased at different levels by these extracts, whereas the production of interleukin-10 was increased by 6 of the extracts...
2016: International Journal of Medicinal Mushrooms
https://www.readbyqxmd.com/read/27987993/structural-elucidation-and-immunostimulatory-activity-of-polysaccharide-isolated-by-subcritical-water-extraction-from-cordyceps-militaris
#7
Xiaoping Luo, Yuqing Duan, Wenya Yang, Haihui Zhang, Changzheng Li, Jixian Zhang
Water-soluble polysaccharides were obtained from Cordyceps militaris (C. militaris) (CMP) by subcritical water extraction (SWE). Two polysaccharides fractions, CMP-W1 and CMP-S1, were isolated from CMP using DEAE-52 cellulose and Sephadex G-150 column chromatography. The structural characteristics of CMP-W1 and CMP-S1 were investigated. The results showed that the molecular weight of CMP-W1 and CMP-S1 are 3.66×105Da and 4.60×105Da, respectively, and both of them were heteropolysaccharides composed of d-mannose, d-glucose, d-galactose with the molar ratios of 2...
February 10, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/27927214/antiplatelet-and-antithrombotic-effects-of-cordycepin-enriched-wib-801ce-from-cordyceps-militaris-ex-vivo-in-vivo-and-in-vitro
#8
Hyuk-Woo Kwon, Jung-Hae Shin, Deok Hwi Lim, Woo Jeong Ok, Gi Suk Nam, Min Ji Kim, Ho-Kyun Kwon, Jun-Hee Noh, Je-Young Lee, Hyun-Hong Kim, Jong-Lae Kim, Hwa-Jin Park
BACKGROUND: A species of the fungal genus Cordyceps has been used as a complementary and alternative medicine of traditional Chinese medicine, and its major component cordycepin and cordycepin-enriched WIB-801CE are known to have antiplatelet effects in vitro. However, it is unknown whether they have also endogenous antiplatelet and antithrombotic effects. In this study, to resolve these doubts, we prepared cordycepin-enriched WIB-801CE, an ethanol extract from Cordyceps militaris-hypha, then evaluated its ex vivo, in vivo, and in vitro antiplatelet and antithrombotic effects...
December 7, 2016: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/27910792/optimization-of-liquid-fermentation-conditions-and-protein-nutrition-evaluation-of-mycelium-from-the-caterpillar-medicinal-mushroom-cordyceps-militaris-ascomycetes
#9
Jie Gang, Han Liu, Yanhong Liu
Cordyceps militaris is a well-known traditional Chinese medicinal mushroom. In this study, the mycelium of C. militaris was cultured using liquid fermentation technology and the culture medium components were optimized by the orthogonal test method. Our results showed that the optimal medium combination for the mycelium growth is 3% glucose, 3% peptone, 0.1% MgSO4, and 0.2% KH2PO4 The international general nutritional assessment method was applied to the overall evaluation of the protein nutrition value of submerged cultivated mycelium and fruit body of C...
2016: International Journal of Medicinal Mushrooms
https://www.readbyqxmd.com/read/27832180/cordyceps-militaris-treatment-preserves-renal-function-in-type-2-diabetic-nephropathy-mice
#10
Sung-Hsun Yu, Navneet Kumar Dubey, Wei-Shan Li, Ming-Che Liu, Han-Sun Chiang, Sy-Jye Leu, Ying-Hua Shieh, Feng-Chou Tsai, Win-Ping Deng
Diabetic nephropathy is derived from long-term effects of high blood glucose on kidney function in type 2 diabetic patients. Several antidiabetic drugs and herbal medications have failed to prevent episodes of DN. Hence, this study aimed to further investigate the renal injury-reducing effect of antidiabetic CmNo1, a novel combination of powders of fruiting bodies and mycelia of Cordyceps militaris. After being administered with streptozotocin-nicotinamide and high-fat-diet, the diabetic nephropathy mouse model displayed elevated blood glucose and renal dysfunction markers including serum creatinine and kidney-to-body weight ratio...
2016: PloS One
https://www.readbyqxmd.com/read/27747890/evaluation-of-different-agricultural-wastes-for-the-production-of-fruiting-bodies-and-bioactive-compounds-by-medicinal-mushroom-cordyceps-militaris
#11
Qunying Lin, Liangkun Long, Liangliang Wu, Fenglun Zhang, Shuling Wu, Weiming Zhang, Xiaoming Sun
BACKGROUND: In commercial production of Cordyceps militaris (a famous Chinese medicine), usually cereal grains were utilized as cultivation substrates. This study is aimed to evaluate the efficiency of agricultural wastes as substitute materials in the low cost production of C. militaris. Cottonseed shells (CS), corn cob particles (CCP), Italian poplar sawdusts (IPS) and substrates spent by Flammulina velutipes (SS) were employed to cultivate C. militaris, using rice medium as control...
October 17, 2016: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/27717908/actions-of-water-extract-from-cordyceps-militaris-in-hyperuricemic-mice-induced-by-potassium-oxonate-combined-with-hypoxanthine
#12
Tianqiao Yong, Minglong Zhang, Diling Chen, Ou Shuai, Shaodan Chen, Jiyan Su, Chunwei Jiao, Delong Feng, Yizhen Xie
ETHNOPHARMACOLOGICAL RELEVANCE: Cordyceps militaris was recorded in the classic traditional Chinese medicine book with the main functions of "protecting liver and enhancing kidney functions", influencing serum uric acid levels. AIM OF STUDY: The aim is to investigate the hypouricemic effects and possible mechanism of C. militaris in hyperuricemic mice. MATERIALS AND METHODS: A water extract (WECM) was prepared by decocting C. militaris directly at 80 °C in water bath, followed by lyophilization...
December 24, 2016: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/27689974/cordycepin-induces-apoptosis-and-inhibits-proliferation-of-human-lung-cancer-cell-line-h1975-via-inhibiting-the-phosphorylation-of-egfr
#13
Zheng Wang, Xue Wu, Yan-Ni Liang, Li Wang, Zhong-Xing Song, Jian-Li Liu, Zhi-Shu Tang
Cordycepin is an active component of the traditional Chinese medicine Cordyceps sinensis and Cordyceps militaris with notable anticancer activity. Though the prominent inhibitory activity was reported in different kinds of cancer cell lines, the concrete mechanisms remain elusive. It was reported that cordycepin could be converted into tri-phosphates in vivo to confuse a number of enzymes and interfere the normal cell function. For the inhibitory mechanism of EGFR inhibitors and the structure similarity of ATP and tri-phosphated cordycepin, human lung cancer cell line H1975 was employed to investigate the inhibitory effect of cordycepin...
2016: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/27667538/synthesis-characterization-and-antioxidant-activity-of-selenium-polysaccharide-from-cordyceps-militaris
#14
Zhen-Yuan Zhu, Fei Liu, Hui Gao, Huiqing Sun, Meng Meng, Yong-Min Zhang
A purified selenium-containing derivatives of Cordyceps militaris polysaccharide synthesized using H2SeO3/HNO3 and BaCl2 as a catalyst was investigated in this paper. The reaction condition was optimized by selecting different reaction temperature and period. Afterward, the one with the highest Se content was purified by ultra-filtration device with a molecular cut off size of 10KDa. Followed by its structural characterizations. Results of IFR and (13)C NMR spectroscopy indicated that C-6 substitution was predominant in selenized polysaccharide...
December 2016: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/27649728/effects-of-illumination-pattern-during-cultivation-of-fruiting-body-and-bioactive-compound-production-by-the-caterpillar-medicinal-mushroom-cordyceps-militaris-ascomycetes
#15
Chiu-Yeh Wu, Zeng-Chin Liang, Chin-Yin Tseng, Shu-Hui Hu
We investigated the effects of light intensity in the 3 cultivation stages separately-the mycelium colonization stage, the primordial initiation stage, and the fruiting stage (in order)-on fruiting body and bioactive compound production by Cordyceps militaris. In the mycelium colonization stage, rice substrates were incubated in a spawn running room at 23°C. During the primordial initiation stage, C. militaris was grown at 18°C and illuminated 12 hours/day. In the fruiting stage the temperature was 23°C, with illumination provided 12 hours/day...
2016: International Journal of Medicinal Mushrooms
https://www.readbyqxmd.com/read/27642126/biochemical-characterization-of-a-novel-fibrinolytic-enzyme-from-cordyceps-militaris
#16
Xiaolan Liu, Narasimha-Kumar Kopparapu, Yao Li, Yongping Deng, Xiqun Zheng
A fibrinolytic enzyme was produced by the medicinal mushroom, Cordyceps militaris using submerged fermentation. The enzyme was purified from culture supernatant by hydrophobic interaction, ion exchange and gel filtration chromatographies. It was purified by 36 fold, with a specific activity of 1,467.4U/mg protein and the final yield was 5.8%. The molecular weight of the enzyme as determined by SDS-PAGE and gel filtration was 28kDa and 24.5kDa, respectively, and its isoelectric point (pI) was 9.0±0.2. It was found to be a glycoprotein with carbohydrate content of 1...
January 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/27588098/separation-of-cordycepin-from-cordyceps-militaris-fermentation-supernatant-using-preparative-hplc-and-evaluation-of-its-antibacterial-activity-as-an-nad-dependent-dna-ligase-inhibitor
#17
Xiaofeng Zhou, Guoqiang Cai, Yi He, Guotong Tong
Cordycepin exhibits various bio-activities, including anticancer, antibacterial, antiviral and immune regulation activities, and is a significant focus of research. However, the preparation of high-purity cordycepin remains challenging. Also, the molecular target with which cordycepin interacts to cause an antibacterial effect remains unknown. In the present study, cordycepin was prepared by preparative high-performance liquid chromatography (prep-HPLC) and the purity obtained was 99.6%, indicating that this technique may be useful for the large-scale isolation of cordycepin in the future...
September 2016: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/27567378/preparative-isolation-of-cordycepin-n-6-2-hydroxyethyl-adenosine-and-adenosine-from-cordyceps-militaris-by-macroporous-resin-and-purification-by-recycling-high-speed-counter-current-chromatography
#18
Zhong Zhang, Tuernisan Tudi, Yanfang Liu, Shuai Zhou, Na Feng, Yan Yang, Chuanhong Tang, Qingjiu Tang, Jingsong Zhang
In this study, cordycepin, N(6)-(2-hydroxyethyl)-adenosine (HEA) and adenosine from the fruiting bodies of Cordyceps militaris were separated by using macroporous resin NKA-II adsorption. The parameters of static adsorption were tested and the optimized conditions were as follow: the total adsorption time was 12h, 50% ethanol was used for desorption and the desorption time was 9h. The crude sample that was prepared by macroporous resin NKA-II contained 3.4% cordycepin, 3.7% HEA and 4.9% adenosine. Then the crude sample was further purified by recycling high-speed counter-current chromatography (HSCCC) with ethyl acetate, n-butanol, 1...
October 15, 2016: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/27565291/preparation-and-inhibition-on-%C3%AE-d-glucosidase-of-low-molecular-weight-polysaccharide-from-cordyceps-militaris
#19
Zhen-Yuan Zhu, Ming-Zhu Guo, Fei Liu, You Luo, Lu Chen, Meng Meng, Xiao-Ting Wang, Yong-Min Zhang
The structural properties and the inhibition on α-d-glucosidase activity of the low molecular weight (LCMPs-II) obtained from the optimized acid hydrolysis of the Cordyceps militaris polysaccharides (CMPs) were investigated in this paper. The LCMPs-II with a molecular weight of 28 KDa mainly composed of rhamnose, xylose and glucose with the molar ratio of 1: 2.19: 6.73 was separated from LCMPs-I which was the acid hydrolysis product of CMPs by chromatography on Sephadex G-100 column. The solubility of LCMPs-II was tested to be 32...
December 2016: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/27481299/comparison-of-major-bioactive-compounds-of-the-caterpillar-medicinal-mushroom-cordyceps-militaris-ascomycetes-fruiting-bodies-cultured-on-wheat-substrate-and-pupae
#20
Mingmin Guo, Suping Guo, Yang Huaijun, Ning Bu, Cai-Hong Dong
In this study, the main bioactive compounds of the fruit bodies of Cordyceps militaris-such as adenosine, cordycepin, polysaccharides, mannitol, superoxide dismutase (SOD), and carotenoids-were cultivated on wheat and pupae, as well as sclerotium (the pupae portion) and sclerotium with fruiting bodies. The amounts of adenosine and polysaccharide in all the tested samples (except for the polysaccharides of sclerotium) are higher than the quality standards (adenosine ≥0.055% and polysaccharide ≥2.5%) determined by the Ministry of Health of the People's Republic of China...
2016: International Journal of Medicinal Mushrooms
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