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Cordyceps militaris

Nachon Raethong, Kobkul Laoteng, Wanwipa Vongsangnak
The cellular metabolic adaptations of Cordyceps militaris have been progressively studied. In particular, the cordycepin pathway is of interest in medicinal applications. Even though the metabolic pathways for cordycepin production are known to be related to different carbon sources, the regulatory mechanisms at a systems level are poorly characterized. To explore the regulatory mechanisms, this study therefore aimed to investigate the global metabolic response to cordycepin production in C. militaris through transcriptome analysis and genome-scale network-driven analysis...
June 18, 2018: Scientific Reports
Purushotham Reddy Sudina, Damoder Reddy Motati, Aravind Seema
Nonenolide (1) was first isolated from the entomopathogenic fungus Cordyceps militaries BCC2816 and exhibited good antimalarial activity against Plasmodium falciparum K1. Structurally, it features a decanolide with a trans-double bond attached to two chiral hydroxy groups, making the total synthesis of the exclusive isomer of 1 more difficult. Herein, we report the successful synthesis of 1 by employing a MacMillan α-hydroxylation to generate three chiral centers in both the key fragments, starting from 1,6-hexanediol and 1,4-butanediol, followed by Steglich esterification of compounds 2 and 3...
June 11, 2018: Journal of Natural Products
Liang Ma, Min Zhang, Shuyong Zhao
BACKGROUND: Cordyceps militaris is a medicine and food dual-purpose mushroom extensively cultivated and consumed in East and Southeast Asia for centuries. However, it has an extremely short shelf life of 3-4 days at room temperature. Cordyceps militaris was pasteurized for 10, 20 and 30 min by radio frequency (RF) at the electrode gap of 20 mm. The effect of radio frequency heating on the microbial load, color, texture and flavor attributes of Cordyceps militaris was evaluated and compared with those sterilized by conventional high pressure steam (HPS)...
May 24, 2018: Journal of the Science of Food and Agriculture
Lin Chen, Yuetao Liu, Qingfeng Guo, Qingxia Zheng, Wancun Zhang
In this study, a systematic research on the metabolome differences between wild Ophiocordyceps sinensis (O. sinensis) and artificial cultured Cordyceps militaris (C. militaris) was conducted using liquid chromatography-mass spectrometry. Principal components analysis (PCA) and Orthogonal projection on latent structure-discriminant analysis (OPLS-DA) results showed that C. militaris grown on solid rice medium (R-CM) and C. militaris grown on tussah pupa (T-CM) evidently separated and individually separated from wild O...
May 11, 2018: Biomedical Chromatography: BMC
Guangxin Yuan, Liping An, Yunpeng Sun, Guangyu Xu, Peige Du
Our previous research revealed that Cordyceps militaris can improve the learning and memory, and although the main active ingredient should be its polypeptide complexes, the underlying mechanism of its activity remains poorly understood. In this study, we explored the mechanisms by which Cordyceps militaris improves learning and memory in a mouse model. Mice were given scopolamine hydrobromide intraperitoneally to establish a mouse model of learning and memory impairment. The effects of Cordyceps polypeptide in this model were tested using the Morris water maze test; serum superoxide dismutase activity; serum malondialdehyde levels; activities of acetyl cholinesterase, Na+-k+-ATPase, and nitric oxide synthase; and gamma aminobutyric acid and glutamate contents in brain tissue...
2018: Evidence-based Complementary and Alternative Medicine: ECAM
Ning-Ning Yang, Ning Jiang, Qing-Yun Ma, Fan-Dong Kong, Qing-Yi Xie, Li-Man Zhou, Zhi-Fang Yu, You-Xing Zhao
Chemical investigation on the cultures of the fungus Cordyceps spp., a strain from cell fusion between Cordyceps militaris and Cordyceps cicadae, resulted in the isolation of 13 compounds including 2 new ones named 2-(5-(3-oxobutyl) furan-2-yl) acetic acid (1) and cordycepone (2). Their structures were elucidated from the analysis of 1D/2D NMR and CD data. Among them, compounds 1, 7-9, at a concentration of 50 μg/ml, showed weak inhibitory activity against AChE. Moreover, compounds 6, 9, and 11 showed moderate inhibitory activity against the nematode Panagrellus redivivus with mortality ratio of 79...
April 19, 2018: Journal of Asian Natural Products Research
Yu Xiao, Mingmei Sun, Qiuqin Zhang, Yulian Chen, Junqing Miao, Xin Rui, Mingsheng Dong
The effects of solid-state fermentation with Cordyceps militaris (L.) Fr. on the nutritional, physicochemical, and functional properties as well as angiotensin I converting enzyme (ACE) inhibitory activity of red bean ( Phaseolus angularis [Willd.] W.F. Wight.) flour were determined. Fermentation increased the amount of small peptides but significantly decreased large peptides. Fermentation also increased proteins and essential amino acids (by 9.31 and 13.89%, respectively) and improved the in vitro protein digestibility (6...
April 2018: Journal of Food Science and Technology
HaiWei Lou, ZhiWei Ye, Fan Yun, JunFang Lin, LiQiong Guo, BaiXiong Chen, ZhiXian Mu
The macrofungus Cordyceps militaris contains many kinds of bioactive ingredients that are regulated by functional genes, but the functions of many genes in C. militaris are still unknown. In this study, to improve the frequency of homologous integration, a genetic transformation system based on a split-marker approach was developed for the first time in C. militaris to knock out a gene encoding a terpenoid synthase (Tns). The linear and split-marker deletion cassettes were constructed and introduced into C...
May 2018: Molecular Biotechnology
Zhang Jiaojiao, Wang Fen, Liu Kuanbo, Liu Qing, Yang Ying, Dong Caihong
Cordyceps militaris is a highly valued edible and medicinal fungus due to its production of various metabolites, including adenosine, cordycepin, N6 -(2-hydroxyethyl)-adenosine, and carotenoids. The contents of these metabolites are indicative of the quality of commercially available fruit body of this fungus. In this work, the effects of environmental abiotic factors, including heat and light stresses, on the fruit body growth and metabolite production in C. militaris were evaluated during the late growth stage...
May 2018: Applied Microbiology and Biotechnology
Wangshi Xie, Zhang Zhang, Liyan Song, Chunhua Huang, Zhongyi Guo, Xianjing Hu, Sixue Bi, Rongmin Yu
Background: Cordyceps militaris fraction (CMF) has been shown to possess in vitro antitumor activity against human chronic myeloid leukemia K562 cells in our previous research. Materials and Methods: The in vitro inhibitory activities of CMF on the growth of KB cells were evaluated by viability assay. The apoptotic and cell cycle influences of CMF were detected by 4',6-diamidino-2-phenylindole staining and flow cytometry assay. The expression of different apoptosis-associated proteins and cell cycle regulatory proteins was examined by Western blot assay...
January 2018: Pharmacognosy Magazine
Shih-Jeng Huang, Fu-Kuei Huang, Yu-Shan Li, Shu-Yao Tsai
Cordyceps militaris , a medicinal and edible mushroom, was used to ferment buckwheat and embryo rice by solid-state fermentation (SSF). Our aim was to investigate the effect of ultraviolet B (UVB) light irradiation on the content of vitamin D2 and biologically effective components, and antioxidant properties of buckwheat and embryo rice in SSF with C. militaris . Irradiated samples of buckwheat and embryo rice fermented by C. militaris had significantly increased vitamin D2 content, from 0-0.3 to 1.18-16.79 μg/g, while the increase in fresh embryo rice fermented by C...
December 2017: Food Technology and Biotechnology
Xiao-Fei Wu, Min Zhang, Bhesh Bhandari, Zhongqin Li
BACKGROUND: Microwave-assisted pulse-spouted bed freeze-drying (MPSFD) uses microwave heating to substitute the customary conduction heating during freeze drying (FD), resulting in high-quality dried samples with a short drying time. The aim of this study was to determine the effect of MPSFD on volatile compounds and structural aspects of Cordyceps militaris. In order to highlight the effect of MPSFD, FD (recognized for its high-quality products) and hot-air dying (HD; the most commonly used) were also evaluated using headspace solid-phase micro-extraction combined with gas chromatography-mass spectrometry and mid-Fourier transform infrared spectroscopy...
March 8, 2018: Journal of the Science of Food and Agriculture
Ha-Kyoung Kwon, Woo-Ri Jo, Hye-Jin Park
BACKGROUND: Cordyceps militaris (C. militaris) is reported to exert various immune-activities. To enhance its activity, we fermented C.militaris with Pediococcus pentosaceus ON89A (GRC-ON89A). In this study, we investigated the immune-enhancing activity GRC-ON89A, using immunosuppressed model. METHODS: Immunosuppression was induced by intraperitoneal injection of cyclophosphamide (CY). Each group was orally administered distilled water, GRC-ON89A or GRC, respectively...
February 23, 2018: BMC Complementary and Alternative Medicine
Shuang Yang, Mingzhu Zheng, Yong Cao, Yanjiao Dong, Sanabil, Jingsheng Liu
Cordyceps militaris 202 is a potential fungus for biotransformation zein, due to its various proteases, high tolerance and viability in nature. In this article, single factor experiment and response surface methodology were applied to optimize the liquid fermentation conditions and improve the ability of biotransformation zein. The optimized fermentation conditions were as follows: inoculum concentration of 19%, volume of liquor of 130mL/500mL and pH of 4.7. Under this condition, the degree of hydrolysis (DH) was 27...
February 13, 2018: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
Tianqiao Yong, Shaodan Chen, Yizhen Xie, Diling Chen, Jiyan Su, Ou Shuai, Chunwei Jiao, Dan Zuo
Recently, we've reported the anti-hyperuricemic effects of Cordyceps militaris . As a characteristic compound of C. militaris , we hypothesized that cordycepin may play a role in preventing hyperurecimia. Remarkably, cordycepin produced important anti-hyperuricemic actions, decreasing SUA (serum uric acid) to 216, 210, and 203 μmol/L ( P < 0.01) at 15, 30, and 60 mg/kg in comparison of hyperuricemic control (337 μmol/L), closing to normal control (202 μmol/L). Elisa, RT-PCR and western blot analysis demonstrated that the actions may be attributed to its downregulation of uric acid transporter 1 (URAT1) in kidney...
2018: Frontiers in Microbiology
Pin Chanjula, Anusorn Cherdthong
The objective of this experiment was to study the effects of dried spent mushroom Cordyceps militaris (SMCM) supplementation on digestibility, rumen fermentation, and blood metabolite parameters in goats. When the mushroom production and harvesting was complete, SMCM was collected fresh from a mushroom farm. Four 18-mo-old male crossbred (Thai Native × Anglo Nubian) goats with initial BW of 26.0 ± 1.40 kg were randomly assigned according to a 4 × 4 Latin square design to receive four different levels of SMCM at 0, 100, 200, and 300 g/d...
April 3, 2018: Journal of Animal Science
Min Zhang, Yuanlong Shan, Hongtao Gao, Bin Wang, Xin Liu, Yuanyuan Dong, Xiuming Liu, Na Yao, Yonggang Zhou, Xiaowei Li, Haiyan Li
BACKGROUND: Antibiotic residues can cause antibiotic resistance in livestock and their food safety-related issues have increased the consumer demand for products lacking these residues. Hence, developing safe and effective antibiotic alternatives is important to the animal feed industry. With their strong antibacterial actions, antimicrobial peptides have potential as antibiotic alternatives. RESULTS: We investigated the antibacterial and immunomodulatory activities and the mechanisms of action of an antimicrobial peptide...
February 5, 2018: Microbial Cell Factories
Ke-Chun Bai, Fuu Sheu
Cordyceps militaris is a dietary therapeutic fungus that is an important model species in Cordyceps research. In this study, we purified a novel protein from the fruit bodies of C. militaris and designated it as Cordyceps militaris protein (CMP). CMP has a molecular mass of 18.0 kDa and is not glycosylated. Interestingly, CMP inhibited cell viability in murine primary cells and other cell lines in a time- and dose-dependent manner. Using trypan blue staining and a lactate dehydrogenase release assay, we showed that CMP caused cell death in the murine hepatoma cell line BNL 1MEA...
January 2018: Journal of Food and Drug Analysis
Liang-Tzung Lin, Ying-Jang Lai, She-Ching Wu, Wei-Hsuan Hsu, Chen-Jei Tai
Cordycepin is one of the most crucial bioactive compounds produced by Cordyceps militaris and has exhibited antitumor activity in various cancers. However, industrial production of large amounts of cordycepin is difficult. The porcine liver is abundant in proteins, vitamins, and adenosine, and these ingredients may increase cordycepin production and bioconversion during C. militaris fermentation. We observed that porcine liver extracts increased cordycepin production. In addition, air supply (2 h/d) significantly increased the cordycepin level in surface liquid-cultured C...
January 2018: Journal of Food and Drug Analysis
Yibo Huang, Yujiro Higuchi, Takashi Kinoshita, Ai Mitani, Yasunari Eshima, Kaoru Takegawa
Endo-β-N-acetylglucosaminidase (ENGase) catalyzes hydrolysis of N-linked oligosaccharides. Although many ENGases have been characterized from various organisms, so far no fucose-containing oligosaccharides-specific ENGase has been identified in any organism. Here, we screened soil samples, using dansyl chloride (Dns)-labeled sialylglycan (Dns-SG) as a substrate, and discovered a strain that exhibits ENGase activity in the culture supernatant; this strain, named here as strain HMA12, was identified as a Sphingobacterium species by 16S ribosomal RNA gene analysis...
January 10, 2018: Scientific Reports
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