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https://www.readbyqxmd.com/read/29055284/multiple-sampling-and-saf-fixative-triple-faeces-testing-for-dysbiosis-and-pathogenic-infections-of-the-gastrointestinal-tract-case-report
#1
Johanna Mauss, Michelle Retz, Roger Dilts, Jeffrey Langland
The classic presentation of acute gastrointestinal (GI) parasitic infection including fever, diarrhea, nausea, and/or abdominal pain is not always a reliable measure. In these cases, parasitic infections require sensitive stool testing to be identified and treated effectively. The standard practice for stool analysis typically requires 3 separate stool specimens to be collected on 3 separate days to overcome the possibility of misdiagnosis due to intermittent shedding. Nonetheless, this practice is not often utilized due to very short periods of sample viability, which often leads to patient misdiagnosis...
October 21, 2017: Alternative Therapies in Health and Medicine
https://www.readbyqxmd.com/read/29055282/do-developmental-temperatures-affect-redox-level-and-lifespan-in-c-elegans-through-upregulation-of-peroxiredoxin
#2
Dylan Henderson, Christian Huebner, Moses Markowitz, Nicole Taube, Zachary M Harvanek, Ursula Jakob, Daniela Knoefler
Lifespan in poikilothermic organisms, such as Caenorhabditis elegans, can be substantially increased simply by decreasing growth temperature. To gain insights into the mechanistic underpinnings of this effect, we investigated the effects of temperature in development and adulthood on C. elegans lifespan. We found that worms exposed to 25°C during development and shifted to 15°C in adulthood exhibited an even longer lifespan than animals constantly kept at 15°C. Analysis of the in vivo redox status demonstrated that at 25°C, C...
October 7, 2017: Redox Biology
https://www.readbyqxmd.com/read/29055250/clinical-characteristics-of-patients-with-diagnostic-uncertainty-of-major-depressive-disorder
#3
Taichi Mogi, Hiroyuki Toda, Aihide Yoshino
BACKGROUND: Physicians do not always confidently diagnose psychiatric disorders. The present study was conducted to identify the clinical characteristics of patients in whom a definitive diagnosis of major depressive disorder (MDD) could not be established. METHODS: The participants were 199 consecutive outpatients with MDD, who were comprehensively diagnosed using the Mini International Neuropsychiatric Interview (MINI). The physician in charge of each patient quantified his/her sense of self-confidence in diagnosing the patient with MDD using the self-rating questionnaire in which the score ranged from 1 (not at all confident) to 5 (definitely MDD)...
October 16, 2017: Asian Journal of Psychiatry
https://www.readbyqxmd.com/read/29055217/trends-in-and-factors-affecting-the-observed-levels-of-urinary-inorganic-and-total-blood-mercury-among-us-children-adolescents-adults-and-senior-citizens-over-2005-2012
#4
Ram B Jain
Data from National Health and Nutrition Examination Survey (NHANES) for 2005-2012 were used to (i) study adjusted and unadjusted trends in the levels of urinary inorganic mercury (UIHG) and total blood mercury (TBHG) and (ii) factors that affect the observed levels of UIHG and TBHG among children aged 1-5 (CHLD15) and 6-11 (CHLD611) years, adolescents aged 12-19 years (ADOL), adults aged 20-64 years (ADLT), and senior citizens aged >=65 years (SNR). Decrease in adjusted levels of UIHG for each 2-year NHANES cycle for CHLD611 was 0...
September 6, 2017: Environmental Toxicology and Pharmacology
https://www.readbyqxmd.com/read/29054758/cystic-fibrosis-transmembrane-regulator-haplotypes-in-households-of-patients-with-cystic-fibrosis
#5
Daniela Tenório Furgeri, Fernando Augusto Lima Marson, Cyntia Arivabeni Araújo Correia, José Dirceu Ribeiro, Carmen Sílvia Bertuzzo
INTRODUCTION: Nearly 2000 mutations in the cystic fibrosis transmembrane regulator (CFTR) gene have been reported. The F508del mutation occurs in approximately 50-65% of patients with cystic fibrosis (CF). However, molecular diagnosis is not always possible. Therefore, silent polymorphisms can be used to label the mutant allele in households of patients with CF. OBJECTIVE: To verify the haplotypes of four polymorphisms at the CFTR locus in households of patients with CF for pre-fertilization, pre-implantation, and prenatal indirect mutation diagnosis to provide better genetic counseling for families and patients with CF and to associate the genotypes/haplotypes with the F508del mutation screening...
October 17, 2017: Gene
https://www.readbyqxmd.com/read/29054752/esophageal-lesions-following-button-battery-ingestion-in-children-analysis-of-causes-and-proposals-for-preventive-measures
#6
J Lahmar, C Célérier, E N Garabédian, V Couloigner, N Leboulanger, F Denoyelle
OBJECTIVES: To study recent cases of esophageal injury due to button-battery ingestion in children presenting in pediatric ENT emergency departments of the Paris area of France (Île-de-France region), in order to propose appropriate preventive measures. MATERIAL AND METHOD: A retrospective descriptive single-center study included all children under 15 years of age, presenting in pediatric ENT emergency departments between January 2008 and April 2014 for button-battery ingestion with esophageal impaction requiring emergency removal...
October 17, 2017: European Annals of Otorhinolaryngology, Head and Neck Diseases
https://www.readbyqxmd.com/read/29054643/polycyclic-aromatic-hydrocarbons-in-the-urban-atmosphere-of-nepal-distribution-sources-seasonal-trends-and-cancer-risk
#7
Balram Pokhrel, Ping Gong, Xiaoping Wang, Chuanfei Wang, Shaoping Gao
Atmospheric polycyclic aromatic hydrocarbons (PAHs) in urban areas have always been a global concern, as these areas are considered to be the source region. Despite studies on the concentrations of PAHs in water, soils and sediments, knowledge of the distribution patterns, seasonality and sources of PAHs in urban areas of Nepal remains limited. In this study, polyurethane foam passive air samplers were used to measure gas-phase PAH concentrations over different land types in three major cities of Nepal-namely, Kathmandu (the capital) and Pokhara (both densely populated cities), and Hetauda (an agricultural city)...
October 17, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/29054642/optical-properties-of-algogenic-organic-matter-within-the-growth-period-of-chlorella-sp-and-predicting-their-disinfection-by-product-formation
#8
Lap-Cuong Hua, Jr-Lin Lin, Ming-Yang Syue, Chihpin Huang, Pei-Chung Chen
Algogenic organic matter (AOM) in eutrophic waters is a well-known precursor to disinfection by-product (DBP) formation in drinking water. This purpose of this study is (i) to characterize the optical properties of AOM origins, including intra- (IOM) and extra-cellular organic matter (EOM), derived from Chlorella sp. growth as precursors to two major carbonaceous DBPs (C-DBPs), trihalomethanes (THMs) and haloacetic acids (HAAs) and (ii) to correlate these optical properties with THM and HAA formation potential (FP) in order to predict DBP formation...
October 17, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/29053994/dietary-resveratrol-confers-apoptotic-resistance-to-oxidative-stress-in-myoblasts
#9
Satoshi Haramizu, Shinichi Asano, David C Butler, David A Stanton, Ameena Hajira, Junaith S Mohamed, Stephen E Alway
High levels of reactive oxygen species (ROS) contribute to muscle cell death in aging and disuse. We have previously found that resveratrol can reduce oxidative stress in response to aging and hindlimb unloading in rodents in vivo, but it was not known if resveratrol would protect muscle stem cells during repair or regeneration when oxidative stress is high. To test the protective role of resveratrol on muscle stem cells directly, we treated the C2C12 mouse myoblast cell line with moderate (100 μM) or very high (1 mM) levels of H2O2 in the presence or absence of resveratrol...
August 24, 2017: Journal of Nutritional Biochemistry
https://www.readbyqxmd.com/read/29053977/suicidal-ideation-declines-with-improvement-in-the-subjective-symptoms-of-major-depression
#10
John G Keilp, Steven P Ellis, Marianne Gorlyn, Ainsley K Burke, Maria A Oquendo, J John Mann, Michael F Grunebaum
BACKGROUND: Suicidal ideation appears to be more strongly associated with subjective rather than neurovegetative symptoms of depression. Effective treatment, then, should produce reductions in suicidal ideation to the degree that these subjective symptoms are alleviated relative to treatment effects on other symptoms. METHODS: In a randomized clinical trial comparing paroxetine and bupropion for treatment of depression in patients with either suicidal ideation or past attempt, depression severity and suicidal ideation were assessed weekly during the 8-week study...
September 19, 2017: Journal of Affective Disorders
https://www.readbyqxmd.com/read/29053583/body-weight-cycling-with-identical-diet-composition-does-not-affect-energy-balance-and-has-no-adverse-effect-on-metabolic-health-parameters
#11
Inge F Palm, Rianne G A E Schram, Hans J M Swarts, Evert M van Schothorst, Jaap Keijer
BACKGROUND: Body weight (BW) cycling, the yo-yo effect, is generally thought to have adverse effects on human metabolic health. However, human and animal experiments are limited in number and do not provide clear answers, partly due to large variations in experimental design, parameters measured, and definitions of BW cycling. Here, we examined the effect of repetitive BW cycling versus single- and non-cycling control groups, without alterations in diet composition, on steady state BW and metabolic parameters...
October 20, 2017: Nutrients
https://www.readbyqxmd.com/read/29053406/super-resolution-nanoscopy-imaging-applied-to-dna-double-strand-breaks
#12
Sofia D'Abrantes, Sarah Gratton, Pamela Reynolds, Verena Kriechbaumer, Joseph McKenna, Stephen Barnard, Dave Clarke, Stanley W Botchway
Genomic deoxyribonucleic acid (DNA) is continuously being damaged by endogenous processes such as metabolism or by exogenous events such as radiation. The specific phosphorylation of histone H2AX on serine residue 139, described as γ-H2AX, is an excellent indicator or marker of DNA double-strand breaks (DSBs). The yield of γ-H2AX (foci) is shown to have some correlation with the dose of radiation or other DSB-causing agents. However, there is some discrepancy in the DNA DSB foci yield among imaging and other methods such as gel electrophoresis...
October 20, 2017: Radiation Research
https://www.readbyqxmd.com/read/29053256/electrochemical-conversion-of-fe3o4-magnetic-nanoparticles-to-electroactive-prussian-blue-analogues-for-novel-self-sacrificial-label-biosensing-of-avian-influenza-virus-h5n1
#13
Qi Zhang, Lingyan Li, Zhaohui Qiao, Chunyang Lei, Yingchun Fu, Qingji Xie, Shouzhuo Yao, Yanbin Li, Yibin Ying
A serious impetus always exists to exploit new methods to enrich the prospect of nanomaterials. Here, we report electrochemical conversion (ECC) of magnetic nanoparticles (MNPs) to electroactive Prussian blue (PB) analogues accompanying with multiple interfacial effects and its exploitation for novel label self-sacrificial biosensing strategy. The ECC method involves a high potential step to create strong acidic condition by splitting H2O to release Fe3+ from the MNPs, and then a low potential step leading to the reduction of co-existing K3Fe(CN)6 and Fe3+ to K4Fe(CN)6 and Fe2+, respectively, which react to form PB analogues...
October 20, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/29052958/whole-genome-re-sequencing-reveals-evolutionary-patterns-of-sacred-lotus-nelumbo-nucifera
#14
Longyu Huang, Mei Yang, Ling Li, Hui Li, Dong Yang, Tao Shi, Pingfang Yang
Sacred lotus (Nelumbo nucifera or lotus) is an important aquatic plant in horticulture and ecosystem. As a foundation for exploring genomic variationand evolution among different germplasms, we re-sequenced 19 individuals from three cultivated temperate lotus subgroups (rhizome lotus, seed lotus and flower lotus),one wild temperate lotus subgroup (wild lotus), one tropical lotus group (Thai lotus) and outgroup (Nelumbo lutea). Through genetic diversity and polymorphism analysis by non-missing SNP sites widely distributed in the whole genome, weconfirmed that wild lotus and Thai lotus exhibited greater differentiation with a higher genomic diversity compared to cultivated lotus...
October 20, 2017: Journal of Integrative Plant Biology
https://www.readbyqxmd.com/read/29052557/determination-of-volatile-organic-compounds-in-exhaled-breath-of-heart-failure-patients-by-needle-trap-micro-extraction-coupled-with-gas-chromatography-tandem-mass-spectrometry
#15
Denise Biagini, Tommaso Lomonaco, Silvia Ghimenti, Francesca Giuseppa Bellagambi, Massimo Onor, Maria Chiara Scali, Valentina Barletta, Mario Marzilli, Pietro Salvo, Maria Giovanna Trivella, Roger Fuoco, Fabio Di Francesco
The analytical performances of needle trap micro-extraction (NTME) coupled with gas chromatography tandem mass spectrometry were evaluated by analyzing a mixture of twenty-two representative breath VOCs belonging to different chemical classes (i.e. hydrocarbons, ketones, aldehydes, aromatics and sulfurs). NTME is an emerging technique that guarantees detection limits in pptv range by pre-concentrating low volumes of sample, and it is particularly suitable for breath analysis. For most VOCs, detection limits between 20 and 500 pptv were obtained by pre-concentrating 25 mL of a humidified standard gas mixture at a flow rate of 15 mL/min...
October 20, 2017: Journal of Breath Research
https://www.readbyqxmd.com/read/29052454/surgical-ethics-today-and-tomorrow
#16
Robert M Sade, Minoo N Kavarana
Ethical behavior has always been deeply ingrained in surgical culture, but ethical deliberation has only recently become an important component of cardiac surgical practice. In our earlier review, we covered a range of issues including several related to informed consent, conflict of interest, professional self-regulation and innovation, among many others. This update covers several topics of interest to cardiac surgeons and cardiologists, focusing on controversial issues specific to the practice of cardiothoracic surgery: informed consent, relations with hospitals and euthanasia and physician-assisted suicide...
October 20, 2017: Future Cardiology
https://www.readbyqxmd.com/read/29052320/altrenogest-treatment-before-weaning-improves-litter-size-in-sows
#17
T P Lopes, A Bolarín, E A Martínez, J Roca
The study aimed to assess whether altrenogest treatment, fed before weaning (from -8 to -2 days), could improve fertility of sows showing reproductive seasonality. Ninety sows (50 in winter-spring [WS] and 40 in summer-autumn [SA]) were randomly selected and assigned to control (C; 27 in WS and 20 in SA) or altrenogest treatment (A; 23 in WS and 20 in SA) groups. The diameter and number of ovarian follicles were transrectally scanned at the onset of oestrus. Oestrus was evaluated twice daily from weaning to day 8 post-weaning...
October 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/29052241/symbol-grounding-without-direct-experience-do-words-inherit-sensorimotor-activation-from-purely-linguistic-context
#18
Fritz Günther, Carolin Dudschig, Barbara Kaup
Theories of embodied cognition assume that concepts are grounded in non-linguistic, sensorimotor experience. In support of this assumption, previous studies have shown that upwards response movements are faster than downwards movements after participants have been presented with words whose referents are typically located in the upper vertical space (and vice versa for downwards responses). This is taken as evidence that processing these words reactivates sensorimotor experiential traces. This congruency effect was also found for novel words, after participants learned these words as labels for novel objects that they encountered either in their upper or lower visual field...
October 19, 2017: Cognitive Science
https://www.readbyqxmd.com/read/29052046/headache-and-pregnancy-a-systematic-review
#19
A Negro, Z Delaruelle, T A Ivanova, S Khan, R Ornello, B Raffaelli, A Terrin, U Reuter, D D Mitsikostas
This systematic review summarizes the existing data on headache and pregnancy with a scope on clinical headache phenotypes, treatment of headaches in pregnancy and effects of headache medications on the child during pregnancy and breastfeeding, headache related complications, and diagnostics of headache in pregnancy. Headache during pregnancy can be both primary and secondary, and in the last case can be a symptom of a life-threatening condition. The most common secondary headaches are stroke, cerebral venous thrombosis, subarachnoid hemorrhage, pituitary tumor, choriocarcinoma, eclampsia, preeclampsia, idiopathic intracranial hypertension, and reversible cerebral vasoconstriction syndrome...
October 19, 2017: Journal of Headache and Pain
https://www.readbyqxmd.com/read/29051960/comment-on-relating-side-chain-organization-of-pnipam-with-its-conformation-in-aqueous-methanol-by-d-mukherji-m-wagner-m-d-watson-s-winzen-t-e-de-oliveira-c-m-marques-and-k-kremer-soft-matter-2016-12-7995
#20
Andrea Pica, Giuseppe Graziano
In a recent article, Kremer and co-workers have combined NMR measurements and very long, all-atom MD simulations to strengthen their original claim that PNIPAM cononsolvency in water-methanol solutions is driven by the ability of MeOH molecules to bridge different monomers far away along the polymeric chain. In this comment, the results presented by Kremer and co-workers are reviewed, analyzed, and questioned regarding their ability to provide support to the bridging mechanism. Here, some pieces of evidence are provided to show that: (1) the solvent-excluded volume effect plays always a fundamental role in polymer collapse; (2) PNIPAM cononsolvency is caused by the geometric-energetic frustration experienced by the polymer when it can interact with both water and methanol molecules at the same time...
October 20, 2017: Soft Matter
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