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https://www.readbyqxmd.com/read/28424002/enzyme-linked-immunosorbent-assays-for-coproantigen-detection-of-ancylostoma-caninum-and-toxocara-canis-in-dogs-and-toxocara-cati-in-cats
#1
David A Elsemore, Jinming Geng, Jennifer Cote, Rita Hanna, Araceli Lucio-Forster, Dwight D Bowman
We report the development and field validation of 2 ELISAs for the detection of Ancylostoma caninum or Toxocara canis coproantigens in the feces of dogs with experimental and natural infections, and evidence of cross-reactivity with respective feline counterparts. A. caninum-specific coproantigens were detected in feces of experimentally infected dogs starting at 9 d post-infection (dpi), whereas eggs were not seen until 23 dpi. T. canis-specific coproantigens were detected in 3 of 5 experimentally infected dogs by 31 dpi, and 4 of the 5 animals by 38 dpi...
April 1, 2017: Journal of Veterinary Diagnostic Investigation
https://www.readbyqxmd.com/read/28399176/pathological-findings-in-the-red-fox-vulpes-vulpes-stone-marten-martes-foina-and-raccoon-dog-nyctereutes-procyonoides-with-special-emphasis-on-infectious-and-zoonotic-agents-in-northern-germany
#2
Charlotte Lempp, Nicole Jungwirth, Miguel L Grilo, Anja Reckendorf, Arlena Ulrich, Abbo van Neer, Rogier Bodewes, Vanessa M Pfankuche, Christian Bauer, Albert D M E Osterhaus, Wolfgang Baumgärtner, Ursula Siebert
Anthropogenic landscape changes contributed to the reduction of availability of habitats to wild animals. Hence, the presence of wild terrestrial carnivores in urban and peri-urban sites has increased considerably over the years implying an increased risk of interspecies spillover of infectious diseases and the transmission of zoonoses. The present study provides a detailed characterisation of the health status of the red fox (Vulpes vulpes), stone marten (Martes foina) and raccoon dog (Nyctereutes procyonoides) in their natural rural and peri-urban habitats in Schleswig-Holstein, Germany between November 2013 and January 2016 with focus on zoonoses and infectious diseases that are potentially threatening to other wildlife or domestic animal species...
2017: PloS One
https://www.readbyqxmd.com/read/28397316/proposed-expansion-margins-for-planning-organ-at-risk-volume-for-lenses-during-radiation-therapy-of-the-nasal-cavity-in-dogs-and-cats
#3
Zaki Jafry, Arnon Gal, Andre Fleck, Johnson Darko, Valerie J Poirier
Radiation therapy protocols for the feline or canine nasal cavity can damage epithelial cells of the posterior pole of the lens and lead to the development of cataracts. Aims of this retrospective, descriptive study were to calculate movements of the lens during radiation therapy of the nasal cavity in a sample of cats and dogs, and to propose species-specific expansion margins for planning organ at risk volume (PRV) to minimize radiation doses to the lens. All included patients were immobilized with an indexed bite block and positioned in a vacuum positioning cushion for head irradiation...
April 11, 2017: Veterinary Radiology & Ultrasound
https://www.readbyqxmd.com/read/28378987/stray-dogs-and-cats-as-potential-sources-of-soil-contamination-with-zoonotic-parasites
#4
Katarzyna Szwabe, Joanna Blaszkowska
INTRODUCTION AND OBJECTIVE: The main source of many zoonoses is soil contaminated with feline and canine faeces. Thus, the aim of this study was to estimate the prevalence of intestinal parasites in stray dogs and cats adopted in Lodz shelter (Poland). MATERIALS AND METHODS: In total, 163 faecal samples were collected from 95 dogs and 68 cats from 2011 to 2012. The samples were processed by sedimentation techniques using Mini Parasep®SF. RESULTS: Six parasite genera belonging to protozoa, cestoda, and nematoda, were found in dogs, while eight were found in cats...
March 22, 2017: Annals of Agricultural and Environmental Medicine: AAEM
https://www.readbyqxmd.com/read/28369886/efficacy-of-feline-anti-parvovirus-antibodies-in-the-treatment-of-canine-parvovirus-infection
#5
M Gerlach, A L Proksch, S Unterer, S Speck, U Truyen, K Hartmann
OBJECTIVE: This prospective, randomised, placebo-controlled, double-blinded study aimed to evaluate efficacy of commercially available feline anti-parvovirus antibodies in dogs with canine parvovirus infection. METHODS: First, cross-protection of feline panleukopenia virus antibodies against canine parvovirus was evaluated in vitro. In the subsequent prospective clinical trial, 31 dogs with clinical signs of canine parvovirus infection and a positive faecal canine parvovirus polymerase chain reaction were randomly assigned to a group receiving feline panleukopenia virus antibodies (n=15) or placebo (n=16)...
March 31, 2017: Journal of Small Animal Practice
https://www.readbyqxmd.com/read/28355954/evaluation-of-an-automated-enzyme-linked-fluorescent-assay-for-thyroxine-measurement-in-cat-and-dog-sera
#6
Rouven Anderson, Ralf Mueller, Sven Reese, Astrid Wehner
Measurement of total thyroxine (T4) is the first testing step in the work-up of thyroid disease in small animals. We evaluated an enzyme-linked fluorescent assay (ELFA) as an in-house method to measure T4 in cats and dogs. We compared the T4 concentration in sera of 122 cats and 176 dogs measured by the ELFA with an enzyme immunoassay (EIA) to assess the concordance of the 2 methods. Bias of the ELFA in cats was -11.4% and in dogs 1.4%. Using Bland-Altman plots, limits of agreement were -81.5 to 58.7% in cats and -71...
March 1, 2017: Journal of Veterinary Diagnostic Investigation
https://www.readbyqxmd.com/read/28346123/panfungal-polymerase-chain-reaction-for-identification-of-fungal-pathogens-in-formalin-fixed-animal-tissues
#7
Courtney Meason-Smith, Erin E Edwards, Caitlin E Older, Mackenzie Branco, Laura K Bryan, Sara D Lawhon, Jan S Suchodolski, Gabriel Gomez, Joanne Mansell, Aline Rodrigues Hoffmann
Identification of fungal organisms often poses a problem for pathologists because the histomorphology of some fungal organisms is not specific, fresh tissues may not be available, and isolation and identification in culture may take a long time. The purpose of this study was to validate the use of panfungal polymerase chain reaction (PCR) to identify fungal organisms from formalin-fixed paraffin-embedded (FFPE) tissues. Formalin-fixed paraffin-embedded curls were tested from 128 blocks containing canine, feline, equine, and bovine tissues with cutaneous, nasal, pulmonary, and systemic fungal infections, identified by the presence of fungi in histologic sections...
January 1, 2017: Veterinary Pathology
https://www.readbyqxmd.com/read/28319274/intraoperative-optical-coherence-tomography-for-soft-tissue-sarcoma-differentiation-and-margin-identification
#8
Kelly J Mesa, Laura E Selmic, Paritosh Pande, Guillermo L Monroy, Jennifer Reagan, Jonathan Samuelson, Elizabeth Driskell, Joanne Li, Marina Marjanovic, Eric J Chaney, Stephen A Boppart
BACKGROUND AND OBJECTIVE: Sarcomas are rare but highly aggressive tumors, and local recurrence after surgical excision can occur in up to 50% cases. Therefore, there is a strong clinical need for accurate tissue differentiation and margin assessment to reduce incomplete resection and local recurrence. The purpose of this study was to investigate the use of optical coherence tomography (OCT) and a novel image texture-based processing algorithm to differentiate sarcoma from muscle and adipose tissue...
March 2017: Lasers in Surgery and Medicine
https://www.readbyqxmd.com/read/28317614/snap-tests-for-pancreatitis-in-dogs-and-cats-snap-canine-pancreatic-lipase-and-snap-feline-pancreatic-lipase
#9
REVIEW
Panagiotis G Xenoulis, Jörg M Steiner
A clinical diagnosis of pancreatitis in dogs and cats can be challenging. Several diagnostic modalities have been evaluated over the years for the diagnosis of canine and feline pancreatitis, but most of these modalities have been shown to be of limited clinical use because of poor performance, limited availability, or because they are invasive, or all of these. Assays for the measurement of pancreatic lipase (PL) immunoreactivity [Specific canine PL (Spec cPL) in dogs and Specific feline PL (Spec fPL) in cats] were first developed approximately 15 years ago, and studies have shown that they are currently the serum tests of choice for the evaluation of canine and feline patients, respectively, suspected of having pancreatitis...
December 2016: Topics in Companion Animal Medicine
https://www.readbyqxmd.com/read/28288759/feline-and-canine-leishmaniosis-and-other-vector-borne-diseases-in-the-aeolian-islands-pathogen-and-vector-circulation-in-a-confined-environment
#10
Domenico Otranto, Ettore Napoli, Maria Stefania Latrofa, Giada Annoscia, Viviana Domenica Tarallo, Grazia Greco, Eleonora Lorusso, Laura Gulotta, Luigi Falsone, Fabrizio Solari Basano, Maria Grazia Pennisi, Katrin Deuster, Gioia Capelli, Filipe Dantas-Torres, Emanuele Brianti
Vector-borne diseases (VBDs) are prevalently investigated in dogs. Studies on feline VBDs are scant, though feline leishmaniosis (FeL) is increasingly recognised as a disease of cats in endemic areas. Comprehensive investigations on the distribution of VBDs in populations of cats and dogs living in relatively small geographical areas, such as islands, are currently lacking. In this study the prevalence of Leishmania infantum and other VBD pathogens was assessed in cohorts of cats and dogs living in the Aeolian Islands...
March 15, 2017: Veterinary Parasitology
https://www.readbyqxmd.com/read/28285588/prevalence-and-molecular-characterization-of-canine-and-feline-hemotropic-mycoplasmas-hemoplasmas-in-northern-italy
#11
Silvia Ravagnan, Erika Carli, Eleonora Piseddu, Graziana Da Rold, Elena Porcellato, Claudia Zanardello, Antonio Carminato, Marta Vascellari, Gioia Capelli
BACKGROUND: Hemotropic mycoplasmas (hemoplasmas), the agents of infectious anemia, have been reported in dogs and cats. Little data are available on hemoplasma infections in Italy. The aim of this study was to evaluate the species of hemoplasmas and their prevalence in dogs and cats of northern Italy. METHODS: Blood samples were obtained from 117 candidate blood donor dogs, 278 free-roaming dogs and 227 free-roaming cats in 2014 and 2015. Samples were first screened for hemoplasmas with a SYBR green real time PCR...
March 13, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/28264705/improved-molecular-detection-of-babesia-infections-in-animals-using-a-novel-quantitative-real-time-pcr-diagnostic-assay-targeting-mitochondrial-dna
#12
Barbara A Qurollo, Nikole R Archer, Megan E Schreeg, Henry S Marr, Adam J Birkenheuer, Kaitlin N Haney, Brittany S Thomas, Edward B Breitschwerdt
BACKGROUND: Babesiosis is a protozoal, tick transmitted disease found worldwide in humans, wildlife and domesticated animals. Commonly used approaches to diagnose babesiosis include microscopic examination of peripheral blood smears, detection of circulating antibodies and PCR. To screen and differentiate canine Babesia infections many PCR assays amplify the 18S rRNA gene. These sequences contain hypervariable regions flanked by highly conserved regions allowing for amplification of a broad-range of Babesia spp...
March 7, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/28255929/rotavirus-i-in-feces-of-a-cat-with-diarrhea
#13
Tung G Phan, Christian M Leutenegger, Roxanne Chan, Eric Delwart
A divergent rotavirus I was detected using viral metagenomics in the feces of a cat with diarrhea. The eleven segments of rotavirus I strain Felis catus encoded non-structural and structural proteins with amino acid identities ranging from 25 to 79% to the only two currently sequenced members of that viral species both derived from canine feces. No other eukaryotic viral sequences nor bacterial and protozoan pathogens were detected in this fecal sample suggesting the involvement of rotavirus I in feline diarrhea...
March 2, 2017: Virus Genes
https://www.readbyqxmd.com/read/28235458/no-molecular-epidemiological-evidence-supporting-household-transmission-of-zoonotic-giardia-duodenalis-and-cryptosporidium-spp-from-pet-dogs-and-cats-in-the-province-of-%C3%A3-lava-northern-spain
#14
Aida de Lucio, Begoña Bailo, María Aguilera, Guillermo A Cardona, Juan C Fernández-Crespo, David Carmena
The role of pet dogs and cats as suitable source of human infections by the diarrheagenic protozoan parasites Giardia duodenalis and Cryptosporidium spp. has been a topic of intense debate for long time and still remains a largely unsolved problem. In this cross-sectional molecular epidemiological survey we attempted to investigate whether zoonotic (or zooanthroponotic) disease transmission was occurring among humans and domestic dogs and cats sharing the same spatial and temporal setting in both rural and urban areas of the province of Álava, Northern Spain...
February 21, 2017: Acta Tropica
https://www.readbyqxmd.com/read/28219812/detection-and-molecular-diversity-of-giardia-duodenalis-and-cryptosporidium-spp-in-sheltered-dogs-and-cats-in-northern-spain
#15
Horacio Gil, Lourdes Cano, Aida de Lucio, Begoña Bailo, Marta Hernández de Mingo, Guillermo A Cardona, José A Fernández-Basterra, Juan Aramburu-Aguirre, Nuria López-Molina, David Carmena
Domestic dogs and cats may act as natural reservoirs of a large number of zoonotic pathogens, including the enteric parasites Giardia duodenalis and Cryptosporidium spp., the most relevant protozoan species causing gastrointestinal disease worldwide. A cross-sectional epidemiological study aiming to assess the prevalence and molecular diversity of G. duodenalis and Cryptosporidium spp. was conducted in an animal rescue centre in the province of Álava (Northern Spain). A total of 194 and 65 faecal dropping samples from individual dogs and cats, respectively, were collected between November 2013 and June 2016...
February 17, 2017: Infection, Genetics and Evolution
https://www.readbyqxmd.com/read/28216682/use-of-canine-sourced-platelet-rich-plasma-in-a-feline-contaminated-cutaneous-wound
#16
Francesco Gemignani, Anna Perazzi, Ilaria Iacopetti
A 4-year-old neutered female domestic shorthaired cat was treated with canine sourced platelet-rich plasma at the Veterinary Hospital at University of Padua for a large skin defect on the left lateral neck region. The wound healed completely within 20 days and no adverse reaction was observed during the healing process.
February 2017: Canadian Veterinary Journal. la Revue Vétérinaire Canadienne
https://www.readbyqxmd.com/read/28214332/comparison-of-several-white-matter-tracts-in-feline-and-canine-brain-by-using-magnetic-resonance-diffusion-tensor-imaging
#17
Olivier Jacqmot, Bert Van Thielen, Alex Michotte, Inneke Willekens, Filip Verhelle, Peter Goossens, Filip De Ridder, Jan Pieter Clarys, Anne Vanbinst, Cindy Peleman, Johan de Mey
Recently, we published a first anatomical Diffusion Tensor Imaging (DTI) atlas regarding white matter tracts in the canine brain. The purpose of this study was to show the significance of DTI in the revelation of the white matter fibres in the feline brain (i.e. to obtain an anatomical DTI atlas of images) and to descriptively compare these to previously obtained white matter fibre images of the canine brain. DTI MR Images of 4 cats euthanised for reasons other than neurological disorders were obtained with a 3 T system...
February 18, 2017: Anatomical Record: Advances in Integrative Anatomy and Evolutionary Biology
https://www.readbyqxmd.com/read/28154463/association-of-gingivitis-with-dental-calculus-thickness-or-dental-calculus-coverage-and-subgingival-bacteria-in-feline-leukemia-virus-and-feline-immunodeficiency-virus-negative-cats
#18
Naris Thengchaisri, Jörg M Steiner, Jan S Suchodolski, Panpicha Sattasathuchana
Periodontal disease is the most common oral disease in cats. The objectives of this study were to determine the relationships between gingivitis and dental calculus thickness (DCT), or dental calculus coverage (DCC); determine the association of gingivitis scores and types of oral bacteria; and to evaluate bacterial co-infection in cats with periodontal disease. Twelve cats that were not infected with feline leukemia or feline immunodeficiency viruses were enrolled in the study. Gingivitis, DCT, and DCC were scored and recorded...
January 2017: Canadian Journal of Veterinary Research, Revue Canadienne de Recherche Vétérinaire
https://www.readbyqxmd.com/read/28146301/simultaneous-determination-of-mycophenolic-acid-and-its-glucuronide-and-glycoside-derivatives-in-canine-and-feline-plasma-by-uhplc-uv
#19
Sol Maiam Rivera, Jeniffer E Slovak, Michael H Court, Nicolas F Villarino
Cats and dogs can suffer from multiple auto-immune diseases. Mycophenolic acid (MPA) is a potentially useful immunosuppressant drug in cats and dogs, because of its well-documented efficacy in controlling autoimmune disease in humans. However, the pharmacokinetics and pharmacodynamics in these species remains to be determined. We have developed and validated a sensitive, precise, accurate, and reproducible method that provides consistent quantification of MPA and its major derivatives, MPA phenol glucoside and MPA phenol glucuronide in canine and feline plasma using ultra-high-pressure liquid chromatography coupled to an ultraviolet detector...
February 1, 2017: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/28143510/gastropod-derived-haemocyte-extracellular-traps-entrap-metastrongyloid-larval-stages-of-angiostrongylus-vasorum-aelurostrongylus-abstrusus-and-troglostrongylus-brevior
#20
Malin K Lange, Felipe Penagos-Tabares, Tamara Muñoz-Caro, Ulrich Gärtner, Helena Mejer, Roland Schaper, Carlos Hermosilla, Anja Taubert
BACKGROUND: Phagocyte-derived extracellular traps (ETs) were recently demonstrated mainly in vertebrate hosts as an important effector mechanism against invading parasites. In the present study we aimed to characterize gastropod-derived invertebrate extracellular phagocyte trap (InEPT) formation in response to larval stages of important canine and feline metastrongyloid lungworms. Gastropod haemocytes were isolated from the slug species Arion lusitanicus and Limax maximus, and the snail Achatina fulica, and exposed to larval stages of Angiostrongylus vasorum, Aelurostrongylus abstrusus and Troglostrongylus brevior and investigated for gastropod-derived InEPT formation...
January 31, 2017: Parasites & Vectors
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