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https://www.readbyqxmd.com/read/29779898/oncostatin-m-and-kit-ligand-control-hematopoietic-stem-cell-fate-during-zebrafish-embryogenesis
#1
Christopher B Mahony, Corentin Pasche, Julien Y Bertrand
Understanding the molecular pathways controlling hematopoietic stem cell specification and expansion is a necessary milestone to perform regenerative medicine. Here, we used the zebrafish model to study the role of the ckit signaling pathway in this process. We show the importance of kitb/kitlgb signaling in the specification and expansion of hematopoietic stem cells (HSCs), in the hemogenic endothelium and caudal hematopoietic tissue (CHT), respectively. Moreover, we identified the zebrafish ortholog of Oncostatin M (osm) in the zebrafish genome...
May 10, 2018: Stem Cell Reports
https://www.readbyqxmd.com/read/29777134/zebrafish-vcap1x2-regulates-cardiac-contractility-and-proliferation-of-cardiomyocytes-and-epicardial-cells
#2
Fang-Chi Hsieh, Yu-Fen Lu, Ian Liau, Chien-Chang Chen, Chao-Min Cheng, Chung-Der Hsiao, Sheng-Ping L Hwang
Sarcomeric signaling complexes are important to sustain proper sarcomere structure and function, however, the mechanisms underlying these processes are not fully elucidated. In a gene trap experiment, we found that vascular cell adhesion protein 1 isoform X2 (VCAP1X2) mutant embryos displayed a dilated cardiomyopathy phenotype, including reduced cardiac contractility, enlarged ventricular chamber and thinned ventricular compact layer. Cardiomyocyte and epicardial cell proliferation was decreased in the mutant heart ventricle, as was the expression of pAKT and pERK...
May 18, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29757409/secretogranin-ii-plays-a-critical-role-in-zebrafish-neurovascular-modeling
#3
Binbin Tao, Hongling Hu, Kimberly Mitchell, Ji Chen, Haibo Jia, Zuoyan Zhu, Vance L Trudeau, Wei Hu
Secretoneurin (SN) is a neuropeptide derived from specific proteolytic processing of the precursor secretogranin II (SgII). In zebrafish and other teleosts, there are two paralogs named sgIIa and sgIIb. Our results showed that neurons expressing sgIIb were aligned with central arteries in the hindbrain, demonstrating a close neurovascular association. Both sgIIb-/- and sgIIa-/-/sgIIb-/- mutant embryos were defective in hindbrain central artery development due to impairment of migration and proliferation of central artery cells...
May 11, 2018: Journal of Molecular Cell Biology
https://www.readbyqxmd.com/read/29753018/ets-transcription-factor-etsrp-etv2-is-required-for-lymphangiogenesis-and-directly-regulates-vegfr3-flt4-expression
#4
Jennifer A Davis, Andrew L Koenig, Allison Lubert, Brendan Chestnut, Fang Liu, Sharina Palencia Desai, Tamara Winkler, Karolina Pociute, Kyunghee Choi, Saulius Sumanas
The molecular mechanisms initiating the formation of the lymphatic system, lymphangiogenesis, are still poorly understood. Here we have identified a novel role in lymphangiogenesis for an ETS transcription factor, Etv2/Etsrp, a known regulator of embryonic vascular development. Through the use of fully validated photoactivatable morpholinos we show that inducible Etv2 inhibition in zebrafish embryos at 1day post-fertilization (dpf) results in significant inhibition of lymphangiogenesis, while development of blood vessels is unaffected...
May 9, 2018: Developmental Biology
https://www.readbyqxmd.com/read/29752956/advanced-microscopy-to-elucidate-cardiovascular-injury-and-regeneration-4d-light-sheet-imaging
#5
REVIEW
Kyung In Baek, Yichen Ding, Chih-Chiang Chang, Megan Chang, René R Sevag Packard, Jeffrey J Hsu, Peng Fei, Tzung K Hsiai
The advent of 4-dimensional (4D) light-sheet fluorescence microscopy (LSFM) has provided an entry point for rapid image acquisition to uncover real-time cardiovascular structure and function with high axial resolution and minimal photo-bleaching/-toxicity. We hereby review the fundamental principles of our LSFM system to investigate cardiovascular morphogenesis and regeneration after injury. LSFM enables us to reveal the micro-circulation of blood cells in the zebrafish embryo and assess cardiac ventricular remodeling in response to chemotherapy-induced injury using an automated segmentation approach...
May 9, 2018: Progress in Biophysics and Molecular Biology
https://www.readbyqxmd.com/read/29744287/a-noncanonical-function-of-histidyl-trna-synthetase-inhibition-of-vascular-hyperbranching-during-zebrafish-development
#6
Rui Ni, Lingfei Luo
Histidyl-tRNA synthetase (Hars) catalyzes the ligation of histidine residues to cognate tRNA. Here, we demonstrate a noncanonical function of Hars in vascular development in zebrafish. We obtained a novel zebrafish cq34 mutant which exhibited hyperbranching of cranial and intersegmental blood vessels 48 h after fertilization. The gene responsible for this phenotype was identified as hars . We found the increased expression of cdh5 and vegfa in the hars cq34 mutant. Knockdown of cdh5 in the mutant reduced disordered connections of the hindbrain capillaries...
May 2018: FEBS Open Bio
https://www.readbyqxmd.com/read/29730311/sodium-tanshinone-iia-sulfonate-promotes-endothelial-integrity-via-regulating-ve-cadherin-dynamics-and-rhoa-rock-mediated-cellular-contractility-and-prevents-atorvastatin-induced-intracerebral-hemorrhage-in-zebrafish
#7
Zhong-Yan Zhou, Bin Huang, Shang Li, Xiao-Hui Huang, Jing-Yi Tang, Yiu Wa Kwan, Pui Man Hoi, Simon Ming-Yuen Lee
Impaired vascular integrity leads to serious cerebral vascular diseases such as intracerebral hemorrhage (ICH). In addition, high-dose statin therapy is suggested to cause increased ICH risk due to unclear effects of general inhibition of 3-hydroxy-3-methylglutaryl-CoA reductase (HMGCR) on the vascular system. Here we evaluated the protective effects of sodium tanshinone IIA sulfonate (STS), which has high efficacy and safety in clinical studies of ischemic stroke, by using atorvastatin (Ator) induced ICH zebrafish embryos and human umbilical vein endothelial cells (HUVECs)...
May 3, 2018: Toxicology and Applied Pharmacology
https://www.readbyqxmd.com/read/29724820/temporal-and-spatial-post-transcriptional-regulation-of-zebrafish-tie1-mrna-by-long-noncoding-rna-during-brain-vascular-assembly
#8
Tamjid A Chowdhury, Chris Koceja, Shahram Eisa-Beygi, Benjamin P Kleinstiver, Suresh N Kumar, Chien-Wei Lin, Keguo Li, Shubhangi Prabhudesai, J Keith Joung, Ramani Ramchandran
OBJECTIVE: Tie1 (tyrosine kinase containing immunoglobulin and epidermal growth factor homology 1), an endothelial and hematopoietic cell-specific receptor tyrosine kinase, is an important regulator of angiogenesis and critical for maintaining vascular integrity. The post-transcriptional regulation of tie1 mRNA expression is not understood, but it might partly explain Tie1's differential expression pattern in endothelium. Following up on our previous work that identified natural antisense transcripts from the tie1 locus- tie1 antisense ( tie1AS ), which regulates tie1 mRNA levels in zebrafish-we attempted to identify the mechanism of this regulation...
May 3, 2018: Arteriosclerosis, Thrombosis, and Vascular Biology
https://www.readbyqxmd.com/read/29721065/development-of-a-novel-albumin-based-and-maleimidopropionic-acid-conjugated-peptide-with-prolonged-half-life-and-increased-in-vivo-anti-tumor-efficacy
#9
Junnan Feng, Chuanke Zhao, Lixin Wang, Like Qu, Hua Zhu, Zhi Yang, Guo An, Huifang Tian, Chengchao Shou
Angiogenesis plays a critical role in tumor aggressiveness, and a lot of anti-angiogenic agents have been used in clinical therapy. The therapeutic efficacy of peptides are generally restricted by the short in vivo life-time, thus, we were interested in developing a novel albumin-based and maleimidopropionic acid-conjugated peptide to prolong the half-life and improve the anti-tumor effect. Methods: We developed a peptide F56 with a maleimidopropionic acid (MPA) at the C-terminal (denoted as F56-CM), which allows immediate and irreversible conjugation with serum albumin...
2018: Theranostics
https://www.readbyqxmd.com/read/29689360/a-systematic-toxicity-evaluation-of-cephalosporins-via-transcriptomics-in-zebrafish-and-in-silico-admet-studies
#10
Ying Han, Jingpu Zhang, Changqin Hu
Cephalosporins are beta-lactam antibiotics that are widely used in clinics in China. However, information on their toxicity to zebrafish is limited. This study reports that the toxicity effects of cephalosporins containing an N-methyltetrazolthio ring at the C-3 position (CNMTs) exposure on zebrafish were comparable to those predicted by in silico analysis. The effects of CNMTs on the mortality and malformation rate of zebrafish were concentration-dependent. The transcriptional levels of the has1 and cnnm2a genes, which are related to embryo development and absorption of Mg2+ in vivo, significantly changed...
April 21, 2018: Food and Chemical Toxicology
https://www.readbyqxmd.com/read/29684697/new-arene-ruthenium-ii-complexes-of-4%C3%A2-aryl%C3%A2-4h%C3%A2-naphthopyrans-with-anticancer-and-anti-vascular-activities
#11
Florian Schmitt, Jana Kasparkova, Viktor Brabec, Gerrit Begemann, Rainer Schobert, Bernhard Biersack
A series of four 2‑amino‑3‑cyano‑4‑(3/4‑pyridyl)‑4H‑benzo[h]chromenes 2a-d and their dichlorido(p‑cymene)ruthenium(II) complexes 3a-d were tested for antiproliferative, vascular-disruptive, anti-angiogenic and DNA-binding activity. The coordination of the 4‑pyridyl‑4H‑naphthopyrans 2 to ruthenium led to complexes with pleiotropic effects. Unlike the free ligands 2a-d, their ruthenium complexes 3a-d showed a significant affinity for DNA as demonstrated by electrophoretic mobility shift assays (EMSA) and ethidium bromide assays...
April 5, 2018: Journal of Inorganic Biochemistry
https://www.readbyqxmd.com/read/29670362/antiangiogenic-effects-of-aa-pme-on-huvecs-in-vitro-and-zebrafish-in-vivo
#12
Yue Jing, Gang Wang, Qi Xiao, Yachun Zhou, Yingjie Wei, Zhunan Gong
Angiogenesis plays a vital role in many physiological and pathological processes and several diseases are connected with its dysregulation. Asiatic acid (AA) has demonstrated anticancer properties and we suspect this might be attributable to an effect on angio-genesis. A modified derivative of AA, N-(2α,3β,23-acetoxyurs-12-en-28-oyl)-L-proline methyl ester (AA-PMe), has improved efficacy over its parent compound, but its effect on blood vessel development remains unclear. Methods: In this study, we investigated the antiangiogenic activity of AA and AA-PMe in zebrafish embryos and human umbilical vein endothelial cells (HUVECs)...
2018: OncoTargets and Therapy
https://www.readbyqxmd.com/read/29658397/inflammation-coagulation-response-and-thrombotic-effects-induced-by-silica-nanoparticles-in-zebrafish-embryos
#13
Junchao Duan, Shuang Liang, Yang Yu, Yang Li, Lijing Wang, Zehao Wu, Yueyue Chen, Mark R Miller, Zhiwei Sun
Nowadays, nanotechnology environmental health and safety (nanoEHS) is gaining attention. We previously found that silica nanoparticles (SiNPs) could induce vascular endothelial damage. However, the subsequent toxicologic response to SiNPs-induced endothelial damage was still largely unknown. In this study, we explored the inflammation-coagulation response and thrombotic effects of SiNPs in endothelial cells and zebrafish embryos. For in vitro study, swollen mitochondria and autophagosome were observed in ultrastructural analysis...
April 14, 2018: Nanotoxicology
https://www.readbyqxmd.com/read/29655679/the-extract-of-polygoni-cuspidati-rhizoma-et-radix-suppresses-the-vascular-endothelial-growth-factor-induced-angiogenesis
#14
Wei-Hui Hu, Gallant Kar-Lun Chan, Jian-Shu Lou, Qi-Yun Wu, Huai-You Wang, Ran Duan, Michael Yu-Tung Cheng, Tina Ting-Xia Dong, Karl Wah-Keung Tsim
BACKGROUND: Polygoni Cuspidati Rhizoma et Radix (PCRR; the root and rhizome of Polygonum cuspidatum Sieb. et Zucc) is a traditional Chinese medicine for the treatment of inflammation, hyperlipemia, favus, jaundice and scald. HYPOTHESIS/PURPOSE: The extract of PCRR inhibits vascular endothelial growth factor (VEGF)-induced angiogenesis. The hypothesis is supported by analysis of PCRR extract and investigation of pharmacological role and signaling mechanism of PCRR extract in regulating angiogenic responses...
March 15, 2018: Phytomedicine: International Journal of Phytotherapy and Phytopharmacology
https://www.readbyqxmd.com/read/29610928/brain-drains-new-insights-into-brain-clearance-pathways-from-lymphatic-biology
#15
REVIEW
Neil I Bower, Benjamin M Hogan
The lymphatic vasculature act as the drainage system for most of our tissues and organs, clearing interstitial fluid and waste and returning them to the blood circulation. This is not the case for the central nervous system (CNS), which is devoid of parenchymal lymphatic vessels. Nevertheless, the brain is responsible for 25% of the body's metabolism and only compromises 2% of the body's mass. This high metabolic load requires an efficient system to remove waste products and maintain homeostasis. Well-described mechanisms of waste clearance include phagocytic immune cell functions as well as perivascular fluid flow; however, the need for active drainage of waste from the brain is becoming increasingly appreciated...
April 2, 2018: Journal of Molecular Medicine: Official Organ of the "Gesellschaft Deutscher Naturforscher und Ärzte"
https://www.readbyqxmd.com/read/29596928/cardiac-safety-evaluation-in-zebrafish-and-in-silico-adme-prediction-of-cephalosporins-with-an-aminothiazoyl-ring-at-the-c-7-position
#16
Ying Han, Bo Chen, Jingpu Zhang, Changqin Hu
Systems toxicology approaches have been used as important tools in the drug discovery and medicine quality control processes. The aim of this study was to assess the pharmacokinetic and toxicity properties of cephalosporins with an aminothiazoyl ring at the C-7 position (CATRs). Cardiac toxicity of the compounds was assessed in zebrafish embryos, and it was determined that CATRs disturbed the formation and development of the heart in a dose-dependent manner. Differentially expressed genes (DEGs) related to the heart were also identified by transcriptome analysis, and co-DEGs were obtained in the protein-protein interaction (PPI) network...
March 26, 2018: Toxicology and Applied Pharmacology
https://www.readbyqxmd.com/read/29593015/an-apoo-pseudogene-on-chromosome-5q-is-associated-with-ldl-c-levels
#17
May E Montasser, Elizabeth A O'Hare, Xiaochun Wang, Alicia D Howard, Rebecca McFarland, James A Perry, Kathleen A Ryan, Kenneth Rice, Cashell E Jaquish, Alan R Shuldiner, Michael Miller, Braxton D Mitchell, Norann A Zaghloul, Yen-Pei C Chang
Background -Elevated levels of low-density lipoprotein cholesterol (LDL-C) are a major risk factor for CVD via its contribution to the development and progression of atherosclerotic lesions. While the genetic basis of LDL-C has been studied extensively, currently known genetic variants account for only ~20% of the variation in LDL-C levels. Methods -Through an array-based association analysis in 1,102 Amish subjects, we identified a variant strongly associated with LDL-C levels. Using a combination of genetic analyses, zebrafish models, and in in vitro experiments, we sought to identify the causal gene driving this association...
March 28, 2018: Circulation
https://www.readbyqxmd.com/read/29577856/blood-flow-regulates-zebrafish-caudal-vein-plexus-angiogenesis-by-erk5-klf2a-nos2b-signaling
#18
X Xie, T Zhou, Y Wang, H Chen, D Lei, L Huang, Y Wang, X Jin, T Sun, J Tan, T Yin, J Huang, H Gregersen, G Wang
Vascular network formation induced by angiogenesis plays an important role in many physiological and pathological processes. However, the role of blood flow and underlying mechanisms in vascular network formation, for example for the development of the caudal vein plexus (CVP), is poorly understood. In this study on tnnt2a-MO injection and chemical blood flow modulator treatment in zebrafish embryos, we demonstrated that decreased blood flow disrupted CVP formation. The hemodynamic force was quantitatively analyzed...
March 22, 2018: Current Molecular Medicine
https://www.readbyqxmd.com/read/29560968/dynamic-mapping-of-spontaneously-produced-h-2-s-in-the-entire-cell-space-and-in-live-animals-using-a-rationally-designed-molecular-switch
#19
Linlin Yang, Jun Zhao, Xinling Yu, Ruilong Zhang, Guangmei Han, Renyong Liu, Zhengjie Liu, Tingting Zhao, Ming-Yong Han, Zhongping Zhang
Hydrogen sulfide (H2S) is a key signaling molecule in the cytoprotection, vascular mediation and neurotransmission of living organisms. In-depth understanding of its production, trafficking, and transformation in cells is very important in the way H2S mediates cellular signal transductions and organism functions; it also motivates the development of H2S probes and imaging technologies. A fundamental challenge, however, is how to engineer probes with sensitivity and cellular penetrability that allow detection of spontaneous production of H2S in the entire cell space and live animals...
April 16, 2018: Analyst
https://www.readbyqxmd.com/read/29559522/aibp-protects-against-metabolic-abnormalities-and-atherosclerosis
#20
Dina A Schneider, Soo-Ho Choi, Colin Agatisa-Boyle, Laurence Zhu, Jungsu Kim, Jennifer Pattison, Dorothy D Sears, Philip L S M Gordts, Longhou Fang, Yury I Miller
Apolipoprotein A-I binding protein (AIBP) has been shown to augment cholesterol efflux from endothelial cells and macrophages. In zebrafish and mice, AIBP-mediated regulation of cholesterol levels in the plasma membrane of endothelial cells controls angiogenesis. The goal of this work was to evaluate metabolic changes and atherosclerosis in AIBP loss-of-function and gain-of-function animal studies. Here, we show that Apoa1bp-/- Ldlr-/- mice fed a high-cholesterol, high-fat diet had exacerbated weight gain, liver steatosis, glucose intolerance, hypercholesterolemia, hypertriglyceridemia, and larger atherosclerotic lesions compared with Ldlr-/- mice...
May 2018: Journal of Lipid Research
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