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https://www.readbyqxmd.com/read/28332618/the-chemokine-receptor-cxcr4-promotes-granuloma-formation-by-sustaining-a-mycobacteria-induced-angiogenesis-programme
#1
Vincenzo Torraca, Claudia Tulotta, B Ewa Snaar-Jagalska, Annemarie H Meijer
CXC chemokine receptor 4 plays a critical role in chemotaxis and leukocyte differentiation. Furthermore, there is increasing evidence that links this receptor to angiogenesis. Using the well-established zebrafish-Mycobacterium marinum model for tuberculosis, angiogenesis was recently found to be important for the development of cellular aggregates called granulomas that contain the mycobacteria and are the hallmark of tuberculosis disease. Here, we found that initiation of the granuloma-associated proangiogenic programme requires CXCR4 signalling...
March 23, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28325782/aibp-limits-angiogenesis-thorough-%C3%AE-secretase-mediated-upregulation-of-notch-signaling
#2
Renfang Mao, Shu Meng, Qilin Gu, Raquel Araujo-Gutierrez, Sandeep Kumar, Qing Yan, Felicidad Almazan, Keith A Youker, Yingbin Fu, Henry J Pownall, John P Cooke, Yury I Miller, Longhou Fang
Rationale: Angiogenesis improves perfusion to the ischemic tissue following acute vascular obstruction. Angiogenesis in pathophysiological settings reactivates signaling pathways involved in developmental angiogenesis. We showed previously that apoA-I binding protein (AIBP)-regulated cholesterol efflux in endothelial cells (ECs) controls zebrafish embryonic angiogenesis. Objective: This study is to determine whether loss of AIBP affects angiogenesis in mice during development and under pathological conditions, and to explore the underlying molecular mechanism...
March 21, 2017: Circulation Research
https://www.readbyqxmd.com/read/28323620/discovery-of-novel-determinants-of-endothelial-lineage-using-chimeric-heterokaryons
#3
Wing Tak Wong, Gianfranco Matrone, XiaoYu Tian, Simion Alin Tomoiaga, Kin Fai Au, Shu Meng, Sayumi Yamazoe, Daniel Sieveking, Kaifu Chen, David M Burns, James K Chen, Helen M Blau, John P Cooke
We wish to identify determinants of endothelial lineage. Murine embryonic stem cells (mESC) were fused with human endothelial cells in stable, non-dividing, heterokaryons. Using RNA-seq it is possible to discriminate between human and mouse transcripts in these chimeric heterokaryons. We observed a temporal pattern of gene expression in the ESCs of the heterokaryons that recapitulated ontogeny, with early mesodermal factors being expressed before mature endothelial genes. A set of transcriptional factors not known to be involved in endothelial development was upregulated, one of which was POU class 3 homeobox 2 (Pou3f2)...
March 21, 2017: ELife
https://www.readbyqxmd.com/read/28285134/loss-of-gspt1l-disturbs-the-patterning-of-the-brain-central-arteries-in-zebrafish
#4
Hongcheng Wang, Lingfei Luo, Deqin Yang
The cranial vasculature is crucial for the survival and development of the central nervous system and is closely related to brain pathologies. Characterizations of the underlying mechanisms by which cranial vessels acquire their stereotypic patterning remain to be the key interest in the cerebrovascular research. In this report, we show an interesting zebrafish cq37 mutant displaying aberrant patterning of the central arteries. Genetic mapping results indicate that the gene responsible for cq37 encodes G1 to S phase transition 1, like (Gspt1l) with a nonsense mutation...
March 8, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28282489/systematic-functional-testing-of-rare-variants-contributions-of-cfi-to-age-related-macular-degeneration
#5
Perciliz L Tan, Melanie E Garrett, Jason R Willer, Peter A Campochiaro, Betsy Campochiaro, Donald J Zack, Allison E Ashley-Koch, Nicholas Katsanis
Purpose: Genome-wide association (GWAS) and sequencing studies for AMD have highlighted the importance of coding variants at loci that encode components of the complement pathway. However, assessing the contribution of such alleles to AMD, especially when they are rare, remains coarse, in part because of the persistent challenge in establishing their functional relevance. Others and we have shown previously that rare alleles in complement factor I (CFI) can be tested functionally using a surrogate in vivo assay of retinal vascularization in zebrafish embryos...
March 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/28279709/vegf-signaling-promotes-vascular-endothelial-differentiation-by-modulating-etv2-expression
#6
Satish Casie Chetty, Megan S Rost, Jacob Ryan Enriquez, Jennifer A Schumacher, Kristina Baltrunaite, Andrea Rossi, Didier Y R Stainier, Saulius Sumanas
Vasculogenesis involves the differentiation of vascular endothelial progenitors de novo from undifferentiated mesoderm, their migration and coalescence to form the major embryonic vessels and the acquisition of arterial or venous identity. Vascular Endothelial Growth Factor (Vegf) signaling plays multiple roles during vascular development. However, its function during embryonic vasculogenesis has been controversial. Previous studies have implicated Vegf signaling in either regulating arteriovenous specification or overall vascular endothelial differentiation...
March 7, 2017: Developmental Biology
https://www.readbyqxmd.com/read/28271280/rcan1-4-regulates-vegfr-2-internalisation-cell-polarity-and-migration-in-human-microvascular-endothelial-cells
#7
Ahmad F Alghanem, Emma L Wilkinson, Maxine S Emmett, Mohammad A Aljasir, Katherine Holmes, Beverley A Rothermel, Victoria A Simms, Victoria L Heath, Michael J Cross
Regulator of calcineurin 1 (RCAN1) is an endogenous inhibitor of the calcineurin pathway in cells. It is expressed as two isoforms in vertebrates: RCAN1.1 is constitutively expressed in most tissues, whereas transcription of RCAN1.4 is induced by several stimuli that activate the calcineurin-NFAT pathway. RCAN1.4 is highly upregulated in response to VEGF in human endothelial cells in contrast to RCAN1.1 and is essential for efficient endothelial cell migration and tubular morphogenesis. Here, we show that RCAN1...
March 7, 2017: Angiogenesis
https://www.readbyqxmd.com/read/28264837/distinct-and-redundant-functions-of-esam-and-ve-cadherin-during-vascular-morphogenesis
#8
Loïc Sauteur, Markus Affolter, Heinz-Georg Belting
The cardiovascular system forms during early embryogenesis and adapts to embryonic growth by sprouting angiogenesis and vascular remodeling. These processes require fine-tuning of cell-cell adhesion to maintain and reestablish endothelial contacts, while allowing cell motility. We have compared the contribution of two endothelial cell specific adhesion proteins - VE-cadherin (VE-cad/Cdh5) and Esama (Endothelial cell-selective adhesion molecule a) - during angiogenic sprouting and blood vessel fusion (anastomosis) in the zebrafish embryo by genetic analyses...
March 6, 2017: Development
https://www.readbyqxmd.com/read/28230770/vegfa-impacts-early-myocardium-development-in-zebrafish
#9
Diqi Zhu, Yabo Fang, Kun Gao, Jie Shen, Tao P Zhong, Fen Li
Vascular endothelial growth factor A (Vegfa) signaling regulates cardiovascular development. However, the cellular mechanisms of Vegfa signaling in early cardiogenesis remain poorly understood. The present study aimed to understand the differential functions and mechanisms of Vegfa signaling in cardiac development. A loss-of-function approach was utilized to study the effect of Vegfa signaling in cardiogenesis. Both morphants and mutants for vegfaa display defects in cardiac looping and chamber formation, especially the ventricle...
February 21, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28222298/vascular-morphogenesis-an-integrin-and-fibronectin-highway
#10
Hellyeh Hamidi, Johanna Ivaska
A new study shows that endothelial cells use synaptic-like machinery to control polarized secretion and deposition of newly synthesised fibronectin. This process is coupled to active integrin recycling to the same locations and is fundamental for vascular development in zebrafish.
February 20, 2017: Current Biology: CB
https://www.readbyqxmd.com/read/28192181/in-vitro-development-of-zebrafish-vascular-networks
#11
Muhammad Ibrahim, Michael K Richardson
A major limitation to culturing tissues and organs is the lack of a functional vascular network in vitro. The zebrafish possess many useful properties which makes it a promising model for such studies. Unfortunately, methods of culturing endothelial cells from this species are not well characterised. Here, we tried two methods (embryoid body culture and organ explants from transgenic zebrafish kdrl:GFP embryos) to develop in vitro vascular networks. In the kdrl:GFP line, endothelial cells expresses green fluorescent protein, which allows to track the vascular development in live cultures...
February 10, 2017: Reproductive Toxicology
https://www.readbyqxmd.com/read/28192065/vascular-endothelial-growth-factor-a-and-leptin-expression-associated-with-ectopic-proliferation-and-retinal-dysplasia-in-zebrafish-optic-pathway-tumors
#12
Laura E Schultz, Staci L Solin, Wesley A Wierson, Janna M Lovan, Judith Syrkin-Nikolau, Deborah E Lincow, Andrew J Severin, Donald S Sakaguchi, Maura McGrail
In the central nervous system injury induces cellular reprogramming and progenitor proliferation, but the molecular mechanisms that limit regeneration and prevent tumorigenesis are not completely understood. We previously described a zebrafish optic pathway tumor model in which transgenic Tg(flk1:RFP)is18/+ adults develop nonmalignant retinal tumors. Key pathways driving injury-induced glial reprogramming and regeneration contributed to tumor formation. In this study, we examine a time course of proliferation and present new analyses of the Tg(flk1:RFP)is18/+ dysplastic retina and tumor transcriptomes...
February 13, 2017: Zebrafish
https://www.readbyqxmd.com/read/28191018/thymosin-%C3%AE-4-improves-differentiation-and-vascularization-of-ehts
#13
Tilman Ziegler, Rabea Hinkel, Andrea Stöhr, Thomas Eschenhagen, Karl-Ludwig Laugwitz, Ferdinand le Noble, Robert David, Arne Hansen, Christian Kupatt
Induced pluripotent stem cells (iPSC) constitute a powerful tool to study cardiac physiology and represents a promising treatment strategy to tackle cardiac disease. However, iPSCs remain relatively immature after differentiation. Additionally, engineered heart tissue (EHT) has been investigated as a therapy option in preclinical disease models with promising results, although their vascularization and functionality leave room for improvement. Thymosin β4 (Tβ4) has been shown to promote the differentiation of progenitor cell lines to cardiomyocytes while it also induces angiogenic sprouting and vascular maturation...
2017: Stem Cells International
https://www.readbyqxmd.com/read/28182631/zebrafish-atoh8-mutants-do-not-recapitulate-morpholino-phenotypes
#14
Elsie S Place, James C Smith
Atoh8 is a bHLH transcription factor expressed in pancreas, skeletal muscle, the nervous system, and cardiovascular tissues during embryological development. Although it has been implicated in the regulation of pancreatic and endothelial cell differentiation, the phenotypic consequences of Atoh8 loss are uncertain. Conclusions from knockout studies in the mouse differ widely depending on the targeting strategy used, while atoh8 knockdown by interfering morpholino oligonucleotides (morpholinos) in zebrafish has led to a range of developmental defects...
2017: PloS One
https://www.readbyqxmd.com/read/28179430/polydom-is-an-extracellular-matrix-protein-involved-in-lymphatic-vessel-remodeling
#15
Nanami Morooka, Sugiko Futaki, Ryoko Sato-Nishiuchi, Masafumi Nishino, Yuta Totani, Chisei Shimono, Itsuko Nakano, Hiroyuki Nakajima, Naoki Mochizuki, Kiyotoshi Sekiguchi
Rationale: Lymphatic vasculature constitutes a second vascular system, essential for immune surveillance and tissue fluid homeostasis. Maturation of the hierarchical vascular structure, with a highly-branched network of capillaries and ducts, is crucial for its function. Environmental cues mediate the remodeling process, but the mechanism that underlies this process is largely unknown. Objective: Polydom (also called Svep1) is an extracellular matrix protein identified as a high-affinity ligand for integrin α9β1...
February 8, 2017: Circulation Research
https://www.readbyqxmd.com/read/28153846/miconazole-protects-blood-vessels-from-matrix-metalloproteinase-9-dependent-rupture-and-hemorrhage
#16
Ran Yang, Yunpei Zhang, Dandan Huang, Xiao Luo, Liangren Zhang, Xiaojun Zhu, Xiaolin Zhang, Zhenming Liu, Jingyan Han, Jing-Wei Xiong
Hemorrhagic stroke accounts for 10-15% of all strokes and is strongly associated with mortality and morbidity worldwide, but its prevention and therapeutic interventions remain a major challenge. Here, we report the identification of miconazole as a hemorrhagic suppressor by a small-molecule screen in zebrafish. We found that a hypomorphic mutant fn40a, one of known β-pix mutant alleles in zebrafish, had the major symptoms of brain hemorrhage, vessel rupture, and inflammation as those in hemorrhagic stroke patients...
February 2, 2017: Disease Models & Mechanisms
https://www.readbyqxmd.com/read/28153617/the-susceptibility-pathogenesis-of-the-moyamoya-disease
#17
Juntao Hu, Jie Luo, Qianxue Chen
Moyamoya disease (MMD) is a cerebrovascular disease characterized by progressive stenosis of the intracranial internal carotid arteries and their proximal branches. Epidemiologically, MMD is more prevalent in east Asia than any region worldwide, which have been estimated at 0.94 per 100 000 in Japanese and 0.43 per 100,000 in Chinese.However, the etiology of this rare disease remains unknown. Regarding biomarkers of moyamoya disease, moyamoya disease is characterized by an increased expression of angiogenic factors and pro-inflammatory molecules such as vascular endothelial growth factors and matrix metalloproteinase-9, which may partly explain its clinical manifestations of the pathologic angiogenesis, spontaneous hemorrhage, and higher incidence of cerebral hyperperfusion after revascularization surgery...
January 30, 2017: World Neurosurgery
https://www.readbyqxmd.com/read/28148768/variation-in-wall-shear-stress-in-channel-networks-of-zebrafish-models
#18
Woorak Choi, Hye Mi Kim, Sungho Park, Eunseop Yeom, Junsang Doh, Sang Joon Lee
Physiological functions of vascular endothelial cells (ECs) vary depending on wall shear stress (WSS) magnitude, and the functional change affects the pathologies of various cardiovascular systems. Several in vitro and in vivo models have been used to investigate the functions of ECs under different WSS conditions. However, these models have technical limitations in precisely mimicking the physiological environments of ECs and monitoring temporal variations of ECs in detail. Although zebrafish (Danio rerio) has several strategies to overcome these technical limitations, zebrafish cannot be used as a perfect animal model because applying various WSS conditions on blood vessels of zebrafish is difficult...
February 2017: Journal of the Royal Society, Interface
https://www.readbyqxmd.com/read/28137359/pharmacological-targeting-of-the-transcription-factor-sox18-delays-breast-cancer-in-mice
#19
Jeroen Overman, Frank Fontaine, Mehdi Moustaqil, Deepak Mittal, Emma Sierecki, Natalia Sacilotto, Johannes Zuegg, Avril Ab Robertson, Kelly Holmes, Angela A Salim, Sreeman Mamidyala, Mark S Butler, Ashley S Robinson, Emmanuelle Lesieur, Wayne Johnston, Kirill Alexandrov, Brian L Black, Benjamin M Hogan, Sarah De Val, Robert J Capon, Jason S Carroll, Timothy L Bailey, Peter Koopman, Ralf Jauch, Mark J Smyth, Matthew A Cooper, Yann Gambin, Mathias Francois
Pharmacological targeting of transcription factors holds great promise for the development of new therapeutics, but strategies based on blockade of DNA binding, nuclear shuttling, or individual protein partner recruitment have yielded limited success to date. Transcription factors typically engage in complex interaction networks, likely masking the effects of specifically inhibiting single protein-protein interactions. Here, we used a combination of genomic, proteomic and biophysical methods to discover a suite of protein-protein interactions involving the SOX18 transcription factor, a known regulator of vascular development and disease...
January 31, 2017: ELife
https://www.readbyqxmd.com/read/28122229/cilia-control-vascular-mural-cell-recruitment-in-vertebrates
#20
Xiaowen Chen, Dafne Gays, Carlo Milia, Massimo M Santoro
Vascular mural cells (vMCs) are essential components of the vertebrate vascular system, controlling blood vessel maturation and homeostasis. Discrete molecular mechanisms have been associated with vMC development and differentiation. The function of hemodynamic forces in controlling vMC recruitment is unclear. Using transgenic lines marking developing vMCs in zebrafish embryos, we find that vMCs are recruited by arterial-fated vessels and that the process is flow dependent. We take advantage of tissue-specific CRISPR gene targeting to demonstrate that hemodynamic-dependent Notch activation and the ensuing arterial genetic program is driven by endothelial primary cilia...
January 24, 2017: Cell Reports
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