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https://www.readbyqxmd.com/read/29157819/pro-angiogenic-effects-of-ilexsaponin-a1-on-human-umbilical-vein-endothelial-cells-in-vitro-and-zebrafish-in-vivo
#1
Jingjing Li, Jinming Zhang, Liang Zou, Simon Ming-Yuen Lee, Cui Yang, Sai-Wang Seto, George Pak-Heng Leung
BACKGROUND: Ilexsaponin A1 is the major bioactive ingredient of Ilex pubescens Hook. et Arn. This plant has been conventionally used in Traditional Chinese Medicine for the treatment of cardiovascular diseases including stroke, coronary arterial disease, and peripheral vascular diseases. PURPOSE: To investigate the pro-angiogenic effect of Ilexsaponin A1 and its mechanism of action. STUDY DESIGN: Human umbilical vein endothelial cells (HUVECs) and transgenic zebrafish Tg(fli1:EGFP) were employed as an in vitro and in vivo model respectively...
December 1, 2017: Phytomedicine: International Journal of Phytotherapy and Phytopharmacology
https://www.readbyqxmd.com/read/29123087/live-imaging-molecular-changes-in-junctional-tension-upon-ve-cadherin-in-zebrafish
#2
Anne Karine Lagendijk, Guillermo A Gomez, Sungmin Baek, Daniel Hesselson, William E Hughes, Scott Paterson, Daniel E Conway, Heinz-Georg Belting, Markus Affolter, Kelly A Smith, Martin A Schwartz, Alpha S Yap, Benjamin M Hogan
Forces play diverse roles in vascular development, homeostasis and disease. VE-cadherin at endothelial cell-cell junctions links the contractile acto-myosin cytoskeletons of adjacent cells, serving as a tension-transducer. To explore tensile changes across VE-cadherin in live zebrafish, we tailored an optical biosensor approach, originally established in vitro. We validate localization and function of a VE-cadherin tension sensor (TS) in vivo. Changes in tension across VE-cadherin observed using ratio-metric or lifetime FRET measurements reflect acto-myosin contractility within endothelial cells...
November 10, 2017: Nature Communications
https://www.readbyqxmd.com/read/29109778/a-h2o2-responsive-theranostic-probe-for-endothelial-injury-imaging-and-protection
#3
Cheng-Kun Wang, Juan Cheng, Xing-Guang Liang, Chao Tan, Quan Jiang, Yong-Zhou Hu, Ying-Mei Lu, Kohji Fukunaga, Feng Han, Xin Li
Overproduction of H2O2 causes oxidative stress and is the hallmark of vascular diseases. Tracking native H2O2 in the endothelium is therefore indispensable to gain fundamental insights into this pathogenesis. Previous fluorescent probes for H2O2 imaging were generally arylboronates which were decomposed to emissive arylphenols in response to H2O2. Except the issue of specificity challenged by peroxynitrite, boric acid by-produced in this process is actually a waste with unknown biological effects. Therefore, improvements could be envisioned if a therapeutic agent is by-produced instead...
2017: Theranostics
https://www.readbyqxmd.com/read/29037991/vegfa-signaling-regulates-diverse-artery-vein-formation-in-vertebrate-vasculatures
#4
Daqing Jin, Diqi Zhu, Yabo Fang, Yiwei Chen, Gaihong Yu, Weijun Pan, Dong Liu, Fen Li, Tao P Zhong
Vascular endothelial growth factor A (Vegfa) signaling regulates vascular development during embryogenesis and organ formation. However, the signaling mechanisms that govern the formation of various arteries/veins in various tissues are incompletely understood. In this study, we utilized transcription activator-like effector nuclease (TALEN) to generate zebrafish vegfaa mutants. vegfaa(-/-) embryos are embryonic lethal, and display a complete loss of the dorsal aorta (DA) and expansion of the cardinal vein...
September 21, 2017: Journal of Genetics and Genomics, Yi Chuan Xue Bao
https://www.readbyqxmd.com/read/29037123/ultrafine-particle-exposure-reveals-the-importance-of-foxo1-notch-activation-complex-for-vascular-regeneration
#5
Kyung In Baek, René R Sevag Packard, Jeffrey J Hsu, Arian Saffari, Zhao Ma, Anh Phuong Luu, Andrew Pietersen, Hilary Yen, Bin Ren, Yichen Ding, Constantinos Sioutas, Rongsong Li, Tzung K Hsiai
AIMS: Redox active ultrafine particles (UFP, d < 0.2 μm) promote vascular oxidative stress and atherosclerosis. Notch signaling is intimately involved in vascular homeostasis, in which forkhead box O1 (FOXO1) acts as a co-activator of the Notch activation complex. We elucidated the importance of FOXO1/Notch transcriptional activation complex to restore vascular regeneration after UFP exposure. RESULTS: In a zebrafish model of tail injury and repair, transgenic Tg(fli1:GFP) embryos developed vascular regeneration at 3 days post amputation (dpa), whereas UFP exposure impaired regeneration (p < 0...
November 17, 2017: Antioxidants & Redox Signaling
https://www.readbyqxmd.com/read/29017932/tanshinone-i-prevents-atorvastatin-induced-cerebral-hemorrhage-in-zebrafish-and-stabilizes-endothelial-cell-cell-adhesion-by-inhibiting-ve-cadherin-internalization-and-actin-myosin-contractility
#6
Bin Huang, Zhong-Yan Zhou, Shang Li, Xiao-Hui Huang, Jing-Yi Tang, Maggie Pui Man Hoi, Simon Ming Yuen Lee
Defects in vascular integrity in cerebrovasculature lead to serious pathologies including hemorrhagic stroke. The stability of cell adhesion junctions and actin-myosin contractile machinery are two major determinants for the integrity of endothelial monolayer. Here we have evaluated the protective effects of tanshinone I (Tan I), a lipophilic compound presents in Salvia miltiorrhiza, against atorvastatin-induced cerebral hemorrhage in zebrafish in vivo, and further dissected the molecular mechanisms in HUVECs...
October 7, 2017: Pharmacological Research: the Official Journal of the Italian Pharmacological Society
https://www.readbyqxmd.com/read/28994774/establishment-of-larval-zebrafish-as-an-animal-model-to-investigate-trypanosoma-cruzi-motility-in-vivo
#7
Veronica Akle, Nathalie Agudelo-Dueñas, Maria A Molina-Rodriguez, Laurel Brianne Kartchner, Annette Marie Ruth, John M González, Manu Forero-Shelton
Chagas disease is a parasitic infection caused by Trypanosoma cruzi, whose motility is not only important for localization, but also for cellular binding and invasion. Current animal models for the study of T. cruzi allow limited observation of parasites in vivo, representing a challenge for understanding parasite behavior during the initial stages of infection in humans. This protozoan has a flagellar stage in both vector and mammalian hosts, but there are no studies describing its motility in vivo.The objective of this project was to establish a live vertebrate zebrafish model to evaluate T...
September 30, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28950100/how-to-plumb-a-pisces-understanding-vascular-development-and-disease-using-zebrafish-embryos
#8
REVIEW
Benjamin M Hogan, Stefan Schulte-Merker
Our vasculature plays diverse and critical roles in homeostasis and disease. In recent decades, the use of zebrafish has driven our understanding of vascular development into new areas, identifying new genes and mechanisms controlling vessel formation and allowing unprecedented observation of the cellular and molecular events that shape the developing vasculature. Here, we highlight key mechanisms controlling formation of the zebrafish vasculature and investigate how knowledge from this highly tractable model system has informed our understanding of vascular disease in humans...
September 25, 2017: Developmental Cell
https://www.readbyqxmd.com/read/28946938/ldb2-regulates-the-expression-of-dll4-through-the-formation-of-oligomeric-complexes-in-endothelial-cells
#9
Hyun-Jung Choi, Seung-Sik Rho, Dong-Hoon Choi, Young-Guen Kwon
Delta-like ligand 4 (DLL4) expression in endothelial cells is intimately associated with angiogenic sprouting and vascular remodeling, but the precise mechanism of transcriptional regulation of DLL4 remains incompletely understood. Here, we showed that LIM-domain binding protein 2 (LDB2) plays an important role in regulating basal DLL4 and VEGF-induced DLL4 expression. Knockdown of LDB2 using siRNA enhanced endothelial sprouting and tubular network formation in vitro. Injection of ldb2-morpholino resulted in defective development of intersegmental vessels in zebrafish...
September 26, 2017: BMB Reports
https://www.readbyqxmd.com/read/28939876/thrombopoietic-stimulating-activity-of-rhtyrrs-y341a
#10
Yu Jinchao, Zhang Yanling, Wang Xu, Zhao Bing, Ye Yuhao, Zhou Weiran, Sun Shaoyang, Ma Liyun, Shi Yun, Zhan Ling, Yu Min, Mo Wei
Tumor radiotherapy induces hematopoietic organ damage and reduces thrombocyte counts. Thrombocytopenia is a common disease. Some studies have shown that tRNA synthetase plays not only catalytic tRNA aminoacylation roles, but also functions similarly to cytokines. Recombinant human tyrosyl-tRNA synthetase with a mutated Y341A (rhTyrRS (Y341A)) promotes megakaryocyte migrate from bone marrow to peripheral blood. It would promote megakaryocytes in the lungs adhering to vascular endothelial cells and resulting in the platelet production...
September 22, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28938553/homocysteine-induced-peripheral-microcirculation-dysfunction-in-zebrafish-and-its-attenuation-by-l-arginine
#11
Sang Joon Lee, Sung Ho Park, Jinhyuk Fred Chung, Woorak Choi, Hyung Kyu Huh
Elevated blood homocysteine (Hcy) level is frequently observed in aged individuals and those with age-related vascular diseases. However, its effect on peripheral microcirculation is still not fully understood. Using in vivo zebrafish model, the degree of Hcy-induced peripheral microcirculation dysfunction is assessed in this study with a proposed dimensionless velocity parameter [Formula: see text], where [Formula: see text] and [Formula: see text] represent the peripheral microcirculation perfusion and the systemic perfusion levels, respectively...
August 29, 2017: Oncotarget
https://www.readbyqxmd.com/read/28938538/mir-23a-targets-runx2-and-suppresses-ginsenoside-rg1-induced-angiogenesis-in-endothelial-cells
#12
Xiao-Dong Wu, Ting Guo, Li Liu, Chao Wang, Kun Zhang, Han-Qiang Liu, Feng Wang, Wen-Dong Bai, Meng-Yao Zhang
Rg1 is a predominant protopanaxatriol-type of ginsenoside found in Panax ginseng, and it has been shown to have anti-cancer effects in multiple types of cancer cells. However, Rg1 also induces the expression of proangiogenic factors, such as vascular endothelial growth factor (VEGF-A), in endothelial cells. Unfortunately, angiogenesis positively correlates with cancer development. In this study, we identified RUNX2 as a regulator of ginsenoside Rg1-induced angiogenesis for the first time. We found that RUNX2 was directly targeted and regulated by miR-23a...
August 29, 2017: Oncotarget
https://www.readbyqxmd.com/read/28936181/novel-heparin-receptor-transmembrane-protein-184a-regulates-angiogenesis-in-the-adult-zebrafish-caudal-fin
#13
Sara Lynn N Farwell, Kimberly G Reylander, M Kathryn Iovine, Linda J Lowe-Krentz
Transmembrane protein 184A (TMEM184A) was recently identified as the heparin receptor in vascular cells. Heparin binds specifically to TMEM184A and induces anti-proliferative signaling in vitro. Though it is highly conserved, the physiological function of TMEM184A remains unknown. The objective of this study was to investigate the expression and effects on vascular regeneration of TMEM184A using the adult zebrafish regenerating caudal fin as an in vivo model. Here, we show that Tmem184a is expressed in vascular endothelial cells (ECs) of mature and regenerating zebrafish fins...
2017: Frontiers in Physiology
https://www.readbyqxmd.com/read/28934690/butyl-benzyl-phthalate-bbp-induces-caudal-defects-during-embryonic-development
#14
Nicole M Roy, Ewelina Zambrzycka, Jenna Santangelo
Butyl benzyl phthalate (BBP) is commonly added during the manufacturing of plastics to increase flexibility and elasticity. However, BBP leaches off of plastic and environment presence has been detected in soil, groundwater and sediment potentially effecting organisms in the environment. Given the widespread uses of BBP in household, consumer goods and the presence of BBP in the environment, studies on developmental toxicity are needed. Here, we use a zebrafish model to investigate the early developmental toxicity of BBP...
September 18, 2017: Environmental Toxicology and Pharmacology
https://www.readbyqxmd.com/read/28925392/atypical-e2fs-inhibit-tumor-angiogenesis
#15
B G M W Weijts, B Westendorp, B T Hien, L M Martínez-López, M Zijp, I Thurlings, R E Thomas, S Schulte-Merker, W J Bakker, A de Bruin
Atypical E2F transcription factors (E2F7 and E2F8) function as key regulators of cell cycle progression and their inactivation leads to spontaneous cancer formation in mice. However, the mechanism of the tumor suppressor functions of E2F7/8 remain obscure. In this study we discovered that atypical E2Fs control tumor angiogenesis, one of the hallmarks of cancer. We genetically inactivated atypical E2Fs in epithelial and mesenchymal neoplasm and analyzed blood vessel formation in three different animal models of cancer...
September 18, 2017: Oncogene
https://www.readbyqxmd.com/read/28855341/cns-resident-progenitors-direct-the-vascularization-of-neighboring-tissues
#16
Ryota L Matsuoka, Andrea Rossi, Oliver A Stone, Didier Y R Stainier
Organ growth requires the coordinated invasion and expansion of blood vessel networks directed by tissue-resident cells and morphogenetic cues. A striking example of this intercellular communication is the vascularization of the central nervous system (CNS), which is driven by neuronal progenitors, including neuroepithelial cells and radial glia. Although the importance of neuronal progenitors in vascular development within the CNS is well recognized, how these progenitors regulate the vasculature outside the CNS remains largely unknown...
September 19, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28854362/loss-of-apela-peptide-in-mice-causes-low-penetrance-embryonic-lethality-and-defects-in-early-mesodermal-derivatives
#17
Laina Freyer, Chih-Wei Hsu, Sonja Nowotschin, Andrea Pauli, Junji Ishida, Keiji Kuba, Akiyoshi Fukamizu, Alexander F Schier, Pamela A Hoodless, Mary E Dickinson, Anna-Katerina Hadjantonakis
Apela (also known as Elabela, Ende, and Toddler) is a small signaling peptide that activates the G-protein-coupled receptor Aplnr to stimulate cell migration during zebrafish gastrulation. Here, using CRISPR/Cas9 to generate a null, reporter-expressing allele, we study the role of Apela in the developing mouse embryo. We found that loss of Apela results in low-penetrance cardiovascular defects that manifest after the onset of circulation. Three-dimensional micro-computed tomography revealed a higher penetrance of vascular remodeling defects, from which some mutants recover, and identified extraembryonic anomalies as the earliest morphological distinction in Apela mutant embryos...
August 29, 2017: Cell Reports
https://www.readbyqxmd.com/read/28851992/tcf7l2-plays-pleiotropic-roles-in-the-control-of-glucose-homeostasis-pancreas-morphology-vascularization-and-regeneration
#18
Nicola Facchinello, Estefania Tarifeño-Saldivia, Enrico Grisan, Marco Schiavone, Margherita Peron, Alessandro Mongera, Olivier Ek, Nicole Schmitner, Dirk Meyer, Bernard Peers, Natascia Tiso, Francesco Argenton
Type 2 diabetes (T2D) is a disease characterized by impaired insulin secretion. The Wnt signaling transcription factor Tcf7l2 is to date the T2D-associated gene with the largest effect on disease susceptibility. However, the mechanisms by which TCF7L2 variants affect insulin release from β-cells are not yet fully understood. By taking advantage of a tcf7l2 zebrafish mutant line, we first show that these animals are characterized by hyperglycemia and impaired islet development. Moreover, we demonstrate that the zebrafish tcf7l2 gene is highly expressed in the exocrine pancreas, suggesting potential bystander effects on β-cell growth, differentiation and regeneration...
August 29, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28851572/zebrafish-as-an-early-stage-screening-tool-to-study-the-systemic-circulation-of-nanoparticulate-drug-delivery-systems-in-vivo
#19
Sandro Sieber, Philip Grossen, Pascal Detampel, Salome Siegfried, Dominik Witzigmann, Jörg Huwyler
Nanomedicines have gained much attention for the delivery of small molecules or nucleic acids as treatment options for many diseases. However, the transfer from experimental systems to in vivo applications remains a challenge since it is difficult to assess their circulation behavior in the body at an early stage of drug discovery. Thus, innovative and improved concepts are urgently needed to overcome this issue and to close the gap between empiric nanoparticle design, in vitro assessment, and first in vivo experiments using rodent animal models...
October 28, 2017: Journal of Controlled Release: Official Journal of the Controlled Release Society
https://www.readbyqxmd.com/read/28845297/discovery-of-a-rock-inhibitor-fpnd-which-prevents-cerebral-hemorrhage-through-maintaining-vascular-integrity-by-interference-with-ve-cadherin
#20
Shang Li, Nana Ai, Mingyun Shen, Yuanye Dang, Cheong-Meng Chong, Peichen Pan, Yiu Wa Kwan, Shun Wan Chan, George Pak Heng Leung, Maggie Pui Man Hoi, Tingjun Hou, Simon Ming-Yuen Lee
Hemorrhagic stroke occurs when a weakened vessel ruptures and bleeds into the surrounding brain, leading to high rates of death and disability worldwide. A series of complex pathophysiological cascades contribute to the risk of hemorrhagic stroke, and no therapies have proven effective to prevent hemorrhagic stroke. Stabilization of vascular integrity has been considered as a potential therapeutic target for hemorrhagic stroke. ROCKs, which belong to the serine/threonine protein kinase family and participate in the organization of actin cytoskeleton, have become attractive targets for the treatment of strokes...
2017: Cell Death Discovery
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