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https://www.readbyqxmd.com/read/29929848/the-caudal-dorsal-artery-generates-hematopoietic-stem-and-progenitor-cells-via-the-endothelial-to-hematopoietic-transition-in-zebrafish
#1
Yandong Zhan, Youkui Huang, Jingying Chen, Zigang Cao, Jianbo He, Jingjing Zhang, Honghui Huang, Hua Ruan, Lingfei Luo, Li Li
Zebrafish hematopoietic stem and progenitor cells (HSPCs) originate from the hemogenic endothelium of the ventral wall of the dorsal aorta (DA) through the endothelial-to-hematopoietic transition (EHT) from approximately 30 to 60 hours post fertilization (hpf). However, whether other artery sites can generate HSPCs de novo remains unclear. In this study, using live imaging and lineage tracing, we found that the caudal dorsal artery (CDA) in the caudal hematopoietic tissue directly gave rise to HSPCs through EHT...
June 8, 2018: Journal of Genetics and Genomics, Yi Chuan Xue Bao
https://www.readbyqxmd.com/read/29915060/inflamed-neutrophils-sequestered-at-entrapped-tumor-cells-via-chemotactic-confinement-promote-tumor-cell-extravasation
#2
Michelle B Chen, Cynthia Hajal, David C Benjamin, Cathy Yu, Hesham Azizgolshani, Richard O Hynes, Roger D Kamm
Systemic inflammation occurring around the course of tumor progression and treatment are often correlated with adverse oncological outcomes. As such, it is suspected that neutrophils, the first line of defense against infection, may play important roles in linking inflammation and metastatic seeding. To decipher the dynamic roles of inflamed neutrophils during hematogenous dissemination, we employ a multiplexed microfluidic model of the human microvasculature enabling physiologically relevant transport of circulating cells combined with real-time, high spatial resolution observation of heterotypic cell-cell interactions...
June 18, 2018: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/29910999/epigenetic-and-epitranscriptomic-factors-make-a-mark-on-hematopoietic-stem-cell-development
#3
Dionna M Kasper, Stefania Nicoli
Purpose of the Review: Blood specification is a highly dynamic process, whereby committed hemogenic endothelial cells (ECs) progressively transdifferentiate into multipotent, self-renewing hematopoietic stem cells (HSCs). Massive changes in gene expression must occur to switch cell identity, however the factors that mediate such an effect were a mystery until recently. This review summarizes the higher-order mechanisms involved in endothelial to hematopoietic reprogramming identified thus far...
March 2018: Current Stem Cell Reports
https://www.readbyqxmd.com/read/29890255/a-novel-ca-2-current-blocker-promotes-angiogenesis-and-cardiac-healing-after-experimental-myocardial-infarction-in-mice
#4
Guozhen Cui, Qiqi Xin, Hisa Hui Ling Tseng, Maggie PuiMan Hoi, Yan Wang, Binrui Yang, InLeng Choi, Yuqiang Wang, Rong Yuan, Keji Chen, Weihong Cong, Simon MingYuen Lee
We previously reported a novel danshensu derivative (R)-(3,5,6-Trimethylpyrazinyl) methyl-2-acetoxy-3-(3,4-diacetoxyphenyl) propanoate (ADTM) that exhibited promising cardiovascular protective activities, such as antioxidant and antiplatelet activities, as well as arterial relaxation. Particularly, ADTM treatment for 24 h exhibited anti-oxidative activity and effectively protected against acute myocardial infarction (MI) in a rat model. Here, we further investigated the pharmacological actions of 14 days of treatment with ADTM in alleviating and restoring the MI size by stimulating revascularization...
June 8, 2018: Pharmacological Research: the Official Journal of the Italian Pharmacological Society
https://www.readbyqxmd.com/read/29872538/pseudopodium-enriched-atypical-kinase-1-mediates-angiogenesis-by-modulating-gata2-dependent-vegfr2-transcription
#5
Huawei Wang, John Lapek, Ken Fujimura, Jan Strnadel, Bei Liu, David J Gonzalez, Wei Zhang, Felicia Watson, Vicky Yu, Chao Liu, Carina Muccilo Melo, Yury I Miller, Kathryn C Elliott, David A Cheresh, Richard L Klemke
PEAK1 is a newly described tyrosine kinase and scaffold protein that transmits integrin-mediated extracellular matrix (ECM) signals to facilitate cell movement and growth. While aberrant expression of PEAK1 has been linked to cancer progression, its normal physiological role in vertebrate biology is not known. Here we provide evidence that PEAK1 plays a central role in orchestrating new vessel formation in vertebrates. Deletion of the PEAK1 gene in zebrafish, mice, and human endothelial cells (ECs) induced severe defects in new blood vessel formation due to deficiencies in EC proliferation, survival, and migration...
2018: Cell Discovery
https://www.readbyqxmd.com/read/29866703/live-imaging-of-wound-angiogenesis-reveals-macrophage-orchestrated-vessel-sprouting-and-regression
#6
David B Gurevich, Charlotte E Severn, Catherine Twomey, Alexander Greenhough, Jenna Cash, Ashley M Toye, Harry Mellor, Paul Martin
Wound angiogenesis is an integral part of tissue repair and is impaired in many pathologies of healing. Here, we investigate the cellular interactions between innate immune cells and endothelial cells at wounds that drive neoangiogenic sprouting in real time and in vivo Our studies in mouse and zebrafish wounds indicate that macrophages are drawn to wound blood vessels soon after injury and are intimately associated throughout the repair process and that macrophage ablation results in impaired neoangiogenesis...
June 4, 2018: EMBO Journal
https://www.readbyqxmd.com/read/29800868/from-fish-embryos-to-human-patients-lymphangiogenesis-in-development-and-disease
#7
REVIEW
Cristina Mauri, Guangxia Wang, Stefan Schulte-Merker
The lymphatic vasculature plays vital roles in immune surveillance, fluid homeostasis and fat absorption in the body. Lined by endothelial cells, the lymphatic system is functionally distinct from the blood vasculature, and fulfills different physiological functions. In recent years, insight from zebrafish, mice and human patients have improved our understanding of lymphatics, and the interplay between zebrafish genetics, studies in mice and GWAS analysis in human patients have identified genes that, when mutated, will lead to lymphedema formation...
May 22, 2018: Current Opinion in Immunology
https://www.readbyqxmd.com/read/29757409/secretogranin-ii-plays-a-critical-role-in-zebrafish-neurovascular-modeling
#8
Binbin Tao, Hongling Hu, Kimberly Mitchell, Ji Chen, Haibo Jia, Zuoyan Zhu, Vance L Trudeau, Wei Hu
Secretoneurin (SN) is a neuropeptide derived from specific proteolytic processing of the precursor secretogranin II (SgII). In zebrafish and other teleosts, there are two paralogs named sgIIa and sgIIb. Our results showed that neurons expressing sgIIb were aligned with central arteries in the hindbrain, demonstrating a close neurovascular association. Both sgIIb-/- and sgIIa-/-/sgIIb-/- mutant embryos were defective in hindbrain central artery development due to impairment of migration and proliferation of central artery cells...
May 11, 2018: Journal of Molecular Cell Biology
https://www.readbyqxmd.com/read/29752956/advanced-microscopy-to-elucidate-cardiovascular-injury-and-regeneration-4d-light-sheet-imaging
#9
REVIEW
Kyung In Baek, Yichen Ding, Chih-Chiang Chang, Megan Chang, René R Sevag Packard, Jeffrey J Hsu, Peng Fei, Tzung K Hsiai
The advent of 4-dimensional (4D) light-sheet fluorescence microscopy (LSFM) has provided an entry point for rapid image acquisition to uncover real-time cardiovascular structure and function with high axial resolution and minimal photo-bleaching/-toxicity. We hereby review the fundamental principles of our LSFM system to investigate cardiovascular morphogenesis and regeneration after injury. LSFM enables us to reveal the micro-circulation of blood cells in the zebrafish embryo and assess cardiac ventricular remodeling in response to chemotherapy-induced injury using an automated segmentation approach...
May 9, 2018: Progress in Biophysics and Molecular Biology
https://www.readbyqxmd.com/read/29749044/mycobacteria-employ-two-different-mechanisms-to-cross-the-blood-brain-barrier
#10
L M van Leeuwen, M Boot, C Kuijl, D I Picavet, G van den Brink, S M A van der Pol, H E de Vries, N N van der Wel, M van der Kuip, A M van Furth, A M van der Sar, W Bitter
Central nervous system (CNS) infection by Mycobacterium tuberculosis is one of the most devastating complications of tuberculosis, in particular in early childhood. In order to induce CNS infection, M. tuberculosis needs to cross specialized barriers protecting the brain. How M. tuberculosis crosses the blood-brain barrier (BBB) and enters the CNS is not well understood. Here, we use transparent zebrafish larvae and the closely related pathogen Mycobacterium marinum to answer this question. We show that in the early stages of development mycobacteria rapidly infect brain tissue, either as free mycobacteria or within circulating macrophages...
May 10, 2018: Cellular Microbiology
https://www.readbyqxmd.com/read/29744287/a-noncanonical-function-of-histidyl-trna-synthetase-inhibition-of-vascular-hyperbranching-during-zebrafish-development
#11
Rui Ni, Lingfei Luo
Histidyl-tRNA synthetase (Hars) catalyzes the ligation of histidine residues to cognate tRNA. Here, we demonstrate a noncanonical function of Hars in vascular development in zebrafish. We obtained a novel zebrafish cq34 mutant which exhibited hyperbranching of cranial and intersegmental blood vessels 48 h after fertilization. The gene responsible for this phenotype was identified as hars . We found the increased expression of cdh5 and vegfa in the hars cq34 mutant. Knockdown of cdh5 in the mutant reduced disordered connections of the hindbrain capillaries...
May 2018: FEBS Open Bio
https://www.readbyqxmd.com/read/29730311/sodium-tanshinone-iia-sulfonate-promotes-endothelial-integrity-via-regulating-ve-cadherin-dynamics-and-rhoa-rock-mediated-cellular-contractility-and-prevents-atorvastatin-induced-intracerebral-hemorrhage-in-zebrafish
#12
Zhong-Yan Zhou, Bin Huang, Shang Li, Xiao-Hui Huang, Jing-Yi Tang, Yiu Wa Kwan, Pui Man Hoi, Simon Ming-Yuen Lee
Impaired vascular integrity leads to serious cerebral vascular diseases such as intracerebral hemorrhage (ICH). In addition, high-dose statin therapy is suggested to cause increased ICH risk due to unclear effects of general inhibition of 3-hydroxy-3-methylglutaryl-CoA reductase (HMGCR) on the vascular system. Here we evaluated the protective effects of sodium tanshinone IIA sulfonate (STS), which has high efficacy and safety in clinical studies of ischemic stroke, by using atorvastatin (Ator) induced ICH zebrafish embryos and human umbilical vein endothelial cells (HUVECs)...
May 3, 2018: Toxicology and Applied Pharmacology
https://www.readbyqxmd.com/read/29730294/live-imaging-reveals-a-conserved-role-of-fatty-acid-%C3%AE-oxidation-in-early-lymphatic-development-in-zebrafish
#13
Annalisa Zecchin, Brian Wong, Bieke Tembuyser, Joris Souffreau, An Van Nuffelen, Sabine Wyns, Stefan Vinckier, Peter Carmeliet, Mieke Dewerchin
During embryonic development, lymphatic endothelial cells (LECs) differentiate from venous endothelial cells (VECs), a process that is tightly regulated by several genetic signals. While the aquatic zebrafish model is regularly used for studying lymphangiogenesis and offers the unique advantage of time-lapse video-imaging of lymphatic development, some aspects of lymphatic development in this model differ from those in the mouse. It therefore remained to be determined whether fatty acid β-oxidation (FAO), which we showed to regulate lymphatic formation in the mouse, also co-determines lymphatic development in this aquatic model...
May 3, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29724820/temporal-and-spatial-post-transcriptional-regulation-of-zebrafish-tie1-mrna-by-long-noncoding-rna-during-brain-vascular-assembly
#14
Tamjid A Chowdhury, Chris Koceja, Shahram Eisa-Beygi, Benjamin P Kleinstiver, Suresh N Kumar, Chien-Wei Lin, Keguo Li, Shubhangi Prabhudesai, J Keith Joung, Ramani Ramchandran
OBJECTIVE: Tie1 (tyrosine kinase containing immunoglobulin and epidermal growth factor homology 1), an endothelial and hematopoietic cell-specific receptor tyrosine kinase, is an important regulator of angiogenesis and critical for maintaining vascular integrity. The post-transcriptional regulation of tie1 mRNA expression is not understood, but it might partly explain Tie1's differential expression pattern in endothelium. Following up on our previous work that identified natural antisense transcripts from the tie1 locus- tie1 antisense ( tie1AS ), which regulates tie1 mRNA levels in zebrafish-we attempted to identify the mechanism of this regulation...
May 3, 2018: Arteriosclerosis, Thrombosis, and Vascular Biology
https://www.readbyqxmd.com/read/29721065/development-of-a-novel-albumin-based-and-maleimidopropionic-acid-conjugated-peptide-with-prolonged-half-life-and-increased-in-vivo-anti-tumor-efficacy
#15
Junnan Feng, Chuanke Zhao, Lixin Wang, Like Qu, Hua Zhu, Zhi Yang, Guo An, Huifang Tian, Chengchao Shou
Angiogenesis plays a critical role in tumor aggressiveness, and a lot of anti-angiogenic agents have been used in clinical therapy. The therapeutic efficacy of peptides are generally restricted by the short in vivo life-time, thus, we were interested in developing a novel albumin-based and maleimidopropionic acid-conjugated peptide to prolong the half-life and improve the anti-tumor effect. Methods: We developed a peptide F56 with a maleimidopropionic acid (MPA) at the C-terminal (denoted as F56-CM), which allows immediate and irreversible conjugation with serum albumin...
2018: Theranostics
https://www.readbyqxmd.com/read/29712618/lymphatic-endothelium-stimulates-melanoma-metastasis-and-invasion-via-mmp14-dependent-notch3-and-%C3%AE-1-integrin-activation
#16
Pirita Pekkonen, Sanni Alve, Giuseppe Balistreri, Silvia Gramolelli, Olga Tatti-Bugaeva, Ilkka Paatero, Otso Niiranen, Krista Tuohinto, Nina Perälä, Adewale Taiwo, Nadezhda Zinovkina, Pauliina Repo, Katherine Icay, Johanna Ivaska, Pipsa Saharinen, Sampsa Hautaniemi, Kaisa Lehti, Päivi M Ojala
Lymphatic invasion and lymph node metastasis correlate with poor clinical outcome in melanoma. However, the mechanisms of lymphatic dissemination in distant metastasis remain incompletely understood. We show here that exposure of expansively growing human WM852 melanoma cells, but not singly invasive Bowes cells, to lymphatic endothelial cells (LEC) in 3D co-culture facilitates melanoma distant organ metastasis in mice. To dissect the underlying molecular mechanisms, we established LEC co-cultures with different melanoma cells originating from primary tumors or metastases...
May 1, 2018: ELife
https://www.readbyqxmd.com/read/29670362/antiangiogenic-effects-of-aa-pme-on-huvecs-in-vitro-and-zebrafish-in-vivo
#17
Yue Jing, Gang Wang, Qi Xiao, Yachun Zhou, Yingjie Wei, Zhunan Gong
Angiogenesis plays a vital role in many physiological and pathological processes and several diseases are connected with its dysregulation. Asiatic acid (AA) has demonstrated anticancer properties and we suspect this might be attributable to an effect on angio-genesis. A modified derivative of AA, N-(2α,3β,23-acetoxyurs-12-en-28-oyl)-L-proline methyl ester (AA-PMe), has improved efficacy over its parent compound, but its effect on blood vessel development remains unclear. Methods: In this study, we investigated the antiangiogenic activity of AA and AA-PMe in zebrafish embryos and human umbilical vein endothelial cells (HUVECs)...
2018: OncoTargets and Therapy
https://www.readbyqxmd.com/read/29658397/inflammation-coagulation-response-and-thrombotic-effects-induced-by-silica-nanoparticles-in-zebrafish-embryos
#18
Junchao Duan, Shuang Liang, Yang Yu, Yang Li, Lijing Wang, Zehao Wu, Yueyue Chen, Mark R Miller, Zhiwei Sun
Nowadays, nanotechnology environmental health and safety (nanoEHS) is gaining attention. We previously found that silica nanoparticles (SiNPs) could induce vascular endothelial damage. However, the subsequent toxicologic response to SiNPs-induced endothelial damage was still largely unknown. In this study, we explored the inflammation-coagulation response and thrombotic effects of SiNPs in endothelial cells and zebrafish embryos. For in vitro study, swollen mitochondria and autophagosome were observed in ultrastructural analysis...
June 2018: Nanotoxicology
https://www.readbyqxmd.com/read/29655679/the-extract-of-polygoni-cuspidati-rhizoma-et-radix-suppresses-the-vascular-endothelial-growth-factor-induced-angiogenesis
#19
Wei-Hui Hu, Gallant Kar-Lun Chan, Jian-Shu Lou, Qi-Yun Wu, Huai-You Wang, Ran Duan, Michael Yu-Tung Cheng, Tina Ting-Xia Dong, Karl Wah-Keung Tsim
BACKGROUND: Polygoni Cuspidati Rhizoma et Radix (PCRR; the root and rhizome of Polygonum cuspidatum Sieb. et Zucc) is a traditional Chinese medicine for the treatment of inflammation, hyperlipemia, favus, jaundice and scald. HYPOTHESIS/PURPOSE: The extract of PCRR inhibits vascular endothelial growth factor (VEGF)-induced angiogenesis. The hypothesis is supported by analysis of PCRR extract and investigation of pharmacological role and signaling mechanism of PCRR extract in regulating angiogenic responses...
March 15, 2018: Phytomedicine: International Journal of Phytotherapy and Phytopharmacology
https://www.readbyqxmd.com/read/29626786/dynamics-of-dual-fluorescent-polymersomes-with-durable-integrity-in-living-cancer-cells-and-zebrafish-embryos
#20
Sven H C Askes, Nelli Bossert, Jeroen Bussmann, Victorio Saez Talens, Michael S Meijer, Roxanne E Kieltyka, Alexander Kros, Sylvestre Bonnet, Doris Heinrich
The long-term fate of biomedical nanoparticles after endocytosis is often only sparsely addressed in vitro and in vivo, while this is a crucial parameter to conclude on their utility. In this study, dual-fluorescent polyisobutylene-polyethylene glycol (PiB-PEG) polymersomes were studied for several days in vitro and in vivo. In order to optically track the vesicles' integrity, one fluorescent probe was located in the membrane and the other in the aqueous interior compartment. These non-toxic nanovesicles were quickly endocytosed in living A549 lung carcinoma cells but unusually slowly transported to perinuclear lysosomal compartments, where they remained intact and luminescent for at least 90 h without being exocytosed...
June 2018: Biomaterials
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