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https://www.readbyqxmd.com/read/29438257/recent-insights-into-vascular-development-from-studies-in-zebrafish
#1
Ryota L Matsuoka, Didier Y R Stainier
PURPOSE OF REVIEW: Zebrafish has provided a powerful platform to study vascular biology over the past 25 years, owing to their distinct advantages for imaging and genetic manipulation. In this review, we summarize recent progress in vascular biology with particular emphasis on vascular development in zebrafish. RECENT FINDINGS: The advent of transcription activator-like effector nuclease and clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 genome-editing technologies has dramatically facilitated reverse genetic approaches in zebrafish, as in other models...
February 12, 2018: Current Opinion in Hematology
https://www.readbyqxmd.com/read/29414790/histone-acetyltransferase-7-kat7-dependent-intragenic-histone-acetylation-regulates-endothelial-cell-gene-regulation
#2
Matthew S Yan, Paul J Turgeon, Hon Sum Jeffrey Man, Michelle K Dubinsky, Jr Jyun David Ho, Suzan El-Rass, You-Dong Wang, Xiao-Yan Wen, Philip A Marsden
Although the functional role of chromatin marks at promoters in mediating cell-restricted gene expression has been well characterized, the role of intragenic chromatin marks is not well understood, especially in endothelial cell (EC) gene expression. Here, we characterized the histone H3 and H4 acetylation profiles of 19 genes with EC-enriched expression via loci-wide chromatin immunoprecipitation followed by ultra high resolution (5 bp) tiling array analysis in ECs versus non-ECs throughout their genomic loci...
February 6, 2018: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29397482/somatic-nras-mutation-in-patient-with-generalized-lymphatic-anomaly
#3
Eugenia Manevitz-Mendelson, Gil S Leichner, Ortal Barel, Inbal Davidi-Avrahami, Limor Ziv-Strasser, Eran Eyal, Itai Pessach, Uri Rimon, Aviv Barzilai, Abraham Hirshberg, Keren Chechekes, Ninette Amariglio, Gideon Rechavi, Karina Yaniv, Shoshana Greenberger
Generalized lymphatic anomaly (GLA or lymphangiomatosis) is a rare disease characterized by a diffuse proliferation of lymphatic vessels in skin and internal organs. It often leads to progressive respiratory failure and death, but its etiology is unknown. Here, we isolated lymphangiomatosis endothelial cells from GLA tissue. These cells were characterized by high proliferation and survival rates, but displayed impaired capacities for migration and tube formation. We employed whole exome sequencing to search for disease-causing genes and identified a somatic mutation in NRAS...
February 3, 2018: Angiogenesis
https://www.readbyqxmd.com/read/29381707/rasa3-controls-turnover-of-endothelial-cell-adhesion-and-vascular-lumen-integrity-by-a-rap1-dependent-mechanism
#4
Patricia Molina-Ortiz, Tanguy Orban, Maud Martin, Audrey Habets, Franck Dequiedt, Stéphane Schurmans
Rasa3 is a GTPase activating protein of the GAP1 family which targets R-Ras and Rap1. Although catalytic inactivation or deletion of Rasa3 in mice leads to severe hemorrhages and embryonic lethality, the biological function and cellular location of Rasa3 underlying these defects remains unknown. Here, using a combination of loss of function studies in mouse and zebrafish as well as in vitro cell biology approaches, we identify a key role for Rasa3 in endothelial cells and vascular lumen integrity. Specific ablation of Rasa3 in the mouse endothelium, but not in megakaryocytes and platelets, lead to embryonic bleeding and death at mid-gestation, recapitulating the phenotype observed in full Rasa3 knock-out mice...
January 30, 2018: PLoS Genetics
https://www.readbyqxmd.com/read/29364115/heg1-and-ccm1-2-proteins-control-endocardial-mechanosensitivity-during-zebrafish-valvulogenesis
#5
Stefan Donat, Marta Lourenço, Alessio Paolini, Cécile Otten, Marc Renz, Salim Abdelilah-Seyfried
Endothelial cells respond to different levels of fluid shear stress through adaptations of their mechanosensitivity. Currently, we lack a good understanding of how this contributes to sculpting of the cardiovascular system. Cerebral cavernous malformation (CCM) is an inherited vascular disease that occurs when a second somatic mutation causes a loss of CCM1/KRIT1, CCM2, or CCM3 proteins. Here, we demonstrate that zebrafish Krit1 regulates the formation of cardiac valves. Expression of heg1, which encodes a binding partner of Krit1, is positively regulated by blood flow...
January 24, 2018: ELife
https://www.readbyqxmd.com/read/29357127/molecular-regulation-of-sprouting-angiogenesis
#6
Camille L Duran, David W Howell, Jui M Dave, Rebecca L Smith, Melanie E Torrie, Jeffrey J Essner, Kayla J Bayless
The term angiogenesis arose in the 18th century. Several studies over the next 100 years laid the groundwork for initial studies performed by the Folkman laboratory, which were at first met with some opposition. Once overcome, the angiogenesis field has flourished due to studies on tumor angiogenesis and various developmental models that can be genetically manipulated, including mice and zebrafish. In addition, new discoveries have been aided by the ability to isolate primary endothelial cells, which has allowed dissection of various steps within angiogenesis...
December 12, 2017: Comprehensive Physiology
https://www.readbyqxmd.com/read/29339404/hif-1%C3%AE-and-hif-2%C3%AE-regulate-hemogenic-endothelium-and-hematopoietic-stem-cell-formation-in-zebrafish
#7
Claudia Gerri, Michele Marass, Andrea Rossi, Didier Y R Stainier
During development, hematopoietic stem cells (HSCs) derive from specialized endothelial cells (ECs), called hemogenic endothelium (HE), via a process called endothelial-to-hematopoietic transition (EHT). While hypoxia inducible factor-1α (HIF-1α) has been reported to positively modulate EHT in vivo, current data indicate the existence of other regulators of this process. Here we show that in zebrafish Hif-2α also positively modulates HSC formation. Specifically, HSC marker gene expression is strongly decreased in hif-1aa;hif-1ab (hif-1α) and in hif-2aa;hif-2ab (hif-2α) zebrafish mutants and morphants...
January 16, 2018: Blood
https://www.readbyqxmd.com/read/29332242/selective-ikk2-inhibitor-imd0354-disrupts-nf-%C3%AE%C2%BAb-signaling-to-suppress-corneal-inflammation-and-angiogenesis
#8
Anton Lennikov, Pierfrancesco Mirabelli, Anthony Mukwaya, Mira Schaupper, Muthukumar Thangavelu, Mieszko Lachota, Zaheer Ali, Lasse Jensen, Neil Lagali
Corneal neovascularization is a sight-threatening condition caused by angiogenesis in the normally avascular cornea. Neovascularization of the cornea is often associated with an inflammatory response, thus targeting VEGF-A alone yields only a limited efficacy. The NF-κB signaling pathway plays important roles in inflammation and angiogenesis. Here, we study consequences of the inhibition of NF-κB activation through selective blockade of the IKK complex IκB kinase β (IKK2) using the compound IMD0354, focusing on the effects of inflammation and pathological angiogenesis in the cornea...
January 13, 2018: Angiogenesis
https://www.readbyqxmd.com/read/29329397/minar1-is-a-notch2-binding-protein-that-inhibits-angiogenesis-and-breast-cancer-growth
#9
Rachel Xi-Yeen Ho, Rosana D Meyer, Kevin B Chandler, Esma Ersoy, Michael Park, Philip A Bondzie, Nima Rahimi, Huihong Xu, Catherine E Costello, Nader Rahimi
Intrinsically disordered proteins (IDPs)/intrinsically unstructured proteins (IUPs) are characterized by the lack of fixed or stable tertiary structure, and are increasingly recognized as an important class of proteins with major roles in signal transduction and transcriptional regulation. In this study, we report the identification and functional characterization of a previously uncharacterized protein (UPF0258/KIAA1024), major intrinsically disordered Notch2-associated receptor 1 (MINAR1). While MINAR1 carries a single transmembrane domain and a short cytoplasmic domain, it has a large extracellular domain that shares no similarity with known protein sequences...
January 10, 2018: Journal of Molecular Cell Biology
https://www.readbyqxmd.com/read/29329032/targeting-expression-of-adenosine-receptors-during-hypoxia-induced-angiogenesis-a-study-using-zebrafish-model
#10
Navina Panneerselvan, Malathi Ragunathan
Hypoxia is known to be a major player during pathological angiogenesis and adenosine as a negative feedback signaling to maintain oxygen delivery in pathological ischemic condition. We mimicked hypoxic condition and studied angiogenesis by inducing adenosine receptors using forskolin, a plant compound and NECA analogue of adenosine using zebrafish model. Vascular endothelial growth factor (VEGF) is known to play a key role during pathological angiogenesis and regulated by the factors HIF1a under hypoxic condition and recently Notch is proposed to play a negative feedback loop mechanism along with VEGF signaling but the role of adenosine receptor during the process is not known...
January 9, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29320626/directing-nanoparticle-biodistribution-through-evasion-and-exploitation-of-stab2-dependent-nanoparticle-uptake
#11
Frederick Campbell, Frank L Bos, Sandro Sieber, Gabriela Arias-Alpizar, Bjørn E Koch, Jörg Huwyler, Alexander Kros, Jeroen Bussmann
Up to 99% of systemically administered nanoparticles are cleared through the liver. Within the liver, most nanoparticles are thought to be sequestered by macrophages (Kupffer cells), although significant nanoparticle interactions with other hepatic cells have also been observed. To achieve effective cell-specific targeting of drugs through nanoparticle encapsulation, improved mechanistic understanding of nanoparticle-liver interactions is required. Here, we show the caudal vein of the embryonic zebrafish (Danio rerio) can be used as a model for assessing nanoparticle interactions with mammalian liver sinusoidal (or scavenger) endothelial cells (SECs) and macrophages...
January 10, 2018: ACS Nano
https://www.readbyqxmd.com/read/29296234/the-pyrazolyl-urea-gege3-inhibits-tumor-angiogenesis-and-reveals-dystrophia-myotonica-protein-kinase-dmpk-1-as-a-novel-angiogenesis-target
#12
Elda Meta, Beat A Imhof, Patricia Ropraz, Richard J Fish, Chiara Brullo, Olga Bruno, Adama Sidibé
The limitation of targeting VEGF/VEGFR2 signalling to stop angiogenesis in cancer therapy has been blamed on re-activation of alternative receptor tyrosine kinases by compensatory angiogenic factors. Targeting MAPK and PI3K signaling pathways in endothelial cells may be an alternative or complementary approach. Herein we aimed to evaluate the antitumor and antiangiogenic potential of a novel pyrazolyl-urea kinase inhibitor, GeGe3, and to identify its kinase targets. We found GeGe3 to inhibit the proliferation of HUVEC and endothelial tube formation...
December 8, 2017: Oncotarget
https://www.readbyqxmd.com/read/29288822/copper-containing-mesoporous-bioactive-glass-promotes-angiogenesis-in-an-in-vivo-zebrafish-model
#13
Lilian B Romero-Sánchez, Manuel Marí-Beffa, Paloma Carrillo, Miguel Ángel Medina, Aránzazu Díaz-Cuenca
The osteogenic and angiogenic responses of organisms to the ionic products of degradation of bioactive glasses (BGs) are being intensively investigated. The promotion of angiogenesis by copper (Cu) has been known for more than three decades. This element can be incorporated to delivery carriers, such as BGs, and the materials used in biological assays. In this work, Cu-containing mesoporous bioactive glass (MBG) in the SiO2-CaO-P2O5 compositional system was prepared incorporating 5% mol Cu (MBG-5Cu) by replacement of the corresponding amount of Ca...
December 27, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/29284606/growth-differentiation-factor-6-promotes-vascular-stability-by-restraining-vascular-endothelial-growth-factor-signaling
#14
Shlomo Krispin, Amber N Stratman, Chase H Melick, Radu V Stan, Matteo Malinverno, Jamie Gleklen, Daniel Castranova, Elisabetta Dejana, Brant M Weinstein
OBJECTIVE: The assembly of a functional vascular system requires a coordinated and dynamic transition from activation to maturation. High vascular endothelial growth factor activity promotes activation, including junction destabilization and cell motility. Maturation involves junctional stabilization and formation of a functional endothelial barrier. The identity and mechanism of action of prostabilization signals are still mostly unknown. Bone morphogenetic protein receptors and their ligands have important functions during embryonic vessel assembly and maturation...
February 2018: Arteriosclerosis, Thrombosis, and Vascular Biology
https://www.readbyqxmd.com/read/29284110/efficacy-of-lenvatinib-a-multitargeted-tyrosine-kinase-inhibitor-on-laser-induced-cnv-mouse-model-of-neovascular-amd
#15
Xian Wei, Ting Zhang, Yuqin Yao, Shaoxue Zeng, Min Li, Haotian Xiang, Chengjian Zhao, Guiqun Cao, Minhui Li, Ran Wan, Ping Yang, Jinliang Yang
Neovascular age-related macular degeneration (AMD) is a leading cause of vision loss worldwide. Although intravitreal injection of anti-VEGF antibodies and VEGF Trap have significant clinical benefits, the complications of intravitreal injection, drug resistance and patient compliance still need to be concerned. In this study, the effects of an orally administered multi-targeted tyrosine kinase inhibitor (Lenvatinib, E7080) were evaluated in vitro and in vivo on neovascular AMD mouse model. The results showed that E7080 effectively inhibited the proliferation, migration and tubule formation of human choroidal microvascular endothelial cells (HCMECs), and suppressed the angiogenesis of zebrafish subintestinal vessels without causing malformation...
December 25, 2017: Experimental Eye Research
https://www.readbyqxmd.com/read/29243968/chlorogenic-acid-enriched-extract-of-ilex-kudingcha-c-j-tseng-inhibits-angiogenesis-in-zebrafish
#16
Tao Zhong, Linghua Piao, Hyun Jung Kim, Xiande Liu, Shengnan Jiang, Guomin Liu
Kudingcha is a particularly bitter tasting tea that has been widely used in China to eliminate fever and itching eyes, and to clear blood toxins. Kudingcha is considered of value for its potential anticancer effects that are attributed to the presence of characteristic bioactive ingredients. The chlorogenic acid (CGA) derivatives 3-0-caffeoylquinic acid, 5-0-caffeoylquinic acid, 3,5-0-dicaffeoylquinic acid, and 4,5-0-dicaffeoylquinic acid were separated from Ilex kudingcha C.J. Tseng extract by high-performance liquid chromatography (HPLC)-photodiode array detector (PDA) and HPLC-nuclear magnetic resonance (NMR)...
December 2017: Journal of Medicinal Food
https://www.readbyqxmd.com/read/29232705/etv2-mir-130a-jarid2-cascade-regulates-vascular-patterning-during-embryogenesis
#17
Bhairab N Singh, Naoyuki Tahara, Yasuhiko Kawakami, Satyabrata Das, Naoko Koyano-Nakagawa, Wuming Gong, Mary G Garry, Daniel J Garry
Remodeling of the primitive vasculature is necessary for the formation of a complex branched vascular architecture. However, the factors that modulate these processes are incompletely defined. Previously, we defined the role of microRNAs (miRNAs) in endothelial specification. In the present study, we further examined the Etv2-Cre mediated ablation of DicerL/L and characterized the perturbed vascular patterning in the embryo proper and yolk-sac. We mechanistically defined an important role for miR-130a, an Etv2 downstream target, in the mediation of vascular patterning and angiogenesis in vitro and in vivo...
2017: PloS One
https://www.readbyqxmd.com/read/29183943/anisotropic-shear-stress-patterns-predict-the-orientation-of-convergent-tissue-movements-in-the-embryonic-heart
#18
Francesco Boselli, Emily Steed, Jonathan B Freund, Julien Vermot
Myocardial contractility and blood flow provide essential mechanical cues for the morphogenesis of the heart. In general, endothelial cells change their migratory behavior in response to shear stress patterns, according to flow directionality. Here, we assessed the impact of shear stress patterns and flow directionality on the behavior of endocardial cells, the specialized endothelial cells of the heart. At the early stages of zebrafish heart valve formation, we show that endocardial cells are converging to the valve-forming area and that this behavior depends upon mechanical forces...
December 1, 2017: Development
https://www.readbyqxmd.com/read/29168762/dual-roles-of-fer-kinase-are-required-for-proper-hematopoiesis-and-vascular-endothelium-organization-during-zebrafish-development
#19
Emily M Dunn, Elizabeth J Billquist, Amy L VanderStoep, Phillip G Bax, Laura M Westrate, Lisa K McLellan, Shelby C Peterson, Jeffrey P MacKeigan, Aaron P Putzke
Fer kinase, a protein involved in the regulation of cell-cell adhesion and proliferation, has been shown to be required during invertebrate development and has been implicated in leukemia, gastric cancer, and liver cancer. However, in vivo roles for Fer during vertebrate development have remained elusive. In this study, we bridge the gap between the invertebrate and vertebrate realms by showing that Fer kinase is required during zebrafish embryogenesis for normal hematopoiesis and vascular organization with distinct kinase dependent and independent functions...
November 23, 2017: Biology
https://www.readbyqxmd.com/read/29157819/pro-angiogenic-effects-of-ilexsaponin-a1-on-human-umbilical-vein-endothelial-cells-in-vitro-and-zebrafish-in-vivo
#20
Jingjing Li, Jinming Zhang, Liang Zou, Simon Ming-Yuen Lee, Cui Yang, Sai-Wang Seto, George Pak-Heng Leung
BACKGROUND: Ilexsaponin A1 is the major bioactive ingredient of Ilex pubescens Hook. et Arn. This plant has been conventionally used in Traditional Chinese Medicine for the treatment of cardiovascular diseases including stroke, coronary arterial disease, and peripheral vascular diseases. PURPOSE: To investigate the pro-angiogenic effect of Ilexsaponin A1 and its mechanism of action. STUDY DESIGN: Human umbilical vein endothelial cells (HUVECs) and transgenic zebrafish Tg(fli1:EGFP) were employed as an in vitro and in vivo model respectively...
December 1, 2017: Phytomedicine: International Journal of Phytotherapy and Phytopharmacology
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