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https://www.readbyqxmd.com/read/28098802/penduliflaworosin-a-diterpenoid-from-croton-crassifolius-exerts-anti-angiogenic-effect-via-vegf-receptor-2-signaling-pathway
#1
Yeyin Liang, Yubo Zhang, Guocai Wang, Yaolan Li, Weihuan Huang
Anti-angiogenesis targeting vascular endothelial growth factor receptor-2 (VEGFR-2) has been considered as an important strategy for cancer therapy. Penduliflaworosin is a diterpenoid isolated from the plant Croton crassifolius. Our previous study showed that this diterpenoid possesses strong anti-angiogenic activity by inhibiting vessel formation in zebrafish. This study was conducted to further investigate the anti-angiogenic activity and mechanism of penduliflaworosin. Results revealed that penduliflaworosin significantly inhibited VEGF-induced angiogenesis processes including proliferation, invasion, migration, and tube formation of human umbilical vein endothelial cells (HUVECs)...
January 13, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28098794/ftr82-is-critical-for-vascular-patterning-during-zebrafish-development
#2
Hsueh-Wei Chang, Wen-Der Wang, Chien-Chih Chiu, Chiou-Hua Chen, Yi-Shan Wang, Zih-Ying Chen, Wangta Liu, Ming-Hong Tai, Zhi-Hong Wen, Chang-Yi Wu
Cellular components and signaling pathways are required for the proper growth of blood vessels. Here, we report for the first time that a teleost-specific gene ftr82 (finTRIM family, member 82) plays a critical role in vasculature during zebrafish development. To date, there has been no description of tripartite motif proteins (TRIM) in vascular development, and the role of ftr82 is unknown. In this study, we found that ftr82 mRNA is expressed during the development of vessels, and loss of ftr82 by morpholino (MO) knockdown impairs the growth of intersegmental vessels (ISV) and caudal vein plexus (CVP), suggesting that ftr82 plays a critical role in promoting ISV and CVP growth...
January 13, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28098292/an-andrographolide-derivative-agp-26b-exhibiting-anti-angiogenic-activity-in-huvecs-and-zebrafish-via-blocking-the-vegfa-vegfr2-signaling-pathway
#3
Bin Huang, Yuran Peng, Jingjing Li, Shang Li, Yicheng Sun, Decai Wang, Binrui Yang, Judy Yuet-Wa Chan, Huidong Yu, George Pak-Heng Leung, Maggie Pui-Man Hoi, Guo-Chun Zhou, Simon Ming-Yuen Lee
The aim of this study is to investigate the anti-angiogenic properties of andrographolide derivatives AGP-26a (12β-isomer), AGP-26b (12α-isomer) and AGP-26 (4 : 1 mixture of AGP-26a and AGP-26b) in vitro and in vivo. Human umbilical vein endothelial cells (HUVECs) and the Tg(fli-1a:EGFP)y1 zebrafish model were used to identify the anti-angiogenic activities of AGP-26, AGP-26a, and AGP-26b. The results showed that AGP-26b exhibits the strongest inhibitory effect on VEGF-induced proliferation, migration, invasion and formation of capillary-like structures in HUVECs...
January 18, 2017: Molecular BioSystems
https://www.readbyqxmd.com/read/28092764/common-deregulated-gene-expression-profiles-and-morphological-changes-in-developing-zebrafish-larvae-exposed-to-environmental-relevant-high-to-low-concentrations-of-glucocorticoids
#4
Qiyu Chen, Caixia Li, Zhiyuan Gong, Eric Chun Yong Chan, Shane A Snyder, Siew Hong Lam
Synthetic glucocorticoids have been detected in environmental waters and their biological potency have raised concerns of their impact on aquatic vertebrates especially fish. In this study, developing zebrafish larvae exposed to representative glucocorticoids (dexamethasone, prednisolone and triamcinolone) at 50 pM to 50 nM from 3 h post-fertilisation to 5 days post-fertilisation were investigated. Microarray analysis identified 1255, 1531, and 2380 gene probes, which correspondingly mapped to 660, 882 and 1238 human/rodent homologs, as deregulated by dexamethasone, prednisolone and triamcinolone, respectively...
January 6, 2017: Chemosphere
https://www.readbyqxmd.com/read/28087639/vegfd-modulates-both-angiogenesis-and-lymphangiogenesis-during-zebrafish-embryonic-development
#5
Neil I Bower, Adam J Vogrin, Ludovic Le Guen, Huijun Chen, Steven A Stacker, Marc G Achen, Benjamin M Hogan
Vascular endothelial growth factors (VEGFs) control angiogenesis and lymphangiogenesis during development and in pathological conditions. In the zebrafish trunk, Vegfa controls the formation of intersegmental arteries by primary angiogenesis and Vegfc is essential for secondary angiogenesis, the formation of veins and lymphatics. VEGFD has been largely thought of as dispensable for vascular development in vertebrates. Here, we generated a zebrafish vegfd mutant by genome editing. vegfd mutants display significant defects in facial lymphangiogenesis independent of vegfc function...
January 13, 2017: Development
https://www.readbyqxmd.com/read/28071661/neuronal-sflt1-and-vegfaa-determine-venous-sprouting-and-spinal-cord-vascularization
#6
Raphael Wild, Alina Klems, Masanari Takamiya, Yuya Hayashi, Uwe Strähle, Koji Ando, Naoki Mochizuki, Andreas van Impel, Stefan Schulte-Merker, Janna Krueger, Laetitia Preau, Ferdinand le Noble
Formation of organ-specific vasculatures requires cross-talk between developing tissue and specialized endothelial cells. Here we show how developing zebrafish spinal cord neurons coordinate vessel growth through balancing of neuron-derived Vegfaa, with neuronal sFlt1 restricting Vegfaa-Kdrl mediated angiogenesis at the neurovascular interface. Neuron-specific loss of flt1 or increased neuronal vegfaa expression promotes angiogenesis and peri-neural tube vascular network formation. Combining loss of neuronal flt1 with gain of vegfaa promotes sprout invasion into the neural tube...
January 10, 2017: Nature Communications
https://www.readbyqxmd.com/read/28061458/oncometabolite-succinate-promotes-angiogenesis-by-upregulating-vegf-expression-through-gpr91-mediated-stat3-and-erk-activation
#7
Xianmin Mu, Ting Zhao, Che Xu, Wei Shi, Biao Geng, Jiajia Shen, Chen Zhang, Jinshun Pan, Jing Yang, Shi Hu, Yuanfang Lv, Hao Wen, Qiang You
Altered cellular metabolism is now generally acknowledged as a hallmark of cancer cells, the resultant abnormal oncometabolites cause both metabolic and nonmetabolic dysregulation and potential transformation to malignancy. A subset of cancers have been found to be associated with mutations in succinate dehydrogenase genes which result in the accumulation of succinate. However, the function of succinate in tumorigenesis remains unclear. In the present study, we aim to investigate the role of oncometabolite succinate in tumor angiogenesis...
January 4, 2017: Oncotarget
https://www.readbyqxmd.com/read/28061455/enhancement-of-the-bioavailability-of-a-novel-anticancer-compound-acetyltanshinone-iia-by-encapsulation-within-mpeg-plga-nanoparticles-a-study-of-formulation-optimization-toxicity-and-pharmacokinetics
#8
Qi Wang, Na Wei, Xiaofeng Liu, Alex Chang, Kathy Qian Luo
The Poly (ethylene glycol) methyl ether-block-poly (lactide-co-glycolide) (mPEG-PLGA) nanoparticles carrying acetyltanshinone IIA (ATA), a novel anti-breast cancer agent, were prepared by ultrasonic emulsion method to enhance the bioavailability and reduce the toxicity. Systematic optimization of encapsulation process was achieved using an orthogonal design. Drug efficacy analysis showed that ATA nanoparticles were as effective as free ATA against estrogen receptor positive breast cancer cells, but much less toxic towards human endothelial cells...
January 4, 2017: Oncotarget
https://www.readbyqxmd.com/read/28060344/visualizing-the-interrenal-steroidogenic-tissue-and-its-vascular-microenvironment-in-zebrafish
#9
Chih-Wei Chou, Jamie Lin, Hsin-Yu Hou, Yi-Wen Liu
This protocol introduces how to detect differentiated interrenal steroidogenic cells through a simple whole-mount enzymatic activity assay. Identifying differentiated steroidogenic tissues through chromogenic histochemical staining of 3-β-Hydroxysteroid dehydrogenase /Δ(5-4) isomerase (3β-Hsd) activity-positive cells is critical for monitoring the morphology and differentiation of adrenocortical and interrenal tissues in mammals and teleosts, respectively. In the zebrafish model, the optical transparency and tissue permeability of the developing embryos and larvae allow for whole-mount staining of 3β-Hsd activity...
December 21, 2016: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28035003/a-quininib-analogue-and-cysteinyl-leukotreine-receptor-antagonist-inhibits-vegf-independent-angiogenesis-and-exerts-an-additive-anti-angiogenic-response-with-bevacizumab
#10
Clare T Butler, Alison L Reynolds, Miriam Tosetto, Eugene T Dillon, Patrick Guiry, Gerard Cagney, Jacintha O'Sullivan, Breand Aacuten N Kennedy
Excess blood vessel growth contributes to the pathology of metastatic cancers and age-related retinopathies. Despite development of improved treatments, these conditions are associated with high economic costs and drug resistance. Bevacizumab (Avastin®), a monoclonal antibody against vascular endothelial growth factor (VEGF) is used clinically to treat certain types of metastatic cancers. Unfortunately, many patients do not respond or inevitably become resistant to bevacizumab, highlighting the need for more effective anti-angiogenic drugs with novel mechanisms of action...
December 29, 2016: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28007601/c1qr-and-c1qrl-redundantly-regulate-angiogenesis-in-zebrafish-through-controlling-endothelial-cdh5
#11
Jiang Du, Qifen Yang, Lingfei Luo, Deqin Yang
Angiogenesis plays central role in the formation of functional circulation system. Characterizations of the involved factors and signaling pathways remain to be the key interest in the angiogenesis research. In this report, we showed that c1qr/cd93 and c1qrl/clec14a are specifically expressed in the vascular endothelial cells during zebrafish development. Single mutation of c1qr or c1qrl is associated with slightly malformation of inter-segmental vessels (ISVs), whereas double mutant exhibits severe defects in the ISVs formation without affecting early vasculogenesis...
December 19, 2016: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28003365/impairment-of-cargo-transportation-due-to-gbf1-mutation-disrupts-vascular-integrity-and-causes-hemorrhage-in-zebrafish-embryos
#12
Jing Chen, Xiaotong Wu, Likun Yao, Lu Yan, Lin Zhang, Juhui Qiu, Xingfeng Liu, Shunji Jia, Anming Meng
ADP-ribosylation factor (ARF) GTPases are activated by guanine nucleotide exchange factors including Golgi Brefeldin A-resistant factor 1 (Gbf1) and play important roles in regulating organelle structure and cargo-selective vesicle trafficking. However, the developmental role of Gbf1 in vertebrates remains elusive. In this study, we report the zebrafish mutant line tsu3994 that arises from ENU-mediated mutagenesis and is characterized by prominent intracerebral and trunk hemorrhage. The mutant embryos develop hemorrhage accompanied by fewer pigments and shorter caudal fin at day 2 of development...
December 21, 2016: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/27993727/quantum-dot-interactions-and-flow-effects-in-angiogenic-zebrafish-danio-rerio-vessels-and-human-endothelial-cells
#13
Xiao-Yu Jiang, Christopher D Sarsons, M Juliana Gomez-Garcia, David T Cramb, Kristina D Rinker, Sarah J Childs
Nanoparticle (NP) interactions with biological tissues are affected by the size, shape and surface chemistry of the NPs. Here we use in vivo (zebrafish) and in vitro (HUVEC) models to investigate association of quantum dots (QDs) with endothelial cells and the effect of fluid flow. After injection into the developing zebrafish, circulating QDs associate with endothelium and penetrate surrounding tissue parenchyma over time. Amino-functionalized QDs cluster, interact with cells, and clear more rapidly than carboxy-functionalized QDs in vivo, highlighting charge influences...
December 18, 2016: Nanomedicine: Nanotechnology, Biology, and Medicine
https://www.readbyqxmd.com/read/27989880/spatholobi-caulis-extracts-promote-angiogenesis-in-huvecs-in-vitro-and-in-zebrafish-embryos-in-vivo-via-up-regulation-of-vegfrs
#14
Zhong-Yan Zhou, Li-Yun Huan, Wai-Rong Zhao, Nuo Tang, Yu Jin, Jing-Yi Tang
ETHNOPHARMACOLOGICAL RELEVANCE: Spatholobi Caulis is a traditional blood-activating and stasis-dispelling herb medicine, which has been used to treat diseases related to blood stasis syndrome (BSS) by inhibiting platelet aggregation, stimulate hematopoiesis, etc. It has been demonstrated that pro-angiogenesis could improve BSS. However, the pro-angiogenic activity of Spatholobi Caulis was not well elucidated. AIM OF STUDY: To determine the potential pro-angiogenic activity of Spatholobi Caulis and elucidate its underlying mechanism...
October 27, 2016: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/27989824/rapid-and-cost-effective-xenograft-hepatocellular-carcinoma-model-in-zebrafish-for-drug-testing
#15
Federica Tonon, Cristina Zennaro, Barbara Dapas, Michele Carraro, Massimo Mariotti, Gabriele Grassi
We developed a novel, rapid and cost-effective Zebrafish xenograft model of hepatocellular carcinoma (HCC) for drug screening in the disease. Following injection into the yolk sack of Zebrafish larvae of the human HCC cell line JHH6 stained by a vital dye, tumor mass growth was followed by fluorescence microscopy and by human Ki67 quantification. Tumor induced neo-angiogenesis was evaluated by alkaline phosphatase staining of the vessels, by using the Tg(fli1:EGFP)y(1) strain of Zebrafish and by the quantification of the zebrafish vascular endothelial growth factor and of its receptor...
October 29, 2016: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/27979830/molecular-insights-into-adgra2-gpr124-and-reck-intracellular-trafficking
#16
Naguissa Bostaille, Anne Gauquier, Laure Twyffels, Benoit Vanhollebeke
Adgra2, formerly known as Gpr124, is a key regulator of cerebrovascular development in vertebrates. Together with the GPI-anchored glycoprotein Reck, this adhesion GPCR (aGPCR) stimulates Wnt7-dependent Wnt/β-catenin signaling to promote brain vascular invasion in an endothelial cell-autonomous manner. Adgra2 and Reck have been proposed to assemble a receptor complex at the plasma membrane, but the molecular modalities of their functional synergy remain to be investigated. In particular, as typically found in aGPCRs, the ectodomain of Adgra2 is rich in protein-protein interaction motifs whose contributions to receptor function are unknown...
December 15, 2016: Biology Open
https://www.readbyqxmd.com/read/27965017/structure-and-functionalities-of-the-human-c-reactive-protein-compared-to-the-zebrafish-multigene-family-of-c-reactive-like-proteins
#17
REVIEW
Melissa Bello-Perez, Alberto Falco, Regla Medina, Jose Antonio Encinar, Beatriz Novoa, Luis Perez, Amparo Estepa, Julio Coll
Because of the recent discovery of multiple c-reactive protein (crp)-like genes in zebrafish (Danio rerio) with predicted heterogeneous phospholipid-binding amino acid sequences and heterogeneous transcript expression levels in viral survivors and adaptive-deficient mutants, zebrafish constitute an attractive new model for exploring the evolution of these protein's functions, including their possible participation in fish trained immunity. Circulating human CRP belongs to the short pentraxin family of oligomeric proteins that are characteristic of early acute-phase innate responses and is widely used as a clinical inflammation marker...
December 10, 2016: Developmental and Comparative Immunology
https://www.readbyqxmd.com/read/27941332/overexpression-of-tgf-%C3%AE-inducible-microrna-143-in-zebrafish-leads-to-impairment-of-the-glomerular-filtration-barrier-by-targeting-proteoglycans
#18
Janina Müller-Deile, Finn Gellrich, Heiko Schenk, Patricia Schroder, Jenny Nyström, Johan Lorenzen, Hermann Haller, Mario Schiffer
BACKGROUND: TGF-β is known as an important stress factor of podocytes in glomerular diseases. Apart from activation of direct pro-apoptotic pathways we wanted to analyze micro-RNA (miRs) driven regulation of components involved in the integrity of the glomerular filtration barrier induced by TGF-β. Since miR-143-3p (miR-143) is described as a TGF-β inducible miR in other cell types, we examined this specific miR and its ability to induce glomerular pathology. METHODS: We analyzed miR-143 expression in cultured human podocytes after stimulation with TGF-β...
2016: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/27917158/a-zebrafish-model-for-chlamydia-infection-with-the-obligate-intracellular-pathogen-waddlia-chondrophila
#19
Alexander G J Fehr, Maja Ruetten, Helena M B Seth-Smith, Lisbeth Nufer, Andrea Voegtlin, Angelika Lehner, Gilbert Greub, Philip S Crosier, Stephan C F Neuhauss, Lloyd Vaughan
Obligate intracellular chlamydial bacteria of the Planctomycetes-Verrucomicrobia-Chlamydiae (PVC) superphylum are important pathogens of terrestrial and marine vertebrates, yet many features of their pathogenesis and host specificity are still unknown. This is particularly true for families such as the Waddliacea which, in addition to epithelia, cellular targets for nearly all Chlamydia, can infect and replicate in macrophages, an important arm of the innate immune system or in their free-living amoebal counterparts...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27916465/hypoxia-inhibits-lymphatic-thoracic-duct-formation-in-zebrafish
#20
Isabelle Ernens, Andrew I Lumley, Lu Zhang, Yvan Devaux, Daniel R Wagner
Hypoxia promotes blood vessel growth through up-regulation of pro-angiogenic pathways but its role on the lymphatic system remains unclear. The homeobox transcription factor Prox1 is a master control gene for generating lymphatic endothelial cells (LECs) and is up-regulated by hypoxia-inducible factors in mammals. While vascular endothelial growth factor A (VEGFA) is critical for angiogenesis, VEGFC and its receptor VEGF receptor-3 (VEGFR-3) are essential for the initial sprouting and directed migration as well as for the subsequent survival of LECs...
January 22, 2017: Biochemical and Biophysical Research Communications
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