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Lipofuscin

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https://www.readbyqxmd.com/read/28319893/role-of-macrophages-in-age-related-oxidative-stress-and-lipofuscin-accumulation-in-mice
#1
Carmen Vida, Irene Martínez de Toda, Julia Cruces, Antonio Garrido, Mónica Gonzalez-Sanchez, Mónica De la Fuente
The age-related changes in the immune functions (immunosenescence) may be mediated by an increase of oxidative stress and damage affecting leukocytes. Although the "oxidation-inflammation" theory of aging proposes that phagocytes are the main immune cells contributing to "oxi-inflamm-aging", this idea has not been corroborated. The aim of this work was to characterize the age-related changes in several parameters of oxidative stress and immune function, as well as in lipofuscin accumulation ("a hallmark of aging"), in both total peritoneal leukocyte population and isolated peritoneal macrophages...
March 9, 2017: Redox Biology
https://www.readbyqxmd.com/read/28301413/ophthalmic-findings-in-late-stage-sjogren-larsson-syndrome
#2
Tavish Nanda, Jaclyn L Kovach
PURPOSE: To report spectral domain optical coherence tomography and fundus autofluorescence documentation of late stage macular findings associated with Sjogren-Larsson Syndrome in three adult siblings. METHODS: Three adult siblings with Sjogren-Larsson Syndrome underwent ophthalmic examination and imaging. RESULTS: Crystalline maculopathy and subretinal deposits, presumably lipofuscin accumulation, with macular atrophy were present in varying degrees in all three adult siblings...
March 15, 2017: Retinal Cases & Brief Reports
https://www.readbyqxmd.com/read/28299497/multimodal-imaging-in-a-case-of-butterfly-pattern-dystrophy-of-retinal-pigment-epithelium
#3
Vinod Kumar, Devesh Kumawat
AIMS: To report multi-modal imaging findings in a case of butterfly pattern dystrophy of retinal pigment epithelium. METHODS: A middle-aged female with butterfly pattern dystrophy, who presented with progressive loss of vision, was examined using coloured fundus photographs, short wave autofluorescence, swept source optical coherence tomography (SS-OCT), fundus fluorescein angiography and enface optical coherence tomography. RESULTS: Multi-modal imaging was useful in the characterization of the various disease features...
March 15, 2017: International Ophthalmology
https://www.readbyqxmd.com/read/28298893/bis-retinoid-a2e-induces-an-increase-of-basic-fibroblast-growth-factor-via-inhibition-of-extracellular-signal-regulated-kinases-1-2-pathway-in-retinal-pigment-epithelium-cells-and-facilitates-phagocytosis
#4
Delphine Balmer, Linda Bapst-Wicht, Aswin Pyakurel, Martine Emery, Natacha Nanchen, Christian G Bochet, Raphael Roduit
Age-related macular degeneration (ARMD) is the leading cause of vision loss in developed countries. Hallmarks of the disease are well known; indeed, this pathology is characterized by lipofuscin accumulation, is principally composed of lipid-containing residues of lysosomal digestion. The N-retinyl-N-retinylidene ethanolamine (A2E) retinoid which is thought to be a cytotoxic component for RPE is the best-characterized component of lipofuscin so far. Even if no direct correlation between A2E spatial distribution and lipofuscin fluorescence has been established in aged human RPE, modified forms or metabolites of A2E could be involved in ARMD pathology...
2017: Frontiers in Aging Neuroscience
https://www.readbyqxmd.com/read/28291154/assessment-of-drusen-and-other-retinal-degenerative-changes-in-patients-with-hereditary-hemochromatosis
#5
Moreno Menghini, Christian Prünte, Pierre A Krayenbuehl, Albina Nowak
PURPOSE: Iron can exert oxidative damage, and increased accumulation is believed to play a role in age-related macular degeneration. Hereditary hemochromatosis leads to an increase in total body iron. Patients with HH were assessed for drusen and other retinal changes. METHODS: Descriptive uncontrolled study of spectral-domain optical coherence tomography, short-wavelength autofluorescence, and color fundus images from patients with HH were used. Diagnosis of HH was established by measuring ferritin and transferrin saturation, and confirmed by genetic testing...
March 13, 2017: Retina
https://www.readbyqxmd.com/read/28282423/age-related-macular-degeneration-phenotypes-are-associated-with-increased-tumor-necrosis-alpha-and-subretinal-immune-cells-in-aged-cxcr5-knockout-mice
#6
Hu Huang, Ying Liu, Lei Wang, Wen Li
The role of chemokine receptor in age-related macular degeneration (AMD) remains elusive. The objective of this study is to investigate the role of chemokine receptor Cxcr5 in the pathogenesis of AMD. Cxcr5 gene expression levels (mRNA and protein) are higher in the retina and retinal pigment epithelium (RPE) of aged C57BL/6 wild type mice than younger ones. Vascular and glial cells express Cxcr5 and its ligand Cxcl13 in mouse retina. Aged Cxcr5 knockout (-/-) mice develop both early and late AMD-like pathological features...
2017: PloS One
https://www.readbyqxmd.com/read/28267565/automating-cell-detection-and-classification-in-human-brain-fluorescent-microscopy-images-using-dictionary-learning-and-sparse-coding
#7
Maryana Alegro, Panagiotis Theofilas, Austin Nguy, Patricia A Castruita, William Seeley, Helmut Heinsen, Daniela M Ushizima, Lea T Grinberg
BACKGROUND: Immunofluorescence (IF) plays a major role in quantifying protein expression in situ and understanding cell function. It is widely applied in assessing disease mechanisms and in drug discovery research. Automation of IF analysis can transform studies using experimental cell models. However, IF analysis of postmortem human tissue relies mostly on manual interaction, often subjected to low-throughput and prone to error, leading to low inter and intra-observer reproducibility...
March 4, 2017: Journal of Neuroscience Methods
https://www.readbyqxmd.com/read/28260365/-ultrastructural-alteration-of-extraocular-muscle-proprioceptor-in-congenital-idiopathic-nystagmus
#8
C Y Zhang, D Y Bai, Y H Cui, S L Hu, Y Zhang, C Li, L Li
Objective: To explore the ultrastructural alteration of extraocular muscle proprioceptor in congenital idiopathic nystagmus (CIN). Methods: Case-control study. Ten extraocular muscle samples were collected from five CIN children who underwent nystagmus surgeries in Beijing Children's Hospital from March 2015 to March 2016. Another ten extraocular muscle specimens were collected from five strabismus children in surgery at the same period as normal contrast. There were 3 male patients and 2 female patients of CIN with age of 61-147 months (median age: 91 months)...
February 11, 2017: [Zhonghua Yan Ke za Zhi] Chinese Journal of Ophthalmology
https://www.readbyqxmd.com/read/28244572/anti-aging-effect-of-riboflavin-via-endogenous-antioxidant-in-fruit-fly-drosophila-melanogaster
#9
Y-X Zou, M-H Ruan, J Luan, X Feng, S Chen, Z-Y Chu
OBJECTIVES: This study investigated the effect of riboflavin on aging in Drosophila melanogaster (fruit fly). DESIGN: Experimental study. SETTING: Naval Medical Research Institute. PARTICIPANTS: Fruit fly Drosophila melanogaster. INTERVENTION: After lifelong supplement of riboflavin, the lifespan and the reproduction of fruit flies were observed. Hydrogen peroxide (H2O2) was used to mimic oxidative stress damage to fruit flies and the survival time was recorded...
2017: Journal of Nutrition, Health & Aging
https://www.readbyqxmd.com/read/28226256/endocrine-and-cellular-stress-effects-of-zinc-oxide-nanoparticles-and-nifedipine-in-marsh-frogs-pelophylax-ridibundus
#10
Halina Falfushynska, Lesya Gnatyshyna, Oksana Horyn, Inna Sokolova, Oksana Stoliar
Freshwater organisms including amphibians experience increasing exposures to emerging pollutants such as nanoparticles and pharmaceuticals, which can affect their fitness and performance. We studied the effects of two common pollutants extensively used in industry, pharmaceutical and personal care products, nano-zinc oxide (nZnO) and a Ca-channel blocker nifedipine (Nfd), on endocrine status and cellular stress markers of the marsh frog Pelophylax ridibundus. Males were exposed for 14days to nZnO (3.1μM), Zn(2+) (3...
April 2017: Aquatic Toxicology
https://www.readbyqxmd.com/read/28219732/all-trans-retinal-levels-and-formation-of-lipofuscin-precursors-after-bleaching-in-rod-photoreceptors-from-wild-type-and-abca4-mice
#11
Leopold Adler, Chunhe Chen, Yiannis Koutalos
The accumulation of lipofuscin in the cells of the retinal pigment epithelium (RPE) is thought to play an important role in the development and progression of degenerative diseases of the retina. The bulk of RPE lipofuscin originates in reactions of the rhodopsin chromophore, retinal, with components of the photoreceptor outer segment. The 11-cis retinal isomer is generated in the RPE and supplied to rod photoreceptor outer segments where it is incorporated as the chromophore of rhodopsin. It is photoisomerized during light detection to all-trans and subsequently released by photoactivated rhodopsin as all-trans retinal, which is removed through reduction to all-trans retinol in a reaction requiring metabolic input in the form of NADPH...
February 17, 2017: Experimental Eye Research
https://www.readbyqxmd.com/read/28202390/systemic-injection-of-rpe65-programmed-bone-marrow-derived-cells-prevents-progression-of-chronic-retinal-degeneration
#12
Xiaoping Qi, S Louise Pay, Yuanqing Yan, James Thomas, Alfred S Lewin, Lung-Ji Chang, Maria B Grant, Michael E Boulton
Bone marrow stem and progenitor cells can differentiate into a range of non-hematopoietic cell types, including retinal pigment epithelium (RPE)-like cells. In this study, we programmed bone marrow-derived cells (BMDCs) ex vivo by inserting a stable RPE65 transgene using a lentiviral vector. We tested the efficacy of systemically administered RPE65-programmed BMDCs to prevent visual loss in the superoxide dismutase 2 knockdown (Sod2 KD) mouse model of age-related macular degeneration. Here, we present evidence that these RPE65-programmed BMDCs are recruited to the subretinal space, where they repopulate the RPE layer, preserve the photoreceptor layer, retain the thickness of the neural retina, reduce lipofuscin granule formation, and suppress microgliosis...
February 12, 2017: Molecular Therapy: the Journal of the American Society of Gene Therapy
https://www.readbyqxmd.com/read/28202006/differences-in-serum-oxidative-status-between-glaucomatous-and-nonglaucomatous-cataract-patients
#13
Wojciech Rokicki, Jolanta Zalejska-Fiolka, Dorota Pojda-Wilczek, Alicja Hampel, Wojciech Majewski, Serap Ogultekin, Ewa Mrukwa-Kominek
BACKGROUND: Oxidative stress contributes to both intraocular pressure regulation and glaucomatous neuropathy. The systemic redox status (solitary determination) was examined in primary open-angle glaucoma (POAG) patients with cataract and nonglaucomatous cataract patients. Cataract-matched group comparisons appear more precise in the context of oxidative stress evaluation. The aim of this study was to establish if systemic oxidative status in POAG patients was elevated compared with the cataract only subjects...
February 15, 2017: BMC Ophthalmology
https://www.readbyqxmd.com/read/28192797/aberrant-buildup-of-all-trans-retinal-dimer-a-nonpyridinium-bisretinoid-lipofuscin-fluorophore-contributes-to-the-degeneration-of-the-retinal-pigment-epithelium
#14
Junli Zhao, Yi Liao, Jingmeng Chen, Xinran Dong, Zhan Gao, Houjian Zhang, Xiaodan Wu, Zuguo Liu, Yalin Wu
Purpose: Nondegradable fluorophores that accumulate as deleterious lipofuscin of RPE are involved in pathological mechanisms leading to the degeneration of RPE in AMD. A2E, a major component of RPE lipofuscin, could cause damage to RPE cells. Nevertheless, all-trans-retinal dimer (atRAL dimer) was found to be much more abundant than that of A2E in eyes of Abca4-/-Rdh8-/- double-knockout (DKO) mice, a rodent model showing the typical characteristics of retinopathies in AMD patients. Our aim was to elucidate the effect and mechanism of atRAL dimer-induced RPE degeneration...
February 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/28190922/histopathological-background-data-of-the-systemic-organs-of-clawn-miniature-swine-with-coronary-artery-stent-implantation
#15
Atsushi Isobe, Masako Tasaki, Tomoki Inoue, Hisako Terao, Junko Souba, Kasuke Nagano, Hitomi Hagiwara
The aim of this study was to identify potential changes that could occur during histological evaluations of CLAWN miniature swine, with potential consequences for subsequent experiments. The systemic organs from male and female CLAWN miniature swine (16.3-42.3 months old) that had been used in long-term studies of coronary stent implantation were examined histologically. Commonly observed histopathological findings were testicular/epididymal atrophy, cyst-like follicles in the ovaries, hemosiderin deposition in the spleen, lipofuscin deposition in the proximal tubular epithelia and presence of eosinophilic globules in the Bowman's space and the lumen of the proximal tubules in the kidneys, and cellular infiltration in several organs, including the eyelids, respiratory organs, and digestive tract...
January 2017: Journal of Toxicologic Pathology
https://www.readbyqxmd.com/read/28185406/detecting-senescence-a-new-method-for-an-old-pigment
#16
Hanna Salmonowicz, João F Passos
Cellular senescence is a state of irreversible cell cycle arrest induced by different types of cellular stresses. The field of senescence has made significant advances in the understanding of many of the mechanisms governing this phenomenon; however, a universal biomarker that unambiguously distinguishes senescent from proliferating cells has not been found. In this issue of Aging Cell, Evangelou and colleagues developed a sensitive method for identification of senescent cells in different types of biological material based on the detection of lipofuscin using an analogue of Sudan Black B (SBB) histochemical dye coupled with biotin, which they named GL13...
February 9, 2017: Aging Cell
https://www.readbyqxmd.com/read/28165661/robust-universal-biomarker-assay-to-detect-senescent-cells-in-biological-specimens
#17
Konstantinos Evangelou, Nikolaos Lougiakis, Sophia V Rizou, Athanassios Kotsinas, Dimitris Kletsas, Daniel Muñoz-Espín, Nikolaos G Kastrinakis, Nicole Pouli, Panagiotis Marakos, Paul Townsend, Manuel Serrano, Jiri Bartek, Vassilis G Gorgoulis
Cellular senescence contributes to organismal development, aging, and diverse pathologies, yet available assays to detect senescent cells remain unsatisfactory. Here, we designed and synthesized a lipophilic, biotin-linked Sudan Black B (SBB) analogue suitable for sensitive and specific, antibody-enhanced detection of lipofuscin-containing senescent cells in any biological material. This new hybrid histo-/immunochemical method is easy to perform, reliable, and universally applicable to assess senescence in biomedicine, from cancer research to gerontology...
February 2017: Aging Cell
https://www.readbyqxmd.com/read/28160744/mitochondrial-contribution-to-lipofuscin-formation
#18
Jeannette König, Christiane Ott, Martín Hugo, Tobias Jung, Anne-Laure Bulteau, Tilman Grune, Annika Höhn
Mitochondria have been in the focus of oxidative stress and aging research for decades due to their permanent production of ROS during the oxidative phosphorylation. The hypothesis exists that mitochondria are involved in the formation of lipofuscin, an autofluorescent protein aggregate that accumulates progressively over time in lysosomes of post-mitotic and senescent cells. To investigate the influence and involvement of mitochondria in lipofuscinogenesis, we analyzed lipofuscin amounts as well as the mitochondrial function in young and senescent cells...
April 2017: Redox Biology
https://www.readbyqxmd.com/read/28155143/autofluorescence-spectroscopy-for-monitoring-metabolism-in-animal-cells-and-tissues
#19
Anna C Croce, Giovanni Bottiroli
Excitation of biological substrates with light at a suitable wavelength can give rise to a light emission in the ultraviolet (UV)-visible, near-infrared (IR) spectral range, called autofluorescence (AF). This is a widespread phenomenon, ascribable to the general presence of biomolecules acting as endogenous fluorophores (EFs) in the organisms of the whole life kingdom. In cytochemistry and histochemistry, AF is often an unwanted signal enhancing the background and affecting in particular the detection of low signals or rare positive labeling spots of exogenous markers...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28147216/an-emerging-role-of-alu-rna-in-geographic-atrophy-pathogenesis-the-implication-for-novel-therapeutic-strategies
#20
Xiying Mao, Wangyi Fang, Qinghuai Liu
It is generally accepted that geographic atrophy (GA), a currently untreatable advanced form of age-related macular degeneration (AMD), is a multifactorial disease resulting in gradual and permanent blindness. Various risk factors are demonstrated to be responsible for its pathogenesis, such as aging, light exposure, and smoking. Molecular components associated with those risk factors form a complex and interwoven network at the confluence of inflammation, highlighting the significance of inflammasome activation in GA progression...
December 2016: Discovery Medicine
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