keyword
MENU ▼
Read by QxMD icon Read
search

Lipofuscin

keyword
https://www.readbyqxmd.com/read/28423534/estrogen-deficiency-is-associated-with-hippocampal-morphological-remodeling-of-early-postmenopausal-mice
#1
Yan Yan, Liang Cheng, Xin Chen, Qin Wang, Mingjing Duan, Jichao Ma, Linjing Zhao, Xuemei Jiang, Jing Ai
Estrogen (E2) deficiency is reported to involve in the impairment of cognition in postmenopausal women. However, the morphological basis is still unclear. In the present study, using transmission electron microscopy (TEM), we observed the ultrastructure of hippocampus in female C57BL/6 mice at the age of 18 months (18 M) which is considered as the early stage of postmenopause (n = 8). Compared with control mice aged 6 M (n = 8), we identified that the morphological changes in the hippocampus of these menopausal mice were mitochondrial damage, lipofuscin deposition and microtubule degradation...
March 28, 2017: Oncotarget
https://www.readbyqxmd.com/read/28421434/estimation-of-fluorescence-lifetime-of-lipofuscin-fluorophores-contained-in-lipofuscin-granules-of-retinal-pigment-epithelium-of-human-cadaver-eyes-without-signs-of-pathology
#2
M A Yakovleva, T B Feldman, P M Arbukhanova, S A Borzenok, V A Kuzmin, M A Ostrovsky
The fluorescence lifetimes of lipofuscin fluorophores contained in chloroform extracts from retinal pigment epithelium (RPE) of human cadaver eyes without signs of pathology were evaluated by single photon counting. The comparison of fluorescence lifetimes of N-retinylidene-N-retinylethanolamine (A2E) and its photooxidation and photodegradation products has been carried out. It was shown that the contribution of A2E to the total fluorescence of chloroform extract from lipofuscin granules is not major. The results are important for the improvement of noninvasive diagnostic method of degenerative diseases of the retina and RPE-fundus autofluorescence (FAF)...
January 2017: Doklady. Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28407771/amyloid-precursor-protein-lipofuscin-accumulation-and-expression-of-autophagy-markers-in-aged-bovine-brain
#3
D De Biase, A Costagliola, T B Pagano, G Piegari, S Wojcik, J Dziewiątkowski, E Grieco, G Mattace Raso, V Russo, S Papparella, O Paciello
BACKGROUND: Autophagy is a highly regulated process involving the bulk degradation of cytoplasmic macromolecules and organelles in mammalian cells via the lysosomal system. Dysregulation of autophagy is implicated in the pathogenesis of many neurodegenerative diseases and integrity of the autophagosomal - lysosomal network appears to be critical in the progression of aging. Our aim was to survey the expression of autophagy markers and Amyloid precursor protein (APP) in aged bovine brains...
April 13, 2017: BMC Veterinary Research
https://www.readbyqxmd.com/read/28401421/redox-active-transition-metal-ions-make-melanin-susceptible-to-chemical-degradation-induced-by-organic-peroxide
#4
Andrzej Zadlo, Anna Pilat, Michal Sarna, Anna Pawlak, Tadeusz Sarna
With aging, retinal pigment epithelium melanosomes, by fusion with the age pigment lipofuscin, form complex granules called melanolipofuscin. Lipofuscin granules may contain oxidized proteins and lipid hydroperoxides, which in melanolipofuscin could chemically modify melanin polymer, while transition metal ions present in melanin can accelerate such oxidative modifications. The aim of this research was to examine the effect of selected transition metal ions on melanin susceptibility to chemical modification induced by the water-soluble tert-butyl hydroperoxide used as an oxidizing agent...
April 11, 2017: Cell Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28395299/multimodal-fundus-imaging-of-sodium-iodate-treated-mice-informs-rpe-susceptibility-and-origins-of-increased-fundus-autofluorescence
#5
Jin Zhao, Hye Jin Kim, Janet R Sparrow
Purpose: By multimodal imaging, and the use of mouse and in vitro models, we have addressed changes in fundus autofluorescence (488 and 790 nm) and observed interactions between the photooxidative stress imposed by RPE bisretinoid lipofuscin and the oxidative impact of systemic sodium iodate (NaIO3). Methods: Abca4-/-, wild-type, and Rpe65rd12 mice were given systemic injections of NaIO3 (30 mg/kg). Analysis included noninvasive imaging of fundus autofluorescence (short-wavelength [SW-AF]; near-infrared excitation [NIR-AF]), quantitative fundus AF (qAF; 488 nm); light microscopy, RPE flat-mounts and measurements of outer nuclear layer (ONL) thickness...
April 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/28353645/inhibition-or-stimulation-of-autophagy-affects-early-formation-of-lipofuscin-like-autofluorescence-in-the-retinal-pigment-epithelium-cell
#6
Lei Lei, Radouil Tzekov, Huapeng Li, J Hugh McDowell, Guangping Gao, W Clay Smith, Shibo Tang, Shalesh Kaushal
The accumulation of lipofuscin in the retinal pigment epithelium (RPE) is dependent on the effectiveness of photoreceptor outer segment material degradation. This study explored the role of autophagy in the fate of RPE lipofuscin degradation. After seven days of feeding with either native or modified rod outer segments, ARPE-19 cells were treated with enhancers or inhibitors of autophagy and the autofluorescence was detected by fluorescence-activated cell sorting. Supplementation with different types of rod outer segments increased lipofuscin-like autofluorescence (LLAF) after the inhibition of autophagy, while the induction of autophagy (e...
March 29, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28351686/dna-damage-response-and-autophagy-in-the-degeneration-of-retinal-pigment-epithelial-cells-implications-for-age-related-macular-degeneration-amd
#7
REVIEW
Juha M T Hyttinen, Janusz Błasiak, Minna Niittykoski, Kati Kinnunen, Anu Kauppinen, Antero Salminen, Kai Kaarniranta
In this review we will discuss the links between autophagy, a mechanism involved in the maintenance of cellular homeostasis and controlling cellular waste management, and the DNA damage response (DDR), comprising various mechanisms preserving the integrity and stability of the genome. A reduced autophagy capacity in retinal pigment epithelium has been shown to be connected in the pathogenesis of age-related macular degeneration (AMD), an eye disease. This degenerative disease is a major and increasing cause of vision loss in the elderly in developed countries, primarily due to the profound accumulation of intra- and extracellular waste: lipofuscin and drusen...
March 27, 2017: Ageing Research Reviews
https://www.readbyqxmd.com/read/28348233/complement-modulation-in-the-retinal-pigment-epithelium-rescues-photoreceptor-degeneration-in-a-mouse-model-of-stargardt-disease
#8
Tamara L Lenis, Shanta Sarfare, Zhichun Jiang, Marcia B Lloyd, Dean Bok, Roxana A Radu
Recessive Stargardt macular degeneration (STGD1) is caused by mutations in the gene for the ABCA4 transporter in photoreceptor outer segments. STGD1 patients and Abca4(-/-) (STGD1) mice exhibit buildup of bisretinoid-containing lipofuscin pigments in the retinal pigment epithelium (RPE), increased oxidative stress, augmented complement activation and slow degeneration of photoreceptors. A reduction in complement negative regulatory proteins (CRPs), possibly owing to bisretinoid accumulation, may be responsible for the increased complement activation seen on the RPE of STGD1 mice...
April 11, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28319893/role-of-macrophages-in-age-related-oxidative-stress-and-lipofuscin-accumulation-in-mice
#9
Carmen Vida, Irene Martínez de Toda, Julia Cruces, Antonio Garrido, Mónica Gonzalez-Sanchez, Mónica De la Fuente
The age-related changes in the immune functions (immunosenescence) may be mediated by an increase of oxidative stress and damage affecting leukocytes. Although the "oxidation-inflammation" theory of aging proposes that phagocytes are the main immune cells contributing to "oxi-inflamm-aging", this idea has not been corroborated. The aim of this work was to characterize the age-related changes in several parameters of oxidative stress and immune function, as well as in lipofuscin accumulation ("a hallmark of aging"), in both total peritoneal leukocyte population and isolated peritoneal macrophages...
March 9, 2017: Redox Biology
https://www.readbyqxmd.com/read/28301413/ophthalmic-findings-in-late-stage-sjogren-larsson-syndrome
#10
Tavish Nanda, Jaclyn L Kovach
PURPOSE: To report spectral domain optical coherence tomography and fundus autofluorescence documentation of late stage macular findings associated with Sjogren-Larsson Syndrome in three adult siblings. METHODS: Three adult siblings with Sjogren-Larsson Syndrome underwent ophthalmic examination and imaging. RESULTS: Crystalline maculopathy and subretinal deposits, presumably lipofuscin accumulation, with macular atrophy were present in varying degrees in all three adult siblings...
March 15, 2017: Retinal Cases & Brief Reports
https://www.readbyqxmd.com/read/28299497/multimodal-imaging-in-a-case-of-butterfly-pattern-dystrophy-of-retinal-pigment-epithelium
#11
Vinod Kumar, Devesh Kumawat
AIMS: To report multi-modal imaging findings in a case of butterfly pattern dystrophy of retinal pigment epithelium. METHODS: A middle-aged female with butterfly pattern dystrophy, who presented with progressive loss of vision, was examined using coloured fundus photographs, short wave autofluorescence, swept source optical coherence tomography (SS-OCT), fundus fluorescein angiography and enface optical coherence tomography. RESULTS: Multi-modal imaging was useful in the characterization of the various disease features...
March 15, 2017: International Ophthalmology
https://www.readbyqxmd.com/read/28298893/bis-retinoid-a2e-induces-an-increase-of-basic-fibroblast-growth-factor-via-inhibition-of-extracellular-signal-regulated-kinases-1-2-pathway-in-retinal-pigment-epithelium-cells-and-facilitates-phagocytosis
#12
Delphine Balmer, Linda Bapst-Wicht, Aswin Pyakurel, Martine Emery, Natacha Nanchen, Christian G Bochet, Raphael Roduit
Age-related macular degeneration (ARMD) is the leading cause of vision loss in developed countries. Hallmarks of the disease are well known; indeed, this pathology is characterized by lipofuscin accumulation, is principally composed of lipid-containing residues of lysosomal digestion. The N-retinyl-N-retinylidene ethanolamine (A2E) retinoid which is thought to be a cytotoxic component for RPE is the best-characterized component of lipofuscin so far. Even if no direct correlation between A2E spatial distribution and lipofuscin fluorescence has been established in aged human RPE, modified forms or metabolites of A2E could be involved in ARMD pathology...
2017: Frontiers in Aging Neuroscience
https://www.readbyqxmd.com/read/28291154/assessment-of-drusen-and-other-retinal-degenerative-changes-in-patients-with-hereditary-hemochromatosis
#13
Moreno Menghini, Christian Prünte, Pierre A Krayenbuehl, Albina Nowak
PURPOSE: Iron can exert oxidative damage, and increased accumulation is believed to play a role in age-related macular degeneration. Hereditary hemochromatosis leads to an increase in total body iron. Patients with HH were assessed for drusen and other retinal changes. METHODS: Descriptive uncontrolled study of spectral-domain optical coherence tomography, short-wavelength autofluorescence, and color fundus images from patients with HH were used. Diagnosis of HH was established by measuring ferritin and transferrin saturation, and confirmed by genetic testing...
March 13, 2017: Retina
https://www.readbyqxmd.com/read/28282423/age-related-macular-degeneration-phenotypes-are-associated-with-increased-tumor-necrosis-alpha-and-subretinal-immune-cells-in-aged-cxcr5-knockout-mice
#14
Hu Huang, Ying Liu, Lei Wang, Wen Li
The role of chemokine receptor in age-related macular degeneration (AMD) remains elusive. The objective of this study is to investigate the role of chemokine receptor Cxcr5 in the pathogenesis of AMD. Cxcr5 gene expression levels (mRNA and protein) are higher in the retina and retinal pigment epithelium (RPE) of aged C57BL/6 wild type mice than younger ones. Vascular and glial cells express Cxcr5 and its ligand Cxcl13 in mouse retina. Aged Cxcr5 knockout (-/-) mice develop both early and late AMD-like pathological features...
2017: PloS One
https://www.readbyqxmd.com/read/28267565/automating-cell-detection-and-classification-in-human-brain-fluorescent-microscopy-images-using-dictionary-learning-and-sparse-coding
#15
Maryana Alegro, Panagiotis Theofilas, Austin Nguy, Patricia A Castruita, William Seeley, Helmut Heinsen, Daniela M Ushizima, Lea T Grinberg
BACKGROUND: Immunofluorescence (IF) plays a major role in quantifying protein expression in situ and understanding cell function. It is widely applied in assessing disease mechanisms and in drug discovery research. Automation of IF analysis can transform studies using experimental cell models. However, IF analysis of postmortem human tissue relies mostly on manual interaction, often subjected to low-throughput and prone to error, leading to low inter and intra-observer reproducibility...
April 15, 2017: Journal of Neuroscience Methods
https://www.readbyqxmd.com/read/28260365/-ultrastructural-alteration-of-extraocular-muscle-proprioceptor-in-congenital-idiopathic-nystagmus
#16
C Y Zhang, D Y Bai, Y H Cui, S L Hu, Y Zhang, C Li, L Li
Objective: To explore the ultrastructural alteration of extraocular muscle proprioceptor in congenital idiopathic nystagmus (CIN). Methods: Case-control study. Ten extraocular muscle samples were collected from five CIN children who underwent nystagmus surgeries in Beijing Children's Hospital from March 2015 to March 2016. Another ten extraocular muscle specimens were collected from five strabismus children in surgery at the same period as normal contrast. There were 3 male patients and 2 female patients of CIN with age of 61-147 months (median age: 91 months)...
February 11, 2017: [Zhonghua Yan Ke za Zhi] Chinese Journal of Ophthalmology
https://www.readbyqxmd.com/read/28244572/anti-aging-effect-of-riboflavin-via-endogenous-antioxidant-in-fruit-fly-drosophila-melanogaster
#17
Y-X Zou, M-H Ruan, J Luan, X Feng, S Chen, Z-Y Chu
OBJECTIVES: This study investigated the effect of riboflavin on aging in Drosophila melanogaster (fruit fly). DESIGN: Experimental study. SETTING: Naval Medical Research Institute. PARTICIPANTS: Fruit fly Drosophila melanogaster. INTERVENTION: After lifelong supplement of riboflavin, the lifespan and the reproduction of fruit flies were observed. Hydrogen peroxide (H2O2) was used to mimic oxidative stress damage to fruit flies and the survival time was recorded...
2017: Journal of Nutrition, Health & Aging
https://www.readbyqxmd.com/read/28226256/endocrine-and-cellular-stress-effects-of-zinc-oxide-nanoparticles-and-nifedipine-in-marsh-frogs-pelophylax-ridibundus
#18
Halina Falfushynska, Lesya Gnatyshyna, Oksana Horyn, Inna Sokolova, Oksana Stoliar
Freshwater organisms including amphibians experience increasing exposures to emerging pollutants such as nanoparticles and pharmaceuticals, which can affect their fitness and performance. We studied the effects of two common pollutants extensively used in industry, pharmaceutical and personal care products, nano-zinc oxide (nZnO) and a Ca-channel blocker nifedipine (Nfd), on endocrine status and cellular stress markers of the marsh frog Pelophylax ridibundus. Males were exposed for 14days to nZnO (3.1μM), Zn(2+) (3...
April 2017: Aquatic Toxicology
https://www.readbyqxmd.com/read/28219732/all-trans-retinal-levels-and-formation-of-lipofuscin-precursors-after-bleaching-in-rod-photoreceptors-from-wild-type-and-abca4-mice
#19
Leopold Adler, Chunhe Chen, Yiannis Koutalos
The accumulation of lipofuscin in the cells of the retinal pigment epithelium (RPE) is thought to play an important role in the development and progression of degenerative diseases of the retina. The bulk of RPE lipofuscin originates in reactions of the rhodopsin chromophore, retinal, with components of the photoreceptor outer segment. The 11-cis retinal isomer is generated in the RPE and supplied to rod photoreceptor outer segments where it is incorporated as the chromophore of rhodopsin. It is photoisomerized during light detection to all-trans and subsequently released by photoactivated rhodopsin as all-trans retinal, which is removed through reduction to all-trans retinol in a reaction requiring metabolic input in the form of NADPH...
February 2017: Experimental Eye Research
https://www.readbyqxmd.com/read/28202390/systemic-injection-of-rpe65-programmed-bone-marrow-derived-cells-prevents-progression-of-chronic-retinal-degeneration
#20
Xiaoping Qi, S Louise Pay, Yuanqing Yan, James Thomas, Alfred S Lewin, Lung-Ji Chang, Maria B Grant, Michael E Boulton
Bone marrow stem and progenitor cells can differentiate into a range of non-hematopoietic cell types, including retinal pigment epithelium (RPE)-like cells. In this study, we programmed bone marrow-derived cells (BMDCs) ex vivo by inserting a stable RPE65 transgene using a lentiviral vector. We tested the efficacy of systemically administered RPE65-programmed BMDCs to prevent visual loss in the superoxide dismutase 2 knockdown (Sod2 KD) mouse model of age-related macular degeneration. Here, we present evidence that these RPE65-programmed BMDCs are recruited to the subretinal space, where they repopulate the RPE layer, preserve the photoreceptor layer, retain the thickness of the neural retina, reduce lipofuscin granule formation, and suppress microgliosis...
April 5, 2017: Molecular Therapy: the Journal of the American Society of Gene Therapy
keyword
keyword
114328
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"