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https://www.readbyqxmd.com/read/28535008/how-much-medium-do-you-use-for-cell-culture-medium-volume-influences-mineralization-and-osteoclastogenesis-in%C3%A2-vitro
#1
Yoshitaka Yoshimura, Takashi Kikuiri, Tomokazu Hasegawa, Mino Matsuno, Hajime Minamikawa, Yoshiaki Deyama, Kuniaki Suzuki
Bone is maintained by a balance between bone formation and resorption. This remodeling is controlled by a wide variety of systemic and local factors including hormones, cytokines and mechanical stresses. The present in vitro study examined the impact of medium volume, using 0.4, 0.6, 0.8, 1.0, 1.5 and 2.0 ml/well in a 24‑well plate, on the differentiation of osteoblasts and osteoclasts. There were no differences in the alkaline phosphatase activity of osteoblasts amongst the groups; however, the area of mineral deposition was decreased in a media volume‑dependent manner...
May 19, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28533432/the-atp-dependent-chromatin-remodeling-enzymes-chd6-chd7-and-chd8-exhibit-distinct-nucleosome-binding-and-remodeling-activities
#2
Benjamin J Manning, Timur Yusufzai
Proper chromatin regulation is central to genome function and maintenance. The group III CHD family of ATP-dependent chromatin remodeling enzymes--comprising CHD6, CHD7, CHD8, and CHD9--has well-documented roles in transcription regulation impacting both organism development and disease etiology. These four enzymes are similar in their constituent domains, yet these enzymes fill surprisingly non-redundant roles in the cell, with deficiencies in individual enzymes leading to dissimilar disease states such as CHARGE syndrome or autism spectrum disorders...
May 21, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28528743/-18-f-sodium-fluoride-positron-emission-tomography-for-molecular-imaging-of-coronary-atherosclerosis-based-on-computed-tomography-analysis
#3
Toshiro Kitagawa, Hideya Yamamoto, Shinya Toshimitsu, Ko Sasaki, Atsuhiro Senoo, Yumiko Kubo, Fuminari Tatsugami, Kazuo Awai, Yutaka Hirokawa, Yasuki Kihara
BACKGROUND AND AIMS: We aimed at evaluating the relation of (18)F-sodium fluoride ((18)F-NaF) uptake on positron emission tomography (PET) to coronary atherosclerosis detected and assessed by computed tomography (CT). METHODS: Thirty-two patients with one or more coronary atherosclerotic lesions detected on cardiac CT underwent (18)F-NaF PET/CT. Each coronary atherosclerotic lesion was evaluated on CT angiography for plaque types (calcified plaque [CP], non-calcified plaque [NCP], partially calcified plaque [PCP]), and the presence of CT-based high-risk features (minimum CT density <30 Hounsfield units and vascular remodeling index >1...
April 29, 2017: Atherosclerosis
https://www.readbyqxmd.com/read/28528430/risk-classification-of-highly-sensitive-troponin-i-predict-presence-of-vulnerable-plaque-assessed-by-dual-source-coronary-computed-tomography-angiography
#4
Ting Liu, Guan Wang, Peiling Li, Xu Dai
Patients presenting to the emergency department with acute chest pain, negative conventional troponin and electrocardiogram require serial testing to rule out acute coronary syndrome (ACS). We studied the association of highly sensitive troponin (hsTn) I with vulnerable plaque features as detected by coronary dual source computed tomography angiography (DSCTA) and determined whether hsTn I at the time of presentation combined with early DSCTA could improve classification of patients as high-risk or low risk for ACS...
May 20, 2017: International Journal of Cardiovascular Imaging
https://www.readbyqxmd.com/read/28528367/evaluating-the-role-of-autologous-mesenchymal-stem-cell-seeded-on-decellularized-pericardium-in-the-treatment-of-myocardial-infarction-an-animal-study
#5
Abdol-Mohammad Kajbafzadeh, Seyed Hossein Ahmadi Tafti, Reza Khorramirouz, Shabnam Sabetkish, Seyedeh Maryam Kameli, Saghar Orangian, Shahram Rabbani, Nasim Oveisi, Marziyeh Golmohammadi, Zeynab Kashani
Inappropriate left ventricular remodeling following myocardial infarction (MI) can result in subsequent severe dysfunction. In this study, we tested the hypothesis that decellularized pericardium (DP) or seeded pericardial patch with autologous adipose-derived mesenchymal stem cells (ADMSCs) could be safely used in a MI scar and could improve heart function. Twelve rabbits were randomly divided into three equal groups. Four weeks after MI induction by ligation of the left anterior descending artery in 12 rabbits, animals of G1 (n = 4) received DP patch with labeled ADMSCs...
May 20, 2017: Cell and Tissue Banking
https://www.readbyqxmd.com/read/28526017/the-effect-of-hypoxia-on-the-lipidome-of-recombinant-pichia-pastoris
#6
Núria Adelantado, Pablo Tarazona, Karlheinz Grillitsch, Xavier García-Ortega, Sergi Monforte, Francisco Valero, Ivo Feussner, Günther Daum, Pau Ferrer
BACKGROUND: Cultivation of recombinant Pichia pastoris (Komagataella sp.) under hypoxic conditions has a strong positive effect on specific productivity when the glycolytic GAP promoter is used for recombinant protein expression, mainly due to upregulation of glycolytic conditions. In addition, transcriptomic analyses of hypoxic P. pastoris pointed out important regulation of lipid metabolism and unfolded protein response (UPR). Notably, UPR that plays a role in the regulation of lipid metabolism, amino acid metabolism and protein secretion, was found to be upregulated under hypoxia...
May 19, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28521792/m2-macrophage-is-the-predominant-phenotype-in-airways-inflammatory-lesions-in-patients-with-granulomatosis-with-polyangiitis
#7
Alexandre Wagner Silva de Souza, Mirjan van Timmeren, Jan-Stephan Sanders, Coen Stegeman, Peter Heeringa, Cees G M Kallenberg, Johanna Westra
BACKGROUND: Macrophages may present two distinct phenotypes indicated as M1 and M2 under different stimuli. M1 and M2 macrophages have divergent functions that range from enhancement of inflammation for M1 to tissue repair and remodeling for M2 macrophages. The objective of this study was to evaluate the distribution of M1 and M2 macrophage phenotypes in biopsies from the airways of patients with active granulomatosis with polyangiitis (GPA) and to analyze their associations with T and B cells in those biopsies, and with nasal carriage of Staphylococcus aureus, disease parameters and therapy...
May 18, 2017: Arthritis Research & Therapy
https://www.readbyqxmd.com/read/28518186/molecular-activity-and-osseointegration-after-single-dose-irradiation-an-in-vivo-study
#8
Jan Nyberg, Gisela Helenius, Christer Dahlin, Carina B Johansson, Omar Omar
PURPOSE: Irradiation results in deleterious effects on bone healing and integration of titanium implants. The impact of irradiation on osseointegration has been demonstrated in histologic studies, but the underlying molecular mechanisms have not been explored. This study aimed to investigate the effects of single-dose irradiation on the expression of biologic mediators crucial for inflammation, bone formation, and bone remodeling and to relate these molecular activities to implant stability after a 5-week healing period...
May 18, 2017: International Journal of Oral & Maxillofacial Implants
https://www.readbyqxmd.com/read/28513656/restoring-the-biophysical-properties-of-decellularized-patches-through-recellularization
#9
Gigi Chi Ting Au-Yeung, Udi Sarig, Hadar Sarig, Hanumakumar Bogireddi, Tomer Bronshtein, Limor Baruch, Avihai Spizzichino, Jacob Bortman, Boey Yin Chiang Freddy, Marcelle Machluf, Subbu S Venkatraman
Various extracellular matrix (ECM) scaffolds, isolated through decellularization, were suggested as ideal biomimetic materials for 'Functional tissue engineering' (FTE). The decellularization process comprises a compromise between damaging and preserving the ultrastructure and composition of ECM-previously shown to affect cell survival, proliferation, migration, organization, differentiation and maturation. Inversely, the effects of cells on the ECM constructs' biophysical properties, under physiological-like conditions, remain still largely unknown...
May 17, 2017: Biomaterials Science
https://www.readbyqxmd.com/read/28508868/influence-of-age-and-eosinophilic-esophagitis-on-esophageal-distensibility-in-a-pediatric-cohort
#10
Calies Menard-Katcher, Alain J Benitez, Zhaoxing Pan, Faria N Ahmed, Benjamin J Wilkins, Kelley E Capocelli, Chris A Liacouras, Ritu Verma, Jonathan M Spergel, Glenn T Furuta, Amanda B Muir
OBJECTIVES: Sequelae of eosinophilic esophagitis (EoE) include food impaction and esophageal stricture. Duration of inflammation is a predicted risk factor; however, complications remain unpredictable. Studies using the functional lumen imaging probe (FLIP) have demonstrated decreased distensibility of the esophagus in adult patients with EoE. As the impact of inflammation on the developing esophagus is unknown, we investigated esophageal distensibility in a pediatric cohort to determine the effect of age, ongoing inflammation, and fibrotic features on distensibility...
May 16, 2017: American Journal of Gastroenterology
https://www.readbyqxmd.com/read/28508565/menaquinone-4-enhances-osteogenic-potential-of-human-amniotic-fluid-mesenchymal-stem-cells-cultured-in-a-2d-and-3d-dynamic-culture-system
#11
Domitilla Mandatori, Letizia Penolazzi, Caterina Pipino, Pamela Di Tomo, Sara Di Silvestre, Natalia Di Pietro, Sara Trevisani, Marco Angelozzi, Mariangela Ucci, Roberta Piva, Assunta Pandolfi
Menaquinones, also known as Vitamin K2 family, regulate calcium homeostasis in a "bone-vascular cross talk" and recently received particular attention for their positive effect on bone formation. Since the correlation between menaquinones and bone metabolism to date is still unclear, the objective of our study was to investigate the possible role of menaquinone-4 (MK-4), an isoform of the menaquinones family, in the modulation of osteogenesis. For this reason we used a model of human Amniotic Fluid Mesenchymal Stem Cells (hAFMSCs) cultured both in two-dimensional (2D) and three-dimensional (3D, RCCS™bioreactor) in vitro culture systems...
May 16, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28508515/extra-oral-digital-scanning-and-imaging-superimposition-for-volume-analysis-of-bone-remodeling-after-tooth-extraction-with-and-without-2-types-of-particulate-porcine-mineral-insertion-a-randomized-controlled-trial
#12
Antonio Barone, Paolo Toti, Giovan-Battista Menchini-Fabris, Giacomo Derchi, Simone Marconcini, Ugo Covani
BACKGROUND: Incorporation of bone substitute material into the extraction socket could minimize the edentulous ridge volume loss or maximize the bone formation within the healing area. PURPOSE: The aim of the study was to test the effectiveness in maintaining the volumetric contour of fresh extraction sockets grafted with or without 2 types of porcine biomaterials and covered with a resorbable barrier. MATERIALS AND METHODS: In the present multi-center single-blind randomized control trial, 55 patients underwent 1-tooth extraction, were surveyed, and randomly allocated to control- (25 sites, nat-group) or test groups (15 sites grafted with pre-hydrated collagenated cortico-cancellous porcine bone, coll-group, and 15 with cortical porcine bone, cort-group)...
May 16, 2017: Clinical Implant Dentistry and related Research
https://www.readbyqxmd.com/read/28507585/ctgf-upregulation-correlates-with-mmp-9-level-in-airway-remodeling-in-a%C3%A2-murine-model-of-asthma
#13
Sheng-Chieh Lin, Hsiu-Chu Chou, Bor-Luen Chiang, Chung-Ming Chen
INTRODUCTION: Connective tissue growth factor (CTGF) mediates hypertrophy, proliferation, and extracellular matrix synthesis. Matrix metalloproteinase (MMP) plays a role in airway extracellular matrix remodeling. The correlation between CTGF and MMP in airway remodeling of asthma was unknown. This study investigated lung CTGF expression and its correlation with MMP and airway structural changes in a murine model of asthma. MATERIAL AND METHODS: Female BALB/c mice were sensitized and challenged by intraperitoneal injections and intranasal phosphate-buffered saline (PBS) or ovalbumin (OVA)...
April 1, 2017: Archives of Medical Science: AMS
https://www.readbyqxmd.com/read/28506627/rxrb-is-a-mhc-encoded-susceptibility-gene-associated-with-anti-topoisomerase-i-antibody-positive-systemic-sclerosis
#14
Akira Oka, Yoshihide Asano, Minoru Hasegawa, Manabu Fujimoto, Osamu Ishikawa, Masataka Kuwana, Yasushi Kawaguchi, Toshiyuki Yamamoto, Hiroki Takahashi, Daisuke Goto, Hirahito Endo, Masatoshi Jinnin, Shuhei Mano, Kazuyoshi Hosomichi, Tomotaka Mabuchi, Mahoko Takahashi Ueda, So Nakagawa, Stephan Beck, Seiamak Bahram, Kazuhiko Takehara, Shinichi Sato, Hironobu Ihn
Systemic sclerosis (SSc) is a systemic autoimmune and connective tissue disorder associated with the human leukocyte antigen (HLA) locus. However, the functional relationship between HLA gene(s) and disease development remains unknown. To elucidate major histocompatibility complex (MHC)-linked SSc genetics, we performed genotyping of MHC-borne microsatellites and HLA-DPB1 alleles using DNA obtained from 318 anti-topoisomerase I antibody-positive patients and 561 healthy controls, all of Japanese descent. Those results revealed 2 MHC haplotypes associated with SSc...
May 12, 2017: Journal of Investigative Dermatology
https://www.readbyqxmd.com/read/28502498/mature-proprotein-convertase-subtilisin-kexin-type-9-coronary-atheroma-burden-and-vessel-remodeling-in-heterozygous-familial-hypercholesterolemia
#15
Yu Kataoka, Mariko Harada-Shiba, Kazuhiro Nakao, Takahiro Nakashima, Shoji Kawakami, Masashi Fujino, Tomoaki Kanaya, Toshiyuki Nagai, Yoshio Tahara, Yasuhide Asaumi, Mika Hori, Masatsune Ogura, Yoichi Goto, Teruo Noguchi, Satoshi Yasuda
BACKGROUND: Proprotein convertase subtilisin/kexin type 9 (PCSK9), an important contributor to low-density lipoprotein metabolism in heterozygous familial hypercholesterolemia (HeFH), exhibits direct proatherogenic effects. PCSK9 circulates as mature and furin-cleaved forms, which differ in its biological activity. However, it remains to be elucidated whether each PCSK9 subtype has different atherogenic properties. OBJECTIVE: To investigate the association of each PCSK9 subtype with coronary atherosclerosis in HeFH...
March 2017: Journal of Clinical Lipidology
https://www.readbyqxmd.com/read/28502095/the-impact-of-age-and-frailty-on-ventricular-structure-and-function-in-c57bl-6j-mice
#16
H A Feridooni, A E Kane, O Ayaz, A Boroumandi, N Polidovitch, R G Tsushima, R A Rose, S E Howlett
KEY POINTS: Heart size increases with age (called hypertrophy), and its ability to contract declines. However, these reflect average changes that may not be present, or present to the same extent, in all older individuals. That aging happens at different rates is well accepted clinically. People who are aging rapidly are frail and frailty is measured with a 'frailty index'. We quantified frailty with a validated mouse frailty index tool and evaluated the impacts of age and frailty on cardiac hypertrophy and contractile dysfunction...
May 14, 2017: Journal of Physiology
https://www.readbyqxmd.com/read/28500378/intravascular-ultrasound-elastography-analysis-of-the-elastic-mechanical-properties-of-atherosclerotic-plaque
#17
Zhaohuan Li, Lin Wang, Xiaobo Hu, Pengfei Zhang, Yifei Chen, Xinxin Liu, Mingjun Xu, Haijun Su, Mei Zhang
To assess the elastic mechanical properties of atherosclerotic plaque with different morphological properties by intravascular ultrasound elastography (IVUSE). 30 purebred New Zealand rabbits were fed a high-cholesterol diet; the abdominal aorta endothelium was balloon-injured after 2 weeks; at week 12, 2 plaques with moderate echo from each rabbit were chosen for in situ imaging, and 2 consecutive frames near the end-diastole images in situ were used to construct an IVUS elastogram. Shear strain (SS) and area strain (AS) were greater for eccentric than centripetal plaque (SS: 2...
May 13, 2017: International Journal of Cardiovascular Imaging
https://www.readbyqxmd.com/read/28494969/rate-dependent-role-of-ikur-in-human-atrial-repolarization-and-atrial-fibrillation-maintenance
#18
Martin Aguilar, Jianlin Feng, Edward Vigmond, Philippe Comtois, Stanley Nattel
The atrial-specific ultrarapid delayed rectifier K(+) current (IKur) inactivates slowly but completely at depolarized voltages. The consequences for IKur rate-dependence have not been analyzed in detail and currently available mathematical action-potential (AP) models do not take into account experimentally observed IKur inactivation dynamics. Here, we developed an updated formulation of IKur inactivation that accurately reproduces time-, voltage-, and frequency-dependent inactivation. We then modified the human atrial cardiomyocyte Courtemanche AP model to incorporate realistic IKur inactivation properties...
May 9, 2017: Biophysical Journal
https://www.readbyqxmd.com/read/28494928/shotgun-proteomic-analysis-of-the-secretome-of-bovine-endometrial-mesenchymal-progenitor-stem-cells-challenged-or-not-with-bacterial-lipopolysaccharide
#19
Carolina Nogueira de Moraes, Leandro Maia, Eliandre de Oliveira, Camila de Paula Freitas Dell'Aqua, Aspinas Chapwanya, Fernanda da Cruz Landim-Alvarenga, Eunice Oba
The use of the conditioned medium (CM) for diseases treatment is based on its enrichment with biomolecules with therapeutic properties and themselves have a beneficial effect. Secretome of bovine endometrial mesenchymal progenitor/stem cells (eMSCs) using a proteomics approach is until now unknown. This work aimed to evaluate the secretome of bovine eMSCs-CM challenged or not with lipopolysaccharide (LPS). For this, eMSCs characterized were challenged (TG) or not (CG). The CM was collected 12h after stimulation and submitted to mass spectrometry analysis...
May 2017: Veterinary Immunology and Immunopathology
https://www.readbyqxmd.com/read/28494457/resveratrol-protects-against-pulmonary-arterial-hypertension-in-rats-via-activation-of-silent-information-regulator-1
#20
Lei Yu, Yingfeng Tu, Xueling Jia, Kun Fang, Li Liu, Lin Wan, Chuanying Xiang, Yanan Wang, Xiangju Sun, Tianyou Liu, Dejun Yu, Weiwei Cao, Yinli Song, Yuhua Fan
BACKGROUND/OBJECTIVES: The polyphenol resveratrol (Rev) has been found to exhibit various beneficial effects including prevention of pulmonary arterial hypertension (PAH). The present study was designed to investigate the action and potential mechanism of Rev on PAH, focusing on the role of SIRT1 (Silent Information Regulator 1) in apoptosis of pulmonary artery smooth muscle cells (PASMCs). METHODS: PAH rats were established by exposure to hypoxia for 21 days. Rev and SRT1720 (a selective SIRT1 activator) were used to reverse PAH by gavaging rats...
May 11, 2017: Cellular Physiology and Biochemistry
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