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https://www.readbyqxmd.com/read/28145107/a-comprehensive-experiment-for-molecular-biology-determination-of-single-nucleotide-polymorphism-in-human-rev3-gene-using-pcr-rflp
#1
Xu Zhang, Meng Shao, Lu Gao, Yuanyuan Zhao, Zixuan Sun, Liping Zhou, Yongmin Yan, Qixiang Shao, Wenrong Xu, Hui Qian
Laboratory exercise is helpful for medical students to understand the basic principles of molecular biology and to learn about the practical applications of molecular biology. We have designed a lab course on molecular biology about the determination of single nucleotide polymorphism (SNP) in human REV3 gene, the product of which is a subunit of DNA polymerase ζ and SNPs in this gene are associated with altered susceptibility to cancer. This newly designed experiment is composed of three parts, including genomic DNA extraction, gene amplification by PCR, and genotyping by RFLP...
February 1, 2017: Biochemistry and Molecular Biology Education
https://www.readbyqxmd.com/read/28107066/hematologic-plasma-biochemical-and-lipid-panel-reference-intervals-in-orange-winged-amazon-parrots-amazona-amazonica
#2
Claire Vergneau-Grosset, Tamsen Polley, Danielle Carrade Holt, William Vernau, Joanne Paul-Murphy
To establish reference intervals in orange-winged Amazon parrots ( Amazona amazonica ) for the complete blood count, plasma biochemical values, and lipid panel and to evaluate age- and sex-related variations, blood samples were obtained from 29 healthy juvenile and adult parrots. Concentrations of total protein, bile acids, phosphorus, total cholesterol, low-density-lipoprotein cholesterol (LDL-C), and high-density lipoprotein cholesterol were significantly higher in adult compared with juvenile birds, while uric acid concentration was significantly higher in juveniles...
December 2016: Journal of Avian Medicine and Surgery
https://www.readbyqxmd.com/read/28032403/a-unique-large-scale-undergraduate-research-experience-in-molecular-systems-biology-for-non-mathematics-majors
#3
Ulrike Kappler, Susan L Rowland, Rhianna K Pedwell
Systems biology is frequently taught with an emphasis on mathematical modeling approaches. This focus effectively excludes most biology, biochemistry, and molecular biology students, who are not mathematics majors. The mathematical focus can also present a misleading picture of systems biology, which is a multi-disciplinary pursuit requiring collaboration between biochemists, bioinformaticians, and mathematicians. This article describes an authentic large-scale undergraduate research experience (ALURE) in systems biology that incorporates proteomics, bacterial genomics, and bioinformatics in the one exercise...
December 28, 2016: Biochemistry and Molecular Biology Education
https://www.readbyqxmd.com/read/28032379/changes-in-cardiovascular-performance-biochemistry-gastric-motility-and-muscle-temperature-induced-by-acute-exercise-on-a-treadmill-in-healthy-military-dogs
#4
R W Queiroz, V L Silva, D R Rocha, D S Costa, S H N Turco, M T B Silva, A A Santos, M B L Oliveira, A S R Pereira, R C Palheta-Junior
Changes in physiological parameters that are induced by acute exercise on a treadmill in healthy military dogs have not been thoroughly investigated, especially with regard to age. This study investigated the effects of acute exercise on a treadmill on cardiovascular function, biochemical parameters and gastric antral motility in military dogs. Thermography was used to assess variations in superficial hindlimb muscle temperature. Nine healthy dogs were distributed into three groups according to their age (Group I: 25 ± 7 months; Group II: 51 ± 12 months; Group III: 95 ± 10 months) and sequentially subjected to running exercise on a treadmill for 12 min (3...
December 29, 2016: Journal of Animal Physiology and Animal Nutrition
https://www.readbyqxmd.com/read/28007443/translational-misreading-amino-acid-misincorporation-and-misinterpretations-the-case-of-the-flavocytochrome-b2-h373q-variant
#5
Mouna Ben Elhoul, Paul Machillot, Mireille Benoît, Florence Lederer
Amino acid misincorporation during protein synthesis occurs naturally at a low level. Protein sequence errors, depending on the level and the nature of the misincorporation, can have various consequences. When site-directed mutagenesis is used as a tool for understanding the role of a side chain in enzyme catalysis, misincorporation in a variant with intrinsically low activity may lead to misinterpretations concerning the enzyme mechanism. We report here one more example of such a problem, dealing with flavocytochrome b2 (Fcb2), a lactate dehydrogenase, member of a family of FMN-dependent L-2-hydroxy acid oxidizing enzymes...
March 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/27921457/impact-of-exercise-on-fecal-and-cecal-metabolome-over-aging-a-longitudinal-study-in-rats
#6
Olga Deda, Helen Gika, Theodoros Panagoulis, Ioannis Taitzoglou, Nikolaos Raikos, Georgios Theodoridis
AIM: Physical exercise can reduce adverse conditions during aging, while both exercise and aging act as metabolism modifiers. The present study investigates rat fecal and cecal metabolome alterations derived from exercise during rats' lifespan. METHODS & RESULTS: Groups of rats trained life-long or for a specific period of time were under study. The training protocol consisted of swimming, 15-18 min per day, 3-5 days per week, with load of 4-0% of rat's weight. Fecal samples and cecal extracts were analyzed by targeted and untargeted metabolic profiling methods (GC-MS and LC-MS/MS)...
January 2017: Bioanalysis
https://www.readbyqxmd.com/read/27871307/current-models-of-care-for-disorders-of-sex-development-results-from-an-international-survey-of-specialist-centres
#7
Andreas Kyriakou, Arianne Dessens, Jillian Bryce, Violeta Iotova, Anders Juul, Maciej Krawczynski, Agneta Nordenskjöld, Marta Rozas, Caroline Sanders, Olaf Hiort, S Faisal Ahmed
BACKGROUND: To explore the current models of practice in centres delivering specialist care for children with disorders of sex development (DSD), an international survey of 124 clinicians, identified through DSDnet and the I-DSD Registry, was performed in the last quarter of 2014. RESULTS: A total of 78 (63 %) clinicians, in 75 centres, from 38 countries responded to the survey. A formal national network for managing DSD was reported to exist in 12 (32 %) countries...
November 21, 2016: Orphanet Journal of Rare Diseases
https://www.readbyqxmd.com/read/27691968/effects-of-exercise-on-the-plasma-lipid-profile-in-hispaniolan-amazon-parrots-amazona-ventralis-with-naturally-occurring-hypercholesterolemia
#8
Kate A Gustavsen, Kimber L Stanhope, Amy S Lin, James L Graham, Peter J Havel, Joanne R Paul-Murphy
Hypercholesterolemia is common in psittacines, and Amazon parrots ( Amazona spp.) are particularly susceptible. Associations have been demonstrated between naturally occurring and experimentally induced hypercholesterolemia and atherosclerosis in psittacines. Daily exercise improves lipid metabolism in humans and other mammals, as well as pigeons and chickens, under varying experimental conditions. Hispaniolan Amazon parrots ( Amazona ventralis ) with naturally occurring hypercholesterolemia (343-576 mg/dl) were divided into two groups...
September 2016: Journal of Zoo and Wildlife Medicine: Official Publication of the American Association of Zoo Veterinarians
https://www.readbyqxmd.com/read/27687080/natural-selection-the-evolution-of-diagnostic-criteria-for-gestational-diabetes
#9
REVIEW
Claire L Meek
Gestational diabetes is a common pregnancy disorder which is generally managed with diet, exercise, metformin or insulin treatment and which usually resolves after delivery of the infant. Identifying and treating gestational diabetes improves maternal and fetal outcomes and allows for health promotion to reduce the mother's risk of type 2 diabetes in later life. However, there remains considerable controversy about the optimal method of identification and diagnosis of women with gestational diabetes. The NICE-2015 diagnostic criteria (75 g oral glucose tolerance test (OGTT) 0 h ≥5...
January 2017: Annals of Clinical Biochemistry
https://www.readbyqxmd.com/read/27677251/discovery-of-escherichia-coli-crispr-sequences-in-an-undergraduate-laboratory
#10
Kevin T Militello, Justine C Lazatin
Clustered regularly interspaced short palindromic repeats (CRISPRs) represent a novel type of adaptive immune system found in eubacteria and archaebacteria. CRISPRs have recently generated a lot of attention due to their unique ability to catalog foreign nucleic acids, their ability to destroy foreign nucleic acids in a mechanism that shares some similarity to RNA interference, and the ability to utilize reconstituted CRISPR systems for genome editing in numerous organisms. In order to introduce CRISPR biology into an undergraduate upper-level laboratory, a five-week set of exercises was designed to allow students to examine the CRISPR status of uncharacterized Escherichia coli strains and to allow the discovery of new repeats and spacers...
September 28, 2016: Biochemistry and Molecular Biology Education
https://www.readbyqxmd.com/read/27666582/introducing-the-concept-of-biocatalysis-in-the-classroom-the-conversion-of-cholesterol-to-provitamin-d3
#11
Belén M De Luca, Clara B Nudel, Rodrigo H Gonzalez, Alejandro D Nusblat
Biocatalysis is a fundamental concept in biotechnology. The topic integrates knowledge of several disciplines; therefore, it was included in the course "design and optimization of biological systems" which is offered in the biochemistry curricula. We selected the ciliate tetrahymena as an example of a eukaryotic system with potential for the biotransformation of sterol metabolites of industrial interest; in particular, we focused on the conversion of cholesterol to provitamin D3. The students work with wild type and recombinant strains and learn how sterol pathways could be modified to obtain diverse sterol moieties...
September 26, 2016: Biochemistry and Molecular Biology Education
https://www.readbyqxmd.com/read/27537100/enhancing-student-retention-of-prerequisite-knowledge-through-pre-class-activities-and-in-class-reinforcement
#12
Ann T S Taylor, Eric L Olofson, Walter R P Novak
To foster the connection between biochemistry and the supporting prerequisite concepts, a collection of activities that explicitly link general and organic chemistry concepts to biochemistry ideas was written and either assigned as pre-class work or as recitation activities. We assessed student learning gains after using these activities alone, or in combination with regularly-integrated clicker and discussion questions. Learning gains were determined from student performance on pre- and post-tests covering key prerequisite concepts, biochemistry course exams, and student self-evaluation...
August 18, 2016: Biochemistry and Molecular Biology Education
https://www.readbyqxmd.com/read/27520837/the-12th-international-biochemistry-of-exercise-conference-maastricht-the-netherlands-13-16-july-2003
#13
(no author information available yet)
No abstract text is available yet for this article.
May 2003: European Journal of Applied Physiology
https://www.readbyqxmd.com/read/27496187/pilot-study-of-safety-and-efficacy-of-polyprenols-in-combination-with-coenzyme-q10-in-patients-with-statin-induced-myopathy
#14
Gustavs Latkovskis, Vita Saripo, Emma Sokolova, Dana Upite, Ilona Vanaga, Ugis Kletnieks, Andrejs Erglis
BACKGROUND AND OBJECTIVE: Statin-induced myopathy (SIM) has been partially attributed to deficiency of dolichol and coenzyme Q10 (CoQ10). We aimed to test the safety and efficacy of plant polyprenols in combination with CoQ10 for alleviation of SIM. MATERIALS AND METHODS: In an open-label, one-center prospective pilot study patients with SIM received conifer-tree needle polyprenols (4mg/day) and CoQ10 (100mg/day) for 8 weeks. Symptoms and safety were evaluated according to symptom severity score (0-10), creatine kinase (CK) levels, exercise test, dynamometry, complete blood count, clinical biochemistry and electrocardiography...
2016: Medicina
https://www.readbyqxmd.com/read/27487746/strength-training-prevents-hyperinsulinemia-insulin-resistance-and-inflammation-independent-of-weight-loss-in-fructose-fed-animals
#15
José D Botezelli, Andressa Coope, Ana C Ghezzi, Lucieli T Cambri, Leandro P Moura, Pedro P M Scariot, Rodrigo Stellzer Gaspar, Rania A Mekary, Eduardo Rochete Ropelle, José Rodrigo Pauli
The aim of this study was to compare the effects of aerobic, strength, and combined training on metabolic disorders induced by a fructose-rich diet. Wistar rats (120 days old) were randomized into five groups (n = 8-14): C (control diet and sedentary), F (fed the fructose-rich diet and sedentary), FA (fed the fructose-rich diet and subject to aerobic exercise), FS (fed the fructose-rich diet and subject to strength exercise), and FAS (fed the fructose-rich diet and subject to combined aerobic and strength exercises)...
2016: Scientific Reports
https://www.readbyqxmd.com/read/27459862/impact-of-endurance-exercise-training-in-the-fasted-state-on-muscle-biochemistry-and-metabolism-in-healthy-subjects-can-these-effects-be-of-particular-clinical-benefit-to-type%C3%A2-2-diabetes-mellitus-and-insulin-resistant-patients
#16
REVIEW
Dominique Hansen, Dorien De Strijcker, Patrick Calders
Exercise training intervention is a cornerstone in the care of type 2 diabetes mellitus (T2DM) and insulin resistance (IR), and it is pursued in order to optimize exercise interventions for these patients. In this regard, the nutritional state of patients during exercise (being in the fed or fasted state) can be of particular interest. The aim of the present review is to describe the impact of endurance exercise (training) in the fasted versus fed state on parameters of muscle biochemistry and metabolism linked to glycemic control or insulin sensitivity in healthy subjects...
July 26, 2016: Sports Medicine
https://www.readbyqxmd.com/read/27403640/functional-complementation-analysis-fca-a-laboratory-exercise-designed-and-implemented-to-supplement-the-teaching-of-biochemical-pathways
#17
André O Hudson, Taylor C M Harkness, Michael A Savka
Functional complementation assay (FCA) is an in vivo assay that is widely used to elucidate the function/role of genes/enzymes. This technique is very common in biochemistry, genetics and many other disciplines. A comprehensive overview of the technique to supplement the teaching of biochemical pathways pertaining to amino acids, peptidoglycan and the bacterial stringent response is reported in this manuscript. Two cDNAs from the model plant organism Arabidopsis thaliana that are involved in the metabolism of lysine (L,L-diaminopimelate aminotransferase (dapL) and tyrosine aminotransferase (tyrB) involved in the metabolism of tyrosine and phenylalanine are highlighted...
2016: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/27377256/feasibility-of-higher-intensity-exercise-in-patients-with-chronic-kidney-disease
#18
Kassia S Beetham, Erin J Howden, Rathika Krishnasamy, Nicole M Isbel, Jeff S Coombes
BACKGROUND: Higher intensity exercise is a more effective way of evoking improvements in cardiorespiratory fitness in many chronic disease populations compared to moderate intensity continuous training. The aim of this study was to investigate the feasibility of participation in higher intensity exercise in patients with chronic kidney disease (CKD). METHODS: This study is an observational sub-study of the intervention arm of a randomized control trial. Forty-four participants with stage 3-4 CKD in the intervention arm were required to complete a predominantly home-based 12 month individualized exercise intervention...
July 5, 2016: Journal of Sports Medicine and Physical Fitness
https://www.readbyqxmd.com/read/27353527/arsenic-based-life-an-active-learning-assignment-for-teaching-scientific-discourse
#19
R Jeremy Johnson
Among recent high profile scientific debates was the proposal that life could exist with arsenic in place of phosphorous in its nucleic acids and other biomolecules. Soon after its initial publication, scientists across diverse disciplines began to question this extraordinary claim. Using the original article, its claims, its scientific support, and the ensuing counterarguments, a two-day, active learning classroom exercise was developed focusing on the presentation, evaluation, and discussion of scientific argumentation and discourse...
January 2, 2017: Biochemistry and Molecular Biology Education
https://www.readbyqxmd.com/read/27241834/using-the-pymol-application-to-reinforce-visual-understanding-of-protein-structure
#20
Rachel E Rigsby, Alison B Parker
Visualization of chemical concepts can be challenging for many students. This is arguably a critical skill for beginning students of biochemistry to develop, since new information is often presented visually in the form of textbook figures. It is recommended that visual literacy be explicitly taught in the classroom rather than assuming that students will develop this skill on their own.  The activity described here is designed to assist students in their development of understanding of basic representations of protein three-dimensional structure as well as various types of ligands (small molecules, ions) through the use of the iPad application PyMOL...
September 10, 2016: Biochemistry and Molecular Biology Education
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