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https://www.readbyqxmd.com/read/28597434/ectopic-expression-of-lc-differentially-regulated-anthocyanin-biosynthesis-in-the-floral-parts-of-tobacco-nicotiana-tobacum-l-plants
#1
Zong-An Huang, Ting Zhao, Ning Wang, Shu-Song Zheng
BACKGROUND: Anthocyanins are the conspicuous pigments of flowering plants and participate in several aspects of plant development and defense, such as seeds and pollens dispersal. Leaf colour (Lc) is the first basic/helix-loop-helix (bHLH) transcription factor controlling anthocyanin biosynthesis isolated from maize (Zea mays L.). Ectopic expression of maize Lc enhanced anthocyanin biosynthesis in many plants including tobacco (Nicotiana tobacum L.). However, the molecular regulatory mechanism of anthocyanin biosynthesis in the different floral parts of tobacco remains largely unknown...
December 2016: Botanical Studies (Taipei, Taiwan)
https://www.readbyqxmd.com/read/28573233/geno-protective-and-anti-apoptotic-effect-of-green-tea-against-perinatal-lipopolysaccharide-exposure-induced-liver-toxicity-in-rat-newborns
#2
Ahmed A Allam, Sami A Gabr, Jamaan Ajarem, Ahmad H Alghadir, Revathi Sekar, Billy Kc Chow
BACKGROUND: This study aims to examine the protective effect of green tea on the disturbances in oxidative stress and apoptosis related factors, mostly produced due to perinatal lipopolysaccharide (LPS) exposure, that subsequently induces liver cell damage. MATERIALS AND METHODS: Anti-free radical, Antioxidant, scavenging, geno-protective, and antiapoptotic activity of aqueous green tea extract (AGTE) were assessed against LPS-induced hepatic dysfunction in newborn-rats...
2017: African Journal of Traditional, Complementary, and Alternative Medicines: AJTCAM
https://www.readbyqxmd.com/read/28405813/estimation-of-dietary-total-antioxidant-capacity-of-korean-adults
#3
Shinyoung Jun, Ock K Chun, Hyojee Joung
PURPOSE: Dietary total antioxidant capacity (TAC) has been reported to be inversely associated with the risk of chronic diseases. However, little is known about dietary TAC among the Korean population. Therefore, we aimed to estimate the dietary TAC of Korean adults and to document the major food sources. METHODS: We studied 33,581 Korean adults aged ≥19 years who participated in the Korea National Health and Nutrition Examination Survey 2007-2012. Dietary TAC was estimated from 1-day 24-h dietary recall data using a TAC database comprising antioxidant capacities of 42 dietary antioxidants, developed for common Korean foods...
April 12, 2017: European Journal of Nutrition
https://www.readbyqxmd.com/read/28329737/relative-strengths-and-regulation-of-different-promoter-associated-sequences-for-various-blashv-genes-and-their-relationships-to-%C3%AE-lactam-resistance
#4
Yao Zhai, Zhao Zhang, Zhanwei Wang, Yusheng Chen, Qi Wang, Yuan Lv, Jingping Yang, Tong Zhao, Yatao Guo, Zhancheng Gao
AIMS: This work investigated the relative strengths of different blaSHV promoter-associated sequences and their regulation function in blaSHV expression and β-lactam resistance. METHODS: Recombinant plasmids with the promoter-associated sequences (P-W, P-S, P-IS, and P-WPD), tac promoter, and combined fragments of promoter and blaSHV were separately constructed and transformed into Escherichia coli DH5α. The relative strengths of the promoters indicated by the intensities of green fluorescent protein and the mRNA expression levels of blaSHV were compared...
2017: Journal of Molecular Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28314435/effect-of-green-tea-camellia-sinensis-extract-and-pre-freezing-equilibration-time-on-the-post-thawing-quality-of-ram-semen-cryopreserved-in-a-soybean-lecithin-based-extender
#5
Mahdieh Mehdipour, Hossein Daghigh Kia, Abouzar Najafi, Hossein Vaseghi Dodaran, Olga García-Álvarez
The aim of this study was to determine the effect of Camellia sinensis extract as antioxidant supplement and pre-freezing equilibration times in a soybean lecithin extender for freezing ram semen. In this study, a total of 20 ejaculates were collected from four Ghezel rams and diluted with extenders (1.5% soybean lecithin, 7% glycerol) containing no supplements (control) and Camellia sinensis extract (5, 10, and 15 mg/L) and cryopreserved, immediately after thermal equilibrium was reached at 5 °C (0 h), or 4 h after equilibration...
December 2016: Cryobiology
https://www.readbyqxmd.com/read/28259635/characterization-of-the-cryptic-plasmid-pofk55-from-legionella-pneumophila-and-construction-of-a-pofk55-derived-shuttle-vector
#6
Takashi Nishida, Kenta Watanabe, Masato Tachibana, Takashi Shimizu, Masahisa Watarai
In this study, a cryptic plasmid pOfk55 from Legionella pneumophila was isolated and characterized. pOfk55 comprised 2584bp with a GC content of 37.3% and contained three putative open reading frames (ORFs). orf1 encoded a protein of 195 amino acids and the putative protein shared 39% sequence identity with a putative plasmid replication protein RepL. ORF1 was needed for replication in L. pneumophila but pOfk55 did not replicate in Escherichia coli. orf2 and orf3 encoded putative hypothetical proteins of 114 amino acids and 78 amino acids, respectively, but the functions of the putative proteins ORF2 and OFR3 are not clear...
March 2017: Plasmid
https://www.readbyqxmd.com/read/27983946/effect-of-green-tea-camellia-sinensis-extract-and-pre-freezing-equilibration-time-on-the-post-thawing-quality-of-ram-semen-cryopreserved-in-a-soybean-lecithin-based-extender
#7
Mahdieh Mehdipour, Hossein Daghigh Kia, Abouzar Najafi, Hossein Vaseghi Dodaran, Olga García-Álvarez
The aim of this study was to determine the effect of Camellia sinensis extract as antioxidant supplement and pre-freezing equilibration times in a soybean lecithin extender for freezing ram semen. In this study, a total of 20 ejaculates were collected from four Ghezel rams and diluted with extenders (1.5% soybean lecithin, 7% glycerol) containing no supplements (control) and Camellia sinensis extract (5, 10, and 15 mg/L) and cryopreserved, immediately after thermal equilibrium was reached at 5 °C (0 h), or 4 h after equilibration...
October 28, 2016: Cryobiology
https://www.readbyqxmd.com/read/27862403/egg-production-performance-egg-yolk-antioxidant-profile-and-excreta-concentration-of-corticosterone-in-golden-pheasants-chrysolophus-pictus-fed-diets-containing-different-levels-of-green-vegetables
#8
S S Kullu, A Das, S K Bajpai, A K Garg, R K Yogi, M Saini, A K Sharma
In order to study the effect of feeding graded levels of green vegetables on egg production performance and egg yolk antioxidant status, 27 female golden pheasants (GP) were randomly distributed into three groups of nine each in an experiment based on completely randomized design. The diets of the birds in groups T1 , T2 and T3 contained 1.4%, 2.7% and 5.0% of green vegetables respectively. Feeding of experimental diets started on 12th February (day 1) and was continued till 30 June 2012. Average number of eggs laid and egg mass produced by the hens in group T3 was higher (p < 0...
November 9, 2016: Journal of Animal Physiology and Animal Nutrition
https://www.readbyqxmd.com/read/27736246/the-effect-of-green-tea-and-sour-tea-hibiscus-sabdariffa-l-supplementation-on-oxidative-stress-and-muscle-damage-in-athletes
#9
RANDOMIZED CONTROLLED TRIAL
Amir Hadi, Makan Pourmasoumi, Marzieh Kafeshani, Jahangir Karimian, Mohammad Reza Maracy, Mohammad Hasan Entezari
OBJECTIVE: Additional oxygen consumption during intense exercises may lead to oxidative stress and contribute to muscular fatigue. Green tea and sour tea (Hibiscus sabdariffa L.), which contain various flavonoids and polyphenols, have many healthful properties such as anticarcinogenic, anti-inflammatory, and heart protecting effects. The aim of the present study was to assess the effects of green tea and sour tea supplementation on oxidative stress and muscle damage in soccer athletes...
May 4, 2017: Journal of Dietary Supplements
https://www.readbyqxmd.com/read/27698166/validation-of-three-automated-assays-for-total-antioxidant-capacity-determination-in-canine-serum-samples
#10
Camila Peres Rubio, Josefa Hernández-Ruiz, Silvia Martinez-Subiela, Asta Tvarijonaviciute, Marino Bañón Arnao, José Joaquín Ceron
We performed analytical validation of 3 automated assays of the total antioxidant capacity (TAC) in canine serum and evaluated their use in dogs with inflammatory bowel disease. The assays were based on the generation of a 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) radical cation (ABTS•(+)) in aqueous media, which produces a blue-green color. The antioxidants present in the sample remove the chromogen in proportion to their concentrations. The assays differed mainly in the way in which this radical was produced...
November 2016: Journal of Veterinary Diagnostic Investigation
https://www.readbyqxmd.com/read/27080884/colored-light-quality-selective-plastic-films-affect-anthocyanin-content-enzyme-activities-and-the-expression-of-flavonoid-genes-in-strawberry-fragaria-%C3%A3-ananassa-fruit
#11
Lixiang Miao, Yuchao Zhang, Xiaofang Yang, Jinping Xiao, Huiqin Zhang, Zuofa Zhang, Yuezhi Wang, Guihua Jiang
The influence of colored light-quality selective plastic films (red, yellow, green, blue, and white) on the content of anthocyanin, the activities of the related enzymes and the transcripts of the flavonoid gene was studied in developing strawberry fruit. The results indicated that colored films had highly significant effects on the total anthocyanin content (TAC) and proportions of individual anthocyanins. Compared with the white control film, the red and yellow films led to the significant increase of TAC, while the green and blue films caused a decrease of TAC...
September 15, 2016: Food Chemistry
https://www.readbyqxmd.com/read/26939442/-overexpressing-3-ketosteroid-%C3%AE-1-dehydrogenase-for-degrading-phytosterols-into-androst-1-4-diene-3-17-dione
#12
Lele Zhang, Xian Zhang, Minglong Shao, Rongrong Chen, Zhiming Rao, Hu Li, Zhenghong Xu
We constructed plasmid pMTac to overexpress 3-ketosteroid-Δ1-dehydrogenase (KSDD) in Mycobacterium neoaurum JC-12 for improving androst-1,4-diene-3,17-dione (ADD) production. To construct pMTac, pACE promoter on pMF41 was replaced by tac promoter, and then four recombinants were constructed, which were M. neoaurum JC-12/pMF41-gfp, M. neoaurum JC-12/pMTac-gfp, M. neoaurum JC-12/pMF41-ksdd and M. neoaurum JC-12/pMTac-ksdd. Fluorescence detection results show that much more green fluorescent protein (GFP) was expressed in M...
November 2015: Sheng Wu Gong Cheng Xue Bao, Chinese Journal of Biotechnology
https://www.readbyqxmd.com/read/26854847/pharmacokinetics-and-plasma-cellular-antioxidative-effects-of-flavanols-after-oral-intake-of-green-tea-formulated-with-vitamin-c-and-xylitol-in-healthy-subjects
#13
Yu-Ra Son, Tae-Sik Park, Soon-Mi Shim
This study aimed to test whether green tea formulated with vitamin C and xylitol (GTVX) could improve absorption of flavanols and total antioxidant activity (TAC) of plasma compared with green tea only (GT) in healthy subjects. The total radical-trapping antioxidant parameter method was used to measure the TAC of plasma. Cmax, Tmax, and area under the curve (AUC) of flavanols in plasma after consumption of GTVX were 5980.58 μg/mL, 2.14 h, and 18,915.56 h·μg/mL, respectively, indicating that GTVX showed significantly higher AUC than GT (13,855...
February 2016: Journal of Medicinal Food
https://www.readbyqxmd.com/read/26784463/a-comparison-of-total-antioxidant-capacities-of-concord-purple-red-and-green-grapes-using-the-cuprac-assay
#14
Connor M Callaghan, Robert E Leggett, Robert M Levin
Considering how popular grapes are in terms of their antioxidant benefits, we compared concord, purple, red, and green grapes for total antioxidant capacity (TAC) and carbohydrate concentration. All grapes were acquired from commercial sources and samples of each were separated into skinned and not skinned groups. Each whole grape and the skins were individually homogenized and then separated into pulp and supernatant fractions. Each fraction was analyzed for total TAC and carbohydrates. The concord grapes and purple grapes had significantly higher TAC in the homogenates than did the red or green grapes...
2013: Antioxidants (Basel, Switzerland)
https://www.readbyqxmd.com/read/26784461/antioxidant-and-antiradical-activity-of-coffee
#15
REVIEW
Alexander Yashin, Yakov Yashin, Jing Yuan Wang, Boris Nemzer
This review summarizes published information concerning the determination of antioxidant activity (AA) in coffee samples by various methods (ORAC, FRAP, TRAP, TEAC, etc.) in vitro and limited data of antiradical activity of coffee products in vitro and in vivo. Comparison is carried out of the AA of coffee Arabica and coffee Robusta roasted at different temperatures as well as by different roasting methods (microwave, convection, etc.). Data on the antiradical activity of coffee is provided. The antioxidant activity of coffee, tea, cocoa, and red wine is compared...
2013: Antioxidants (Basel, Switzerland)
https://www.readbyqxmd.com/read/26688290/light-induced-biochemical-variations-in-secondary-metabolite-production-and-antioxidant-activity-in-callus-cultures-of-stevia-rebaudiana-bert
#16
Naveed Ahmad, Abdur Rab, Nisar Ahmad
Stevia rebaudiana (S. rebaudiana) is a very important species with worldwide medicinal and commercial uses. Light is one of the major elicitors that fluctuate morphogenic potential and biochemical responses. In the present study, we investigated the effect of various spectral lights on biomass accumulation and secondary metabolite production in callus cultures of S. rebaudiana. Leaf explants were placed on Murashige and Skoog (MS) medium and exposed to various spectral lights. 6-Benzyle adenine (BA) and 2, 4-dichlorophenoxy acetic acid (2, 4-D; 2...
January 2016: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/26587804/c-kit-resident-cardiac-stem-cells-improve-left-ventricular-fibrosis-in-pressure-overload
#17
Andrey Kazakov, Timo Meier, Christian Werner, Rabea Hall, Birgit Klemmer, Christina Körbel, Frank Lammert, Christoph Maack, Michael Böhm, Ulrich Laufs
To investigate the effect of resident cardiac stem cells (RCSC) on myocardial remodeling, c-kit(+) RCSC were isolated from hearts of C57Bl/6-Tg (ACTb-EGFP)1Osb/J mice expressing green fluorescent protein and expanded in vitro. C57/Bl6N wildtype mice were subjected to transverse aortic constriction (TAC, 360 μm) or sham-operation. 5 × 10(5) c-kit(+) RCSC or c-kit(-) cardiac cells or cell buffer were infused intravenously 24 h post-surgery (n = 11-24 per group). Hypoxia-inducible factor-1α-mRNA in left ventricles of TAC mice was enhanced 24 h after transplantation...
November 2015: Stem Cell Research
https://www.readbyqxmd.com/read/26572874/an-apple-plus-a-brazil-nut-a-day-keeps-the-doctors-away-antioxidant-capacity-of-foods-and-their-health-benefits
#18
REVIEW
Carlos Kusano Bucalen Ferrari, Sandro Percário, José Carlos Costa Baptista Silva, Elizabeth Aparecida Ferraz da Silva Torres
Antioxidant-rich foods scavenge free radicals and other reactive species, decreasing the risk of different non-communicable chronic diseases. The objective of this study was to review the content of total antioxidant capacity of commonly foods comparing with experimental data and to explore the health benefits due to foods with moderate to high TAC. The TAC was analytically measured using the "Total Antioxidant Capacity" (NX2332) test from Randox® (UK) by spectrometry at 600 nm. Brazil nut (Bertholletia excelsa), "guaraná" (Paullinia cupana Kunth) powder, ready to drink boiled coffee (Coffea arabica L...
2016: Current Pharmaceutical Design
https://www.readbyqxmd.com/read/26503400/oligonucleotide-directed-mutagenesis-for-precision-gene-editing
#19
REVIEW
Noel J Sauer, Jerry Mozoruk, Ryan B Miller, Zachary J Warburg, Keith A Walker, Peter R Beetham, Christian R Schöpke, Greg F W Gocal
Differences in gene sequences, many of which are single nucleotide polymorphisms, underlie some of the most important traits in plants. With humanity facing significant challenges to increase global agricultural productivity, there is an urgent need to accelerate the development of these traits in plants. oligonucleotide-directed mutagenesis (ODM), one of the many tools of Cibus' Rapid Trait Development System (RTDS(™) ) technology, offers a rapid, precise and non-transgenic breeding alternative for trait improvement in agriculture to address this urgent need...
February 2016: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/26345170/association-of-blood-antioxidants-status-with-visual-and-auditory-sustained-attention
#20
Farideh Shiraseb, Fereydoun Siassi, Gity Sotoudeh, Mostafa Qorbani, Reza Rostami, Vahid Sadeghi-Firoozabadi, Elham Narmaki
OBJECTIVE: A low antioxidants status has been shown to result in oxidative stress and cognitive impairment. Because antioxidants can protect the nervous system, it is expected that a better blood antioxidant status might be related to sustained attention. However, the relationship between the blood antioxidant status and visual and auditory sustained attention has not been investigated. The aim of this study was to evaluate the association of fruits and vegetables intake and the blood antioxidant status with visual and auditory sustained attention in women...
2015: Nutritional Neuroscience
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