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https://www.readbyqxmd.com/read/29332293/measurement-of-cyclic-gmp-during-plant-hypersensitive-disease-resistance-response
#1
Jian Chen, Diana Bellin, Elodie Vandelle
Cyclic guanosine-3',5'-monophosphate (cGMP) is recognized as an important second messenger in plants, mediating intracellular signal in important physiological processes, including the hypersensitive disease resistance response induced by avirulent pathogens. In this context, the analysis of cGMP levels in infected plants requires an accurate and specific detection method allowing its quantification. Here, we describe an assay based on the Alphascreen technology, developed for animal cells and further adapted and optimized for the detection of cGMP in plants...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29332268/methylmercury-intoxication-promotes-metallothionein-response-and-cell-damage-in-salivary-glands-of-rats
#2
Leidiane Alencar de Oliveira Lima, Leonardo Oliveira Bittencourt, Bruna Puty, Rafael Monteiro Fernandes, Priscila Cunha Nascimento, Marcia Cristina Freitas Silva, Sérgio Melo Alves-Junior, João de Jesus Viana Pinheiro, Rafael Rodrigues Lima
Environmental and occupational mercury exposure is considered a major public health issue. Despite being well known that MeHg exposure causes adverse effects in several physiologic functions, MeHg effects on salivary glands still not completely elucidated. Here, we investigated the cellular MeHg-induced damage in the three major salivary glands (parotid, submandibular, and sublingual) of adult rats after chronic, systemic and low doses of MeHg exposure. Rats were exposed by 0.04 mg/kg/day over 60 days. After that, animals were euthanized and all three glands were collected...
January 14, 2018: Biological Trace Element Research
https://www.readbyqxmd.com/read/29332128/pharmacologic-characterization-of-omidenepag-isopropyl-a-novel-selective-ep2-receptor-agonist-as-an-ocular-hypotensive-agent
#3
Tomoko Kirihara, Takazumi Taniguchi, Kenzo Yamamura, Ryo Iwamura, Kenji Yoneda, Noriko Odani-Kawabata, Atsushi Shimazaki, Takeshi Matsugi, Naveed Shams, Jin-Zhong Zhang
Purpose: The objective of this study was to investigate the pharmacologic characteristics of omidenepag isopropyl (OMDI), a compound developed as a novel intraocular pressure (IOP)-lowering agent, with better IOP control and fewer side effects than other prostanoid receptor agonists such as prostaglandin F receptor (FP) agonists. Methods: Binding activities of OMDI and its hydrolyzed form, omidenepag (OMD), to human recombinant prostanoid receptors (DP1-2, EP1-4, FP, and IP) were evaluated...
January 1, 2018: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/29332081/radiosurgical-subthalamic-nucleotomy
#4
Jean Régis, Romain Carron, Tatiana Witjas
Deep brain stimulation (DBS) of the subthalamic nucleus (STN) is the reference technique in Parkinson's disease (PD) at different stages of complications. Some patients cannot afford DBS due to anticoagulation or comorbidities or due to pecuniary reasons. Radiosurgery is a minimally invasive stereotactic technique, with no craniotomy and subsequently no risk of bleeding or infection. Its good safety efficacy profile has been established in the treatment of tremor, and the postoperative care issues are simple with a much shorter hospital stay (mean 48 h)...
2018: Progress in Neurological Surgery
https://www.readbyqxmd.com/read/29332044/does-the-genetic-feature-of-the-chinese-tree-shrew-tupaia-belangeri-chinensis-support-its-potential-as-a-viable-model-for-alzheimer-s-disease-research
#5
Yu Fan, Rongcan Luo, Ling-Yan Su, Qun Xiang, Dandan Yu, Ling Xu, Jia-Qi Chen, Rui Bi, Dong-Dong Wu, Ping Zheng, Yong-Gang Yao
Alzheimer's disease (AD) is a neurodegenerative disease with increasing incidence across the world and no cure at the present time. An ideal animal model would facilitate the understanding of the pathogenesis of AD and discovery of potential therapeutic targets. The Chinese tree shrew (Tupaia belangeri chinensis) has a closer genetic affinity to primates relative to rodents, and can attain ages of 8 years or older, which represents another advantage for the study of neurodegenerative diseases such as AD compared to primates...
2018: Journal of Alzheimer's Disease: JAD
https://www.readbyqxmd.com/read/29332040/synthetic-fragment-of-receptor-for-advanced-glycation-end-products-prevents-memory-loss-and-protects-brain-neurons-in-olfactory-bulbectomized-mice
#6
Olga M Volpina, Alexandr N Samokhin, Dmitriy O Koroev, Inna V Nesterova, Tatyana D Volkova, Natalia I Medvinskaya, Pavel V Nekrasov, Olga G Tatarnikova, Anna V Kamynina, Samson M Balasanyants, Tamara A Voronina, Alexey M Kulikov, Natalia V Bobkova
Activation of receptor for advanced glycation end products (RAGE) plays an essential role in the development of Alzheimer's disease (AD). It is known that the soluble isoform of the receptor binds to ligands and prevents negative effects of the receptor activation. We proposed that peptide fragments from RAGE prevent negative effects of the receptor activation during AD neurodegeneration. We have synthesized peptide fragments from surface-exposed regions of RAGE. Peptides were intranasally administrated into olfactory bulbectomized (OBX) mice, which developed some characteristics similar to AD neurodegeneration...
2018: Journal of Alzheimer's Disease: JAD
https://www.readbyqxmd.com/read/29332037/increased-vulnerability-of-the-hippocampus-in-transgenic-mice-overexpressing-app-and-triple-repeat-tau
#7
Andrew Arner, Edward Rockenstein, Michael Mante, Jazmin Florio, Deborah Masliah, Bahar Salehi, Anthony Adame, Cassia Overk, Eliezer Masliah, Robert A Rissman
 Alzheimer's disease (AD) is the most common tauopathy, characterized by progressive accumulation of amyloid-β (Aβ) and hyperphosphorylated tau. While pathology associated with the 4-repeat (4R) tau isoform is more abundant in corticobasal degeneration and progressive supranuclear palsy, both 3R and 4R tau isoforms accumulate in AD. Many studies have investigated interactions between Aβ and 4R tau in double transgenic mice, but few, if any, have examined the effects of Aβ with 3R tau. To examine this relationship, we crossed our APP751 mutant line with our recently characterized 3R tau mutant model to create a bigenic line (hAPP-3RTau) to model AD neuropathology...
2018: Journal of Alzheimer's Disease: JAD
https://www.readbyqxmd.com/read/29331959/probenecid-improves-cardiac-function-in-patients-with-heart-failure-with-reduced-ejection-fraction-in-vivo-and-cardiomyocyte-calcium-sensitivity-in-vitro
#8
Nathan Robbins, Mark Gilbert, Mohit Kumar, James W McNamara, Patrick Daly, Sheryl E Koch, Ginger Conway, Mohamed Effat, Jessica G Woo, Sakthivel Sadayappan, Jack Rubinstein
BACKGROUND: Transient receptor potential vanilloid 2 is a calcium channel activated by probenecid. Probenecid is a Food and Drug Administration-approved uricosuric drug that has recently been shown to induce positive lusitropic and inotropic effects in animal models through cardiomyocyte transient receptor potential vanilloid 2 activation. The aim of this study was to test the hypothesis that oral probenecid can improve cardiac function and symptomatology in patients with heart failure with reduced ejection fraction and to further elucidate its calcium-dependent effects on myocyte contractility...
January 13, 2018: Journal of the American Heart Association
https://www.readbyqxmd.com/read/29331932/cnv-biology-in-neurodevelopmental-disorders
#9
REVIEW
Toru Takumi, Kota Tamada
Copy number variants (CNVs), characterized in recent years by cutting-edge technology, add complexity to our knowledge of the human genome. CNVs contribute not only to human diversity but also to different kinds of diseases including neurodevelopmental delay, autism spectrum disorder and neuropsychiatric diseases. Interestingly, many pathogenic CNVs are shared among these diseases. Studies suggest that pathophysiology of disease may not be simply attributed to a single driver gene within a CNV but also that multifactorial effects may be important...
January 10, 2018: Current Opinion in Neurobiology
https://www.readbyqxmd.com/read/29331890/deep-sequence-analysis-reveals-the-ovine-rumen-as-a-reservoir-of-antibiotic-resistance-genes
#10
Thomas C A Hitch, Ben J Thomas, Jessica C A Friedersdorff, Helen Ougham, Christopher J Creevey
Antibiotic resistance is an increasingly important environmental pollutant with direct consequences for human health. Identification of environmental sources of antibiotic resistance genes (ARGs) makes it possible to follow their evolution and prevent their entry into the clinical setting. ARGs have been found in environmental sources exogenous to the original source and previous studies have shown that these genes are capable of being transferred from livestock to humans. Due to the nature of farming and the slaughter of ruminants for food, humans interact with these animals in close proximity, and for this reason it is important to consider the risks to human health...
January 11, 2018: Environmental Pollution
https://www.readbyqxmd.com/read/29331881/ameliorative-role-of-genistein-against-age-dependent-chronic-arsenic-toxicity-in-murine-brains-via-the-regulation-of-oxidative-stress-and-inflammatory-signaling-cascades
#11
Sukanya Saha, Pritam Sadhukhan, Sushweta Mahalanobish, Sayanta Dutta, Parames C Sil
Brain is highly prone to oxidative damage due to its huge lipid content and extensive energy requirements. Exogenous insult in brain via oxidative injury can lead to severe pathophysiological conditions. Age-dependent deterioration of normal brain functions is also noteworthy. Genistein, a polyphenolic isoflavonoid, obtained from the soy plant, is well known to protect against several diseased conditions. Here, in this study chronic brain toxicity model was developed using oral administration of arsenic for 90 days in adult and aged murines...
December 12, 2017: Journal of Nutritional Biochemistry
https://www.readbyqxmd.com/read/29331868/zinc-supplementation-alleviates-the-progression-of-diabetic-nephropathy-by-inhibiting-the-overexpression-of-oxidative-stress-mediated-molecular-markers-in-streptozotocin-induced-experimental-rats
#12
Susmita Barman, Seetur R Pradeep, Krishnapura Srinivasan
Zinc deficiency during diabetes projects a role for zinc nutrition in the management of diabetic nephropathy. The current study explored whether zinc supplementation protects against diabetic nephropathy through modulation of kidney oxidative stress and stress-induced expression related to the inflammatory process in streptozotocin-induced diabetic rats. Groups of hyperglycemic rats were exposed to dietary interventions for 6 weeks with zinc supplementation (5 times and 10 times the normal level). Supplemental-zinc-fed diabetic groups showed a significant reversal of increased kidney weight and creatinine clearance...
December 11, 2017: Journal of Nutritional Biochemistry
https://www.readbyqxmd.com/read/29331853/mobility-of-heavy-metals-in-sandy-soil-after-application-of-composts-produced-from-maize-straw-sewage-sludge-and-biochar
#13
K Gondek, M Mierzwa-Hersztek, M Kopeć
Studies on the availability of heavy metals in composted organic materials and in soil amended with these materials are of practical significance. They are used in the assessment of the purity of the soil environment and of the biological value of plants intended for human and animal consumption. Composting of organic materials has a significant effect on changes in mobile forms of heavy metals. Therefore, the aim of this study was to determine the effect of the addition of biochar and sewage sludge on (i) the contents of water soluble forms of Cu, Cd, Pb, and Zn in composts; and (ii) the contents of mobile forms of these elements in sandy soil after the addition of composts...
January 11, 2018: Journal of Environmental Management
https://www.readbyqxmd.com/read/29331851/rhizospheric-microorganisms-as-a-solution-for-the-recovery-of-soils-contaminated-by-petroleum-a-review
#14
REVIEW
Jéssica Janzen Dos Santos, Leila Teresinha Maranho
Petroleum is currently the world's main energy source, and its demand is expected to increase in coming years. Its intense exploitation can lead to an increase in the number of environmental accidents, such as spills and leaks, and an increase in the generation of environmental liabilities resulting from refining. Due to its hydrophobic characteristics and slow process of biodegradation, petroleum can remain in the environment for a long time and its toxicity can cause a negative impact on both terrestrial and aquatic ecosystems, with the main negative effects related to its carcinogenic potential for both animals and humans...
January 11, 2018: Journal of Environmental Management
https://www.readbyqxmd.com/read/29331830/fetal-development-of-the-poeppig-s-woolly-monkey-lagothrix-poeppigii
#15
Rafael Dos Santos de Andrade, Frederico Ozanan Barros Monteiro, Hani Rocha El Bizri, Wilter Ricardo Russiano Vicente, Diva Anelie de Araujo Guimarães, Pedro Mayor
The intrauterine development is an evolutionary strategy that prepares the neonate for extra-uterine life, thus providing important information on the life history of species. In this study, we described the external and internal morphology of 25 fetuses of Poeppig's woolly monkeys (Lagothrix poeppigii) by taking advantage of a 10-year participatory collection of biological samples originated from animals hunted for subsistence purposes in the Peruvian Amazon. Logistic regressions estimated the probability of occurrence of each external morphological characteristic in relation to the crown-rump length (CRL)...
December 8, 2017: Theriogenology
https://www.readbyqxmd.com/read/29331808/simultaneous-inhibition-of-hedgehog-signaling-and-tumor-proliferation-remodels-stroma-and-enhances-pancreatic-cancer-therapy
#16
Jun Zhao, Huamin Wang, Cheng-Hui Hsiao, Diana S-L Chow, Eugene J Koay, Yaan Kang, Xiaoxia Wen, Qian Huang, Ying Ma, James A Bankson, Stephen E Ullrich, Willem Overwijk, Anirban Maitra, David Piwnica-Worms, Jason B Fleming, Chun Li
Pancreatic ductal adenocarcinoma (PDAC) is one of the deadliest cancers. It has an excessive desmoplastic stroma that can limit the intratumoral delivery of chemotherapy drugs, and protect tumor cells against radiotherapy. Therefore, both stromal and tumor compartments need to be addressed in order to effectively treat PDAC. We hereby co-deliver a sonic hedgehog inhibitor, cyclopamine (CPA), and a cytotoxic chemotherapy drug paclitaxel (PTX) with a polymeric micelle formulation (M-CPA/PTX). CPA can deplete the stroma-producing cancer-associated fibroblasts (CAFs), while PTX can inhibit tumor proliferation...
January 9, 2018: Biomaterials
https://www.readbyqxmd.com/read/29331789/anticonvulsant-activities-of-%C3%AE-asaronol-e-3-hydroxyasarone-an-active-constituent-derived-from-%C3%AE-asarone
#17
Xirui He, Yajun Bai, Min Zeng, Zefeng Zhao, Qiang Zhang, Ning Xu, Fanggang Qin, Xiaoyang Wei, Meimei Zhao, Ni Wu, Zehua Li, Yajun Zhang, Tai-Ping Fan, Xiaohui Zheng
BACKGROUND: Epilepsy is one of chronic neurological disorders that affects 0.5-1.0% of the world's population during their lifetime. There is a still significant need to develop novel anticonvulsant drugs that possess superior efficacy, broad spectrum of activities and good safety profile. METHODS: α-Asaronol and two current antiseizure drugs (α-asarone and carbamazepine (CBZ)) were assessed by in vivo anticonvulsant screening with the three most employed standard animal seizure models, including maximal electroshock seizure (MES), subcutaneous injection-pentylenetetrazole (PTZ)-induced seizures and 3-mercaptopropionic acid (3-MP)-induced seizures in mice...
August 31, 2017: Pharmacological Reports: PR
https://www.readbyqxmd.com/read/29331788/curcumin-inhibits-cardiac-hypertrophy-and-improves-cardiovascular-function-via-enhanced-na-ca2-exchanger-expression-after-transverse-abdominal-aortic-constriction-in-rats
#18
Xiao-Jie Bai, Jun-Tao Hao, Jin Wang, Wei-Fang Zhang, Cai-Ping Yan, Jia-Hui Zhao, Zhi-Qing Zhao
BACKGROUND: This study tested the hypothesis that inhibition of cardiac hypertrophy and preservation of cardiac/endothelial function by the natural yellow pigment curcumin are associated with upregulated expression of Na+/Ca2+ exchanger (NCX) after transverse aortic constriction (TAC). METHODS: Male Wistar rats were subjected to TAC for 10 weeks and curcumin (50 mg/kg/day) was fed by gastric gavage during TAC. Expression of NCX and endothelial nitric oxide synthase (eNOS) was analyzed by Western blot and immunohistochemistry...
July 15, 2017: Pharmacological Reports: PR
https://www.readbyqxmd.com/read/29331765/5-6-dihydropyrimidine-1-2h-carbothioamides-synthesis-in-vitro-gaba-at-screening-anticonvulsant-activity-and-molecular-modelling-study
#19
Meeta Sahu, Nadeem Siddiqui, Vidushi Sharma, Sharad Wakode
Even after considerable advances in the field of epilepsy treatment, convulsions are inefficiently controlled by standard drug therapy. Herein, a series of pyrimidine-carbothioamide derivatives 4(a-t) was designed as anticonvulsant agents by doing some important structural modifications in well-known anticonvulsant drugs. Two classical animal models were used for the in vivo anticonvulsant screening, maximum electroshock seizure (MES) and subcutaneous pentylenetetrazole (scPTZ) models; followed by motor impairment study by rotarod method...
January 2, 2018: Bioorganic Chemistry
https://www.readbyqxmd.com/read/29331742/a-sensitive-and-selective-immunoaffinity-column-clean-up-coupled-to-uplc-ms-ms-for-determination-of-trace-methyl-3-quinoxaline-2-carboxylic-acid-in-animal-tissues
#20
Peipei Li, Xiaojun Zhang, Juanmei Zhang, Zhongyong Yan, Shuai Zhang, Si Chen, Yi Fang
This paper described a reliable and simple method for the selective determination of MQCA in animal tissues using ultra high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS). A highly targeted immunoaffinity column was used for sample purification after enzymatic hydrolysis. The purified extracts were analyzed by reversed-phase HPLC-MS/MS in positive ESI and multiple reaction monitoring mode. The calibration curves showed good linearity with correlation coefficient (r2) larger than 0...
January 3, 2018: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
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