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https://www.readbyqxmd.com/read/28931104/-molecular-diagnostics-of-alk-positive-lung-cancer
#1
József Tímár, Gábor Lotz, Erzsébet Rásó, Judit Moldvay
ALK translocation is the 3rd most frequent genetic aberration in lung adenocarcinoma, and several inhibitors are now clinically available in first and second line settings. Accordingly, molecular diagnostics of ALK-positive lung cancer is very important and can be done with the rational combination of several methods. All international recommendations suggest that, except for cytological samples, screening technology for ALK-positive tumors is immunohistochemistry using a validated test. It is highly recommended that in case of ALK protein positive samples gene translocation must be confirmed by fluorescent in situ hybridization (FISH)...
September 20, 2017: Magyar Onkologia
https://www.readbyqxmd.com/read/28931094/ethnicity-and-skin-autofluorescence-based-risk-engines-for-cardiovascular-disease-and-diabetes-mellitus
#2
Muhammad Saeed Ahmad, Torben Kimhofer, Sultan Ahmad, Mohammed Nabil AlAma, Hala Hisham Mosli, Salwa Ibrahim Hindawi, Dennis O Mook-Kanamori, Katarína Šebeková, Zoheir Abdullah Damanhouri, Elaine Holmes
Skin auto fluorescence (SAF) is used as a proxy for the accumulation of advanced glycation end products (AGEs) and has been proposed to stratify patients into cardiovascular disease (CVD) and diabetes mellitus (DM) risk groups. This study evaluates the effects of seven different ethnicities (Arab, Central-East African, Eastern Mediterranean, European, North African, South Asian and Southeast Asian) and gender on SAF as well as validating SAF assessment as a risk estimation tool for CVD and DM in an Arabian cohort...
2017: PloS One
https://www.readbyqxmd.com/read/28931085/hcv-induced-autophagosomes-are-generated-via-homotypic-fusion-of-phagophores-that-mediate-hcv-rna-replication
#3
Linya Wang, Ja Yeon Kim, Helene Minyi Liu, Michael M C Lai, Jing-Hsiung James Ou
Hepatitis C virus (HCV) induces autophagy to promote its replication, including its RNA replication, which can take place on double-membrane vesicles known as autophagosomes. However, how HCV induces the biogenesis of autophagosomes and how HCV RNA replication complex may be assembled on autophagosomes were largely unknown. During autophagy, crescent membrane structures known as phagophores first appear in the cytoplasm, which then progress to become autophagosomes. By conducting electron microscopy and in vitro membrane fusion assay, we found that phagophores induced by HCV underwent homotypic fusion to generate autophagosomes in a process dependent on the SNARE protein syntaxin 7 (STX7)...
September 19, 2017: PLoS Pathogens
https://www.readbyqxmd.com/read/28931067/in-vivo-imaging-system-for-explants-analysis-a-new-approach-for-assessment-of-cell-transplantation-effects-in-large-animal-models
#4
Weronika Zarychta-Wiśniewska, Anna Burdzinska, Radosław Zagozdzon, Bartosz Dybowski, Marta Butrym, Zdzisław Gajewski, Leszek Paczek
INTRODUCTION: Despite spectacular progress in cellular transplantology, there are still many concerns about the fate of transplanted cells. More preclinical studies are needed, especially on large animal models, to bridge the translational gap between basic research and the clinic. Herein, we propose a novel approach in analysis of cell transplantation effects in large animals explants using in vivo imaging system (IVIS®) or similar equipment. MATERIAL AND METHODS: In the in vitro experiment cells labeled with fluorescent membrane dyes: DID (far red) or PKH26 (orange) were visualized with IVIS®...
2017: PloS One
https://www.readbyqxmd.com/read/28931049/the-c5a-c5ar1-axis-controls-the-development-of-experimental-allergic-asthma-independent-of-lysm-expressing-pulmonary-immune-cells
#5
Anna V Wiese, Fanny Ender, Katharina M Quell, Konstantina Antoniou, Tillman Vollbrandt, Peter König, Jörg Köhl, Yves Laumonnier
C5a regulates the development of maladaptive immune responses in allergic asthma mainly through the activation of C5a receptor 1 (C5aR1). Yet, the cell types and the mechanisms underlying this regulation are ill-defined. Recently, we described increased C5aR1 expression in lung tissue eosinophils but decreased expression in airway and pulmonary macrophages as well as in pulmonary CD11b+ conventional dendritic cells (cDCs) and monocyte-derived DCs (moDCs) during the allergic effector phase using a floxed green fluorescent protein (GFP)-C5aR1 knock-in mouse...
2017: PloS One
https://www.readbyqxmd.com/read/28931000/trapping-of-micro-particles-in-nanoplasmonic-optical-lattice
#6
Dinesh Bhalothia, Ya-Tang Yang
The plasmonic optical tweezer has been developed to overcome the diffraction limits of the conventional far field optical tweezer. Plasmonic optical lattice consists of an array of nanostructures, which exhibit a variety of trapping and transport behaviors. We report the experimental procedures to trap micro-particles in a simple square nanoplasmonic optical lattice. We also describe the optical setup and the nanofabrication of a nanoplasmonic array. The optical potential is created by illuminating an array of gold nanodiscs with a Gaussian beam of 980 nm wavelength, and exciting plasmon resonance...
September 5, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28930993/confocal-imaging-of-neuropeptide-y-phluorin-a-technique-to-visualize-insulin-granule-exocytosis-in-intact-murine-and-human-islets
#7
Madina Makhmutova, Tao Liang, Herbert Gaisano, Alejandro Caicedo, Joana Almaça
Insulin secretion plays a central role in glucose homeostasis under normal physiological conditions as well as in disease. Current approaches to study insulin granule exocytosis either use electrophysiology or microscopy coupled to the expression of fluorescent reporters. However most of these techniques have been optimized for clonal cell lines or require dissociating pancreatic islets. In contrast, the method presented here allows for real time visualization of insulin granule exocytosis in intact pancreatic islets...
September 13, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28930782/determining-the-false-negative-rate-using-fluorescence-image-assisted-sentinel-lymph-node-biopsy-in-cutaneous-melanoma
#8
Rafael A Couto, Gregory A Lamaris, Rebecca Knackstedt, Brendan Alleyne, Paul Durand, Steven Rueda, Brian Gastman
INTRODUCTION: Despite the advances in cutaneous melanoma management, the false-negative rates (FNRs) of sentinel lymph node biopsy (SLNB) are still high. These rates are dependent not only on the technique but also on definitional terms and percentage of head/neck melanoma (highest false-negative SLNB). Fluorescence imaging technology is well acquainted in plastic surgery and other specialties. Having demonstrated that fluorescence-assisted SLNB is effective in melanoma, we are interested in determining its FNR...
September 19, 2017: Annals of Plastic Surgery
https://www.readbyqxmd.com/read/28930754/simvastatin-a-novel-stimulator-of-eryptosis-the-suicidal-erythrocyte-death
#9
Abdulla Al Mamun Bhuyan, Sigrid Nüßle, Hang Cao, Shaqiu Zhang, Florian Lang
BACKGROUND/AIMS: The 3-hydroxy-3-methyl-glutaryl-Coenzyme A (HMG-CoA) reductase inhibitor simvastatin has been shown to trigger apoptosis of several cell types. The substance has thus been proposed as an additional treatment of malignancy. Similar to apoptosis of nucleated cells, erythrocytes may enter eryptosis, the suicidal erythrocyte death. Hallmarks of eryptosis include cell shrinkage and cell membrane scrambling with phosphatidylserine translocation to the extracellular face of the erythrocyte cell membrane...
September 20, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28930702/gcamp-expression-in-retinal-ganglion-cells-characterized-using-a-low-cost-fundus-imaging-system
#10
Yao-Chuan Chang, Steven T Walston, Robert H Chow, James D Weiland
OBJECTIVE: Virus-transduced, intracellular-calcium indicators are effective reporters of neural activity, offering the advantage of cell-specific labeling. Due to the existence of an optimal time window for the expression of calcium indicators, a suitable tool for tracking GECI expression in vivo following transduction is highly desirable. APPROACH: We developed a noninvasive imaging approach based on a custom-modified, low-cost fundus viewing system that allowed us to monitor and characterize in vivo bright-field and fluorescence images of the mouse retina...
October 2017: Journal of Neural Engineering
https://www.readbyqxmd.com/read/28930671/cohesin-can-remain-associated-with-chromosomes-during-dna-replication
#11
James D P Rhodes, Judith H I Haarhuis, Jonathan B Grimm, Benjamin D Rowland, Luke D Lavis, Kim A Nasmyth
To ensure disjunction to opposite poles during anaphase, sister chromatids must be held together following DNA replication. This is mediated by cohesin, which is thought to entrap sister DNAs inside a tripartite ring composed of its Smc and kleisin (Scc1) subunits. How such structures are created during S phase is poorly understood, in particular whether they are derived from complexes that had entrapped DNAs prior to replication. To address this, we used selective photobleaching to determine whether cohesin associated with chromatin in G1 persists in situ after replication...
September 19, 2017: Cell Reports
https://www.readbyqxmd.com/read/28930631/direct-evidence-of-intrinsic-blue-fluorescence-from-oligomeric-interfaces-of-human-serum-albumin
#12
Arpan Bhattacharya, Soumitra Bhowmik, Amit K Singh, Prashant Kodgire, Apurba K Das, Tushar Kanti Mukherjee
The molecular origin behind the concentration-dependent intrinsic blue fluorescence of human serum albumin (HSA) is not known yet. This unusual blue fluorescence is believed to be a characteristic feature of amyloid-like fibrils of protein/peptide and originates due to the delocalization of peptide bond electrons through the extended hydrogen bond networks of cross--sheet structure. Herein, by combining the results of spectroscopy, size exclusion chromatography, native gel electrophoresis and confocal microscopy, we have shown that the intrinsic blue fluorescence of HSA exclusively originates from oligomeric interfaces devoid of any amyloid-like fibrillar structure...
September 20, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/28930482/diagnostic-and-prognostic-cytogenetics-of-chronic-myeloid-leukaemia-an-update
#13
Matteo Molica, Fulvio Massaro, Massimo Breccia
Despite the advent of molecular assessment, banding cytogenetics and fluorescence in situ hybridization (FISH) still have a significant role in diagnostic and prognostic approaches to chronic myeloid leukaemia (CML). Area covered. At diagnosis and duringtreatment with tyrosine kinase inhibitors (TKIs), cytogenetics is used to detect the Philadelphia chromosome, with its typical translocation t(9;22)(q34;q11.2), and any additional or other chromosomal aberrations (ACAs and OCAs) that may arise in 5-10% of cases, the latter associated to transformation of the disease in blast phases...
September 20, 2017: Expert Review of Molecular Diagnostics
https://www.readbyqxmd.com/read/28930465/single-molecule-based-white-light-emissive-organic-solids-with-molecular-packing-dependent-thermally-activated-delayed-fluorescence
#14
Yuewei Zhang, Yang Miao, Xiaoxian Song, Yu Gao, Zuolun Zhang, Kaiqi Ye, Yue Wang
White-light emitting single molecules have attracted broad attention because of their great potential for use in flat-panel displays and future light sources. Herein, we report a unique molecule of 3-(diphenylamino)-9H-xanthen-9-one (3-DPH-XO), which was found to exhibit bright white-light emission in the solid state caused by the spontaneous formation of a mixture with different polymorphs. Single-crystal analyses demonstrate that non-covalent interactions (such as π•••π stacking, hydrogen bonding, and C-H•••π interactions) induce different stacking arrangements (polymorphs A, B, and C) with different photophysical properties in a molecular solid...
September 20, 2017: Journal of Physical Chemistry Letters
https://www.readbyqxmd.com/read/28930443/hydrogel-tethering-enhances-interdomain-stabilization-of-single-chain-antibodies
#15
Yijia Xiong, Nicole R Ford, Karen A Hecht, Guritno Roesijadi, Thomas C Squier
Here we identify the importance of molecular crowding agents in the functional stabilization of scFv antibodies. Antibodies were tethered through an engineered calmodulin (CaM) binding peptide into a stimulus-responsive hydrogel composed of poly-(ethylene glycol) (PEG) functionalized CaM. Macromolecular crowding is modulated by transient heating, which acts to decrease effective pore sizes. Using a fluorescent ligand bound to the scFv, frequency-domain fluorescence spectroscopy was used to assess the structural coupling between the VH and VL domains and relationships with functional stabilization...
September 20, 2017: Bioconjugate Chemistry
https://www.readbyqxmd.com/read/28930438/supported-planar-mammalian-membranes-as-models-of-in-vivo-cell-surface-architectures
#16
Han-Yuan Liu, Hannah Grant, Hung-Lun Hsu, Raya Sorkin, Filip Bošković, G J L Wuite, Susan Daniel
Emerging technologies use cell plasma membrane vesicles or 'blebs' as an intermediate to form molecularly complete, planar cell surface mimetics that are compatible with a variety of characterization tools and microscopy methods. This approach enables direct incorporation of membrane proteins into supported lipid bilayers without using detergents and reconstitution and preserves native lipids and membrane species. Such a system can be advantageous as in vitro models of in vivo cell surfaces for study of the roles of membrane proteins as drug targets in drug delivery, host-pathogen interactions, tissue engineering, and many other bioanalytical and sensing applications...
September 20, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28930435/new-triplet-sensitization-routes-for-photon-upconversion-thermally-activated-delayed-fluorescence-molecules-inorganic-nanocrystals-and-singlet-to-triplet-absorption
#17
Nobuhiro Yanai, Nobuo Kimizuka
Photon upconversion based on triplet-triplet annihilation (TTA-UC) has attracted much interest because of its possible applications to renewable energy production and biological fields. In particular, the UC of near-infrared (NIR) light to visible (vis) light is imperative to overcome the Shockley-Queisser limit of single-junction photovoltaic cells, and the efficiency of photocatalytic hydrogen production from water can also be improved with the aid of vis-to-ultraviolet (UV) UC. However, both processes have met limitations in the wavelength range, efficiency, and sensitivity for weak incident light...
September 20, 2017: Accounts of Chemical Research
https://www.readbyqxmd.com/read/28930309/a-global-method-for-handling-fluorescence-spectra-at-high-concentration-derived-from-the-competition-between-emission-and-absorption-of-colloidal-cdte-quantum-dots
#18
Thomas Noblet, Laurent Dreesen, Julie Hottechamps, Christophe Humbert
We investigate the effects of the concentration of CdTe quantum dots (QDs) on their fluorescence in water. The emission spectra, acquired in right angle geometry, exhibit highly variable shapes. The measurements evidence a critical value of the concentration beyond which the intensity and the spectral bandwidth decrease and the fluorescence maximum is redshifted. To account for these observations, we develop a model based on the primary and secondary inner filter effects. The accuracy of the model ensures that the concentration dependent behaviour of QD fluorescence is completely due to inner filter effects...
September 20, 2017: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/28930308/structure-activity-relationship-study-of-proxyphos-chemosensors-for-the-detection-of-proximal-phosphorylation-and-other-phosphate-species
#19
A D Cabral, B I Murcar-Evans, K Toutah, M Bancerz, D Rosa, K Yuen, T B Radu, M Ali, A Penkul, D Kraskouskaya, P T Gunning
Chemosensors for the detection of phosphate-containing biological species are in high need. Detection of proximally phosphorylated sites of PPi and those found in peptides and proteins has been demonstrated using chemosensors containing pyrene, as a fluorescent reporter, and a Zn(2+)-chelate, as a phosphate-binding group. Using these sensors, detection of proximal phosphate groups is afforded by binding of at least two of the sensor molecules to the adjacent phosphates, via the Zn(2+) centres, leading to excimer formation between the pyrene groups and the corresponding shift in emission from 376 to 476 nm...
September 20, 2017: Analyst
https://www.readbyqxmd.com/read/28930228/interaction-of-plant-extracts-with-central-nervous-system-receptors
#20
REVIEW
Kenneth Lundstrom, Huyen Thanh Pham, Long Doan Dinh
Background: Plant extracts have been used in traditional medicine for the treatment of various maladies including neurological diseases. Several central nervous system receptors have been demonstrated to interact with plant extracts and components affecting the pharmacology and thereby potentially playing a role in human disease and treatment. For instance, extracts from Hypericum perforatum (St. John's wort) targeted several CNS receptors. Similarly, extracts from Piper nigrum, Stephania cambodica, and Styphnolobium japonicum exerted inhibition of agonist-induced activity of the human neurokinin-1 receptor...
February 23, 2017: Medicines (Basel, Switzerland)
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