keyword
https://read.qxmd.com/read/38536943/highly-accurate-profiling-of-exosome-phenotypes-using-super-resolution-tricolor-fluorescence-co-localization
#1
JOURNAL ARTICLE
Jinxiu Wei, Kai Zhu, Tingyu Wang, Tongsheng Qi, Zhuyuan Wang, Jia Li, Shenfei Zong, Yiping Cui
Exosomes contain a wealth of proteomic information, presenting promising biomarkers for the noninvasive early diagnosis of diseases, especially cancer. However, it remains a great challenge to accurately and reliably distinguish exosomes secreted from different types of cell lines. Fluorescence immunoassay is frequently used for exosome detection. Nonspecific adsorption in immunoassays is unavoidable and affects the reliability of assay results. Despite the fact that various methods have been proposed to reduce nonspecific adsorption, a more effective method that can eliminate the influence of nonspecific adsorption is still lacking...
March 27, 2024: ACS Nano
https://read.qxmd.com/read/38536618/genome-constitution-and-evolution-of-elymus-atratus-poaceae-triticeae-inferred-from-cytogenetic-and-phylogenetic-analysis
#2
JOURNAL ARTICLE
Lu Tan, Dan-Dan Wu, Chang-Bing Zhang, Yi-Ran Cheng, Li-Na Sha, Xing Fan, Hou-Yang Kang, Yi Wang, Hai-Qin Zhang, Marcial Escudero, Yong-Hong Zhou
BACKGROUND: Elymus atratus (Nevski) Hand.-Mazz. is perennial hexaploid wheatgrass. It was assigned to the genus Elymus L. sensu stricto based on morphological characters. Its genome constitution has not been disentangled yet. OBJECTIVE: To identify the genome constitution and origin of E. atratus. METHODS: In this study, genomic in situ hybridization and fluorescence in situ hybridization, and phylogenetic analysis based on the Acc1, DMC1 and matK sequences were performed...
March 27, 2024: Genes & Genomics
https://read.qxmd.com/read/38536610/continuous-detection-of-cobalt-ions-and-pyrophosphates-by-nac-cdte-quantum-dots-fluorescence-probes
#3
JOURNAL ARTICLE
Wanqiu Chen, Ting Chen, Min Fang, Weiju Zhu, Cun Li
In this work, a simple and sensitive N-acetyl-L-cysteine (NAC)-covered CdTe quantum dots (NAC-CdTe QDs) fluorescence probe for continuous detection of Co2+ and pyrophosphate ions (PPi, P2 O7 4- ) was synthesized. The fluorescence of the quantum dots was significantly quenched by Co2+ through the coordination of Co2+ and the carboxyl groups on the NAC-CdTe quantum dots. Interestingly, the combination of NAC-CdTe quantum dots with Co2+ yields a new fluorescence probe of Co2+ -modified NAC-CdTe QDs (Co2+ @NAC-CdTe)...
March 27, 2024: Journal of Fluorescence
https://read.qxmd.com/read/38536609/synthesis-and-photophysical-properties-of-bipolar-compound-triphenylamine-diazafluorene-carbazole
#4
JOURNAL ARTICLE
Radhakrishnan Sivakumar, Rajamohan Satheesh, Arumugam Manivel, David Contreras, Manidurai Paulraj
Carbazole and triphenylamine, are two well-known hole transporting units that are attached to electron transporting unit 4,5-diazafluorene in a fascinating way to bring out non-planar configuration of a molecule. The synthesized compound exhibits good thermal stability (Td  > 515 °C) and high glass transition temperature (Tg , 191 °C). Optical bandgap (Eg opt ) obtained from solid state absorption spectra was calculated to be 2.93 eV. Solid state photoluminescence spectra displays the emission maxima at 473 nm...
March 27, 2024: Journal of Fluorescence
https://read.qxmd.com/read/38535995/high-internal-phase-emulsion-for-constructing-chiral-helical-polymer-based-circularly-polarized-luminescent-porous-materials
#5
JOURNAL ARTICLE
Yanan Wang, Hai Zhong, Biao Zhao, Jianping Deng
Polymerized high internal phase emulsions (polyHIPEs) with circularly polarized luminescence (CPL), as an interesting class of porous materials, are of great significance for the development of CPL porous materials but have not been reported so far. Herein, we report the construction of polyHIPE-based CPL porous materials, taking advantage of an adsorption strategy. The pristine polyHIPEs constructed by chiral helical polymers, which acted as a chiral microenvironment, were fabricated by coordination polymerization of chiral acetylene monomers ( R / S -SA) using HIPEs as templates...
March 27, 2024: ACS Applied Materials & Interfaces
https://read.qxmd.com/read/38535895/a-multi-colour-fluorogenic-tag-and-its-application-in-candida-albicans
#6
JOURNAL ARTICLE
Jonas Devos, Patrick Van Dijck, Wouter Van Genechten
Fluorescent proteins (FPs) have always been a crucial part of molecular research in life sciences, including the research into the human fungal pathogen Candida albicans, but have obvious shortcomings such as their relatively large size and long maturation time. However, the next generation of FPs overcome these issues and rely on the binding of a fluorogen for the protein to become fluorescently active. This generation of FPs includes the improved version of Fluorescence activating and Absorption Shifting Tag (iFAST)...
March 2024: Microbiology
https://read.qxmd.com/read/38535872/detection-and-phylogenetic-analysis-of-caprine-arthritis-encephalitis-virus-using-taqman-based-qpcr-in-eastern-china
#7
JOURNAL ARTICLE
Yutong Tian, Hailong Zhang, Yan Zhang, Xinya Zhang, Zhilei Guan, Junjie Zhang, Yafeng Qiu, Beibei Li, Ke Liu, Zongjie Li, Donghua Shao, Peng Li, Zhiyong Ma, Jianchao Wei
Caprine arthritis encephalitis is an infectious disease caused by the caprine arthritis encephalitis virus that infects goats, sheep, and other small ruminants. An outbreak of CAEV could be extremely harmful to the goat farming industry and could cause severe economic losses. We designed specific primers and probes for the gag gene and established a TaqMan real-time quantitative polymerase chain reaction assay. This method's correlation coefficient (R2 ) was >0.999, and the sensitivity of the assay to the plasmid-carried partial gag gene was approximately 10 copies/µL, 1000 times higher than that of conventional PCR...
March 21, 2024: Veterinary Sciences
https://read.qxmd.com/read/38535840/in-vitro-antimycotic-activity-and-structural-damage-against-canine-malassezia-pachydermatis-strains-caused-by-mexican-stingless-bee-propolis
#8
JOURNAL ARTICLE
Diana Berenice Fuentes Esquivel, Betsabé Rodríguez Pérez, Nelly Tovar Betancourt, Carlos Gerardo García Tovar, José Guillermo Penieres Carrillo, Florentina Hernández Galindo, Javier Pérez Flores, Tonatiuh Alejandro Cruz Sánchez
This work describes the antimycotic activity of propolis from the stingless bees Scaptotrigona mexicana and Tetragonisca angustula , collected from two Mexican regions (Veracruz and Chiapas, respectively), against three clinical isolates and the reference strain ATCC 14522 of Malassezia pachydermatis , the causative agent of canine otitis. The chemical components of the ethanolic extracts of propolis were determined by gas chromatography coupled with mass spectrometry (GC-MS), and sesquiterpenes were the predominant compounds...
February 28, 2024: Veterinary Sciences
https://read.qxmd.com/read/38535838/identification-of-sarcocystis-spp-in-slaughtered-sheep-from-spain-and-evaluation-of-bradyzoite-viability-after-freezing
#9
JOURNAL ARTICLE
María Paz Peris, María Jesús Gracia, Bernardino Moreno, Paula Juan-Puente, Mariano Morales, María Serrano, María Dolores Manzano, Nabil Halaihel, Juan Badiola, Juan Antonio Castillo
Sarcocystis spp. are complex apicomplexan parasites that cause a substantial economic impact on livestock used for meat production. These parasites are present worldwide. Our study aimed to identify Sarcocystis species affecting sheep meat in southern-central Spain and to evaluate the effectiveness of freezing for parasite inactivation. A total of 210 condemned samples of sheep meat were thoroughly assessed grossly and microscopically; the presence of macro- and microcysts was confirmed. The samples were then frozen at -20 °C for various time intervals (24, 48, 72, 96, 120, and 144 h) and compared with untreated samples...
February 27, 2024: Veterinary Sciences
https://read.qxmd.com/read/38535822/cationicity-enhancement-on-the-hydrophilic-face-of-ctriporin-significantly-reduces-its-hemolytic-activity-and-improves-the-antimicrobial-activity-against-antibiotic-resistant-eskape-pathogens
#10
JOURNAL ARTICLE
Xudong Luo, Huan Deng, Li Ding, Xiangdong Ye, Fang Sun, Chenhu Qin, Zongyun Chen
The ESKAPE pathogen-associated antimicrobial resistance is a global public health issue, and novel therapeutic strategies are urgently needed. The short cationic antimicrobial peptide (AMP) family represents an important subfamily of scorpion-derived AMPs, but high hemolysis and poor antimicrobial activity hinder their therapeutic application. Here, we recomposed the hydrophilic face of Ctriporin through lysine substitution. We observed non-linear correlations between the physiochemical properties of the peptides and their activities, and significant deviations regarding the changes of antimicrobial activities against different bacterial species, as well as hemolytic activity...
March 18, 2024: Toxins
https://read.qxmd.com/read/38535785/magnetic-molecularly-imprinted-polymers-for-selective-extraction-of-aflatoxins-from-feeds
#11
JOURNAL ARTICLE
María Del Carmen Pérez-Álvarez, Natalia Arroyo-Manzanares, Natalia Campillo, Pilar Viñas
Magnetic molecularly imprinted polymers (MMIPs) have fused molecular imprinting technology with magnetic separation technology, emerging as an innovative material capable of recognizing specific molecules and efficiently separating target substances. Their application to the extraction and purification of mycotoxins has great potential, due to the toxicity and economic impact of these contaminants. In this work, MMIP has been proposed as a sample treatment for the determination of main four aflatoxins (B1 , B2 , G1 and G2 ) in pig feed...
February 29, 2024: Toxins
https://read.qxmd.com/read/38535700/biosynthesis-of-cu-in-s-nanoparticles-by-a-yeast-isolated-from-union-glacier-antarctica-a-platform-for-enhanced-quantum-dot-sensitized-solar-cells
#12
JOURNAL ARTICLE
Carolina Arriaza-Echanes, Jessica L Campo-Giraldo, Felipe Valenzuela-Ibaceta, Javiera Ramos-Zúñiga, José M Pérez-Donoso
In recent years, the utilization of extremophile microorganisms for the synthesis of metal nanoparticles, featuring enhanced properties and diverse compositions, has emerged as a sustainable strategy to generate high-quality nanomaterials with unique characteristics. Our study focuses on the biosynthesis of Cu-In-S (CIS) nanoparticles, which has garnered considerable attention in the past decade due to their low toxicity and versatile applications in biomedicine and solar cells. Despite this interest, there is a notable absence of reports on biological methods for CIS nanoparticle synthesis...
March 21, 2024: Nanomaterials
https://read.qxmd.com/read/38535593/characterization-of-a-novel-species-of-legionella-isolated-from-a-healthcare-facility-legionella-resiliens-sp-nov
#13
JOURNAL ARTICLE
Sandra Cristino, Maria Rosaria Pascale, Federica Marino, Carlo Derelitto, Silvano Salaris, Massimiliano Orsini, Stefano Squarzoni, Antonella Grottola, Luna Girolamini
Two Legionella -like isolates, 8cVS16T and 9fVS26, were isolated from a water distribution system (WDS) in a healthcare facility. Cells were Gram- and Ziehl Neelsen-stain-negative, rod-shaped, motile, and exhibited a blue-white fluorescence under Wood's lamp at 365 nm. The strains grew in a range of 32-37 °C on BCYE with L-cysteine (C ys +), GVPC, and MWY agar medium, with a positive reaction for oxidase, catalase, and gelatinase. The dominant fatty acids were summed features 3 (C16:1 ω7c/C16:1 ω6c) (27...
March 14, 2024: Pathogens
https://read.qxmd.com/read/38535251/gold-nanoparticle-based-colorimetric-and-fluorescent-dual-mode-lateral-flow-immunoassay-for-sars-cov-2-detection
#14
JOURNAL ARTICLE
Ying Gan, Hefan Zhang, Jing Liu, Fuqin He, Fengheng Li, Ao Li, Man Xing, Dongming Zhou, Shan-Yu Fung, Hong Yang
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection caused the COVID-19 pandemic, impacting the global economy and medical system due to its fast spread and extremely high infectivity. Efficient control of the spread of the disease relies on a fast, accurate, and convenient detection system for the early screening of the infected population. Although reverse transcription-quantitative polymerase chain reaction (RT-qPCR) is the gold-standard method for SARS-CoV-2 RNA analysis, it has complex experimental procedures and relies on expensive instruments and professional operators...
February 27, 2024: Journal of Functional Biomaterials
https://read.qxmd.com/read/38535217/died-or-not-dyed-assessment-of-viability-and-vitality-dyes-on-planktonic-cells-and-biofilms-from-candida-parapsilosis
#15
JOURNAL ARTICLE
Betsy Verónica Arévalo-Jaimes, Eduard Torrents
Viability and vitality assays play a crucial role in assessing the effectiveness of novel therapeutic approaches, with stain-based methods providing speed and objectivity. However, their application in yeast research lacks consensus. This study aimed to assess the performance of four common dyes on C. parapsilosis planktonic cells as well as sessile cells that form well-structured biofilms (treated and not treated with amphotericin B). Viability assessment employed Syto-9 (S9), thiazole orange (TO), and propidium iodide (PI)...
March 11, 2024: Journal of Fungi (Basel, Switzerland)
https://read.qxmd.com/read/38535206/comparison-of-crispr-mad7-and-crispr-cas9-for-gene-disruptions-in-komagataella-phaffii
#16
JOURNAL ARTICLE
Kirill Smirnov, Florian Weiss, Anna-Maria Hatzl, Lukas Rieder, Kjeld Olesen, Sanne Jensen, Anton Glieder
CRISPR (clustered regularly interspaced short palindromic repeats)-based technologies are powerful, programmable tools for site-directed genome modifications. After successful adaptation and efficient use of CRISPR-Cas9 for genome engineering in methylotrophic yeast Komagataella phaffii , a broader variety of employable endonucleases was desired to increase the experimental flexibility and to provide alternatives in case there are specific legal restrictions in industrial research due to the intellectual property rights (IPRs) of third parties...
March 5, 2024: Journal of Fungi (Basel, Switzerland)
https://read.qxmd.com/read/38534858/size-control-of-biomimetic-curved-edge-vaterite-with-chiral-toroid-morphology-via-sonochemical-synthesis
#17
JOURNAL ARTICLE
Ki Ha Min, Dong Hyun Kim, Seung Pil Pack
The metastable vaterite polymorph of calcium carbonate (CaCO3 ) holds significant practical importance, particularly in regenerative medicine, drug delivery, and various personal care products. Controlling the size and morphology of vaterite particles is crucial for biomedical applications. This study explored the synergistic effect of ultrasonic (US) irradiation and acidic amino acids on CaCO3 synthesis, specifically the size, dispersity, and crystallographic phase of curved-edge vaterite with chiral toroids (chiral-curved vaterite)...
March 13, 2024: Biomimetics
https://read.qxmd.com/read/38534785/cellular-organelle-related-transcriptomic-profile-abnormalities-in-neuronopathic-types-of-mucopolysaccharidosis-a-comparison-with-other-neurodegenerative-diseases
#18
JOURNAL ARTICLE
Karolina Wiśniewska, Lidia Gaffke, Magdalena Żabińska, Grzegorz Węgrzyn, Karolina Pierzynowska
Mucopolysaccharidoses (MPS) are a group of diseases caused by mutations in genes encoding lysosomal enzymes that catalyze reactions of glycosaminoglycan (GAG) degradation. As a result, GAGs accumulate in lysosomes, impairing the proper functioning of entire cells and tissues. There are 14 types/subtypes of MPS, which are differentiated by the kind(s) of accumulated GAG(s) and the type of a non-functional lysosomal enzyme. Some of these types (severe forms of MPS types I and II, MPS III, and MPS VII) are characterized by extensive central nervous system disorders...
March 21, 2024: Current Issues in Molecular Biology
https://read.qxmd.com/read/38534604/a-fully-integrated-microfluidic-device-with-immobilized-dyes-for-simultaneous-detection-of-cell-free-dna-and-histones-from-plasma-using-dehydrated-agarose-gates
#19
JOURNAL ARTICLE
Shadi Shahriari, P Ravi Selvaganapathy
Sepsis, a life-threatening condition resulting from a failing host response to infection, causes millions of deaths annually, necessitating rapid and simple prognostic assessments. A variety of genomic and proteomic biomarkers have been developed for sepsis. For example, it has been shown that the level of plasma cell-free DNA (cfDNA) and circulating histones increases considerably during sepsis, and they are linked with sepsis severity and mortality. Developing a diagnostic tool that is capable of assessing such diverse biomarkers is challenging as the detection methodology is quite different for each...
March 8, 2024: Gels
https://read.qxmd.com/read/38534575/disulfide-cross-linked-polymeric-redox-responsive-nanocarrier-based-on-heparin-chitosan-and-lipoic-acid-improved-drug-accumulation-increased-cytotoxicity-and-selectivity-to-leukemia-cells-by-tumor-targeting-via-aikido-principle
#20
JOURNAL ARTICLE
Igor D Zlotnikov, Alexander A Ezhov, Natalia V Dobryakova, Elena V Kudryashova
We have developed a micellar formulation of anticancer drugs based on chitosan and heparin grafted with lipoic and oleic acids that can release the cytotoxic cargo (doxorubicin) in response to external stimuli, such as increased glutathione concentration-a hallmark of cancer. Natural polysaccharides (heparin and chitosan) provide the pH sensitivity of the nanocarrier: the release of doxorubicin (Dox) is enhanced in a slightly acidic environment (tumor microenvironment). Fatty acid residues are necessary for the formation of nanoparticles (micelles) and solubilization of cytostatics in a hydrophobic core...
February 20, 2024: Gels
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