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https://www.readbyqxmd.com/read/28821156/physicochemical-interactions-of-cycloamylose-with-phenolic-compounds
#1
Shin-Joung Rho, Saehun Mun, Jung Sun Hong, Young-Lim Kim, Ha V Do, Young-Wan Kim, Sang-Ik Han, Yong-Ro Kim
The complex formation capability of cycloamylose (CA), having a degree of polymerization of 23-45, with phenolic compounds (PCs) was investigated using various physicochemical techniques. The fluorescence intensity of PCs increased and then reached a plateau at 10-20mM cyclodextrin, while it continued to increase at up to 60mM CA. Thermodynamic data of CA complexes with PCs revealed that the binding process was primarily enthalpy-driven and spontaneous. CA favored to form the most stable complex with chlorogenic acid (CHA) among all PCs...
October 15, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28821130/reaction-pattern-differences-impact-physical-properties-of-starches-derivatized-to-the-same-extent-in-a-model-cross-linking-system
#2
Jung Sun Hong, Sara V Gomand, Kerry C Huber, Jan A Delcour
This study investigated the physical properties of maize (MS) and wheat (WS) starches derivatized with 5-(4,6-dichlorotriazinyl)aminofluorescein (model cross-linking system) to have the same overall fluorescence intensity on starch molecules, but reacted either more uniformly throughout granules (UD) or more at granule surfaces (SD). Both MS and WS derivatives had lower swelling powers (SP) at 90°C than their respective native starches. The UD derivatives had lower SP (90°C) and greater retrogradation enthalpies than did SD derivatives, consistent with their lower peak and higher setback pasting viscosities...
October 15, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28821116/active-release-coating-of-multilayer-assembled-branched-and-ionic-%C3%AE-cyclodextrins-onto-poly-ethylene-terephthalate
#3
Leyre Pérez-Álvarez, Leire Ruiz-Rubio, Erlantz Lizundia, Estíbaliz Hernáez, Luis M León, Jose Luis Vilas-Vilela
Branched ionic cyclodextrins multilayers were assembled onto the surface of previously aminolyzed poly(ethylene terephthalate) in order to construct local drug delivery tailored platforms with potential applicability as materials for indwelling medical devices. The construction of the multilayers took place by the alternate deposition of ionic cyclodextrin derivatives and was characterized by surface zeta-potential measurements, X-ray photoelectron spectroscopy and, after fluorescent labeling of anionic branched cyclodextrins, by UV-vis spectroscopy and confocal fluorescence spectroscopy...
October 15, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28821106/quantitative-assay-of-%C3%AE-1-3-%C3%AE-1-6-glucans-from-fermentation-broth-using-aniline-blue
#4
Steven Koenig, Broder Rühmann, Volker Sieber, Jochen Schmid
An aniline blue fluorescence assay was constructed to quantify two fungal β-(1,3)-glucans with β-(1,6)-sidechains: scleroglucan and schizophyllan. Calibration curves were linear in the range from 30mg/l to 6g/l with R(2)>99.8%. The assay was robust against d-glucose (50 g/l), oxalic acid (22.5g/l), protein (0.667g/l), and KCl (13.3g/l). Sclerotium rolfsii and Schizophyllum commune cultures were sampled, and the test results of the assay and alcohol precipitation were compared. The correlation between assay results of samples at different stages of the purification with the values from the precipitation of the polymers was the best in the supernatant for S...
October 15, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28821076/confirmation-and-measurement-of-hydrophobic-interaction-in-sol-gel-system-of-konjac-glucomannan-with-different-degree-of-deacetylation
#5
Chen Xin, Jian Chen, Hongshan Liang, Jiawei Wan, Jing Li, Bin Li
Fluorescence measurement was used as a tool to study the hydrophobic interaction in sol-gel system of konjac glucomannan (KGM) with different degree of deacetylation (DD). In the concentration from 0 to 10g/L, I1/I3 ratio of native KGM declined from 1.75 to 1.25. Before and after heating, the critical associating concentration of the native KGM was 0.60g/L and 0.95g/L respectively, but this value of deacetylated KGM (DD=32.58%) was 0.58g/L and 0.45g/L respectively. The addition of Na2SO4 and NaSCN exhibited more significant influence on deacetylated KGM compared with native KGM...
October 15, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28821051/a-novel-self-assembly-lentinan-tetraphenylethylene%C3%A2-composite-with-strong-blue%C3%A2-fluorescence-in-water-and-its-properties
#6
Mengxia Li, Pan Chen, Min Xu, Xiaojuan Xu
We report a unique self-assembly of lentinan, a triple helical β-(1→3)-glucan (t-LNT), in water. By molecular dynamics simulation, it was found that t-LNT aggregated preferentially along the chain direction to form long chains, accompanied by side-direction linkage to form branches. Transmission electron microscopy images demonstrated that t-LNT formed dendrite-like fibers, which further formed fishnet-like porous/mesoporous aggregates with increasing concentration. The meshes in the fishnet were ascribed to the intersection of branches...
October 15, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28820991/robust-evaluation-of-performance-monitoring-options-for-ozone-disinfection-in-water-recycling-using-bayesian-analysis
#7
Guido Carvajal, Amos Branch, Philipp Michel, Scott A Sisson, David J Roser, Jörg E Drewes, Stuart J Khan
Ozonation of wastewater has gained popularity because of its effectiveness in removing colour, UV absorbance, trace organic chemicals, and pathogens. Due to the rapid reaction of ozone with organic compounds, dissolved ozone is often not measurable and therefore, the common disinfection controlling parameter, concentration integrated over contact time (CT) cannot be obtained. In such cases, alternative parameters have been shown to be useful as surrogate measures for microbial removal including change in UV254 absorbance (ΔUVA), change in total fluorescence (ΔTF), or O3:TOC (or O3:DOC)...
August 1, 2017: Water Research
https://www.readbyqxmd.com/read/28820976/a-hydrophobic-organelle-probe-based-on-aggregation-induced-emission-nanosuspension-preparation-and-direct-use-for-endoplasmic-reticulum-imaging-in-living-cells
#8
Sichao Zheng, Cuihong Huang, Xuyan Zhao, Yong Zhang, Shuwen Liu, Qiuhua Zhu
Organic fluorophores have a wide range of biological uses and are usually needed to be prepared as water-soluble compounds or nanoparticles for applications in aqueous biosystems owing to their hydrophobic properties, which often is a complex, time-consuming and high-cost process. Here, the nanoparticle preparation of hydrophobic fluorophores and their application in cell imaging have been investigated. It was found: a) fetal bovine serum (FBS) shows an excellent dispersion effect on hydrophobic small-molecule organic compounds; b) a hydrophobic C6-unsubstituted tetrahydropyrimidine (Me-THP-Naph) can be prepared as nanosuspensions utilizing cell culture medium with 10% FBS and directly be used as a specific real-time imaging probe for the endoplasmic reticulum (ER), a dynamic organelle playing a crucial role in many cellular processes...
August 8, 2017: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/28820972/fluorescent-pseudorotaxanes-of-a-quinodicarbocyanine-dye-with-gamma-cyclodextrin
#9
Olivia M Bernstein, Tiffany E McGee, Lisa E Silzel, John W Silzel
Spectrophotometric titration of buffered solutions of gamma cyclodextrin (γCD) and 1,1'-diethyl,2,2'-dicarbocyanine (DDI) demonstrates extension of the known 1:2 host:guest complex to form a previously unreported 2:2 complex near the γCD solubility limit. Though DDI is predominantly hosted as a non-fluorescent H-aggregate, both complexes exist in respective equilibria with two secondary complexes hosting unaggregated DDI as 1:1 and 2:1 complexes. The 2:1 complex exhibits significant fluorescence emission, with a quantum yield six times that of DDI in organic solvents, but ten times lower than that of an analogous indodicarbocyanine...
August 3, 2017: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/28820971/ultrasensitive-detection-of-ag-i-based-on-the-conformational-switching-of-a-multifunctional-aptamer-probe-induced-by-silver-i
#10
Yu-Feng Zhu, Yong-Sheng Wang, Bin Zhou, Yan-Qin Huang, Xue-Jiao Li, Si-Han Chen, Xiao-Feng Wang, Xian Tang
We for the first time confirmed that the low concentrations of Ag(I) could induce a silver specific aptamer probe (SAP) from a random coil sequence form to G-quadruplex structure. Thereby, a novel highly sensitive fluorescence strategy for silver(I) assay was established. The designed multifunctional SAP could act as a recognition element for Ag(I) and a signal reporter. The use of such a SAP can ultrasensitively and selectively detect Ag(I), giving a detection limit down to 0.64nM. This is much lower than those reported by related literatures...
August 10, 2017: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/28820961/optimized-streptavidin-for-fluorescent-labeling-of-biotinylated-targets
#11
Vesa P Hytönen
In order to develop a streptavidin-biotin system with optimal performance in fluorescent labeling, in this issue of Cell Chemical Biology, Jacobsen et al. (2017) focused on changing the surface density of amino groups present on streptavidin via lysine mutagenesis. The streptavidin mutant containing only one free amino group was found superior to other streptavidin variants and was named Flavidin for fluorophore-friendly streptavidin.
August 17, 2017: Cell Chemical Biology
https://www.readbyqxmd.com/read/28820953/a-fluorescence-based-high-throughput-assay-for-the-identification-of-anticancer-reagents-targeting-fructose-1-6-bisphosphate-aldolase
#12
Eun Jeong Cho, Ashwini K Devkota, Gabriel Stancu, Ramakrishna Edupunganti, Garth Powis, Kevin N Dalby
A high rate of glycolysis, which supplies energy and materials for anabolism, is observed in a wide range of tumor cells, making it a potential pathway to control cancer growth. ALDOA is a multifunctional enzyme in the glycolytic pathway and also promotes HIF-1α, which is of importance in hypoxic solid tumors. The current method for assaying ALDOA activity involves monitoring the consumption of NADH in vitro using absorbance or intrinsic fluorescence via a coupled enzymatic reaction. Here, we report the development of a homogeneous biochemical assay that can overcome limitations of current methods, in particular for the application of high-throughput drug screening...
August 1, 2017: SLAS Discovery
https://www.readbyqxmd.com/read/28820937/impact-of-shell-thickness-on-photoluminescence-and-optical-activity-in-chiral-cdse-cds-core-shell-quantum-dots
#13
Finn Purcell-Milton, Anastasia K Visheratina, Vera A Kuznetsova, Aisling Ryan, Anna O Orlova, Yurii K Gun'ko
Core/Shell quantum dots (QDs) are of high scientific and technological importance as these nanomaterials have found a number of valuable applications. In this paper, we have investigated the dependence of optical activity and photoluminescence upon CdS shell thickness in a range of core-shell structured CdSe/CdS QDs capped with chiral ligands. For our study, five samples of CdSe/CdS were synthesised utilizing successive ion layer adsorption and reaction (SILAR) to vary the thickness of the CdS shell from 0...
August 18, 2017: ACS Nano
https://www.readbyqxmd.com/read/28820934/supramolecular-sensors-for-opiates-and-their-metabolites
#14
Elena G Shcherbakova, Ben Zhang, Samer Gozem, Tsuyoshi Minami, Peter Y Zavalij, Mariia Pushina, Lyle Isaacs, Pavel Anzenbacher
The present work develops a sensing approach for opiates using acyclic cucurbituril (aCBs) sensors comprising four glycouril units terminated on both ends with naphthalene fluorophore walls. The connectivity between the glycourils and the naphthalene rings largely defines the opening size of the cucurbituril cavity and its diameter. The large hydrophobic binding cavity is flexible and is able to adapt to guests of various size and topology. The recognition event between the aCBs and guests results in modification of the fluorescence of the terminal walls, a fluorescence response that can be used to sense the drugs of abuse morphine, heroin and oxycodone as well as their metabolites...
August 18, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/28820625/multimodal-image-analysis-of-the-retina-in-hunter-syndrome-mucopolysaccharidosis-type-ii-case-report
#15
Isadora Darriba Macedo Salvucci, Simone Finzi, Maria Kiyoko Oyamada, Chong Ae Kim, Sérgio Luis Gianotti Pimentel
INTRODUCTION: We report a case of retinal and posterior ocular findings in a 33-year-old man diagnosed with Hunter syndrome (Mucopolysaccharidosis type II) in a multimodal imaging way. CASE PRESENTATION: Our patient was complaining of blurred night vision for the past 3 years. He had not received any systemic treatment for Hunter syndrome. Vision acuity was 20/20 in both eyes and corneas were clear. Fundus examination revealed bilateral crowded and hyperemic optic nerve heads (elevated in the ocular ultrasound) and areas of subretinal hypopigmentation...
August 18, 2017: Ophthalmic Genetics
https://www.readbyqxmd.com/read/28820595/vibronic-origin-of-the-qy-absorption-tail-of-bacteriochlorophyll-a-verified-by-fluorescence-excitation-spectroscopy-and-quantum-chemical-simulations
#16
Kristjan Leiger, Juha Matti Linnanto, Arvi Freiberg
The long-wavelength tail of the lowest-energy Qy singlet absorption band of bacteriochlorophyll a in triethylamine peaking at 768.6 nm was examined by means of fluorescence excitation spectroscopy at ambient temperature of 22±1°C. The tail, usually considered a Gaussian, is in fact weakening quasi-exponentially, being clearly evident as far as 940 nm, nearly 2400 cm(-1) (~12 kBT) away from the absorption peak. Quantum chemical simulations identified vibronic transitions from the thermally populated normal modes and their overtones in the ground electronic state as the origin of this tail...
August 18, 2017: Journal of Physical Chemistry Letters
https://www.readbyqxmd.com/read/28820590/conformational-heterogeneity-in-a-fully-complementary-dna-three-way-junction-with-a-gc-rich-branchpoint
#17
Anita Toulmin, Laura E Baltierra-Jasso, Michael J Morten, Tara Sabir, Peter McGlynn, Gunnar F Schröder, Brian O Smith, Steven W Magennis
DNA three-way junctions (3WJs) are branched structures that serve as important biological intermediates and as components in DNA nanostructures. We recently derived the global structure of a fully-complementary 3WJ and found that it contained unpaired bases at the branchpoint, in support of previous observations of branch flexibility and branchpoint reactivity. By combining high-resolution single-molecule FRET, molecular modeling, time-resolved ensemble fluorescence spectroscopy and the first 19F NMR observations of fully complementary 3WJs, we now show that the 3WJ structure can adopt multiple distinct conformations depending upon the sequence at the branchpoint...
August 18, 2017: Biochemistry
https://www.readbyqxmd.com/read/28820580/enhanced-fluorescence-emission-and-singlet-oxygen-generation-of-photosensitizers-embedded-in-injectable-hydrogels-for-imaging-guided-photodynamic-cancer-therapy
#18
Liu-Yuan Xia, Xiaodong Zhang, Meng Cao, Zhan Chen, Fu-Gen Wu
Benefiting from their inherent localized and controlled release properties, hydrogels are ideal delivery systems for therapeutic drugs or nanoparticles. In particular, the applications of hydrogels for the delivery and release of photo-responsive drugs or nanoparticles are receiving increasing attention. However, the effect of the hydrogel matrix on the fluorescence emission and singlet oxygen generation efficiency of the embedded photosensitizers (PSs) has not been clarified. Herein, meso-tetrakis (1-methylpyridinium-4-yl) porphyrin (TMPyP) as a water-soluble PS is encapsulated into an injectable hydrogel formed by glycol chitosan and dibenzaldehyde-terminated telechelic poly(ethylene glycol)...
August 18, 2017: Biomacromolecules
https://www.readbyqxmd.com/read/28820355/alpha-particles-and-x-rays-interact-in-inducing-dna-damage-in-u2os-cells
#19
Alice Sollazzo, Beata Brzozowska, Lei Cheng, Lovisa Lundholm, Siamak Haghdoost, Harry Scherthan, Andrzej Wojcik
Survivors of the atomic bombings of Hiroshima and Nagasaki are monitored for health effects within the Life Span Study (LSS). The LSS results represent the most important source of data about cancer effects from ionizing radiation exposure, which forms the foundation for the radiation protection system. One uncertainty connected to deriving universal risk factors from these results is related to the problem of mixed radiation qualities. The A-bomb explosions generated a mixed beam of the sparsely ionizing gamma radiation and densely ionizing neutrons...
August 18, 2017: Radiation Research
https://www.readbyqxmd.com/read/28820347/association-between-eye-diagnosis-and-positive-syphilis-test-results-in-a-large-urban-sexually-transmitted-infection-primary-care-clinic-population
#20
Ann-Marie Lobo, Yan Gao, Laura Rusie, Magda Houlberg, Supriya D Mehta
In 2015, the Centers for Disease Control and Prevention (CDC) and the American Academy of Ophthalmology (AAO) released clinical advisories on rising cases of ocular syphilis. We examined the association between eye disease and syphilis infection among primary care and sexually transmitted infection (STI) clinic patients attending an urban lesbian, gay, bisexual, transgender (LGBT) health center. We conducted a retrospective medical record review of all patients who underwent syphilis testing at Howard Brown Health between 1 January 2010 and 31 December 2015...
January 1, 2017: International Journal of STD & AIDS
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