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https://www.readbyqxmd.com/read/27926939/a-bystander-mechanism-explains-the-specific-phenotype-of-a-broadly-expressed-misfolded-protein
#1
Lauren Klabonski, Ji Zha, Lakshana Senthilkumar, Tali Gidalevitz
Misfolded proteins in transgenic models of conformational diseases interfere with proteostasis machinery and compromise the function of many structurally and functionally unrelated metastable proteins. This collateral damage to cellular proteins has been termed 'bystander' mechanism. How a single misfolded protein overwhelms the proteostasis, and how broadly-expressed mutant proteins cause cell type-selective phenotypes in disease are open questions. We tested the gain-of-function mechanism of a R37C folding mutation in an endogenous IGF-like C...
December 2016: PLoS Genetics
https://www.readbyqxmd.com/read/27926850/background-suppression-in-imaging-gold-nanorods-through-detection-of-anti-stokes-emission
#2
Aquiles Carattino, Veer I P Keizer, Marcel J M Schaaf, Michel Orrit
Metallic nanoparticles have opened the possibility of imaging, tracking, and manipulating biological samples without time limitations. Their low photoluminescence quantum yield, however, makes them hard to detect under high background conditions. In this study we show that it is possible to image gold nanorods by detecting their anti-Stokes emission under resonant excitation. We show that even in the membrane of a cell containing the fluorescent dye Atto 647N, the signal/background of the anti-Stokes emission can be >10, while it is impossible to image the particles with the Stokes emission...
December 6, 2016: Biophysical Journal
https://www.readbyqxmd.com/read/27926845/membrane-bound-alpha-synuclein-clusters-induce-impaired-lipid-diffusion-and-increased-lipid-packing
#3
Aditya Iyer, Nathalie Schilderink, Mireille M A E Claessens, Vinod Subramaniam
The aggregation of membrane-bound α-synuclein (αS) into oligomers and/or amyloid fibrils has been suggested to cause membrane damage in in vitro model phospholipid membrane systems and in vivo. In this study, we investigate how αS interactions that precede the formation of well-defined aggregates influence physical membrane properties. Using three truncated variants of αS with different aggregation propensities and comparable phospholipid membrane binding affinities we show, using fluorescence recovery after photobleaching (FRAP) and fluorescence anisotropy measurements, that formation of αS clusters on supported lipid bilayers (SLBs) impairs lateral lipid diffusion and increases lipid packing beneath the αS clusters...
December 6, 2016: Biophysical Journal
https://www.readbyqxmd.com/read/27926778/met-tyrosine-kinase-receptor-expression-and-amplification-as-prognostic-biomarkers-of-survival-in-gastroesophageal-adenocarcinoma
#4
Daniel V T Catenacci, Agnes Ang, Wei-Li Liao, Jing Shen, Emily O'Day, Robert D Loberg, Fabiola Cecchi, Todd Hembrough, Annamaria Ruzzo, Francesco Graziano
BACKGROUND: MET gene amplification and Met protein overexpression may be associated with a poor prognosis. The MET/Met status is typically determined with fluorescence in situ hybridization (FISH) and immunohistochemistry (IHC), respectively. Targeted proteomics uses mass spectrometry-based selected reaction monitoring (SRM) to accurately quantitate Met expression. FISH, IHC, and SRM analyses were compared to characterize the prognostic value of MET/Met in gastroesophageal adenocarcinoma (GEC)...
December 7, 2016: Cancer
https://www.readbyqxmd.com/read/27925692/a-method-to-generate-and-analyze-modified-myristoylated-proteins
#5
Huanyao Gao, Wei Sun, Zhiquan Song, Yanbao Yu, Li Wang, Xian Chen, Qisheng Zhang
Covalent lipid modification of proteins is essential to their cellular localizations and functions. Engineered lipid motif coupled with bio-orthogonal chemistry has been utilized to identify myristoylated or palmitoylated proteins in cells. However, whether modified proteins have similar properties as endogenous ones has not been well investigated mainly due to lack of methods to generate and analyze purified proteins. We have developed a method that utilizes metabolic interference and mass spectrometry to produce and analyze modified, myristoylated small GTPase ADP-ribosylation factor 1 (Arf1)...
December 7, 2016: Chembiochem: a European Journal of Chemical Biology
https://www.readbyqxmd.com/read/27925685/in-vitro-characterization-of-synthoplate-%C3%A2-synthetic-platelet-technology-and-its-in-vivo-evaluation-in-severely-thrombocytopenic-mice
#6
Meenal Shukla, Ujjal D S Sekhon, Venkaiah Betapudi, Wei Li, DaShawn A Hickman, Christa L Pawlowski, Mitchell R Dyer, Matthew D Neal, Keith R McCrae, Anirban Sen Gupta
BACKGROUND: Platelet transfusion applications face severe challenges due to the limited availability and portability, high risk of contamination and short shelf-life of platelets. Therefore there is significant interest in synthetic platelet substitutes that can render hemostasis while avoiding these issues. Platelets promote hemostasis by injury site-selective adhesion and aggregation, and propagation of coagulation reactions on their membrane. Based on these mechanisms, we have developed a synthetic platelet technology (SynthoPlate(™) ) that integrates platelet-mimetic site-selective 'adhesion' and 'aggregation' functionalities via heteromultivalent surface-decoration of lipid vesicles with Von Willebrand Factor-binding, collagen-binding and active platelet integrin GPIIb-IIIa-binding peptides...
December 7, 2016: Journal of Thrombosis and Haemostasis: JTH
https://www.readbyqxmd.com/read/27925682/influence-of-morphology-and-pbi%C3%A2-on-the-intrinsic-trap-state-distribution-in-perovskite-film-determined-by-temperature-dependent-fluorescence-spectroscopy
#7
Yi Wang, Hao-Yi Wang, Man Yu, Li-Min Fu, Yujun Qin, Jian-Ping Zhang, Xi-Cheng Ai
Perovskite films with different particle sizes and PbI₂ contents are prepared by controlled single- or sequential-method. By means of temperature dependent fluorescence spectroscopy, the energetic distribution of intrinsic intra-gap trap states in perovskite is quantitatively determined, and the radiative charge recombinations through band edge and via trap states are respectively studied. Furthermore, a series of thermodynamics parameters, such as the demarcation energy between radiative and non-radiative recombination regions, detrapping activation energy and characteristic temperature, are extracted out, based on which, the possible radiative and non-radiative recombination mechanisms are proposed...
December 7, 2016: Chemphyschem: a European Journal of Chemical Physics and Physical Chemistry
https://www.readbyqxmd.com/read/27925668/cyclic-immunofluorescence-cycif-a-highly-multiplexed-method-for-single-cell-imaging
#8
Jia-Ren Lin, Mohammad Fallahi-Sichani, Jia-Yun Chen, Peter K Sorger
Cyclic Immunofluorescence (CycIF) is a public-domain method for performing highly multiplexed immunofluorescence imaging using a conventional epifluorescence microscope. It uses simple reagents and existing antibodies to construct images with up to 30 channels by sequential 4- to 6-channel imaging followed by fluorophore inactivation. Three variant methods are described, the most generally useful of which involves staining fixed cells with antibodies directly conjugated to Alexa Fluor dyes and imaging in four colors, inactivating fluorophores using a mild base in the presence of hydrogen peroxide and light, and then performing another round of staining and imaging...
December 7, 2016: Current Protocols in Chemical Biology
https://www.readbyqxmd.com/read/27925667/tracking-the-activity-of-mtorc1-in-living-cells-using-genetically-encoded-fret-based-biosensor-torcar
#9
Xin Zhou, Simin Li, Jin Zhang
Mechanistic target of rapamycin complex 1 (mTORC1) is a highly conserved serine/threonine protein kinase that responds to multiple distinct signals (e.g., growth factors, amino acids, stress, and energy level) and coordinates cell growth and proliferation. The underlying molecular mechanisms by which these stimuli regulate the activity of mTORC1 are still not fully understood. The spatial compartmentalization of mTORC1 signaling has been suggested as an important mechanism for mTORC1 to achieve the signal specificity and efficiency...
December 7, 2016: Current Protocols in Chemical Biology
https://www.readbyqxmd.com/read/27925463/programmed-assembly-of-host-guest-protein-crystals
#10
Thaddaus R Huber, Luke F Hartje, Eli C McPherson, Ann E Kowalski, Christopher D Snow
The binding and release of guest fluorescent proteins inside a protein crystal with 13 nm axial pores is controlled. Spatially segregated guest protein loading is achieved via sequential binding and release stages. Additionally, selective stabilization of the crystal exterior results in hollow crystalline shells.
December 7, 2016: Small
https://www.readbyqxmd.com/read/27925460/nir-mediated-nanohybrids-of-upconversion-nanophosphors-and-fluorescent-conjugated-polymers-for-high-efficiency-antibacterial-performance-based-on-fluorescence-resonance-energy-transfer
#11
Junting Li, Qi Zhao, Feng Shi, Chenghui Liu, Yanli Tang
A novel nanohybrid comprised of upconversion nanophosphors (UCNPs) and fluorescent conjugated polymers (PFVCN) is rationally fabricated. The new UCNP/PFVCN nanohybrids combine the excellent antibacterial ability of PFVCN and the near IR (NIR) absorbing property of UCNPs, which allows for NIR-mediated antibacterial through the effective fluorescence resonance energy transfer from UCNPs to PFVCN accompanied with generation of reactive oxygen species to kill bacteria.
December 2016: Advanced Healthcare Materials
https://www.readbyqxmd.com/read/27925417/effects-of-temperature-on-multiparametric-evaluation-of-hindlimb-ischemia-with-dynamic-fluorescence-imaging
#12
Wenjuan Cai, Huizhi Guang, Chuangjian Cai, Jianwen Luo
Quantitative evaluation of hindlimb ischemia is essential for early diagnosis and therapy of peripheral arterial disease (PAD). Dynamic imaging using near-infrared (NIR) fluorophore indocyanine green (ICG) is a noninvasive and effective tool to monitor multiple vascular parameters including perfusion rate (PR), perfusion vascular density (PVD) and hemodynamics. It has been previously demonstrated that temperature changes could lead to significant variations of blood flow rate and vascular perfusion. In this paper, multiparametric evaluation of hindlimb ischemia was performed at different temperatures...
December 7, 2016: Journal of Biophotonics
https://www.readbyqxmd.com/read/27925284/extent-of-agreement-between-the-body-fluid-model-of-sysmex-xn-20-and-the-manual-microscopy-method
#13
Wei-Hua Huang, Lin-Peng Lu, Kang Wu, Fang-Yu Guo, Jie Guo, Jing-Long Yu, Dao-Yin Zhou, Yi Sun, An-Mei Deng
BACKGROUND: Although the correlations concerning cellular component analysis between the Sysmex XN-20 body fluid (BF) model and manual microscopy have been investigated by several studies, the extent of agreement between these two methods has not been investigated. METHODS: A total of 90 BF samples were prospectively collected and analyzed using the Sysmex XN-20 BF model and microscopy. The extent of agreement between these two methods was evaluated using the Bland-Altman approach...
December 7, 2016: Journal of Clinical Laboratory Analysis
https://www.readbyqxmd.com/read/27925281/effect-of-natural-organic-matter-on-the-photo-induced-toxicity-of-titanium-dioxide-tio2-nanoparticles
#14
Alexis M Wormington, Jason Coral, Matthew M Alloy, Carmen L Damare, Charles M Mansfield, Stephen J Klaine, Joseph H Bisesi, Aaron P Roberts
Nano-TiO2 is the most widely used form of nanoparticles in commercial industry, and come in two main configurations: rutile and anatase. Rutile TiO2 is used in UV screening applications, while anatase TiO2 crystals have a surface defect that makes them photoreactive. There are numerous reports in the literature of photo-induced toxicity to aquatic organisms following co-exposure to anatase nano-TiO2 and UV. All natural freshwater contains varying amounts of natural organic matter (NOM), which can drive UV attenuation and quench ROS in aquatic ecosystems...
December 7, 2016: Environmental Toxicology and Chemistry
https://www.readbyqxmd.com/read/27925277/identification-of-sources-of-metal-in-the-agricultural-soils-of-guanzhong-plain-northwest-china
#15
Wenbo Deng, Xuxiang Li, Zhisheng An, Liu Yang, Kang Hou, Yue Zhang
In this study, we conducted a comprehensive survey to assess the possible sources and distribution of metals in the agricultural soils of Guanzhong Plain, northwest China. A total of 227 samples of agricultural soil were collected to determine the content of Pb, Cr, Co, V, Ni, Zn, Cu, As and Mn by X-ray fluorescence spectrometry. The mean concentrations of metals were 26.2 ± 10.8 mg · kg(-1) , 71.5 ± 16.8 mg · kg(-1) , 12.7 ± 1.81 mg · kg(-1) , 85.1 ± 7.86 mg · kg(-1) , 30...
December 7, 2016: Environmental Toxicology and Chemistry
https://www.readbyqxmd.com/read/27925224/fluorescent-protein-photoprotein-fusions-and-their-applications-in-calcium-imaging
#16
Adil Bakayan, Beatriz Domingo, Cecilia F Vaquero, Nadine Peyriéras, Juan Llopis
Calcium-activated photoproteins, such as aequorin, have been used as luminescent Ca(2+) indicators since 1967. After the cloning of aequorin in 1985, microinjection was substituted by its heterologous expression, which opened the way for a widespread use. Molecular fusion of GFP to aequorin recapitulated the nonradiative energy transfer process that occurs in the jellyfish Aequorea victoria, from which these two proteins were obtained, resulting in an increase of light emission and a shift to longer wavelength...
December 7, 2016: Photochemistry and Photobiology
https://www.readbyqxmd.com/read/27925068/occurrence-of-neospora-caninum-and-toxoplasma-gondii-antibodies-in-dogs-from-rural-properties-surrounding-a-biological-reserve-espirito-santo-brasil
#17
Igor da Cunha Lima Acosta, Luana D'Avila Centoducatte, Herbert Sousa Soares, Arlei Marcili, Maria Fernanda Naegeli Gondim, João Luiz Rossi, Solange Maria Gennari
The aim of this study was to determine the presence of antibodies to Neospora caninum and Toxoplasma gondii in serum samples of 187 dogs from 30 rural properties surrounding Córrego do Veado Biological Reserve, Espírito Santo State, Brazil. The Reserve is one of the last lowland Atlantic Forest remnants of the region, surrounded by agriculture farms and cattle pastures. The presence of IgG antibodies was determined by the Indirect Fluorescent Antibody Test for T. gondii (cut-off 1:16) and N. caninum (cut-off 1:50)...
December 1, 2016: Revista Brasileira de Parasitologia Veterinária, Brazilian Journal of Veterinary Parasitology
https://www.readbyqxmd.com/read/27925020/susceptibility-of-phytomonas-serpens-to-calpain-inhibitors-in-vitro-interference-on-the-proliferation-ultrastructure-cysteine-peptidase-expression-and-interaction-with-the-invertebrate-host
#18
Simone Santiago Carvalho de Oliveira, Diego de Souza Gonçalves, Aline Dos Santos Garcia-Gomes, Inês Correa Gonçalves, Sergio Henrique Seabra, Rubem Figueiredo Menna-Barreto, Angela Hampshire de Carvalho Santos Lopes, Claudia Masini D'Avila-Levy, André Luis Souza Dos Santos, Marta Helena Branquinha
A pleiotropic response to the calpain inhibitor MDL28170 was detected in the tomato parasite Phytomonas serpens. Ultrastructural studies revealed that MDL28170 caused mitochondrial swelling, shortening of flagellum and disruption of trans Golgi network. This effect was correlated to the inhibition in processing of cruzipain-like molecules, which presented an increase in expression paralleled by decreased proteolytic activity. Concomitantly, a calcium-dependent cysteine peptidase was detected in the parasite extract, the activity of which was repressed by pre-incubation of parasites with MDL28170...
December 1, 2016: Memórias do Instituto Oswaldo Cruz
https://www.readbyqxmd.com/read/27925019/entering-and-exiting-behaviour-of-the-phlebotomine-sand-fly-lutzomyia-longiflocosa-diptera-psychodidae-in-rural-houses-of-the-sub-andean-region-of-colombia
#19
Raúl Hernando Pardo, Erika Santamaría, Olga Lucia Cabrera
The present study identified the entering and exiting sites for Lutzomyia longiflocosa in rural houses of the sub-Andean region in Colombia. Entering sites were identified with sticky traps set up outside the bedrooms, around the eave openings, and with cage traps enclosing the slits in the doors and windows inside the bedrooms. Exiting sites were identified by releasing groups of females indoors. These females were blood fed and marked with fluorescent powders. Females were recaptured with the trap placement described above but set up on the opposite sides of the openings...
December 1, 2016: Memórias do Instituto Oswaldo Cruz
https://www.readbyqxmd.com/read/27924984/infrared-imaging-of-high-density-protein-arrays
#20
Joëlle De Meutter, Julie Vandenameele, André Matagne, Erik Goormaghtigh
We propose in this paper that protein microarrays could be analysed by infrared imaging in place of enzymatic or fluorescence labelling. This label-free method reports simultaneously a large series of data on the spotted sample (protein secondary structure, phosphorylation, glycosylation, presence of impurities, etc.). In the present work, 100 μm protein spots each containing about 100 pg protein were deposited to form high density regular arrays. Using arrays of infrared detectors, high resolution images could be obtained where each pixel of the image is in fact a full infrared spectrum...
December 7, 2016: Analyst
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