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David Fitzpatrick, Michael McKenna, Edward A S Duncan, Colville Laird, Richard Lyon, Alasdair Corfield
BACKGROUND: Poor communication during patient handover is recognised internationally as a root cause of a significant proportion of preventable deaths. Improving the accuracy and quality of handover may reduce associated mortality and morbidity. Although the practice of handover between Ambulance and Emergency Department clinicians has received some attention over recent years there is little evidence to support handover best practice within the prehospital domain. Further research is therefore urgently required to understand the most appropriate way to deliver clinical information exchange in the pre-hospital environment...
June 1, 2018: Scandinavian Journal of Trauma, Resuscitation and Emergency Medicine
Tobias Thuile, Lorenz Larcher, Barbara Gatscher, Karl Schwaiger, Jenny Deluca, Amgiad Fallaha, Lukas Tappeiner, Mario Puviani, Klaus Eisendle
HINTERGRUND UND ZIELE: Die Rekonstruktion nach Entfernung ausgedehnter maligner Hauttumoren am Ohr ist eine Herausforderung. Verschiedene Methoden der Defektdeckung wurden beschrieben. Lappenplastiken, längere Anästhesiezeiten, geringe Compliance und Antikoagulation stellen bei den oft älteren Patienten zusätzliche Risiken dar. Eine rasch, effizient und unkompliziert durchführbare Methode der Defektdeckung ist daher wünschenswert. Ziel der Studie war die Beurteilung des ästhetischen Resultats, des Heilungsprozesses, der Komplikations- und Rezidivrate nach Transplantation ungenetzter Spalthaut (SPHT) zur Defektdeckung nach knorpelerhaltender Resektion maligner Tumoren am Ohr...
February 2018: Journal der Deutschen Dermatologischen Gesellschaft, Journal of the German Society of Dermatology: JDDG
Yinglan Su, Zhongjun Zhang, Qiuli Zhang, Yaoxian Zhang, Zhanli Liu
PURPOSE: Secondary hyperparathyroidism (SHPT) develops in patients with chronic renal failure. This study aimed to compare analgesic efficacy in SHPT patients who undergo subtotal parathyroidectomy after superficial versus deep cervical plexus block. METHODS: Sixty chronic renal failure patients with SPHT scheduled for subtotal parathyroidectomy were randomized to receive general anesthesia (group GA), general anesthesia plus bilateral superficial and deep cervical plexus block (group BD), or general anesthesia plus bilateral superficial cervical plexus block (group BS) (n = 20)...
December 2015: Annals of Surgical Treatment and Research
Prabhu Durai, Arulvasu Chinnasamy, Babu Gajendran, Manikandan Ramar, Srinivasan Pappu, Govindaraju Kasivelu, Ashokkumar Thirunavukkarasu
Metallic nanoparticles are major concern, particularly silver nanoparticles (AgNPs) are used in various applications. In the present investigation, we report a novel strategy with biological approach for synthesis of AgNPs using sodium para-hydroxybenzoate tetrahydrate (SPHT) isolated from Vitex negundo leaves. The synthesized SPHT-AgNPs were characterized by UV-vis spectroscopy, high resolution transmission electron microscopy (HRTEM) with selected area electron diffraction (SAED) pattern, field emission scanning electron microscopy (FESEM) with energy-dispersive X-ray spectroscopy (EDX), zeta potential and Fourier transform infrared spectroscopy (FT-IR) analysis...
September 12, 2014: European Journal of Medicinal Chemistry
Rasapirose Somwatcharajit, Itsares Tiantad, Watanalai Panbangred
The unexpressed cry2Ab27 gene of Bacillus thuringiensis subsp. aizawai SP41 (SP41) consists of a single open reading frame (ORF) of 1902bp encoding for 634 amino acid residues. The cry2Ab27 gene appears to be silent due to the lack of promoter and terminator sequences. In this study we fused the cry2Ab27 ORF with the cry1Ab promoter (500bp) and the terminator (300bp) in vector pHT304-18Z in order to drive the expression of cry2Ab27 in both SP41 and an acrystaliferous, B. thuringiensis subsp. thuringiensis 407 (407)...
February 2014: Journal of Invertebrate Pathology
Sun Ryoung Choi, Ji Hee Lim, Min Young Kim, Yu-Ah Hong, Byung Ha Chung, Sungjin Chung, Bum Soon Choi, Chul Woo Yang, Yong-Soo Kim, Yoon Sik Chang, Cheol Whee Park
BACKGROUND: Secondary hyperparathyroidism (SHPT) in patients on hemodialysis is strongly associated with cardio-vascular morbidity and mortality. Treatment of SHPT with cinacalcet decreases circulating parathyroid hormone (PTH) concentrations and lowers serum calcium and phosphorus concentrations. Therefore, we investigated the cardiovascular effects of cinacalcet in hemodialysis patients with SHPT. METHODS: We studied 12 hemodialysis patients with SPHT [serum intact PTH (iPTH) >300 pg/ml]...
2012: Nephron. Clinical Practice
Glen T Schumock, Dennis L Andress, Steven E Marx, Raimund Sterz, Amie T Joyce, Kamyar Kalantar-Zadeh
BACKGROUND/AIMS: The objective of this study was to examine health care costs and utilization and the risks of dialysis or mortality among diabetic predialysis chronic kidney disease (CKD) patients with and without secondary hyperparathyroidism (SHPT). METHODS: This retrospective, matched cohort study examined insurance claims from 703 adult diabetic predialysis CKD patients with and without SHPT during a 72-month follow-up period. Annualized estimates of health care service utilization, costs and disease progression to dialysis or death following index CKD diagnosis were compared...
2009: Nephron. Clinical Practice
L Øvreås, F L Daae, V Torsvik, F Rodríguez-Valera
The diversity of prokaryotes inhabiting solar saltern ponds was determined by thermal melting and reassociation of community DNA. These measurements were compared with fingerprinting techniques such as terminal restriction fragment length polymorphisms (T-RFLP) analysis, denaturant gradient gel electrophoresis (DGGE), and cloning and sequencing approaches. Three ponds with salinities of 22, 32, and 37% (NaCl saturation) were studied. The combination of independent molecular techniques to estimate the total genetic diversity provided a realistic assessment to reveal the microbial diversity in these environments...
October 2003: Microbial Ecology
Susana Benlloch, Arantxa López-López, Emilio O Casamayor, Lise Øvreås, Victoria Goddard, Frida Lise Daae, Gary Smerdon, Ramón Massana, Ian Joint, Frede Thingstad, Carlos Pedrós-Alió, Francisco Rodríguez-Valera
Bacterial and archaeal assemblages have been studied in a multipond solar saltern using a range of microbial ecology techniques by four laboratories simultaneously. These include 16S rDNA sequencing from both denaturing gradient gel electrophoresis (DGGE) and clone libraries, and culturing methods. Water samples from eight ponds were analysed, covering a salinity range from near sea water (4% salt) to saturated sodium chloride (37% salt; ponds called crystallizers). Clone libraries focused on ponds with salinity of 8%, 22% and 32%...
June 2002: Environmental Microbiology
S. Benlloch, S.G. Acinas, J. Antón, A. López-López, S.P. Luz, F. Rodríguez-Valera
The culturable haloarchaeal diversity in a crystallizer pond from a solar saltern has been analyzed and compared with the biodiversity directly retrieved by analysis of rRNA genes amplified from the environment. Two different sets of culture conditions have been assayed: solid medium with yeast extract as carbon source and liquid media with either yeast extract or a mixture of fishmeal, Spirulina sp., and Artemia salina. Seventeen colonies grown on plates with yeast extract incubated at 30 degrees C were analyzed by 16S rDNA partial sequencing...
January 2001: Microbial Ecology
J Antón, E Llobet-Brossa, F Rodríguez-Valera, R Amann
A fluorescence in situ hybridization (FISH) protocol suitable for the identification of prokaryotes inhabiting hypersaline environments was developed and applied to several crystallizer ponds with salinities above 36% from a multipond solar saltern in Alicante, Spain. Two morphotypes were abundant in these environments: rods and square or square-like prokaryotes that could be affiliated to Bacteria and Archaea, respectively, by FISH with domain-specific probes. FISH with a newly designed probe proved that the archaeal 16S rDNA sequence most frequently recovered from the crystallizers, SPhT, originated from the dominant square-like prokaryotes...
December 1999: Environmental Microbiology
F Cetta
The possible formation of brown recurrent common duct stones (RCS) as a long-term side effect of sphincterotomy (SPHT) has been evaluated in 63 patients with stone formation after cholecystectomy, 253 who underwent SPHT or choledocholithotomy, 131 with postoperative monitoring of bile bacteriologic characteristics through the T tube, and 20 with stone and bile analysis at both operations. In addition, findings are also reported in 145 patients who underwent surgical SPHT and radiologic review of up to 28 years after surgery, five who underwent ampullectomy, and 55 who underwent endoscopic SPHT...
March 1993: Archives of Surgery
U B Noelpp, H Roesler, E P Ritter, H Ledermann
OBJECTIVE: Seven pinhole tomography (SPHT) is fast, inexpensive, and, because it relies on standard gamma cameras and computers, available everywhere. This article investigates the suitability of SPHT for the detection and localization of 511 keV gammas emitted from 18F without coincidence detection. MATERIALS AND METHODS: A standard SPH collimator and the table were additionally shielded with lead. For comparison, point sources and cardiac phantoms were imaged on SPHT and a triple head gamma camera (THC)...
January 1994: Journal of Computer Assisted Tomography
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