keyword
MENU ▼
Read by QxMD icon Read
search

inversion probes

keyword
https://www.readbyqxmd.com/read/28448963/paradoxical-aging-in-hiv-immune-senescence-of-b-cells-is-most-prominent-in-young-age
#1
Stefano Rinaldi, Suresh Pallikkuth, Varghese K George, Lesley R de Armas, Rajendra Pahwa, Celeste M Sanchez, Maria Fernanda Pallin, Li Pan, Nicola Cotugno, Gordon Dickinson, Allan Rodriguez, Margaret Fischl, Maria Alcaide, Louis Gonzalez, Paolo Palma, Savita Pahwa
Combination antiretroviral therapies (cART)can lead to normal life expectancy in HIV-infected persons, and people aged >50 yrs represent the fastest growing HIV group. Although HIV and aging are independently associated with impaired humoral immunity, immune status in people aging with HIV is relatively unexplored. In this study influenza vaccination was used to probe age associated perturbations in the B cell compartment of HIV-negative "healthy controls" (HC) and virologically controlled HIV-infected participants on cART (HIV) (n=124), grouped by age as young (<40 yrs), middle-aged (40-59yrs) or old (>60 yrs)...
April 27, 2017: Aging
https://www.readbyqxmd.com/read/28445943/a-molecular-inversion-probe-based-next-generation-sequencing-panel-to-detect-germline-mutations-in-chinese-early-onset-colorectal-cancer-patients
#2
Junxiao Zhang, Xiaoyan Wang, Richarda M de Voer, Jayne Y Hehir-Kwa, Eveline J Kamping, Robbert D A Weren, Marcel Nelen, Alexander Hoischen, Marjolijn J L Ligtenberg, Nicoline Hoogerbrugge, Xiangling Yang, Zihuan Yang, Xinjuan Fan, Lei Wang, Huanliang Liu, Jianping Wang, Roland P Kuiper, Ad Geurts van Kessel
The currently known Mendelian colorectal cancer (CRC) predisposition syndromes account for ~5-10% of all CRC cases, and are caused by inherited germline mutations in single CRC predisposing genes. Using molecular inversion probes (MIPs), we designed a targeted next-generation sequencing panel to identify mutations in seven CRC predisposing genes: APC, MLH1, MSH2, MSH6, PMS2, MUTYH and NTHL1. From a consecutive series of 2,371 Chinese CRC patients, 140 familial and non-familial cases were selected that were diagnosed with CRC at or below the age of 35 years...
April 11, 2017: Oncotarget
https://www.readbyqxmd.com/read/28441328/a-gradient-field-pulsed-eddy-current-probe-for-evaluation-of-hidden-material-degradation-in-conductive-structures-based-on-lift-off-invariance
#3
Yong Li, Haoqing Jing, Ilham Mukriz Zainal Abidin, Bei Yan
Coated conductive structures are widely adopted in such engineering fields as aerospace, nuclear energy, etc. The hostile and corrosive environment leaves in-service coated conductive structures vulnerable to Hidden Material Degradation (HMD) occurring under the protection coating. It is highly demanded that HMD can be non-intrusively assessed using non-destructive evaluation techniques. In light of the advantages of Gradient-field Pulsed Eddy Current technique (GPEC) over other non-destructive evaluation methods in corrosion evaluation, in this paper the GPEC probe for quantitative evaluation of HMD is intensively investigated...
April 25, 2017: Sensors
https://www.readbyqxmd.com/read/28438365/protein-conformational-dynamics-studied-by-15-n-and-1-h-r1%C3%AF-relaxation-dispersion-application-to-wild-type-and-g53a-ubiquitin-crystals
#4
Diego F Gauto, Audrey Hessel, Petra Rovó, Vilius Kurauskas, Rasmus Linser, Paul Schanda
Solid-state NMR spectroscopy can provide site-resolved information about protein dynamics over many time scales. Here we combine protein deuteration, fast magic-angle spinning (~45-60kHz) and proton detection to study dynamics of ubiquitin in microcrystals, and in particular a mutant in a region that undergoes microsecond motions in a β-turn region in the wild-type protein. We use (15)N R1ρ relaxation measurements as a function of the radio-frequency (RF) field strength, i.e. relaxation dispersion, to probe how the G53A mutation alters these dynamics...
April 14, 2017: Solid State Nuclear Magnetic Resonance
https://www.readbyqxmd.com/read/28432143/focal-local-field-potential-lfp-signature-of-the-single-axon-monosynaptic-thalamocortical-connection
#5
Espen Hagen, Janne C Fossum, Klas H Pettersen, Jose-Manuel Alonso, Harvey A Swadlow, Gaute T Einevolla
Recent years have seen a resurgence in use of the extracellularly recorded local field potential (LFP) to investigate neural network activity. To probe monosynaptic thalamic activation of cortical postsynaptic target cells, so called spike-trigger-averaged LFP (stLFP) signatures have been measured. In these experiments the cortical LFP is measured by means of multielectrodes covering several cortical lamina and averaged on spontaneous spikes of thalamocortical (TC) cells. Using a well-established forward-modeling scheme, we investigate the biophysical origin of this stLFP signature with simultaneous synaptic activation of cortical layer 4 neurons, mimicking the effect of a single afferent spike from a single TC neuron...
April 21, 2017: Journal of Neuroscience: the Official Journal of the Society for Neuroscience
https://www.readbyqxmd.com/read/28431057/quantitative-analysis-of-pkp2-and-neighbouring-genes-in-a-patient-with-arrhythmogenic-right-ventricular-cardiomyopathy-caused-by-heterozygous-pkp2-deletion
#6
Keiko Sonoda, Seiko Ohno, Sou Otuki, Koichi Kato, Nobue Yagihara, Hiroshi Watanabe, Takeru Makiyama, Tohru Minamino, Minoru Horie
Aims: Arrhythmogenic right ventricular cardiomyopathy (ARVC) is a disease mainly caused by desmosome gene mutations. The genetic culprit, however, remains elusive in ∼50% of ARVC patients. One of the reasons for missing genetic abnormalities is the difficulty in detecting large deletions/duplications, which are called as copy number variation (CNV) by the Sanger sequencing method. This study aimed to identify CNVs in PKP2 and a part of other desmosome genes in ARVC patients. Methods and Results: The study cohort consisted of 71 ARVC probands who were diagnosed as definite or borderline cases based on 2010 Task Force Criteria...
April 1, 2017: Europace: European Pacing, Arrhythmias, and Cardiac Electrophysiology
https://www.readbyqxmd.com/read/28429646/rule-violations-sensitise-towards-negative-and-authority-related-stimuli
#7
Robert Wirth, Anna Foerster, Hannah Rendel, Wilfried Kunde, Roland Pfister
Rule violations have usually been studied from a third-person perspective, identifying situational factors that render violations more or less likely. A first-person perspective of the agent that actively violates the rules, on the other hand, is only just beginning to emerge. Here we show that committing a rule violation sensitises towards subsequent negative stimuli as well as subsequent authority-related stimuli. In a Prime-Probe design, we used an instructed rule-violation task as the Prime and a word categorisation task as the Probe...
April 21, 2017: Cognition & Emotion
https://www.readbyqxmd.com/read/28426926/sorbent-material-characterization-using-in-tube-extraction-needles-as-inverse-gas-chromatography-column
#8
Xochitli L Osorio Barajas, Maik A Jochmann, Thorsten Hüffer, Beat Schilling, Torsten C Schmidt
In-tube extraction is a full automated enrichment technique, which consists of a stainless-steel needle, packed with sorbent material for the extraction of volatile and semi-volatile compounds. In principle, all particulate sorbents used for enrichment in air or headspace analysis can be used. However, the selection of the sorbents is merely based on empirical considerations rather than on experimental data, which is caused by a lack of knowledge about the relevant physicochemical properties of the sorbent...
April 20, 2017: Journal of Separation Science
https://www.readbyqxmd.com/read/28426149/click-chemistry-mediated-rapid-microbubble-catching-for-acute-thrombus-ultrasound-molecular-imaging
#9
Tuantuan Wang, Chuxiao Yuan, Bingyang Dai, Yang Liu, Mingxi Li, Zhenqiang Feng, Qing Jiang, Zhihong Xu, Ningwei Zhao, Ning Gu, Fang Yang
Bioorthogonal coupling chemistry has been studied as one significant advantage for molecular imaging as it offers rapid, efficient, and strong binding, which may also benefit in stability, production, and chemical conjugation. The inverse-electron-demand Diels-Alder reaction between s-tetrazine and trans-cyclooctene (TCO) is an example of a highly selective and rapid bioorthogonal coupling reaction to be used successfully to prepare targeted molecular imaging probes. Herein, based on a two-step pretargeting bioorthogonal chemistry, we report a fast and reliable highly sensitive approach to achieve activated-platelet-specific CD62p targeted thrombus ultrasound molecular imaging...
April 20, 2017: Chembiochem: a European Journal of Chemical Biology
https://www.readbyqxmd.com/read/28424513/super-multiplex-vibrational-imaging
#10
Lu Wei, Zhixing Chen, Lixue Shi, Rong Long, Andrew V Anzalone, Luyuan Zhang, Fanghao Hu, Rafael Yuste, Virginia W Cornish, Wei Min
The ability to visualize directly a large number of distinct molecular species inside cells is increasingly essential for understanding complex systems and processes. Even though existing methods have successfully been used to explore structure-function relationships in nervous systems, to profile RNA in situ, to reveal the heterogeneity of tumour microenvironments and to study dynamic macromolecular assembly, it remains challenging to image many species with high selectivity and sensitivity under biological conditions...
April 19, 2017: Nature
https://www.readbyqxmd.com/read/28418642/structure-and-dynamics-of-individual-diastereomeric-complexes-on-platinum-surface-studies-related-to-heterogeneous-enantioselective-catalysis
#11
Yi Dong, Guillaume Goubert, Michael N Groves, Jean-Christian Lemay, Bjørk Hammer, Peter H McBreen
The modification of heterogeneous catalysts through the chemisorption of chiral molecules is a method to create catalytic sites for enantioselective surface reactions. The chiral molecule is called a chiral modifier by analogy to the terms chiral auxiliary or chiral ligand used in homogeneous asymmetric catalysis. While there has been progress in understanding how chirality transfer occurs, the intrinsic difficulties in determining enantioselective reaction mechanisms are compounded by the multisite nature of heterogeneous catalysts and by the challenges facing stereospecific surface analysis...
April 18, 2017: Accounts of Chemical Research
https://www.readbyqxmd.com/read/28416565/cingulo-insular-structural-alterations-associated-with-psychogenic-symptoms-childhood-abuse-and-ptsd-in-functional-neurological-disorders
#12
David L Perez, Nassim Matin, Arthur Barsky, Victor Costumero-Ramos, Sara J Makaretz, Sigrid S Young, Jorge Sepulcre, W Curt LaFrance, Matcheri S Keshavan, Bradford C Dickerson
OBJECTIVE: Adverse early-life events are predisposing factors for functional neurological disorder (FND) and post-traumatic stress disorder (PTSD). Cingulo-insular regions are implicated in the biology of both conditions and are sites of stress-mediated neuroplasticity. We hypothesised that functional neurological symptoms and the magnitude of childhood abuse would be associated with overlapping anterior cingulate cortex (ACC) and insular volumetric reductions, and that FND and PTSD symptoms would map onto distinct cingulo-insular areas...
April 17, 2017: Journal of Neurology, Neurosurgery, and Psychiatry
https://www.readbyqxmd.com/read/28414424/quantifying-the-hierarchical-order-in-self-aligned-carbon-nanotubes-from-atomic-to-micrometer-scale
#13
Eric R Meshot, Darwin W Zwissler, Ngoc Bui, Tevye R Kuykendall, Cheng Wang, Alexander Hexemer, Kuang Jen J Wu, Francesco Fornasiero
Fundamental understanding of structure-property relationships in hierarchically organized nanostructures is crucial for the development of new functionality, yet quantifying structure across multiple length scales is challenging. In this work, we used nondestructive X-ray scattering to quantitatively map the multiscale structure of hierarchically self-organized carbon nanotube (CNT) "forests" across 4 orders of magnitude in length scale, from 2.0 Å to 1.5 μm. Fully resolved structural features include the graphitic honeycomb lattice and interlayer walls (atomic), CNT diameter (nano), as well as the greater CNT ensemble (meso) and large corrugations (micro)...
April 27, 2017: ACS Nano
https://www.readbyqxmd.com/read/28406614/vinyluridine-as-a-versatile-chemoselective-handle-for-the-posttranscriptional-chemical-functionalization-of-rna
#14
Jerrin Thomas George, Seergazhi G Srivatsan
Development of modular and efficient methods to functionalize RNA with biophysical probes is very important to advance the understanding of the structural and functional relevance of RNA in various cellular events. Herein we demonstrate a two-step bioorthogonal chemical functionalization approach for the conjugation of multiple probes onto RNA transcripts using a 5-vinyl-modified uridine nucleotide analog (VUTP). VUTP, containing a structurally non-invasive and versatile chemoselective handle, was efficiently incorporated into RNA transcripts by in vitro transcription reactions...
April 13, 2017: Bioconjugate Chemistry
https://www.readbyqxmd.com/read/28392627/triarylmethyl-radical-epr-signal-to-noise-at-frequencies-between-250-mhz-and-1-5-ghz-and-dependence-of-relaxation-on-radical-and-salt-concentration-and-on-frequency
#15
Yilin Shi, Richard W Quine, George A Rinard, Laura Buchanan, Sandra S Eaton, Gareth R Eaton, Boris Epel, Simone Wanless Seagle, Howard J Halpern
In vivo oximetry by pulsed electron paramagnetic resonance is based on measurements of changes in electron spin relaxation rates of probe molecules, such as the triarylmethyl radicals. A series of experiments was performed at frequencies between 250 MHz and 1.5 GHz to assist in the selection of an optimum frequency for oximetry. Electron spin relaxation rates for the triarylmethyl radical OX063 as a function of radical concentration, salt concentration, and resonance frequency were measured by electron spin echo 2-pulse decay and 3-pulse inversion recovery in the frequency range of 250 MHz-1...
April 2017: Zeitschrift Für Physikalische Chemie
https://www.readbyqxmd.com/read/28391836/fluorescence-growth-of-self-polymerized-fluorescence-polydopamine-for-ratiometric-visual-detection-of-da
#16
Miao Yu, Yang Lu, Zhenjiang Tan
In this work, a novel and facile ratiometric fluorescence probe was prepared for the visual detection of dopamine (DA). In this detection system, red-emission CdTe@SiO2 (r-QDs@SiO2) was used as steady core of the probe and inverse microemulsion method was applied to synthesize uniform r-QDs@SiO2, this step could protect CdTe from contacting with human skin directly. Polydopamine (PDA) acted as response signal to detect DA, a very handy method which just combined polyethyleneimine (PEI) with DA together to synthesize PDA, this way for synthesis of PDA was much time-saving and non-toxic than any other methods...
June 1, 2017: Talanta
https://www.readbyqxmd.com/read/28387924/high-number-of-kinome-mutations-in-non-small-cell-lung-cancer-is-associated-with-reduced-immune-response-and-poor-relapse-free-survival
#17
Å Helland, O T Brustugun, S Nakken, A R Halvorsen, T Dønnem, R Bremnes, L T Busund, J Sun, S Lorenz, S K Solberg, L H Jørgensen, D Vodak, O Myklebost, E Hovig, L A Meza-Zepeda
Lung cancer is the leading cause of cancer related death, and the past years' improved insight into underlying molecular events has significantly improved outcome for specific subsets of patients. In particular, several new therapies that target protein kinases have been implemented, and many more are becoming available. We have investigated lung cancer specimens for somatic mutations in a targeted panel of 612 human genes, the majority being protein kinases. The somatic mutation profiles were correlated to profiles of immune cell infiltration as well as relapse-free survival...
April 7, 2017: International Journal of Cancer. Journal International du Cancer
https://www.readbyqxmd.com/read/28381166/downregulation-of-microrna-181d-had-suppressive-effect-on-pancreatic-cancer-development-through-inverse-regulation-of-knain2
#18
Guopeng Zhang, Dongbo Liu, Guoxian Long, Lei Shi, Hong Qiu, Guangyuan Hu, Guoqing Hu, Shunfang Liu
We explored the expression and function of miR-181d (microRNA-181d) in human pancreatic cancer. Quantitative real-time polymerase chain reaction was used to probe miR-181d expression in both pancreatic cancer cell lines and human pancreatic carcinoma. Pancreatic cancer cell lines, PANC-1 and AsPC-1 cells, were engineered to stably downregulate endogenous miR-181d through lentiviral transduction. The mechanistic effects of miR-181d downregulation on pancreatic cancer development were tested by proliferation, migration, fluorouracil chemosensitivity assays in vitro, and explant assay in vivo...
April 2017: Tumour Biology: the Journal of the International Society for Oncodevelopmental Biology and Medicine
https://www.readbyqxmd.com/read/28380957/development-of-a-beam-optimization-method-for-absorption-based-tomography
#19
Tao Yu, Bo Tian, Weiwei Cai
Absorption tomography is an imaging technique that has been used simultaneously to image multiple scalar parameters, such as temperature and species concentration for combustion diagnostics. Practical combustors, such as internal combustion engines and gas turbine engines, only allow limited optical access, and typically a few (ca. 20-40) beams are available to probe the domain of interest. With such limited spatial sampling, it is non-trivial to optimize beam arrangement for a faithful reconstruction. Previous efforts on beam optimization rely on either heuristic/empirical methods lacking rigorous mathematical derivation or were derived by assuming certain prior information in the tomographic inversion...
March 20, 2017: Optics Express
https://www.readbyqxmd.com/read/28358927/aquaglyceroporin-null-trypanosomes-display-glycerol-transport-defects-and-respiratory-inhibitor-sensitivity
#20
Laura Jeacock, Nicola Baker, Natalie Wiedemar, Pascal Mäser, David Horn
Aquaglyceroporins (AQPs) transport water and glycerol and play important roles in drug-uptake in pathogenic trypanosomatids. For example, AQP2 in the human-infectious African trypanosome, Trypanosoma brucei gambiense, is responsible for melarsoprol and pentamidine-uptake, and melarsoprol treatment-failure has been found to be due to AQP2-defects in these parasites. To further probe the roles of these transporters, we assembled a T. b. brucei strain lacking all three AQP-genes. Triple-null aqp1-2-3 T. b. brucei displayed only a very moderate growth defect in vitro, established infections in mice and recovered effectively from hypotonic-shock...
March 2017: PLoS Pathogens
keyword
keyword
114152
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"