keyword
MENU ▼
Read by QxMD icon Read
search

Small membrane protein regulator

keyword
https://www.readbyqxmd.com/read/28548902/er-mitochondria-signaling-regulates-autophagy
#1
Patricia Gomez-Suaga, Sebastien Paillusson, Christopher C J Miller
The endoplasmic reticulum (ER) and mitochondria form tight functional contacts that regulate a number of key cellular processes. The formation of these contacts involves "tethering proteins" that function to recruit regions of ER to mitochondria. The integral ER protein VAPB (VAMP associated protein B and C) binds to the outer mitochondrial membrane protein, RMDN3/PTPIP51 (regulator of microtubule dynamics 3) to form one such set of tethers. Recently, we showed that the VAPB-RMDN3 tethers regulate macroautophagy/autophagy...
May 26, 2017: Autophagy
https://www.readbyqxmd.com/read/28548451/molecular-and-functional-characterization-of-the-american-cockroach-periplaneta-americana-rab5-the-first-exopterygotan-low-molecular-weight-ovarian-gtpase-during-oogenesis
#2
Mohamed Elmogy, Amr A Mohamed, Muhammad Tufail, Tomohide Uno, Makio Takeda
The small Rab GTPases are key regulators of membrane vesicle trafficking. Ovaries of Periplaneta americana (Linnaeus) (Blattodea: Blattidae) have small molecular weight GTP/ATP-binding proteins during early and late vitellogenic periods of oogenesis. However, the identification and characterization of the detected proteins have not been yet reported. Herein, we cloned a cDNA encoding Rab5 from the American cockroach, P. americana, ovaries (PamRab5). It comprises 796 bp, encoding a protein of 213 amino acid residues with a predicted molecular weight of 23...
May 26, 2017: Insect Science
https://www.readbyqxmd.com/read/28545854/transcriptome-wide-identification-of-host-genes-targeted-by-tomato-spotted-wilt-virus-derived-small-interfering-rnas
#3
Shunmugiah V Ramesh, Sarah Williams, Madhu Kappagantu, Neena Mitter, Hanu R Pappu
RNA silencing mechanism functions as a major defense against invading viruses. The caveat in the RNA silencing mechanism is that the effector small interfering RNAs (siRNAs) act on any RNA transcripts with sequence complementarity irrespective of target's origin. A subset of highly expressed viral small interfering RNAs (vsiRNAs) derived from the tomato spotted wilt virus (TSWV; Tospovirus: Bunyaviridae) genome was analyzed for their propensity to downregulate the tomato transcriptome. A total of 11898 putative target sites on tomato transcripts were found to exhibit a propensity for down regulation by TSWV-derived vsiRNAs...
May 22, 2017: Virus Research
https://www.readbyqxmd.com/read/28544137/phenotypic-identification-of-a-novel-autophagy-inhibitor-chemotype-targeting-lipid-kinase-vps34
#4
Herbert Waldmann, Lucas Robke, Luca Laraia, Marjorie A Carnero Corrales, Georgios Konstantinidis, Makoto Muroi, André Richters, Michael Winzker, Tobias Engbring, Stefano Tomassi, Nobumoto Watanabe, Hiroyuki Osada, Daniel Rauh, Yaowen Wu, Julian Engel
Autophagy is a critical regulator of cellular homeostasis and metabolism. Interference with this process is considered a new approach for the treatment of disease, in particular cancer and neurological disorders. Therefore, novel small molecule autophagy modulators are in high demand. We describe the discovery of autophinib, a potent autophagy inhibitor with a novel chemotype. Autophinib was identified by means of a phenotypic assay monitoring formation of autophagy-induced puncta indicating accumulation of lipidated cytosolic protein LC3 on the autophagosomal membrane...
May 22, 2017: Angewandte Chemie
https://www.readbyqxmd.com/read/28536001/membrane-shaping-by-actin-and-myosin-during-regulated-exocytosis
#5
REVIEW
Andreas Papadopulos
The cortical actin network in neurosecretory cells is a dense mesh of actin filaments underlying the plasma membrane. Interaction of actomyosin with vesicular membranes or the plasma membrane is vital for tethering, retention, transport as well as fusion and fission of exo- and endocytic membrane structures. During regulated exocytosis the cortical actin network undergoes dramatic changes in morphology to accommodate vesicle docking, fusion and replenishment. Most of these processes involve plasma membrane Phosphoinositides (PIP) and investigating the interactions between the actin cortex and secretory structures has become a hotbed for research in recent years...
May 20, 2017: Molecular and Cellular Neurosciences
https://www.readbyqxmd.com/read/28534368/study-on-the-molecular-mechanism-of-rac3-on-regulating-autophagy-in-human-lung-cancer-cells
#6
Xuyang Xiao, Gebang Wang, Hongxu Liu
PURPOSE: Rac3 plays an important role in regulating tumorigenesis. Autophagy plays a vital role in tumorigenesis and tumor progression. The relationship between the two remains unclear. The objective of the present study was to determine the specific molecular mechanism of intracellular Rac3 in regulating autophagy and reveal the relationship between tumor cell autophagy and apoptosis. METHODS: A laser confocal microscope was used to photograph the accumulated EGFP-MAP1LC3 spots for investigating the relationship between Rac3 and autophagy at the cellular level...
March 2017: Journal of B.U.ON.: Official Journal of the Balkan Union of Oncology
https://www.readbyqxmd.com/read/28531263/in-vivo-dynamics-of-the-cortical-actin-network-revealed-by-fast-scanning-atomic-force-microscopy
#7
Yanshu Zhang, Aiko Yoshida, Nobuaki Sakai, Yoshitsugu Uekusa, Masahiro Kumeta, Shige H Yoshimura
Together with lamellipodia and stress fibers, a dynamic network of actin filaments in the cell cortex plays a major role in the maintenance of cell morphology and motility. In contrast to lamellipodia, which have been well studied in various motile cells, the dynamics of actin filaments in the cell cortex have not yet been clarified due to a lack of proper imaging techniques. Here, we utilized high-speed atomic force microscopy for live-cell imaging and analyzed cortical actin dynamics in living cells. We successfully measured the polymerization rate and the frequency of filament synthesis in living COS-7 cells, and examined the associated effects of various inhibitors and actin-binding proteins...
May 20, 2017: Microscopy
https://www.readbyqxmd.com/read/28524857/a-defined-metabolic-state-in-pre-b-cells-governs-b-cell-development-and-is-counterbalanced-by-swiprosin-2-efhd1
#8
Merle Stein, Sebastian Dütting, Dimitrios Mougiakakos, Michael Bösl, Kristin Fritsch, Dorothea Reimer, Sophia Urbanczyk, Tobit Steinmetz, Wolfgang Schuh, Aline Bozec, Thomas H Winkler, Hans-Martin Jäck, Dirk Mielenz
B-cell development in the bone marrow comprises proliferative and resting phases in different niches. We asked whether B-cell metabolism relates to these changes. Compared to pro B and small pre B cells, large pre B cells revealed the highest glucose uptake and ROS but not mitochondrial mass, whereas small pre B cells exhibited the lowest mitochondrial membrane potential. Small pre B cells from Rag1(-/-);33.C9 μ heavy chain knock-in mice revealed decreased glycolysis (ECAR) and mitochondrial spare capacity compared to pro B cells from Rag1(-/-) mice...
May 19, 2017: Cell Death and Differentiation
https://www.readbyqxmd.com/read/28524836/effect-of-histone-deacetylase-inhibition-on-the-expression-of-multidrug-resistance-associated-protein-2-in-a-human-placental-trophoblast-cell-line
#9
Hong-Yu Duan, Dan Ma, Kai-Yu Zhou, Tao Wang, Yi Zhang, Yi-Fei Li, Jin-Lin Wu, Yi-Min Hua, Chuan Wang
BACKGROUND: Placental multidrug resistance-associated protein 2 (MRP2), encoded by ABCC2 gene in human, plays a significant role in regulating drugs' transplacental transfer rates. Studies on placental MRP2 regulation could provide more therapeutic targets for individualized and safe pharmacotherapy during pregnancy. Currently, the roles of epigenetic mechanisms in regulating placental drug transporters are still unclear. This study aimed to investigate the effect of histone deacetylases (HDACs) inhibition on MRP2 expression in the placental trophoblast cell line and to explore whether HDAC1/2/3 are preliminarily involved in this process...
June 5, 2017: Chinese Medical Journal
https://www.readbyqxmd.com/read/28522593/small-gtpase-rab1b-is-associated-with-atg9a-vesicles-and-regulates-autophagosome-formation
#10
Soichiro Kakuta, Junji Yamaguchi, Chigure Suzuki, Mitsuho Sasaki, Saiko Kazuno, Yasuo Uchiyama
ATG9 is a membrane protein that is essential for autophagy and is considered to be directly involved in the early steps of autophagosome formation. Yeast Atg9 is mainly localized to small vesicles (Atg9 vesicles), whereas mammalian ATG9A is reportedly localized to the trans-Golgi network, the endosomal compartment, and other unidentified membrane structures. To dissect the ATG9A-containing membranes, we examined the subcellular localization of ATG9A and performed immunoisolation of those membranes. ATG9A-green fluorescent protein in human culture cells was observed as numerous puncta that move rapidly throughout the cytoplasm...
May 18, 2017: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/28515293/virus-like-vesicles-of-kaposi-s-sarcoma-associated-herpesvirus-activate-lytic-replication-through-triggering-differentiation-signaling
#11
Danyang Gong, Xinghong Dai, Yuchen Xiao, Yushen Du, Travis J Chapa, Jeffrey R Johnson, Xinmin Li, Nevan J Krogan, Hongyu Deng, Ting-Ting Wu, Ren Sun
Virus-like vesicles (VLVs) are membrane enclosed vesicles that resemble native enveloped viruses in organization, but lack viral capsid and genome. During the productive infection of tumor associated gamma-herpesviruses, both virions and VLVs are produced and released into the extracellular space. However, studies of gamma-herpesvirus associated VLVs have been largely restricted by the technical difficulty of separating VLVs from mature virions. Here, we report a strategy of using a Kaposi's sarcoma-associated herpesvirus (KSHV) mutant defective in small capsid protein, unable to produce mature virions, to selectively isolate VLVs...
May 17, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28515233/wntless-sec12-complex-on-er-membrane-regulates-early-wnt-secretory-vesicle-assembly-and-mature-ligand-export
#12
Jiaxin Sun, Shiyan Yu, Xiao Zhang, Catherine Capac, Onyedikachi Aligbe, Timothy Daudelin, Edward M Bonder, Nan Gao
Wntless (Wls) transports Wnt molecules for secretion, however the cellular mechanism underlying the initial assembly of Wnt secretory vesicles is still not fully defined. Using proteomic and mutagenic analyses of the mammalian Wls, we report a mechanism for formation of early Wnt secretory vesicle on ER membrane. Wls forms a complex with SEC12, an ER membrane-localized GEF activator of SAR1 small GTPase. Compared to palmitoylation-deficient Wnt molecules, binding of mature Wnt to Wls increases Wls-SEC12 interaction and promotes Wls association with SAR1, the key activator of the COPII machinery...
May 17, 2017: Journal of Cell Science
https://www.readbyqxmd.com/read/28512220/increasing-intracellular-magnesium-levels-with-the-31-amino-acid-mgts-protein
#13
Hanbo Wang, Xuefeng Yin, Mona Wu Orr, Michael Dambach, Rebecca Curtis, Gisela Storz
Synthesis of the 31-amino acid, inner membrane protein MgtS (formerly denoted YneM) is induced by very low Mg(2+) in a PhoPQ-dependent manner in Escherichia coli Here we report that MgtS acts to increase intracellular Mg(2+) levels and maintain cell integrity upon Mg(2+) depletion. Upon development of a functional tagged derivative of MgtS, we found that MgtS interacts with MgtA to increase the levels of this P-type ATPase Mg(2+) transporter under Mg(2+)-limiting conditions. Correspondingly, the effects of MgtS upon Mg(2+) limitation are lost in a ∆mgtA mutant, and MgtA overexpression can suppress the ∆mgtS phenotype...
May 16, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28510051/proteins-in-binary-solvents
#14
REVIEW
Francesco Spinozzi, Paolo Mariani, Maria Grazia Ortore
Proteins in living organisms exist in complex aqueous solutions or embedded in membranes. In solution, proteins are surrounded by a tightly bound hydration layer, which is more ordered and less mobile than bulk water. As a consequence, water plays a major role in controlling protein structure stability, conformational flexibility, dynamics, and functionality, but it also appears that protein surface regulates the structuring of the surrounding water. The presence of cosolvents can modify the hydration layer characteristics and then the whole protein structural and dynamical properties...
June 2016: Biophysical Reviews
https://www.readbyqxmd.com/read/28508109/solvent-accessibility-of-discrete-residue-positions-in-the-polypeptide-hormone-glucagon-by-19-f-nmr-observation-of-4-fluorophenylalanine
#15
Yaguang Hou, Wanhui Hu, Xiaona Li, John J Skinner, Dongsheng Liu, Kurt Wüthrich
The amino acid 4-fluoro-L-phenylalanine (4F-Phe) was introduced at the positions of Phe6 and Phe22 in the 29-residue polypeptide hormone glucagon by expressing glucagon in E. coli in the presence of an excess of 4F-Phe. Glucagon regulates blood glucose homeostasis by interaction with the glucagon receptor (GCGR), a class B GPCR. By referencing to the 4F-Phe chemical shifts at varying D2O concentrations, the solvent exposure of the two Phe sites along the glucagon sequence was determined, showing that 4F-Phe6 was fully solvent exposed and 4F-Phe22 was only partially exposed...
May 15, 2017: Journal of Biomolecular NMR
https://www.readbyqxmd.com/read/28507245/conformational-changes-of-inter-domain-linker-mediate-mechanical-signal-transmission-in-sensor-kinase-bvgs
#16
Elodie Lesne, Elian Dupré, Camille Locht, Rudy Antoine, Françoise Jacob-Dubuisson
The whooping cough agent, Bordetella pertussis, controls the expression of its large virulence regulon in a coordinated manner through the two-component system BvgAS. BvgS is a dimeric, multi-domain sensor-kinase. Each monomer comprises in succession tandem periplasmic Venus flytrap (VFT) domains, a transmembrane segment, a cytoplasmic Per-ArnT-Sim (PAS) domain, a kinase module and additional phosphorelay domains. BvgS shifts between kinase and phosphatase modes of activity in response to chemical modulators that modify the clamshell motions of the VFT domains...
May 15, 2017: Journal of Bacteriology
https://www.readbyqxmd.com/read/28500851/calreticulin-modulates-the-intracellular-survival-of-mycobacteria-by-regulating-er-stress-mediated-apoptosis
#17
Sung Hee Jo, Ji-Ae Choi, Yun-Ji Lim, Junghwan Lee, Soo-Na Cho, Sung-Man Oh, Dam Go, Seon-Hwa Kim, Chang-Hwa Song
Endoplasmic reticulum (ER)-stress-mediated apoptosis is a host defense mechanism against Mycobacterium tuberculosis (Mtb) infection. Calreticulin (CRT) is the major calcium-binding chaperone protein. Previous reports have suggested a close relationship between the cell-surface expression of CRT and apoptosis. In this study, the role of CRT during Mtb infection was examined. The results showed that Mtb infection induces CRT production by macrophages and that CRT levels are correlated with the degree of apoptotic cell death...
April 25, 2017: Oncotarget
https://www.readbyqxmd.com/read/28494186/small-interference-itga6-gene-targeting-in-the-human-thymic-epithelium-differentially-regulates-the-expression-of-immunological-synapse-related-genes
#18
Daiane Cristina F Golbert, Eliane Santana-Van-Vliet, Marcelo Ribeiro-Alves, Marbella Maria B da Fonsêca, Ailin Lepletier, Daniella Arêas Mendes-da-Cruz, Guilherme Loss, Vinícius Cotta-de-Almeida, Ana Tereza R Vasconcelos, Wilson Savino
The thymus supports differentiation of T cell precursors. This process requires relocation of developing thymocytes throughout multiple microenvironments of the organ, mainly with thymic epithelial cells (TEC), which control intrathymic T cell differentiation influencing the formation and maintenance of the immunological synapse. In addition to the proteins of the major histocompatibility complex (MHC), this structure is supported by several adhesion molecules. During the process of thymopoiesis, we previously showed that laminin-mediated interactions are involved in the entrance of T-cell precursors into the thymus, as well as migration of differentiating thymocytes within the organ...
May 11, 2017: Cell Adhesion & Migration
https://www.readbyqxmd.com/read/28487373/interactions-between-small-ankyrin-1-and-sarcolipin-coordinately-regulate-activity-of-the-sarco-endo-plasmic-reticulum-ca2-atpase-serca1
#19
Patrick F Desmond, Amanda Labuza, Joaquin Muriel, Michele L Markwardt, Allison E Mancini, Mark A Rizzo, Robert J Bloch
SERCA1, the sarco(endo)plasmic reticulum Ca2+-ATPase of skeletal muscle, is essential for muscle relaxation and maintenance of low resting Ca2+ levels in the myoplasm. We recently reported that small ankyrin 1 (sAnk1) interacts with the sarco(endo)plasmic reticulum Ca2+-ATPase in skeletal muscle (SERCA1) to inhibit its activity (1). We also showed that this interaction is mediated at least in part through sAnk1's transmembrane domain in a manner similar to that of sarcolipin (SLN). Earlier studies have shown that SLN and phospholamban (PLN), the other well-studied small SERCA-regulatory proteins, oligomerize either alone or together...
May 9, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28483636/sublocalization-of-cytochrome-b6f-complexes-in-photosynthetic-membranes
#20
REVIEW
Helmut Kirchhoff, Meng Li, Sujith Puthiyaveetil
It is well established that the majority of energy-converting photosynthetic protein complexes in plant thylakoid membrane are nonhomogenously distributed between stacked and unstacked membrane regions. Yet, the sublocalization of the central cytochrome b6f complex remains controversial. We present a structural model that explains the variation in cytochrome b6f sublocalization data. Small changes in the distance between adjacent membranes in stacked grana regions either allow or restrict access of cytochrome b6f complexes to grana...
May 5, 2017: Trends in Plant Science
keyword
keyword
11413
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"