Bingkun Yang, Yahui Li, Wenyan Guo, Qingning Zhang, Liangyu Pan, Kunfeng Duan, Panpan Zhang, Leiming Ren, Wei Zhang, Qiao Wang, Dezhi Kong
Cerebrolysin (CBL) is a peptide-rich preparation made by hydrolysis and purified extraction of porcine brain. CBL contains various neuroprotective peptides, such as neurotrophic factor, nerve growth factor and ciliary neurotrophic factor, which can be used to treat neurodegenerative diseases. However, the active peptides in CBL had not been studied in depth. In this study, the following was carried out in order to investigate the active peptides in CBL. First, CBL samples were treated using organic reagents (acetonitrile and acetone) to precipitate the proteins and different solid phase extraction methods (MCX mixed-mode cartridges, C18 SPE cartridge columns and HILIC sorbent)...
May 17, 2023: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences