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PhoQ/PhoP

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https://www.readbyqxmd.com/read/28805355/sensing-mg-2-contributes-to-the-resistance-of-pseudomonas-aeruginosa-to-complement-mediated-opsonophagocytosis
#1
Mohammad Qadi, Sofía Izquierdo-Rabassa, Margalida Mateu Borrás, Antonio Doménech-Sánchez, Carlos Juan, Joanna B Goldberg, Robert E W Hancock, Sebastián Albertí
Pseudomonas aeruginosa adaptation to survive in the host hinges on its ability to probe the environment and respond appropriately. Rapid adaptation is often mediated by two-component regulatory systems, such as the PhoP/PhoQ system that responds to Mg(2+) ion concentration. However, there is limited information about the role of PhoQ in P. aeruginosa bloodstream infections. We used a murine model of systemic infection to test the virulence of a PhoQ-deficient mutant. Mutation of PhoQ impaired the virulence and the ability to cause bacteremia of P...
August 14, 2017: Environmental Microbiology
https://www.readbyqxmd.com/read/28598557/computational-studies-of-the-active-and-inactive-regulatory-domains-of-response-regulator-phop-using-molecular-dynamics-simulations
#2
Xiao-Yu Qing, Hans Steenackers, Tom Venken, Marc De Maeyer, Arnout Voet
The response regulator PhoP is part of the PhoP/PhoQ two-component system, which is responsible for regulating the expression of multiple genes involved in controlling virulence, biofilm formation, and resistance to antimicrobial peptides. Therefore, modulating the transcriptional function of the PhoP protein is a promising strategy for developing new antimicrobial agents. There is evidence suggesting that phosphorylation-mediated dimerization in the regulatory domain of PhoP is essential for its transcriptional function...
June 9, 2017: Molecular Informatics
https://www.readbyqxmd.com/read/28550013/an-essential-regulatory-system-originating-from-polygenic-transcriptional-rewiring-of-phop-phoq-of-xanthomonas-campestris
#3
Bao-Yu Peng, Yue Pan, Ru-Jiao Li, Jin-Wei Wei, Fang Liang, Li Wang, Fang-Fang Wang, Wei Qian
How essential, regulatory genes originate and evolve is intriguing because mutations of these genes not only lead to lethality in organisms, but also have pleiotropic effects since they control the expression of multiple downstream genes. Therefore, the evolution of essential, regulatory genes is not only determined by genetic variations of their own sequences, but also by the biological function of downstream genes and molecular mechanisms of regulation. To understand the origin of essential, regulatory genes, experimental dissection of the complete regulatory cascade is needed...
May 26, 2017: Genetics
https://www.readbyqxmd.com/read/28470375/subtractive-protein-profiling-of-salmonella-typhimurium-biofilm-treated-with-dmso
#4
Mohd Fakharul Zaman Raja Yahya, Zazali Alias, Saiful Anuar Karsani
Salmonella typhimurium is an important biofilm-forming bacteria. It is known to be resistant to a wide range of antimicrobials. The present study was carried out to evaluate the effects of dimethyl sulfoxide (DMSO) against S. typhimurium biofilm and investigate whole-cell protein expression by biofilm cells following treatment with DMSO. Antibiofilm activities were assessed using pellicle assay, crystal violet assay, colony-forming unit counting and extracellular polymeric substance (EPS) matrix assay whilst differential protein expression was investigated using a combination of one dimensional sodium dodecyl sulfate polyacrylamide gel electrophoresis, tandem mass spectrometry and bioinformatics...
August 2017: Protein Journal
https://www.readbyqxmd.com/read/28242665/molecular-epidemiology-of-colistin-resistant-carbapenemase-producing-klebsiella-pneumoniae-in-serbia-from-2013-to-2016
#5
Katarina Novović, Anika Trudić, Snežana Brkić, Zorica Vasiljević, Milan Kojić, Deana Medić, Ivana Ćirković, Branko Jovčić
Twenty-seven colistin-resistant, carbapenemase-producing Klebsiella pneumoniae isolates were identified from hospitals in Serbia. All isolates were blaCTX-M-15 positive; ST101, ST888, ST437, ST336, and ST307 were blaOXA-48 positive; and ST340 was blaNDM-1 positive. ST307 had an insertion, and ST336 had a premature stop codon in the mgrB gene. Amino acid substitutions were detected in PmrAB of isolates ST101, ST888, ST336, and ST307. The mcr-1 and mcr-2 were not detected. An increase in phoP, phoQ, and pmrK gene transcription was detected for all sequence types...
May 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/27987256/the-regulation-of-antimicrobial-peptide-resistance-in-the-transition-to-insect-symbiosis
#6
Adam L Clayton, Shinichiro Enomoto, Yinghua Su, Colin Dale
Many bacteria utilize two-component systems consisting of a sensor kinase and a transcriptional response regulator to detect environmental signals and modulate gene expression for adaptation. The response regulator PhoP and its cognate sensor kinase PhoQ compose a two-component system known for its role in responding to low levels of Mg(2+) , Ca(2+) , pH and to the presence of antimicrobial peptides and activating the expression of genes involved in adaptation to host association. Compared with their free-living relatives, mutualistic insect symbiotic bacteria inhabit a static environment where the requirement for sensory functions is expected to be relaxed...
March 2017: Molecular Microbiology
https://www.readbyqxmd.com/read/27618760/a-comparative-proteomic-analysis-of-salmonella-typhimurium-under-the-regulation-of-the-rsta-rstb-and-phop-phoq-systems
#7
Trung-Kien Tran, Qiang-Qiang Han, Yixin Shi, Lin Guo
In pathogenic bacteria, the two-component regulatory systems (TCSs) play important roles in signal transduction and regulation of their pathogenesis. Here, we used quantitative proteomic methods to comparatively analyze functional networks under the control of the RstA/RstB system versus the PhoP/PhoQ system in Salmonella typhimurium. By comparing the proteomic profile from a wild-type strain to that from a ΔrstB strain or a ΔphoPQ strain under a condition known to activate these TCSs, we found that the levels of 159 proteins representing 6...
December 2016: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/27604223/connecting-the-sequence-space-of-bacterial-signaling-proteins-to-phenotypes-using-coevolutionary-landscapes
#8
R R Cheng, O Nordesjö, R L Hayes, H Levine, S C Flores, J N Onuchic, F Morcos
Two-component signaling (TCS) is the primary means by which bacteria sense and respond to the environment. TCS involves two partner proteins working in tandem, which interact to perform cellular functions whereas limiting interactions with non-partners (i.e., cross-talk). We construct a Potts model for TCS that can quantitatively predict how mutating amino acid identities affect the interaction between TCS partners and non-partners. The parameters of this model are inferred directly from protein sequence data...
December 2016: Molecular Biology and Evolution
https://www.readbyqxmd.com/read/27602025/relevant-genes-linked-to-virulence-are-required-for-salmonella-typhimurium-to-survive-intracellularly-in-the-social-amoeba-dictyostelium-discoideum
#9
Sebastián Riquelme, Macarena Varas, Camila Valenzuela, Paula Velozo, Nicolás Chahin, Paulina Aguilera, Andrea Sabag, Bayron Labra, Sergio A Álvarez, Francisco P Chávez, Carlos A Santiviago
The social amoeba Dictyostelium discoideum has proven to be a useful model for studying relevant aspects of the host-pathogen interaction. In this work, D. discoideum was used as a model to study the ability of Salmonella Typhimurium to survive in amoebae and to evaluate the contribution of selected genes in this process. To do this, we performed infection assays using axenic cultures of D. discoideum co-cultured with wild-type S. Typhimurium and/or defined mutant strains. Our results confirmed that wild-type S...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27494907/cluster-dependent-colistin-hetero-resistance-in-enterobacter-cloacae-complex
#10
François Guérin, Christophe Isnard, Clara Sinel, Philippe Morand, Anne Dhalluin, Vincent Cattoir, Jean-Christophe Giard
OBJECTIVES: Aims of this study were to: (i) evaluate whether the cluster membership could have an impact on hetero-resistance phenotype to colistin in the Enterobacter cloacae complex (ECC); and (ii) determine the genetic mechanism of colistin hetero-resistance in ECC. METHODS: A collection of 124 clinical isolates belonging to 13 clusters were used to analyse the hetero-resistance phenotype (MICs were determined using the broth microdilution method, Etest and population analysis profiling)...
November 2016: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/27471053/antimicrobial-peptides-trigger-a-division-block-in-escherichia-coli-through-stimulation-of-a-signalling-system
#11
Srujana S Yadavalli, Jeffrey N Carey, Rachel S Leibman, Annie I Chen, Andrew M Stern, Manuela Roggiani, Andrew M Lippa, Mark Goulian
Antimicrobial peptides are an important component of the molecular arsenal employed by hosts against bacteria. Many bacteria in turn possess pathways that provide protection against these compounds. In Escherichia coli and related bacteria, the PhoQ/PhoP signalling system is a key regulator of this antimicrobial peptide defence. Here we show that treating E. coli with sublethal concentrations of antimicrobial peptides causes cells to filament, and that this division block is controlled by the PhoQ/PhoP system...
2016: Nature Communications
https://www.readbyqxmd.com/read/27447896/the-small-membrane-protein-mgrb-regulates-phoq-bifunctionality-to-control-phop-target-gene-expression-dynamics
#12
Michael E Salazar, Anna I Podgornaia, Michael T Laub
In Escherichia coli and other γ-proteobacteria, the PhoQ-PhoP two-component signaling system responds to low extracellular Mg(++) and cationic antimicrobial peptides. On transition to inducing conditions, the expression of PhoP-dependent genes increases rapidly, but then decays to a new, intermediate steady-state level, a phenomenon often referred to as partial adaptation. The molecular basis for this partial adaptation has been unclear. Here, using time-lapse fluorescence microscopy to examine PhoP-dependent gene expression in individual E...
November 2016: Molecular Microbiology
https://www.readbyqxmd.com/read/27404739/design-and-expression-of-peptides-with-antimicrobial-activity-against-salmonella-typhimurium
#13
Amalia Porta, Anna Maria Petrone, Silvana Morello, Ilaria Granata, Francesca Rizzo, Domenico Memoli, Alessandro Weisz, Bruno Maresca
We showed previously that insertion of Synechocystis Δ(12) -desaturase in salmonella's membrane alters membrane physical state (MPS), followed by the expression of stress genes causing inability to survive within murine macrophages (MΦ). Recently, we showed that expression of one membrane lipid domain (MLD) of Δ(12) -desaturase (ORF200) interferes with salmonella MPS, causing loss of virulence in mice and immunoprotection. Here, we postulate that an α-antimicrobial peptide (α-AMP) intercalates within membrane lipids, and depending on its amino acid sequence, it does so within specific key sensors of MLD...
February 2017: Cellular Microbiology
https://www.readbyqxmd.com/read/27303387/the-pathogenicity-of-pseudomonas-syringae-mb03-against-caenorhabditis-elegans-and-the-transcriptional-response-of-nematicidal-genes-upon-different-nutritional-conditions
#14
Muhammad Ali, Yu Sun, Li Xie, Huafu Yu, Anum Bashir, Lin Li
Different species of the Pseudomonas genus have been reported for their pathogenic potential against animal cells. However, the pathogenicity of Pseudomonas syringae against Caenorhabditis elegans has never been reported. In this study, the interaction of P. syringae MB03 with C. elegans was studied. Different bioassays such as killing assay, lawn leaving assay, food preference assay, L4 growth assay and newly developed "secretion assay" were performed to evaluate the pathogenic potential of P. syringae on different growth media...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27282333/acidic-ph-sensing-in-the-bacterial-cytoplasm-is-required-for-salmonella-virulence
#15
Jeongjoon Choi, Eduardo A Groisman
pH regulates gene expression, biochemical activities and cellular behaviors. A mildly acidic pH activates the master virulence regulatory system PhoP/PhoQ in the facultative intracellular pathogen Salmonella enterica serovar Typhimurium. The sensor PhoQ harbors an extracytoplasmic domain implicated in signal sensing, and a cytoplasmic domain controlling activation of the regulator PhoP. We now report that, surprisingly, a decrease in Salmonella's own cytoplasmic pH induces transcription of PhoP-activated genes even when the extracytoplasmic pH remains neutral...
September 2016: Molecular Microbiology
https://www.readbyqxmd.com/read/27096785/modulation-of-virulence-genes-by-the-two-component-system-phop-phoq-in-avian-pathogenic-escherichia-coli
#16
Jian Tu, Boyan Huang, Yu Zhang, Yuxi Zhang, Ting Xue, Shaocan Li, Kezong Qi
Avian pathogenic Escherichia coli (APEC) infections are a very important problem in the poultry industry. PhoP-PhoQ is a two-component system that regulates virulence genes in APEC. In this study, we constructed strains that lacked the PhoP or PhoQ genes to assess regulation of APEC pathogenicity by the PhoP-PhoQ two-component system. The PhoP mutant strain AE18, PhoQ mutant strain AE19, and PhoP/PhoQ mutant strain AE20 were constructed by the Red homologous recombination method. Swim plates were used to evaluate the motility of the APEC strains, viable bacteria counting was used to assess adhesion and invasion of chick embryo fibroblasts, and Real-Time PCR was used to measure mRNA expression of virulence genes...
2016: Polish Journal of Veterinary Sciences
https://www.readbyqxmd.com/read/27014218/crp-is-an-activator-of-yersinia-pestis-biofilm-formation-that-operates-via-a-mechanism-involving-gmha-and-waaae-coad
#17
Lei Liu, Haihong Fang, Huiying Yang, Yiquan Zhang, Yanping Han, Dongsheng Zhou, Ruifu Yang
gmhA encodes a phosphoheptose isomerase that catalyzes the biosynthesis of heptose, a conserved component of lipopolysaccharide (LPS). GmhA plays an important role in Yersinia pestis biofilm blockage in the flea gut. waaA, waaE, and coaD constitute a three-gene operon waaAE-coaD in Y. pestis. waaA encodes a transferase that is responsible for binding lipid-A to the core oligosaccharide of LPS. WaaA is a key determinant in Y. pestis biofilm formation, and the waaA expression is positively regulated by the two-component regulatory system PhoP/PhoQ...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/26943369/acetylation-of-lysine-201-inhibits-the-dna-binding-ability-of-phop-to-regulate-salmonella-virulence
#18
Jie Ren, Yu Sang, Yongcong Tan, Jing Tao, Jinjing Ni, Shuting Liu, Xia Fan, Wei Zhao, Jie Lu, Wenjuan Wu, Yu-Feng Yao
The two-component system PhoP-PhoQ is highly conserved in bacteria and regulates virulence in response to various signals for bacteria within the mammalian host. Here, we demonstrate that PhoP could be acetylated by Pat and deacetylated by deacetylase CobB enzymatically in vitro and in vivo in Salmonella Typhimurium. Specifically, the conserved lysine residue 201(K201) in winged helix-turn-helix motif at C-terminal DNA-binding domain of PhoP could be acetylated, and its acetylation level decreases dramatically when bacteria encounter low magnesium, acid stress or phagocytosis of macrophages...
March 2016: PLoS Pathogens
https://www.readbyqxmd.com/read/26780187/the-magnesium-transporter-a-is-activated-by-cardiolipin-and-is-highly-sensitive-to-free-magnesium-in-vitro
#19
Saranya Subramani, Harmonie Perdreau-Dahl, Jens Preben Morth
The magnesium transporter A (MgtA) is a specialized P-type ATPase, believed to import Mg(2+) into the cytoplasm. In Salmonella typhimurium and Escherichia coli, the virulence determining two-component system PhoQ/PhoP regulates the transcription of mgtA gene by sensing Mg(2+) concentrations in the periplasm. However, the factors that affect MgtA function are not known. This study demonstrates, for the first time, that MgtA is highly dependent on anionic phospholipids and in particular, cardiolipin. Colocalization studies confirm that MgtA is found in the cardiolipin lipid domains in the membrane...
January 18, 2016: ELife
https://www.readbyqxmd.com/read/26472331/analysis-of-salmonella-phop-phoq-regulation-by-dimethyl-srm-based-quantitative-proteomics
#20
Li-Zhi Hu, Wei-Ping Zhang, Mao-Tian Zhou, Qiang-Qiang Han, Xiao-Li Gao, Hao-Long Zeng, Lin Guo
SRM (selected reaction monitoring), a tandem mass spectrometry-based method characterized by high repeatability and accuracy, is an effective tool for the quantification of predetermined proteins. In this study, we built a time-scheduled dimethyl-SRM method that can provide the precise relative quantification of 92 proteins in one run. By applying this method to the Salmonella PhoP/PhoQ two-component system, we found that the expression of selected PhoP/PhoQ-activated proteins in response to Mg(2+) concentrations could be divided into two distinct patterns...
January 2016: Biochimica et Biophysica Acta
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