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Lean laboratory

Rachel V van Schendel, Lieve Page-Christiaens, Lean Beulen, Catia M Bilardo, Marjon A de Boer, Audrey B C Coumans, Brigitte H Faas, Irene M van Langen, Klaske D Lichtenbelt, Merel C van Maarle, Merryn V E Macville, Dick Oepkes, Eva Pajkrt, Lidewij Henneman
OBJECTIVE: To evaluate preferences and decision-making amongst high-risk pregnant women offered a choice between Non-Invasive Prenatal Testing (NIPT), invasive testing or no further testing. METHODS: Nationwide implementation study (TRIDENT) offering NIPT as contingent screening test for women at increased risk for fetal aneuploidy based on first-trimester combined testing (>1:200) or medical history. A questionnaire was completed after counseling assessing knowledge, attitudes and participation following the Multidimensional Measure of Informed Choice...
October 14, 2016: Prenatal Diagnosis
Yohei Shimokochi, Jatin P Ambegaonkar, Eric G Meyer
CONTEXT:  Ground reaction force (GRF) and tibiofemoral force magnitudes and directions have been shown to affect anterior cruciate ligament loading during landing. However, the kinematic and kinetic factors modifying these 2 forces during landing are unknown. OBJECTIVE:  To clarify the intersegmental kinematic and kinetic links underlying the alteration of the GRF and tibiofemoral force vectors secondary to changes in the sagittal-plane body position during single-legged landing...
October 10, 2016: Journal of Athletic Training
Sharon Matheny, Robert Tebbs, Erin Crowley, David Goins, Angelia Burrell, Patrick Bird, Michelle Swimley, M Joseph Benzinger, Jonathan Flannery, Jonathan Cloke, James Agin, Benjamin Bastin
The Applied Biosystems™ RapidFinder™ STEC Detection Workflow (Thermo Fisher Scientific) is a complete protocol for the rapid qualitative detection of Escherichia coli (E. coli) O157:H7 and the "Big 6" non-O157 Shiga-like toxin-producing E. coli (STEC) serotypes (defined as serogroups: O26, O45, O103, O111, O121, and O145). The RapidFinder STEC Detection Workflow makes use of either the automated preparation of PCR-ready DNA using the Applied Biosystems PrepSEQ™ Nucleic Acid Extraction Kit in conjunction with the Applied Biosystems MagMAX™ Express 96-well magnetic particle processor or the Applied Biosystems PrepSEQ Rapid Spin kit for manual preparation of PCR-ready DNA...
October 1, 2016: Journal of AOAC International
J A Feldpausch, R G Amachawadi, M D Tokach, H M Scott, T G Nagaraja, S S Dritz, R D Goodband, J C Woodworth, J M DeRouchey
A total of 480 pigs (PIC 327 × 1050; initially 48.7 ± 2.3 kg) were used to determine the interactive effects of supplemental Cu, Zn, and ractopamine HCl (RAC) on finishing pig growth performance, carcass characteristics, and antimicrobial susceptibility of enteric bacteria. Treatments were arranged in a 2 × 2 × 2 factorial with the main effects of added Cu (CuSO; 0 vs. 125 mg/kg Cu), Zn (ZnO; 0 vs. 150 mg/kg Zn), and RAC (0 vs. 10 mg/kg during the last 28 d prior to marketing). All diets contained 11 mg/kg Cu and 73 mg/kg Zn from the trace mineral premix...
August 2016: Journal of Animal Science
Feng Yang, George A King
Treadmill has been broadly used in laboratory and rehabilitation settings for the purpose of facilitating human locomotion analysis and gait training. The objective of this study was to determine whether dynamic gait stability differs or resembles between the two walking conditions (overground vs. treadmill) among young adults. Fifty-four healthy young adults (age: 23.9±4.7years) participated in this study. Each participant completed five trials of overground walking followed by five trials of treadmill walking at a self-selected speed while their full body kinematics were gathered by a motion capture system...
September 20, 2016: Journal of Electromyography and Kinesiology
Kyung Hee Lee, Jang-Hee Cho, Owen Kwon, Sang-Un Kim, Ryang Hi Kim, Young Wook Cho, Hee-Yeon Jung, Ji-Young Choi, Chan-Duck Kim, Yong-Lim Kim, Sun-Hee Park
BACKGROUND: Prealbumin, a sensitive marker for protein-energy status, is also known as an independent risk factor for mortality in hemodialysis patients. We investigated the impact of prealbumin on survival in incident peritoneal dialysis (PD) patients. METHODS: In total, 136 incident PD patients (mean age, 53.0 ± 15.8 years) between 2002 and 2007 were enrolled in the study. Laboratory data, dialysis adequacy, and nutritional parameters were assessed 3 months after PD initiation...
September 2016: Kidney Research and Clinical Practice
André Brännström, Ji-Guo Yu, Per Jonsson, Torbjörn Åkerfeldt, Mats Stridsberg, Michael Svensson
The present work investigated serum vitamin D (25(OH)D) status in relation to bone and muscle qualities and functions in 19 female soccer players (13-16 years) resident at northern latitude with very low sun exposure (∼32-36 h/month) during winter season (late January to early March). Serum 25(OH)D, parathyroid hormone and bone turnover markers osteocalcin (OC) and beta carboxy-terminal collagen cross-links (β-Ctx), as well as body composition and muscle performance were examined. Hormones were tested using routine laboratory methods...
September 15, 2016: European Journal of Sport Science
Radamés Maciel Vítor Medeiros, Eduardo da Silva Alves, Valdir de Aquino Lemos, Paulo Adriano Schwingel, Andressa da Silva, Roberto Vital, Alexandre Silva Vieira, Murilo Moreira Barreto, Edilson Alves da Rocha, Sergio Tufik, Marco Túlio de Mello
CONTEXT: Body composition assessments of high-performance athletes are very important for identifying physical performance potential. Although the relationship between the kinanthropometric characteristics and performance abilities of Olympic swimmers is extremely important, this subject is not completely understood for Paralympic swimmers. OBJECTIVE: To investigate the relationship between body composition and sport performance in Brazilian Paralympic swimmers 6 months after training...
August 24, 2016: Journal of Sport Rehabilitation
Stephen M Ford, Liz Simon, Curtis Vande Stouwe, Tim Allerton, Donald E Mercante, Lauri O Byerley, Jason P Dufour, Gregory J Bagby, Steve Nelson, Patricia E Molina
Alcohol use disorders (AUDs) frequently exist among persons living with HIV/AIDS (PLWHA). Chronic alcohol consumption, HIV infection, and anti-retroviral therapy (ART) are independently associated with impairments in glucose-insulin dynamics. Previous studies from our laboratory have shown that chronic binge alcohol (CBA) administration decreases body mass index, attenuates weight gain, and accentuates skeletal muscle wasting at end-stage disease in non-ART treated simian immunodeficiency virus (SIV)-infected male rhesus macaques...
September 7, 2016: American Journal of Physiology. Regulatory, Integrative and Comparative Physiology
Roger Woolf, Amanda Locke, Catherine Potts
PURPOSE: Pharmacist prescribing as part of a collaborative drug therapy agreement (CDTA) within an integrated health system in Washington is described. SUMMARY: Virginia Mason Medical Center (VMMC) in Seattle, Washington, uses a team-based care model with broad-based CDTAs to provide quality patient care. The majority of patients are referred to the pharmacist after a diagnosis has been made and a clinical care plan has been started. The pharmacist manages the patient's care within his or her scope of practice as defined by state laws and further detailed by VMMC internal protocols...
September 15, 2016: American Journal of Health-system Pharmacy: AJHP
Liang Shi, Chaoming Mao, Xuefeng Wang, Rencong Liu, Lin Li, Xiao Mou, Ping Xu, Hongli Li, Chengcheng Xu, Guoyue Yuan, Bin Wang, Hao Zhang
Increasing evidence suggests the association between obesity and bone metabolism. However, whether excessive fat accumulation has a beneficial or adverse effect on bone health remains controversial. Chemerin is a novel adipocyte-derived hormone and a chemoattractant cytokine that regulates adipogenesis. This study was performed to investigate the associations of serum chemerin with bone mineral density (BMD) and serum pro-inflammatory cytokine levels in 543 Chinese obese postmenopausal women. BMD of the femoral neck and lumbar spine, lean mass, and fat mass were measured using dual energy X-ray absorptiometry...
August 2016: Medicine (Baltimore)
Abdurrahman Coskun, Mustafa Serteser, Resul Altinayak, Ibrahim Unsal
No abstract text is available yet for this article.
August 31, 2016: Clinical Chemistry and Laboratory Medicine: CCLM
Frederick Goetz, Sara E Smith, Giles Goetz, Cheryl A Murphy
BACKGROUND: The sea lamprey (Petromyzon marinus) is a jawless vertebrate that parasitizes fish as an adult and, with overfishing, was responsible for the decline in lake trout (Salvelinus namaycush) populations in the Great Lakes. While laboratory studies have looked at the rates of wounding on various fish hosts, there have been few investigations on the physiological effects of lamprey wounding on the host. In the current study, two morphotypes of lake trout, leans and siscowets, were parasitized in the laboratory by sea lampreys and the liver transcriptomes of parasitized and nonparasitized fish were analyzed by RNA-seq (DESeq2 and edgeR) to determine which genes and gene pathways (Ingenuity Pathway Analysis) were altered by lamprey parasitism...
2016: BMC Genomics
Keith D Rein, Sukesh Roy, Scott T Sanders, Andrew W Caswell, Frederick R Schauer, James R Gord
A mid-infrared fiber-coupled laser system constructed around three time-division-multiplexed quantum-cascade lasers capable of measuring the absorption spectra of CO, CO<sub>2</sub>, and N<sub>2</sub>O at 100 kHz over a wide range of operating pressures and temperatures is demonstrated. This system is first demonstrated in a laboratory burner and then used to measure temperature, pressure, and concentrations of CO, CO<sub>2</sub>, and N<sub>2</sub>O as a function of time in a detonated mixture of N<sub>2</sub>O and C<sub>3</sub>H<sub>8</sub>...
August 10, 2016: Applied Optics
Alexandra Feldman, Sebastian K Eder, Thomas K Felder, Lyudmyla Kedenko, Bernhard Paulweber, Andreas Stadlmayr, Ursula Huber-Schönauer, David Niederseer, Felix Stickel, Simon Auer, Elisabeth Haschke-Becher, Wolfgang Patsch, Christian Datz, Elmar Aigner
OBJECTIVES: Non-alcoholic fatty liver disease (NAFLD) is closely linked to obesity; however, 5-8% of lean subjects also have evidence of NAFLD. We aimed to investigate clinical, genetic, metabolic and lifestyle characteristics in lean Caucasian subjects with NAFLD. METHODS: Data from 187 subjects allocated to one of the three groups according to body mass index (BMI) and hepatic steatosis on ultrasound were obtained: lean healthy (BMI≤25 kg/m(2), no steatosis, N=71), lean NAFLD (BMI≤25 kg/m(2), steatosis, N=55), obese NAFLD (BMI≥30 kg/m(2), steatosis; N=61)...
August 16, 2016: American Journal of Gastroenterology
Lean Beulen, Brigitte H W Faas, Ilse Feenstra, John M G van Vugt, Mireille N Bekker
OBJECTIVE: This study aims to evaluate the application of non-invasive prenatal testing (NIPT) as an alternative to invasive diagnostic prenatal testing for pregnancies with abnormal ultrasound findings. METHOD: A retrospective analysis was performed of 251 single and multiple pregnancies at high risk for fetal chromosomal abnormalities based on ultrasonographic examination, where NIPT was performed as first-tier genetic test. NIPT was performed by massively parallel sequencing of cell-free DNA in maternal plasma, allowing genome-wide detection of whole-chromosome as well as partial autosomal aneuploidies, as currently in the Dutch laboratories, sex chromosomes are not analysed...
August 12, 2016: Ultrasound in Obstetrics & Gynecology
Rafael Machado Mantovani, Natália Pessoa Rocha, Daniel Massote Magalhães, Izabela Guimarães Barbosa, Antônio Lúcio Teixeira, Ana Cristina Simões E Silva
OBJECTIVE: Childhood obesity has been associated with metabolic syndrome and cardiovascular diseases. This study aimed to compare plasma levels of traditional metabolic markers, adipokines and soluble tumor necrosis factor receptor type 1 (sTNFR1) in overweight, obese and lean children. We also assessed the relationships of these molecules with classical metabolic risk factors. METHODS: This study included 104 children and adolescents, which were grouped as: lean (n=24), overweight (n=30), and obese subjects (n=50)...
August 6, 2016: Jornal de Pediatria
Shikha S Sundaram, Ann Halbower, Zhaoxing Pan, Kristen Robbins, Kelley E Capocelli, Jelena Klawitter, Colin T Shearn, Ronald J Sokol
BACKGROUND & AIMS: Oxidative stress is proposed as a central mediator in NAFLD pathogenesis, but the specific trigger for reactive oxygen species generation has not been clearly delineated. In addition, emerging evidence shows that obesity related obstructive sleep apnea (OSA) and nocturnal hypoxia are associated with NAFLD progression in adults. The aim of this study was to determine if OSA/nocturnal hypoxia-induced oxidative stress promotes the progression of pediatric NAFLD. METHODS: Subjects with biopsy proven NAFLD and lean controls were studied...
September 2016: Journal of Hepatology
Deanna M Arble, Joshua W Pressler, Joyce Sorrell, Rachel Wevrick, Darleen A Sandoval
BACKGROUND: Prader-Willi syndrome (PWS) is a genetic disorder characterized by hyperphagia, obesity, cardiopulmonary diseases, and increased mortality. Although successful weight loss improves health in PWS, few treatments cause sustained weight loss in obese patients let alone obese individuals with PWS. OBJECTIVES: The present study uses the Magel2 knockout (KO) mouse, an animal model of PWS, to conduct a preclinical study on the efficacy of sleeve gastrectomy (SG) in PWS...
April 27, 2016: Surgery for Obesity and related Diseases: Official Journal of the American Society for Bariatric Surgery
S Mariani, D Costantini, C Lubrano, S Basciani, C Caldaroni, G Barbaro, E Poggiogalle, L M Donini, A Lenzi, L Gnessi
BACKGROUND AND AIM: Obesity is increasing worldwide and is related to undesirable cardiovascular outcomes. Epicardial fat (EF), the heart visceral fat depot, increases with obesity and correlates with cardiovascular risk. SIRT1, an enzyme regulating metabolic circuits linked with obesity, has a cardioprotective effect and is a predictor of cardiovascular events. We aimed to assess the relationship of EF thickness (EFT) with circulating SIRT1 in patients with obesity. METHODS AND RESULTS: Sixty-two patients affected by obesity and 23 lean controls were studied...
June 16, 2016: Nutrition, Metabolism, and Cardiovascular Diseases: NMCD
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