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https://www.readbyqxmd.com/read/28335451/a-review-of-phage-therapy-against-bacterial-pathogens-of-aquatic-and-terrestrial-organisms
#1
REVIEW
Janis Doss, Kayla Culbertson, Delilah Hahn, Joanna Camacho, Nazir Barekzi
Since the discovery of bacteriophage in the early 1900s, there have been numerous attempts to exploit their innate ability to kill bacteria. The purpose of this report is to review current findings and new developments in phage therapy with an emphasis on bacterial diseases of marine organisms, humans, and plants. The body of evidence includes data from studies investigating bacteriophage in marine and land environments as modern antimicrobial agents against harmful bacteria. The goal of this paper is to present an overview of the topic of phage therapy, the use of phage-derived protein therapy, and the hosts that bacteriophage are currently being used against, with an emphasis on the uses of bacteriophage against marine, human, animal and plant pathogens...
March 18, 2017: Viruses
https://www.readbyqxmd.com/read/28329379/the-lysis-of-pathogenic-escherichia-coli-by-bacteriophages-releases-less-endotoxin-than-betalactams
#2
Nicolas Dufour, Raphaëlle Delattre, Jean-Damien Ricard, Laurent Debarbieux
Background: Beside numerous experimental data assessing phage therapy efficacy, questions regarding safety of this approach are not sufficiently addressed. In particular, as phages can kill bacterial cells within less than 10 minutes, the associated endotoxin release (ER) in severe infections caused by Gram-negative bacteria could be a matter of concern. Methods: Two therapeutic virulent phages and four reference antibiotics were studied in vitro for their ability to kill two pathogenic strains of Escherichia coli and generate an ER...
March 1, 2017: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America
https://www.readbyqxmd.com/read/28329182/phage-therapy-considerations-and-challenges-for-development
#3
Rebecca Reindel, Cara R Fiore
No abstract text is available yet for this article.
March 1, 2017: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America
https://www.readbyqxmd.com/read/28302013/multicyclic-peptides-as-scaffolds-for-the-development-of-tumor-targeting-agents
#4
Anastasia Loktev, Uwe Haberkorn, Walter Mier
The lack of specificity of traditional cytotoxic drugs triggers the development of anticancer agents with high selectivity to tumor-specific proteins. The unveiling of target structures such as EGFR or Her2 allows the focused development of novel therapies and has strongly advanced tumor treatment. Tumor-specific high-affinity ligands can be identified by using display techniques such as phage, yeast surface, ribosome and mRNA display. These techniques enable the screening of huge libraries, consequently providing a valuable alternative to rational drug development...
March 16, 2017: Current Medicinal Chemistry
https://www.readbyqxmd.com/read/28299517/characterization-and-complete-genome-sequence-analysis-of-a-novel-virulent-siphoviridae-phage-against-staphylococcus-aureus-isolated-from-bovine-mastitis-in-xinjiang-china
#5
Qian Zhang, Shaozhen Xing, Qiang Sun, Guangqian Pei, Shi Cheng, Yannan Liu, Xiaoping An, Xianglilan Zhang, Yonggang Qu, Yigang Tong
Bovine mastitis is one of the most costly diseases in dairy cows worldwide. It can be caused by over 150 different microorganisms, where Staphylococcus aureus is the most frequently isolated and a major pathogen responsible for heavy economic losses in dairy industry. Although antibiotic therapy is most widely used, alternative treatments are necessary due to the increasing antibiotic resistance. Using phage for pathogen control is a promising tool in the fight against antibiotic resistance. Mainly using high-throughput sequencing, bioinformatics and our proposed phage termini identification method, we have isolated and characterized a novel virulent phage, designated as vB_SauS_IMEP5, from manure collected from dairy farms in Shihezi, Xinjiang, China, for use as a biocontrol agent against Staphylococcus aureus infections...
March 15, 2017: Virus Genes
https://www.readbyqxmd.com/read/28291834/the-isolation-and-characterization-of-stenotrophomonas-maltophilia-t4-like-bacteriophage-dlp6
#6
Danielle L Peters, Paul Stothard, Jonathan J Dennis
Increasing isolation of the extremely antibiotic resistant bacterium Stenotrophomonas maltophilia has caused alarm worldwide due to the limited treatment options available. A potential treatment option for fighting this bacterium is 'phage therapy', the clinical application of bacteriophages to selectively kill bacteria. Bacteriophage DLP6 (vB_SmoM-DLP6) was isolated from a soil sample using clinical isolate S. maltophilia strain D1571 as host. Host range analysis of phage DLP6 against 27 clinical S. maltophilia isolates shows successful infection and lysis in 13 of the 27 isolates tested...
2017: PloS One
https://www.readbyqxmd.com/read/28289407/a-novel-antimicrobial-endolysin-lyspa26-against-pseudomonas-aeruginosa
#7
Mingquan Guo, Chunyan Feng, Jie Ren, Xuran Zhuang, Yan Zhang, Yongzhang Zhu, Ke Dong, Ping He, Xiaokui Guo, Jinhong Qin
The global increase in multidrug resistant (MDR) bacteria has led to phage therapy being refocused upon. A novel endolysin, LysPA26, containing a lysozyme-like domain, was screened against Pseudomonas aeruginosa in this study. It had activity against MDR P. aeruginosa without pretreatment with an outer-membrane permeabilizer. LysPA26 could kill up to 4 log units P. aeruginosa in 30 min. In addition, temperature and pH effect assays revealed that LysPA26 had good stability over a broad range of pH and temperatures...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28286740/long-term-safety-of-topical-bacteriophage-application-to-the-frontal-sinus-region
#8
Amanda J Drilling, Mian L Ooi, Dijana Miljkovic, Craig James, Peter Speck, Sarah Vreugde, Jason Clark, Peter-John Wormald
Background:Staphylococcus aureus biofilms contribute negatively to a number of chronic conditions, including chronic rhinosinusitis (CRS). With the inherent tolerance of biofilm-bound bacteria to antibiotics and the global problem of bacterial antibiotic resistance, the need to develop novel therapeutics is paramount. Phage therapy has previously shown promise in treating sinonasal S. aureus biofilms. Methods: This study investigates the long term (20 days) safety of topical sinonasal flushes with bacteriophage suspensions...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28259978/ectopic-expression-of-the-atp-synthase-%C3%AE-subunit-on-the-membrane-of-pc-3m-cells-supports-its-potential-role-in-prostate-cancer-metastasis
#9
Wei Li, Yulin Li, Gaiyun Li, Zilong Zhou, Xiaona Chang, Yang Xia, Xinjie Dong, Zhijing Liu, Bo Ren, Wei Liu, Yilei Li
Metastatic prostate cancer is associated with high mortality rates. Identification of metastasis-related proteins may facilitate the development of novel therapies for the treatment of metastatic disease. In the present study, we aimed to identify prostate cancer metastasis-associated membrane proteins. We developed a phage-displayed 7-mer peptide library to screen the target peptides that were specifically bound to PC-3M cells with subtractive panning from normal prostate cells and PC-3 prostate cancer cells...
February 16, 2017: International Journal of Oncology
https://www.readbyqxmd.com/read/28258668/pathogenic-factors-of-pseudomonas-aeruginosa-the-role-of-biofilm-in-pathogenicity-and-as-a-target-for-phage-therapy
#10
Fairoz Al-Wrafy, Ewa Brzozowska, Sabina Górska, Andrzej Gamian
Pseudomonas aeruginosa is an opportunistic pathogen that can cause several acute and chronic infections in humans, and it has become an important cause of nosocomial infections and antibiotic resistance. Biofilm represents an important virulence factor for these bacteria, plays a role in P. aeruginosa infections and avoidance of immune defence mechanisms, and has the ability to protect the bacteria from antibiotics. Alginate, Psl and Pel, three exopolysaccharides, are the main components in biofilm matrix, with many biological functions attributed to them, especially with respect to the protection of the bacterial cell from antibiotics and the immune system...
February 14, 2017: Postȩpy Higieny i Medycyny Doświadczalnej
https://www.readbyqxmd.com/read/28230780/a3r-phage-and-staphylococcus-aureus-lysate-do-not-induce-neutrophil-degranulation
#11
Jan Borysowski, Ryszard Międzybrodzki, Piotr Wierzbicki, Danuta Kłosowska, Grażyna Korczak-Kowalska, Beata Weber-Dąbrowska, Andrzej Górski
The objective of this study was to evaluate the effects of A3R phage and Staphylococcus aureus lysate obtained after phage infection on neutrophil degranulation. The exocytosis of primary and secondary granules from neutrophils was investigated in vitro in whole blood specimens by flow cytometry based on the expression of specific markers of exocytosis (CD63 for primary granules and CD66b for secondary granules). We found that both A3R and S. aureus lysate had no significant effect on the exocytosis of primary and secondary granules...
February 21, 2017: Viruses
https://www.readbyqxmd.com/read/28213104/isolation-and-characterization-of-a-virulent-bacteriophage-infecting-acinetobacter-johnsonii-from-activated-sludge
#12
Niansi Fan, Rong Qi, Min Yang
A double-stranded DNA phage named AJO1, infecting Acinetobacter johnsonii, which plays an important role in wastewater treatment, was isolated from activated sludge in a full-scale municipal wastewater treatment plant. Based on morphological taxonomy, AJO1, with an icosahedral head 55 ± 2 nm in diameter and a non-contractile tail 8 ± 2 nm in length, was classified as a member of the Podoviridae family. Bacterial infection characteristics were as follows: no polyvalent infectivity, optimal multiplicity of infection of 10(-2); eclipse and burst size of 30 min and 51...
February 14, 2017: Research in Microbiology
https://www.readbyqxmd.com/read/28186116/targeting-of-phage-particles-towards-endothelial-cells-by-antibodies-selected-through-a-multi-parameter-selection-strategy
#13
Ole A Mandrup, Simon Lykkemark, Peter Kristensen
One of the hallmarks of cancer is sustained angiogenesis. Here, normal endothelial cells are activated, and their formation of new blood vessels leads to continued tumour growth. An improved patient condition is often observed when angiogenesis is prevented or normalized through targeting of these genomically stable endothelial cells. However, intracellular targets constitute a challenge in therapy, as the agents modulating these targets have to be delivered and internalized specifically to the endothelial cells...
February 10, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28179010/characterization-and-complete-genome-of-the-virulent-myoviridae-phage-jd007-active-against-a-variety-of-staphylococcus-aureus-isolates-from-different-hospitals-in-shanghai-china
#14
Zelin Cui, Tingting Feng, Feifei Gu, Qingtian Li, Ke Dong, Yan Zhang, Yongzhang Zhu, Lizhong Han, Jinhong Qin, Xiaokui Guo
BACKGROUND: The implementation of phage therapy is re-emerging with the increase in widespread antibiotic-resistant bacteria. METHODS: Staphylococcus phage JD007 was characterized and its complete genome sequence analysed. RESULTS: Staphylococcus phage JD007 was classified as belonging to the Myoviridae family based on its morphology, as observed by transmission electron microscopy. Its lytic activity was stable between pH 5-11 and below 42 °C; moreover, an absorbance curve showed that nearly 90% of the viral particles had adsorbed to its host after a 20 min co-incubation...
February 8, 2017: Virology Journal
https://www.readbyqxmd.com/read/28166465/isolation-and-characterization-of-large-marine-bacteriophage-myoviridae-vhkm4-infecting-vibrio-harveyi
#15
Tamrin M Lal, Motohiko Sano, Julian Ransangan
The causative agent responsible for vibriosis in tropical fish aquaculture, Vibrio harveyi, has become a major bacterial pathogen. Studies suggest that this bacterium has developed resistance to antibiotics commonly used in aquaculture. In view of this situation and the requirement for the proposed postantibiotic era, bacteriophage therapy seems to be a promising control strategy for fish vibriosis. In this study, a lytic Vibrio phage VhKM4 belonging to a member of large, marine Myoviridae was successfully isolated...
March 2017: Journal of Aquatic Animal Health
https://www.readbyqxmd.com/read/28153726/k-ras-g12d-selective-inhibitory-peptides-generated-by-random-peptide-t7-phage-display-technology
#16
Kotaro Sakamoto, Yusuke Kamada, Tomoya Sameshima, Masahiro Yaguchi, Ayumu Niida, Shigekazu Sasaki, Masanori Miwa, Shoichi Ohkubo, Jun-Ichi Sakamoto, Masahiro Kamaura, Nobuo Cho, Akiyoshi Tani
Amino-acid mutations of Gly(12) (e.g. G12D, G12V, G12C) of V-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog (K-Ras), the most promising drug target in cancer therapy, are major growth drivers in various cancers. Although over 30 years have passed since the discovery of these mutations in most cancer patients, effective mutated K-Ras inhibitors have not been marketed. Here, we report novel and selective inhibitory peptides to K-Ras(G12D). We screened random peptide libraries displayed on T7 phage against purified recombinant K-Ras(G12D), with thorough subtraction of phages bound to wild-type K-Ras, and obtained KRpep-2 (Ac-RRCPLYISYDPVCRR-NH2) as a consensus sequence...
March 11, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28146150/a-cocktail-of-three-virulent-bacteriophages-prevents-vibrio-cholerae-infection-in-animal-models
#17
Minmin Yen, Lynne S Cairns, Andrew Camilli
Effective prevention strategies will be essential in reducing disease burden due to bacterial infections. Here we harness the specificity and rapid-acting properties of bacteriophages as a potential prophylaxis therapy for cholera, a severely dehydrating disease caused by Vibrio cholerae. To this end, we test a cocktail of three virulent phages in two animal models of cholera pathogenesis (infant mouse and rabbit models). Oral administration of the phages up to 24 h before V. cholerae challenge reduces colonization of the intestinal tract and prevents cholera-like diarrhea...
February 1, 2017: Nature Communications
https://www.readbyqxmd.com/read/28139886/genomic-integration-of-the-full-length-dystrophin-coding-sequence-in-duchenne-muscular-dystrophy-induced-pluripotent-stem-cells
#18
Alfonso P Farruggio, Mital S Bhakta, Haley du Bois, Julia Ma, Michele P Calos
We developed plasmid vectors that express the full-length human dystrophin coding sequence in human cells. Dystrophin, the protein mutated in Duchenne muscular dystrophy, is extraordinarily large, providing challenges for cloning and plasmid production in E. coli. We expressed dystrophin from the strong, widely expressed CAG promoter, along with co-transcribed luciferase and mCherry marker genes useful for tracking plasmid expression. Introns were added at the 3' and 5' ends of the dystrophin sequence to prevent translation in E...
January 31, 2017: Biotechnology Journal
https://www.readbyqxmd.com/read/28128766/isolation-and-characterization-of-anti-ror1-single-chain-fragment-variable-antibodies-using-phage-display-technique
#19
Leili Aghebati-Maleki, Vahid Younesi, Farhad Jadidi-Niaragh, Behzad Baradaran, Jafar Majidi, Mehdi Yousefi
Receptor tyrosine kinase-like orphan receptor (ROR1) belongs to one of the families of receptor tyrosine kinases (RTKs). RTKs are involved in the various physiologic cellular functions including proliferation, migration, survival, signaling and differentiation. Several RTKs are deregulated in various cancers implying the targeting potential of these molecules in cancer therapy. ROR1 has recently been shown to be expressed in various types of cancer cells but not in normal adult cells. Hence a molecular inhibitor of extracellular domain of ROR1 that inhibits ROR1-cell surface interaction is of great therapeutic importance...
2017: Human Antibodies
https://www.readbyqxmd.com/read/28127392/bacterial-competition-and-quorum-sensing-signalling-shape-the-eco-evolutionary-outcomes-of-model-in-vitro-phage-therapy
#20
Rachel Mumford, Ville-Petri Friman
The rapid rise of antibiotic resistance has renewed interest in phage therapy - the use of bacteria-specific viruses (phages) to treat bacterial infections. Even though phages are often pathogen-specific, little is known about the efficiency and eco-evolutionary outcomes of phage therapy in polymicrobial infections. We studied this experimentally by exposing both quorum-sensing (QS) signalling PAO1 and QS-deficient lasR Pseudomonas aeruginosa genotypes (differing in their ability to signal intraspecifically) to lytic PT7 phage in the presence and absence of two bacterial competitors: Staphylococcus aureus and Stenotrophomonas maltophilia-two bacteria commonly associated with P...
February 2017: Evolutionary Applications
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