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https://www.readbyqxmd.com/read/29351281/automatic-labeling-of-molecular-biomarkers-of-immunohistochemistry-images-using-fully-convolutional-networks
#1
Fahime Sheikhzadeh, Rabab K Ward, Dirk van Niekerk, Martial Guillaud
This paper addresses the problem of quantifying biomarkers in multi-stained tissues based on the color and spatial information of microscopy images of the tissue. A deep learning-based method that can automatically localize and quantify the regions expressing biomarker(s) in any selected area on a whole slide image is proposed. The deep learning network, which we refer to as Whole Image (WI)-Net, is a fully convolutional network whose input is the true RGB color image of a tissue and output is a map showing the locations of each biomarker...
2018: PloS One
https://www.readbyqxmd.com/read/29351120/grading-using-ki-67-index-and-mitotic-rate-increases-the-prognostic-accuracy-of-pancreatic-neuroendocrine-tumors
#2
Prejesh Philips, David A Kooby, Shishir Maithel, Nipun B Merchant, Sharon M Weber, Emily R Winslow, Syed Ahmad, Hong J Kim, Charles R Scoggins, Kelly M McMasters, Robert C G Martin
OBJECTIVES: To measure the usefulness of Ki-67 proliferative index (Ki-67 index) as a prognostic variable for grading pancreatic neuroendocrine tumors. METHODS: A multi-institutional prospective database comprising 350 patients. Grading based on mitotic activity (<2 mitoses/10 high-power fields, 2-20 and >20) and Ki-67 index (<3% per 10 high-power fields, 3%-20% and >20%). Final grade selected based on higher grade of either variable. RESULTS: Most patients were in the less than 3% (n = 158) and 3% to 20% Ki-67 category (n = 107), with a minority being high-grade (Ki-67 > 20%, n = 27)...
January 18, 2018: Pancreas
https://www.readbyqxmd.com/read/29348852/reduced-fbxw7-expression-in-pancreatic-cancer-correlates-with-poor-prognosis-and-chemotherapeutic-resistance-via-accumulation-of-mcl1
#3
Norihiro Ishii, Kenichiro Araki, Takehiko Yokobori, Dorgormaa Gantumur, Takahiro Yamanaka, Bolag Altan, Mariko Tsukagoshi, Takamichi Igarashi, Akira Watanabe, Norio Kubo, Yasuo Hosouchi, Hiroyuki Kuwano, Ken Shirabe
Pancreatic cancer is a highly malignant tumor type with poor outcomes, and elucidation of the mechanisms involved in cancer progression and therapeutic resistance is critical. FBXW7 is a key regulator of tumor malignant potential, and its substrate MCL1 regulates therapeutic resistance in human malignancies. Therefore, determination of the relevance of FBXW7 expression is critical for improving patient outcomes. In this study, we investigated the function and clinical significance of FBXW7 in pancreatic cancer...
December 22, 2017: Oncotarget
https://www.readbyqxmd.com/read/29348677/the-proliferation-capacity-of-cultured-neural-stem-cells-promoted-by-csf-collected-from-sah-patients-correlates-to-clinical-outcome
#4
Yun-An Chen, Kuo-Chuan Wang, Der-Zen Liu, Tai-Horng Young, Li-Kai Tsai
Neurogenesis from endogenous neural stem cells (NSCs) might contribute to functional recovery after stroke based on animal studies; however, the relationship between neurogenesis and post-stroke outcome has rarely been demonstrated in humans. We prospectively collected cerebrospinal fluid (CSF) from 36 patients with subarachnoid hemorrhage (SAH). The CSF was added to the culture medium of the rat NSCs to test the effects on proliferation (proliferation index [PI], percentage of Ki-67 immunoreactive cells). We correlated the PI with functional outcome based on the modified Rankin Scale at 3 months post-SAH...
January 18, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29348487/h1-phgfk1-nanoparticles-exert-anti-tumoural-and-radiosensitising-effects-by-inhibition-of-met-in-glioblastoma
#5
Wenyan Zhang, Rui Duan, Jian Zhang, William K C Cheung, Xiaoge Gao, Raymond Zhang, Qing Zhang, Mengxue Wei, Gang Wang, Qian Zhang, Peng-Jin Mei, Hong-Lin Chen, Hsiangfu Kung, Marie C Lin, Zan Shen, Junnian Zheng, Longzhen Zhang, Hong Yao
BACKGROUND: The therapeutic resistance to ionising radiation (IR) and anti-angiogenesis mainly impair the prognosis of patients with glioblastoma. The primary and secondary MET aberrant activation is one crucial factor for these resistances. The kringle 1 domain of hepatocyte growth factor (HGFK1), an angiogenic inhibitor, contains a high-affinity binding domain of MET; however, its effects on glioblastoma remain elusive. METHODS: We formed the nanoparticles consisting of a folate receptor-targeted nanoparticle-mediated HGFK1 gene (H1/pHGFK1) and studied its anti-tumoural and radiosensitive activities in both subcutaneous and orthotopic human glioma cell-xenografted mouse models...
January 18, 2018: British Journal of Cancer
https://www.readbyqxmd.com/read/29347968/18f-fdg-pet-ct-and-diffusion-weighted-mri-for-monitoring-a-braf-and-cdk-4-6-inhibitor-combination-therapy-in-a-murine-model-of-human-melanoma
#6
Ralf S Eschbach, Philipp M Kazmierczak, Maurice M Heimer, Andrei Todica, Heidrun Hirner-Eppeneder, Moritz J Schneider, Georg Keinrath, Olga Solyanik, Jessica Olivier, Wolfgang G Kunz, Maximilian F Reiser, Peter Bartenstein, Jens Ricke, Clemens C Cyran
BACKGROUND: The purpose of the study was to investigate a novel BRAF and CDK 4/6 inhibitor combination therapy in a murine model of BRAF-V600-mutant human melanoma monitored by 18F-FDG-PET/CT and diffusion-weighted MRI (DW-MRI). METHODS: Human BRAF-V600-mutant melanoma (A375) xenograft-bearing balb/c nude mice (n = 21) were imaged by 18F-FDG-PET/CT and DW-MRI before (day 0) and after (day 7) a 1-week BRAF and CDK 4/6 inhibitor combination therapy (n = 12; dabrafenib, 20 mg/kg/d; ribociclib, 100 mg/kg/d) or placebo (n = 9)...
January 18, 2018: Cancer Imaging: the Official Publication of the International Cancer Imaging Society
https://www.readbyqxmd.com/read/29346528/a-novel-stromal-lncrna-signature-reprograms-fibroblasts-to-promote-the-growth-of-oral-squamous-cell-carcinoma-via-lncrna-caf-interleukin-33
#7
Liang Ding, Jing Ren, Dongya Zhang, Yi Li, Xiaofeng Huang, Qingang Hu, Hui Wang, Yuxian Song, Yanhong Ni, Yayi Hou
Stromal carcinoma-related fibroblasts (CAFs) are the main type of non-immune cells in the tumor microenvironment (TME). CAFs interact with cancer cells to promote tumor proliferation. Long non-coding RNAs (lncRNAs) are known to regulate cell growth, apoptosis, and metastasis of cancer cells, but their role in stromal cells is unclear. Using RNA sequencing, we identified a stromal lncRNA signature during the transformation of CAFs from normal fibroblasts (NFs) in oral squamous cell carcinoma (OSCC). We uncovered an uncharacterized lncRNA, FLJ22447, which was remarkably up-regulated in CAFs, referred to LncRNA-CAF (Lnc-CAF) hereafter...
January 13, 2018: Carcinogenesis
https://www.readbyqxmd.com/read/29346426/proliferation-and-survival-of-human-amniotic-epithelial-cells-during-their-hepatic-differentiation
#8
Julieta L Maymó, Rodrigo Riedel, Antonio Pérez-Pérez, Marta Magatti, Bernardo Maskin, José Luis Dueñas, Ornella Parolini, Víctor Sánchez-Margalet, Cecilia L Varone
Stem cells derived from placental tissues are an attractive source of cells for regenerative medicine. Amniotic epithelial cells isolated from human amnion (hAECs) have desirable and competitive characteristics that make them stand out between other stem cells. They have the ability to differentiate toward all three germ layers, they are not tumorigenic and they have immunosuppressive properties. Although liver transplantation is the best way to treat acute and chronic hepatic failure patients, there are several obstacles...
2018: PloS One
https://www.readbyqxmd.com/read/29345290/role-of-nestin-expression-in-angiogenesis-and-breast-cancer-progression
#9
Aleksandra Nowak, Jędrzej Grzegrzółka, Alicja Kmiecik, Aleksandra Piotrowska, Rafał Matkowski, Piotr Dzięgiel
Nestin is an intermediate filament protein and a stem cell marker expressed in several tumours. There is growing evidence of an association between the expression level of nestin and the pathogenesis of triple-negative breast cancer (TNBC). Nestin is also expressed in newly forming tumour vessels and is a valuable marker of ongoing angiogenesis. In this study, we aimed to evaluate the prognostic value of nestin expression in breast tumour cells and to determine whether this expression influences angiogenesis...
February 2018: International Journal of Oncology
https://www.readbyqxmd.com/read/29345053/naringenin-4-5-7-trihydroxyflavanone-suppresses-the-development-of-precancerous-lesions-via-controlling-hyperproliferation-and-inflammation-in-the-colon-of-wistar-rats
#10
Muneeb U Rehman, Manzoor Ur Rahman Mir, Adil Farooq, Shahzada Mudasir Rashid, Bilal Ahmad, Sheikh Bilal Ahmad, Rayeesa Ali, Ishraq Hussain, Mubashir Masoodi, Showkeen Muzamil, Hassan Madkhali, Majid Ahmad Ganaie
Colon cancer is a world-wide health problem and one of the most dangerous type of cancer, affecting both men and women. Naringenin (4, 5, 7-trihydroxyflavanone) is one of the major flavone glycoside present in citrus fruits. Naringenin has long been used in Chinese's traditional medicine because of its exceptional pharmacological properties and non-toxic nature. In the present study, we investigated the chemopreventive potential of Naringenin against 1,2-dimethyhydrazine (DMH)-induced precancerous lesions, that is, aberrant crypt foci (ACF) and mucin depleted foci (MDF), and its role in regulating the oxidative stress, inflammation and hyperproliferation, in the colon of Wistar rats...
January 18, 2018: Environmental Toxicology
https://www.readbyqxmd.com/read/29344992/an-immunohistochemical-score-to-predict-the-outcome-for-oral-squamous-cell-carcinoma
#11
Luís Silva Monteiro, Márcio Diniz-Freitas, Saman Warnakulasuriya, Tomás Garcia-Caballero, Jerónimo Forteza, Máximo Fraga
BACKGROUND: Oral cancer is a major public health problem worldwide, with a poor survival. Our aim was to evaluate several protein markers in oral squamous cell carcinomas (OSCC) and analyse their prognostic value on patient's survival. METHODS: We analysed the expression of EGFR, p53, p27, p16, cyclin D1, cyclin A2, COX-2, Ki-67, Bcl-2, VEGFR-1, and VEGFR-2, by immunohistochemistry on 67 primary OSCC. Cancer-specific survival (CSS) analysis was evaluated by the Cox regression model...
January 18, 2018: Journal of Oral Pathology & Medicine
https://www.readbyqxmd.com/read/29344663/adeno-associated-virus-type-2-mediated-gene-transfer-of-a-short-hairpin-rna-targeting-human-igfbp-2-suppresses-the-proliferation-and-invasion-of-mda-mb-468-cells
#12
Chao Gao, Ru-Song Zhang, Nan Zheng, Chen Wang
Adeno-associated virus 2 (AAV2) is prepotent in the biological treatment of breast tumor because of its low pathogenicity and immunogenicity. Our previous study demonstrated that insulin‑like growth factor‑binding protein 2 (IGFBP‑2) was highly expressed in patients with breast metastasis. In the present study, the effects of recombinant AAV2 on the growth and metastasis of breast cancer cells were determined in vitro, and in vivo. rAAV2-ZsGreen-shRNA-scramble and rAAV2‑ZsGreen‑shRNA‑hIGFBP‑2 were used to transfect MDA‑MB‑468, and MCF‑10A cells respectively, and observed that these virus could not penetrate the normal human breast epithelia MCF‑10A cell line...
January 16, 2018: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29344510/ablation-of-fgfr2-in-fibroblasts-ameliorates-kidney-fibrosis-after-ischemia-reperfusion-injury-in-mice
#13
Zhuo Xu, Chunsun Dai
Background: Fibroblast growth factors (FGFs) are heparin-binding proteins involved in a variety of biological processes. However, the role and mechanisms of FGF/FGFR2 signaling in fibroblast activation and kidney fibrosis need further investigation. Methods: In this study, a mouse model with fibroblast-specific FGFR2 gene disruption was generated. The knockouts were born normal and no kidney dysfunction or histological abnormality was found within 2 months after birth...
December 2017: Kidney Diseases
https://www.readbyqxmd.com/read/29344149/hepatoma-derived-growth-factor-functions-as-an-unfavorable-prognostic-marker-of-human-gliomas
#14
Yang Yang, Shengru Liang, Yuqian Li, Fei Gao, Longlong Zheng, Shilai Tian, Pu Yang, Lihong Li
Hepatoma-derived growth factor (HDGF) regulates various cellular processes involved in the onset and development of tumors. To evaluate the role of HDGF in human gliomas, western blotting analysis, immunohistochemistry staining and reverse transcription-quantitative polymerase chain reaction were performed to detect HDGF protein and mRNA expression levels in glioma and intractable epileptic brain tissue. Various clinicopathological characteristics, including age, gender, World health Organization grade, HDGF expression level, Karnofsky performance Status (KPS) and Ki-67 index were obtained from medical records...
December 2017: Oncology Letters
https://www.readbyqxmd.com/read/29343518/fibroblast-specific-%C3%AE-catenin-signaling-dictates-the-outcome-of-aki
#15
Dong Zhou, Haiyan Fu, Liangxiang Xiao, Hongyan Mo, Hui Zhuo, Xiaojun Tian, Lin Lin, Jianhua Xing, Youhua Liu
AKI is a devastating condition with high morbidity and mortality. The pathologic features of AKI are characterized by tubular injury, inflammation, and vascular impairment. Whether fibroblasts in the renal interstitium have a role in the pathogenesis of AKI is unknown. In this study, we investigated the role of fibroblast-specific β-catenin signaling in dictating the outcome of AKI, using conditional knockout mice in which β-catenin was specifically ablated in fibroblasts (Gli1-β-cat-/-). After ischemia-reperfusion injury (IRI), Gli1-β-cat-/- mice had lower serum creatinine levels and less morphologic injury than Gli1-β-cat+/+ littermate controls...
January 17, 2018: Journal of the American Society of Nephrology: JASN
https://www.readbyqxmd.com/read/29341468/downregulation-of-barh-like-homeobox-2-promotes-cell-proliferation-migration-and-aerobic-glycolysis-through-wnt-%C3%AE-catenin-signaling-and-predicts-a-poor-prognosis-in-non-small-cell-lung-carcinoma
#16
Hao Chen, Maowei Zhang, Wenhui Zhang, Yuanqin Li, Jiechen Zhu, Xiaojiao Zhang, Li Zhao, Shuyang Zhu, Bi Chen
BACKGROUND: Human BarH-like homeobox 2 (Barx2), a homeodomain factor of the Bar family, plays a critical role in cell adhesion and cytoskeleton remodeling, and has been reported in an increasing array of tumor types except non-small cell lung carcinoma (NSCLC). The purpose of the current study was to characterize the expression of Barx2 and assess the clinical significance of Barx2 in NSCLC. METHODS: Quantitative real-time polymerase chain reaction, immunohistochemistry and western blot analysis were used to examine mRNA and protein expression, respectively...
January 17, 2018: Thoracic Cancer
https://www.readbyqxmd.com/read/29340063/a-molecular-signature-of-dormancy-in-cd34-cd38-acute-myeloid-leukaemia-cells
#17
Mazin Gh Al-Asadi, Grace Brindle, Marcos Castellanos, Sean T May, Ken I Mills, Nigel H Russell, Claire H Seedhouse, Monica Pallis
Dormant leukaemia initiating cells in the bone marrow niche are a crucial therapeutic target for total eradication of acute myeloid leukaemia. To study this cellular subset we created and validated an in vitro model employing the cell line TF-1a, treated with Transforming Growth Factor β1 (TGFβ1) and a mammalian target of rapamycin inhibitor. The treated cells showed decreases in total RNA, Ki-67 and CD71, increased aldehyde dehydrogenase activity, forkhead box 03A (FOX03A) nuclear translocation and growth inhibition, with no evidence of apoptosis or differentiation...
December 19, 2017: Oncotarget
https://www.readbyqxmd.com/read/29339715/caveolin-1-inhibits-proliferation-migration-and-invasion-of-human-colorectal-cancer-cells-by-suppressing-phosphorylation-of-epidermal-growth-factor-receptor
#18
Juanli Yang, Tienian Zhu, Ruijing Zhao, Dongmei Gao, Yujie Cui, Kun Wang, Yanli Guo
BACKGROUND Although downregulation of caveolin-1 (Cav-1), which is a key constituent of membrane caveolae and a regulator of cellular processes, is associated with colorectal cancer (CRC), its involvement in the disease progression is largely unknown. This study aimed to explore the role of Cav-1 in CRC and the associated mechanisms. MATERIAL AND METHODS Fresh tissues from patients with CRC and human CRC SW480 cells were used to evaluate Cav-1 and Ki-67 expression using immunohistochemistry and Western blotting...
January 17, 2018: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/29338615/assessment-of-canine-mast-cell-tumor-mortality-risk-based-on-clinical-histologic-immunohistochemical-and-molecular-features
#19
Rodrigo S Horta, Gleidice E Lavalle, Lidianne N Monteiro, Mayara C C Souza, Geovanni D Cassali, Roberto B Araújo
Mast cell tumor (MCT) is a frequent cutaneous neoplasm in dogs that is heterogeneous in clinical presentation and biological behavior, with a variable potential for recurrence and metastasis. Accurate prediction of clinical outcomes has been challenging. The study objective was to develop a system for classification of canine MCT according to the mortality risk based on individual assessment of clinical, histologic, immunohistochemical, and molecular features. The study included 149 dogs with a histologic diagnosis of cutaneous or subcutaneous MCT...
January 1, 2018: Veterinary Pathology
https://www.readbyqxmd.com/read/29338018/oral-mucosa-tissue-gene-expression-profiling-before-during-and-after-radiation-therapy-for-tonsil-squamous-cell-carcinoma
#20
Mette Marcussen, Mads Sønderkær, Julie Støve Bødker, Maria Andersen, Søren Nielsen, Charles Vesteghem, Ilse Christiansen, Olav Jonas Bergmann, Martin Bøgsted, Karen Dybkær, Mogens Vyberg, Hans Erik Johnsen
BACKGROUND: Radiation-therapy (RT) induces mucositis, a clinically challenging condition with limited prophylactic interventions and no predictive tests. In this pilot study, we applied global gene-expression analysis on serial human oral mucosa tissue and blood cells from patients with tonsil squamous cell cancer (TSCC) to identify genes involved in mucositis pathogenesis. METHODS AND FINDINGS: Eight patients with TSCC each provided consecutive buccal biopsies and blood cells before, after 7 days of RT treatment, and 20 days following RT...
2018: PloS One
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