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Nanobiosensor

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https://www.readbyqxmd.com/read/28858858/detection-of-large-deletion-in-human-brca1-gene-in-human-breast-carcinoma-mcf-7-cells-by-using-dna-silver-nanoclusters
#1
Yasaman Sadat Borghei, Morteza Hosseini, Mohammad Reza Ganjali
Here we describe a label-free detection strategy for large deletion mutation in breast cancer related gene BRCA1 based on a DNA-silver Nanocluster Fluorescence upon Recognition-Induced Hybridization. The specific hybridization of DNA templated silver nanoclusters fluorescent probe to target DNAs can act as effective templates for enhancement of AgNCs Fluorescence, which can be used to distinguish the deletion of BRCA1 due to different fluorescent intensities. Under the optimal conditions, the fluorescent intensity of the DNA-AgNCs increased with the increasing deletion type within a dynamic range from 1...
August 31, 2017: Methods and Applications in Fluorescence
https://www.readbyqxmd.com/read/28840718/detection-and-quantification-of-biologically-active-botulinum-neurotoxin-serotypes-a-and-b-using-a-f%C3%A3-rster-resonance-energy-transfer-based-quantum-dot-nanobiosensor
#2
Yun Wang, H Christopher Fry, Guy E Skinner, Kristin M Schill, Timothy V Duncan
Botulinum neurotoxin (BoNT) is the most potent toxin known. The ingestion of food contaminated with biologically active BoNT causes foodborne botulism, which can lead to respiratory paralysis, coma, and death after ingestion of as little as 70 μg for a 70 kg human. Because of its lethality and challenges associated with current detection methods, there is an urgent need for highly sensitive rapid screening techniques capable of detecting biologically active BoNT. Here, we describe a Förster resonance energy transfer-based nanobiosensor that uses quantum dots (QDs) and two specific quencher-labeled peptide probes to detect and differentiate two biologically active forms of BoNT, serotypes A and B, which were responsible for 80% of human foodborne botulism cases in the U...
September 20, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28814074/multifunctional-yolk-shell-nanostructure-as-a-superquencher-for-fluorescent-analysis-of-potassium-ion-using-guanine-rich-oligonucleotides
#3
Na Lu, Yanli Wen, Gang Liu, Lei Ding, Caixia Zeng, Ali Aldalbahi, Muhammad Naziruddin Khan, Govindasami Periyasami, Mostafizur Rahaman, Abdulaziz Alrohaili, Jiye Shi, Shiping Song, Lihua Wang, Min Zhang
The excellent performance of a biosensor generally depends on the high signal-to-noise ratio, and the superquencher plays a dominant role in fluorescent sensors. Novel nanoquenchers exhibited high quenching efficiency in various fluorescent assays of biological/chemical molecules. Here, we developed a novel nano-biosensor using Fe3O4@C yolk-shell nanoparticles (YSNPs) and studied their quenching effect. We found Fe3O4@C YSNP was a superquencher and exhibited an ultrastrong quenching ability, up to almost 100% quenching efficiency, toward fluorophores...
September 13, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28724346/biosensing-technologies-for-therapeutic-drug-monitoring
#4
Anna Meneghello, Stefano Tartaggia, Maria Domenica Alvau, Federico Polo, Giuseppe Toffoli
Therapeutic drug monitoring (TDM) is the clinical practice of measuring pharmaceutical drug concentrations in patients' biofluids at designated intervals to allow a close and timely control of their dosage. This practice allows for rapid medical intervention in case of toxicity-related issues and/or adjustment of dosage to better fit the therapeutic demand. Currently, TDM is performed in centralized laboratories employing instruments, such as immunoassay analyzers and mass spectrometers that can be run only by trained personnel...
July 20, 2017: Current Medicinal Chemistry
https://www.readbyqxmd.com/read/28721039/top-down-nanofabrication-of-silicon-nanoribbon-field-effect-transistor-si-nr-fet-for-carcinoembryonic-antigen-detection
#5
Zengtao Bao, Jialin Sun, Xiaoqian Zhao, Zengyao Li, Songkui Cui, Qingyang Meng, Ye Zhang, Tong Wang, Yanfeng Jiang
Sensitive and quantitative detection of tumor markers is highly required in the clinic for cancer diagnosis and consequent treatment. A field-effect transistor-based (FET-based) nanobiosensor emerges with characteristics of being label-free, real-time, having high sensitivity, and providing direct electrical readout for detection of biomarkers. In this paper, a top-down approach is proposed and implemented to fulfill a novel silicon nano-ribbon FET, which acts as biomarker sensor for future clinical application...
2017: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/28629010/fabrication-of-dna-nanotubes-with-an-array-of-exterior-magnetic-nanoparticles
#6
Adele Rafati, Ali Zarrabi, Pooria Gill
Described here a methodology for arraying of magnetic nanoparticles (MNPs) on the surface of DNA nanotubes (DNTs). Positioning of magnetic nanoparticles at exterior surface of DNTs were shaped after self-assembling of oligonucleotide staples within an M13mp18 DNA scaffold via an origami process. The staples were partially labeled with biotin to be arrayed at the surface of DNTs. Gel retardation assay of the DNTs carrying magnetic nanoparticles indicated a reversely behavioral electrophoretic movement in comparison to the nanotubes have been demonstrated previously...
October 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28623816/siderophore-coated-magnetic-iron-nanoparticles-rational-designing-of-water-soluble-nanobiosensor-for-visualizing-al-3-in-live-organism
#7
M Raju, Sakshi Srivastava, Ratish R Nair, Ishan H Raval, Soumya Haldar, Pabitra B Chatterjee
This article aims to establish the judicious use of iron-binding chemistry of microbial chelators in order to functionalize the surface of iron nanoparticles to develop non-toxic nanobiosensor. Anchoring a simple siderophore 2,3-dihydroxybenzoylglycine (H3L), which bears catechol and carboxyl functionalities in tandem, on to the surface of Fe3O4 nanoparticles has developed a unique nanobiosensor HL-FeNPs which showed highly selective and sensitive detection of Al(3+) in 100% water at physiological pH. The biosensor HL-FeNPs, with 20nM limit of detection, behaves reversibly and instantly...
June 8, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28585972/plasmonic-nanohole-array-biosensor-for-label-free-and-real-time-analysis-of-live-cell-secretion
#8
Xiaokang Li, Maria Soler, Cenk I Özdemir, Alexander Belushkin, Filiz Yesilköy, Hatice Altug
Cell secretion dynamics plays a central role in physiological and disease processes. Due to its various temporal profiles, it is essential to implement a precise detection scheme for continuous monitoring of secretion in real time. The current fluorescent and colorimetric approaches hinder such applications due to their multiple time-consuming steps, molecular labeling, and especially the 'snapshot' endpoint readouts. Here, we develop a nanoplasmonic biosensor for real-time monitoring of live cell cytokine secretion in a label-free configuration...
June 6, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/28576073/zno-nanoflower-based-sensitive-nano-biosensor-for-amyloid-detection
#9
Najim Akhtar, Sanjay Kisan Metkar, Agnishwar Girigoswami, Koyeli Girigoswami
Zinc oxide (ZnO) is a semiconductor metal oxide nanoparticle with inherent optical properties. Among the different zinc oxide nanostructures, nanoflowers have greater surface area. Utilizing this property a reagentless biosensor has been developed for the detection of beta amyloids, a hallmark of neurodegenerative diseases like Alzheimer's disease, Creutzfeldt-Jakob Syndrome, insulin dependent type II diabetes etc. The poor fluorescence quantum yield and photobleaching effect of Thioflavin T (ThT) upon binding to the model insulin amyloid beta sheets in solution can be overcome by the present engineered biosensor where ThT acts as a target as well as a reporter to detect amyloids adsorbed on a solid template based on ZnO nanoflower...
September 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28487527/development-of-frequency-based-taste-receptors-using-bioinspired-glucose-nanobiosensor
#10
Amin TermehYousefi, Katsumi Tateno, Samira Bagheri, Hirofumi Tanaka
A method to fabricate a bioinspired nanobiosensor using electronic-based artificial taste receptors for glucose diagnosis is presented. Fabricated bioinspired glucose nanobiosensor designated based on an artificial taste bud including an amperometric glucose biosensor and taste bud-inspired circuits. In fact, the design of the taste bud-inspired circuits was inspired by the signal-processing mechanism of taste nerves which involves two layers. The first, known as a type II cell, detects the glucose by glucose oxidase and transduces the current signal obtained for the pulse pattern is conducted to the second layer, called type III cell, to induce synchronisation of the neural spiking activity...
May 9, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28470301/notch-signaling-in-regulating-angiogenesis-in-a-3d-biomimetic-environment
#11
Yi Zheng, Shue Wang, Xufeng Xue, Alan Xu, Wei Liao, Alice Deng, Guohao Dai, Allen P Liu, Jianping Fu
Angiogenesis is a complex cellular process involving highly orchestrated invasion and organization of endothelial cells (ECs) in a three-dimensional (3D) environment. Recent evidence indicates that Notch signaling is critically involved in regulating specialized functions and distinct fates of ECs in newly formed vasculatures during angiogenesis. Here, we demonstrated, for the first time, the application of a microengineered biomimetic system to quantitatively investigate the role of Notch signaling in regulating early angiogenic sprouting and vasculature formation of ECs in a 3D extracellular matrix...
May 31, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/28414950/a-nanobiosensor-composed-of-exfoliated-graphene-oxide-and-gold-nano-urchins-for-detection-of-gmo-products
#12
Zahra Aghili, Navid Nasirizadeh, Adeleh Divsalar, Shahram Shoeibi, Parichehreh Yaghmaei
Genetically Modified Organisms, have been entered our food chain and detection of these organisms in market products are still the main challenge for scientists. Among several developed detection/quantification methods for detection of these organisms, the electrochemical nanobiosensors are the most attended which are combining the advantages of using nanomaterials, electrochemical methods and biosensors. In this research, a novel and sensitive electrochemical nanobiosensor for detection/quantification of these organisms have been developed using nanomaterials; Exfoliated Graphene Oxide and Gold Nano-Urchins for modification of the screen-printed carbon electrode, and also applying a specific DNA probe as well as hematoxylin for electrochemical indicator...
September 15, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28335308/probe-microscopic-studies-of-dna-molecules-on-carbon-nanotubes
#13
REVIEW
Kazuo Umemura, Katsuki Izumi, Shusuke Oura
Hybrids of DNA and carbon nanotubes (CNTs) are promising nanobioconjugates for nanobiosensors, carriers for drug delivery, and other biological applications. In this review, nanoscopic characterization of DNA-CNT hybrids, in particular, characterization by scanning probe microscopy (SPM), is summarized. In many studies, topographical imaging by atomic force microscopy has been performed. However, some researchers have demonstrated advanced SPM operations in order to maximize its unique and valuable functions...
October 8, 2016: Nanomaterials
https://www.readbyqxmd.com/read/28276344/a-new-fluorescence-turn-on-nanobiosensor-for-the-detection-of-micro-rna-21-based-on-a-dna-gold-nanocluster
#14
Morteza Hosseini, Elnaz Ahmadi, Yasaman-Sadat Borghei, Mohammad Reza Ganjali
In this study, DNA/gold nanoclusters (AuNCs) were used to develop an AuNC-based turn-on fluorescence probe for the analysis of mi-RNA-21, which is a potential screening biomarker for cancer detection. AuNCs on a DNA scaffold were prepared through a one-pot wet-chemical route and evaluated by transmission electron microscopy and dynamic light scattering. Experiments revealed that the fluorescence intensity of the DNA-AuNCs showed a gradual increase with the addition of the target species in a concentration range from 1pM to 10 nM...
March 8, 2017: Methods and Applications in Fluorescence
https://www.readbyqxmd.com/read/28120920/analysis-of-alternative-splicing-events-for-cancer-diagnosis-using-a-multiplexing-nanophotonic-biosensor
#15
César S Huertas, Santos Domínguez-Zotes, Laura M Lechuga
Personalized medicine is a promising tool not only for prevention, screening and development of more efficient treatment strategies, but also for diminishing the side effects caused by current therapies. Deciphering gene regulation pathways provides a reliable prognostic analysis to elucidate the origin of grave diseases and facilitate the selection of the most adequate treatment for each individual. Alternative splicing of mRNA precursors is one of these gene regulation pathways and enables cells to generate different protein outputs from the same gene depending on their developmental or homeostatic status...
January 25, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28107978/fret-based-nanobiosensor-for-detection-of-scopolamine-in-hairy-root-extraction-of-atropa-belladonna
#16
Fereshte Bagheri, Khosro Piri, Afshine Mohsenifar, Smaiil Ghaderi
A simple, sensitive, selective, and rapid optical nanobiosensor based on FRET was designed to detect tropane alkaloids as anti-cholinergic agents in natural and transgenic hairy roots extracts of Atropa belladonna. To achieve that, conjugation of tioglycolyic acid capped cadmium telluride quantum Dots, M2 muscarinic receptor (Cd/Te QDs-M2R) and conjugation of scopolamine-rhodamine123 (Sc-Rho123) were performed. More specifically, proportional amounts of M2 muscarinic receptor and quantum dots (QDs) were conjugated while scopolamine (as a tropane alkaloid) and rhodamine123 were also combined and these moieties functioned as donor and acceptor pairs, respectively...
March 1, 2017: Talanta
https://www.readbyqxmd.com/read/28081500/a-highly-sensitive-quantum-dots-dna-nanobiosensor-based-on-fluorescence-resonance-energy-transfer-for-rapid-detection-of-nanomolar-amounts-of-human-papillomavirus-18
#17
Mojtaba Shamsipur, Vahid Nasirian, Kamran Mansouri, Ali Barati, Asad Veisi-Raygani, Soheila Kashanian
A very sensitive and convenient nanobiosensor based on fluorescence resonance energy transfer (FRET) was developed for the detection of a 22-mer oligonucleotides sequence in Human Papillomavirus 18 virus (HPV18) gene. For this purpose, water-soluble CdTe quantum dots (QDs) were synthesized and, subsequently, amino-modified 11-mer oligonucleotide as one of the two necessary probes was attached to QDs surface to form functional QDs-DNA conjugates. Right after addition of the QDs-DNA and a second Cyanine5 (Cy5)-labeled 11-mer oligonucleotide probe to the DNA target solution, the sandwiched hybrids were formed...
March 20, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28033555/unbinding-pathway-energy-of-glyphosate-from-the-epsps-enzyme-binding-site-characterized-by-steered-molecular-dynamics-and-potential-of-mean-force
#18
Moacir F Ferreira, Eduardo F Franca, Fábio L Leite
The quantification of herbicides in the environment, like glyphosate, is extremely important to prevent contamination. Nanobiosensors stands out in the quantization process, because of the high selectivity, sensitivity and short response time of the method. In order to emulate the detection of glyphosate using a specific nanobiossensor through an Atomic Force Microscope (AFM), this work carried out Steered Molecular Dynamics simulations (SMD) in which the herbicide was unbinded from the active site of the enzyme 5- enolpyruvylshikimate 3 phosphate synthase (EPSPS) along three different directions...
March 2017: Journal of Molecular Graphics & Modelling
https://www.readbyqxmd.com/read/28002949/comprehensive-study-of-the-effects-of-nanopore-structures-on-enzyme-activity-for-the-enzyme-based-electrochemical-biosensors-based-on-molecular-simulation
#19
Guang Yang, Bo Liang, Qin Zhu, Yichuan Hu, Xuesong Ye
Assembly of biocompatible nanostructures to retain the enzyme activity and improve the biocatalytic ability is a decisive factor for enhancing the performance of enzyme biosensors. However, there is still a lack of molecular level understandings of the physicochemical interaction mechanism at the interface of biosensor electrodes and enzymes. Here, for the first time at molecular level, the effects of two classic biosensor electrode materials with different electrical properties and morphologies and glucose oxidase (GOD) on retaining the enzyme conformation were analyzed by molecular dynamics simulation...
December 22, 2016: Journal of Physical Chemistry. A
https://www.readbyqxmd.com/read/27995447/ethics-nanobiosensors-and-elite-sport-the-need-for-a-new-governance-framework
#20
Robert Evans, Michael McNamee, Owen Guy
Individual athletes, coaches and sports teams seek continuously for ways to improve performance and accomplishment in elite competition. New techniques of performance analysis are a crucial part of the drive for athletic perfection. This paper discusses the ethical importance of one aspect of the future potential of performance analysis in sport, combining the field of biomedicine, sports engineering and nanotechnology in the form of 'Nanobiosensors'. This innovative technology has the potential to revolutionise sport, enabling real time biological data to be collected from athletes that can be electronically distributed...
December 19, 2016: Science and Engineering Ethics
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