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Mehdi Yoosefian, Nazanin Etminan
We have designed a novel nanobiosensor for in silico detecting proteins based on leucine/Pd-loaded single-walled carbon nanotube matrix. Density functional theory at the B3LYP/6-31G (d) level of theory was realized to analyze the geometrical and electronic structure of the proposed nanobiosensor. The solvent effects were investigated using the Tomasi's polarized continuum model. Atoms-in-molecules theory was used to study the nature of interactions by calculating the electron density ρ(r) and Laplacian at the bond critical points...
March 14, 2018: Amino Acids
Ying Zhang, Zhenhua Shuai, Hao Zhou, Zhimin Luo, Bing Liu, Yinan Zhang, Lei Zhang, Shufen Chen, Jie Chao, Lixing Weng, Quli Fan, Chunhai Fan, Wei Huang, Lianhui Wang
Analysis of biomolecules at the single-molecule level is a great challenge in molecular diagnostics, gene profiling, and environmental monitoring. In this work, we designed a smart plasmonic nanobiosensor based on individual Au@Ag core-shell nanocube (Au@Ag NC) modified with tetrahedron-structured DNA (tsDNA), which is capable of detecting microRNA 21 (miR-21) at the single-molecule level. An average localized surface plasmon resonance (LSPR) scattering spectral wavelength shift of approximately 0.4 nm was obtained for a single miR-21 hybridization event on the nanobiosensor...
March 5, 2018: Journal of the American Chemical Society
Kuldeep Mahato, Pawan Kumar Maurya, Pranjal Chandra
Among various problems faced by mankind, health-related concerns are prevailing since long which are commonly found in the form of infectious diseases and different metabolic disorders. The clinical cure and management of such abnormalities are greatly dependent on the availability of their diagnoses. The conventional diagnostics used for such purposes are extremely powerful; however, most of these are limited by time-consuming protocols and require higher volume of test sample, etc. A new evolving technology called "biosensor" in this context shows an enormous potential for an alternative diagnostic device, which constantly compliments the conventional diagnoses...
March 2018: 3 Biotech
Behrooz Shojaee Sadi, Mansour Bayat, Parviz Tajik, Seyed Jamal Hashemi
The specific immune-reaction between the anti-citrinin antibody immobilized on the surface of magnetic/silica core-shell (MSCS) and the citrinin-Rho123-BSA conjugate brings the Rho123 fluorophore as an acceptor and the QDs as a donor in close spatial proximity and causes FRET for occurring upon photo-excitation of the QDs. The novelties of this study include: (1) immobilization of the MSCS; (2) large amount of the immobilized QDs, and (3) immobilization of a large amount of Rho123 on the BSA macromolecule. Cd/Te QDs were synthesized by the simultaneous reduction of cadmium chloride and tellurium in the presence of sodium borohydride...
January 2018: Saudi Journal of Biological Sciences
Janine Martinazzo, Daniela K Muenchen, Alexandra N Brezolin, Alana M Cezaro, Aline A Rigo, Alexandra Manzoli, Lucélia Hoehne, Fábio L Leite, Juliana Steffens, Clarice Steffens
The aim of this study was to develop a cantilever nanobiosensor for atrazine detection in liquid medium by immobilising the biological recognition element (tyrosinase vegetal extract) on its surface with self-assembled monolayers using gold, 16-mercaptohexadecanoic acid, 1-ethyl-3-[3-dimethylaminopropyl] carbodiimide hydrochloride/n-hydroxysuccinimide. Cantilever nanobiosensors presented a surface compression tension increase when atrazine concentrations were increased, with a limit of detection and limit of quantification of 7...
January 10, 2018: Journal of Environmental Science and Health. Part. B, Pesticides, Food Contaminants, and Agricultural Wastes
Maria Sofia Falzarano, Cristina Flesia, Roberta Cavalli, Caterina Guiot, Alessandra Ferlini
Background Genetic alterations cause hereditary diseases (HDs) with a wide range of incidences. Some, like cystic fibrosi, occur frequently (1/1,000 newborns), whilst others, such as Pompe disease and other metabolic disorders are very rare (1/100,000 newborns). They are well under the threshold of 1/3,000, denoted by the European Community as rare diseases (RDs). Genetic alterations are also associated with multifactorial disorders like diabetes, and underline both somatic and germline mutations in cancer...
January 10, 2018: Current Pharmaceutical Design
Mohammad Rizwan, Noor Faizah Mohd-Naim, Minhaz Uddin Ahmed
The rapid and increasing use of the nanomaterials (NMs), nanostructured materials (NSMs), metal nanoclusters (MNCs) or nanocomposites (NCs) in the development of electrochemiluminescence (ECL) nanobiosensors is a significant area of study for its massive potential in the practical application of nanobiosensor fabrication. Recently, NMs or NSMs (such as AuNPs, AgNPs, Fe₃O₄, CdS QDs, OMCs, graphene, CNTs and fullerenes) or MNCs (such as Au, Ag, and Pt) or NCs of both metallic and non-metallic origin are being employed for various purposes in the construction of biosensors...
January 9, 2018: Sensors
Amir Syahir, Kotaro Kajikawa, Hisakazu Mihara
Direct bio-monitoring essentially involves optical means since the photon has insignificant effects over biomolecules. Over the years, the laser induced surface plasmon resonance method with various modifications as well as versatile localized plasmon excited by incoherent light have facilitated in recording many nanobiological activities. Yet monitoring interactions of small molecules including drugs requires signal amplification and improvement on signal-to-noise ratio. This paper focused on how the refractive index based nanobio-sensoring gold platform can produce more efficient, adaptable and more practical detection techniques to observe molecular interactions at a high degree of sensitivity...
December 13, 2017: Protein and Peptide Letters
Zahra Aghili, Navid Nasirizadeh, Adeleh Divsalar, Shahram Shoeibi, Parichehreh Yaghmaei
Early detection of Parkinson's disease (PD), as a dangerous neurodegenerative disease, is a key factor in the therapy or prevention of further development of this disease. We developed an electrochemical nanobiosensor for early detection of PD based on the quantification of circulating biomarker, miR-195. Exfoliated graphene oxide (EGO) and gold nanowires (GNWs) were used to modify the surface of screen-printed carbon electrode. A single-strand thiolated probe was designed for specific hybridization with target miRNA (miR-195), and doxorubicin was used as an electrochemical indicator for differential pulse voltammetry measurements...
December 7, 2017: Artificial Cells, Nanomedicine, and Biotechnology
Alireza Abi, Zahra Mohammadpour, Xiaolei Zuo, Afsaneh Safavi
The detection of biomarkers using sensitive and selective analytical devices is critically important for the early stage diagnosis and treatment of diseases. The synergy between the high specificity of nucleic acid recognition units and the great sensitivity of electrochemical signal transductions has already shown promise for the development of efficient biosensing platforms. Yet nucleic-acid based electrochemical biosensors often rely on target amplification strategies (e.g., polymerase chain reactions) to detect analytes at clinically relevant concentration ranges...
April 15, 2018: Biosensors & Bioelectronics
Shue Wang, Yuan Xiao, Donna D Zhang, Pak Kin Wong
Transcription and translation are under tight spatiotemporal regulation among cells to coordinate multicellular organization. Methods that allow massively parallel detection of gene expression dynamics at the single cell level are required for elucidating the complex regulatory mechanisms. Here we present a multiplex nanobiosensor for real-time monitoring of protein and mRNA expression dynamics in live cells based on gapmer aptamers and complementary locked nucleic acid probes. Using the multiplex nanobiosensor, we quantified spatiotemporal dynamics of vascular endothelial growth factor A mRNA and protein expressions in single human endothelial cells during microvascular self-organization...
February 2018: Biomaterials
Daniel Wasik, Ashok Mulchandani, Marylynn V Yates
Dengue virus (DENV) and its primary mosquito vectors Aedes spp. have spread to every continent except Antarctica, causing outbreaks and autochthonous transmission in previously disease-free regions. Recently, the spread of other arboviruses carried by invasive Aedes spp., such as Chikungunya and Zika, seem to be following similar trends as DENV and have renewed interest in monitoring and modeling arboviruses within mosquito vectors. Unfortunately, current commercially available detection methods are designed for the diagnosis of DENV in humans or are too expensive and complex for sustainable monitoring...
January 2, 2018: Analytical Chemistry
Chiara Bedin, Sara Crotti, Ennio Tasciotti, Marco Agostini
Nowadays, fast and sensitive methods for biomarkers detection exist, but the performance of most of them still rely in centralized laboratory testing. The development of small, fast and simple to use medical device that can help in making diagnosis accurate and with low-invasiveness is now a major challenge for nanotechnology. To develop diagnostic devices for cancer detection, nanomaterials-based systems have significant advantages over current conventional approaches in terms of simplicity, sensitivity, specificity and rapidity...
November 16, 2017: Current Medicinal Chemistry
Alireza Gharatape, Ahmad Yari Khosroushahi
Thousands of billions of dollars have been disbursed worldwide for the diagnosis and therapy of disease. Nanobioscience combined with sensing technology has facilitated the pathway to diagnosis by introducing a novel, highly sensitive and selective detection system known as the nanobiosensor to reduce the overall cost of treatment. Among the many types of biosensors, optical ones are more easily developed because of their low cost, rapid construction, small size, high performance, high sensitivity and selectivity, high signal-to-noise ratio, flexible geometry, and portability properties...
September 29, 2017: Applied Immunohistochemistry & Molecular Morphology: AIMM
Hadeel Elayan, Pedram Johari, Raed M Shubair, Josep Miquel Jornet
Wireless communication among implanted nanobiosensors will enable transformative smart health monitoring and diagnosis systems. The state of the art of nano-electronics and nano-photonics points to the Terahertz (THz) band (0.1-10 THz) and the optical frequency bands (infrared, 30-400 THz, and visible, 400-750 THz) as the frequency range for communication among nano-biosensors. Recently, several propagation models have been developed to study and assess the feasibility of intrabody electromagnetic (EM) nanoscale communication...
September 28, 2017: IEEE Transactions on Nanobioscience
Richard Leggett, Paul Thomas, María J Marín, Jelena Gavrilovic, David A Russell
Nitric oxide (NO) plays an essential role within the immune system since it is involved in the break-down of infectious agents such as viruses and bacteria. The ability to measure the presence of NO in the intracellular environment would provide a greater understanding of the pathophysiological mechanism of this important molecule. Here we report the detection of NO from the intracellular phagolysosome using a fluorescently tagged metalloprotein-gold nanoparticle conjugate. The metalloprotein cytochrome c, fluorescently tagged with an Alexa Fluor dye, was self-assembled onto gold nanoparticles to produce a NO specific nanobiosensor...
October 23, 2017: Analyst
Kuldeep Mahato, Ashutosh Kumar, Pawan Kumar Maurya, Pranjal Chandra
Cancer is one of leading causes of death in the world and occurs in more than two hundred types according to the National Cancer Institute. Its early diagnosis has been remained a prime focus amongst scientists and clinicians since long, not only to understand the complications but also to mitigate its chance of further proliferation. Nowadays, tremendous advances in nanotechnology-empowered diagnostics are serving a substantial input to identify biomarkers associated with various cancers. These biomarkers are found in different forms including overexpressed proteins/surface antigens, metabolites, miRNA, and the entire cell as well...
September 5, 2017: Biosensors & Bioelectronics
Saeromi Chung, Pranjal Chandra, Jaseok Peter Koo, Yoon-Bo Shim
Highly sensitive detection of chemokines in various biological matrices and its interaction with a natural receptor molecule has tremendous importance in cell signaling, medical diagnostics, and therapeutics. In this direction, we have designed the first bifunctional nanobiosensor for chemokine screening and detection in a single experimental setting. The sensor probe was fabricated by immobilizing CXCR2 on the gold nanoparticles (AuNPs) deposited 2,2':5',2''-terthiophene-3' (p-benzoic acid) (TBA) nanocomposite film...
September 20, 2017: Biosensors & Bioelectronics
Sathish Sugumaran, Mohd Faizal Jamlos, Mohd Noor Ahmad, Chandar Shekar Bellan, Dominique Schreurs
Early cancer detection and treatment is an emerging and fascinating field of plasmonic nanobiosensor research. It paves to enrich a life without affecting living cells leading to a possible survival of the patient. This review describes a past and future prospect of an integrated research field on nanostructured metamaterials, microwave transmission, surface plasmonic resonance, nanoantennas, and their manifested versatile properties with nano-biosensors towards early cancer detection to preserve human health...
August 24, 2017: Biosensors & Bioelectronics
Yasaman Sadat Borghei, Morteza Hosseini, Mohammad Reza Ganjali
Here we describe a label-free detection strategy for large deletion mutation in breast cancer related gene BRCA1 based on a DNA-silver Nanocluster Fluorescence upon Recognition-Induced Hybridization. The specific hybridization of DNA templated silver nanoclusters fluorescent probe to target DNAs can act as effective templates for enhancement of AgNCs Fluorescence, which can be used to distinguish the deletion of BRCA1 due to different fluorescent intensities. Under the optimal conditions, the fluorescent intensity of the DNA-AgNCs increased with the increasing deletion type within a dynamic range from 1...
August 31, 2017: Methods and Applications in Fluorescence
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