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Janine Martinazzo, Daniela K Muenchen, Alexandra N Brezolin, Alana M Cezaro, Aline A Rigo, Alexandra Manzoli, Lucélia Hoehne, Fábio L Leite, Juliana Steffens, Clarice Steffens
The aim of this study was to develop a cantilever nanobiosensor for atrazine detection in liquid medium by immobilising the biological recognition element (tyrosinase vegetal extract) on its surface with self-assembled monolayers using gold, 16-mercaptohexadecanoic acid, 1-ethyl-3-[3-dimethylaminopropyl] carbodiimide hydrochloride/n-hydroxysuccinimide. Cantilever nanobiosensors presented a surface compression tension increase when atrazine concentrations were increased, with a limit of detection and limit of quantification of 7...
January 10, 2018: Journal of Environmental Science and Health. Part. B, Pesticides, Food Contaminants, and Agricultural Wastes
Maria Sofia Falzarano, Cristina Flesia, Roberta Cavalli, Caterina Guiot, Alessandra Ferlini
Background Genetic alterations cause hereditary diseases (HDs) with a wide range of incidences. Some, like cystic fibrosi, occur frequently (1/1,000 newborns), whilst others, such as Pompe disease and other metabolic disorders are very rare (1/100,000 newborns). They are well under the threshold of 1/3,000, denoted by the European Community as rare diseases (RDs). Genetic alterations are also associated with multifactorial disorders like diabetes, and underline both somatic and germline mutations in cancer...
January 10, 2018: Current Pharmaceutical Design
Mohammad Rizwan, Noor Faizah Mohd-Naim, Minhaz Uddin Ahmed
The rapid and increasing use of the nanomaterials (NMs), nanostructured materials (NSMs), metal nanoclusters (MNCs) or nanocomposites (NCs) in the development of electrochemiluminescence (ECL) nanobiosensors is a significant area of study for its massive potential in the practical application of nanobiosensor fabrication. Recently, NMs or NSMs (such as AuNPs, AgNPs, Fe₃O₄, CdS QDs, OMCs, graphene, CNTs and fullerenes) or MNCs (such as Au, Ag, and Pt) or NCs of both metallic and non-metallic origin are being employed for various purposes in the construction of biosensors...
January 9, 2018: Sensors
Amir Syahir, Kotaro Kajikawa, Hisakazu Mihara
Direct bio-monitoring essentially involves optical means since the photon has insignificant effects over biomolecules. Over the years, the laser induced surface plasmon resonance method with various modifications as well as versatile localized plasmon excited by incoherent light have facilitated in recording many nanobiological activities. Yet monitoring interactions of small molecules including drugs requires signal amplification and improvement on signal-to-noise ratio. This paper focused on how the refractive index based nanobio-sensoring gold platform can produce more efficient, adaptable and more practical detection techniques to observe molecular interactions at a high degree of sensitivity...
December 13, 2017: Protein and Peptide Letters
Zahra Aghili, Navid Nasirizadeh, Adeleh Divsalar, Shahram Shoeibi, Parichehreh Yaghmaei
Early detection of Parkinson's disease (PD), as a dangerous neurodegenerative disease, is a key factor in the therapy or prevention of further development of this disease. We developed an electrochemical nanobiosensor for early detection of PD based on the quantification of circulating biomarker, miR-195. Exfoliated graphene oxide (EGO) and gold nanowires (GNWs) were used to modify the surface of screen-printed carbon electrode. A single-strand thiolated probe was designed for specific hybridization with target miRNA (miR-195), and doxorubicin was used as an electrochemical indicator for differential pulse voltammetry measurements...
December 7, 2017: Artificial Cells, Nanomedicine, and Biotechnology
Alireza Abi, Zahra Mohammadpour, Xiaolei Zuo, Afsaneh Safavi
The detection of biomarkers using sensitive and selective analytical devices is critically important for the early stage diagnosis and treatment of diseases. The synergy between the high specificity of nucleic acid recognition units and the great sensitivity of electrochemical signal transductions has already shown promise for the development of efficient biosensing platforms. Yet nucleic-acid based electrochemical biosensors often rely on target amplification strategies (e.g., polymerase chain reactions) to detect analytes at clinically relevant concentration ranges...
November 4, 2017: Biosensors & Bioelectronics
Shue Wang, Yuan Xiao, Donna D Zhang, Pak Kin Wong
Transcription and translation are under tight spatiotemporal regulation among cells to coordinate multicellular organization. Methods that allow massively parallel detection of gene expression dynamics at the single cell level are required for elucidating the complex regulatory mechanisms. Here we present a multiplex nanobiosensor for real-time monitoring of protein and mRNA expression dynamics in live cells based on gapmer aptamers and complementary locked nucleic acid probes. Using the multiplex nanobiosensor, we quantified spatiotemporal dynamics of vascular endothelial growth factor A mRNA and protein expressions in single human endothelial cells during microvascular self-organization...
November 24, 2017: Biomaterials
Daniel Aaron Wasik, Ashok Mulchandani, Marylynn V Yates
Dengue virus (DENV) and its primary mosquito vectors Aedes spp., have spread to every continent except Antarctica, causing outbreaks and autochthonous transmission in previously disease-free regions. Recently, the spread of other arboviruses carried by invasive Aedes spp., such as Chikungunya and Zika, seem to be following similar trends as DENV and have renewed interest in monitoring and modelling arboviruses within mosquito vectors. Unfortunately, current commercially available detection methods are designed for the diagnosis of DENV in humans or are too expensive and complex for sustainable monitoring...
November 28, 2017: Analytical Chemistry
Chiara Bedin, Sara Crotti, Ennio Tasciotti, Marco Agostini
Nowadays, fast and sensitive methods for biomarkers detection exist, but the performance of most of them still rely in centralized laboratory testing. The development of small, fast and simple to use medical device that can help in making diagnosis accurate and with low-invasiveness is now a major challenge for nanotechnology. To develop diagnostic devices for cancer detection, nanomaterials-based systems have significant advantages over current conventional approaches in terms of simplicity, sensitivity, specificity and rapidity...
November 16, 2017: Current Medicinal Chemistry
Alireza Gharatape, Ahmad Yari Khosroushahi
Thousands of billions of dollars have been disbursed worldwide for the diagnosis and therapy of disease. Nanobioscience combined with sensing technology has facilitated the pathway to diagnosis by introducing a novel, highly sensitive and selective detection system known as the nanobiosensor to reduce the overall cost of treatment. Among the many types of biosensors, optical ones are more easily developed because of their low cost, rapid construction, small size, high performance, high sensitivity and selectivity, high signal-to-noise ratio, flexible geometry, and portability properties...
September 29, 2017: Applied Immunohistochemistry & Molecular Morphology: AIMM
Hadeel Elayan, Pedram Johari, Raed M Shubair, Josep Miquel Jornet
Wireless communication among implanted nanobiosensors will enable transformative smart health monitoring and diagnosis systems. The state of the art of nano-electronics and nano-photonics points to the Terahertz (THz) band (0.1-10 THz) and the optical frequency bands (infrared, 30-400 THz, and visible, 400-750 THz) as the frequency range for communication among nano-biosensors. Recently, several propagation models have been developed to study and assess the feasibility of intrabody electromagnetic (EM) nanoscale communication...
September 28, 2017: IEEE Transactions on Nanobioscience
Richard Leggett, Paul Thomas, María J Marín, Jelena Gavrilovic, David A Russell
Nitric oxide (NO) plays an essential role within the immune system since it is involved in the break-down of infectious agents such as viruses and bacteria. The ability to measure the presence of NO in the intracellular environment would provide a greater understanding of the pathophysiological mechanism of this important molecule. Here we report the detection of NO from the intracellular phagolysosome using a fluorescently tagged metalloprotein-gold nanoparticle conjugate. The metalloprotein cytochrome c, fluorescently tagged with an Alexa Fluor dye, was self-assembled onto gold nanoparticles to produce a NO specific nanobiosensor...
October 23, 2017: Analyst
Kuldeep Mahato, Ashutosh Kumar, Pawan Kumar Maurya, Pranjal Chandra
Cancer is one of leading causes of death in the world and occurs in more than two hundred types according to the National Cancer Institute. Its early diagnosis has been remained a prime focus amongst scientists and clinicians since long, not only to understand the complications but also to mitigate its chance of further proliferation. Nowadays, tremendous advances in nanotechnology-empowered diagnostics are serving a substantial input to identify biomarkers associated with various cancers. These biomarkers are found in different forms including overexpressed proteins/surface antigens, metabolites, miRNA, and the entire cell as well...
September 5, 2017: Biosensors & Bioelectronics
Saeromi Chung, Pranjal Chandra, Jaseok Peter Koo, Yoon-Bo Shim
Highly sensitive detection of chemokines in various biological matrices and its interaction with a natural receptor molecule has tremendous importance in cell signaling, medical diagnostics, and therapeutics. In this direction, we have designed the first bifunctional nanobiosensor for chemokine screening and detection in a single experimental setting. The sensor probe was fabricated by immobilizing CXCR2 on the gold nanoparticles (AuNPs) deposited 2,2':5',2''-terthiophene-3' (p-benzoic acid) (TBA) nanocomposite film...
September 20, 2017: Biosensors & Bioelectronics
Sathish Sugumaran, Mohd Faizal Jamlos, Mohd Noor Ahmad, Chandar Shekar Bellan, Dominique Schreurs
Early cancer detection and treatment is an emerging and fascinating field of plasmonic nanobiosensor research. It paves to enrich a life without affecting living cells leading to a possible survival of the patient. This review describes a past and future prospect of an integrated research field on nanostructured metamaterials, microwave transmission, surface plasmonic resonance, nanoantennas, and their manifested versatile properties with nano-biosensors towards early cancer detection to preserve human health...
August 24, 2017: Biosensors & Bioelectronics
Yasaman Sadat Borghei, Morteza Hosseini, Mohammad Reza Ganjali
Here we describe a label-free detection strategy for large deletion mutation in breast cancer related gene BRCA1 based on a DNA-silver Nanocluster Fluorescence upon Recognition-Induced Hybridization. The specific hybridization of DNA templated silver nanoclusters fluorescent probe to target DNAs can act as effective templates for enhancement of AgNCs Fluorescence, which can be used to distinguish the deletion of BRCA1 due to different fluorescent intensities. Under the optimal conditions, the fluorescent intensity of the DNA-AgNCs increased with the increasing deletion type within a dynamic range from 1...
August 31, 2017: Methods and Applications in Fluorescence
Yun Wang, H Christopher Fry, Guy E Skinner, Kristin M Schill, Timothy V Duncan
Botulinum neurotoxin (BoNT) is the most potent toxin known. The ingestion of food contaminated with biologically active BoNT causes foodborne botulism, which can lead to respiratory paralysis, coma, and death after ingestion of as little as 70 μg for a 70 kg human. Because of its lethality and challenges associated with current detection methods, there is an urgent need for highly sensitive rapid screening techniques capable of detecting biologically active BoNT. Here, we describe a Förster resonance energy transfer-based nanobiosensor that uses quantum dots (QDs) and two specific quencher-labeled peptide probes to detect and differentiate two biologically active forms of BoNT, serotypes A and B, which were responsible for 80% of human foodborne botulism cases in the U...
September 20, 2017: ACS Applied Materials & Interfaces
Na Lu, Yanli Wen, Gang Liu, Lei Ding, Caixia Zeng, Ali Aldalbahi, Muhammad Naziruddin Khan, Govindasami Periyasami, Mostafizur Rahaman, Abdulaziz Alrohaili, Jiye Shi, Shiping Song, Lihua Wang, Min Zhang
The excellent performance of a biosensor generally depends on the high signal-to-noise ratio, and the superquencher plays a dominant role in fluorescent sensors. Novel nanoquenchers exhibited high quenching efficiency in various fluorescent assays of biological/chemical molecules. Here, we developed a novel nano-biosensor using Fe3O4@C yolk-shell nanoparticles (YSNPs) and studied their quenching effect. We found Fe3O4@C YSNP was a superquencher and exhibited an ultrastrong quenching ability, up to almost 100% quenching efficiency, toward fluorophores...
September 13, 2017: ACS Applied Materials & Interfaces
Anna Meneghello, Stefano Tartaggia, Maria Domenica Alvau, Federico Polo, Giuseppe Toffoli
Therapeutic drug monitoring (TDM) is the clinical practice of measuring pharmaceutical drug concentrations in patients' biofluids at designated intervals to allow a close and timely control of their dosage. This practice allows for rapid medical intervention in case of toxicity-related issues and/or adjustment of dosage to better fit the therapeutic demand. Currently, TDM is performed in centralized laboratories employing instruments, such as immunoassay analyzers and mass spectrometers that can be run only by trained personnel...
July 20, 2017: Current Medicinal Chemistry
Zengtao Bao, Jialin Sun, Xiaoqian Zhao, Zengyao Li, Songkui Cui, Qingyang Meng, Ye Zhang, Tong Wang, Yanfeng Jiang
Sensitive and quantitative detection of tumor markers is highly required in the clinic for cancer diagnosis and consequent treatment. A field-effect transistor-based (FET-based) nanobiosensor emerges with characteristics of being label-free, real-time, having high sensitivity, and providing direct electrical readout for detection of biomarkers. In this paper, a top-down approach is proposed and implemented to fulfill a novel silicon nano-ribbon FET, which acts as biomarker sensor for future clinical application...
2017: International Journal of Nanomedicine
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