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Nanobiosensor

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https://www.readbyqxmd.com/read/28120920/analysis-of-alternative-splicing-events-for-cancer-diagnosis-using-a-multiplexing-nanophotonic-biosensor
#1
César S Huertas, Santos Domínguez-Zotes, Laura M Lechuga
Personalized medicine is a promising tool not only for prevention, screening and development of more efficient treatment strategies, but also for diminishing the side effects caused by current therapies. Deciphering gene regulation pathways provides a reliable prognostic analysis to elucidate the origin of grave diseases and facilitate the selection of the most adequate treatment for each individual. Alternative splicing of mRNA precursors is one of these gene regulation pathways and enables cells to generate different protein outputs from the same gene depending on their developmental or homeostatic status...
January 25, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28107978/fret-based-nanobiosensor-for-detection-of-scopolamine-in-hairy-root-extraction-of-atropa-belladonna
#2
Fereshte Bagheri, Khosro Piri, Afshine Mohsenifar, Smaiil Ghaderi
A simple, sensitive, selective, and rapid optical nanobiosensor based on FRET was designed to detect tropane alkaloids as anti-cholinergic agents in natural and transgenic hairy roots extracts of Atropa belladonna. To achieve that, conjugation of tioglycolyic acid capped cadmium telluride quantum Dots, M2 muscarinic receptor (Cd/Te QDs-M2R) and conjugation of scopolamine-rhodamine123 (Sc-Rho123) were performed. More specifically, proportional amounts of M2 muscarinic receptor and quantum dots (QDs) were conjugated while scopolamine (as a tropane alkaloid) and rhodamine123 were also combined and these moieties functioned as donor and acceptor pairs, respectively...
March 1, 2017: Talanta
https://www.readbyqxmd.com/read/28081500/a-highly-sensitive-quantum-dots-dna-nanobiosensor-based-on-fluorescence-resonance-energy-transfer-for-rapid-detection-of-nanomolar-amounts-of-human-papillomavirus-18
#3
Mojtaba Shamsipur, Vahid Nasirian, Kamran Mansouri, Ali Barati, Asad Veisi-Raygani, Soheila Kashanian
A very sensitive and convenient nanobiosensor based on fluorescence resonance energy transfer (FRET) was developed for the detection of a 22-mer oligonucleotides sequence in Human Papillomavirus 18 virus (HPV18) gene. For this purpose, water-soluble CdTe quantum dots (QDs) were synthesized and, subsequently, amino-modified 11-mer oligonucleotide as one of the two necessary probes was attached to QDs surface to form functional QDs-DNA conjugates. Right after addition of the QDs-DNA and a second Cyanine5 (Cy5)-labeled 11-mer oligonucleotide probe to the DNA target solution, the sandwiched hybrids were formed...
March 20, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28033555/unbinding-pathway-energy-of-glyphosate-from-the-epsps-enzyme-binding-site-characterized-by-steered-molecular-dynamics-and-potential-of-mean-force
#4
Moacir F Ferreira, Eduardo F Franca, Fábio L Leite
The quantification of herbicides in the environment, like glyphosate, is extremely important to prevent contamination. Nanobiosensors stands out in the quantization process, because of the high selectivity, sensitivity and short response time of the method. In order to emulate the detection of glyphosate using a specific nanobiossensor through an Atomic Force Microscope (AFM), this work carried out Steered Molecular Dynamics simulations (SMD) in which the herbicide was unbinded from the active site of the enzyme 5- enolpyruvylshikimate 3 phosphate synthase (EPSPS) along three different directions...
November 21, 2016: Journal of Molecular Graphics & Modelling
https://www.readbyqxmd.com/read/28002949/comprehensive-study-of-the-effects-of-nanopore-structures-on-enzyme-activity-for-the-enzyme-based-electrochemical-biosensors-based-on-molecular-simulation
#5
Guang Yang, Bo Liang, Qin Zhu, Yichuan Hu, Xuesong Ye
Assembly of biocompatible nanostructures to retain the enzyme activity and improve the biocatalytic ability is a decisive factor for enhancing the performance of enzyme biosensors. However, there is still a lack of molecular level understandings of the physicochemical interaction mechanism at the interface of biosensor electrodes and enzymes. Here, for the first time at molecular level, the effects of two classic biosensor electrode materials with different electrical properties and morphologies and glucose oxidase (GOD) on retaining the enzyme conformation were analyzed by molecular dynamics simulation...
December 22, 2016: Journal of Physical Chemistry. A
https://www.readbyqxmd.com/read/27995447/ethics-nanobiosensors-and-elite-sport-the-need-for-a-new-governance-framework
#6
Robert Evans, Michael McNamee, Owen Guy
Individual athletes, coaches and sports teams seek continuously for ways to improve performance and accomplishment in elite competition. New techniques of performance analysis are a crucial part of the drive for athletic perfection. This paper discusses the ethical importance of one aspect of the future potential of performance analysis in sport, combining the field of biomedicine, sports engineering and nanotechnology in the form of 'Nanobiosensors'. This innovative technology has the potential to revolutionise sport, enabling real time biological data to be collected from athletes that can be electronically distributed...
December 19, 2016: Science and Engineering Ethics
https://www.readbyqxmd.com/read/27940406/nanoscale-sensors-for-assuring-the-safety-of-food-products
#7
REVIEW
Yun Wang, Timothy V Duncan
As far as chemical analysis is concerned, foods are among the most difficult matrices to work with because they are complex, heterogeneous substances with a high degree of variety. Assaying foods for trace levels of chemical and microbiological substances is a challenge that often requires the application of time-consuming, expensive analytical instrumentation in dedicated facilities populated by highly trained personnel. Therefore there is a continued demand for new analytical technologies that can detect small concentrations of chemicals or microbes in a more cost- and time-effective manner, preferably in the field, on the production line, and/or non-destructively, with little to no sample pre-treatment, and possibly by individuals with scant scientific training...
December 9, 2016: Current Opinion in Biotechnology
https://www.readbyqxmd.com/read/27940241/electrospun-manganese-iii-oxide-nanofiber-based-electrochemical-dna-nanobiosensor-for-zeptomolar-detection-of-dengue-consensus-primer
#8
Suryasnata Tripathy, Siva Rama Krishna Vanjari, Vikrant Singh, S Swaminathan, Shiv Govind Singh
Nanoscale biosensors, owing to their high-sensitivity and extremely low limits-of-detection, have enabled the realization of highly complex and sophisticated miniaturized platforms for several important healthcare applications, the most predominant one being disease diagnosis. In particular, nanomaterial facilitated electrochemical detection of DNA hybridization has had an exceptional impact on fields such as genetics and cancerous mutation detection Here we report an ultrasensitive electrochemical platform using electrospun semi-conducting Manganese (III) Oxide (Mn2O3) nanofibers for DNA Hybridization detection...
April 15, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/27909844/design-and-fabrication-a-gold-nanoparticle-dna-based-nanobiosensor-for-detection-of-microrna-involved-in-alzheimer-s-disease
#9
Shokoufeh Delkhahi, Mahdi Rahaie, Fereshteh Rahimi
MicroRNAs are small RNAs which regulate gene expression by translational repression or degradation of messenger RNAs. Regards to important role of these biomolecules in human disease progress, to produce sensitive, simple and cost-effective assays for microRNAs are in urgent demand. miR-137 in Alzheimer's patients has demonstrated its potential as non-invasive biomarkers in blood for Alzheimer's disease diagnosis and prognosis. This paper describes a novel, sensitive and specific microRNA assay based on Colorimetric detection of gold nanoparticles and hybridization chain reaction amplification (HCR)...
December 1, 2016: Journal of Fluorescence
https://www.readbyqxmd.com/read/27864741/an-ultrasensitive-label-free-nanobiosensor-platform-for-the-detection-of-cardiac-biomarkers
#10
Durga Prakash Matta, Suryasnata Tripathy, Siva Rama Krishna Vanjari, Chandra Shekhar Sharma, Shiv Govind Singh
We report the fabrication of a label free nano biosensor platform comprising single nanofiber that is derived out of multi-walled carbon nanotubes (MWCNTs) embedded SU-8 photoresist, for the detection of three important human cardiac biomarkers viz., myoglobin (Myo), cardiac Troponin I (cTn I) and Creatine Kinase-MB (CK-MB). These composite nanofibers were synthesized using electrospinning process. Single nanofibers were aligned between pairs of electrodes in-situ during the electrospinning process. The target proteins were detected using chemiresistive detection methodology...
December 2016: Biomedical Microdevices
https://www.readbyqxmd.com/read/27858297/novel-fluorometric-assay-for-detection-of-cysteine-as-a-reducing-agent-and-template-in-formation-of-copper-nanoclusters
#11
Yasaman-Sadat Borghei, Morteza Hosseini, Mehdi Khoobi, Mohammad Reza Ganjali
This study was designed to develop a highly selective and sensitive method towards fluorimetric sensing of cysteine (Cys) in water and human serum by using copper nanocluster. The Cys-CuNCs were characterized by scanning electron microscopy (SEM), FTIR, fluorescence and UV-Vis analysis. Spectroscopic evidences showed different intensities that were attributed to the different size of Cys-CuNCs. Enhancement in fluorescence intensity of copper nanoclusters with an increase in concentration of cysteine may enable them to be good candidates in detection systems...
November 17, 2016: Journal of Fluorescence
https://www.readbyqxmd.com/read/27855935/fret-based-aptamer-biosensor-for-selective-and-sensitive-detection-of-aflatoxin-b1-in-peanut-and-rice
#12
Fereshte Sadat Sabet, Morteza Hosseini, Hossein Khabbaz, Mehdi Dadmehr, Mohammad Reza Ganjali
Aflatoxins are potential food pollutants produced by fungi. Among them, Aflatoxin B1 (AFB1) is the most toxic. Therefore, a great deal of concern is associated with AFB1 toxicity. In this work, utilizing a FRET-based method, we have developed a nanobiosensor for detection of AFB1 in agricultural foods. Aptamer-conjugated Quantum dots (QDs) are adsorbed to Au nanoparticles (AuNPs) due to interaction of aptamers with AuNPs leading to quenching effect on QDs fluorescence. Upon the addition of AFB1, the specific aptamers are attracted to AFB1, getting distance from AuNPs which result in fluorescence recovery...
April 1, 2017: Food Chemistry
https://www.readbyqxmd.com/read/27825520/versatility-of-a-localized-surface-plasmon-resonance-based-gold-nanoparticle-alloyed-quantum-dot-nanobiosensor-for-immunofluorescence-detection-of-viruses
#13
Kenshin Takemura, Oluwasesan Adegoke, Naoto Takahashi, Tatsuya Kato, Tian-Cheng Li, Noritoshi Kitamoto, Tomoyuki Tanaka, Tetsuro Suzuki, Enoch Y Park
Flu infection, caused by the influenza virus, constitutes a serious threat to human lives worldwide. A rapid, sensitive and specific diagnosis is urgently needed for point-of-care treatment and to control the rapid spread of this disease. In this study, an ultrasensitive, rapid and specific localized surface plasmon resonance (LSPR)-induced immunofluorescence nanobiosensor has been developed for the influenza virus based on a gold nanoparticle (AuNP)-induced quantum dot (QD) fluorescence signal. Alloyed quaternary CdSeTeS QDs were synthesized via the hot-injection organometallic route and were subsequently capped with l-cysteine via a ligand exchange reaction...
March 15, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/27822647/development-of-quantum-dot-encapsulated-liposome-based-optical-nanobiosensor-for-detection-of-telomerase-activity-without-target-amplification
#14
Ali Zavari-Nematabad, Mohammadreza Alizadeh-Ghodsi, Hamed Hamishehkar, Esmaeel Alipour, Younes Pilehvar-Soltanahmadi, Nosratollah Zarghami
Reactivation of telomerase, which is observed in more than 85% of all known human tumours, is considered a promising tumour marker for cancer diagnosis. With respect to the biomedical importance of telomerase, we have developed a simple strategy based on liposomal fluorescent signal amplification for highly sensitive optical detection of telomerase activity using liposome-encapsulated cadmium telluride quantum dots. In this strategy, telomerase extracted from A549 cells elongated the biotinylated telomerase substrate primer, which was then immobilized on streptavidine-coated microplate wells...
November 7, 2016: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/27796511/neuromolecular-imaging-a-nanobiotechnology-for-parkinson-s-disease-advancing-pharmacotherapy-for-personalized-medicine
#15
P A Broderick, L Wenning, Y-S Li
Evaluating each patient and animal as its own control achieves personalized medicine, which honors the hippocratic philosophy, explaining that "it is far more important to know what person has the disease than what disease the person has." Similarly, individualizing molecular signaling directly from the patient's brain in real time is essential for providing prompt, patient-based treatment as dictated by the point of care. Fortunately, nanotechnology effectively treats many neurodegenerative diseases. In particular, the new medicinal frontier for the discovery of therapy for Parkinson's disease is nanotechnology and nanobiotechnology...
October 28, 2016: Journal of Neural Transmission
https://www.readbyqxmd.com/read/27717857/localized-surface-plasmon-resonance-based-gold-nanobiosensor-determination-of-thyroid-stimulating-hormone
#16
Afsaneh Salahvarzi, Mohamad Mahani, Masoud Torkzadeh-Mahani, Reza Alizadeh
An immunoassay method based on the peak shift of the localized surface plasmon resonance (LSPR) absorption maxima has been developed for the determination of the thyroid stimulating hormone (TSH) in human blood serum. The anti-TSH antibody was adsorbed on the synthesized gold nanoparticles by electrostatic forces. The efficiency of the nanobiosensor was improved by optimizing the factors affecting the probe construction such as the pH and the antibody to gold nanoparticles ratio. Dynamic light scattering was applied for the characterization of the constructed probe...
October 4, 2016: Analytical Biochemistry
https://www.readbyqxmd.com/read/27614683/a-fluorescence-turn-on-biosensor-based-on-graphene-quantum-dots-gqds-and-molybdenum-disulfide-mos2-nanosheets-for-epithelial-cell-adhesion-molecule-epcam-detection
#17
Jingyu Shi, Jing Lyu, Feng Tian, Mo Yang
This paper presents a "turn-on" fluorescence biosensor based on graphene quantum dots (GQDs) and molybdenum disulfide (MoS2) nanosheets for rapid and sensitive detection of epithelial cell adhesion molecule (EpCAM). PEGylated GQDs were used as donor molecules, which could not only largely increase emission intensity but also prevent non-specific adsorption of PEGylated GQD on MoS2 surface. The sensing platform was realized by adsorption of PEGylated GQD labelled EpCAM aptamer onto MoS2 surface via van der Waals force...
September 4, 2016: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/27563905/ultrasensitive-label-free-chemiresistive-nanobiosensor-using-multiwalled-carbon-nanotubes-embedded-electrospun-su-8-nanofibers
#18
Matta Durga Prakash, Siva Rama Krishna Vanjari, Chandra Shekhar Sharma, Shiv Govind Singh
This paper reports the synthesis and fabrication of aligned electrospun nanofibers derived out of multiwalled carbon nanotubes (MWCNTs) embedded SU-8 photoresist, which are targeted towards ultrasensitive biosensor applications. The ultrasensitivity (detection in the range of fg/mL) and the specificity of these biosensors were achieved by complementing the inherent advantages of MWCNTs such as high surface to volume ratio and excellent electrical and transduction properties with the ease of surface functionalization of SU-8...
2016: Sensors
https://www.readbyqxmd.com/read/27558349/a-nanobiosensor-for-the-detection-of-arginase-activity
#19
Aruni P Malalasekera, Hongwang Wang, Thilani N Samarakoon, Dinusha N Udukala, Asanka S Yapa, Raquel Ortega, Tej B Shrestha, Hamad Alshetaiwi, Emily J McLaurin, Deryl L Troyer, Stefan H Bossmann
A nanobiosensor for arginase detection was designed and synthesized. It features a central dopamine-coated iron/iron oxide nanoparticle to which sulfonated cyanine 7.0 is tethered via a stable amide bond. Cyanine 5.5 is linked to the N-terminal of the peptide sequence GRRRRRRRG. Arginine (R) reacts to ornithine (O) in the presence of arginase. Based on calibration with commercially obtained arginase II, the limit of detection (LOD) is picomolar. It is noteworthy that the nanobiosensor for arginase detection does not show a fluorescence increase when incubated with the enzyme NO-reductase, which also uses arginase as substrate, but is indicative of an inflammatory response by the host to cancer and infections...
August 21, 2016: Nanomedicine: Nanotechnology, Biology, and Medicine
https://www.readbyqxmd.com/read/27547924/a-nanobiosensor-for-dynamic-single-cell-analysis-during-microvascular-self-organization
#20
S Wang, J Sun, D D Zhang, P K Wong
The formation of microvascular networks plays essential roles in regenerative medicine and tissue engineering. Nevertheless, the self-organization mechanisms underlying the dynamic morphogenic process are poorly understood due to a paucity of effective tools for mapping the spatiotemporal dynamics of single cell behaviors. By establishing a single cell nanobiosensor along with live cell imaging, we perform dynamic single cell analysis of the morphology, displacement, and gene expression during microvascular self-organization...
October 14, 2016: Nanoscale
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