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https://www.readbyqxmd.com/read/28225893/lncrna-snhg12-promotes-cell-growth-and-inhibits-cell-apoptosis-in-colorectal-cancer-cells
#1
J Z Wang, C L Xu, H Wu, S J Shen
Several long non-coding RNA (lncRNA) might be correlated with the prognosis of colorectal cancer (CRC) and serve as a diagnostic and prognostic biomarker. However, the exact expression pattern of small nucleolar RNA host gene 12 (SNHG12) in colorectal cancer and its clinical significance remains unclear. The level of SNHG12 was detected by qRT-PCR in CRC tissues and CRC cells. MTT assay and colony formation assay were performed to examine the cell proliferation of CRC cells transfected with pcDNA-SNHG12 or si-SNHG12...
February 20, 2017: Brazilian Journal of Medical and Biological Research, Revista Brasileira de Pesquisas Médicas e Biológicas
https://www.readbyqxmd.com/read/28214867/the-novel-long-noncoding-rna-tusc7-inhibits-proliferation-by-sponging-mir-211-in-colorectal-cancer
#2
Jian Xu, Rui Zhang, Jian Zhao
BACKGROUND/AIMS: The novel long noncoding RNA (lncRNA) tumor suppressor candidate 7 (TUSC7) has been reported as a potential tumor suppressor, while the functional role of TUSC7 is still unknown in colorectal cancer (CRC). Here, we characterized TUSC7 expression profile in CRC patients and investigated its biological function and potential molecular mechanism. METHODS: RNA isolation, qRT-PCR, cell counter kit-8 assay, cell cycle assay, EdU assay, and western blot were performed...
February 8, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28214639/congenital-glioblastoma-with-distinct-clinical-and-molecular-characteristics-case-reports-and-a-literature-review
#3
Masahiro Kameda, Yoshihiro Otani, Tomotsugu Ichikawa, Akira Shimada, Koichi Ichimura, Isao Date
BACKGROUND: The molecular diagnosis of brain tumors is important in classifying tumors and determining appropriate treatment. Congenital glioblastoma multiforme (GBM) is a rare tumor that occurs in infants, and the prognosis is poor. Approximately 60 patients diagnosed as congenital GBM have been reported. However, few reports have conducted molecular analyses of congenital GBM. CASE DESCRIPTION: We describe two congenital GBM patients treated in our hospital, and report results of immunohistochemistry, fluorescent in situ hybridization (FISH), direct sequencing, and methylation analyses...
February 15, 2017: World Neurosurgery
https://www.readbyqxmd.com/read/28193906/cbx3-promotes-colon-cancer-cell-proliferation-by-cdk6-kinase-independent-function-during-cell-cycle
#4
Yao Fan, Haiping Li, Xiaolong Liang, Zheng Xiang
Heterochromatin protein 1γ (CBX3) links histone methylation marks to transcriptional silence, DNA repair and RNA splicing, but a role for CBX3 in cancer remains largely unknown. In this study, we show that CBX3 in colon cancer cells promotes the progression of the cell cycle and proliferation in vitro and in vivo. Cell cycle (G1 phase to S phase) related gene CDK6 and p21 were further identified as targets of CBX3. In addition, we found that enhancing CDK6 suppresses cell proliferation by upregulating inhibitor p21 in the absence of CBX3, and this function is independent of the kinase activity of CDK6...
February 10, 2017: Oncotarget
https://www.readbyqxmd.com/read/28192398/cyclind-cdk4-6-complexes-phosphorylate-cdc25a-and-regulate-its-stability
#5
C Dozier, L Mazzolini, C Cénac, C Froment, O Burlet-Schiltz, A Besson, S Manenti
The phosphatase CDC25A is a key regulator of cell cycle progression by dephosphorylating and activating cyclin-CDK complexes. CDC25A is an unstable protein expressed from G1 until mitosis. CDC25A overexpression, which can be caused by stabilization of the protein, accelerates the G1/S and G2/M transitions, leading to genomic instability and promoting tumorigenesis. Thus, controlling CDC25A protein levels by regulating its stability is a critical mechanism for timing cell cycle progression and to maintain genomic integrity...
February 13, 2017: Oncogene
https://www.readbyqxmd.com/read/28171795/multifunctional-silver-nanocluster-hybrid-oligonucleotide-vehicle-for-cell-imaging-and-microrna-targeted-gene-silencing
#6
Hau-Yun Chen, Karunya Albert, Cheng-Che Wen, Pei-Ying Hsieh, Sih-Yu Chen, Nei-Chung Huang, Shen-Chuan Lo, Jen-Kun Chen, Hsin-Yun Hsu
Novel therapeutics is urgently needed to prevent cancer-related deaths. MicroRNAs that act as tumor suppressors have been recognized as a next-generation tumor therapy, and the restoration of tumor-suppressive microRNAs using microRNA replacements or mimics may be a less toxic, more effective strategy due to fewer off-target effects. Here, we designed the novel multifunctional oligonucleotide nanocarrier complex composed of a tumor-targeting aptamer sequence specific to mucin 1 (MUC1), poly-cytosine region for fluorescent silver nanocluster (AgNC) synthesis, and complimentary sequence for microRNA miR-34a loading...
January 30, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28168755/palbociclib-can-overcome-mutations-in-cyclin-dependent-kinase-6-that-break-hydrogen-bonds-between-the-drug-and-the-protein
#7
Stella Hernandez Maganhi, Patrizia Jensen, Ignez Caracelli, Julio Zukerman Schpector, Stefan Fröhling, Ran Friedman
Inhibition of cyclin dependent kinases (CDKs) 4 and 6 prevent cells from entering the synthesis phase of the cell cycle. CDK4 and 6 are therefore important drug targets in various cancers. The selective CDK4/6 inhibitor palbociclib is approved for the treatment of breast cancer and has shown activity in a cellular model of mixed lineage leukaemia (MLL)-rearranged acute myeloid leukaemia (AML). We studied the interactions of palbociclib and CDK6 using molecular dynamics simulations. Analysis of the simulations suggested several interactions that stabilised the drug in its binding site and that were not observed in the crystal structure of the protein-drug complex...
February 7, 2017: Protein Science: a Publication of the Protein Society
https://www.readbyqxmd.com/read/28156111/highly-potent-selective-and-orally-bioavailable-4-thiazol-n-pyridin-2-yl-pyrimidin-2-amine-cyclin-dependent-kinases-4-and-6-inhibitors-as-anticancer-drug-candidates-design-synthesis-and-evaluation
#8
Solomon Tadesse, Mingfeng Yu, Laychiluh B Mekonnen, Frankie Lam, Saiful Islam, Khamis Tomusange, Muhammed H Rahaman, Benjamin Noll, Sunita K C Basnet, Theodosia Teo, Hugo Albrecht, Robert Milne, Shudong Wang
Cyclin D dependent kinases (CDK4 and CDK6) regulate entry into S phase of the cell cycle and are validated targets for anticancer drug discovery. Herein we detail the discovery of a novel series of 4-thiazol-N-(pyridin-2-yl)pyrimidin-2-amine derivatives as highly potent and selective inhibitors of CDK4 and CDK6. Medicinal chemistry optimization resulted in 83, an orally bioavailable inhibitor molecule with remarkable selectivity. Repeated oral administration of 83 caused marked inhibition of tumor growth in MV4-11 acute myeloid leukemia mouse xenografts without having a negative effect on body weight and showing any sign of clinical toxicity...
February 16, 2017: Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/28151717/synergistic-drug-combinations-with-a-cdk4-6-inhibitor-in-t-cell-acute-lymphoblastic-leukemia
#9
Yana Pikman, Gabriela Alexe, Giovanni Roti, Amy Saur Conway, Andrew Furman, Emily S Lee, Andrew E Place, Sunkyu Kim, Chitra Saran, Rebecca Modiste, David M Weinstock, Marian Harris, Andrew L Kung, Lewis B Silverman, Kimberly Stegmaier
PURPOSE: While significant progress has been made in the treatment of T-cell acute lymphoblastic leukemia (T-ALL), many patients will require additional therapy for relapsed/refractory disease. Cyclin D3 (CCND3) and CDK6 are highly expressed in T-ALL and have been effectively targeted in mutant NOTCH1-driven mouse models of this disease with a CDK4/6 small-molecule inhibitor. Combination therapy, however, will be needed for the successful treatment of human disease. EXPERIMENTAL DESIGN: We performed preclinical drug testing using a panel of T-ALL cell lines first with LEE011, a CDK4/6 inhibitor, and next with the combination of LEE011 with a panel of drugs relevant to T-ALL treatment...
November 9, 2016: Clinical Cancer Research: An Official Journal of the American Association for Cancer Research
https://www.readbyqxmd.com/read/28130125/histone-methyltransferase-setd2-is-critical-for-the-proliferation-and-differentiation-of-myoblasts
#10
Xin Yi, Ye Tao, Xi Lin, Yuan Dai, Tingli Yang, Xiaojing Yue, Xuejun Jiang, Xiaoyan Li, Ding-Sheng Jiang, Kelsey C Andrade, Jiang Chang
Skeletal muscle cell proliferation and differentiation are tightly regulated. Epigenetic regulation is a major component of the regulatory mechanism governing these processes. Histone modification is part of the epigenetic code used for transcriptional regulation of chromatin through the establishment of an active or repressive state for genes involved in myogenesis in a temporal manner. Here, we uncovered the function of SET domain containing 2 (Setd2), an essential histone 3 lysine 36 trimethyltransferase, in regulating the proliferation and differentiation of myoblasts...
January 24, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28129112/therapeutic-mir-21-silencing-ameliorates-diabetic-kidney-disease-in-mice
#11
Malte Kölling, Tamas Kaucsar, Celina Schauerte, Anika Hübner, Angela Dettling, Joon-Keun Park, Martin Busch, Xaver Wulff, Matthias Meier, Kristian Scherf, Nóra Bukosza, Gábor Szénási, Mária Godó, Amit Sharma, Michael Heuser, Peter Hamar, Claudia Bang, Hermann Haller, Thomas Thum, Johan M Lorenzen
Diabetic nephropathy is the main cause of end-stage renal disease. MicroRNAs are powerful regulators of the genome, and global expression profiling revealed miR-21 to be among the most highly regulated microRNAs in kidneys of mice with diabetic nephropathy. In kidney biopsies of diabetic patients, miR-21 correlated with tubulointerstitial injury. In situ PCR analysis showed a specific enrichment of miR-21 in glomerular cells. We identified cell division cycle 25a (Cdc25a) and cyclin-dependent kinase 6 (Cdk6) as novel miR-21 targets in mesangial cells...
January 4, 2017: Molecular Therapy: the Journal of the American Society of Gene Therapy
https://www.readbyqxmd.com/read/28127702/effect-of-sodium-butyrate-on-cell-proliferation-and-cell-cycle-in-porcine-intestinal-epithelial-ipec-j2-cells
#12
Yueqin Qiu, Xianyong Ma, Xuefen Yang, Li Wang, Zongyong Jiang
Conflicting results have been reported that butyrate in normal piglets leads either to an increase or to a decrease of jejunal villus length, implying a possible effect on the proliferation of enterocytes. No definitive study was found for the biological effects of butyrate in porcine jejunal epithelial cells. The present study used IPEC-J2 cells, a non-transformed jejunal epithelial line to evaluate the direct effects of sodium butyrate on cell proliferation, cell cycle regulation, and apoptosis. Low concentrations (0...
January 27, 2017: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/28127258/tnf-%C3%AE-sensitizes-chemotherapy-and-radiotherapy-against-breast-cancer-cells
#13
Xiao Wu, Meng-Yao Wu, Min Jiang, Qiaoming Zhi, Xiaojie Bian, Meng-Dan Xu, Fei-Ran Gong, Juan Hou, Min Tao, Liu-Mei Shou, Weiming Duan, Kai Chen, Meng Shen, Wei Li
PURPOSE: Despite new developments in cancer therapy, chemotherapy and radiotherapy remain the cornerstone of breast cancer treatment. Therefore, finding ways to reduce the toxicity and increase sensitivity is particularly important. Tumor necrosis factor alpha (TNF-α) exerts multiple functions in cell proliferation, differentiation and apoptosis. In the present study, we investigated whether TNF-α could enhance the effect of chemotherapy and radiotherapy against breast cancer cells...
2017: Cancer Cell International
https://www.readbyqxmd.com/read/28114995/prognostic-and-therapeutic-value-of-disruptor-of-telomeric-silencing-1-like-dot1l-expression-in-patients-with-ovarian-cancer
#14
Xiaoxue Zhang, Dan Liu, Mengchen Li, Canhui Cao, Dongyi Wan, Bixin Xi, Wenqian Li, Jiahong Tan, Ji Wang, Zhongcai Wu, Ding Ma, Qinglei Gao
BACKGROUND: Epigenetics has been known to play a critical role in regulating the malignant phenotype. This study was designed to examine the expression of DOT1L (histone 3 lysine 79 methyltransferase) and H3K79 methylation in normal ovarian tissues and ovarian tumors and to explore the function of DOT1L and its underline mechanisms in ovarian cancer. METHODS: The expression of DOT1L and H3K79 methylation in 250 ovarian tumor samples and 24 normal ovarian samples was assessed by immunohistochemistry...
January 23, 2017: Journal of Hematology & Oncology
https://www.readbyqxmd.com/read/28112369/hist1h3d-a-promising-therapeutic-target-for-lung-cancer
#15
Yan Rui, Wen-Jia Peng, Ming Wang, Qian Wang, Zi-Li Liu, Yu-Qing Chen, Li-Nian Huang
HIST1H3D gene encodes histone H3.1 and is involved in gene-silencing and heterochromatin formation. HIST1H3D expression is upregulated in primary gastric cancer tissue. In this study, we explored the effects of HIST1H3D expression on lung cancer, and its mechanisms. HIST1H3D expression was measured by immunohistochemistry and RT-PCR in lung cancer tissues and human lung cancer cell lines. Cell proliferation was assessed by MTT assay. Flow cytometric analysis was used to determine cell cycle distribution and apoptosis...
March 2017: International Journal of Oncology
https://www.readbyqxmd.com/read/28105727/placental-dysfunction-is-associated-with-altered-microrna-expression-in-pregnant-women-with-low-folate-status
#16
Bernadette C Baker, Fiona L Mackie, Samantha C Lean, Susan L Greenwood, Alexander Ep Heazell, Karen Forbes, Rebecca L Jones
SCOPE: Low maternal folate status during pregnancy increases the risk of delivering small for gestational age (SGA) infants, but the mechanistic link between maternal folate status, SGA and placental dysfunction is unknown. microRNAs (miRNA) are altered in pregnancy pathologies and by folate in other systems. We hypothesized low maternal folate status causes placental dysfunction, mediated by altered miRNA expression. METHODS AND RESULTS: A prospective observational study recruited pregnant adolescents, and assessed third trimester folate status and placental function...
January 20, 2017: Molecular Nutrition & Food Research
https://www.readbyqxmd.com/read/28105209/upregulated-microrna-143-inhibits-cell-proliferation-in-human-nasopharyngeal-carcinoma
#17
Benfu He, Zhe Xu, Jinzhang Chen, Dayong Zheng, Aimin Li, Luo-Sheng Zhang
The aim of the present study was to investigate the possible functions and mechanism of microRNA (miR)-143 in cell proliferation of human nasopharyngeal carcinoma (NPC). The expression of miR-143 in NPC cells and tissues was investigated using reverse transcription-quantitative polymerase chain reaction. Cell viability assay, colony formation assay and flow cytometry were used to examine the cell proliferative ability and tumorigenicity. The expression levels of p21(Cip1), p27(Kip1), cyclin D1, phosphorylated (p)-retinoblastoma protein (Rb), Rb and cyclin-dependent kinase (CDK) 6 were determined by western blotting...
December 2016: Oncology Letters
https://www.readbyqxmd.com/read/28101580/crude-extract-and-solvent-fractions-of-calystegia-soldanella-induce-g1-and-s%C3%A2-phase-arrest-of-the-cell-cycle-in-hepg2-cells
#18
Jung Im Lee, In-Hye Kim, Taek-Jeong Nam
The representative halophyte Calystegia soldanella (L) Roem. et Schult is a perennial vine herb that grows in coastal dunes throughout South Korea as well as in other regions around the world. This plant has long been used as an edible and medicinal herb to cure rheumatic arthritis, sore throat, dropsy, and scurvy. Some studies have also shown that this plant species exhibits various biological activities. However, there are few studies on cytotoxicity induced by C. soldanella treatment in HepG2 human hepatocellular carcinoma cells...
February 2017: International Journal of Oncology
https://www.readbyqxmd.com/read/28098882/downregulation-of-microrna%C3%A2-34b-is-responsible-for-the-elevation-of-blood-pressure-in-spontaneously-hypertensive-rats
#19
Fan Yang, Haiyu Li, Youyou Du, Qiangwei Shi, Luosha Zhao
The present study aimed to identify the microRNA (miRNA) responsible for the development of primary hypertension, and examine the downstream signaling pathway, which mediates the effect of the miRNA. Reverse transcription‑quantitative polymerase chain reaction analysis was performed to identify which miRNA may be involved in the pathogenesis of hypertension. In silico analysis and a luciferase assay were used to validate the target of the selected miRNA, and miRNA mimics and small interfering (si)RNA of the target were transfected into smooth muscle cells to examine its effect on the biological activity of the cells...
January 16, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28089693/effects-of-six-priority-controlled-phthalate-esters-with-long-term-low-dose-integrated-exposure-on-male-reproductive-toxicity-in-rats
#20
Hai-Tao Gao, Run Xu, Wei-Xin Cao, Liang-Liang Qian, Min Wang, Lingeng Lu, Qian Xu, Shu-Qin Yu
Human beings are inevitably exposed to ubiquitous phthalate esters (PEs) surroundings. The purposes of this study were to investigate the effects of long-term low-dose exposure to the mixture of six priority controlled phthalate esters (MIXPs): dimethyl phthalate (DMP), diethyl phthalate (DEP), di(n-butyl) phthalate (DBP), butyl benzyl phthalate (BBP), di(2-ethyhexyl) phthalate (DEHP) and di-n-octyl phthalate (DNOP), on male rat reproductive system and further to explore the underlying mechanisms of the reproductive toxicity...
January 12, 2017: Food and Chemical Toxicology
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