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time resolution mbs

David Ries, Daniela Holtgräwe, Prisca Viehöver, Bernd Weisshaar
BACKGROUND: The combination of bulk segregant analysis (BSA) and next generation sequencing (NGS), also known as mapping by sequencing (MBS), has been shown to significantly accelerate the identification of causal mutations for species with a reference genome sequence. The usual approach is to cross homozygous parents that differ for the monogenic trait to address, to perform deep sequencing of DNA from F2 plants pooled according to their phenotype, and subsequently to analyze the allele frequency distribution based on a marker table for the parents studied...
2016: BMC Genomics
Jordan Virbalas, Caitlin McMullen, Jeffrey Cheng
OBJECTIVE: Children with cystic fibrosis (CF) are uniquely vulnerable to the pulmonary complications of chronic aspiration. We present a case series of children with CF and evidence of chronic aspiration who underwent injection laryngoplasty to improve the safety and efficacy of their swallow. STUDY DESIGN: Retrospective chart review. SETTING: Tertiary care children's hospital. METHODS/SUBJECTS: A chart review was performed on three consecutive cases of children with CF and evidence of aspiration on modified barium swallow (MBS) evaluated at a tertiary care, academic children's medical center...
November 2015: International Journal of Pediatric Otorhinolaryngology
Giselle A Suero-Abreu, G Praveen Raju, Orlando Aristizábal, Eugenia Volkova, Alexandre Wojcinski, Edward J Houston, Diane Pham, Kamila U Szulc, Daniel Colon, Alexandra L Joyner, Daniel H Turnbull
Mouse models have increased our understanding of the pathogenesis of medulloblastoma (MB), the most common malignant pediatric brain tumor that often forms in the cerebellum. A major goal of ongoing research is to better understand the early stages of tumorigenesis and to establish the genetic and environmental changes that underlie MB initiation and growth. However, studies of MB progression in mouse models are difficult due to the heterogeneity of tumor onset times and growth patterns and the lack of clinical symptoms at early stages...
December 2014: Neoplasia: An International Journal for Oncology Research
Tendy Chiang, Brook McConnell, Amanda G Ruiz, Emily M DeBoer, Jeremy D Prager
INTRODUCTION: Laryngeal clefts (LC) are congenital malformations of the upper aerodigestive tract that can cause dysphagia, aspiration, and subsequent pulmonary complications. This investigation assessed the outcomes of multidisciplinary evaluation and surgical management of type I and II LC (LC1-2) at a tertiary children's hospital. MATERIALS AND METHODS: A retrospective chart review was used to identify patients who underwent repair of LC1-2 by a single surgeon...
December 2014: International Journal of Pediatric Otorhinolaryngology
Ching-Hsiang Fan, Wun-Hao Lin, Chien-Yu Ting, Wen-Yen Chai, Tzu-Chen Yen, Hao-Li Liu, Chih-Kuang Yeh
The blood-brain barrier (BBB) can be transiently and locally opened by focused ultrasound (FUS) in the presence of microbubbles (MBs). Various imaging modalities and contrast agents have been used to monitor this process. Unfortunately, direct ultrasound imaging of BBB opening with MBs as contrast agent is not feasible, due to the inability of MBs to penetrate brain parenchyma. However, FUS-induced BBB opening is accompanied by changes in blood flow and perfusion, suggesting the possibility of perfusion-based ultrasound imaging...
2014: Theranostics
Jean-Marc Hyvelin, Isabelle Tardy, Thierry Bettinger, Mathew von Wronski, Maria Costa, Patricia Emmel, Delphine Colevret, Philippe Bussat, Anne Lassus, Catherine Botteron, Adrian Nunn, Peter Frinking, François Tranquart
OBJECTIVE: The diagnosis of acute coronary syndrome remains challenging especially in patients without clear symptoms or electrocardiographic and/or biomarker features. A hallmark of ischemia/reperfusion is activation of endothelial cells leading to altered expression of molecular markers, including selectins. In this context, we aimed to validate the value of ultrasound molecular imaging for detecting transient myocardial ischemia by using a clinically translatable dual P- and E-selectin-targeted ultrasound contrast agent (UCA) and microbubble (MB(selectin))...
April 2014: Investigative Radiology
Xiaowen Hu, Charles F Caskey, Lisa M Mahakian, Dustin E Kruse, Julie R Beegle, Anne-Emilie Declèves, Joshua J Rychak, Patrick L Sutcliffe, Kumar Sharma, Katherine W Ferrara
Ultrasound can selectively and specifically visualize upregulated vascular receptors through the detection of bound microbubbles. However, most current ultrasound molecular imaging methods incur delays that result in longer acquisition times and reduced frame rates. These delays occur for two main reasons: 1) multi-pulse imaging techniques are used to differentiate microbubbles from tissue and 2) acquisition occurs after free bubble clearance (>6 minutes) in order to differentiate bound from freely circulating microbubbles...
2013: American Journal of Nuclear Medicine and Molecular Imaging
Qi Wang, Lei Chen, Yitao Long, He Tian, Junchen Wu
Molecular beacons (MBs) of DNA and RNA have aroused increasing interest because they allow a continuous readout, excellent spatial and temporal resolution to observe in real time. This kind of dual-labeled oligonucleotide probes can differentiate between bound and unbound DNA/RNA in homogenous hybridization with a high signal-to-background ratio in living cells. This review briefly summarizes the different unnatural sugar backbones of oligonucleotides combined with fluorophores that have been employed to sense DNA/RNA...
2013: Theranostics
Qingzhong Ren, Hao Li, Yanying Wu, Jing Ren, Aike Guo
Inflexible cognition and behavior are prominent features of prefrontal cortex damage and several neuropsychiatric disorders. The ability to flexibly adapt cognitive processing and behavior to dynamically changing environmental contingencies has been studied using the reversal learning paradigm in mammals, but the complexity of the brain circuits precludes a detailed analysis of the underlying neural mechanism. Here we study the neural circuitry mechanism supporting flexible behavior in a genetically tractable model organism, Drosophila melanogaster...
August 22, 2012: Journal of Neuroscience: the Official Journal of the Society for Neuroscience
Xiaowei Wang, Christoph E Hagemeyer, Jan David Hohmann, Ephraem Leitner, Paul C Armstrong, Fu Jia, Manfred Olschewski, Andrew Needles, Karlheinz Peter, Ingo Ahrens
BACKGROUND: Molecular imaging is a fast emerging technology allowing noninvasive detection of vascular pathologies. However, imaging modalities offering high resolution currently do not allow real-time imaging. We hypothesized that contrast-enhanced ultrasound with microbubbles (MBs) selectively targeted to activated platelets would offer high-resolution, real-time molecular imaging of evolving and dissolving arterial thrombi. METHODS AND RESULTS: Lipid-shell based gas-filled MBs were conjugated to either a single-chain antibody specific for activated glycoprotein IIb/IIIa via binding to a Ligand-Induced Binding Site (LIBS-MBs) or a nonspecific single-chain antibody (control MBs)...
June 26, 2012: Circulation
Yossi Kam, Abraham Rubinstein, Aviram Nissan, David Halle, Eylon Yavin
Detection of mRNA alterations is a promising approach for identifying biomarkers as means of differentiating benign from malignant lesions. By choosing the KRAS oncogene as a target gene, two types of molecular beacons (MBs) based on either phosphothioated DNA (PS-DNA-MB) or peptide nucleic acid (TO-PNA-MB, where TO = thiazole orange) were synthesized and compared in vitro and in vivo. Their specificity was examined in wild-type KRAS (HT29) or codon 12 point mutation (Panc-1, SW480) cells. Incubation of both beacons with total RNA extracted from the Panc-1 cell line (fully complementary sequence) showed a fluorescent signal for both beacons...
March 5, 2012: Molecular Pharmaceutics
Amber L Wells, John S Condeelis, Robert H Singer, Daniel Zenklusen
INTRODUCTIONThe MS2 system provides optimal sensitivity for single-molecule detection in cells. It requires two genetically encoded moieties: a reporter mRNA that contains MS2 binding site (MBS) stem loops and a fluorescent MS2 coat protein (MCP-xFP) that binds to the stem loops with high affinity, thus tagging the mRNA within the cell. This protocol describes transfection of COS-7 cells with reporter RNA (e.g., pRSV-Z-24 MBS-β-actin) and MCP-xFP (e.g., pPolII-MCP-GFP-NLS) plasmids using calcium phosphate precipitation...
2007: CSH Protocols
Timothée Lionnet, Kevin Czaplinski, Xavier Darzacq, Yaron Shav-Tal, Amber L Wells, Jeffrey A Chao, Hye Yoon Park, Valeria de Turris, Melissa Lopez-Jones, Robert H Singer
Live-cell single mRNA imaging is a powerful tool but has been restricted in higher eukaryotes to artificial cell lines and reporter genes. We describe an approach that enables live-cell imaging of single endogenous labeled mRNA molecules transcribed in primary mammalian cells and tissue. We generated a knock-in mouse line with an MS2 binding site (MBS) cassette targeted to the 3' untranslated region of the essential β-actin gene. As β-actin-MBS was ubiquitously expressed, we could uniquely address endogenous mRNA regulation in any tissue or cell type...
February 2011: Nature Methods
Carsten Jobelius, Bettina Ruth, Christian Griebler, Rainer U Meckenstock, Juliane Hollender, Anne Reineke, Fritz H Frimmel, Christian Zwiener
Anaerobic degradation processes play an important role in contaminated aquifers. To indicate active biodegradation processes signature metabolites can be used. In this study field samples from a high-resolution multilevel well in a tar oil-contaminated, anoxic aquifer were analyzed for metabolites by liquid chromatography-tandem mass spectrometry and time-of-flight mass spectrometry. In addition to already known specific degradation products of toluene, xylenes, and naphthalenes, the seldom reported degradation products benzothiophenemethylsuccinic acid (BTMS), benzofuranmethylsuccinic acid (BFMS), methylnaphthyl-2-methylsuccinic acid (MNMS), and acenaphthene-5-carboxylic acid (AC) could be identified (BFMS, AC) and tentatively identified (BTMS, MNMS)...
January 15, 2011: Environmental Science & Technology
Ronald Walker, Bart Barlogie, Jeffrey Haessler, Guido Tricot, Elias Anaissie, John D Shaughnessy, Joshua Epstein, Rudy van Hemert, Eren Erdem, Antje Hoering, John Crowley, Ernest Ferris, Klaus Hollmig, Frits van Rhee, Maurizio Zangari, Mauricio Pineda-Roman, Abid Mohiuddin, Shmuel Yaccoby, Jeffrey Sawyer, Edgardo J Angtuaco
PURPOSE: Magnetic resonance imaging (MRI) permits the detection of diffuse and focal bone marrow infiltration in the absence of osteopenia or focal osteolysis on standard metastatic bone surveys (MBSs). PATIENTS AND METHODS: Both baseline MBS and MRI were available in 611 of 668 myeloma patients who were treated uniformly with a tandem autologous transplantation-based protocol and were evaluated to determine their respective merits for disease staging, response assessment, and outcome prediction...
March 20, 2007: Journal of Clinical Oncology: Official Journal of the American Society of Clinical Oncology
K Tolep, C L Getch, G J Criner
Several studies have suggested that swallowing dysfunction and pulmonary aspiration occur in patients receiving prolonged ventilation. However, the incidence of swallowing dysfunction, its rate of resolution, and the sensitivity of tests used to characterize swallowing abnormalities are not well defined. The goals of our study were to evaluate swallowing function in this group of patients by (1) defining the specific swallowing abnormalities that occur in this patient population, (2) comparing the sensitivity of bedside evaluations to modified barium swallow with videofluoroscopy (MBS/VF), (3) performing endoscopic evaluation of the upper airway to characterize glottic function during swallowing, (4) evaluating the relationship between swallowing dysfunction and neuromuscular disorders, and (5) studying the temporal resolution of swallowing abnormalities...
January 1996: Chest
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