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LAMP PCR

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https://www.readbyqxmd.com/read/29154392/rapid-and-simple-detection-of-ureaplasma-species-from-vaginal-swab-samples-using-a-loop-mediated-isothermal-amplification-method
#1
Kazumasa Fuwa, Mitsuko Seki, Yoshiyasu Hirata, Itaru Yanagihara, Yukiko Nakura, Chika Takano, Kazumichi Kuroda, Satoshi Hayakawa
PROBLEM: Ureaplasma species occasionally cause chorioamnionitis and premature labor. We developed a novel assay employing a loop-mediated isothermal amplification (LAMP) method to detect Ureaplasma parvum and Ureaplasma urealyticum. METHOD OF STUDY: Loop-mediated isothermal amplification primers were designed to amplify Ureaplasma-specific ureaseB genes. Four U. parvum strains, 5 U. urealyticum strains and 14 reference bacterial species were evaluated. Forty-six vaginal swab samples were analyzed by LAMP, culture, and PCR...
November 20, 2017: American Journal of Reproductive Immunology: AJRI
https://www.readbyqxmd.com/read/29127650/-plasmodium-ovale-wallikeri-and-plasmodium-ovale-curtisi-malaria-in-senegal-in-2016
#2
M A Diallo, K Diongue, G Diagne, M C Seck, M Ndiaye, B Dièye, Y D Ndiaye, A S Badiane, D Ndiaye
Recently in Senegal, a case of Plasmodium ovale malaria had led to a diagnostic difficulty due to the ignorance of this parasite and the neglect of it. The objective of this study was to actively investigate cases of P. ovale malaria that would be misdiagnosed in the health centre structures of Senegal. The study was conducted in three areas that reflect different epidemiological strata of malaria. Microscopy was performed by microscopy experts on suspected malaria patients. The results were validated by Rougemont real-time PCR...
November 10, 2017: Bulletin de la Société de Pathologie Exotique
https://www.readbyqxmd.com/read/29124021/allele-specific-lamp-gold-nanoparticle-for-characterization-of-single-nucleotide-polymorphisms
#3
Fábio Ferreira Carlos, Bruno Veigas, Ana S Matias, Gonçalo Doria, Orfeu Flores, Pedro V Baptista
Due to their relevance as disease biomarkers and for diagnostics, screening of single nucleotide polymorphism (SNPs) requires simple and straightforward strategies capable to provide results in medium throughput settings. Suitable approaches relying on isothermal amplification techniques have been evolving to substitute the cumbersome and highly specialized PCR amplification detection schemes. Nonetheless, identification of an individual's genotype still requires sophisticated equipment and laborious methods...
December 2017: Biotechnology Reports
https://www.readbyqxmd.com/read/29121500/validation-of-a-salmonella-loop-mediated-isothermal-amplification-assay-in-animal-food
#4
Kelly J Domesle, Qianru Yang, Thomas S Hammack, Beilei Ge
Loop-mediated isothermal amplification (LAMP) has emerged as a promising alternative to PCR for pathogen detection in food testing and clinical diagnostics. This study aimed to validate a Salmonella LAMP method run on both turbidimetry (LAMP I) and fluorescence (LAMP II) platforms in representative animal food commodities. The U.S. Food and Drug Administration (FDA)'s culture-based Bacteriological Analytical Manual (BAM) method was used as the reference method and a real-time quantitative PCR (qPCR) assay was also performed...
October 18, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/29119360/optimization-of-a-magnetic-capture-rt-lamp-assay-for-fast-and-real-time-detection-of-potato-virus-y-and-differentiation-of-n-and-o-serotypes
#5
Krzysztof Treder, Joanna Chołuj, Bogumiła Zacharzewska, Lavanya Babujee, Mateusz Mielczarek, Adam Burzyński, Aurélie M Rakotondrafara
Potato virus Y (PVY) infection has been a global challenge for potato production and the leading cause of downgrading and rejection of seed crops for certification. Accurate and timely diagnosis is a key for effective disease control. Here, we have optimized a reverse transcription loop-mediated amplification (RT-LAMP) assay to differentiate the PVY O and N serotypes. The RT-LAMP assay is based on isothermal autocyclic strand displacement during DNA synthesis. The high specificity of this method relies heavily on the primer sets designed for the amplification of the targeted regions...
November 8, 2017: Archives of Virology
https://www.readbyqxmd.com/read/29091348/validation-of-loop-mediated-isothermal-amplification-lamp-for-fast-and-portable-sex-determination-across-the-phylogeny-of-birds
#6
A Centeno-Cuadros, J L Tella, M Delibes, P Edelaar, M Carrete
PCR is a universal tool for the multiplication of specific DNA sequences. For example, PCR-based sex determination is widely used, and a diversity of primer sets is available. However, this protocol requires thermal cycling and electrophoresis so results are typically obtained in laboratories and several days after sampling. Loop-mediated isothermal amplification (LAMP) is an alternative to PCR that can take molecular ecology outside the laboratory. Although its application has been successfully probed for sex determination in three species of a single avian Family (Raptors, Accipitridae), its generality remains untested and suitable primers across taxa are lacking...
November 1, 2017: Molecular Ecology Resources
https://www.readbyqxmd.com/read/29064403/characterization-of-three-ocular-clinical-isolates-of-p-aeruginosa-viability-biofilm-formation-adherence-infectivity-and-effects-of-glycyrrhizin
#7
Xudong Peng, Sandamali A Ekanayaka, Sharon A McClellan, Ronald P Barrett, Kerry Vistisen, Linda D Hazlett
We selectively characterized three isolates from Pseudomonas aeruginosa keratitis patients and how glycyrrhizin (GLY) affected them. Type III toxins were determined using polymerase chain reaction (PCR). Minimum Inhibitory Concentration (MIC) of GLY and assays for its effects on: time kill, bacterial permeability, and biofilm/adhesion were done. In vivo, C57BL/6 (B6) mice were treated topically with GLY after G81007 infection. Clinical score, photography with a slit lamp and RT-PCR were used to assess treatment effects...
October 24, 2017: Pathogens
https://www.readbyqxmd.com/read/29061160/molecular-prevalence-of-trypanosome-infections-in-cattle-and-tsetse-flies-in-the-maasai-steppe-northern-tanzania
#8
Mary Simwango, Anibariki Ngonyoka, Happiness J Nnko, Linda P Salekwa, Moses Ole-Neselle, Sharadhuli I Kimera, Paul S Gwakisa
BACKGROUND: African trypanosomosis is a disease of public health and economic importance that poses a major threat to the livelihoods of people living in the Maasai Steppe, where there is a significant interaction between people, livestock and wildlife. The vulnerability of the Maasai people to the disease is enhanced by the interaction of their cattle, which act as vehicles for trypanosomes, and tsetse flies close to wildlife in protected areas. This study was aimed at identification of trypanosome infections circulating in cattle and tsetse flies in order to understand their distribution and prevalence in livestock/wildlife interface areas in the Maasai Steppe...
October 23, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/29057765/development-of-lamp-assays-for-the-molecular-detection-of-taeniid-infection-in-canine-in-tibetan-rural-area
#9
Kai Feng, Wei Li, Zhihong Guo, Hong Duo, Yong Fu, Xiuying Shen, Cheng Tie, Rijie E, Changqin Xiao, Yanhong Luo, Guo Qi, Ma Ni, Qingmei Ma, Wataru Yamazaki, Ayako Yoshida, Yoichiro Horii, Kinpei Yagi, Nariaki Nonaka
For field-identification of taeniid cestodes in canine animals in Tibetan area, loop-mediated isothermal amplification (LAMP) assays for Echinococcus multilocularis, E. shiquicus, Taenia hydatigena, T. multiceps, T. pisiformis and T. crassiceps were developed and evaluated along with the reported assay for E. granulosus. The LAMP assays showed specific reaction with their corresponding target species DNA with the detection limit of 1 to 10 pg. Moreover, the assays for E. granulosus, E. multilocularis, T. hydatigena and T...
October 23, 2017: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/29057336/evaluation-of-the-stability-of-lyophilized-loop-mediated-isothermal-amplification-reagents-for-the-detection-of-coxiella-burnetii
#10
Hua-Wei Chen, Wei-Mei Ching
Coxiella burnetii, the causative pathogen for Q fever, is an obligate intracellular bacterium and designated as a biosafety level 3 agent. Detection and quantification of the bacteria with conventional culturing methods is time-consuming and poses significant health risks. Polymerase chain reaction (PCR)-based assays have been developed for detecting C. burnetii and could provide rapid diagnosis. However, they require specialized equipment, including a cold chain for PCR reagents that maintains their stability during storage and transport...
October 2017: Heliyon
https://www.readbyqxmd.com/read/29051751/evaluation-of-different-pcr-based-assays-and-lamp-method-for-rapid-detection-of-phytophthora-infestans-by-targeting-the-ypt1-gene
#11
Mehran Khan, Benjin Li, Yue Jiang, Qiyong Weng, Qinghe Chen
Late blight, caused by the oomycete Phytophthora infestans, is one of the most devastating diseases affecting potato and tomato worldwide. Early diagnosis of the P. infestans pathogen causing late blight should be the top priority for addressing disease epidemics and management. In this study, we performed a loop-mediated isothermal amplification (LAMP) assay, conventional polymerase chain reaction (PCR), nested PCR, and real-time PCR to verify and compare the sensitivity and specificity of the reaction based on the Ypt1 (Ras-related protein) gene of P...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29050792/comparison-of-molecular-detection-pcr-methods-for-chequa-iflavirus-in-freshwater-crayfish-cherax-quadricarinatus
#12
Kitikarn Sakuna, Jennifer Elliman, Leigh Owens
Chequa iflavirus (+ve sense ssRNA virus) infects redclaw crayfish (Cherax quadricarinatus) and it may cause mortality reaching 20-40% after about three weeks following stress. The sequence of the RNA-dependent RNA polymerase at nucleotide position 8383-9873 was used for developing and comparing PCR-based detection protocols. The reverse transcription, quantitative, polymerase chain reaction (RT-qPCR) was specific against nine Picornavirales and crustacean viruses and its' measurement of uncertainty (0.07-1...
January 2018: Journal of Virological Methods
https://www.readbyqxmd.com/read/29047217/spotted-fever-group-rickettsiae-associated-with-ixodid-ticks-in-wild-environment-in-southern-italy
#13
Donato Antonio Raele, Domenico Galante, Nicola Pugliese, Giovanna La Salandra, Maria Assunta Cafiero
Ixodidae ticks are vectors and reservoirs of several species of rickettsiae, and tick-borne rickettsioses are reported worldwide. This study was aimed to verify the distribution of spotted fever group rickettsiae associated with ticks in a wild environment, the National Park of Gargano, where there is proximity between wild and domestic animals, and which is within an endemic area for rickettsiosis. Ticks were collected from animals or vegetation, morphologically identified and tested by a PCR targeting the 17kDa gene, and by a loop-mediated isothermal amplification (LAMP) targeting ompB gene...
October 18, 2017: MicrobiologyOpen
https://www.readbyqxmd.com/read/29046666/molecular-serotype-specific-identification-of-non-type-b-haemophilus-influenzae-by-loop-mediated-isothermal-amplification
#14
Chika Takano, Mitsuko Seki, Dong Wook Kim, Paul E Kilgore, Kazumasa Fuwa, Koji Takahashi, Toshiaki Inazaki, Satoshi Hayakawa
Over the past four decades, the incidence of meningitis caused by Haemophilus influenzae in children has decreased due to widespread vaccination against H. influenzae type b (Hib). The incidence of invasive diseases due to H. influenzae types not included in the vaccines, however, has increased. At present, there are a limited number of diagnostics available to detect non-type b H. influenzae. To address this issue, we developed a rapid, simple, and cost-effective method for detecting serotypes of H. influenzae...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29045058/loop-mediated-isothermal-amplification-lamp-as-an-alternative-to-pcr-a-rapid-on-site-detection-of-gene-doping
#15
Olivier Salamin, Tiia Kuuranne, Martial Saugy, Nicolas Leuenberger
Innovation in medical research has been diverted at multiple occasions to enhance human performance. The predicted great progress in gene therapy has raised some concerns regarding its misuse in the world of sports (gene doping) for several years now. Even though there is no evidence that gene doping has ever been used in sports, the continuous improvement of gene therapy techniques increases the likelihood of abuse. Therefore, since 2004, efforts have been invested by the anti-doping community and WADA for the development of detection methods...
October 17, 2017: Drug Testing and Analysis
https://www.readbyqxmd.com/read/29044149/development-and-evaluation-of-a-rapid-molecular-diagnostic-test-for-zika-virus-infection-by-reverse-transcription-loop-mediated-isothermal-amplification
#16
Yohei Kurosaki, Danyelly Bruneska Gondim Martins, Mayuko Kimura, Andriu Dos Santos Catena, Maria Amélia Carlos Souto Maior Borba, Sandra da Silva Mattos, Haruka Abe, Rokusuke Yoshikawa, José Luiz de Lima Filho, Jiro Yasuda
The recent outbreak of Zika virus (ZIKV) disease caused an enormous number of infections in Central and South America, and the unusual increase in the number of infants born with microcephaly associated with ZIKV infection aroused global concern. Here, we developed a reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay using a portable device for the detection of ZIKV. The assay specifically detected ZIKV strains of both Asian and African genotypes without cross-reactivity with other arboviruses, including Dengue and Chikungunya viruses...
October 18, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29042629/development-of-a-real-time-fluorescence-loop-mediated-isothermal-amplification-assay-for-rapid-and-quantitative-detection-of-ustilago-maydis
#17
Yanyong Cao, Lifeng Wang, Liping Duan, Jingjing Li, Juan Ma, Shuna Xie, Lei Shi, Huiyong Li
The common smut of corn, caused by Ustilago maydis is a troublesome disease of maize. Early and accurate detection of U. maydis is essential for the disease management. In this study, primer set Pep-2 was selected for LAMP (loop-mediated isothermal amplification) from 12 sets of primers targeting three U. maydis effector genes See1, Pit2 and Pep1 according to primer screening. The optimal concentrations of Bst DNA polymerase and Mg(2+) as well as inner/outer primer ratio of the LAMP reaction system were screened by combining a single factor experiment and an orthogonal design arrangement...
October 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29041927/diagnostic-performance-of-the-loop-mediated-isothermal-amplification-lamp-based-illumigene-%C3%A2-malaria-assay-in-a-non-endemic-region
#18
Anne-Sophie De Koninck, Lieselotte Cnops, Mattias Hofmans, Jan Jacobs, Dorien Van den Bossche, Jan Philippé
BACKGROUND: Light microscopy and antigen-based rapid diagnostic tests are the primary diagnostic tools for detecting malaria, although being labour-intensive and frequently challenged by lack of personnel's experience and low levels of parasite density. The latter being especially important in non-endemic settings. Novel molecular techniques aim to overcome this drawback. The objective of this study was to assess the diagnostic performance of the illumigene malaria assay(®) (Meridian Bioscience) compared to microscopy, RDT and real-time PCR...
October 17, 2017: Malaria Journal
https://www.readbyqxmd.com/read/29040080/detection-of-giardia-intestinalis-in-water-samples-collected-from-natural-water-reservoirs-and-wells-in-northern-and-north-eastern-poland-using-lamp-real-time-pcr-and-nested-pcr
#19
Anna Lass, Beata Szostakowska, Krzysztof Korzeniewski, Panagiotis Karanis
Giardia intestinalis is a protozoan parasite, transmitted to humans and animals by the faecal-oral route, mainly through contaminated water and food. Knowledge about the distribution of this parasite in surface water in Poland is fragmentary and incomplete. Accordingly, 36 environmental water samples taken from surface water reservoirs and wells were collected in Pomerania and Warmia-Masuria provinces, Poland. The 50 L samples were filtered and subsequently analysed with three molecular detection methods: loop-mediated isothermal amplification (LAMP), real-time polymerase chain reaction (real-time PCR) and nested PCR...
October 2017: Journal of Water and Health
https://www.readbyqxmd.com/read/29020042/is-mass-drug-administration-against-lymphatic-filariasis-required-in-urban-settings-the-experience-in-kano-nigeria
#20
Dung D Pam, Dziedzom K de Souza, Susan D'Souza, Millicent Opoku, Safiya Sanda, Ibrahim Nazaradden, Ifeoma N Anagbogu, Chukwu Okoronkwo, Emmanuel Davies, Elisabeth Elhassan, David H Molyneux, Moses J Bockarie, Benjamin G Koudou
BACKGROUND: The Global Programme to Eliminate Lymphatic Filariasis (GPELF), launched in 2000, has the target of eliminating the disease as a public health problem by the year 2020. The strategy adopted is mass drug administration (MDA) to all eligible individuals in endemic communities and the implementation of measures to reduce the morbidity of those suffering from chronic disease. Success has been recorded in many rural endemic communities in which elimination efforts have centered...
October 2017: PLoS Neglected Tropical Diseases
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