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LAMP PCR

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https://www.readbyqxmd.com/read/28218268/rapid-and-sensitive-diagnoses-of-dry-root-rot-pathogen-of-chickpea-rhizoctonia-bataticola-taub-butler-using-loop-mediated-isothermal-amplification-assay
#1
Raju Ghosh, Avijit Tarafdar, Mamta Sharma
Dry root rot (DRR) caused by the fungus Rhizoctonia bataticola (Taub.) Butler, is an emerging disease in chickpea. The disease is often mistaken with other root rots like Fusarium wilt, collar rot and black root rot in chickpea. Therefore, its timely and specific detection is important. Current detection protocols are either based on mycological methods or on protocols involving DNA amplification by polymerase chain reaction (PCR). Here we report the rapid and specific detection of R. bataticola using loop-mediated isothermal amplification (LAMP) assay targeting fungal specific 5...
February 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28211246/dna-amplification-in-the-field-move-over-pcr-here-comes-lamp
#2
Patricia L M Lee
It would not be an exaggeration to say that among molecular technologies, it is PCR (polymerase chain reaction) that underpins the discipline of molecular ecology as we know it today. With PCR, it has been possible to target the amplification of particular fragments of DNA, which can then be analysed in a multitude of ways. The capability of PCR to amplify DNA from a mere handful of copies further means that conservationists and ecologists are able to sample DNA unobtrusively and with minimal disturbance to the environment and the organisms of interest...
March 2017: Molecular Ecology Resources
https://www.readbyqxmd.com/read/28185353/human-vaginal-epithelial-cells-augment-autophagy-marker-genes-in-response-to-candida-albicans-infection
#3
Ankit Shroff, Roicy Sequeira, Kudumula Venkata Rami Reddy
PROBLEM: Autophagy plays an important role in clearance of intracellular pathogens. However, no information is available on its involvement in vaginal infections such as vulvo-vaginal candidiasis (VVC). VVC is intimately associated with the immune status of the human vaginal epithelial cells (VECs). The objective of our study is to decipher if autophagy process is involved during Candida albicans infection of VECs. METHODS OF STUDY: In this study, C. albicans infection system was established using human VEC line (VK2/E6E7)...
February 10, 2017: American Journal of Reproductive Immunology: AJRI
https://www.readbyqxmd.com/read/28177044/comparison-of-lamp-and-pcr-for-molecular-mass-screening-of-sand-flies-for-leishmania-martiniquensis-infection
#4
Saruda Tiwananthagorn, Hirotomo Kato, Ranchana Yeewa, Amontip Muengpan, Raxsina Polseela, Saovanee Leelayoova
BACKGROUND: Leishmaniasis caused by Leishmania martiniquensis infection has been reported in human and domestic animals of Martinique Island, Germany, Switzerland, USA, Myanmar and Thailand. The peculiar clinical features of disseminated cutaneous and visceral forms co-existence render the urgent need of specific diagnostic tool to identify the natural sand fly vectors for effective prevention and control strategies. Loop-mediated isothermal amplification (LAMP) of 18S rRNA gene as well as polymerase chain reaction (PCR) of minicircle kinetoplast DNA gene (PCR-mkDNA) have never been applied to detect L...
February 2017: Memórias do Instituto Oswaldo Cruz
https://www.readbyqxmd.com/read/28167125/pma-lamp-for-rapid-detection-of-escherichia-coli-and-shiga-toxins-from-viable-but-non-culturable-state
#5
Muxia Yan, Ling Xu, Hua Jiang, Zhenwen Zhou, Shishui Zhou, Li Zhang
In exposure to outer pressure, microorganisms are capable of entry into the Viable But Non-Culturable (VBNC) state, and thus survive under various elimination processing. The survival microorganisms may yield negative results on culturing, and cause false negative for this golden standard methodology. In this study, a novel PMA-LAMP assay on the detection of Enterohemorrhage E. coli and shiga toxins has been developed and evaluated, with further application on a number of food borne E. coli strains. LAMP primers were designed on the target of rfbe for Enterohemorrhage E...
February 3, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28166235/development-and-clinical-performance-of-high-throughput-loop-mediated-isothermal-amplification-for-detection-of-malaria
#6
Rushini S Perera, Xavier C Ding, Frank Tully, James Oliver, Nigel Bright, David Bell, Peter L Chiodini, Iveth J Gonzalez, Spencer D Polley
BACKGROUND: Accurate and efficient detection of sub-microscopic malaria infections is crucial for enabling rapid treatment and interruption of transmission. Commercially available malaria LAMP kits have excellent diagnostic performance, though throughput is limited by the need to prepare samples individually. Here, we evaluate the clinical performance of a newly developed high throughput (HTP) sample processing system for use in conjunction with the Eiken malaria LAMP kit. METHODS: The HTP system utilised dried blood spots (DBS) and liquid whole blood (WB), with parallel sample processing of 94 samples per run...
2017: PloS One
https://www.readbyqxmd.com/read/28155195/clinical-evaluation-of-the-isothermal-amplification-assays-for-the-detection-of-four-common-respiratory-viruses-in-children-with-pneumonia
#7
Hangyu Zhou, Mengchuan Zhao, Xinna Li, Dan Zhang, Shuaifeng Zhou, Chen Chen, Zhishan Feng, Xuejun Ma
Respiratory viruses are recognized as serious causes of morbidity and mortality in lower respiratory tract infections in patients. Isothermal amplification assays are increasingly used in their detection because of their rapidity, simplicity and cost-effectiveness, when compared to traditional molecular diagnostic methods. However, systematic assessment of these assays in the clinical settings is rarely reported. MEDLINE (Pubmed) searches were done analysing subject headings and words in the abstract related to isothermal amplification assay and virus...
February 2, 2017: Archives of Virology
https://www.readbyqxmd.com/read/28141595/the-role-of-mincle-in-innate-immune-to-fungal-keratitis
#8
Guiqiu Zhao, Qiang Xu, Jing Lin, Wenjun Chen, Tingting Cui, Liting Hu, Nan Jiang
INTRODUCTION: This study aimed to detect the early expression of macrophage-inducible C-type lectin (Mincle) and investigate its role in the innate immune response of fungal keratitis (FK). METHODOLOGY: Wistar rats were used to make fungal keratitis models. The inflammatory responses and corneal lesions were observed by slit-lamp microscope.RT-PCR, immunohistochemistry, and immunofluorescence were used to detect the expression of Mincle in the rat corneal epithelium...
January 30, 2017: Journal of Infection in Developing Countries
https://www.readbyqxmd.com/read/28140634/development-of-loop-mediated-isothermal-amplification-based-diagnostic-assays-for-detection-of-pasteurella-multocida-and-hemorrhagic-septicemia-associated-p-multocida-serotype-b-2
#9
Ahmed M Moustafa, Mark D Bennett
OBJECTIVE To develop 2 rapid loop-mediated isothermal amplification (LAMP) assays for detection of Pasteurella multocida DNA (Pm-LAMP assay) and P multocida DNA from strains associated with hemorrhagic septicemia (HS) in cattle and buffalo (HS-LAMP assay). SAMPLE Solutions containing 16 P multocida strains and 9 other bacterial species at various concentrations. PROCEDURES Optimal conditions were determined for running the Pm-LAMP and HS-LAMP assays. The assays were then used to detect DNA of the test organisms...
February 2017: American Journal of Veterinary Research
https://www.readbyqxmd.com/read/28131444/detection-of-acanthamoeba-spp-in-water-samples-collected-from-natural-water-reservoirs-sewages-and-pharmaceutical-factory-drains-using-lamp-and-pcr-in-china
#10
Anna Lass, Milena Guerrero, Xiuping Li, Gabriele Karanis, Liqing Ma, Panagiotis Karanis
Various species of amoebas belonging to the genus Acanthamoeba are widely distributed in many parts of the world. Some strains of these protozoans may exist as parasites and pose risks to human health as causative agents of serious human diseases. Currently in China there is a lack of information about the distribution of Acanthamoeba strains in the environment. Accordingly, 261 environmental water samples taken from rivers, sewage, and pharmaceutical factory drains were collected in Qinghai Province, China...
January 25, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28127354/dna-amplification-techniques-for-the-detection-of-toxoplasma-gondii-tissue-cysts-in-meat-producing-animals-a-narrative-review-article
#11
REVIEW
Farooq Riaz, Imran Rashid, Haroon Akbar, Wasim Shehzad, Saher Islam, Amna Arshad Bajwa, Khalid Saeed, Kamran Ashraf
BACKGROUND: Toxoplasma gondii is an intracellular parasite, which infects one-third population of world. Humans and animals acquire infection by ingesting oocytes from feces of cats or by meat of other animals having cysts that may lead to congenital, ocular or cephalic toxoplasmosis. Either it is important to detect T. gondii from meat of food animals from retail shops or directly at slaughterhouses, which is meant for export. METHODS: The current research was done without time limitation using such terms as follows: "Toxoplasma gondii", "Meat", "Tissue cyst", "PCR", "LAMP", "Screening" and "Immunological assay" alone or in combination, in English language...
October 2016: Iranian Journal of Parasitology
https://www.readbyqxmd.com/read/28122564/genetic-detection-of-peste-des-petits-ruminants-virus-under-field-conditions-a-step-forward-towards-disease-eradication
#12
Waqas Ashraf, Hermann Unger, Sunaina Haris, Ameena Mobeen, Muhammad Farooq, Muhammad Asif, Qaiser Mahmood Khan
BACKGROUND: The devastating viral disease of small ruminants namely Peste des petits ruminants (PPR) declared as target for "Global Eradication" in 2015 by the Food and Agriculture Organization (FAO) and the World Organization for Animal Health (OIE). For a successful eradication campaign, molecular diagnostic tools are preferred for their specificity, efficacy and robustness to compliment prophylactic measures and surveillance methods. However, molecular tools have a few limitations including, costly equipment, multi-step template preparation protocols, target amplification and analysis that restrict their use to the sophisticated laboratory settings...
January 25, 2017: BMC Veterinary Research
https://www.readbyqxmd.com/read/28118889/the-development-of-loop-mediated-isothermal-amplification-combined-with-lateral-flow-dipstick-for-detection-of-karlodinium-veneficum
#13
Hai-Long Huang, Peng Zhu, Cheng-Xu Zhou, Shan He, Xiao-Jun Yan
The aim of this study was to develop a loop-mediated isothermal amplification (LAMP) combined with a chromatographic lateral flow dipstick (LFD) assay to rapidly and specifically detect the Karlodinium veneficum ITS gene. Four groups of LAMP primers were specially designed to target the K. veneficum ITS gene. The LAMP-LFD detection limit was 7.4pg/μL (approximately 6.5cells/mL) of K. veneficum genomic DNA and was 10 times more sensitive than standard PCR. The LAMP-LFD method exhibited high specificity and accurately identified K...
February 2017: Harmful Algae
https://www.readbyqxmd.com/read/28118097/a-loop-mediated-isothermal-amplification-lamp-assay-targeting-16s-rrna-gene-for-rapid-detection-of-anaplasma-phagocytophilum-infection-in-sheep-and-goats
#14
Changshen Ning, Jinhong Wang, Yan Zhang, Xiaoxing Wang, Yanyan Cui, Yaqun Yan, Rongjun Wang, Fuchun Jian, Longxian Zhang
Anaplasma phagocytophilum is a zoonotic pathogen and the causative agent of human granulocytic anaplasmosis (HGA) in humans and tick-borne fever in various kinds of animals. In the present study, a loop-mediated isothermal amplification (LAMP) assay for rapid detection of A. phagocytophilum was developed using primers specific to 16S rRNA gene of this organism. The LAMP assay was performed at 65 C for 60 min and terminated at 80 C for 10 min. The optimal reaction conditions, under which no cross-reaction was observed with other closely related tick borne parasites (Anaplasma bovis, Anaplasma ovis, Theileria luwenshuni, Babesia motasi and Schistosoma japonicum) was established...
January 24, 2017: Journal of Parasitology
https://www.readbyqxmd.com/read/28112131/polymerase-chain-reaction-for-mycobacterium-tuberculosis-dna-detection-from-ocular-fluids-in-patients-with-various-types-of-choroiditis-in-a-referral-eye-center-in-india
#15
Jyotirmay Biswas, Mohmmad Salman Kazi, Vishvesh Ashokkumar Agarwal, Md Shahid Alam, K Lily Therese
AIMS: The aim of this study was to detect Mycobacterium tuberculosis (MTB) DNA with polymerase chain reaction (PCR) in aqueous or vitreous samples of patients suffering from choroiditis presumed to be infectious origin. SETTINGS AND DESIGN: Hospital-based, retrospective case-control study. SUBJECTS AND METHODS: In all, forty eyes of forty patients with choroiditis divided into two groups - Group A (serpiginous-like choroiditis, ampiginous choroiditis, multifocal choroiditis) and Group B (choroidal abscess, miliary tuberculosis (TB), choroidal tubercle) were analyzed retrospectively...
December 2016: Indian Journal of Ophthalmology
https://www.readbyqxmd.com/read/28107985/enhanced-sensitivity-for-detection-of-plasmodium-falciparum-gametocytes-by-magnetic-nanoparticles-combined-with-enzyme-substrate-system
#16
Tienrat Tangchaikeeree, Piamsiri Sawaisorn, Sangdao Somsri, Duangporn Polpanich, Chaturong Putaporntip, Pramuan Tangboriboonrat, Rachanee Udomsangpetch, Kulachart Jangpatarapongsa
The highly sensitive and specific detection of Pfg377 gene of Plasmodium falciparum gametocyte using Magnetic Nanoparticles PCR Enzyme-Linked Gene Assay (MELGA) was successfully developed. The MELGA included amplification of the Pfg377 gene by polymerase chain reaction (PCR) using magnetic nanoparticles (MNPs)-conjugated forward primer and biotinylated reverse primer, followed by post-analytical process using horseradish peroxidase (HRP)-conjugated streptavidin (SA). The complexes of MELGA product were incubated with the peroxidase substrate and hydrogen peroxide to produce the signal for colorimetric measurement...
March 1, 2017: Talanta
https://www.readbyqxmd.com/read/28098507/development-and-evaluation-of-a-loop-mediated-isothermal-amplification-lamp-assay-for-the-detection-of-haemonchus-contortus-in-goat-fecal-samples
#17
Xin Yang, Mingwei Qi, Zongze Zhang, Chong Gao, Chunqun Wang, Weiqiang Lei, Li Tan, Junlong Zhao, Rui Fang, Min Hu
Haemonchus contortus is one of the most significant strongylid nematodes infecting small ruminants and causes great economic losses to the livestock industry worldwide. Accurate diagnosis of H. contortus is crucial to control the infection. Traditional microscopic examinations are the most common methods for the diagnosis of H. contortus, but they are time-consuming and inaccurate. Molecular methods based on PCR are more accurate, but need expensive machines usually only used in the laboratory. Loop-mediated isothermal amplification (LAMP) is a rapid, simple, specific and sensitive method that has been widely used to detect viruses, bacteria and parasites...
January 18, 2017: Journal of Parasitology
https://www.readbyqxmd.com/read/28096624/genetic-variability-and-discrimination-of-low-doses-of-toxocara-spp-from-public-areas-soil-inferred-by-loop-mediated-isothermal-amplification-assay-as-a-field-friendly-molecular-tool
#18
Maryam Ozlati, Adel Spotin, Abbas Shahbazi, Mahmoud Mahami-Oskouei, Teimour Hazratian, Mohammad Adibpor, Ehsan Ahmadpour, Afsaneh Dolatkhah, Paria Khoshakhlagh
ABSTRACT: Aim: One of the main diagnostic problems of conventional polymerase chain reaction (PCR) is indiscrimination of low parasitic loads in soil samples. The aim of this study is to determine the genetic diversity and identification of Toxocara spp. from public areas soil inferred by loop-mediated isothermal amplification (LAMP) assay. MATERIALS AND METHODS: A total of 180 soil samples were collected from various streets and public parks of northwest Iran. The DNA of recovered Toxocara eggs were extracted and amplified by PCR and LAMP following ZnSO4 flotation technique...
December 2016: Veterinary World
https://www.readbyqxmd.com/read/28095434/field-evaluation-of-a-high-throughput-loop-mediated-isothermal-amplification-test-for-the-detection-of-asymptomatic-plasmodium-infections-in-zanzibar
#19
Berit Aydin-Schmidt, Ulrika Morris, Xavier C Ding, Irina Jovel, Mwinyi I Msellem, Daniel Bergman, Atiqul Islam, Abdullah S Ali, Spencer Polley, Iveth J Gonzalez, Andreas Mårtensson, Anders Björkman
BACKGROUND: New field applicable diagnostic tools are needed for highly sensitive detection of residual malaria infections in pre-elimination settings. Field performance of a high throughput DNA extraction system for loop mediated isothermal amplification (HTP-LAMP) was therefore evaluated for detecting malaria parasites among asymptomatic individuals in Zanzibar. METHODS: HTP-LAMP performance was evaluated against real-time PCR on 3008 paired blood samples collected on filter papers in a community-based survey in 2015...
2017: PloS One
https://www.readbyqxmd.com/read/28093275/reverse-transcription-loop-mediated-isothermal-amplification-for-rapid-detection-of-respiratory-syncytial-virus-directly-from-nasopharyngeal-swabs
#20
Johannes Hoos, Rebecca Marie Peters, Julia Tabatabai, Jürgen Grulich-Henn, Paul Schnitzler, Johannes Pfeil
Respiratory syncytial virus (RSV) is a common cause of lower respiratory tract infection in young infants and a major cause of nosocomial infection in pediatric care. Currently available RSV point-of-care tests are of limited sensitivity and relatively expensive. We developed and evaluated a novel RSV rapid test for use at point-of-care, based on reverse-transcription loop-mediated isothermal amplification (RT-LAMP) for direct testing of nasopharyngeal swab specimens. RT-LAMP can detect RSV within 30min, without the need for RNA extraction...
January 16, 2017: Journal of Virological Methods
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