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LAMP PCR

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https://www.readbyqxmd.com/read/29344968/a-novel-nested-polymerase-chain-reaction-assay-targeting-plasmodium-mitochondrial-dna-in-field-collected-anopheles-mosquitoes
#1
M Calzetta, E Perugini, G Seixas, C A Sousa, W M Guelbeogo, N Sagnon, A Della Torre, J Pinto, M Pombi, E Mancini
Sensitive techniques for the detection of Plasmodium (Aconoidasida: Plasmodiidae) sporozoites in field-collected malaria vectors are essential for the correct assessment of risk for malaria transmission. A real-time polymerase chain reaction (RT-PCR) protocol targeting Plasmodium mtDNA proved to be much more sensitive in detecting sporozoites in mosquitoes than the widely used enzyme-linked immunosorbent assay targeting Plasmodium circumsporozoite protein (CSP-ELISA). However, because of the relatively high costs associated with equipment and reagents, RT-PCRs are mostly used to assess the outcomes of experimental infections in the frame of research experiments, rather than in routine monitoring of mosquito infection in the field...
January 18, 2018: Medical and Veterinary Entomology
https://www.readbyqxmd.com/read/29343880/novel-application-of-loop-mediated-isothermal-amplification-for-rapid-detection-of-gene-translocation
#2
Ibu Matsuzaki, Hideto Iguchi, Yurina Mikasa, Hiromu Morishita, Katsuya Okuda, Keita Nakaguchi, Yuki Mori, Yoshifumi Iwahashi, Kenji Warigaya, Masakazu Fujimoto, Fumiyoshi Kojima, Shin-Ichi Murata
Identification of fusion genes in cancer is essential for pathological diagnosis and clinical therapy. Although methods for detection of fusion genes, such as fluorescence in situ hybridization (FISH) and real-time polymerase chain reaction (PCR), have been developed in last two decades, these methods are not ideal for detection of these genetic alterations owing to their high cost and time-consuming procedures. In this study, we developed novel application for detection of gene translocations using loop-mediated isothermal amplification (LAMP)...
December 26, 2017: Acta Histochemica et Cytochemica
https://www.readbyqxmd.com/read/29336956/reverse-transcription-loop-mediated-isothermal-amplification-for-species-specific-detection-of-tomato-chlorotic-spot-orthotospovirus
#3
Xuelian Sui, Shouan Zhang, Zujian Wu, Kai-Shu Ling
Tomato chlorotic spot orthotospovirus (TCSV) is an emerging orthotospovirus that can cause severe disease on tomato plants. There are at least four orthotospoviruses infecting tomato, and mixed infection of two or more orthotospoviruses in a single tomato plant is quite common in the field. With similarity in the symptomatology and cross serological reactivity among tomato-infecting orthotospoviruses, especially between TCSV and groundnut ringspot orthotospovirus (GRSV), the current serological tests could not achieve definite and accurate species-specific determination in disease diagnosis...
January 11, 2018: Journal of Virological Methods
https://www.readbyqxmd.com/read/29332654/evaluation-of-loop-mediated-isothermal-amplification-lamp-assay-in-the-diagnosis-of-tubercular-lymphadenitis-a-pilot-study
#4
Baijayantimala Mishra, Vinaykumar Hallur, Bijayini Behera, C Preetam, Priti Nanda Mishra, Jyotirmayee Turuk, Prasanta Raghab Mohapatra
Tubercular lymphadenitis (TBLA) contributes to 30-40% of extrapulmonary TB cases in the immunocompetent individuals and 40-50% in people with HIV. Current diagnostic methods for TBLA like Gene-Xpert or PCR are costly and conventional methods like fine needle aspiration cytology, histopathology lack sensitivity and specificity. Culture which is considered as gold standard require high turnaround time. Loop mediated isothermal amplification (LAMP) assay has been developed as a novel technique for nucleic acid amplification and has shown promising results in the diagnosis of pulmonary tuberculosis...
January 2018: Indian Journal of Tuberculosis
https://www.readbyqxmd.com/read/29332129/aspergillus-fumigatus-increased-par-2-expression-and-elevated-proinflammatory-cytokines-expression-through-the-pathway-of-par-2-erk1-2-in-cornea
#5
Yawen Niu, Guiqiu Zhao, Cui Li, Jing Lin, Nan Jiang, Chengye Che, Jie Zhang, Qiang Xu
Purpose: To determine the role of protease-activated receptor-2 (PAR-2) in cornea infected by Aspergillus fumigatus. Methods: PAR-2 was tested in normal and infected corneas of C57BL/6 mice. Mice corneas were infected with A. fumigatus with or without pretreatment of PAR-2 antagonist (FSLLRY-NH2). Polymorphonuclear neutrophilic leukocytes (PMNs) were stimulated with 75% ethanol-killed A. fumigatus with or without pretreatment of FSLLRY-NH2. Disease severity was documented by clinical score and photographs with a slit lamp...
January 1, 2018: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/29330674/advances-in-serological-imaging-techniques-and-molecular-diagnosis-of-toxoplasma-gondii-infection
#6
REVIEW
Ali Rostami, Panagiotis Karanis, Shirzad Fallahi
BACKGROUND: Toxoplasmosis is worldwide distributed zoonotic infection disease with medical importance in immunocompromised patients, pregnant women and congenitally infected newborns. Having basic information on the traditional and new developed methods is essential for general physicians and infectious disease specialists for choosing a suitable diagnostic approach for rapid and accurate diagnosis of the disease and, consequently, timely and effective treatment. METHODS: We conducted English literature searches in PubMed from 1989 to 2016 using relevant keywords and summarized the recent advances in diagnosis of toxoplasmosis...
January 12, 2018: Infection
https://www.readbyqxmd.com/read/29329617/dna-detection-of-trypanosoma-evansi-diagnostic-validity-of-a-new-assay-based-on-loop-mediated-isothermal-amplification-lamp
#7
Qunbo Tong, Rui Chen, Qingming Kong, Julie Goossens, Magdalena Radwanska, Di Lou, Jianzu Ding, Bin Zheng, Yixiu Fu, Tianping Wang, Magez Stefan, Shaohong Lu
Trypanosoma evansi (T. evansi) is the most widely spread pathogenic trypanosome in the world. The control of trypanosomiasis depends on accurate diagnosis and effective treatment. Focusing on the presence of T. evansi in Asia, we developed a detection assay based on tracing phosphate ions (Pi) generated during LAMP targeting the variant surface glycoprotein (VSG) gene of Rode Trypanozoon antigenic type 1.2 (RoTat 1.2 VSG). The diagnostic potential as well as the use of the assay as a test-of-cure method after berenil treatment, was assessed in mice at different time points of infection...
January 30, 2018: Veterinary Parasitology
https://www.readbyqxmd.com/read/29325863/future-high-flux-assays-on-pathogenic-virulent-and-toxic-genetics-from-various-pathogens
#8
REVIEW
Yanmei Li, Caiying Bai, Ling Yang, Jie Fu, Muxia Yan, Dingqiang Chen, Li Zhang
Toxins, encoding by virulence factors, are significant cause of food-borne illnesses and death in the worldwide. Loop-mediated isothermal amplification (LAMP) is one of the widely used methodologies because of the high sensitivity, specificity and rapidity. Nowadays, LAMP has been regarded as an innovative gene amplification technology and emerged as an alternative to PCR-based methodologies in identification of the pathogenic virulent and toxic genetics. The high sensitivity of LAMP enables detection of the pathogens in sample materials even without time consuming and sample preparation...
January 8, 2018: Microbial Pathogenesis
https://www.readbyqxmd.com/read/29317875/diagnostic-accuracy-of-loop-mediated-isothermal-amplification-assay-as-a-field-molecular-tool-for-rapid-mass-screening-of-old-world-leishmania-infections-in-sand-flies-and-in-vitro-culture
#9
Mehdi Ghodrati, Adel Spotin, Teimour Hazratian, Mahmoud Mahami-Oskouei, Ali Bordbar, Sahar Ebrahimi, Shirzad Fallahi, Parviz Parvizi
Background: We employed a highly sensitive loop-mediated isothermal amplification (LAMP) by targeting 18S rRNA gene to identify the rapid mass screening of Leishmania infections in captured sand flies of southwest Iran and In vitro culture. Methods: One hundred fifty sand flies were collected from 11 sites adjacent to Iraqi's borders in southern parts of Khuzestan Province by using sticky sheets of paper and CDC miniature light traps during late May 2014 to Nov 2015...
October 2017: Iranian Journal of Parasitology
https://www.readbyqxmd.com/read/29317206/exendin-4-impairs-the-autophagic-flux-to-induce-apoptosis-in-pancreatic-acinar-ar42j-cells-by-down-regulating-lamp-2
#10
Zhiqiang Li, Shaihong Zhu, Lihua Huang, Mingming Shang, Can Yu, Hongwei Zhu, Duo Han, Hui Huang, Xiao Yu, Xia Li
This study aimed to explore the mechanism of impaired autophagy flux induced by exendin-4 and its role on cell apoptosis in pancreatic AR42J cells. The AR42J cells were treated with various concentration of exendin-4 for several time points to assess its cytotoxicity by MTT assay. Then the AR42J cells were treated by 10pM exendin-4 for 72 h, the cell death was analyzed by flow cytometry and caspase-3 level was examined by Western blot with or without the pretreatment of z-VAD-fmk to testify whether exendin-4 induces the cell apoptosis...
January 6, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29306009/genotyping-and-high-flux-sequencing-of-the-bacterial-pathogenic-elements-integrons
#11
REVIEW
Yanmei Li, Ling Yang, Jie Fu, Muxia Yan, Dingqiang Chen, Li Zhang
Regarded as a common genetic element responsible for horizontal gene transfer and wide spread of antimicrobial resistance among a large variety of bacteria, integrons are commonly distributed and considered as a determinant in the acquisition and evolution of virulence and antibiotic resistance. To date, the surveillances of integrons have been widely conducted in clinic, community even husbandry. For exact and accurate integron screening, as well as resistant cassettes, reliable monitoring methods is need...
January 3, 2018: Microbial Pathogenesis
https://www.readbyqxmd.com/read/29291217/rapid-detection-of-tomato-leaf-curl-bengaluru-virus-through-loop-mediated-isothermal-amplification-assay
#12
R Arutselvan, M Krishna Reddy, T Makeshkumar
A loop-mediated isothermal amplification (LAMP) technique was employed to develop a simple and rapid method for the detection of tomato leaf curl Bangalore virus (ToLCBaV) in diseased plants of tomato (Solanum lycopersicum). Six sets of primers were designed for LAMP technique targeting the conserved AC1 region and successfully detected ToLCBaV. No reaction was detected in the tissues of healthy plants by either the LAMP or the polymerase chain reaction (PCR). The LAMP products can be visualized by presence or absence of turbidity and staining (0...
September 2017: Virusdisease
https://www.readbyqxmd.com/read/29284484/platform-independent-gene-expression-signature-differentiates-sessile-serrated-adenomas-polyps-and-hyperplastic-polyps-of-the-colon
#13
Yasir Rahmatallah, Magomed Khaidakov, Keith K Lai, Hannah E Goyne, Laura W Lamps, Curt H Hagedorn, Galina Glazko
BACKGROUND: Sessile serrated adenomas/polyps are distinguished from hyperplastic colonic polyps subjectively by their endoscopic appearance and histological morphology. However, hyperplastic and sessile serrated polyps can have overlapping morphological features resulting in sessile serrated polyps diagnosed as hyperplastic. While sessile serrated polyps can progress into colon cancer, hyperplastic polyps have virtually no risk for colon cancer. Objective measures, differentiating these types of polyps would improve cancer prevention and treatment outcome...
December 28, 2017: BMC Medical Genomics
https://www.readbyqxmd.com/read/29278789/portable-platform-for-rapid-in-field-identification-of-human-fecal-pollution-in-water
#14
Yu Sherry Jiang, Timothy E Riedel, Jessica A Popoola, Barrett R Morrow, Sheng Cai, Andrew D Ellington, Sanchita Bhadra
Human fecal contamination of water is a public health risk. However, inadequate testing solutions frustrate timely, actionable monitoring. Bacterial culture-based methods are simple but typically cannot distinguish fecal host source. PCR assays can identify host sources but require expertise and infrastructure. To bridge this gap we have developed a field-ready nucleic acid diagnostic platform and rapid sample preparation methods that enable on-site identification of human fecal contamination within 80 min of sampling...
December 13, 2017: Water Research
https://www.readbyqxmd.com/read/29277714/loop-mediated-isothermal-amplification-assay-for-the-rapid-and-visual-detection-of-the-novel-porcine-circovirus-3
#15
Yu-Ri Park, Hye-Ryung Kim, Seong-Hee Kim, Kyoung-Ki Lee, Young S Lyoo, Sang-Geon Yeo, Choi-Kyu Park
A loop-mediated isothermal amplification (LAMP) assay using hydroxynaphthol blue was developed for the rapid and visual detection of the capsid gene of porcine circovirus 3 (PCV3). The amplification could be completed in 40 min at 62 °C, and the results could be visually detected by the naked eye. The assay specifically amplified PCV3 DNA and not other porcine viral nucleic acids. The limit of detection of the assay was 50 PCV3 DNA copies, which was comparable to that of the real-time polymerase chain reaction (qPCR) and lower than that of conventional PCR...
December 22, 2017: Journal of Virological Methods
https://www.readbyqxmd.com/read/29258454/atypical-acute-retinal-necrosis-accompanied-by-terson-s-syndrome-a-case-report
#16
Jong Young Lee, Dong Yoon Kim, Hye Jin Lee, Jin Ho Jeong, Sung Pyo Park, Jin Young Kim
BACKGROUND: Acute retinal necrosis (ARN) has characterized by panuveitis, vitritis, severe vaso-occlusive vasculitis, and diffuse necrotizing retinitis. There are no case reports on atypical ARN combined with Terson's syndrome. Herein, we report a case of ARN with atypical clinical features combined with Terson's syndrome that we successfully treated by intravitreal ganciclovir injection. CASE PRESENTATION: A 64-year-old man visited our eye clinic with a complaint of decreased visual acuity in his right eye...
December 19, 2017: BMC Ophthalmology
https://www.readbyqxmd.com/read/29251261/diagnostic-accuracy-of-loop-mediated-isothermal-amplification-lamp-for-screening-patients-with-imported-malaria-in-a-non-endemic-setting
#17
Camille Ponce, Flora Kaczorowski, Thomas Perpoint, Patrick Miailhes, Alain Sigal, Etienne Javouhey, Yves Gillet, Laurent Jacquin, Marion Douplat, Karim Tazarourte, Véronique Potinet, Bruno Simon, Adeline Lavoignat, Guillaume Bonnot, Fatimata Sow, Anne-Lise Bienvenu, Stéphane Picot
BACKGROUND: Sensitive and easy-to-perform methods for the diagnosis of malaria are not yet available. Improving the limit of detection and following the requirements for certification are issues to be addressed in both endemic and non-endemic settings. The aim of this study was to test whether loop-mediated isothermal amplification of DNA (LAMP) may be an alternative to microscopy or real-time PCR for the screening of imported malaria cases in non-endemic area. RESULTS: 310 blood samples associated with 829 suspected cases of imported malaria were tested during a one year period...
2017: Parasite: Journal de la Société Française de Parasitologie
https://www.readbyqxmd.com/read/29247634/comparison-of-single-nucleotide-polymorphism-genotyping-of-cyp2c19-by-loop-mediated-isothermal-amplification-and-real-time-pcr-melting-curve-analysis
#18
K M Kwong, C C Tam, Raymond Chan, Stephen W L Lee, P Ip, Janette Kwok
BACKGROUND: CYP2C19, a member of cytochrome P450 enzymes, is involved in various drug metabolisms, such as Clopidogrel. Common Single Nucleotide Polymorphisms (SNPs) of CYP2C19 gene, CYP2C19*2 and CYP2C19*3, are liable for the poor metabolism of Clopidogrel. It is crucial to identify poor metabolizers for alternative treatment as poor metabolism of Clopidogrel has been shown to correlate with worse clinical outcome in acute coronary syndrome (ACS) patients. METHOD: A genotyping method, Loop-mediated isothermal amplification (LAMP) was employed in this study...
December 13, 2017: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/29243178/development-and-validation-of-a-loop-mediated-isothermal-amplification-assay-for-the-detection-of-mycoplasma-bovis-in-mastitic-milk
#19
Aqeela Ashraf, Muhammad Imran, Tahir Yaqub, Muhammad Tayyab, Wasim Shehzad, Claro N Mingala, Yung-Fu Chang
Mycoplasma mastitis is often difficult to control due to a lack of rapid and accurate diagnostic tools. The aim of the current study was to develop a loop-mediated isothermal amplification (LAMP) assay for the detection of Mycoplasma bovis (M. bovis) in mastitic milk. The assay was developed using primers designed for three different target genes: uvrC, 16S rRNA, and gyrB, and validated using mastitic milk samples previously found positive for the target pathogen. Specificity of the developed assay was determined by testing cross-reactivity of LAMP primers against closely related bovine mastitis bacterial pathogens...
December 14, 2017: Folia Microbiologica
https://www.readbyqxmd.com/read/29241766/integron-mediated-bacterial-resistance-and-virulence-on-clinical-pathogens
#20
Ding-Qiang Chen, Yue-Ting Jiang, Dong-Hua Feng, Shu-Xian Wen, Dan-Hong Su, Ling Yang
Integron was recognized as mobile elements responsible for the emergence and diffusion of antibiotic resistance, virulence and pathogenicity. The existence of resistant integron in pathogens may consequently lead to the increasing number of clinical failures in bacterial mediated diseases, as well as the expenses. In this study, a total of 22 clinical pathogens (including E. faecalis, S. aureus, K. pneumoniae, Enterobacter, P. aeruginosa and Acinetobacter) were subjected to the identification of class 1-class 3 integrons and drug resistant gene cassettes by high flux LAMP method...
December 11, 2017: Microbial Pathogenesis
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