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LAMP PCR

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https://www.readbyqxmd.com/read/28330805/photodynamic-therapeutic-efficacy-of-symmetrical-diiodinated-squaraine-in-in-vivo-skin-cancer-models
#1
M S Soumya, D Devi Gayathri, K M Shafeekh, Suresh Das, Annie Abraham
BACKGROUND: Photodynamic therapy (PDT) has clinical approval for use as a minimally invasive therapeutic procedure that is able to exert selective cytotoxic activity toward malignant cells. The dye selected for our study, symmetrical diiodinated squaraine, is one of the newly developed photosensitizers. The study is designed to determine the efficacy of PDT mediated by symmetrical diiodinated squaraine in skin tumor induced Swiss albino mice. METHODS: Skin tumor was induced in mice with dimethyl benzanthracene (DMBA) and croton oil...
March 19, 2017: Photodiagnosis and Photodynamic Therapy
https://www.readbyqxmd.com/read/28330752/prevalence-and-distribution-of-covert-mortality-nodavirus-cmnv-in-cultured-crustacean
#2
Qingli Zhang, TingTing Xu, Xiaoyuan Wan, Shuang Liu, Xiuhua Wang, Xiaoping Li, Xuan Dong, Bing Yang, Jie Huang
An emerging covert mortality nodavirus (CMNV) was proved to be the infectious agent of shrimp viral covert mortality disease (VCMD). Prevalence and distribution of CMNV were investigated by using the methods of reverse transcription loop-mediated isothermal amplification (RT-LAMP), nested reverse transcription PCR, gene sequencing, histopathology, in situ RNA hybridization (ISH) and transmission electron microscope (TEM) in this study. RT-LAMP results showed that CMNV positive samples appeared in the cultured crustaceans including Litopenaeus vannamei, Fenneropenaeus chinensis, Marsupenaeus japonicus, Penaeus monodon, and Macrobrachium rosenbergii, and mostly distributed the coastal provinces in China...
March 18, 2017: Virus Research
https://www.readbyqxmd.com/read/28324245/comparison-of-dna-microarray-loop-mediated-isothermal-amplification-lamp-and-real-time-pcr-with-dna-sequencing-for-identification-of-fusarium-spp-obtained-from-patients-with-hematologic-malignancies
#3
Marcela de Souza, Tetsuhiro Matsuzawa, Kanae Sakai, Yasunori Muraosa, Luzia Lyra, Ariane Fidelis Busso-Lopes, Anna Sara Shafferman Levin, Angélica Zaninelli Schreiber, Yuzuru Mikami, Tohoru Gonoi, Katsuhiko Kamei, Maria Luiza Moretti, Plínio Trabasso
The performance of three molecular biology techniques, i.e., DNA microarray, loop-mediated isothermal amplification (LAMP), and real-time PCR were compared with DNA sequencing for properly identification of 20 isolates of Fusarium spp. obtained from blood stream as etiologic agent of invasive infections in patients with hematologic malignancies. DNA microarray, LAMP and real-time PCR identified 16 (80%) out of 20 samples as Fusarium solani species complex (FSSC) and four (20%) as Fusarium spp. The agreement among the techniques was 100%...
March 21, 2017: Mycopathologia
https://www.readbyqxmd.com/read/28323152/loop-mediated-isothermal-amplification-lamp-a-novel-rapid-detection-platform-for-pathogens
#4
REVIEW
Yanmei Li, Penghui Fan, Shishui Zhou, Li Zhang
Foodborne bacterial infections and diseases have been considered to be a major threat for public health in the worldwide. Increased incidence of human diseases caused by foodborne pathogens have been correlated with growing world population and mobility. Loop-mediated isothermal amplification (LAMP) has been regarded as an innovative gene amplification technology and emerged as an alternative to PCR-based methodologies in both clinical laboratory and food safety testing. Nowadays, LAMP has been applied to detection and identification on pathogens from microbial diseases, as it showed significant advantage in high sensitivity, specificity and rapidity...
March 17, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28318383/detection-of-brucellosis-in-sika-deer-cervus-nippon-through-loop-mediated-isothermal-amplification-lamp
#5
Qianhong Liu, Jie Wei, Qingsong Sun, Ben Wang, Yuting Wang, Ying Hu, Wenrong Wu
Brucellosis (Brucella bovis) in sika deer ( Cervus nippon ) can cause enormous losses to stag breeding, especially in areas in which stag breeding has become an important industry. It also poses a threat to humans because it is a zoonotic disease. Use of the loop-mediated isothermal amplification (LAMP) assay has been poorly described in the diagnosis of brucellosis in deer. We developed a LAMP assay targeting the omp25 gene sequence to detect brucellosis (Brucella bovis) in sika deer. The reaction can be completed in 60 min at 63 C and, with a detection limit of 17 pg, it was more sensitive than conventional PCR, with its detection limit of 1...
March 20, 2017: Journal of Wildlife Diseases
https://www.readbyqxmd.com/read/28303825/evaluation-of-loop-mediated-isothermal-amplification-assay-for-rapid-diagnosis-of-acanthamoeba-keratitis
#6
Abhishek Mewara, Sumeeta Khurana, Shakila Yoonus, Kirti Megha, Parveen Tanwar, Amit Gupta, Rakesh Sehgal
BACKGROUND: The clinical features of Acanthamoeba keratitis (AK) are non-specific and closely resemble bacterial, viral and fungal keratitis. MATERIALS AND METHODS: We compared loop-mediated isothermal amplification (LAMP) with microscopy, non-nutrient agar (NNA) culture and polymerase chain reaction (PCR) in clinical suspects of AK. RESULTS: Of 52 clinical samples (42 AK suspects and 10 proven bacterial, viral or fungal keratitis), 3 were positive by direct microscopy (sensitivity 60%, confidence interval [CI]: 17%-92...
January 2017: Indian Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28302098/in-vivo-confocal-microscopy-of-pre-descemet-corneal-dystrophy-associated-with-x-linked-ichthyosis-a-case-report
#7
Hui Shi, Xiao-Feng Qi, Tao-Tao Liu, Qian Hao, Xiao-Hong Li, Ling-Ling Liang, Yi-Miao Wang, Zhi-Hua Cui
BACKGROUND: Pre-Descemet corneal dystrophy (PDCD) is characterized by the presence of numerous, tiny, polymorphic opacities immediately anterior to Descemet membrane, which is a rare form of corneal stromal dystrophy and hard to be diagnosed. In vivo confocal microscopy (IVCM) is a useful tool to examine the minimal lesions of the cornea at the cellular level. In this article, we report a rare case of PDCD associated with X-linked ichthyosis and evaluate IVCM findings. CASE PRESENTATION: We present a 34-year-old male Chinese patient with PDCD associated with X-linked ichthyosis...
March 16, 2017: BMC Ophthalmology
https://www.readbyqxmd.com/read/28290475/the-development-of-loop-mediated-isothermal-amplification-lamp-assays-for-the-rapid-authentication-of-five-forbidden-vegetables-in-strict-vegetarian-diets
#8
Meng-Shiou Lee, Ting-Ying Su, Yi-Yang Lien, Shyang-Chwen Sheu
Plant-based food ingredients such as garlic, Chinese leek, Chinese onion, green onion and onion are widely used in many cuisines around the world. However, these ingredients known as the "five forbidden vegetables" (FFVs) are not allowed in some vegetarian diets. In this study, a loop-mediated isothermal amplification (LAMP) assay was developed for the detection of FFVs using five respective LAMP primer sets. The specific primers targeted the ITS1-5.8S-ITS2 nuclear ribosomal DNA sequence regions among the five vegetables...
March 14, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28274126/-selection-of-laboratory-procedures-to-detect-toxigenic-by-the-2-step-method
#9
Yoko Tanino, Mai Kodama, Hiroomi Daicho, Yoshito Miyauchi, Towa Yasumoto, Yukiji Yamada, Noriko Kyotani, Satoko Kurahashi, Masaji Ushiyama, Takeshi Kimura, Toshiaki Komori, Yumiko Fujitomo, Masaki Nakanishi, Naohisa Fujita
The 2-step method is an algorithm to detect toxigenic Clostridium difficile. We herein compared the sensitivities and specificities of an enzyme immunoassay (toxin A/B-EIA), toxigenic culture (TC-EIA), Loop-Mediated Isothermal Amplification assay (LAMP), and Xpert C. difficile (Xpert) with the detection of the toxin B gene by a polymerase chain reaction (PCR). The results obtained showed that the sensitivities and specificities of toxin A/B-EIA, Xpert, TC-EIA, and LAMP were 30 and 100%, 87.2 and 100%, 97.5 and 89...
March 22, 2017: JARMAM: Journal of the Association for Rapid Method and Automation in Microbiology
https://www.readbyqxmd.com/read/28250618/utility-of-loop-mediated-isothermal-amplification-assay-polymerase-chain-reaction-and-elisa-for-diagnosis-of-leptospirosis-in-south-indian-patients
#10
Mallika Sengupta, Abhilash Kundavaram Paul Prabhakar, Sowmya Satyendra, David Thambu, Ooriapadickal Cherian Abraham, Veeraraghavan Balaji, Hua-Wei Chen, Chien-Chung Chao, Wei-Mei Ching, John Antony Jude Prakash
BACKGROUND: Leptospirosis is a zoonotic disease which requires laboratory diagnosis for confirmation. MATERIALS AND METHODS: In this study serum samples from adults with acute undifferentiated fever (duration ≤15 days) were tested for IgM antibodies to Leptospira by ELISA, PCR for rrs gene and loop-mediated isothermal amplification (LAMP) assay for LipL32 and LipL41. RESULTS: Among the 150 sera tested, three were positive by PCR, LAMP and IgM ELISA/modified Faines' criteria, two by only PCR; seven only by LAMP assay and forty fulfilled modified Faine's criteria (illness clinically compatible and IgM ELISA positive for leptospirosis)...
January 2017: Journal of Global Infectious Diseases
https://www.readbyqxmd.com/read/28246533/use-of-molecular-methods-for-the-rapid-mass-detection-of-schistosoma-mansoni-platyhelminthes-trematoda-in-biomphalaria-spp-gastropoda-planorbidae
#11
Roberta Lima Caldeira, Liana Konovaloffi Jannotti-Passos, Omar Dos Santos Carvalho
The low stringency-polymerase chain reaction (LS-PCR) and loop-mediated isothermal amplification (LAMP) assays were used to detect the presence of S. mansoni DNA in (1) Brazilian intermediate hosts (Biomphalaria glabrata, B. straminea, and B. tenagophila) with patent S. mansoni infections, (2) B. glabrata snails with prepatent S. mansoni infections, (3) various mixtures of infected and noninfected snails; and (4) snails infected with other trematode species. The assays showed high sensitivity and specificity and could detect S...
2017: Journal of Tropical Medicine
https://www.readbyqxmd.com/read/28218268/rapid-and-sensitive-diagnoses-of-dry-root-rot-pathogen-of-chickpea-rhizoctonia-bataticola-taub-butler-using-loop-mediated-isothermal-amplification-assay
#12
Raju Ghosh, Avijit Tarafdar, Mamta Sharma
Dry root rot (DRR) caused by the fungus Rhizoctonia bataticola (Taub.) Butler, is an emerging disease in chickpea. The disease is often mistaken with other root rots like Fusarium wilt, collar rot and black root rot in chickpea. Therefore, its timely and specific detection is important. Current detection protocols are either based on mycological methods or on protocols involving DNA amplification by polymerase chain reaction (PCR). Here we report the rapid and specific detection of R. bataticola using loop-mediated isothermal amplification (LAMP) assay targeting fungal specific 5...
February 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28211246/dna-amplification-in-the-field-move-over-pcr-here-comes-lamp
#13
Patricia L M Lee
It would not be an exaggeration to say that among molecular technologies, it is PCR (polymerase chain reaction) that underpins the discipline of molecular ecology as we know it today. With PCR, it has been possible to target the amplification of particular fragments of DNA, which can then be analysed in a multitude of ways. The capability of PCR to amplify DNA from a mere handful of copies further means that conservationists and ecologists are able to sample DNA unobtrusively and with minimal disturbance to the environment and the organisms of interest...
March 2017: Molecular Ecology Resources
https://www.readbyqxmd.com/read/28185353/human-vaginal-epithelial-cells-augment-autophagy-marker-genes-in-response-to-candida-albicans-infection
#14
Ankit Shroff, Roicy Sequeira, Kudumula Venkata Rami Reddy
PROBLEM: Autophagy plays an important role in clearance of intracellular pathogens. However, no information is available on its involvement in vaginal infections such as vulvo-vaginal candidiasis (VVC). VVC is intimately associated with the immune status of the human vaginal epithelial cells (VECs). The objective of our study is to decipher if autophagy process is involved during Candida albicans infection of VECs. METHODS OF STUDY: In this study, C. albicans infection system was established using human VEC line (VK2/E6E7)...
February 10, 2017: American Journal of Reproductive Immunology: AJRI
https://www.readbyqxmd.com/read/28177044/comparison-of-lamp-and-pcr-for-molecular-mass-screening-of-sand-flies-for-leishmania-martiniquensis-infection
#15
Saruda Tiwananthagorn, Hirotomo Kato, Ranchana Yeewa, Amontip Muengpan, Raxsina Polseela, Saovanee Leelayoova
BACKGROUND: Leishmaniasis caused by Leishmania martiniquensis infection has been reported in human and domestic animals of Martinique Island, Germany, Switzerland, USA, Myanmar and Thailand. The peculiar clinical features of disseminated cutaneous and visceral forms co-existence render the urgent need of specific diagnostic tool to identify the natural sand fly vectors for effective prevention and control strategies. Loop-mediated isothermal amplification (LAMP) of 18S rRNA gene as well as polymerase chain reaction (PCR) of minicircle kinetoplast DNA gene (PCR-mkDNA) have never been applied to detect L...
February 2017: Memórias do Instituto Oswaldo Cruz
https://www.readbyqxmd.com/read/28167125/pma-lamp-for-rapid-detection-of-escherichia-coli-and-shiga-toxins-from-viable-but-non-culturable-state
#16
Muxia Yan, Ling Xu, Hua Jiang, Zhenwen Zhou, Shishui Zhou, Li Zhang
In exposure to outer pressure, microorganisms are capable of entry into the Viable But Non-Culturable (VBNC) state, and thus survive under various elimination processing. The survival microorganisms may yield negative results on culturing, and cause false negative for this golden standard methodology. In this study, a novel PMA-LAMP assay on the detection of Enterohemorrhage E. coli and shiga toxins has been developed and evaluated, with further application on a number of food borne E. coli strains. LAMP primers were designed on the target of rfbe for Enterohemorrhage E...
February 3, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28166235/development-and-clinical-performance-of-high-throughput-loop-mediated-isothermal-amplification-for-detection-of-malaria
#17
Rushini S Perera, Xavier C Ding, Frank Tully, James Oliver, Nigel Bright, David Bell, Peter L Chiodini, Iveth J Gonzalez, Spencer D Polley
BACKGROUND: Accurate and efficient detection of sub-microscopic malaria infections is crucial for enabling rapid treatment and interruption of transmission. Commercially available malaria LAMP kits have excellent diagnostic performance, though throughput is limited by the need to prepare samples individually. Here, we evaluate the clinical performance of a newly developed high throughput (HTP) sample processing system for use in conjunction with the Eiken malaria LAMP kit. METHODS: The HTP system utilised dried blood spots (DBS) and liquid whole blood (WB), with parallel sample processing of 94 samples per run...
2017: PloS One
https://www.readbyqxmd.com/read/28155195/clinical-evaluation-of-the-isothermal-amplification-assays-for-the-detection-of-four-common-respiratory-viruses-in-children-with-pneumonia
#18
Hangyu Zhou, Mengchuan Zhao, Xinna Li, Dan Zhang, Shuaifeng Zhou, Chen Chen, Zhishan Feng, Xuejun Ma
Respiratory viruses are recognized as serious causes of morbidity and mortality in lower respiratory tract infections in patients. Isothermal amplification assays are increasingly used in their detection because of their rapidity, simplicity and cost-effectiveness, when compared to traditional molecular diagnostic methods. However, systematic assessment of these assays in the clinical settings is rarely reported. MEDLINE (Pubmed) searches were done analysing subject headings and words in the abstract related to isothermal amplification assay and virus...
February 2, 2017: Archives of Virology
https://www.readbyqxmd.com/read/28141595/the-role-of-mincle-in-innate-immune-to-fungal-keratitis
#19
Guiqiu Zhao, Qiang Xu, Jing Lin, Wenjun Chen, Tingting Cui, Liting Hu, Nan Jiang
INTRODUCTION: This study aimed to detect the early expression of macrophage-inducible C-type lectin (Mincle) and investigate its role in the innate immune response of fungal keratitis (FK). METHODOLOGY: Wistar rats were used to make fungal keratitis models. The inflammatory responses and corneal lesions were observed by slit-lamp microscope.RT-PCR, immunohistochemistry, and immunofluorescence were used to detect the expression of Mincle in the rat corneal epithelium...
January 30, 2017: Journal of Infection in Developing Countries
https://www.readbyqxmd.com/read/28140634/development-of-loop-mediated-isothermal-amplification-based-diagnostic-assays-for-detection-of-pasteurella-multocida-and-hemorrhagic-septicemia-associated-p-multocida-serotype-b-2
#20
Ahmed M Moustafa, Mark D Bennett
OBJECTIVE To develop 2 rapid loop-mediated isothermal amplification (LAMP) assays for detection of Pasteurella multocida DNA (Pm-LAMP assay) and P multocida DNA from strains associated with hemorrhagic septicemia (HS) in cattle and buffalo (HS-LAMP assay). SAMPLE Solutions containing 16 P multocida strains and 9 other bacterial species at various concentrations. PROCEDURES Optimal conditions were determined for running the Pm-LAMP and HS-LAMP assays. The assays were then used to detect DNA of the test organisms...
February 2017: American Journal of Veterinary Research
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