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LAMP PCR

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https://www.readbyqxmd.com/read/29776538/rapid-detection-of-calr-type-1-and-type-2-mutations-using-pna-lna-clamping-loop-mediated-isothermal-amplification-on-a-cd-like-microfluidic-chip
#1
Guojun Cao, Jilie Kong, Zhifang Xing, Yigui Tang, Xinju Zhang, Xiao Xu, Zhihua Kang, Xueen Fang, Ming Guan
Bleeding and thrombosis represent common complications in myeloproliferative neoplasms (MPN) and significantly contribute to morbidity and mortality. Molecular markers, including CALR mutations, were considered not only as diagnostic markers, but also as risk factors for bleeding and thrombosis associated with MPN, especially for patients in remote primary hospitals. We sought to develop an easy-to-use assay for the rapid detection of CALR type 1 (CALR-1) and type 2 (CALR-2) mutations in Philadelphia chromosome-negative MPN patients...
September 18, 2018: Analytica Chimica Acta
https://www.readbyqxmd.com/read/29765982/time-course-of-detection-of-human-male-dna-from-stained-blood-sample-on-various-surfaces-by-loop-mediated-isothermal-amplification-and-polymerase-chain-reaction
#2
Panan Kanchanaphum
This study explores determining the sex of humans from blood stains taken from different surfaces and compares the time course of detection with the conventional PCR, Conventional Loop Mediated Isothermal Amplification (LAMP), and LAMP-Lateral Flow Dipstick (LFD). For the DNA templates, 7 male and 7 female blood stained samples were extracted and added to LAMP and PCR reaction solution to amplify the SRY gene. The DNA samples were extracted from the following blood stained materials: cloth, wood, clay, and tile...
2018: BioMed Research International
https://www.readbyqxmd.com/read/29763668/evaluation-of-automated-loop-mediated-amplification-lamp-for-routine-malaria-detection-in-blood-samples-of-german-travelers-a-cross-sectional-study
#3
Hagen Frickmann, Rebecca Hinz, Sandra Rojak, Insa Bonow, Stefanie Ruben, Christine Wegner, Iris Zielke, Ralf Matthias Hagen, Egbert Tannich
BACKGROUND: We assessed a commercial loop-mediated amplification (LAMP) platform for its reliability as a screening tool for malaria parasite detection. METHODS: A total of 1000 blood samples from patients with suspected or confirmed malaria submitted to the German National Reference Center for Tropical Pathogens were subjected to LAMP using the Meridian illumigene Malaria platform. Results were compared with microscopy from thick and thin blood films in all cases...
May 12, 2018: Travel Medicine and Infectious Disease
https://www.readbyqxmd.com/read/29763640/development-of-fluorescent-reverse-transcription-loop-mediated-isothermal-amplification-rt-lamp-using-quenching-probes-for-the-detection-of-the-middle-east-respiratory-syndrome-coronavirus
#4
Kazuya Shirato, Shohei Semba, Sherif A El-Kafrawy, Ahmed M Hassan, Ahmed M Tolah, Ikuyo Takayama, Tsutomu Kageyama, Tsugunori Notomi, Wataru Kamitani, Shutoku Matsuyama, Esam Ibraheem Azhar
Clinical detection of Middle East respiratory syndrome (MERS) coronavirus (MERS-CoV) in patients is achieved using genetic diagnostic methods, such as real-time RT-PCR assay. Previously, we developed a reverse transcription-loop-mediated isothermal amplification (RT-LAMP) assay for the detection of MERS-CoV [Virol J. 2014. 11:139]. Generally, amplification of RT-LAMP is monitored by the turbidity induced by precipitation of magnesium pyrophosphate with newly synthesized DNA. However, this mechanism cannot completely exclude the possibility of unexpected reactions...
May 12, 2018: Journal of Virological Methods
https://www.readbyqxmd.com/read/29750967/evaluation-of-four-novel-isothermal-amplification-assays-towards-simple-and-rapid-genotyping-of-chloroquine-resistant-plasmodium-falciparum
#5
Madhvi Chahar, Anup Anvikar, Rajnikant Dixit, Neena Valecha
Loop mediated isothermal amplification (LAMP) assay is sensitive, prompt, high throughput and field deployable technique for nucleic acid amplification under isothermal conditions. In this study, we have developed and optimized four different visualization methods of loop-mediated isothermal amplification (LAMP) assay to detect Pfcrt K76T mutants of P. falciparum and compared their important features for one-pot in-field applications. Even though all the four tested LAMP methods could successfully detect K76T mutants of P...
May 8, 2018: Experimental Parasitology
https://www.readbyqxmd.com/read/29748557/recombinant-amelogenin-regulates-the-bioactivity-of-mouse-cementoblasts-in-vitro
#6
Sema S Hakki, S Buket Bozkurt, Emre Türkay, Michel Dard, Nuhan Purali, Werner Götz
Amelogenin (AMG) is a cell adhesion molecule that has an important role in the mineralization of enamel and regulates events during dental development and root formation. The purpose of the present study was to investigate the effects of recombinant human AMG (rhAMG) on mineralized tissue-associated genes in cementoblasts. Immortalized mouse cementoblasts (OCCM-30) were treated with different concentrations (0.1, 1, 10, 100, 1000, 10,000, 100,000 ng · mL-1 ) of recombinant human AMG (rhAMG) and analyzed for proliferation, mineralization and mRNA expression of bone sialoprotein (BSP), osteocalcin (OCN), collagen type I (COL I), osteopontin (OPN), runt-related transcription factor 2 (Runx2), cementum attachment protein (CAP), and alkaline phosphatase (ALP) genes using quantitative RT-PCR...
May 9, 2018: International Journal of Oral Science
https://www.readbyqxmd.com/read/29738236/a-smartphone-based-in-gel-loop-mediated-isothermal-amplification-glamp-system-enables-rapid-coliphage-ms2-quantification-in-environmental-waters
#7
Xiao Huang, Xingyu Lin, Katharina Urmann, Lijie Li, Xing Xie, Sunny Jiang, Michael R Hoffmann
Model coliphages (e.g., ΦX174, MS2, and PRD1) have been widely used in place of pathogenic viruses to study their fate and transport in the environment and during wastewater treatment. Two groups of coliphages (F-specific and somatic) are being explored as indicators of viral fecal pollution in ambient water. However, the detection and quantification of coliphages still largely relies on time-consuming culture-based plaque assays. In this study, we developed an in-gel loop mediated isothermal amplification (gLAMP) system enabling coliphage MS2 quantification within 30 minutes using standard laboratory devices...
May 8, 2018: Environmental Science & Technology
https://www.readbyqxmd.com/read/29708937/epithelial-recurrent-erosion-dystrophy-secondary-to-col17a1-c-3156c-t-mutation-in-a-non-white-family
#8
Farnoosh Vahedi, Doug D Chung, Katherine M Gee, Pichaya Chuephanich, Anthony J Aldave
PURPOSE: To report the identification of the collagen, type XVII, alpha 1 (COL17A1) c.3156C>T mutation associated with epithelial recurrent erosion dystrophy (ERED) in a Thai family. METHODS: Slit-lamp examination was performed to determine the affected status of each member of a Thai family, with multiple members demonstrating scattered Bowman layer opacities. After genomic deoxyribonucleic acid (DNA) was isolated from saliva, polymerase chain reaction (PCR) amplification and Sanger sequencing were performed to screen COL17A1 and exons 4 and 12 of the transforming growth factor β-induced gene...
April 26, 2018: Cornea
https://www.readbyqxmd.com/read/29708802/establishment-of-a-multiplex-loop-mediated-isothermal-amplification-method-for-rapid-detection-of-sulfonamide-resistance-genes-sul1-sul2-sul3-in-clinical-enterobacteriaceae-isolates-from-poultry
#9
Jiansen Gong, Linlin Zhuang, Di Zhang, Ping Zhang, Xinhong Dou, Chengming Wang
Antimicrobial resistance genes play an important role in mediating resistance to sulfonamide in Gram-negative bacteria. While PCR is the current method to detect sulfonamide resistance genes (sul1, sul2, sul3), it is time-consuming and costly and there is an urgent need to develop a more convenient, simpler and rapid test for the sul. In this study, we describe a multiplex loop-mediated isothermal amplification (m-LAMP) assay we developed for the rapid and simultaneous detection of three sul. This m-LAMP assay successfully detected seven reference strains with different sul genotypes, but was negative for nine sul-negative reference strains...
April 30, 2018: Foodborne Pathogens and Disease
https://www.readbyqxmd.com/read/29704305/exponential-isothermal-amplification-of-nucleic-acids-and-amplified-assays-for-proteins-cells-and-enzyme-activities
#10
Michael S Reid, X Chris Le, Hongquan Zhang
Isothermal exponential amplification techniques, such as strand-displacement amplification (SDA), rolling circle amplification (RCA), loop-mediated isothermal amplification (LAMP), nucleic acid sequence-based amplification (NASBA), helicase-dependent amplification (HDA), and recombinase polymerase amplification (RPA), have great potential for on-site, point-of-care, and in-situ assay applications. These amplification techniques eliminate the need for temperature cycling required for polymerase chain reaction (PCR) while achieving comparable amplification yield...
April 27, 2018: Angewandte Chemie
https://www.readbyqxmd.com/read/29701140/expression-of-plasma-hsa-mir122-in-hbv-related-hepatocellular-carcinoma-hcc-in-vietnamese-patients
#11
Nguyen Bao Quoc, Nguyen Doan Nguyen Phuong, Tang Kim Ngan, Nguyen Thi Minh Linh, Pham Hung Cuong, Nguyen Ngoc Bao Chau
BACKGROUND: Hepatocellular carcinoma (HCC) is the leading cause of cancer-related death in the world and considered as one of the most susceptible cancers in humans. The microRNA molecule, hsa-miR122, considered as a potential biological marker linked with the injury of hepatocellular tissue, is the most common microRNA in human liver cancer. Understanding the expression profile of hsa-miR122 plays an important role in the diagnosis of HCC Objective: Identification and comparison of cut-off values of plasma hsa-miR122 expression were conducted in blood samples of healthy control, HBV infected and HBV-related HCC Vietnamese patients Method and result: Fifty-two blood samples of healthy control and HBV-related HCC cases, collected between 2015 and 2017 were obtained from Ho Chi Minh City Oncology Hospital, Vietnam...
April 27, 2018: MicroRNA
https://www.readbyqxmd.com/read/29698659/unraveling-cryptic-epizootiology-of-equid-trypanosomosis-in-punjab-state-of-india-by-parasitological-and-sero-molecular-techniques
#12
Rahul Parashar, L D Singla, Kanisht Batra, Rajendra Kumar, Neeraj Kashyap, Paramjit Kaur, M S Bal
To unravel equid trypanosomosis caused by Trypanosoma evansi in Punjab state of India, a cross sectional study was designed by utilizing parasitological and sero-molecular tools with objective to assess the prevalence of T. evansi in association with various risk factors in all agroclimatic zones of Punjab state of India. Parasitological Romanowksy stained thin blood smears (RSTBS) to detect patent infection, molecular techniques polymerase chain reaction I (PCR I; TBR 1/2 primers; targeting minichromosomal satellite DNA of T...
April 23, 2018: Acta Tropica
https://www.readbyqxmd.com/read/29698484/rapid-detection-of-puccinia-triticina-causing-leaf-rust-of-wheat-by-pcr-and-loop-mediated-isothermal-amplification
#13
C Manjunatha, Sapna Sharma, Deepika Kulshreshtha, Sangeeta Gupta, Kartar Singh, Subhash C Bhardwaj, Rashmi Aggarwal
Leaf rust of wheat caused by Puccinia triticina has significant impact on wheat production worldwide. Effective and quick detection methodologies are required to mitigate yield loss and time constraints associated with monitoring and management of leaf rust of wheat. In the present study, detection of P. triticina has been simplified by developing a rapid, reliable, efficient and visual colorimetric method i.e., loop mediated isothermal amplification of DNA (LAMP). Based on in silico analysis of P. triticina genome, PTS68, a simple sequence repeat was found highly specific to leaf rust fungus...
2018: PloS One
https://www.readbyqxmd.com/read/29696335/an-effective-established-biosensor-of-bifunctional-probes-labeled-aunps-combined-with-lamp-for-detection-of-fish-pathogen-streptococcus-iniae
#14
Ya Zhou, Jingfan Xiao, Xin Ma, Qiyao Wang, Yuanxing Zhang
In purpose of valid Streptococcus iniae detection, we established a colorimetric biosensor using gold nanoparticles (AuNPs) labeled with dual functional probes and along with loop-mediated isothermal amplification (LAMP) assay (LAMP-AuNPs). Based on the characteristics of self-aggregation and bio-conjugation with ligands, AuNPs were chosen for observable color change in tandem with LAMP amplification method to reach high sensitivity and easy operation. Meanwhile, the improvement of dual probes that could fully utilize the LAMP product gave the biosensor a stable result exhibition...
April 25, 2018: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/29696299/development-of-mrna-based-body-fluid-identification-using-reverse-transcription-loop-mediated-isothermal-amplification
#15
Tetsuya Satoh, Seiya Kouroki, Keita Ogawa, Yorika Tanaka, Kazutoshi Matsumura, Susumu Iwase
Identifying body fluids from forensic samples can provide valuable evidence for criminal investigations. Messenger RNA (mRNA)-based body fluid identification was recently developed, and highly sensitive parallel identification using reverse transcription polymerase chain reaction (RT-PCR) has been described. In this study, we developed reverse transcription loop-mediated isothermal amplification (RT-LAMP) as a simple, rapid assay for identifying three common forensic body fluids, namely blood, semen, and saliva, and evaluated its specificity and sensitivity...
April 25, 2018: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/29692952/development-of-a-quantitative-loop-mediated-isothermal-amplification-assay-for-the-field-detection-of-erysiphe-necator
#16
Lindsey D Thiessen, Tara M Neill, Walter F Mahaffee
Plant pathogen detection systems have been useful tools to monitor inoculum presence and initiate management schedules. More recently, a loop-mediated isothermal amplification (LAMP) assay was successfully designed for field use in the grape powdery mildew pathosystem; however, false negatives or false positives were prevalent in grower-conducted assays due to the difficulty in perceiving the magnesium pyrophosphate precipitate at low DNA concentrations. A quantitative LAMP (qLAMP) assay using a fluorescence resonance energy transfer-based probe was assessed by grape growers in the Willamette Valley of Oregon...
2018: PeerJ
https://www.readbyqxmd.com/read/29681951/lateral-flow-loop-mediated-isothermal-amplification-test-with-stem-primers-detection-of-cryptosporidium-species-in-kenyan-children-presenting-with-diarrhea
#17
Timothy S Mamba, Cecilia K Mbae, Johnson Kinyua, Erastus Mulinge, Gitonga Nkanata Mburugu, Zablon K Njiru
Background. Cryptosporidium is a protozoan parasite and a major cause of diarrhea in children and immunocompromised patients. Current diagnostic methods for cryptosporidiosis such as microscopy have low sensitivity while techniques such as PCR indicate higher sensitivity levels but are seldom used in developing countries due to their associated cost. A loop-mediated isothermal amplification (LAMP) technique, a method with shorter time to result and with equal or higher sensitivity compared to PCR, has been developed and applied in the detection of Cryptosporidium species...
2018: Journal of Tropical Medicine
https://www.readbyqxmd.com/read/29675368/mincle-in-the-innate-immune-response-of-mice-fungal-keratitis
#18
Guo-Rong Yu, Jing Lin, Jie Zhang, Cheng-Ye Che, Xu-Dong Peng, Cui Li, Li-Ting Hu, Guo-Qiang Zhu, Kun He, Gui-Qiu Zhao
AIM: To investigate how macrophage inducible C-type lectin (Mincle) influences inflammation in mice fungal keratitis induced by Aspergillus fumigatus ( A. fumigatus ). METHODS: C57BL/6 mice were infected with A. fumigatus after pretreated with Mincle agonist TDB or Mincle neutralizing antibody (MincleAb), taking DMSO or IgG as control group respectively. The cornea lesions were monitored with slit-lamp microscope and evaluated by clinical score. Mincle expression was assessed using reverse transcription-ploymerase chain reaction (RT-PCR) and immunostaining...
2018: International Journal of Ophthalmology
https://www.readbyqxmd.com/read/29665825/evaluation-of-fluorimetry-and-direct-visualization-to-interpret-results-of-a-loop-mediated-isothermal-amplification-kit-to-detect-leishmania-dna
#19
Ana V Ibarra-Meneses, Israel Cruz, Carmen Chicharro, Carmen Sánchez, Sylvain Biéler, Tobias Broger, Javier Moreno, Eugenia Carrillo
BACKGROUND: Nucleic acid amplification tests (NAATs) have proven to be advantageous in the diagnosis of leishmaniases, allowing sensitive diagnosis of: (i) cutaneous leishmaniasis in long duration lesions and (ii) visceral leishmaniasis using a less-invasive sample like peripheral blood, in opposition to tissue aspiration required for parasite demonstration by microscopy. Despite their benefits, the implementation of NAATs for leishmaniasis diagnosis at the point-of-care has not been achieved yet, mostly due to the complexity and logistical issues associated with PCR-based methods...
April 17, 2018: Parasites & Vectors
https://www.readbyqxmd.com/read/29657238/the-first-report-of-terbinafine-resistance-microsporum-canis-from-a-cat
#20
Yun-Hsia Hsiao, Charles Chen, Hock Siew Han, Rui Kano
A 2-year-old, exotic shorthair cat presented with baldness and mild scaling on trunk that was confirmed as Microsporum canis (M. canis) infection by the following methods. Wood's lamp and trichogram were used to demonstrate fungal elements suggestive of dermatophytosis consistent with M. canis. Dermatophyte test medium (DTM) and polymerase chain reaction (PCR) were used for identification. E-test and broth microdilution test were then utilized to estimate antifungal minimal inhibitory concentrations (MICs) towards ITZ and TRF respectively...
April 16, 2018: Journal of Veterinary Medical Science
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