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cecal ligation and. puncture

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https://www.readbyqxmd.com/read/29664837/chronic-alcohol-ingestion-worsens-survival-and-alters-gut-epithelial-apoptosis-and-cd8-t-cell-function-after-pseudomonas-aeruginosa-pneumonia-induced-sepsis
#1
Nathan J Klingensmith, Katherine T Fay, John D Lyons, Ching-Wen Chen, Shunsuke Otani, Zhe Liang, Deena B Chihade, Eileen M Burd, Mandy L Ford, Craig M Coopersmith
Mortality is higher in septic patients with a history of alcohol use disorder than in septic patients without a history of chronic alcohol usage. We have previously described a model of chronic alcohol ingestion followed by sepsis from cecal ligation and puncture in which alcohol-fed septic mice have higher mortality than water-fed septic mice, associated with altered gut integrity and increased production of TNF and IFNγ by splenic CD4 T cells without alterations in CD8 T cell function. The purpose of this study was to determine whether this represents a common host response to the combination of alcohol and sepsis by creating a new model in which mice with chronic alcohol ingestion were subjected to a different model of sepsis...
April 16, 2018: Shock
https://www.readbyqxmd.com/read/29664835/phagocytosis-by-fibrocytes-as-a-mechanism-to-decrease-bacterial-burden-and-increase-survival-in-sepsis
#2
Dalis Collins, Christopher Fry, Bethany B Moore, Jean A Nemzek
Fibrocytes are unique cells with innate and adaptive immune functions, but these mechanisms have not been fully explored. The aim of this study was to explain the mechanism by which adoptive transfer of exogenous fibrocytes improved bacterial clearance and increased sepsis survival. Initial flow cytometry-based, in vitro assays demonstrated phagocytosis by fibrocytes and intracellular bacterial killing was confirmed by direct plating of cell lysates after exposure to live bacteria. Intravenous adoptive transfer of fibrocytes at the time of cecal ligation and puncture (CLP) or two hours after CLP in mice increased survivability...
April 16, 2018: Shock
https://www.readbyqxmd.com/read/29662678/interleukin-6-is-a-key-factor-for-immunoglobulin-like-transcript-4-mediated-immune-injury-in-sepsis
#3
De Wen Zhang, Jian He
Background: ILT4+ monocytes seem to be associated with poor prognosis of sepsis in humans, but the exact mechanisms are unknown. This study aimed to examine the biological behaviors and effects of immunoglobulin-like transcript-4 (ILT4) levels on monocytes during sepsis and on the prognosis of sepsis. Methods: ILT4+/+ (WT) and ILT4-knockout (ILT4-/- ) male BALB/c mice were used for sepsis modeling using cecal ligation puncture (CLP). Flow cytometry was used to measure the levels of ILT4 and major histocompatibility complex class II (MHC-II) on peripheral blood monocytes 24 h after CLP...
2018: Journal of Intensive Care
https://www.readbyqxmd.com/read/29655793/inhibition-of-nlrp9b-attenuates-acute-lung-injury-through-suppressing-inflammation-apoptosis-and-oxidative-stress-in-murine-and-cell-models
#4
Qiu Yanling, Cheng Xiaoning, Bai Fei, Fang Liyun, Hu Huizhong, Sun Daqing
Acute lung injury (ALI), known a severe disease along with high morbidity and mortality, is lacking of specific therapies. Inflammation, apoptosis and oxidative stress are critical pathologies that contribute to ALI. Recently, there is study indicated that NLRP9b, a NOD-like receptor (NLR) member, is critical in modulation of inflammatory response. However, the effects of NLRP9b on sepsis-associated ALI, and the underlying molecular mechanism have not been understood. In the present study, the wild type (WT) and NLRP9b-knockout (NLRP9b-/- ) mice with C57B/L6 background were subjected to a cecal ligation and puncture (CLP) for ALI murine model establishment...
April 12, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29651245/antiseptic-activity-of-ethnomedicinal-xuebijing-revealed-by-the-metabolomics-analysis-using-uhplc-q-orbitrap-hrms
#5
Lihua Zuo, Lin Zhou, Tanye Xu, Zhuolun Li, Liwei Liu, Yingying Shi, Jian Kang, Guanmin Gao, Shuzhang Du, Zhi Sun, Xiaojian Zhang
Xuebijing (XBJ) injection is an ethnomedicinal formula that has been widely used in the therapy of sepsis in China. However, the underlying theraputic mechanisms remain uninvestigated. In this research, a metabolomic method based on UHPLC-Q-Orbitrap HRMS was applied to make a holistic evaluation of XBJ on septic rats which were induced by the classical cecal ligation and puncture (CLP) operation. The plasma metabolic changes were profiled and evaluated by multivariate analytical (MVA) methods. In the results, a total of 41 differential metabolites were identified between CLP-operated group and sham-operated group, which were mainly involved in amino acid metabolism and lipid metabolism...
2018: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/29644918/assessment-of-clinical-sepsis-associated-biomarkers-in-a-septic-mouse-model
#6
Jin-Ling Li, Ge Li, Xi-Zhong Jing, Yun-Feng Li, Qiu-Ying Ye, Huan-Huan Jia, Shu-Hua Liu, Xue-Jiao Li, Hang Li, Ren Huang, Yu Zhang, Hui Wang
Objective Clinical sepsis-associated biomarkers were utilized in a cecal ligation and puncture (CLP) septic mouse model to provide a reference for investigating pathophysiological mechanisms and evaluating novel therapeutic interventions for sepsis. Methods Sepsis in mice was induced by CLP, and clinical biomarkers were evaluated (survival rate, blood physiological and biochemical indices, cytokines, hepatorenal function parameters, and blood coagulation). Results The mortality rate was >70%. The body temperature, blood pressure, and heart rate decreased within 48 h...
January 1, 2018: Journal of International Medical Research
https://www.readbyqxmd.com/read/29621118/bone-marrow-cells-transplant-in-septic-mice-modulates-systemic-inflammatory-response-via-cell-cell-contact
#7
Clara Batista Lorigados, Suely K Kubo Ariga, Thais Martins de Lima, Denise Frediane Barbeiro, Jose Eduardo Krieger, Francisco Garcia Soriano
Sepsis is a dynamic disease, displaying an inflammatory profile that varies over time and for each organ. Controlling the inflammatory response based in targeting a single molecule has been proved useless. We hypothesized that treatment with bone marrow derived mononuclear cells (BMDMC) may be more efficient to modulate the systemic inflammatory response to infection. Adult male Balb/c mice were subjected to cecal ligation and puncture (CLP) or endotoxemia model of experimental sepsis. BMDMC were separated under Ficoll gradient and injected intravenously 1 hour after the procedures...
April 4, 2018: Shock
https://www.readbyqxmd.com/read/29620245/hippocampal-%C3%AE-2%C3%A2-microglobulin-mediates-sepsis%C3%A2-induced-cognitive-impairment
#8
Rong Gao, Guomin Li, Runhua Yang, Hongmei Yuan, Shaogang Zhang
Acute brain dysfunction is a frequent complication in sepsis patients and is associated with long‑term neurocognitive consequences and increased mortality, yet the underlying mechanism remains unclear. Emerging evidence has suggested that β2‑microglobulin [a component of major histocompatibility complex (MHC) class I molecules] is involved in cognitive dysfunction in various neurological diseases. Therefore, the present study tested the hypothesis that β2‑microglobulin in the brain also mediates sepsis‑induced cognitive impairment...
April 5, 2018: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29617417/role-of-protein-kinase-c-delta-in-regulating-platelet-activation-and-platelet-leukocyte-interaction-during-sepsis
#9
Elisabetta Liverani, Mark J Mondrinos, Shuang Sun, Satya P Kunapuli, Laurie E Kilpatrick
Sepsis is characterized by an intense systemic inflammatory response activating a cascade of proinflammatory events resulting in leukocyte dysregulation and host tissue damage. The lung is particularly susceptible to systemic inflammation, leading to acute lung injury. Key to inflammation-induced lung damage is the excessive migration of neutrophils across the vascular endothelium. The mechanisms which regulate neutrophil activation and migration in sepsis are not well defined but there is growing evidence that platelets are actively involved and play a key role in microvascular permeability and neutrophil-mediated organ damage...
2018: PloS One
https://www.readbyqxmd.com/read/29608550/amitriptyline-treatment-mitigates-sepsis-induced-tumor-necrosis-factor-expression-and-coagulopathy
#10
Brent T Xia, Nadine Beckmann, Leah K Winer, Young Kim, Holly S Goetzman, Rosalie E Veile, Erich Gulbins, Michael D Goodman, Vanessa Nomellini, Charles C Caldwell
During sepsis, the early innate response and inflammatory cytokine cascade are associated with activation of the coagulation cascade. Acute hypercoagulability can contribute to lethal sequela of vascular thrombosis, tissue ischemia, and organ failure. We investigated if amitriptyline (AMIT), an antidepressant drug with a number of anti-inflammatory effects, could ameliorate sepsis in a murine model of sepsis - cecal ligation and puncture (CLP). We hypothesized that AMIT treatment would reduce inflammation and mitigate sepsis-induced coagulopathy...
March 30, 2018: Shock
https://www.readbyqxmd.com/read/29606867/nacl-for-the-safer-in-vivo-use-of-antibacterial-silver-based-nanoparticles
#11
Mingzhuo Liu, Huiqing Zhang, Xiangwei Song, Chaochao Wei, Zhenfang Xiong, Fen Yu, Chen Li, Fanrong Ai, Guanghua Guo, Xiaolei Wang
Background: As antibiotics progressively cease to be effective, silver based nanoparticles (SBNs), with broad antibacterial spectrum, might be the last line of defense against malicious bacteria. Unfortunately, there are still no proper SBNs-based strategies for in vivo antibacterial therapies. In this article, new carbon membrane packaged Ag nanoparticles (Ag-C) were synthesized. We assessed the effect of Ag-C with NaCl on size, cytotoxicity, antibacterial properties, metabolism and sepsis models...
2018: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/29604364/the-p75-neurotrophin-receptor-might-mediate-sepsis-induced-synaptic-and-cognitive-impairments
#12
Muhuo Ji, Hong-Mei Yuan, Shan-Wu Feng, Jiangyan Xia, Jianjun Yang
Systemic inflammation induces cognitive impairment, yet the mechanism involved in this process is unclear. Neurotrophin receptor p75 (p75NTR ) signaling is a key pathological factor contributing to neurobehavioral abnormalities in many neurodegenerative diseases. However, the role of p75NTR signaling in the regulation of sepsis-induced cognitive impairment remains largely to be elucidated. In this study, systemic inflammation was induced by cecal ligation and puncture (CLP). Neurobehavioral performances were evaluated by open field, novel object recognition, and fear conditioning tests...
March 28, 2018: Behavioural Brain Research
https://www.readbyqxmd.com/read/29601323/early-phase-innate-immune-suppression-in-murine-severe-sepsis-is-restored-with-systemic-interferon-%C3%AE
#13
Yoshiomi Kusakabe, Kanji Uchida, Yoshiro Yamamura, Takahiro Hiruma, Tokie Totsu, Yuho Tamai, Hitoshi Tsuyuzaki, Kyoko Hasegawa, Kyungho Chang, Yoshitsugu Yamada
BACKGROUND: Sepsis is a leading cause of death in the intensive care unit. Immune modulatory therapy targeting sepsis-associated proinflammatory responses has not shown survival benefit. Here, the authors evaluated innate immunity at the early stage of murine mild or severe peritoneal sepsis induced by cecal ligation and puncture, and the effect of systemic interferon-β, a potent inflammatory mediator, on severe sepsis as well as its mechanism of action. METHODS: Mild and severe sepsis was induced in C57BL/6 mice by cecal ligation and puncture with 22- and 18-gauge needles for puncture, respectively...
March 30, 2018: Anesthesiology
https://www.readbyqxmd.com/read/29599080/exosomes-from-endothelial-progenitor-cells-improve-the-outcome-of-a-murine-model-of-sepsis
#14
Yue Zhou, Pengfei Li, Andrew J Goodwin, James A Cook, Perry V Halushka, Eugene Chang, Hongkuan Fan
Microvascular dysfunction leads to multi-organ failure and mortality in sepsis. Our previous studies demonstrated that administration of exogenous endothelial progenitor cells (EPCs) confers protection in sepsis as evidenced by reduced vascular leakage, improved organ function, and increased survival. We hypothesize that EPCs protect the microvasculature through the exosomes-mediated transfer of microRNAs (miRNAs). Mice were rendered septic by cecal ligation and puncture (CLP), and EPC exosomes were administered intravenously at 4 hr after CLP...
February 27, 2018: Molecular Therapy: the Journal of the American Society of Gene Therapy
https://www.readbyqxmd.com/read/29589840/the-atypical-chemokine-receptor-ackr2-is-protective-against-sepsis
#15
Fernanda Vargas E Silva Castanheira, Vanessa Borges, Fabiane Sônego, Alexandre Kanashiro, Paula Barbim Donate, Paulo Henrique Melo, Kenneth Pallas, Remo Castro Russo, Flávio Almeida Amaral, Mauro Martins Teixeira, Fernando Silva Ramalho, Thiago Mattar Cunha, Foo Y Liew, José Carlos Alves-Filho, Gerard J Graham, Fernando Queiroz Cunha
Sepsis is a systemic inflammatory response as a result of uncontrolled infections. Neutrophils are the first cells to reach the primary sites of infection and chemokines play a key role in recruiting neutrophils. However, in sepsis chemokines could also contribute to neutrophil infiltration to vital organs leading to multiple organ failure. ACKR2 is an atypical chemokine receptor, which can remove and degrade inflammatory CC chemokines. The role of ACK2 in sepsis is unknown. Using a model of cecal ligation and puncture (CLP), we demonstrate here that ACKR2 deficient () mice exhibited a significant reduction in the survival rate compared to similarly treated wild type (WT) mice...
August 24, 2017: Shock
https://www.readbyqxmd.com/read/29577952/lipid-transfer-protein-isolated-from-noni-seeds-displays-antibacterial-activity-in-vitro-and-improves-survival-in-lethal-sepsis-induced-by-clp-in-mice
#16
Adson A Souza, Andrea S Costa, Dyély C O Campos, Andressa H M Batista, Gleilton W P Sales, Nádia A P Nogueira, Keila M M Alves, Andrelina Noronha Coelho-de-Souza, Hermógenes D Oliveira
In the present study, we aimed to evaluate the antibacterial activity of a lipid transfer protein isolated from Morinda citrifolia L. seeds, named McLTP1 , and to investigate its effect in the cecal ligation and puncture (CLP) mouse sepsis model. Antimicrobial assays revealed that McLTP1 (12.5-800 μg/mL) significantly reduced Staphylococcus aureus (ATCC 6538P and ATCC 14458) and Staphylococcus epidermidis (ATCC 12228) planktonic growth, reaching maximal inhibition of approximately 50% and 98%, respectively...
March 22, 2018: Biochimie
https://www.readbyqxmd.com/read/29568928/oral-supplementation-with-ursolic-acid-ameliorates-sepsis-induced-acute-kidney-injury-in-a-mouse-model-by-inhibiting-oxidative-stress-and-inflammatory-responses
#17
Zhenyu Zhang, Hong Zhang, Rui Chen, Zhong Wang
Ursolic acid (UA) as a multiple bioactive native compound has recently been demonstrated to treat sepsis in animal models. However, the beneficial effects of UA in sepsis‑induced acute kidney injury (AKI) are not completely understood. In the present study, the effect of UA on sepsis‑induced AKI in cecal ligation and puncture (CLP) surgery mice was investigated. Renal histomorphological analysis was performed by hematoxylin and eosin staining. The expression of inflammatory markers in the kidney of septic mice was measured by reverse transcription‑quantitative polymerase chain reaction and western blotting...
March 16, 2018: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29563990/insulin-alleviates-mitochondrial-oxidative-stress-involving-upregulation-of-superoxide-dismutase-2-and-uncoupling-protein-2-in-septic-acute-kidney-injury
#18
Guang-Dao Chen, Jun-Liang Zhang, Yi-Ting Chen, Ju-Xing Zhang, Tao Wang, Qi-Yi Zeng
The aim of the present study was to explore the effects and mechanisms of insulin on mitochondrial oxidative stress in septic acute kidney injury (AKI). Male Sprague Dawley rats were divided randomly into four groups: Control group, sham surgery group, cecal ligation and puncture (CLP) group, and CLP plus insulin group. Blood specimens and kidney tissues were obtained at 12 and 24 h after surgery as separate experiments. Analyses of histology and indicators of renal injury [blood urea nitrogen (BUN) and serum creatinine (CRE) and neutrophil gelatinase-associated lipocalin (NGAL)], mitochondrial function [adenosine triphosphate (ATP) and mitochondrial membrane potential (MMP)], oxidative stress [inducible nitric oxide synthase (iNOS), reactive oxygen species (ROS) and nitric oxide (NO)], endogenous antioxidant systems [superoxide dismutase (SOD) and glutathione (GSH)] as well as the expression of uncoupling protein (UCP), PINK1 protein (a major mediator of mitophagy), PGC1α protein (a major regulator of mitochondrial biogenesis) were performed...
April 2018: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/29563120/platelet-hmgb1-is-required-for-efficient-bacterial-clearance-in-intra-abdominal-bacterial-sepsis-in-mice
#19
Hui Zhou, Meihong Deng, Yingjie Liu, Chenxuan Yang, Rosemary Hoffman, Jingjiao Zhou, Patricia A Loughran, Melanie J Scott, Matthew D Neal, Timothy R Billiar
Thrombocytopenia impairs host defense and hemostasis in sepsis. However, the mechanisms of how platelets regulate host defense are not fully understood. High-mobility group box 1 (HMGB1), a danger-associated molecular pattern protein, is released during infection and contributes to the pathogenesis of sepsis. Platelets express HMGB1, which is released on activation and has been shown to play a critical role in thrombosis, monocyte recruitment, and neutrophil extracellular trap (NET) production. However, the contribution of platelet HMGB1 to host defense is unknown...
March 27, 2018: Blood Advances
https://www.readbyqxmd.com/read/29549730/ziziphus-spina-christi-l-leaf-extract-alleviates-myocardial-and-renal-dysfunction-associated-with-sepsis-in-mice
#20
Mohamed A Dkhil, Rami B Kassab, Saleh Al-Quraishy, Mohamed M Abdel-Daim, Rafat Zrieq, Ahmed E Abdel Moneim
Ziziphus spina-christi (L.), a traditional Arabian medicinal herb, has been used by Egyptians (Bedouin and Nubian) to treat inflammatory symptoms and swellings, pain, and heat since long. We aimed to investigate whether Ziziphus spina-christi leaf extract (ZSCLE) exerted a myocardial and renal protective effect on mice in which sepsis had been induced with cecal ligation and puncture (CLP). Male C57BL/6 mice were divided randomly into six groups (n = 7): sham-operated group, sham-operated mice treated with ZSCLE (300 mg/kg), CLP-induced sepsis group, ZSCLE (100 mg/kg)-treated group, ZSCLE (200 mg/kg)-treated group, and ZSCLE (300 mg/kg)-treated group...
March 14, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
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