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cecal ligation and. puncture

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https://www.readbyqxmd.com/read/29240643/temporally-distinct-regulation-of-pathways-contributing-to-cardiac-proteostasis-during-the-acute-and-recovery-phases-of-sepsis
#1
Kristen T Crowell, Samantha Moreno, Jennifer L Steiner, Catherine S Coleman, David I Soybel, Charles H Lang
BACKGROUND: Cardiac dysfunction is a common manifestation of sepsis and is associated with early increases in inflammation and decreases in myocardial protein synthesis. However, little is known regarding the molecular mechanisms regulating protein homeostasis during the recovery phase after the removal of the septic nidus. Therefore, the purpose of this study was to investigate diverse signal transduction pathways that regulate myocardial protein synthesis and degradation. METHODS: Adult male C57BL/6 mice were used to identify potential mechanisms mediating the acute (24 h) effect of cecal ligation and puncture (CLP) as well as long-term changes that manifest during the chronic (10 d) recovery phase...
December 13, 2017: Shock
https://www.readbyqxmd.com/read/29239329/activation-of-the-akt-mtor-signaling-pathway-a-potential-response-to-long-term-neuronal-loss-in-the-hippocampus-after-sepsis
#2
Jia-Nan Guo, Lin-Yu Tian, Wen-Yu Liu, Jie Mu, Dong Zhou
Survivors of sepsis may suffer chronic cognitive impairment as a long-term sequela. However, the precise mechanisms of cognitive dysfunction after sepsis are not well understood. We employed the cecal ligation-and-puncture-induced septic mouse model. We observed elevated phosphorylation of Akt, mammalian target of rapamycin (mTOR) and p70S6K on days 14 and 60, progressive neuronal loss in the cornu ammonis 1 region, and abnormal neuronal morphology in the hippocampus in the sepsis mouse model. These findings indicate that changes in neuronal morphology and number in the hippocampus after sepsis were associated with strong activation of the Akt/mTOR signaling pathway, and may reflect a "self-rescuing" feedback response to neuronal loss after sepsis...
November 2017: Neural Regeneration Research
https://www.readbyqxmd.com/read/29232157/bacterial-dissemination-to-the-brain-in-sepsis
#3
Benjamin H Singer, Robert P Dickson, Scott J Denstaedt, Michael W Newstead, Kwi Kim, Nicole R Falkowski, John R Erb-Downward, Thomas M Schmidt, Gary B Huffnagle, Theodore J Standiford
RATIONALE: Sepsis causes both brain dysfunction and neuroinflammation. It is unknown whether neuroinflammation in sepsis is initiated by dissemination of bacteria to the brain and sustained by persistent infection, or whether neuroinflammation is a sterile process resulting solely from circulating inflammatory mediators. OBJECTIVES: To determine if gut bacteria translocate to the brain during sepsis, and are associated with neuroinflammation. METHODS: Murine sepsis was induced using cecal ligation and puncture and sepsis survivor mice were compared to sham and unoperated controls...
December 12, 2017: American Journal of Respiratory and Critical Care Medicine
https://www.readbyqxmd.com/read/29229127/platonin-mitigates-vascular-hyporeactivity-of-thoracic-aorta-in-septic-rats
#4
Tsui-Chin Peng, Chao-Yuan Chang, I-Tao Huang, Ming-Chang Kao, Ya-Ying Chang, Chun-Jen Huang
BACKGROUND: Vascular hyporeactivity contributes to hemodynamic alterations and circulatory failure in severe sepsis. Among the identified mechanisms, inflammation and oxidative stress are the most crucial ones in mediating the development of vascular hyporeactivity induced by sepsis. Platonin, a photosensitive dye and an antioxidant, possesses potent antiinflammation effects. We elucidated whether platonin could mitigate vascular hyporeactivity of thoracic aorta in septic rats. MATERIAL AND METHODS: Adult male Sprague-Dawley rats were randomized to receive sham operation (Sham), Sham plus platonin (100 μg/kg), cecal ligation and puncture (CLP), or CLP plus platonin (10, 50, or 100 μg/kg) and designated as the Sham, P, CLP, CLP + P(10), CLP + P(50), and CLP + P(100) group, respectively (n = 6 in each group)...
January 2018: Journal of Surgical Research
https://www.readbyqxmd.com/read/29228667/metformin-ameliorates-sepsis-induced-brain-injury-by-inhibiting-apoptosis-oxidative-stress-and-neuroinflammation-via-the-pi3k-akt-signaling-pathway
#5
Guangming Tang, Huiyun Yang, Jing Chen, Mengrao Shi, Lingqing Ge, Xuhua Ge, Guoji Zhu
Sepsis-induced brain injuries increase mortality, morbidity, cognitive impairment and lack of effective therapeutic treatment. Previous studies have suggested that metformin provides neuroprotective effects against ischemia, brain trauma and other brain damage, but whether metformin protects a septic brain remains unknown. Thus, the aim of this study is to investigate the possible effects and the mechanism of metformin against septic brain damage using the cecal ligation and puncture (CLP) model. Mice were randomly divided into five groups: the Sham group, CLP group, CLP+ Met group, CLP+ vehicle group and CLP+ Met+ LY group...
November 17, 2017: Oncotarget
https://www.readbyqxmd.com/read/29227823/lncrna-malat1-regulates-sepsis-induced-cardiac-inflammation-and-dysfunction-via-interaction-with-mir-125b-and-p38-mapk-nf%C3%AE%C2%BAb
#6
Hu Chen, Xiaoyun Wang, Xuetao Yan, Xiaoli Cheng, Xianghu He, Wenzhong Zheng
This study aimed to explore the role of MALAT1 in sepsis-induced cardiac inflammation and dysfunction. We constructed the rat sepsis models through cecal ligation and puncture (CLP). The cardiac function, including left ventricular peak pressure (LVPP), left ventricular end diastolic pressure (LVEDP) and maximum rate of rise/fall of left ventricle pressure (±dp/dtmax) as well as the contents of serum cardiac troponin I (CTn-I), creatine kinase (CK), and creative kinase isoenzyme MB (CK-MB) were detected after modeling...
December 7, 2017: International Immunopharmacology
https://www.readbyqxmd.com/read/29222696/thrombomodulin-favors-leukocyte-microvesicle-fibrinolytic-activity-reduces-netosis-and-prevents-septic-shock-induced-coagulopathy-in-rats
#7
Julie Helms, Raphaël Clere-Jehl, Elsa Bianchini, Pierrick Le Borgne, Mélanie Burban, Fatiha Zobairi, Jean-Luc Diehl, Lelia Grunebaum, Florence Toti, Ferhat Meziani, Delphine Borgel
BACKGROUND: Septic shock-induced disseminated intravascular coagulation is responsible for increased occurrence of multiple organ dysfunction and mortality. Immunothrombosis-induced coagulopathy may contribute to hypercoagulability. We aimed at determining whether recombinant human thrombomodulin (rhTM) could control exaggerated immunothrombosis by studying procoagulant responses, fibrinolysis activity borne by microvesicles (MVs) and NETosis in septic shock. METHODS: In a septic shock model after a cecal ligation and puncture-induced peritonitis (H0), rats were treated with rhTM or a placebo at H18, resuscitated and monitored during 4 h...
December 8, 2017: Annals of Intensive Care
https://www.readbyqxmd.com/read/29218051/sphingosine-1-phosphate-and-c-c-chemokine-receptor-2-dependent-activation-of-cd4-plasmacytoid-dendritic-cells-in-the-bone-marrow-contributes-to-signs-of-sepsis-induced-immunosuppression
#8
Anna Smirnov, Stephanie Pohlmann, Melanie Nehring, Shafaqat Ali, Ritu Mann-Nüttel, Stefanie Scheu, Anne-Charlotte Antoni, Wiebke Hansen, Manuela Büettner, Miriam J Gardiasch, Astrid M Westendorf, Florian Wirsdörfer, Eva Pastille, Marcel Dudda, Stefanie B Flohé
Sepsis is the dysregulated response of the host to systemic, mostly bacterial infection, and is associated with an enhanced susceptibility to life-threatening opportunistic infections. During polymicrobial sepsis, dendritic cells (DCs) secrete enhanced levels of interleukin (IL) 10 due to an altered differentiation in the bone marrow and contribute to the development of immunosuppression. We investigated the origin of the altered DC differentiation using murine cecal ligation and puncture (CLP), a model for human polymicrobial sepsis...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/29216941/-impact-of-unfractionated-heparin-on-serum-and-liver-tissue-expression-of-heparanase-in-the-liver-injury-of-mice-with-sepsis
#9
Ziwei Hu, Xiaojuan Zhang, Song Chen, Ran Zhu, Xiaochun Ma
OBJECTIVE: To investigate the effect of unfractionated heparin on the expression of serum and liver tissue heparanase (HPA) in mice with liver injury induced by sepsis. METHODS: Forty-eight healthy male C57BL/6 mice aged 6-8 weeks were divided into groups according to random number table method. Twenty-four septic mice models (CLP group) were established by cecal ligation and puncture (CLP); the other 24 mice underwent sham operation (sham group), only laparotomy and abdominal closure were performed without ligation...
December 2017: Zhonghua Wei Zhong Bing Ji Jiu Yi Xue
https://www.readbyqxmd.com/read/29207668/genetic-or-pharmacologic-inhibition-of-egfr-ameliorates-sepsis-induced-aki
#10
Xuan Xu, Juan Wang, Ruhao Yang, Zheng Dong, Dongshan Zhang
Despite recent studies have demonstrated that the EGF receptor (EGFR) activation provided a renoprotective role during ischemic and folic acid-induced AKI, the role and regulation mechanism of EGFR in septic AKI remains unclear. Here, gefitinib, a highly selective EGFR inhibitor, abrogated LPS-induced phosphorylation of EGFR, ERK1/2, and STAT3 as well as expression of COX, eNOS, and proinflammatory cytokines in HK-2 cells. In addition, c-Src is an upstream of EGFR signaling pathway and mediates LPS-induced EGFR transactivation...
October 31, 2017: Oncotarget
https://www.readbyqxmd.com/read/29206760/development-of-noninvasive-in-vivo-approach-to-assess-vascular-permeability-in-inflammation-using-fluorescence-imaging
#11
Takashi Shimazui, Taka-Aki Nakada, Lisa Fujimura, Akemi Sakamoto, Masahiko Hatano, Shigeto Oda
INTRODUCTION: In vivo fluorescence imaging can quantify vascular permeability without requiring sacrifice of animals. However, use of this noninvasive approach for vascular permeability assessment in remote organ injury caused by systemic inflammatory disease has not been reported. METHODS: Evans blue (EB) and Genhance 750 fluorescent dye were mixed and injected into mice. The lung as a remote organ and the footpad as a noninvasive observational site were assessed in a cecal ligation and puncture (CLP)-induced systemic inflammation mouse model and compared with sham and hydrocortisone pretreated (CLP + HC) mouse models...
December 4, 2017: Shock
https://www.readbyqxmd.com/read/29204105/treatment-with-24%C3%A2-h-delayed-normo-and-hyperbaric-oxygenation-in-severe-sepsis-induced-by-cecal-ligation-and-puncture-in-rats
#12
Nina Falcon Bærnthsen, Marco Bo Hansen, Anna Mygind Wahl, Ulf Simonsen, Ole Hyldegaard
Background: Septic shock remains a leading cause of death worldwide. Hyperbaric oxygen treatment (HBO2) has been shown to alter the inflammatory response during sepsis and to reduce mortality. A therapeutic window of HBO2 treatment has been demonstrated experimentally, but optimal timing remains uncertain. We investigated the effects of 24 h delayed normobaric oxygen (NBO2) and HBO2 treatment on the endogenous production of the inflammatory markers interleukin (IL)-6, tumor necrosis factor (TNF)-α and IL-10, and on mortality in rats with cecal ligation and puncture (CLP) induced sepsis...
2017: Journal of Inflammation
https://www.readbyqxmd.com/read/29201213/protective-role-of-tenuigenin-on-sepsis-induced-acute-kidney-injury-in-mice
#13
Yan Xu, Yan Jiang, Wentao Gai, Botao Yu
Acute kidney injury (AKI) is a severe complication of sepsis, which largely contributes to the associated high mortality rate. Tenuigenin (TNG) is a natural product isolated from Polygala tenuifolia root, which possesses anti-inflammatory and anti-oxidant properties. The present study investigated the effects of TNG on sepsis-associated AKI in mice subjected to cecal ligation and puncture (CLP). TNG was demonstrated to alleviate sepsis-induced AKI by reducing pathological changes and significantly decreasing the levels of blood urea nitrogen, serum creatinine and kidney coefficient...
November 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/29201164/electroacupuncture-improves-acute-bowel-injury-recovery-in-rat-models
#14
Jiannong Wu, Bin Lyu, Tie'er Gan, Lingcong Wang, Meifei Zhu
Electroacupuncture (EA) accelerates intestinal functional recovery in sepsis. The present study investigated ghrelin and ghrelin receptor (GSH-R) levels during EA in rats with acute bowel injury (ABI). Rats were grouped into four groups: Sham, ABI, ABI+EA and ABI+GHRA+EA (n=12 per group). ABI was induced by cecal ligation and puncture (CLP). EA on bilateral Zusanli acupoints was performed following CLP. GSH-R blocker (GHRA) was used following CLP but prior to EA for ABI+GHRA+EA rats. Rats were sacrificed 12 h following CLP...
November 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/29198013/short-and-long-term-protective-effects-of-melatonin-in-a-mouse-model-of-sepsis-associated-encephalopathy
#15
Mu-Huo Ji, De-Guo Xia, Lan-Yue Zhu, Xia Zhu, Xiao-Yan Zhou, Jiang-Yan Xia, Jian-Jun Yang
Brain dysfunction is a common complication after sepsis and is an independent risk factor for a poor prognosis, which is partly attributed to the dysregulated inflammatory response and oxidative damage. Melatonin regulates the sleep-wake cycle and also has potent anti-inflammatory and antioxidant properties, yet the protective effects of melatonin on sepsis-induced neurobehavioral dysfunction remain to be elucidated. In the present study, melatonin was administered intraperitoneally daily at a dose of 10 mg/kg for three consecutive days immediately (early treatment) or 7 days (delayed treatment) after sham operation or cecal ligation and puncture (CLP), followed by an additional treatment in drinking water until the end of behavioral tests...
December 2, 2017: Inflammation
https://www.readbyqxmd.com/read/29196339/intra-abdominal-infection-combined-with-intra-abdominal-hypertension-aggravates-the-intestinal-mucosal-barrier-dysfunction
#16
Yuan Li, Jianan Ren, Xiuwen Wu, Jieshou Li
Some patients with intra-abdominal infection (IAI) may develop intra-abdominal hypertension (IAH) during treatment. This study investigated the impact of IAI combined with IAH on the intestinal mucosal barrier in a rabbit model. Forty-eight New Zealand white rabbits were randomly divided into four groups: (1) IAI & IAH; (2) IAI alone; (3) IAH alone; and (4) Control group. IAI model: cecal ligation and puncture for 48 hours; IAH model: raised intra-abdominal pressure of 20 mmHg for 4 hours. Pathological changes of intestinal mucosa were confirmed by light and scanning electron microscopy...
December 1, 2017: Bioscience Reports
https://www.readbyqxmd.com/read/29180214/thioredoxin-1-attenuates-sepsis-induced-cardiomyopathy-after-cecal-ligation-and-puncture-in-mice
#17
Rickesha L Wilson, Vaithinathan Selvaraju, Rajesh Lakshmanan, Mahesh Thirunavukkarasu, Jacob Campbell, David W McFadden, Nilanjana Maulik
BACKGROUND: Sepsis is a leading cause of mortality among patients in intensive care units across the USA. Thioredoxin-1 (Trx-1) is an essential 12 kDa cytosolic protein that, apart from maintaining the cellular redox state, possesses multifunctional properties. In this study, we explored the possibility of controlling adverse myocardial depression by overexpression of Trx-1 in a mouse model of severe sepsis. METHODS: Adult C57BL/6J and Trx-1Tg/+ mice were divided into wild-type sham (WTS), wild-type cecal ligation and puncture (WTCLP), Trx-1Tg/+sham (Trx-1Tg/+S), and Trx-1Tg/+CLP groups...
December 2017: Journal of Surgical Research
https://www.readbyqxmd.com/read/29180187/glutamine-reduces-myocardial-cell-apoptosis-in-a-rat-model-of-sepsis-by-promoting-expression-of-heat-shock-protein-90
#18
Wanxia Li, Shaoyu Tao, Qinghua Wu, Tao Wu, Ran Tao, Jun Fan
BACKGROUND: Myocardial cell injury and cardiac myocyte apoptosis are associated with sepsis. Glutamine (Gln) has been reported to repair myocardial cell injury. The aim of this study was to explore the role of Gln on cardiac myocytes in a cecal ligation and puncture (CLP) model of sepsis in Wistar rats. MATERIALS AND METHODS: Following induction of sepsis in a CLP rat model, viral encoding heat shock protein 90 (Hsp90) gene and Hsp90dsDNA were designed to express and knockdown Hsp90, respectively...
December 2017: Journal of Surgical Research
https://www.readbyqxmd.com/read/29170136/cereblon-deficiency-confers-resistance-against-polymicrobial-sepsis-by-the-activation-of-amp-activated-protein-kinase-and-heme-oxygenase-1
#19
Minchan Gil, Yun Kyu Kim, Ha Yeong Kim, Hyo-Kyung Pak, Chan-Sik Park, Kyung Jin Lee
Cereblon (CRBN) has a pleiotropic role in important cellular processes and is a potential therapeutic target in several diseases, including mental retardation, cancer, and metabolic disorders. The role of CRBN in polymicrobial sepsis induced by cecal ligation and puncture (CLP) was investigated using CRBN-deficient (KO) mice. Survival following CLP was significantly higher in KO mice compared to wild-type (WT) controls (50% vs 0% at day 6 after CLP). The improved survival of KO mice was accompanied by reduced peripheral blood bacterial load and lung injury...
November 20, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29152323/new-melanocortin-like-peptide-of-e-coli-can-suppress-inflammation-via-the-mammalian-melanocortin-1-receptor-mc1r-possible-endocrine-like-function-for-microbes-of-the-gut
#20
Xiaoling Qiang, Anthony S Liotta, Joseph Shiloach, J C Gutierrez, Haichao Wang, Mahendar Ochani, Kanta Ochani, Huan Yang, Aviva Rabin, Derek LeRoith, Maxine A Lesniak, Markus Böhm, Christian Maaser, Klaus Kannengiesser, Mark Donowitz, Shervin Rabizadeh, Christopher J Czura, Kevin J Tracey, Mark Westlake, Aida Zarfeshani, Syed F Mehdi, Ann Danoff, Xueliang Ge, Suparna Sanyal, Gary J Schwartz, Jesse Roth
E. coli releases a 33 amino acid peptide melanocortin-like peptide of E. coli (MECO-1) that is identical to the C-terminus of the E. coli elongation factor-G (EF-G) and has interesting similarities to two prominent mammalian melanocortin hormones, alpha-melanocyte-stimulating hormone (alpha-MSH) and adrenocorticotropin (ACTH). Note that MECO-1 lacks HFRW, the common pharmacophore of the known mammalian melanocortin peptides. MECO-1 and the two hormones were equally effective in severely blunting release of cytokines (HMGB1 and TNF) from macrophage-like cells in response to (i) endotoxin (lipopolysaccharide) or (ii) pro-inflammatory cytokine HMGB-1...
2017: NPJ Biofilms and Microbiomes
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