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https://www.readbyqxmd.com/read/28918363/changes-in-zn-homeostasis-during-long-term-culture-of-primary-endothelial-cells-and-effects-of-zn-on-endothelial-cell-senescence
#1
Marco Malavolta, Laura Costarelli, Robertina Giacconi, Andrea Basso, Francesco Piacenza, Elisa Pierpaoli, Mauro Provinciali, Ogo A Ogo, Dianne Ford
Endothelial cell senescence and Zn nutritional status influence cardiovascular disease. The influence of Zn appears dichotomous, hence it is imperative to understand the relationship with cellular senescence to improve knowledge about the molecular and cellular basis of the disease. Here we aimed to determine: 1) the impact of chronic exposure to a moderately high dose of Zn on senescence of endothelial cells; 2) the changes in Zn homeostasis during the lifespan of primary cultured endothelial cells; and 3) the susceptibility of proliferating and senescent endothelial cells to cell death after short term exposure to increasing doses of Zn and of the Zn chelator TPEN...
September 13, 2017: Experimental Gerontology
https://www.readbyqxmd.com/read/28915963/hepcidin-and-metallothioneins-as-molecular-base-for-sex-dependent-differences-in-clinical-course-of-experimental-autoimmune-encephalomyelitis-in-chronic-iron-overload
#2
Božena Ćurko-Cofek, Tanja Grubić Kezele, Vesna Barac-Latas
Multiple sclerosis is a chronic demyelinating disease of the central nervous system characterised by inflammatory and degenerative changes. It is considered that disease arises from the influence of environmental factors on genetically susceptible individuals. Recent researches, using magnetic resonance imaging, connected iron deposits in different brain regions with demyelinating process in multiple sclerosis patients. Although iron is an essential trace element important for many biological functions it could be harmful because iron excess can induce the production of reactive oxygen species, development of oxidative stress and lipid peroxidation which leads to demyelination...
September 2017: Medical Hypotheses
https://www.readbyqxmd.com/read/28913608/efficacy-of-%C3%AE-lipoic-acid-against-cadmium-toxicity-on-metal-ion-and-oxidative-imbalance-and-expression-of-metallothionein-and-antioxidant-genes-in-rabbit-brain
#3
Hamida M Saleh, Yasser S El-Sayed, Sherif M Naser, Abdelgawad S Eltahawy, Atsuto Onoda, Masakazu Umezawa
To explore the protective efficacy of α-lipoic acid (ALA) against Cd-prompted neurotoxicity, young male New Zealand rabbits (Oryctolagus cuniculus) were divided randomly into four groups. Group 1 (control) received demineralized water. Group 2 (Cd) administered cadmium chloride (CdCl2) 3 mg/kg bwt. Group 3 (ALA) administered ALA 100 mg/kg bwt. Group 4 (Cd + ALA) administered ALA 1 h after Cd. The treatments were administered orally for 30 consecutive days. Cd-induced marked disturbances in neurochemical parameters were indicated by the reduction in micro- and macro-elements (Zn, Fe, Cu, P, and Ca), with the highest reduction in Cd-exposed rabbits, followed by Cd + ALA group and then ALA group...
September 14, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28913548/changes-in-histopathology-enzyme-activities-and-the-expression-of-relevant-genes-in-zebrafish-danio-rerio-following-long-term-exposure-to-environmental-levels-of-thallium
#4
Li-Ping Hou, Yang Yang, Hu Shu, Guang-Guo Ying, Jian-Liang Zhao, Yi-Bing Chen, Yong-Heng Chen, Gui-Zhen Fang, Xin Li, Ji-Sheng Liu
Thallium is a rare-earth element, but widely distributed in water environments, posing a potential risk to our health. This study was designed to investigate the chronic effects of thallium based on physiological responses, gene expression, and changes in the activity of relevant enzymes in adult zebra fish exposed to thallium at low doses. The endpoints assessed include mRNA expression of metallothionein (MT)2 and heat shock protein HSP70; enzymatic activities of superoxide dismutase (SOD) and Na(+)/K(+)-ATPase; and the histopathology of gill, gonad, and liver tissues...
September 14, 2017: Bulletin of Environmental Contamination and Toxicology
https://www.readbyqxmd.com/read/28898278/transcriptome-analysis-of-phytolacca-americana-l-in-response-to-cadmium-stress
#5
Yongkun Chen, Junkai Zhi, Hao Zhang, Jian Li, Qihong Zhao, Jichen Xu
Phytolacca americana L. (pokeweed) has metal phytoremediation potential, but little is known about its metal accumulation-related genes. In this study, the de novo sequencing of total RNA produced 53.15 million reads covering 10.63 gigabases of transcriptome raw data in cadmium (Cd)-treated and untreated pokeweed. Of the 97,502 assembled unigenes, 42,197 had significant matches in a public database and were annotated accordingly. An expression level comparison between the samples revealed 1515 differentially expressed genes (DEGs), 923 down- and 592 up-regulated under Cd treatment...
2017: PloS One
https://www.readbyqxmd.com/read/28875714/effects-of-co-exposure-to-imidacloprid-and-gibberellic-acid-on-redox-status-kidney-variables-and-histopathology-in-adult-rats
#6
Bornia Lafi, Mariem Chaâbane, Awatef Elwej, Malek Grati, Kamel Jamoussi, Hela Mnif, Tahia Boudawara, Hanen Ketata Bouaziz, Najiba Zeghal
Data on the individual nephrotoxic effects of imidacloprid (IMI) and gibberellic acid (GA3) are scarce. Moreover, there is a lack of information about their combined effects on the renal tissue. Our study investigated the effects of IMI and GA3 separately or together on rats kidney. IMI (64 mg/kg bw) was given for 3 weeks by gavage either individually or in combination with GA3 (200 mg/L) via drinking water. IMI associated or no with GA3 increased the levels of kidney malondialdehyde, advanced oxidation protein products, protein carbonyls and metallothionein, plasma creatinine, urea, blood urea nitrogen and lactate dehydrogenase activity...
September 6, 2017: Archives of Physiology and Biochemistry
https://www.readbyqxmd.com/read/28870952/metallothionein-isoform-expression-in-benign-and-malignant-thyroid-lesions
#7
Beata Wojtczak, Bartosz Pula, Agnieszka Gomulkiewicz, Mateusz Olbromski, Marzena Podhorska-Okolow, Paweł Domoslawski, Marek Bolanowski, Jacek Daroszewski, Piotr Dziegiel
BACKGROUND: Metallothioneins (MTs) are involved in numerous cell processes such as binding and transport of zinc and copper ions, differentiation, proliferation and apoptosis, therefore contributing to carcinogenesis. Scarce data exist on their expression in benign and malignant lesions of the thyroid. MATERIALS AND METHODS: mRNA expression of functional isoforms of MT genes (MT1A, MT1B, MT1E, MT1F, MT1G, MT1H, MT1X, MT2A, MT4) was studied in 17 nodular goiters (NG), 12 follicular adenomas (FA) and 26 papillary thyroid carcinomas (PTC)...
September 2017: Anticancer Research
https://www.readbyqxmd.com/read/28866860/protective-effects-of-dietary-garlic-powder-against-cadmium-induced-toxicity-in-sea-bass-liver-a-chemical-biochemical-and-transcriptomic-approach
#8
Amin Mosbah, Hamadi Guerbej, Hamadi Boussetta, Zied Bouraoui, Mohamed Banni
To investigate the protective effect of garlic powder on cadmium-induced toxicity sea bass liver, juvenile fishes where maintained under three food diets (diet 1: normal without garlic supply, diet 2: 2% garlic powder; diet 3: 6% garlic powder). After 30 days of specific diets, each group was injected with 500 μg kg(-1)of Cd. The control group was the one fed with normal diet and not injected with Cd. Liver Cd, Zn, and Se loads was assessed after 1 and 3 days of Cd injections. Moreover, antioxidant enzymes activities termed as catalase, superoxide dismutase, and glutathione peroxydase as well as their gene expression levels were monitored...
September 2, 2017: Biological Trace Element Research
https://www.readbyqxmd.com/read/28865957/zinc-binds-non-cooperatively-to-human-liver-metallothionein-2a-at-physiological-ph
#9
Devika P Jayawardena, Ilka U Heinemann, Martin J Stillman
Maintenance of the homeostasis of zinc is very important in regulating bodily functions. There are over 300 Zn-dependent enzymes identified where Zn(II) plays a structural or catalytic role. However, an excess of Zn(II) in a cell is toxic and free Zn(II) is tightly controlled. Metallothioneins (MTs) are small cysteine rich proteins that can bind up to seven Zn(II) and act as a Zn(II) reservoir. The MT2a isoform is predominantly found in the liver. This study focused on designing an MT2a construct of recombinant human MT2a to determine the Zn(II) binding profile of MT2a in vitro...
August 30, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28852914/induction-of-metallothionein-in-rat-liver-by-zinc-exposure-a-dose-and-time-dependent-study
#10
Roobee Garla, Preeti Kango, Navneet Kaur Gill, M L Garg
Metallothioneins (MTs) are low molecular weight ubiquitous metalloproteins with high cysteine (thiol) content. The intracellular concentration of zinc (Zn) is tightly regulated and MT plays a crucial role in it. The present study investigates the relationship between the Zn status (as a function of Zn concentration and time) in the rat liver and the occurrence of hepatic MT. For dose dependent study, four experimental groups, one control and three receiving different levels of metal supplementation, were chosen [Group 1 control and Group 2, Group 3, Group 4 receiving subcutaneous dose of 10, 50 and 100 mg of Zn/kg body weight (in the form of ZnSO4·7H2O), respectively]...
August 29, 2017: Protein Journal
https://www.readbyqxmd.com/read/28845600/implications-of-impaired-zinc-homeostasis-in-diabetic-cardiomyopathy-and-nephropathy
#11
REVIEW
Robertina Giacconi, Lu Cai, Laura Costarelli, Maurizio Cardelli, Marco Malavolta, Francesco Piacenza, Mauro Provinciali
Impaired zinc homeostasis is observed in diabetes mellitus (DM2) and its complications. Zinc has a specific role in pancreatic β-cells via insulin synthesis, storage, and secretion. Intracellular zinc homeostasis is tightly controlled by zinc transporters (ZnT and Zip families) and metallothioneins (MT) which modulate the uptake, storage, and distribution of zinc. Several investigations in animal models demonstrate the protective role of MT in DM2 and its cardiovascular or renal complications, while a copious literature shows that a common polymorphism (R325W) in ZnT8, which affects the protein's zinc transport activity, is associated with increased DM2 risk...
August 27, 2017: BioFactors
https://www.readbyqxmd.com/read/28833895/morphostructural-and-immunohistochemical-study-on-the-role-of-metallothionein-in-the-detoxification-of-heavy-metals-in-apis-mellifera-l-1758
#12
Antonio Salvaggio, Roberta Pecoraro, Elena Maria Scalisi, Daniele Tibullo, Bianca Maria Lombardo, Giuseppina Messina, Francesco Loreto, Chiara Copat, Margherita Ferrante, Roberto Avola, Giusi D'amante, Claudia Genovese, Salvatore Antonino Raccuia, Maria Violetta Brundo
Honey is a valuable food produced by bees from sugary substances that they gather in nature. The transformation the nectar into honey, by bees, is long and complex. Except for honey, where heavy metals are absent or are found only in traces, the bees and their products have always been considered excellent biomarkers of such contaminants. We have assumed that the absence of heavy metals in honey is due to the presence of a detoxification system in the digestive system of bees, which involves metallothioneins, proteins that have a role in the homeostatic control of essential and non-essential metals...
August 22, 2017: Microscopy Research and Technique
https://www.readbyqxmd.com/read/28829377/successive-onset-of-molecular-cellular-and-tissue-specific-responses-in-midgut-gland-of-littorina-littorea-exposed-to-sub-lethal-cadmium-concentrations
#13
Denis Benito, Michael Niederwanger, Urtzi Izagirre, Reinhard Dallinger, Manu Soto
Cadmium (Cd) is one of the most harmful metals, being toxic to most animal species, including marine invertebrates. Among marine gastropods, the periwinkle (Littorina littorea) in particular can accumulate high amounts of Cd in its midgut gland. In this organ, the metal can elicit extensive cytological and tissue-specific alterations that may reach, depending on the intensity of Cd exposure, from reversible lesions to pathological cellular disruptions. At the same time, Littorina littorea expresses a Cd-specific metallothionein (MT) that, due to its molecular features, expectedly exerts a protective function against the adverse intracellular effects of this metal...
August 22, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28822934/ecotoxicological-effects-of-binary-mixtures-of-siduron-and-cd-on-mrna-expression-in-the-earthworm-eisenia-fetida
#14
Herman Uwizeyimana, Meie Wang, Weiping Chen, Kifayatullah Khan
This study aimed to investigate the eco-toxicological responses of earthworm (Eisenia fetida) exposed to combined siduron (herbicide) and cadmium (Cd). Eisenia fetida gene expressions including metallothionein (MT) and heat shock protein70 (Hsp70) were analyzed using real-time Polymerase Chain Reaction after individual and combined siduron (0.90, 1.80, 3.60 and 7.20μgcm(-2)) and Cd (0.225, 0.45, 0.90 and 1.80μgcm(-2)) sublethal exposures. Where, the nature of the toxicological interactions between siduron and Cd in inducing or suppressing MT and Hsp70 expression was determined by applying the Combination index (CI)-isobologram model...
August 17, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28812260/zinc-metabolism-and-metallothioneins
#15
REVIEW
Abdulkerim Kasim Baltaci, Kemal Yuce, Rasim Mogulkoc
Among the trace elements, zinc is one of the most used elements in biological systems. Zinc is found in the structure of more than 2700 enzymes, including hydrolases, transferases, oxyreductases, ligases, isomerases, and lyases. Not surprisingly, it is present in almost all body cells. Preserving the stability and integrity of biological membranes and ion channels, zinc is also an intracellular regulator and provides structural support to proteins during molecular interactions. It acts as a structural element in nucleic acids or other gene-regulating proteins...
August 15, 2017: Biological Trace Element Research
https://www.readbyqxmd.com/read/28806554/metal-bioaccumulation-and-biomarkers-of-effects-in-caged-mussels-exposed-in-the-athabasca-oil-sands-area
#16
M Pilote, C André, P Turcotte, F Gagné, C Gagnon
The Athabasca oil sands deposit is the world's largest known reservoir of crude bitumen and the third-largest proven crude oil reserve. Mining activity is known to release contaminants, including metals, and to potentially impact the aquatic environment. The purpose of this study was to determine the impacts of oil sands mining on water quality and metal bioaccumulation in mussels from the Fort McMurray area in northern Alberta, Canada. The study presents two consecutive years of contrasting mussel exposure conditions (low and high flows)...
August 11, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28804566/microrna-93-inhibits-apoptosis-and-promotes-proliferation-invasion-and-migration-of-renal-cell-carcinoma-achn-cells-via-the-tgf-%C3%AE-smad-signaling-pathway-by-targeting-runx3
#17
Li-Jie Liu, Jian-Jun Yu, Xiao-Lin Xu
We investigated the ability of microRNA-93 (miR-93) to influence proliferation, invasion, migration, and apoptosisofrenal cell carcinoma (RCC) cells via transforming growth factor-β/solvated metal atom dispersed (TGF-β/Smad) signaling by targeting runt-related transcription factor 3 (RUNX3). RCC tissues with corresponding adjacent normal tissues were collected from 249 RCC patients. And normal renal tissues were collected from patients without RCC who received nephrectomy. The RCC cell line ACHN was treated with miR-93 mimic, mimic-negative control (NC), miR-93 inhibitor, inhibitor-NC, and miR-93 inhibitor + small interfering RNA (siRNA) against RUNX3 (si-RUNX3)...
2017: American Journal of Translational Research
https://www.readbyqxmd.com/read/28802572/effect-of-cadmium-on-uptake-of-iron-zinc-and-copper-and-mrna-expression-of-metallothioneins-in-hepg2-cells-in-vitro
#18
Sabine Sampels, Hana Kocour Kroupova, Pavla Linhartova
The intake of cadmium contaminated fish was mimicked by incubating human hepatoblastoma cells (Cell line HepG2) with a combination of different levels of cadmium (0-5μM) plus the n-3 fatty acids docosahexaenoic acid and eicosapentaenoic acid, which are typical for fish. Uptake of cadmium, iron, copper and zinc was measured by ICP-MS. In addition mRNA expression of two metallothioneins (mt1 g and mt1 m) was evaluated by real-time PCR. The obtained data shows that the presence of cadmium increases the uptake of iron and zinc into the HepG2 cells while the uptake of copper remains unaffected...
October 2017: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/28801265/fluoxetine-coupled-with-zinc-in-a-chronic-mild-stress-model-of-depression-providing-a-reservoir-for-optimum-zinc-signaling-and-neuronal-remodeling
#19
Nesreen Nabil Omar, Reham Fathy Tash
BACKGROUND: Recently, depression has been envisioned as more than an alteration in neurotransmitters centered around receptor signaling pathways. Consequently, the precise mechanisms of selective serotonin reuptake inhibitor (SSRI) antidepressant drugs such as fluoxetine are being revisited. Zinc is a trace element that has been long implicated in the psychopathology and therapy of depression. Zinc has been found to be sequestered and dispensed during stress and inflammation through a family of proteins called metallothioneins (MTs)...
August 9, 2017: Pharmacology, Biochemistry, and Behavior
https://www.readbyqxmd.com/read/28800079/challenging-the-metallothionein-mt-gene-of-biomphalaria-glabrata-unexpected-response-patterns-due-to-cadmium-exposure-and-temperature-stress
#20
Michael Niederwanger, Martin Dvorak, Raimund Schnegg, Veronika Pedrini-Martha, Katharina Bacher, Massimo Bidoli, Reinhard Dallinger
Metallothioneins (MTs) are low-molecular-mass, cysteine-rich, metal binding proteins. In most animal species, they are involved in metal homeostasis and detoxification, and provide protection from oxidative stress. Gastropod MTs are highly diversified, exhibiting unique features and adaptations like metal specificity and multiplications of their metal binding domains. Here, we show that the MT gene of Biomphalaria glabrata, one of the largest MT genes identified so far, is composed in a unique way. The encoding for an MT protein has a three-domain structure and a C-terminal, Cys-rich extension...
August 11, 2017: International Journal of Molecular Sciences
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