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https://www.readbyqxmd.com/read/28210877/quantum-mechanical-treatment-of-as-3-thiol-model-compounds-implication-for-the-core-structure-of-as-iii-metallothionein
#1
Roobee Garla, Narinder Kaur, Mohinder Pal Bansal, Mohan Lal Garg, Biraja Prasad Mohanty
Exposure to inorganic arsenic (As) is one of the major health concerns in several regions around the world. Binding of As(III) with thiols is central to the mechanisms related to its toxicity, detoxification, and therapeutic effects. Due to its high thiol content, metallothionein (MT) is presumed to play an important role in case of arsenic toxicity. Consequences of these As-thiol interactions are not yet clear due to various difficulties in the characterization of arsenic bound proteins by spectroscopic techniques...
March 2017: Journal of Molecular Modeling
https://www.readbyqxmd.com/read/28202635/short-term-subclinical-zinc-deficiency-in-weaned-piglets-affects-cardiac-redox-metabolism-and-zinc-concentration
#2
Daniel Brugger, Wilhelm M Windisch
Background: Subclinical zinc deficiency (SZD) represents the common zinc malnutrition phenotype. However, its association with oxidative stress is not well understood. The heart muscle may be a promising target for studying early changes in redox metabolism.Objective: We investigated the effects of short-term SZD on cardiac redox metabolism in weaned piglets.Methods: Forty-eight weaned German Large White × Landrace × Piétrain piglets (50% castrated males and 50% females; body weight of 8.5 kg) were fed diets with different zinc concentrations for 8 d...
February 15, 2017: Journal of Nutrition
https://www.readbyqxmd.com/read/28198206/nitraria-retusa-fruit-prevents-penconazole-induced-kidney-injury-in-adult-rats-through-modulation-of-oxidative-stress-and-histopathological-changes
#3
Mariem Chaâbane, Mohamed Koubaa, Nejla Soudani, Awatef Elwej, Malek Grati, Kamel Jamoussi, Tahia Boudawara, Semia Ellouze Chaabouni, Najiba Zeghal
CONTEXT: Nitraria retusa (Forssk.) Asch. (Nitrariaceae) is a medicinal plant which produces edible fruits whose antioxidant activity has been demonstrated. OBJECTIVE: The current study elucidates the potential protective effect of N. retusa fruit aqueous extract against nephrotoxicity induced by penconazole, a triazole fungicide, in the kidney of adult rats. MATERIALS AND METHODS: Adult Wistar rats were exposed either to penconazole (67 mg/kg body weight), or to N...
December 2017: Pharmaceutical Biology
https://www.readbyqxmd.com/read/28193588/variations-of-biomarkers-response-in-mussels-mytilus-galloprovincialis-to-low-moderate-and-high-concentrations-of-organic-chemicals-and-metals
#4
Lorena Perić, Vedrana Nerlović, Paula Žurga, Luka Žilić, Andreja Ramšak
The changes of acetylcholinesterase activity (AChE), metallothioneins content (MTs), catalase activity (CAT) and lipid peroxidation (LPO) were assessed after 4 days exposure of mussels Mytilus galloprovincialis to a wide range of sublethal concentrations of chlorpyrifos (CHP, 0.03-100 μg/L), benzo(a)pyrene (B(a)P, 0.01-100 μg/L), cadmium (Cd, 0.2-200 μg/L) and copper (Cu, 0.2-100 μg/L). The activity of AChE in the gills decreased after exposure to CHP and Cu, whereas no change of activity was detected after exposure to B(a)P and Cd...
January 31, 2017: Chemosphere
https://www.readbyqxmd.com/read/28193328/engineering-neurospora-crassa-for-cellobionate-production-directly-from-cellulose-without-any-enzyme-addition
#5
Hui Lin, Amanda Hildebrand, Takao Kasuga, Zhiliang Fan
In a cellulosic biorefinery, the cellulase enzymes needed for hydrolysis are one of the major contributors to high processing costs, while the hydrolysis product, cellobiose, has strong inhibition to the cellulases. In this study, we report engineering recombinant Neurospora crassa strains which are able to produce cellobionate, an organic acid, from cellulose without any enzyme addition. Recombinant strains were constructed by heterologously expressing laccase genes from different sources under different promoters in N...
April 2017: Enzyme and Microbial Technology
https://www.readbyqxmd.com/read/28187517/selective-cysteine-modification-of-metal-free-human-metallothionein-1a-and-its-isolated-domain-fragments-solution-structural-properties-revealed-via-esi-ms
#6
Gordon W Irvine, Melissa Santolini, Martin J Stillman
Human metallothionein 1a, a protein with two cysteine-rich metal-binding domains (α with 11 Cys and β with 9), was analyzed in its metal-free form by selective, covalent Cys modification coupled with ESI-MS techniques. The modification profiles of the isolated β- and α-fragments reacted with p-benzoquinone (Bq), N-ethylmalemide (NEM) and iodoacetamide (IAM) were compared with the full length protein using ESI-mass spectral data to follow the reaction pathway. Under denaturing conditions at low pH, the reaction profile with each modifier followed pathways that resulted in stochastic, Normal distributions of species whose maxima was equal to the mol...
February 10, 2017: Protein Science: a Publication of the Protein Society
https://www.readbyqxmd.com/read/28183007/thioredoxin-and-metallothionein-homeostasis-related-proteins-in-lip-carcinogenesis
#7
Patrícia C Caldeira, Laíssa S Silva, Aline C Batista, Maria Cássia F Aguiar
OBJECTIVE: Thioredoxin (Trx) and metallothionein (MT) are involved in the development of some carcinomas; however, the role of these proteins in labial carcinogenesis has not yet been tested. The aims of the study were to evaluate and to correlate the immunoexpression of Trx and MT in actinic cheilitis, lip squamous cell carcinoma, and normal vermillion lip mucosa. DESIGN: Immunohistochemistry was undertaken for Trx and MT in samples of actinic cheilitis, lip squamous cell carcinoma, and normal lip mucosa...
February 1, 2017: Archives of Oral Biology
https://www.readbyqxmd.com/read/28177898/maternal-dietary-zinc-supplementation-enhances-the-epigenetic-activated-antioxidant-ability-of-chick-embryos-from-maternal-normal-and-high-temperatures
#8
Yongwen Zhu, Xiudong Liao, Lin Lu, Wenxiang Li, Liyang Zhang, Cheng Ji, Xi Lin, Hsiao-Ching Liu, Jack Odle, Xugang Luo
The role of maternal dietary zinc supplementation in protecting the embryos from maternal hyperthermia-induced negative effects via epigenetic mechanisms was examined using an avian model (Gallus gallus). Broiler breeder hens were exposed to two maternal temperatures (21°C and 32°C) × three maternal dietary zinc treatments (zinc-unsupplemented control diet, the control diet + 110 mg zinc/kg inorganic or organic zinc) for 8 weeks. Maternal hyperthermia increased the embryonic mortality and induced oxidative damage evidenced by the elevated mRNA expressions of heat shock protein genes...
February 3, 2017: Oncotarget
https://www.readbyqxmd.com/read/28169244/expression-of-metallothionein-in-dimethylhydrazine-induced-colonic-precancerous-and-cancerous-model-in-rat
#9
Pamela Christudoss, Geeta Chacko, Ratnasamy Selvakumar, Jude Joseph Fleming, Srinivasan Pugazhendhi, George Mathew
AIM: Metallothionein (MT) is a small protein with a high affinity for divalent heavy metals and has a function in zinc homeostasis. The purpose of this study was to assess the MT mRNA gene expression as well as the MT protein content by immunohistochemistry and radioimmunoassay (RIA) in 1,2-dimethylhydrazine (DMH)-induced precancerous and cancerous colonic tissue in rats. MATERIALS AND METHODS: Six-week-old rats were given subcutaneous injections of DMH twice a week for 3 months and sacrificed at 4 months (precancerous model) and 6 months (cancerous model)...
October 2016: Journal of Cancer Research and Therapeutics
https://www.readbyqxmd.com/read/28158919/zinc-rescues-obesity-induced-cardiac-hypertrophy-via-stimulating-metallothionein-to-suppress-oxidative-stress-activated-bcl10-card9-p38-mapk-pathway
#10
Shudong Wang, Junlian Gu, Zheng Xu, Zhiguo Zhang, Tao Bai, Jianxiang Xu, Jun Cai, Gregory Barnes, Qiu-Ju Liu, Jonathan H Freedman, Yonggang Wang, Quan Liu, Yang Zheng, Lu Cai
Obesity often leads to obesity-related cardiac hypertrophy (ORCH), which is suppressed by zinc-induced inactivation of p38 mitogen-activated protein kinase (p38 MAPK). In this study, we investigated the mechanisms by which zinc inactivates p38 MAPK to prevent ORCH. Mice (4-week old) were fed either high fat diet (HFD, 60% kcal fat) or normal diet (ND, 10% kcal fat) containing variable amounts of zinc (deficiency, normal and supplement) for 3 and 6 months. P38 MAPK siRNA and the p38 MAPK inhibitor SB203580 were used to suppress p38 MAPK activity in vitro and in vivo, respectively...
February 3, 2017: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/28154959/differential-sexual-survival-of-drosophila-melanogaster-on-copper-sulfate
#11
Michael A Balinski, Ronny C Woodruff
Based on studies of the influence of X-chromosomes on the viability of Drosophila melanogaster exposed to cadmium, and on the role of X-linked genes on copper homeostasis, we examined the effect of copper sulfate (CuSO4) on offspring viability using three independent, inbred D. melanogaster crosses (ensuring identical autosomes for males and females within each cross). Each cross was performed with attached X-chromosome females and males with a single X-chromosome. As female D. melanogaster have less metallothionein RNA expression than males, we predicted fewer female offspring than male offspring in crosses exposed to CuSO4, even though females have two copies of X-chromosome genes, possibly resulting in overdominant heterozygosity...
February 2, 2017: Genetica
https://www.readbyqxmd.com/read/28152585/enhanced-metal-tolerance-correlates-with-heterotypic-variation-in-spmtl-a-metallothionein-like-protein-from-the-hyperaccumulator-sedum-plumbizincicola
#12
Jia-Shi Peng, Ge Ding, Shuan Meng, Hong-Ying Yi, Ji-Ming Gong
Mechanistic insight into metal hyperaccumulation is largely restricted to Brassicaceae plants, therefore it is of great importance to obtain corresponding knowledge from system outside the Brassicaceae. Here we constructed and screened a cDNA library of the Cd/Zn hyperaccumulator Sedum plumbizincicola, and identified a novel metallothionein-like protein encoding gene SpMTL. SpMTL showed functional similarity to other known MT proteins and also to its orthologues from non-hyperaccumulators. However, 3 additional cysteine residues were observed in SpMTL and appeared to be hyperaccumulator-specific...
February 2, 2017: Plant, Cell & Environment
https://www.readbyqxmd.com/read/28152513/propiece-il-1%C3%AE-facilitates-the-growth-of-acute-t-lymphocytic-leukemia-cells-through-the-activation-of-nf-%C3%AE%C2%BAb-and-sp1
#13
Yinsheng Zhang, Xiao Yu, Dandan Lin, Lei Lei, Bo Hu, Fengzhang Cao, Yu Mei, Depei Wu, Haiyan Liu
Interleukin 1α (IL-1α) is a pro-inflammatory cytokine that possesses multiple immune-regulatory functions. It is mainly expressed as the cell-associated form and not actively secreted in healthy tissues. The intracellular IL-1α has been shown to be a chromatin-associated cytokine and can affect transcription. There are spontaneous expressions of IL-1α in acute lymphocytic leukemia (ALL) blasts. However, the role of nuclear-localized IL-1α in ALL is not clear. Here we showed that overexpression of the nuclear form of IL-1α (propiece IL-1α) could promote proliferation and reduce apoptosis of T-ALL cells...
February 1, 2017: Oncotarget
https://www.readbyqxmd.com/read/28146278/detection-and-manipulation-of-the-stress-response-protein-metallothionein
#14
Sadikshya Bhandari, Clare Melchiorre, Kristen Dostie, Debby Laukens, Lindsey Devisscher, Ariel Louwrier, Amy Thees, Michael A Lynes
Metallothioneins (MTs) are small molecular weight stress response proteins that play a central role as reservoir of essential divalent heavy metal cations such as zinc and copper, and also can diminish the effects of toxic heavy metals such as mercury and cadmium. Historically, MT has been considered to be an intracellular protein with roles to play in the management of heavy metals, as a regulator of cellular redox potential, and as a buffer of free radicals. Our recent studies have highlighted immunomodulatory role of MT in inflammatory diseases and also in the progression of metastatic cell movement...
February 1, 2017: Current Protocols in Toxicology
https://www.readbyqxmd.com/read/28140569/silac-based-quantitative-proteomic-analysis-unveiled-the-arsenite-induced-perturbation-of-multiple-pathways-in-human-skin-fibroblast-cells
#15
Fan Zhang, Yongsheng Xiao, Yinsheng Wang
Humans are exposed to arsenic species through inhalation, ingestion and dermal contact, which may lead to skin, liver, and bladder cancers as well as cardiovascular and neurological diseases. The mechanisms underlying the cytotoxic and carcinogenic effects of arsenic species, however, remain incompletely understood. To exploit the mechanisms of toxicity of As(III), we employed stable isotope labeling by amino acids in cell culture (SILAC) together with LC-MS/MS analysis to assess quantitatively the As(III)-induced perturbation of the entire proteome of cultured human skin fibroblast cells...
January 31, 2017: Chemical Research in Toxicology
https://www.readbyqxmd.com/read/28137574/quantitation-of-human-metallothionein-isoforms-in-cells-tissues-and-cerebrospinal-fluid-by-mass-spectrometry
#16
J B Shabb, W W Muhonen, A A Mehus
Metallothioneins (MTs) are a family of small, highly conserved, cysteine-rich metal-binding proteins that are important for zinc and copper homeostasis, protection against oxidative stress, and buffering against toxic heavy metals. Individual human MT isoforms are candidate biomarkers for heavy metal toxicity, and selected cancers and neurodegenerative diseases. The similar antigenicity of human MT-1 and MT-2 isoforms precludes development of antibody-based assays for their individual quantitation. Metal-based MT quantitation methods do not directly measure MT isoforms...
2017: Methods in Enzymology
https://www.readbyqxmd.com/read/28134760/influence-of-transgenic-metallothionein-1-on-gliosis-ca1-neuronal-loss-and-brain-metal-levels-of-the-tg2576-mouse-model-of-alzheimer-s-disease
#17
Gemma Comes, Yasmina Manso, Anna Escrig, Olaya Fernandez-Gayol, Paula Sanchis, Amalia Molinero, Mercedes Giralt, Javier Carrasco, Juan Hidalgo
The mouse model of Alzheimer's disease (AD), Tg2576 mice (APP), has provided valuable information, such as the role of the metallothionein (MT) family in their behavioral and amyloidosis phenotypes. In this study, we further characterize the role of MT-1 by crossing Mt1-overexpressing mice with Tg2576 mice (APPTgMT). In 14-month-old mice, MT-1(/2) protein levels were dramatically increased by Mt1 overexpression throughout the cortex (Cx), which showed a prominent caudal-rostral gradient, and the hippocampus (HC)...
January 26, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28132138/tolerance-and-stress-response-of-sclerotiogenic-aspergillus-oryzae-g15-to-copper-and-lead
#18
Dan-Dan Long, Rong-Rong Fu, Jian-Rong Han
Aspergillus oryzae G15 was cultured on Czapek yeast extract agar medium containing different concentrations of copper and lead to investigate the mechanisms sustaining metal tolerance. The effects of heavy metals on biomass, metal accumulation, metallothionein (MT), malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD) were evaluated. Cu and Pb treatment remarkably delayed sclerotial maturation and inhibited mycelial growth, indicating the toxic effects of the metals. Cu decreased sclerotial biomass, whereas Pb led to an increase in sclerotial biomass...
January 29, 2017: Folia Microbiologica
https://www.readbyqxmd.com/read/28130743/effects-of-zinc-glycinate-on-productive-and-reproductive-performance-zinc-concentration-and-antioxidant-status-in-broiler-breeders
#19
Ling Zhang, Yong-Xia Wang, Xue Xiao, Jiang-Shui Wang, Qian Wang, Kai-Xuan Li, Tian-Yu Guo, Xiu-An Zhan
An experiment was conducted to investigate the effects of zinc glycinate (Zn-Gly) supplementation as an alternative for zinc sulphate (ZnSO4) on productive and reproductive performance, zinc (Zn) concentration and antioxidant status in broiler breeders. Six hundred 39-week-old Lingnan Yellow broiler breeders were randomly assigned to 6 groups consisting of 4 replicates with 25 birds each. Breeders were fed a basal diet (control group, 24 mg Zn/kg diet), basal diet supplemented with 80 mg Zn/kg diet from ZnSO4 or basal diet supplemented with 20, 40, 60 and 80 mg Zn/kg diet from Zn-Gly...
January 27, 2017: Biological Trace Element Research
https://www.readbyqxmd.com/read/28126494/metallothionein-protects-against-isoniazid-induced-liver-injury-through-the-inhibition-of-cyp2e1-dependent-oxidative-and-nitrosative-impairment-in-mice
#20
Yong Lian, Jing Zhao, Yi-Mei Wang, Jun Zhao, Shuang-Qing Peng
Oxidative stress mediated by hepatic CYP2E1 during isoniazid (INH) metabolism is considered responsible for INH hepatotoxicity. This study attempts to determine whether metallothionein (MT), a cysteine-rich antioxidant can protect against INH-induced liver injury by using a MT-I/II deficient mouse model (MT-/- mice). MT-/- mice and the corresponding wild-type mice received intragastric administrations of 0, 75, 150 and 300 mg/kg of INH for 15 days. The results showed that 150 and 300 mg/kg of INH induced liver injury in both types of mice, as evidenced by increased liver index and histopathological change of liver vacuolar degeneration...
January 23, 2017: Food and Chemical Toxicology
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