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metallothioneins

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https://www.readbyqxmd.com/read/28431387/cypermethrin-toxication-leads-to-histopathological-lesions-and-induces-inflammation-and-apoptosis-in-common-carp-cyprinus-carpio-l
#1
Harun Arslan, Selçuk Özdemir, Serdar Altun
Cypermethrin (Cyp), a known neurotoxic pesticide, is widely used in agricultural applications. In the present study, the aim was to determine the histopathological effects of Cyp toxication and evaluate the activation of inducible nitric oxide synthetase (iNOS) and 8-hydroxy-2-deoxyguanosine (8-OHdG) using an immunofluorescence assay. Thereafter, we identified the expressions of caspase 3, capsase 8, iNOS, and metallothionein 1 (MT1) genes in common carp using quantitative reverse transcription polymerase chain reaction (qRT-PCR)...
April 14, 2017: Chemosphere
https://www.readbyqxmd.com/read/28428350/targeted-inactivation-of-copper-transporter-atp7b-in-hepatocytes-causes-liver-steatosis-and-obesity-in-mice
#2
Abigael Muchenditsi, Haojun Yang, James P Hamilton, Lahari Koganti, Franck Housseau, Lisa Aronov, Hongni Fan, Hannah Pierson, Ashima Bhattacharjee, Robert C Murphy, Cynthia L Sears, James J Potter, Clavia Ruth Wooton-Kee, Svetlana Lutsenko
The copper transporter ATP7B is essential for mammalian copper homeostasis. Mutations in ATP7B result in copper accumulation, especially in the liver, and cause Wilson disease (WD). The major role of hepatocytes in WD pathology is firmly established. It is less certain whether the excess Cu in hepatocytes is solely responsible for development of WD. To address this issue, we generated a mouse strain for Cre-mediated deletion of Atp7b and inactivated Atp7b selectively in hepatocytes. Atp7bΔ(Hep) mice accumulate copper in the liver, have elevated urinary copper, lack holo-ceruloplasmin, but show no liver disease for up to 30 weeks...
April 20, 2017: American Journal of Physiology. Gastrointestinal and Liver Physiology
https://www.readbyqxmd.com/read/28426746/low-levels-of-cd-induce-persisting-epigenetic-modifications-and-acclimation-mechanisms-in-the-earthworm-lumbricus-terrestris
#3
Maja Šrut, Victoria Drechsel, Martina Höckner
Toxic effects of cadmium (Cd), a common soil pollutant, are still not very well understood, particularly in regard to its epigenetic impact. Therefore, the aim of this study was to assess DNA methylation changes and their persistence in the earthworm Lumbricus terrestris upon chronic low dose Cd exposure using methylation sensitive amplification polymorphism (MSAP). Moreover, the biomarker response and fitness of the earthworms, as well as the expression of detoxification-related genes (metallothionein (MT) and phytochelatin synthase (PCS)) was evaluated...
2017: PloS One
https://www.readbyqxmd.com/read/28426713/metallothioneins-regulate-the-adipogenic-differentiation-of-3t3-l1-cells-via-the-insulin-signaling-pathway
#4
Yoshito Kadota, Yuriko Toriuchi, Yuka Aki, Yuto Mizuno, Takashige Kawakami, Tomoko Nakaya, Masao Sato, Shinya Suzuki
Knockout of metallothionein (MT) genes contributes to a heavier body weight in early life and the potential to become obese through the intake of a high fat diet (HFD) in mice. It has thus been suggested that MT genes regulate the formation of adipose tissue, which would become the base for later HFD-induced obesity. We evaluated the fat pads of mice during the lactation stage. The fat mass and adipocyte size of MT1 and MT2 knockout mice were greater than those of wild type mice. Next, we assayed the ability of small interfering RNA (siRNA) to silence MT genes in the 3T3-L1 cell line...
2017: PloS One
https://www.readbyqxmd.com/read/28422390/naringin-abates-adverse-effects-of-cadmium-mediated-hepatotoxicity-an-experimental-study-using-hepg2-cells
#5
Visesh Kumar Rathi, Shubhankar Das, Archana Parampalli Raghavendra, Bola Sadashiva Satish Rao
This study investigated the protective potential of Naringin (NIN) against cadmium chloride (CdCl2 ) mediated hepatotoxicity using human hepatocellular carcinoma (HepG2) cells. An optimal concentration of NIN (5 μM) was potent enough to confer cytoprotection against CdCl2 (50 μM) as was observed by MTT assay. Preconditioning with NIN maintained redox homeostasis, mitochondrial membrane potential, and reduced apoptosis as marked by decrease in the percentage sub-G0 /G1 and Annexin V-FITC/propidium iodide positive cells (apoptotic)...
April 19, 2017: Journal of Biochemical and Molecular Toxicology
https://www.readbyqxmd.com/read/28420950/metallothionein-copper-and-alpha-synuclein-in-alpha-synucleinopathies
#6
REVIEW
Yuho Okita, Alexandre N Rcom-H'cheo-Gauthier, Michael Goulding, Roger S Chung, Peter Faller, Dean L Pountney
Metallothioneins (MTs) are proteins that function by metal exchange to regulate the bioavailability of metals, such as zinc and copper. Copper functions in the brain to regulate mitochondria, neurotransmitter production, and cell signaling. Inappropriate copper binding can result in loss of protein function and Cu(I)/(II) redox cycling can generate reactive oxygen species. Copper accumulates in the brain with aging and has been shown to bind alpha-synuclein and initiate its aggregation, the primary aetiological factor in Parkinson's disease (PD), and other alpha-synucleinopathies...
2017: Frontiers in Neuroscience
https://www.readbyqxmd.com/read/28419969/neurotoxic-effects-of-silver-nanoparticles-and-the-protective-role-of-rutin
#7
Mona M Ahmed, Mohamed M A Hussein
The toxicological studies on silver nanoparticles (Ag-NPs) have become a hot topic over the past few decades due to their unique properties on the nanoscale and widespread in many commercial products that launched into the market recently. This study was undertaken to shed light on Ag-NPs toxicity on neurotransmitters with special emphasis on the impact of concurrent administration of rutin with Ag-NPs in the experimental rats. The oral administration of Ag-NPs in rats induced brain oxidative stress, significant alterations in neurotransmitters and amino acids...
April 15, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28417916/metallothionein-gene-family-in-the-sea-urchin-paracentrotus-lividus-gene-structure-differential-expression-and-phylogenetic-analysis
#8
Maria Antonietta Ragusa, Aldo Nicosia, Salvatore Costa, Angela Cuttitta, Fabrizio Gianguzza
Metallothioneins (MT) are small and cysteine-rich proteins that bind metal ions such as zinc, copper, cadmium, and nickel. In order to shed some light on MT gene structure and evolution, we cloned seven Paracentrotus lividus MT genes, comparing them to Echinodermata and Chordata genes. Moreover, we performed a phylogenetic analysis of 32 MTs from different classes of echinoderms and 13 MTs from the most ancient chordates, highlighting the relationships between them. Since MTs have multiple roles in the cells, we performed RT-qPCR and in situ hybridization experiments to understand better MT functions in sea urchin embryos...
April 12, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28417197/origin-function-and-fate-of-metallothionein-in-human-blood
#9
Mohammad Tariqur Rahman, Nazmul Haque, Noor Hayaty Abu Kasim, Marc De Ley
Toxic heavy metals, toxic organic compounds, reactive oxygen species (ROS), infections, and temperature are well-known metallothionein (MT) inducers in human blood. The current review aims to summarize synthesis, function, and fate of human blood MT in response to the known MT inducers. Part of the MTs that are synthesized in different organs such as the liver, kidney, and spleen is transported and stored in different blood cells and in plasma. Cells of the circulatory system also synthesize MT. From the circulation, MT returns to the kidney where the metal-bound MTs are degraded to release the metal ion that in turn induces MT expression therein...
April 18, 2017: Reviews of Physiology, Biochemistry and Pharmacology
https://www.readbyqxmd.com/read/28407590/exposure-to-lethal-levels-of-benzo-a-pyrene-or-cadmium-trigger-distinct-protein-expression-patterns-in-earthworms-eisenia-fetida
#10
Lihao Zhang, Xiaochen Duan, Nannan He, Xu Chen, Jinli Shi, Weiming Li, Li Xu, Huixin Li
Different pollutants induce distinct toxic responses in earthworms (Eisenia fetida). Here, we used proteomics techniques to compare the responses of E. fetida to exposure to the 10% lethal concentration (14d-LC10) of benzo[a]pyrene (BaP) or cadmium (Cd) in natural red soil (China). BaP exposure markedly induced the expression of oxidation-reduction proteins, whereas Cd exposure mainly induced the expression of proteins involved in transcription- and translation-related processes. Furthermore, calmodulin-binding proteins were differentially expressed upon exposure to different pollutants...
April 10, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28406445/morphofunctional-alterations-in-zebrafish-danio-rerio-gills-after-exposure-to-mercury-chloride
#11
Rachele Macirella, Elvira Brunelli
Mercury (Hg) is a global pollutant that may exert its toxic effects on living organisms and is found in both aquatic and terrestrial ecosystems in three chemical forms; elemental, organic, and inorganic. The inorganic form (iHg) tends to predominantly accumulate in aquatic environments. The gill apparatus is a very dynamic organ that plays a fundamental role in gas exchange, osmoregulation, acid-base regulation, detoxification, and excretion, and the gills are the primary route of waterborne iHg entrance in fish...
April 13, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28399865/toxicity-of-nano-and-ionic-silver-to-embryonic-stem-cells-a-comparative-toxicogenomic-study
#12
Xiugong Gao, Vanessa D Topping, Zachary Keltner, Robert L Sprando, Jeffrey J Yourick
BACKGROUND: The widespread application of silver nanoparticles (AgNPs) and silver-containing products has raised public safety concerns about their adverse effects on human health and the environment. To date, in vitro toxic effects of AgNPs and ionic silver (Ag(+)) on many somatic cell types are well established. However, no studies have been conducted hitherto to evaluate their effect on cellular transcriptome in embryonic stem cells (ESCs). RESULTS: The present study characterized transcriptomic changes induced by 5...
April 11, 2017: Journal of Nanobiotechnology
https://www.readbyqxmd.com/read/28399854/improved-n-butanol-production-via-co-expression-of-membrane-targeted-tilapia-metallothionein-and-the-clostridial-metabolic-pathway-in-escherichia-coli
#13
Wei-Chih Chin, Kuo-Hsing Lin, Chun-Chi Liu, Kenji Tsuge, Chieh-Chen Huang
BACKGROUND: N-Butanol has favorable characteristics for use as either an alternative fuel or platform chemical. Bio-based n-butanol production using microbes is an emerging technology that requires further development. Although bio-industrial microbes such as Escherichia coli have been engineered to produce n-butanol, reactive oxygen species (ROS)-mediated toxicity may limit productivity. Previously, we show that outer-membrane-targeted tilapia metallothionein (OmpC-TMT) is more effective as an ROS scavenger than human and mouse metallothioneins to reduce oxidative stress in the host cell...
April 11, 2017: BMC Biotechnology
https://www.readbyqxmd.com/read/28395775/lc-coupled-to-esi-maldi-and-icp-ms-a-multiple-hyphenation-for-metalloproteomic-studies
#14
Kateřina Coufalíková, Iva Benešová, Tomáš Vaculovič, Viktor Kanický, Jan Preisler
A new multiple detection arrangement for liquid chromatography (LC) that supplements conventional electrospray ionization (ESI) mass spectrometry (MS) detection with two complementary detection techniques, matrix-assisted laser desorption/ionization (MALDI) MS and substrate-assisted laser desorption inductively coupled plasma (SALD ICP) MS has been developed. The combination of the molecular and elemental detectors in a single separation run is accomplished by utilizing a commercial MALDI target made of conductive plastic...
May 22, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28395246/electrochemical-and-optical-study-of-metallothionein-interactions-with-prion-proteins
#15
Alzbeta Cardova, Pavlina Adam, Stefano Mariani, Lukas Richtera, Zbynek Heger, Jan Labuda, Maria Minunni, Vojtech Adam
The prion protein (PrP(C)) can be structurally shifted to its PrP(Sc) isoform causing a wide range of neurodegenerative diseases, which are currently incurable. There is an evidence that metallothioneins (MTs), and especially MT-3, are associated with neurodegenerative diseases. PrP(C) and MTs play pivotal roles in maintaining metal homeostasis; therefore, it is conceivable that each of them has its own significance in prion diseases. In this paper, we study the nature of interactions between PrP(C), MT, and copper ions, Cu(II), using the method of differential pulse voltammetry (DPV) coupled with adsorptive transfer stripping technique (AdTS)...
March 23, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28393194/metallothionein-1g-promotes-the-differentiation-of-ht-29-human-colorectal-cancer-cells
#16
Juan Martín Arriaga, Alicia Inés Bravo, José Mordoh, Michele Bianchini
Metallothioneins (MTs) are a family of low-molecular-weight, cysteine-rich proteins involved in zinc and redox metabolism, that are epigenetically downregulated during colorectal cancer (CRC) progression, but may be re-induced with a variety of agents. Since loss of MT expression is associated with a worse prognosis, in the present study we investigated the effects of overexpression of the most significantly downregulated isoform in CRC, namely MT1G, on the HT-29 cell line. Overexpression of MT1G resulted in xenograft tumors with an aberrant morphology, characterized by an evident increase in mucin-containing cells that were identified as goblet cells under electron microscopy...
May 2017: Oncology Reports
https://www.readbyqxmd.com/read/28391890/the-association-of-the-blood-lead-level-and-serum-lipid-concentrations-may-be-modified-by-the-genetic-combination-of-the-metallothionein-2a-polymorphisms-rs10636%C3%A2-gc-and-rs28366003-aa
#17
Chen-Cheng Yang, Chih-Shien Chuang, Chia-I Lin, Chao-Ling Wang, Yung-Cheng Huang, Hung-Yi Chuang
BACKGROUND: Lead in blood can stimulate lipid oxidation in phosphatidylcholine and increase peroxidation in lipids. Metallothionein (MT) is a cysteine-rich protein that can influence the detoxification of heavy metals and scavenge oxidative stress for free radicals. One of the most expressive functional genes in humans is the MT2A gene. OBJECTIVE: This study aims to determine if the association of the blood lead level and lipid biomarkers was influenced by MT2A polymorphisms...
January 2017: Journal of Clinical Lipidology
https://www.readbyqxmd.com/read/28381587/properties-of-mitotic-and-meiotic-recombination-in-the-tandemly-repeated-cup1-gene-cluster-in-the-yeast-saccharomyces-cerevisiae
#18
Ying Zhao, Margaret Dominska, Aleksandra Petrova, Halle Bagshaw, Robert J Kokoska, Thomas D Petes
In the yeast Saccharomyces cerevisiae, the genes encoding the metallothionein protein Cup1 are located in a tandem array on chromosome VIII. Using a diploid strain that is heterozygous for an insertion of a selectable marker (URA3) within this tandem array, and heterozygous for markers flanking the array, we measured inter-homolog recombination and intra-/sister-chromatid exchange in the CUP1 locus. The rate of intra-/sister chromatid recombination exceeded the rate of inter-homolog recombination by more than ten-fold...
April 4, 2017: Genetics
https://www.readbyqxmd.com/read/28380433/metallothionein-1m-suppresses-tumorigenesis-in-hepatocellular-carcinoma
#19
Cheng-Lin Fu, Bing Pan, Ju-Hua Pan, Mei-Fu Gan
Members of the metallothionein (MT) family are involved in metal detoxifcation and in the protection of cells against certain electrophilic carcinogens. In present study, it was found that MT1M was downregulated in more than 77.1% (91/118) of hepatocellular carcinoma (HCC) tissues compared with adjacent non-tumor tissues. Furthermore, overexpression of MT1M inhibited cell viability, colony formation, cell migration and invasion in HCC cell lines and tumor cell growth in xenograft nude mice, and activated cell apoptosis in HCC cell lines...
March 23, 2017: Oncotarget
https://www.readbyqxmd.com/read/28370392/extremely-low-frequency-electromagnetic-field-induces-neural-differentiation-of-hbm-mscs-through-regulation-of-zn-metallothionein-3
#20
Anastasia Rosebud Aikins, Sung-Won Hong, Hyun-Jung Kim, Cheol-Ho Yoon, Joo-Hee Chung, MiJung Kim, Chan-Wha Kim
Extremely low-frequency electromagnetic field (ELFEMF) can stimulate neural differentiation in human bone marrow-derived mesenchymal cells (hBM-MSCs), and this provides an opportunity for research on neurodegenerative diseases such as Alzheimer's disease (AD). Metallothionein-3 (MT3), an isoform of the metal-binding proteins, metallothioneins, involved in maintaining intracellular zinc (Zn) homeostasis and the deregulation of zinc homeostasis, has separately been implicated in AD. Here, we investigated the effect of ELFEMF-induced neural differentiation of hBM-MSCs on Zn-MT3 homeostatic interaction...
March 29, 2017: Bioelectromagnetics
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