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https://www.readbyqxmd.com/read/28738528/molecular-basis-for-oxidative-stress-induced-by-simulated-microgravity-in-nematode-caenorhabditis-elegans
#1
Li Zhao, Qi Rui, Dayong Wang
Caenorhabditis elegans is an important in vivo assay system for toxicological studies. Herein, we investigated the role of oxidative stress and the underlying molecular mechanism for induced adverse effects of simulated microgravity. In nematodes, simulated microgravity treatment induced a significant induction of oxidative stress. Genes (mev-1, gas-1, and isp-1) encoding a molecular machinery for the control of oxidative stress were found to be dysregulated in simulated microgravity treated nematodes. Meanwhile, genes (sod-2, sod-3, sod-4, sod-5, aak-2, skn-1, and gst-4) encoding certain antioxidant defense systems were increased in simulated microgravity treated nematodes...
July 19, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28735517/metallothionein-expression-and-synthesis-in-the-testis-of-the-lizard-podarcis-sicula-under-natural-conditions-and-following-estrogenic-exposure
#2
Mariailaria Verderame, Ermelinda Limatola, Rosaria Scudiero
Metallothionein (MT) is the main protein involved in the homeostasis of metallic micronutrients and in cellular defence against heavy metals and reactive oxygen species. Found in almost all vertebrate tissues, MT presence and localization in the testis has been controversial. In the present study, by using in situ hybridization and immunohistochemical analysis we assessed the localization of both MT transcript and protein in Podarcis sicula testes during two different phases of the reproductive cycle: the autumnal resumption and the spring-early summer mating period...
May 3, 2017: European Journal of Histochemistry: EJH
https://www.readbyqxmd.com/read/28734967/assessment-of-complex-water-pollution-with-heavy-metals-and-pyrethroid-pesticides-on-transcript-levels-of-metallothionine-and-immune-related-genes
#3
Haneen A Ghazy, Mohamed A S Abdel-Razek, Abeer F El Nahas, Shawky Mahmoud
Alteration of immunological function of an aquatic organism can be used as an indicator for evaluating the direct effect of exposure to pollutants. The aim of this work is to assess the impact of complex water pollution with special reference to Pyrethroid pesticides and heavy metals on mRNA transcript levels of Metallothionine and some immune related genes of Nile tilapia (Oreochromas Niloticus). Residues of six heavy metals and six Pyrethroid were assessed in water as well as fish tissues at three different sites of Lake Burullus, located at Northern Egypt...
July 19, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28731302/lead-ii-binding-in-metallothioneins
#4
Daisy L Wong, Maureen E Merrifield-MacRae, Martin J Stillman
Heavy metal exposure has long been associated with metallothionein (MT) regulation and its functions. MT is a ubiquitous, cysteine-rich protein that is involved in homeostatic metal response for the essential metals zinc and copper, as well as detoxification of heavy metals; the most commonly proposed being cadmium. MT binds in vivo to a number of metals in addition to zinc, cadmium and copper, such as bismuth. In vitro, metallation with a wide range of metals (especially mercury, arsenic, and lead) has been reported using a variety of analytical methods...
April 10, 2017: Metal Ions in Life Sciences
https://www.readbyqxmd.com/read/28728575/genome-wide-association-study-identifies-a-major-gene-for-beech-bark-disease-resistance-in-american-beech-fagus-grandifolia-ehrh
#5
Irina Ćalić, Jennifer Koch, David Carey, Charles Addo-Quaye, John E Carlson, David B Neale
BACKGROUND: The American Beech tree (Fagus grandifolia Ehrh.), native to eastern North America, is ecologically important and provides high quality wood products. This species is susceptible to beech bark disease (BBD) and is facing high rates of mortality in North America. The disease occurs from an interaction between the woolly beech scale insect (Cryptococcus fagisuga), one of two species of the fungus Neonectria (N. faginata or N. ditissima), and American Beech trees. METHODS: In this case-control genome-wide association study (GWAS), we tested 16 K high quality SNPs using the Affymetrix Axiom 1...
July 20, 2017: BMC Genomics
https://www.readbyqxmd.com/read/28722145/dendrobium-nobile-lindl-alkaloids-regulate-metabolism-gene-expression-in-livers-of-mice
#6
Yun-Yan Xu, Ya-Sha Xu, Yuan Wang, Qin Wu, Yuan-Fu Lu, Jie Liu, Jing-Shan Shi
OBJECTIVES: In our previous studies, Dendrobium nobile Lindl. alkaloids (DNLA) has been shown to have glucose-lowering and antihyperlipidaemia effects in diabetic rats, in rats fed with high-fat diets, and in mice challenged with adrenaline. This study aimed to examine the effects of DNLA on the expression of glucose and lipid metabolism genes in livers of mice. METHODS: Mice were given DNLA at doses of 10-80 mg/kg, po for 8 days, and livers were removed for total RNA and protein isolation to perform real-time RT-PCR and Western blot analysis...
July 19, 2017: Journal of Pharmacy and Pharmacology
https://www.readbyqxmd.com/read/28718783/metallothionein-from-wild-populations-of-the-african-catfish-clarias-gariepinus-from-sequence-protein-expression-and-metal-binding-properties-to-transcriptional-biomarker-of-metal-pollution
#7
Ethel M'kandawire, Agnieszka Mierek-Adamska, Stephen R Stürzenbaum, Kennedy Choongo, John Yabe, Maxwell Mwase, Ngonda Saasa, Claudia A Blindauer
Anthropogenic pollution with heavy metals is an on-going concern throughout the world, and methods to monitor release and impact of heavy metals are of high importance. With a view to probe its suitability as molecular biomarker of metal pollution, this study has determined a coding sequence for metallothionein of the African sharptooth catfish Clarias gariepinus. The gene product was recombinantly expressed in Escherichia coli in presence of Zn(II), Cd(II), or Cu, and characterised by Electrospray Ionisation Mass Spectrometry and elemental analysis...
July 18, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28712825/use-of-biomarkers-to-evaluate-the-effects-of-environmental-stressors-on-mytilus-galloprovincialis-sampled-along-the-moroccan-coasts-integrating-biological-and-chemical-data
#8
Bouchra El Haimeur, Mina Bouhallaoui, Mariama Zbiry, Najat Elkhiati, Sophia Talba, Susanna Sforzini, Aldo Viarengo, Ali Benhra
A biomonitoring study using wild Mytilus galloprovincialis mussels sampled from six sites along the Moroccan coasts evaluated whether select biomarkers are suitable for identifying and quantifying pollution-induced stress syndrome in mussels. Lysosomal membrane stability was confirmed to be a highly sensitive biological parameter, and acetylcholinesterase activity was found a suitable biomarker of neurotoxicity. Metallothionein concentrations were in line with heavy metal concentrations detected in mussel tissues...
June 13, 2017: Marine Environmental Research
https://www.readbyqxmd.com/read/28711631/corrigendum-to-osteopontin-metallothionein-i-ii-interactions-in-experimental-autoimmune-encephalomyelitis-neuroscience-350-2017-133-145
#9
Hrvoje Jakovac, Tanja Grubić Kezele, Sandrašuć Urović, Biserka Mulac-Jeričević, Biserka Radošević-Stašić
No abstract text is available yet for this article.
July 13, 2017: Neuroscience
https://www.readbyqxmd.com/read/28704692/heavy-metal-pollution-due-to-coal-washery-effluent-and-its-decontamination-using-a-macrofungus-pleurotus-ostreatus
#10
Huma Vaseem, V K Singh, M P Singh
Release of the effluent generated during washing of coal became a major problem for coal industries due to presence of many toxic metals and other pollutants. These effluents are the main source of pollution in rivers and other water bodies. Therefore in this study, we tried to analyse the toxicity of coal washery effluent (CWE) collected from one of the washery plant situated in Dhanbad, India. CWE was found to be very toxic containing large amount of suspended particles and many heavy metals above than their permissible limits...
July 10, 2017: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/28704659/preheating-mitigates-cadmium-toxicity-in-zebrafish-livers-evidence-from-promoter-demethylation-gene-transcription-to-biochemical-levels
#11
Jia-Lang Zheng, Sai-Nan Guo, Shuang-Shuang Yuan, Hu Xia, Qing-Ling Zhu, Zhen-Ming Lv
The working hypothesis for this study was that moderate heat stress would alleviate the deleterious effects of subsequent cadmium (Cd) exposure on fish. Thus, zebrafish (Danio rerio) were subjected to water maintained at 26°C and 34°C for 4days, and then exposed to 0 or 200μg/L Cd for 1 week at 26°C. Multiple indicators were measured from livers of zebrafish at different levels, including DNA, RNA, protein and enzymatic activity associated with oxidative stress, inflammation and metal transport. The ameliorative effect of preheatinging on Cd toxicity was demonstrated...
June 26, 2017: Aquatic Toxicology
https://www.readbyqxmd.com/read/28704512/a-role-for-the-drosophila-zinc-transporter-zip88e-in-protecting-against-dietary-zinc-toxicity
#12
Christopher D Richards, Coral G Warr, Richard Burke
Zinc absorption in animals is thought to be regulated in a local, cell autonomous manner with intestinal cells responding to dietary zinc content. The Drosophila zinc transporter Zip88E shows strong sequence similarity to Zips 42C.1, 42C.2 and 89B as well as mammalian Zips 1, 2 and 3, suggesting that it may act in concert with the apically-localised Drosophila zinc uptake transporters to facilitate dietary zinc absorption by importing ions into the midgut enterocytes. However, the functional characterisation of Zip88E presented here indicates that Zip88E may instead play a role in detecting and responding to zinc toxicity...
2017: PloS One
https://www.readbyqxmd.com/read/28692912/effects-of-molybdenum-and-cadmium-on-the-oxidative-damage-and-kidney-apoptosis-in-duck
#13
Lele Shi, Huabin Cao, Junrong Luo, Ping Liu, Tiancheng Wang, Guoliang Hu, Caiying Zhang
Molybdenum (Mo) is an essential element for human beings and animals; however, high dietary intake of Mo can lead to adverse reactions. Cadmium (Cd) is one of the major transitional metals which has toxic effects in animals. To investigate the co-induced toxic effects of Mo and Cd on oxidative damage and kidney apoptosis in duck, 120 ducks were randomly divided into control group and 5 treatment groups which were treated with a commercial diet containing different dosages of Mo and Cd. Kidney samples were collected on the 60th and 120th days to determine the mRNA expression levels of ceruloplasmin (CP), metallothionein (MT), Bak-1, and Caspase-3 by quantitative RT-PCR...
July 7, 2017: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/28689713/antioxidant-defense-system-and-oxidative-status-in-antarctic-fishes-the-sluggish-rockcod-notothenia-coriiceps-versus-the-active-marbled-notothen-notothenia-rossii
#14
Roberta Daniele Klein, Carlos Eduardo Rosa, Elton Pinto Colares, Ricardo Berteaux Robaldo, Pablo Elias Martinez, Adalto Bianchini
Adaptive responses of antioxidant defense systems (ADS) to changes in increased levels of activity are critical, especially in Antarctic fishes. The benthopelagic marbled notothen (Notothenia rossii) shows higher spontaneous activity than the benthonic and sluggish rockcod (N. coriiceps). Therefore, we hypothesize that species-related responses of ADS would occur to counteract different rates of reactive oxygen species formation in these two Antarctic fish. Here we evaluated ADS and oxidative damage in tissues (brain, gills, liver and white muscle) of the two Antarctic fish...
August 2017: Journal of Thermal Biology
https://www.readbyqxmd.com/read/28689712/effects-of-increasing-temperature-on-antioxidant-defense-system-and-oxidative-stress-parameters-in-the-antarctic-fish-notothenia-coriiceps-and-notothenia-rossii
#15
Roberta Daniele Klein, Vinicius Dias Borges, Carlos Eduardo Rosa, Elton Pinto Colares, Ricardo Berteaux Robaldo, Pablo Elias Martinez, Adalto Bianchini
Antioxidant defense system (ADS) and oxidative stress parameters were evaluated in the Antarctic fish Notothenia rossii and N. coriiceps exposed to increasing temperature. Acclimated fish were kept at 0°C or exposed to 4°C for 1day (N. rossii) or to 2 and 4°C for 1 and 6 days (N. coriiceps). Measurements were assessed in brain, gills, liver, white muscle and erythrocytes. Parameters analyzed included antioxidant capacity against peroxyl radicals (ACAP); reduced glutathione (GSH) and metallothionein-like proteins (MTLP) concentration; superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione-S-transferase (GST) and glutamate-cysteine ligase (GCL) activity; lipid peroxidation (LPO) level and protein carbonyl (PC) concentration...
August 2017: Journal of Thermal Biology
https://www.readbyqxmd.com/read/28687269/chemopreventive-effects-of-a-tamarindus-indica-fruit-extract-against-colon-carcinogenesis-depends-on-the-dietary-cholesterol-levels-in-hamsters
#16
Flavia Martinello, Vinicius Kannen, João José Franco, Bianca Gasparotto, Juliana Yumi Sakita, Atushi Sugohara, Sergio Britto Garcia, Sergio Akira Uyemura
Tamarind has significant antioxidant potential. We showed that tamarind protects hypercholesterolemic hamsters from atherosclerosis. Hypercholesterolemia might increase the risk of colon cancer. We investigated whether tamarind extract modulates the risk of colon cancer in hypercholesterolemic hamsters. Hamsters (n = 64) were given tamarind and a hypercholesterolemic diet for 8 weeks. The groups were the control, tamarind treatment, hypercholesterolemic, and hypercholesterolemic treated with tamarind groups...
July 4, 2017: Food and Chemical Toxicology
https://www.readbyqxmd.com/read/28684706/biomphalaria-glabrata-metallothionein-lacking-metal-specificity-of-the-protein-and-missing-gene-upregulation-suggest-metal-sequestration-by-exchange-instead-of-through-selective-binding
#17
Michael Niederwanger, Sara Calatayud, Oliver Zerbe, Sílvia Atrian, Ricard Albalat, Mercè Capdevila, Òscar Palacios, Reinhard Dallinger
The wild-type metallothionein (MT) of the freshwater snail Biomphalaria glabrata and a natural allelic mutant of it in which a lysine residue was replaced by an asparagine residue, were recombinantly expressed and analyzed for their metal-binding features with respect to Cd(2+), Zn(2+) and Cu⁺, applying spectroscopic and mass-spectrometric methods. In addition, the upregulation of the Biomphalaria glabrataMT gene was assessed by quantitative real-time detection PCR. The two recombinant proteins revealed to be very similar in most of their metal binding features...
July 6, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28684668/analysis-of-metal-binding-features-of-the-wild-type-and-two-domain-truncated-mutant-variants-of-littorina-littorea-metallothionein-reveals-its-cd-specific-character
#18
Òscar Palacios, Elena Jiménez-Martí, Michael Niederwanger, Selene Gil-Moreno, Oliver Zerbe, Sílvia Atrian, Reinhard Dallinger, Mercè Capdevila
After the resolution of the 3D structure of the Cd₉-aggregate of the Littorina littorea metallothionein (MT), we report here a detailed analysis of the metal binding capabilities of the wild type MT, LlwtMT, and of two truncated mutants lacking either the N-terminal domain, Lltr2MT, or both the N-terminal domain, plus four extra flanking residues (SSVF), Lltr1MT. The recombinant synthesis and in vitro studies of these three proteins revealed that LlwtMT forms unique M₉-LlwtMT complexes with Zn(II) and Cd(II), while yielding a complex mixture of heteronuclear Zn,Cu-LlwtMT species with Cu(I)...
July 6, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28671998/metal-contamination-in-harbours-impacts-life-history-traits-and-metallothionein-levels-in-snails
#19
Maria Alexandra Bighiu, Elena Gorokhova, Bethanie Carney Almroth, Ann-Kristin Eriksson Wiklund
Harbours with limited water exchange are hotspots of contaminant accumulation. Antifouling paints (AF) contribute to this accumulation by leaching biocides that may affect non-target species. In several leisure boat harbours and reference areas in the Baltic Sea, chronic exposure effects were evaluated using caging experiments with the snail Theodoxus fluviatilis. We analysed variations in ecologically relevant endpoints (mortality, growth and reproduction) in concert with variation in metallothionein-like proteins (MTLP) levels...
2017: PloS One
https://www.readbyqxmd.com/read/28662978/concentrations-of-metallic-elements-in-kidney-liver-and-lung-tissue-of-indo-pacific-bottlenose-dolphin-tursiops-aduncus-from-coastal-waters-of-zanzibar-tanzania
#20
Edgar C Mapunda, Othman C Othman, Leonard D Akwilapo, Hindrik Bouwman, Haji Mwevura
Concentrations of metallic elements in kidney, liver and lung tissues of Indo-Pacific bottlenose dolphins Tursiops aduncus from coastal waters of Zanzibar were determined using inductively coupled plasma - optical emission spectroscopy. Cadmium, chromium, copper, and zinc were quantifiable in all tissues at concentration ranges of 0.10-150, 0.08-3.2, 1.1-88 and 14-210μg/g dry mass, respectively. Copper and zinc was significantly higher in liver, and females had significantly higher Cd in liver, and chromium in lung...
June 26, 2017: Marine Pollution Bulletin
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